ProtParam

User-provided sequence:

10 20 30 40 50 60
MARFEEQKLY IGGRYVEASS GATFETINPA NGEVLAKVQR ASREDVERAV QSAVEGQKVW

70 80 90 100 110 120

AAMTAMQRSR ILRRAVDILR ERNDELAALE TLDTGKPLAE TRSVDIVTGA DVLEYYAGLV

130 140 150 160 170 180

PAIEGEQIPL RETSFVYTRR EPLGVVAGIG AWNYPVQIAL WKSAPALAAG NAMIFKPSEV

190 200 210 220 230 240

TPLTALKLAE IYTEAGVPDG VFNVLTGSGR EVGQWLTEHP LIEKISFTGG TSTGKKVMAS

250 260 270 280 290 300

ASSSSLKEVT MELGGKSPLI IFPDADLDRA ADIAVMANFF SSGQVCTNGT RVFIHRSQQA

310 320 330 340 350 360

RFEAKVLERV QRIRLGDPQD ENTNFGPLVS FPHMESVLGY IESGKAQKAR LLCGGERVTD

370 380 390 400 410 420

GAFGKGAYVA PTVFTDCRDD MTIVREEIFG PVMSILVYDD EDEAIRRAND TEYGLAAGVV

430 440 450 460 470 480

TQDLARAHRA IHRLEAGICW INTWGESPAE MPVGGYKQSG VGRENGLTTL AHYTRIKSVQ

490
VELGDYASVF

References and documentation are available.

Number of amino acids: 490

Molecular weight: 53332.4

Theoretical pI: 5.25

CSV format
Amino acid composition:
Ala (A) 54 11.0%
Arg (R) 34 6.9%
Asn (N) 13 2.7%
Asp (D) 23 4.7%
Cys (C) 4 0.8%
Gln (Q) 17 3.5%
Glu (E) 41 8.4%
Gly (G) 45 9.2%
His (H) 6 1.2%
Ile (I) 27 5.5%
Leu (L) 37 7.6%
Lys (K) 18 3.7%
Met (M) 11 2.2%
Phe (F) 17 3.5%
Pro (P) 20 4.1%
Ser (S) 29 5.9%
Thr (T) 31 6.3%
Trp (W) 6 1.2%
Tyr (Y) 14 2.9%
Val (V) 43 8.8%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 64

Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon C 2356
Hydrogen H 3740
Nitrogen N 658
Oxygen O 723
Sulfur S 15

Formula: C2356H3740N658O723S15
Total number of atoms: 7492

Extinction coefficients:

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in

water.

Ext. coefficient 54110

Abs 0.1% (=1 g/l) 1.015, assuming all pairs of Cys residues form
cystines

Ext. coefficient 53860

Abs 0.1% (=1 g/l) 1.010, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 35.38

This classifies the protein as stable.
Aliphatic index: 87.41

Grand average of hydropathicity (GRAVY): -0.146