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Standard Methods
for Microbiological Analysis
of Drinking Water
organised in co-operation with
1
Laboratory manual for the microbiological analysis
of drinking water: sterilization, sample collection,
microbiological media and storage, laboratory
facilities, equipment, materials, procedures, and
result analysis
2
INDEX
1. Laboratory Manual for Microbiological Analysis
1.1 Sterilization
1.2 Sample collection
1.3 Microbiological media and storage
1.4 Laboratory facilities
1.5 Equipment
1.6 Materials
1.7 Procedures
1.8 Result analysis
1.9 Test Report
2. References
3
1. Laboratory Manual for Microbiological Analysis
4. LABORATORY
1.STERILIZATION 7. PROCEDURES
FACILITIES
2. SAMPLE 8. RESULT
5. EQUIPMENT ANALYSIS
COLLECTION
3.
MICROBIOLOGICAL
6. MATERIALS 9. TEST REPORT
MEDIA AND
STORAGE
4
1.1 Sterilization
5
1.1 Sterilization
EQUIPMENT EQUIPMENT
& MATERIAL & MATERIAL
FOR FOR
SAMPLING ANALYSIS
CONTROLLED
ENVIRONMENT
FOR SAMPLING
& ANALYSIS
6
1.1 Sterilization
7
1.2 Sample
Collection
1.2 Sample Collection
Sampling containers
Sampling procedure
Sample preservation
Sampling Storage
Records
10
1.2 Sample Collection
11
1.2 Sample Collection
Sample number
Code of container
Parameters for analysis
Sampling procedure
Sampling date
12
1.2 Sample Collection
Sample number
Sampling point
Sampling data
Number of containers
13
1.2 Sample Collection
Sampling point
14
1.2 Sample Collection
15
1.2 Sample Collection
1
Sample should only be collected if
the tap presents normal
conditions of higiene and
conservation
16
1.2 Sample Collection
2
Disinfect the tap, using a flame.
17
1.2 Sample Collection
4
Without closing the tap collect the
sample for microbiology (sterile
container)
19
1.2 Sample Collection
5
For the other parameters, wash the
flasks 2 3 times with the tap water
and fill the flasks totally, at a
constant flow (unless stated
otherwise)
20
1.2 Sample Collection
6
If required, measure residual chlorine in the sampling point
Register the
residual chlorine
21
1.2 Sample Collection
22
1.2 Sample Collection
The bottle for MB should not be in contact with the coolers (to
avoid thermal shock of the microorganisms). This bottle should
be put in the middle of the box, together with flask for
temperature measurement.
AT LABORATORY:
24
1.2 Sample Collection
The sample has been delivered after its maximum time for
storage
25
1.3 Microbiological Media and
Storage
26
1.3 Microbiological Media and Storage
ISO 11133:2014 - Microbiology of food, animal feed and
water -- Preparation, production, storage and
performance testing of culture media
27
1.3 Microbiological Media and Storage
ISO 11133:2014
28
1.3 Microbiological Media and Storage
ISO 11133:2014
29
1.3 Microbiological Media and Storage
ISO 11133:2014
30
1.3 Microbiological Media and Storage
ISO 11133:2014
31
1.4 Laboratory
Facilities
32
1.4 Laboratory Facilities
Flow of Personal
Assembly
Material
Cleaning
Waste
Sterilization
HVAC
FACILITY
EQUIPMENT
Open
PERSONNEL MICROBIAL Vs
CONTAMINATION Closed
SOURCES PROCESS
UTILITIES
MATERIALS
Raw material
WATER Reusable resin &
Membrane filter
33
1.4 Laboratory Facilities
34
1.4 Laboratory Facilities
36
1.4 Laboratory Facilities
37
1.5 Equipment
38
1.5 Equipment
41
1.6 Materials
42
1.6 Materials
Sterile Loops
43
1.7 Procedures
44
1.7 Procedures
MICROBIOLOGY LABORATORY SHALL HAVE PROCEDURES
DOCUMENTED FOR:
Water Sampling
ISO 19458:2006, Water quality Sampling for microbiological analysis
Microbiology Analysis
46
1.8 Result Analysis
47
1.8 Result Analysis
The resulting data shall be recorded in such a way that trends are
detectable and, where practicable, statistical techniques shall be
applied to the reviewing of the results.
48
1.8 Result Analysis
49
1.8 Result Analysis
51
1.9 Test Report
b) the name and address of the laboratory, and the location where
the tests were carried out, if different from the address of the
laboratory;
g) the date of receipt of the test where this is critical to the validity
and application of the results;
53
1.9 Test Report
54
1.9 Test Report
k) a statement to the effect that the results relate only to the items
tested, where relevant;
55
2. References
56
2. References
ISO 3696:1987, Water for analytical laboratory use Specification and test
methods
Standard Methods for the Examination of Water and Wastewater 22th edition
58
Laboratory manual for the microbiological analysis of drinking
water: sterilization, sample collection, microbiological media and
storage, laboratory facilities, equipment, materials, procedures, and
result analysis