FOR IDEAL REACTORS GROWTH PATTERNS IN BATCH CULTURE PRODUCT FORMATION KINETICS

A material balance on moles of 1.Growth-associated Product Formation

component i shows that the rate of accumulation
of component i (given by the time derivative of
total amount of concentration i in the reactor)
must be equal to the net rate of formation of
component i due to chemical reaction in the
vessel.
Thus;

( ) =

Where VR = culture volume

ci = molar concentration of component i 2.Non-growth Associated Product Formation

moles i formed by reaction
=
unit culture volumeunit time
Typical growth curve for a bacterial population
IDEAL CSTR Describing Growth Kinetics by Some
Stoichiometrically Related Parameters
Yield coefficients: defined based on the amount of
consumption of another material.

: / =

3.Mixed Mode Product Formation
: / =

Chemostats: A completely mixed continuous
stirred-tank reactor for the cultivation of cells
/2 =
2
In the steady state, where all the
concentrations within the vessel are independent Maintenance Coefficient, m: describe the specific rate
of time, we can write of substrate uptake for cellular maintenance.

[ ]
[ ][ ] =



+[ ]=0

QUANTIFYING GROWTH KINETICS MODELS WITH GROWTH INHIBITORS

Modelling Cell Growth Substrate inhibition: At high substrate concentrations,

The complete description of the growth
kinetics of a culture would involve recognition of
the structured nature of each cell and the
segregation of the culture into individual
units(cells) that may differ from each other.
Structured Models : Model divides cell
mass into components (by molecule or by
element) and predicts how these components
change as a result of growth. These models are
very complex and not used very often.
An unstructured model assumes fixed
cell composition, which is equivalent to
Balanced Growth. Model assumes balanced
growth where cell components do not change
with time. Much less complex and much more
commonly used.
The simplest way to model cell culture
systems will be to consider an unstructured,
unsegregated model. For such a model, the
growth rate expression can be written as (Shuler
and Kargi 1992):
microbial growth rate is inhibited by the substrate.
Product inhibition: High concentrations of product
can be inhibitory for microbial growth.
Inhibition by toxic compounds: uses rate expressions
for competitive, noncompetitive, and uncompetitive
inhibition of growth in analogy to enzyme inhibition.
THE LOGISTIC EQUATION
KINETICS OF SUBSTRATE
UTILIZATION, PRODUCT
FORMATION AND BIOMASS
1 PRODUCTION IN CELL
= (1 )
CULTURES
GROWTH MODELS FOR FILAMENTOUS ORGANISMS ChE 519(E) Biochemical Engineering
Filamentous organisms such as molds often form PREPARED BY:
microbial pellets at high cell densities in suspension Agripa, Cyril Ann
culture. The growth models of molds should include the Aguilar, Sandino Michael Angelo
simultaneous diffusion and consumption of nutrients Uy, John Rhen B.
within the pellet at large pellet sizes. Vibal, Jessica
BS ChE 5
AY: 2017 - 2018

where, rx is the rate of cell generation, X is the cell
concentration and is the specic growth rate.
MONOD EQUATION
CYBERNETIC MODELS
Measurement
Another modeling approach has been
developed primarily to predict growth under conditions
when several substrates are available. These substrates
may be complementary (e.g., carbon or nitrogen) or
substitutable. One approach to modeling growth on
multiple substrates is a cybernetic approach. Cybernetic
means that a process is goal seeking (e.g., maximization
of growth rate).
Ideal Reactors for Kinetics
When a small quantity of living cell is added
to a liquid solution of essential nutrients at a
suitable temperature and pH, the cell will grow.
Cell growth process got two manifestation
Increase in biomass are accompanied by
increases in the number of cells present.
Increase the growth of mold size