Clotrimazole cream by hplc

Extract a quantity of the cream containing 20 mg of Clotrimazole by warming with 20 ml of methanol in a

water bath at 50 for 5 minutes, shaking occasionally. Remove from the water-bath, shake the mixture
vigorously while cooling to room temperature, cool in ice for 15 minutes, centrifuge for 5 minutes and
decant the supernatant liquid. Repeat the extraction with two further 20-ml quantities of methanol . To
the combined methanol extracts add 10 ml of methanol and dilute to 100 ml with 0.02M orthophosphoric
acid .bring to RT and dilute 1 volume of the filtrate to 5 volumes with a mixture of 70 volumes of
methanol and 30 volumes of 0.02M orthophosphoric acid

std

dissolve 20 mg of clotrimazole BPCRS in 70 ml of methanol , add sufficient 0.02M orthophosphoric acid

to produce 100 ml and dilute 1 volume of the resulting solution to 5 volumes with a mixture of 70 volumes
of methanol and 30 volumes of 0.02M orthophosphoric acid.

The chromatographic procedure may be carried out using (a) a stainless steel column (20 cm 4.6 mm)
packed with octadecylsilyl silica gel for chromatography (5 m) (Lichrosorb RP-18 or Spherisorb ODS 1
is suitable), (b) as the mobile phase with a flow rate of 1.5 ml per minute a mixture of 30 volumes of
0.02M orthophosphoric acid and 70 volumes of methanol , the pH of the mixture being adjusted to 7.5
with a 10% v/v solution of triethylamine in methanol and (c) a detection wavelength of 215 nm.