CHE142 Worksheet 1 2T 2017-2018

Initial rates of an enzyme-catalyzed reaction for various substrate concentration are listed in
Table 1.
a.) Evaluate Km and vmax by a Lineweaver-Burk Plot.
b.) Using an Eadie Hofstee plot and evaluate vmax and Km
Sx
v (x 10^6) 10^4 v/s 1/S 1/v 1/S *10^3 1/v x10^3
mol/(L-m) mol/L
177.000 41.0000 0.04317 243.902 5649.718 0.24 5.65
173.000 9.5000 0.18211 1052.632 5780.347 1.05 5.78
125.000 5.2000 0.24038 1923.077 8000.000 1.92 8.00
106.000 1.0300 1.02913 9708.738 9433.962 9.71 9.43
80.000 0.4900 1.63265 20408.163 12500.000 20.41 12.50
67.000 0.1060 6.32075 94339.623 14925.373 94.34 14.93
43.000 0.0510 8.43137 196078.431 23255.814 196.08 23.26