Tour:

-makerbot : takes design, prints out layer by layer with hot melted liquid thing through hot
extrusion, solid to liquid to solid - cheaper, faster but lower res (100 Microns), can be retrofitting,
halkhead(?),
can print network of cells
LOOK FOR APPLICATIONS TO TISSUE ENGINEERING
heat could potentially damage cells or cause apoptosis
-formlabs: uses photo-sensitive resin, prints upside-down, laser shows to harden, liquid to solid -
create finer structure due to higher resolution (25 microns)

PDMS - silicon-oxygen branch of CH3s (look at first quiz), most widely used material in
microfluids, you can change its hydrophobic/philic properties

PCR -

oxygen plasma treatment both surfaces and theyll stick

****soft lithography:
-negative mold, pour heated PDMS, make a channel, like digging a trench
-used for testing (like showing different temperature)

QUIZ 3:
1 question 3D printing
3 questions stroke, imaging, cell targeting
1 question nerve regeneration

review session 4pm tuesday

duraseal = hydrogel made with PEG(?)/very hydrophilic, not ideal for brain/spinal cord repair,
expands a lot, does some major damage

1) if you can prevnet scar tissue from forming or bridge it

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3) first point goes for stroke as well
a) nanostructures focus*
b) nanostructures can have really nice mech props
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7) have a lot of surface area, neurotrophin*, actually attach growth factors to surface of
tube
a) electrically conductive if trying to recapitulate neural function
8) double bonds have resonance (bond is oscillating, electrons can be delocalized and
moved between multiple atoms, lower energy state)
a) as double bonds move around, electrons move, electricity conducting
 b) biocompatible, support neuronal growth, polypyrroles really cool apparently
9) can also display resonance
10) also electrically conducting by virtue of hexyl(maybe) group
a) nanofibers/tube can be helpful for axon and dnedrite lengthening, mimics
electircity like in nerves
11) peptide = series of amino acids, you can pluck them out for polypeptides
a) laminin natural scaffold material
b) if you make it the right peptide IKVAV in aqueous solution, they self assemble
i) bc of some parts of hydrophibic and philic
ii) cluster together, making a nanofiber as a result
1) advantageous bc amphopathic/philic
12) possibility of destroying structures if implanted too soon, or the small amount of scar
tissue needed will not form (time is not known yet)
a) window of opportunity = once the bleeding is under control but before so much
scar tissue is formed that neurons cannot elongate
13) vague time window, someone with spinal cord injry
a) physical rehab
b) any sort of regain of motor/sensory functionn
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