of Phaeodactylum tricornutum (PDB ID: 3DMI) that was expressed in E. coli. Original protein was observed by x-ray diffraction at 1.5A resolution. The figure highlights the heme complex with the Fe sphere; the residue above the heme is MET105 and the residue below is HIS63; not shown is the Mg ligand located around the external perimeter. Residues that flank the heme environment, namely HIS and MET, could affect the pyridine hemochrome assays absorbance reading and the extinction coefficient that is observered from analyzing the heme environment.