Florida Fish and Wildlife Conservation Commission

Fish and Wildlife Research Institute

Habitat Selection by Stream Indicator Biota: Development of Biological Tools for the
Implementation of Protective Minimum Flows for Florida Stream Ecosystems

Florida State Wildlife Grant Pro ject No. 98502532195

G.L. Warren and E.J. Nagid, Principal Investigators

Will A. Strong, Travis Tuten, Darrie Hohlt, Eric Nelson and Jennifer Bernatis, Project Biologists
Gainesville Freshwater Fisheries Research Laboratory
7922 NW 71st Street, Gainesville, FL 32653
FW RI Library No. F2195-05-08-F
November 30, 2008
 ABSTRACT

Groundwater and surface water withdrawals associated with development pose a

significant threat to the integrity of stream ecosystems in Florida. The states five water
management districts are responsible for establishing minimum flows and levels (MFLs) that
both protect stream ecosystems and provide adequate water supplies for consumptive users.
Most methods used to establish MFLs have employed hydrological and geological parameters,
and have ignored ecological processes despite the fact that MFLs are intended to protect
ecological integrity. There has been no ecological monitoring to verify that the methods
employed are protective of stream ecosystems. The Florida Fish and Wildlife Conservation
Commission implemented research aimed at eventually providing the water management
districts with alternative methods intended to ensure the sustainability of stream ecosystems.
Fish and invertebrate communities in a ten- mile segment of the Withlacoochee River, Madison
and Hamilton Counties, Florida, were sampled from 2005 to 2008 to determine flow, depth and
habitat relationships. The PHABSIM model was employed to determine usable habitat areas at
varying stream discharges in eight study reaches. Time series analyses of PHABSIM results
combined with a 30 years of stream discharge data were utilized to establish critical flow
periods. Results showed that invertebrate communities of the Withlacoochee River were
dominated by suites of species dependent upon fine particle size detritus and attached algae as a
food resource. Abundances of many dominant invertebrate taxa were influenced by current
velocity and depth. Habitats associated with hydraulic controls (cobble/gravel riffles), or that
were located above the stream bed (snags and tree root systems), were inhabited by the most
species rich, diverse, and flow-dependent invertebrate assemblages. These habitats would be the
first to be de-watered in receding water conditions. Optimum current velocities for most
dominant invertebrate taxa ranged between 30 and 90 cm/sec. Optimum depths ranged between
20 and 140 cm. Results of invertebrate community PHABSIM analysis showed that optimum
habitat areas for most dominant species occurred at discharges ranging from 340 to 1300 cfs.
Results of time series analyses indicated that flow reductions of 30 and 40 percent during the
critical the autumn months of September through November would adversely affect populations
of dominant invertebrate taxa and probably result in long-term alterations to invertebrate
community structure. Juvenile largemouth bass, Suwannee bass and spotted suckers selected
water velocities less than 6 cm/s, while adults of these species and American eels selected water
velocities from 10-24 cm/s. Juveniles of all species selected depths within the range of 30 to 90
cm, whereas adults selected a wider range of depths from 60 to 450 cm. A variety of cover types
were used among juveniles, however organic debris packs was most commonly selected among
black basses, whereas juvenile spotted suckers most commonly selected sand substrates with no
cover or over- hanging vegetation. Adults among all species commonly selected snag and
falldown cover types, with the exception of American eels which most selected undercut bank
and boulder/rock outcroppings. The PHABSIM output estimated the amount of usable area
available for all juvenile target species to decrease as discharge increased, whereas adults
generally obtained optimum usable areas between discharges of 500-700 cfs, followed by
decreasing area as discharge increased. Simulated flow reductions resulted in mostly habitat
gains for the selected fish species, and a 40% reduction in flow was necessary to produce about a
10% habitat loss based on composite reach calculations. Flow reductions could provide
conditions more suitable for largemouth bass than Suwannee bass, but these species generally are
not affected by flow reductions as other fish species may.

i
 ACKNOWLEDGEMENTS

The authors extend their gratitude to James Gore, Ph.D., of the University of South

Florida at St. Petersburg, for his invaluable contributions to this project, which included selection

of Withlacoochee River transects for PHABSIM modeling, construction of habitat suitability

curves, execution of the PHABSIM model for a number of taxa and community quality

descriptors, and time series analyses of PHABSIM results.

The authors also express their gratitude to John Good, Ph.D., of the Suwannee River

Water Management District, who graciously provided an historical flow simulation for the

Withlacoochee River at Lee, Florida, USGS stream flow gauge.

We thank the following individuals from the University of Florida who assisted us with

field efforts: Jason Bennett, Doug Colle, Drew Dutterer, and Lauren Marcinkiewicz.

We thank the following present and former FWC staff for their dedicated efforts in the

field and/or laboratory: Tracey Smith, John Johansen, Cara Miller, Ann Cichra, and Amanda

Waddle.

The authors thank the U.S. Fish and Wildlife Service for providing funding for this work

through the state wildlife grant program, and Brian Branciforte of the FWC Habitat and Species

Conservation Division for his administrative efforts associated with the project.

We also thank Dick Krause and Jim Estes of FWC for their reviews of the initial draft.

ii
 TABLE OF CONTENTS

Abstract ..............................................................................................................................................i

Acknowledgements ......................................................................................................................... ii

Introduction ......................................................................................................................................1

Background ......................................................................................................................................2

Objectives.........................................................................................................................................6

Study Area........................................................................................................................................7

Technical Approach .......................................................................................................................10

Selection of a Habitat Simulation Model ..................................................................................10

PHABSIM Transect Survey Methods.......................................................................................12

Invertebrate Sampling Methods ................................................................................................15

Fisheries Sampling Methods .....................................................................................................18

Development of Fish Habitat Suitability Criteria .....................................................................19

Results ............................................................................................................................................20

Aquatic Invertebrate Community Structure ..............................................................................20

Invertebrate Habitat Suitability Curves ....................................................................................30

Invertebrate Community PHABSIM Analysis .........................................................................37

Times Series Analysis of Invertebrate PHABSIM Results.......................................................37

Fish Habitat Utilization .............................................................................................................47

Fish Habitat Suitability Curves ..................................................................................................59

Fish PHABSIM Analysis ...........................................................................................................92

Time Series Analysis of Fish PHABSIM Results......................................................................97

Discussion ....................................................................................................................................106

Conclusions ..................................................................................................................................112

Appendices...................................................................................................................................120

iii
 Habitat Selection by Stream Indicator Biota: Development of Biological Tools for the
Implementation of Protective Minimum Flows for Florida Stream Ecosystems

INTRODUCTION

The future health of stream ecosystems in Florida is dependent upon responsible

stewardship in the midst of development and increasing water use by a growing human

population. From 1950 to 2005 Floridas population grew from 2.8 to 17.9 million, a 539 %

increase (Smith 2005). During this same period, withdrawals of freshwater from surface and

groundwater sources increased from 1,454 million gallons per day to 6,868 million gallons per

day (372 % increase; Marella 2008). Withdrawals of groundwater have exceeded aquifer

recharge in several areas of Florida, resulting in groundwater level reductions by as much as 80

feet (Berndt et al 1998) and reduced surface water flows (Vergara 1994, Rao and Clapp 1996,

Berndt et al. 1998). Groundwater pumping has been responsible for complete cessation of spring

flows (e.g. Kissengen Spring, Polk Co.; Berndt et al. 1998). As Floridas population continues to

grow and land development progresses, freshwater withdrawals from surface and underground

sources can be expected to increase (Vergara 1994, Rao and Clapp 1996, Berndt et al. 1998), and

competition for the dwindling resource among household users, industry, and agriculture will

make water an even more valuable commodity.

Streams are complex and dynamic systems that are affected by a multitude of physical

and biological variables. Flow and water level are among the most ecologically influential of

these variables (Hynes 1970, Allan 1995). The magnitude, frequency, duration, timing, and rate

of change of flow events that characterize the natural flow regime influence the water quality,

energy resources, physical habitat and biotic interactions that collectively determine the

ecological integrity of lotic systems (Resh et al. 1988, Poff and Ward 1989, Poff et al. 1997,

1
Richter et al. 1997). Alteration of the natural flow regime, such as reduction of flows to levels

chronically below the normal hydrological range, pose a substantial threat to the integrity of

stream ecosystems (Poff and Ward 1989, Richter et al. 1997). Abiotic factors such as dissolved

oxygen, temperature, suspended sediments, bed load, and streambed stability are directly

influenced by flow and, in turn, directly affect the distribution of stream-dwelling organisms

(Ward and Stanford 1983, Allan 1995, Richter et al. 1996). Many stream organisms (e.g. net-

spinning caddisflies) are directly dependent upon specific ranges of flow for survival (Hynes

1970, Statzner et al. 1988, Gore et al. 2001). Physical habitat alterations caused by

anthropogenic stream flow manipulation have been shown to influence the abundance and

species composition of fishes in cold and warm water streams (Schlosser 1985; Bain et al. 1988;

Kinsolving and Bain 1993).

Given the likely future increases in groundwater and surface water withdrawals, and the

potential for ecological damage resulting from these withdrawals, natural resource agencies and

non-government organizations concerned with maintaining healthy stream ecosystems must be

prepared to provide constructive input to the water management districts at the outset of the MFL

establishment process. Insights provided by these additional inputs will help ensure the

formulation of flow recommendations that attain the overall goal of sustaining the ecological

integrity of streams. The present project was implemented as an initial step in this process.

Background

Floridas five water management districts are required by law (Section 373.042 FS) to

establish MFLs for streams and lakes within their respective jurisdictions. The statute defines

minimum flow as the limit at which further withdrawals would be significantly harmful to the

water resources or ecology of the area. The law further states that minimum flows and levels

2
are to be determined using the best available information and are to reflect seasonal variation

when appropriate. The statute provides no specific guidance regarding the terms significantly

harmful and best information available, but State Water Policy (62-40.473 F.A.C.) requires

that the water management districts must consider all of the following factors when determining

MFLs:

Recreation on and in the water

Fish and wildlife habitats and the passage of fish
Estuarine resources
Transfer of detrital material
Maintenance of freshwater storage and supply
Aesthetic and scenic attributes
Filtration and absorption of nutrients and pollutants
Sediment loads
Water quality
Navigation

The overall intent of the legislation and policy directive was to provide adequate water

for consumptive users while ensuring maintenance of flows and levels necessary to protect biota

and safeguard ecological processes within streams, springs, and lakes. However, the concept of

regulating stream withdrawals using a minimum flows approach, which emerged in the

western U.S. states during the 1960s, has been substantially criticized for its inability to provide

for sustainable ecosystem function or habitat protection (Annear et al. 2004, Calow and Petts

1992, 1994). The Florida Fish and Wildlife Conservation Commission (FWC), as the state

agency charged with protecting Floridas fish, wildlife, and habitat resources, undertook the

work presented herein as an initial step in the formulation of biological tools intended to protect

stream-dwelling biota in the MFL process. FWC contributions are intended to support the water

management districts efforts to develop MFLs that are truly protective of aquatic ecosystems

and satisfy the needs of consumptive users.

3
Aquatic Inve rtebrate and Fish Communities as Ecological Indicators

Invertebrates play an integral role in the function of healthy aquatic ecosystems. As

secondary producers invertebrates act as a fulcrum in aquatic food webs by converting the

products of primary production into carbohydrate and protein for export up the food chain.

Invertebrates are a primary food resource to many fish, amphibian, reptile, bird, and mammal

species, as well as to other invertebrates. Invertebrates also play critical roles in the

decomposition and nutrient cycling processes via their feeding and digestive activities. Adverse

impacts to stream invertebrate assemblages can, therefore, have ecosystem-wide ramifications.

In addition to their functional roles, invertebrates serve as barometers of ecosystem

health. Invertebrate community structure has been used as a tool to evaluate the status of

freshwater systems since the origin of the science of limnology in the early 20th century

(Thienemann 1925, Brundin 1949, Brinkurst 1974). Early researchers recognized that

community structure was sensitive to prevailing environmental conditions. Since invertebrate

assemblages are essentially sessile and unable to escape disturbance, they are sculpted by, and

reflect, the habitat conditions in which they develop. Consequently, invertebrate community

structure, and the presence/absence and abundance of certain key invertebrate taxa, function as

an indicator of habitat, and overall ecosystem quality.

The environmental requirements of resident invertebrate taxa play a major role in

structuring invertebrate communities. Bovee et al. (1978) demonstrated that invertebrates have

narrower tolerances of flow change than fish. Many stream-dwelling invertebrate taxa require

flow (as current velocity) for survival. Flowing water supplies food to gathering and filter-

feeding taxa and oxygen to taxa that respire via gills and spiracles. Among those taxa most

dependent upon flows are many species of mayflies (Ephemeroptera), stoneflies (Plecoptera),

4
caddisflies (Trichoptera), and non-biting midges (Chironomidae). Prolonged and chronic

streamflow reductions can severely alter stream communities by stressing or eliminating flow-

dependent taxa. The absence of these taxa can, in turn, affect food webs and ecological

processes ecosystem-wide.

In addition to environmental requirements and tolerances, the elements of invertebrate

community structure used for characterizing invertebrate community quality include: species

composition, species richness, evenness (equitability of distribution of numbers among species

as per Pielou 1977), diversity (as a combined measure of richness and evenness, e.g. Shannons

equation, Krebs 1999), relative abundance, and absolute abundance (no. of organisms per unit

surface area).

Fish communities and individual fish species have also been used as indicators of system

health and as a means of evaluating system disturbance (Karr 1981; Fausch et al. 1984; Miller et

al. 1988). Shifts in the relative abundance of obligate riverine, facultative riverine and

facultative lacustrine species may also provide indications of flow change. As previously

mentioned however, fishes tend to exhibit greater tolerance to flow changes than invertebrates,

particularly in peninsular Florida streams in which obligate riverine species are generally absent.

Thus, the effect of a natural flow deviation on fish populations is related to the sensitivity of

species-specific habitat requirements. It is important to understand habitat utilization relative to

availability by fishes to identify thresholds and predict population and community level

responses. The fish species selected for this study are Suwannee bass Micropterus notius,

largemouth bass M. salmoides, American eel Anguilla rostrata and spotted sucker Minytrema

melanops. The Suwannee bass is a black bass that is generally confined to fluvial systems east

of the Ochlocknee River and west of the Suwannee River drainage. It is an important species for

5
recreational angling and is listed as a State of Florida Species of Special Concern (FAC 68A-

27.005). The largemouth bass, also a black bass, is highly valued by recreational anglers. It is

widely considered a habitat generalist and will serve to potentially elucidate habitat utilization

similarities or differences with Suwannee bass. The American eel is a species with little habitat

utilization information and was chosen due to its catadromous life history. The spotted sucker is

also predominantly a fluvial species often considered sensitive to poor water quality with an

affinity to limerock environments.

OBJECTIVES

Floridas five water management districts use a variety of approaches to determine

minimum flows and levels in their respective districts; however, few of these approaches

consider the biological communities that MFL legislation and policy were designed to include,

due in part to a lack of fish and wildlife information. The overall purpose of the research

presented herein was to begin development of ecology-based MFL information that would focus

upon protection of habitat and biological diversity and, thereby, provide fish and wildlife

guidance to the five water management districts. To achieve this purpose, the following

objectives were developed and addressed:

1. Determine taxonomic composition and abundance of aquatic invertebrate communities in

an appropriate study stream.

2. Develop habitat selection (preference) curves for selected fish and aquatic invertebrate
taxa sampled within the study area.

3. Model the relationships between hydrologic and habitat variables in selected stream
segments.

4. Using results from objectives one, two, and three, predict the usable habitat area for
selected fish and invertebrate species at variable flows and levels using an existing, or
developed, habitat simulation model.

6
 These objectives were selected with the expectation that the ensuing product would

benefit the aquatic resources of Florida by providing support to the states water management

districts in the development of MFLs. The work will also benefit the Florida Comprehensive

Wildlife Strategy by addressing two required elements: (1) Descriptions of locations and relative

condition of key habitats and community types essential to conservation of declining species or

those indicative of ecosystem health, and (2) Descriptions of problems which may adversely

affect those species or their habitats. As such, the information gained is not necessarily limited

to the study stream, and may be transferable to other streams within the subject species

geographical ranges.

STUDY AREA

The study area was the northern Withlacoochee River (Madison and Hamilton Cos.,

Florida) from approximately 1 km upstream (north) of Florida State Route (SR) 6 highway to the

confluence with the Suwannee River near Ellaville, Florida (Figure 1, Table 1). The

Withlacoochee originates in south Georgia near the cities of Tifton and Moultrie and drains

approximately 6,035 km2 (2,330 mi2 ) of mostly agricultural lands in Georgia and Florida (Hand

et al. 1996). Most of the watershed lies in Georgia. The river flows through the Gulf Coastal

Lowlands Physiographic Zone and, in the study area, is underlain by Tertiary period

differentiated strata that include Ocala Limestone, Suwannee Limestone, the Torreya Formation,

the Statenville Formation, and the Miccosukee Formation (Scott et al. 1991). An extensive karst

system links the Florida aquifer to surface waters in the study area. A number of springs, most

prominently Madison County Blue Spring and Morgans Spring, arise along the river and supply

clear water of low dissolved oxygen and constant temperature to the stream. Madison County

Blue Spring is a first magnitude spring (annual median flow > 100 cfs) that discharges to the

7
FIGURE 1. The Withlacoochee River, Florida, study area ( = relative locations of stream
reaches containing transects surveyed for PHABSIM analysis).

8
TABLE 1. Geographic locations of Withlacoochee River, Florida, transects surveyed for
PHABSIM habitat simulation modeling.

River Channel Reach

Latitude Longitude
Segment Morphology Boundary
Reach 1 Shoal NA N 030o 28.6130' W 083o 14.5550'

Reach 2 Shoal Shoal NA N 030o 28.3910' W 083o 14.6320'

Reach 2 Run Run Upstream N 030o 28.1934' W 083o 13.8714'

Pool N 030o 28.1706' W 083o 13.7652'
Run Downstream N 030o 28.1214' W 083o 13.7016'

Reach 3 Shoal Upstream N 030o 28.0914' W 083o 13.5456'

Pool N 030o 28.0404' W 083o 13.5300'
Shoal Downstream N 030o 28.0086' W 083o 13.4610'

Reach 3.5 Shoal Upstream N 030o 26.0004' W 083o 13.2390'

Pool N 030o 25.9440' W 083o 13.2138'
Shoal Downstream N 030o 25.8756' W 083o 13.1760'

Reach 4 Shoal NA N 030o 25.2380' W 083o 13.1290'

Reach 5 Shoal NA N 030o 25.0920' W 083o 12.9090'

Reach 6 Shoal Upstream N 030o 24.8484' W 083o 11.3904'

Shoal N 030o 24.8286' W 083o 11.3652'
Pool N 030o 24.7950' W 083o 11.2704'
Shoal Downstream N 030o 24.7542' W 083o 11.1846'

Reach 7 Run Upstream N 030o 24.2238' W 083o 10.7028'

Pool N 030o 24.1494' W 083o 10.6848'
Run Downstream N 030o 24.0294' W 083o 10.6236'

9
Withlacoochee via a 50 meter spring run near Florida SR6 highway (Figure 1). Madison Blue

Spring supplies up to 20 percent of the total Withlacoochee discharge in the study area during

low- flow periods.

Within the study area the Withlacoochee is characterized by steep sand and limestone

banks and alternating sequences of deep pools, runs, limestone bedrock/cobble/gravel shoals, and

shifting sand shoals. Mean daily Withlacoochee River discharges during the study period ranged

from 245 cfs (1-02-08) to 17,900 cfs (3-02-08); Figure 2). The Withlacoochee crested above

flood stage after the passing of two hurricanes during the summer of 2004; however, the work

described herein was conducted mostly during an extended period of drought.

TECHNICAL APPROACH

Selection of a Habitat Simulation Model

The Physical Habitat Simulation Model (PHABSIM; Milhous et al. 1984, 1989), a

system of models for simulating available stream habitat, was selected as the most appropriate

tool for evaluating potential habitat loss resulting from reduced streamflows in the

Withlacoochee system. PHABSIM is one component of the Instream Flow Incremental

Methodology (IFIM; Milhous et al. 1989, Stalnaker et al. 1995, Bovee et al. 1998), a prioritized

collection of field, modeling, and negotiation protocols intended to assist natural resources

managers and stakeholders in the determination and selection of appropriate stream management

alternatives. PHABSIM integrates hydrological and microhabitat models to produce an output:

weighted usable area (WUA). WUA quantifies the amount of micro-habitat available for a target

organism over a range of stream discharges. PHABSIM is the most commonly used simulation

model for determining potential impacts of reduced flows. It has been used in a variety of stream

types in the western, midwestern, and southeastern U.S.

10
FIGURE 2. Northern Withlacoochee River discharge at the Lee, Florida USGS streamflow
gauge (no. 02319394) during the gauge period of record (Nov. 2000 through Nov. 2008). The
FWC study was executed during the time period between the vertical red lines (1 July 2005 30
June 2008).

11
PHABSIM Transect Survey Methods

Transects were selected and surveyed using PHABSIM protocols as per Bovee (1997)

and Bovee et al. (1998). Study reaches and PHABSIM transects were selected during May 2006

with the assistance of Dr. James Gore of the University of South Florida. A total of 20 transects

were distributed among nine study reaches (Figure 1, Table 1). Transects were selected based

upon their flow characteristics (laminar, one dimensional), streambed morphology, presence of

microhabitats typical of the reach, and relation to instream hydraulic controls (mostly limestone,

bedrock shoals). Transects were marked by placing a permanent headpin at each transect start

point on the left bank, looking upstream, and a tail pin at each transect end on the right bank.

The pins were placed above the normal high water mark to allow location of transects in all

conditions except extreme high flows.

PHABSIM requires a suite of habitat measurements at increments along each transect

during both low flow and moderate flow conditions. The minimum number of measurement

increments (stations) along each transect was determined by dividing the bank-to-bank wetted

width of the river at the location by 20. The resulting quotient was the minimum number of

stations along the transect. Distance between stations never exceeded five feet (1.5 m). To

facilitate measurements a marked Kevlar tag line was placed perpendicular to stream flow at

each transect. The tag line was anchored to trees or augered tie-downs. Head and tail pins were

never used to tie-down the tag line. Habitat measurements were obtained by wading when water

depth was less than 1.5 m and stream flow conditions safely permitted. Measurements were

obtained from a boat using U.S. Geological Survey (USGS) boat equipment in depths greater

than 1.5 m or in high flow conditions. The USGS equipment was designed to stabilize a boat in

swift current. It consisted of an aluminum frame that was bolted to the boat gunnels and through

12
which a steel cable was threaded and affixed to each stream bank. The cable was stretched tight

with a come-along winch to stabilize the boat and allow movement perpendicular to flow.

Habitat measurements obtained at each station along each transect included distance from

headpin (interval- x), height of substrate above ground surface (i.e.; cobble or boulder), substrate

type (Table 2), water depth, and average current velocity. Water column depth was measured

using a 2 m pole calibrated in one centimeter increments when wading, or with a sounding reel

when using the boat. Current velocities were measured using a Marsh-McBirney Flo- mate

2000 portable meter. At depths of 100 cm or less, average current velocity was determined from

a measurement taken at 0.4 x total depth from the stream bottom (60 percent total depth

from surface; Smith and Stopp 1978). At depths exceeding 100 cm, average velocity was

obtained by taking the mean of measurements made at 0.2 and 0.8 x total depth (Smith and Stopp

1978). Bed elevation and water surface elevation were recorded using a total station surveying

instrument. Water surface elevations were measured along each transect at three locations: left

bank waters edge, mid-channel, and right bank waters edge.

For each reach surveyed (whether a single transect or set of transects), a benchmark

was established to enable the linking of all distance and height measurements along the transect

to a common datum. Benchmarks were placed on permanent structures (trees, bedrock ledges)

above the normal high water mark. An instrument height was set at each benchmark to

establish a common datum for stream bed heights. Measurements of intervals, benchmarks, and

water surface elevations were recorded in 10th s of feet. Ground elevations and intervals were

measured to the nearest 0.1 ft. Water surface elevations were measured to the nearest 0.01 ft.

13
TABLE 2. Florida Fish and Wildlife Conservation Commission substrate
and cover type designations used for PHABSIM analysis of selected
northern Withlacoochee River fish and invertebrate taxa.

Cover/substrate Description Code

Bedrock no cover Bedrock substrate with no cover present 1

Sand no cover Sand substrates with no cover present 2

Cobble/gravel 3

Roots/knees Exposed roots of riparian trees and knee 4

projections of cypress trees Taxodium spp.

Snag Large woody debris, typically resting on the 5

river bottom

Organic debris pack Includes fine woody debris and leaf litter 6

Boulder/rock outcrops Bedrock forms of cover including boulders, 7

rock overhangs, rock ledges and rock
crevices

Falldown Trees fallen into stream, typically occupying 8

the majority of the water column, and
composed of many branches (and
occasionally leaves) of different sizes

Overhanging vegetation Terrestrial vegetation hanging over and into 9

the stream

Undercut bank A bank in which the profundal portion has 10

eroded away by the water action

Submersed vegetation Rooted submersed macrophytes 11

Emergent grass Rooted emergent macrophytes along the 12

riparian zone

Floating vegetation / Predominantly water hyacinth Eichhornia 13

algae crassipes and filamentous algae forming
large mats in low flow areas or held by
other instream cover such as falldowns.

Upland vegetation Terrestrial vegetation along the riparian 14

zone

14
 Low- flow measurements were obtained 8 June 6 July 2006, when discharge measured

at the USGS streamflow gauge at Lee, Florida, ranged between 460 cfs and 1,030 cfs. Moderate

flow measurements were obtained 4 February 19 February 2008, when discharges at the Lee

gauge ranged from 1,170 cfs to 1,600 cfs

Invertebrate Sampling Methods

Aquatic invertebrate communities were sampled from ecologically important habitats in

selected stream reaches (Figure 3) using habitat-specific quantitative methods. Habitats sampled

included limestone bedrock outcroppings, shallow cobble/gravel riffles, sand sediments, snags

(wood debris), tree root systems, and organic debris packs. One hundred eighteen samples were

obtained from eight study reaches (Figure 3) during the field component of the study.

Communities associated with limestone bedrock and cobble/gravel riffles were sampled

using a Hess sampler (Jacobi 1978, Merritt et al. 2008). Sand sediments in runs and pools were

sampled using a petite ponar dredge (Powers and Robertson 1967, Merritt et al. 2008). Snags

(wood debris) were sampled using a bag and saw technique developed by Warren et al. (2000).

Tree root systems and benthic organic debris packs were sampled using a quantitative dip-net

technique developed by Warren et al. (2006). All sampling and rinsing devices were fitted with

300 mesh Nitex to enable collection of smaller-sized invertebrates. Upon collection, each

sample was rinsed in a 300 mesh sieve bucket to remove excess water, then preserved

separately in a labeled quart jar with 95% ethanol.

A suite of environmental parameters was measured concurrently with the collection of

each individual sample. Bottom substrate type was visually characterized according to the

modified Wentworth scale. Average water column current velocity and nose depth current

velocity were measured with a Marsh-McBirney Flo-Mate Model 2000 portable meter. Bottom

15
 N
SR 6
Reach 0

Madison Blue Spring

Reach 1

Reach 3

Withlacoochee River

Reach 3.5
Reach 3.75

141
Morgan Spring CR

Reach 6

US 90

0 Kilometers 3 Reach 7

Suwannee River

FIGURE 3. Relative locations of Florida Fish and Wildlife Conservation Commission aquatic
invertebrate sampling sites in the northern Withlacoochee River, Florida, study area, 2005-2007.

16
depth was measured with a meter stick or calibrated wading rod. Water temperature, dissolved

oxygen, and specific conductance were measured using a YSI Model 556 multi-parameter meter

at the surface, bottom, average water column depth and nose depth.

Samples were transported to the Florida Fish and Wildlife Conservation Commission

Freshwater Invertebrate Laboratory in Gainesville for processing. Each sample was processed

separately; samples were not composited. Processing was conducted by trained laboratory

technicians using stereo-dissecting microscopes with magnifications to 43X. Technicians placed

small sample portions in water-filled glass petri plates and then, with the aid of forceps and a

stereo-scope, removed invertebrates. The technicians identified and sorted invertebrates to major

taxonomic groups, enumerated each taxon, and preserved the organisms with 95 percent ethanol

in three-dram vials. A taxonomist then verified counts and identified all organisms in each

sample to the lowest taxonomic level possible, given the age and physical condition of

specimens. To facilitate identification of Oligochaeta, Chironomidae, Ceratopogonidae, and

Simuliidae, specimens of these taxa were slide- mounted using CMC-10 and then identified using

a compound interference-contrast microscope with magnification to 1000X.

A separate laboratory sheet was prepared for each sample. Taxonomic composition and

taxa abundances were recorded on these sheets, along with ancillary measurement data, and

station/date identifiers. Sample site information, ancillary measurements, taxa codes, and raw

organism counts were entered from laboratory sheets into a database maintained in Microsoft

Excel. Taxa richness, diversity (Shannons equation as per Krebs 1999), and evenness (Pielou

1977) were computed for each sample and entered into the database. Descriptive statistics and

univariate analyses were computed using SYSTAT 12 (SYSTAT Software, Inc. 2007) and

JMP 7 (SAS Institute Inc., 2007) statistical software. Multivariate analyses were computed

17
using PC-ORD Version 5 (McCune and Mefford 1999). Habitat preference curves for selected

invertebrate indicator taxa were developed for input into the PHABSIM stream habitat

simulation model by Dr. James Gore of the University of South Florida at St. Petersburg using

Withlacoochee River data and the methods of Gore and Judy (1981). Dr. Gore executed the

PHABSIM model using the selected taxa and computed time series analyses to determine

weighted usable habitat areas available at study transects throughout the entire discharge range

for the period of record at the USGS Pinetta stream gauge on the Withlacoochee River.

Fish Sampling Methods

We used a 4.9 m aluminum electrofishing boat equipped with a Smith-Root 5.0 GPP to

collect juvenile and adult largemouth and Suwannee basses, juvenile and adult spotted suckers,

and American eels within the study area depicted in Figure 1. Because the targeted species tend

to associate with instream structure, 200 m long transects were located parallel to the riparian

zone, where the majority of instream cover existed in the form of overhanging terrestrial

vegetation, tree roots, limerock outcroppings and within-channel snags. Sampling was

performed seasonally in winter (January-March), spring (April-June), summer (July-September),

and fall (October-December) months to account for temporal shifts in habitat utilization.

Transects were predominantly located within run mesohabitats and effort was made to plan

collection trips near baseflow discharges during each season. Thus, river bends and riffle

habitats were generally avoided where water depth and clarity limited the electrofishing

efficiency, and where water velocity was too great to effectively capture stunned fishes,

respectively. Similarly, sampling near base flow conditions minimized stunned- fish drift during

electrofishing and maximized water clarity to more accurately determine the fishs location and

observe the microhabitat characteristics.

18
 Prior to fish sampling, 5 numbered buoys were randomly deployed within each 200 m

transect for available habitat estimation. During fish sampling, a numbered buoy was deployed

over the habitat where each target individual was encountered for utilized habitat estimation.

Buoys were not deployed if the original location of the target species was uncertain. Collected

target fish were measured to total length (millimeters) and returned to the water. Subsequent to

fish sampling, microhabitat measurements were recorded at each buoy corresponding to either

available or utilized habitats. Water velocity (m/s) was recorded at 0.6 of the depth in the water

column using a Marsh-McBirney Flo- mate 2000 portable velocity meter. Water column depth

(cm) was recorded using a 2 m pole in centimeter increments, or a Speedtech Depthmate model

SM-5 if the depth exceeded 2 m. Cover and substrate type were qualitatively visually described

and were assigned to categories (Table 2). Both water velocity and depth were recorded at the

buoy, whereas cover and substrate was described within a 0.91 m radius of the buoy.

Development of Fish Habitat Suitability Criteria

Length frequency histograms were constructed for largemouth and Suwannee basses and

spotted suckers from each sampling event to identify the length break between juvenile and adult

fishes. We assumed that the first mode in the distribution represented juveniles as either age-0 in

the summer and fall collections, or age-1 fishes when collected the winter and spring. All

subsequent lengths following the first mode were considered adults.

The data were separated by sampling event, species, and life stage to examine if habitat

utilization relative to habitat availability within seasons served as reasonable replicates. Habitat

suitability criteria per species were developed using category III criteria methods described by

Bovee (1986). The data were organized into bins of 6 cm/s water velocity intervals, 30 cm depth

intervals, and cover/substrate categories shown in Table 2. The suitability (selection) of velocity,

19
depth and cover to target species was demonstrated by independently calculating the ratio

between the relative frequencies of utilization and availability across the variable intervals using

the following equation:

Si = Ui / Ai,

where Si is an index of suitability at xi, Ui is the relative frequency of fish observations at xi, Ai is

the relative frequency of xi available during the sampling events, and xi is the interval of the

variable x. The suitability index was then standardized to provide a scale from 0 to 1 by dividing

the ratios across the variable intervals by the maximum ratio value. Ratios must be standardized

for use with PHABSIM since the maximum value of any weighting factor used in the program is

one (Bovee 1986). Because the suitability equation is a function of both habitat utilization and

habitat availability, the term selection is often used interchangeably with suitability throughout

the remainder of the fish portion of this document. However, we offer no defining value or

threshold to discern habitat selection verses avoidance. Rather, statements regarding selection or

suitability are quantified within the scale of zero to one, but are primarily used to express the

relative use of a habitat variable over another.

RESULTS

Aquatic Inve rtebrate Community Structure

A total of 207 individual aquatic invertebrate taxa representing 34 major taxonomic

groups were collected from the eight river reaches sampled within the study area (Appendix 1).

The overall river assemblage was numerically predominated by larval non-biting midges

(Diptera:Chironomidae), riffle beetles (Coleoptera:Elimidae), water mites (Acari:Hydrachnida),

caddisflies, (Insecta:Trichoptera), mayflies (Insecta:Ephemeroptera), snails

(Mollusca:Gastropoda) and the Asian clam (Corbiculidae:Corbicula fluminea; Appendix 1).

20
Community structure varied substantially among the micro-habitats sampled (although some taxa

were ubiquitously distributed among all habitats). The most species rich, diverse, and densely

populated communities were present in micro-habitats that were characterized by greater current

velocities and higher levels of dissolved oxygen (bedrock and cobble /gravel riffles), and in

micro- habitats that were located above bottom sediments (snags and tree root systems), but not

necessarily in higher current velocities. Bottom-associated habitats (sand and organic debris

packs) supported the poorest quality communities.

The invertebrate assemblage associated with snags (above-bottom wood debris) was the

most species rich (133 total taxa) and diverse (mean diversity = 3.77) of all communities

sampled (Table 3); however, snags ranked fourth among the six habitats sampled in terms of

mean total organisms per square meter (29,413 m-2 ; Table 4). The snag-associated community

was predominated by the non-biting midges Tanytarsus species C (3,253 m-2 , 11.1%),

Pseudochironomus sp. (3,068 m-2 , 10.4%), and Xestochironomus sp. (1,520 m-2 , 5.2%), the riffle

beetle Stenelmis sp. (2,904 m-2 , 9.9%), and water mites (Hydracarina (1,693 m-2 , 5.8%;

Appendix 2D). These five taxa together accounted for 42.4 percent of all organisms collected

from snags. No other individual taxon accounted for more than 4.4 percent of the total snag

organisms.

The invertebrate community associated with tree root systems was nearly equivalent to

the snag-associated community in terms of total taxa richness, mean taxa richness, and Shannon

diversity, however, taxonomic composition of the two habitats differed substantially. One

hundred twenty-three total taxa were collected from tree roots. Per sample values of species

richness and Shannon diversity averaged 35.0 and 3.77, respectively (Table 3). The non-biting

midge Tanytarsus sp. C was by far the most abundant root-associated taxon (9,324 m-2 ),

21
TABLE 3. Descriptors of invertebrate community quality in ecologically important habitats in
the northern Withlacoochee River, Florida, 2005-2007: total taxa richness, and per sample
means of taxa richness, diversity (Shannons equation), and evenness (as per Pielou 19xx).

Habitat _
Bedrock Cobble Riffle Sand Snags Tree Roots Debris Packs

Total Taxa 90 115 121 133 123 58

Mean Taxa Richness 27.3 38.3 19.9 35.5 35.0 37.0

Mean Diversity 3.07 3.49 2.75 3.77 3.77 2.97

Mean Evenness 0.65 0.67 0.66 0.74 0.76 0.57

22
TABLE 4. Density (no. m-2 ), coefficient of variation (c.v.), and percent composition (%) of key invertebrate indicator taxa collected
from six ecologically important habitats in the northern Withlacoochee River study area, Florida, 2005-2007.

Habitat _
Bedrock _ Cobble Riffle Sand _ Snags _ Tree Roots_ Debris Packs
no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.)
Taxon % % % % % %

Gastropoda (total) 107 (1.62) 835 (1.36) 246 (2.07) 92 (1.75) 1,679 (1.09) 48 (0.35)
0.3 1.5 1.8 0.3 2.8 0.3

Amphipoda (total) 71 (1.60) 173 (1.78) 42 (2.01) 966 (2.61) 5,627 (1.17) 97 (0.69)
0.2 0.3 0.3 3.3 9.4 0.5

Hyalella azteca 33 (1.74) 103 (1.55) 34 (2.22) 903 (2.64) 2,196 (1.80) ---
<0.1 0.2 0.2 3.1 3.7

Aquatic Acari (Hydracarina) 659 (0.71) 5,600 (1.17) 268 (1.66) 1,693 (0.74) 1,341 (1.05) 280 (0.96)
1.7 10.1 2.0 5.8 2.2 1.6

Ephemeroptera (total) 788 (1.19) 3,104 (1.03) 48 (2.45) 1,215 (0.80) 2,596 (1.01) 261 (0.62)
2.0 5.6 0.4 4.1 4.3 1.5

Total Baetidae 176 (1.91) 484 (1.06) 12 (2.70) 170 (1.23) 208 (1.17) 87 (1.73)
0.4 0.9 <0.1 0.6 0.3 0.5

Pseudocloeon ephippiatum 16 (1.81) 128 (2.44) --- 15 (3.77) 29 (3.87) ---

<0.1 0.2 <0.1 <0.1

Total Heptageniidae 2 (3.61) 97 (1.21) 1 (6.24) 397 (1.28) 49 (2.96) 48 (0.35)

<0.1 0.2 <0.1 1.35 <0.1 0.3

23
TABLE 4 (cont.)

Habitat _
Bedrock _ Cobble Riffle Sand _ Snags _ Tree Roots_ Debris Packs
no. m-2 (c.v.) no. m-2 (c.v.) -2
no. m (c.v.) no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.)
Taxon % % % % % %

Megaloptera (hellgrammites)
Corydalus cornutus 5 (2.90) 105 (2.40) --- 7 (2.01) --- 5 (1.73)
<0.1 0.2 <0.1 <0.1

Trichoptera (total) 2,000 (0.94) 2,916 (0.95) 165 (3.18) 3,691 (1.16) 7,542 (0.77) 431 (0.37)
5.1 5.3 1.2 12.5 12.6 2.4

Total Hydropsychidae 323 (1.31) 461 (1.84) 14 (4.30) 968 (1.30) 63 (3.52) 29 (1.31)
0.8 0.8 0.1 3.3 0.1 0.2

Hydropsyche rossi 213 (1.33) 137 (1.70) 1 (6.24) 528 (1.71) 7 (3.87) 5 (1.73)
0.5 0.2 <0.1 1.8 <0.1 <0.1

Total Hydroptilidae 1,386 (1.02) 2,061 (1.01) 74 (3.21) 1,291 (0.90) 4,422 (0.91) 193 (0.96)
3.4 3.7 0.5 4.4 7.4 1.1

Hydroptila sp. 1,192 (1.06) 1,566 (1.12) 49 (3.42) 1,000 (1.15) 1,748 (0.83) 39 (0.87)
3.1 2.8 0.4 3.4 2.9 0.2

Coleoptera (beetles)
Stenelmis sp. (larvae) 4,536 (1.77) 8,783 (2.08) 162 (1.36) 2,904 (0.76) 2,299 (1.24) 358 (0.17)
11.7 15.9 1.2 9.9 3.8 2.0

24
TABLE 4 (cont.)

Habitat _
Bedrock _ Cobble Riffle Sand _ Snags _ Tree Roots_ Debris Packs
no. m-2 (c.v.) no. m-2 (c.v.) -2
no. m (c.v.) no. m-2 (c.v.) no. m-2 (c.v.) no. m-2 (c.v.)
Taxon % % % % % %

Total Chironomidae (midges) 22,137 (0.95) 28,049 (1.05) 6,447 (1.04) 15,395 (0.73) 31,720 (0.57) 15,167 (0.58)
57.0 50.8 47.2 52.3 53.0 85.8

Cricotopus bicinctus 688 (2.44) 853 (1.80) 33 (4.73) 228 (1.92) 4,673 (1.05) 174 (1.45)
1.8 1.5 0.2 0.8 7.8 1.0

Parakiefferiella sp. 2,259 (2.15) 1,131 (0.88) 254 (3.42) 1,281 (2.19) 2,805 (3.87) 522 (0.89)
5.8 2.0 1.9 4.4 4.7 3.0

Tvetenia vitracies 1,229 (2.02) 393 (2.07) 113 (3.99) 983 (2.73) 67 (2.45) 58 (1.73)
3.2 0.7 0.8 3.3 0.1 0.3

Pseudochironomus sp. 7,537 (1.37) 4,965 (1.49) 476 (3.50) 3,068 (1.92) 296 (1.92) 135 (0.89)
19.4 9.0 3.5 10.4 0.5 0.8

Stempellina sp. A 122 (3.61) 2,073 (2.71) 855 (1.78) 72 (2.03) 542 (2.06) 19 (1.73)
0.3 3.8 6.3 0.2 0.9 0.1

Tanytarsus sp. C 3,362 (1.61) 11,942 (1.41) 465 (2.14) 3,252 (1.63) 9,324 (1.32) 232 (1.00)
8.6 21.6 3.4 11.1 15.6 1.3

Total Organisms 38,865 (0.67) 55,241 (0.88) 13,665 (0.84) 29,413 (0.50) 59,802(0.59) 17,686 (0.47)
100.0 100.0 100.0 100.0 100.0 100.0

25
accounting for 15.6 percent of all organisms collected (Appendix 2 E). No other individual taxon

accounted for more than 4.9 percent of the total. Other numerically important root-associated

taxa included the amphipods Gammarus sp. (2,905 m-2 , 4.9%) and Hyalella azteca (2,196 m-2 ,

3.7%), the riffle beetle Stenelmis sp. (2,299 m-2 , 3.8%), and the midge Parakiefferiella sp. (1,870

m-2 , 3.1%; Appendix 2E). Mean total organisms in tree roots (59,802 m-2 ) was the highest of all

habitats sampled (Table 4).

The bedrock and cobble/gravel riffle habitats supported high quality invertebrate

communities that were slightly subordinate to the snag and tree root-associated communities in

terms of taxa richness and diversity. Ninety and 115 total taxa were obtained from the bedrock

and cobble/gravel habitats, respectively (Table 3). Mean taxa per sample averaged 27.3 and 38.3

in bedrock and cobble/gravel, respectively, while Shannon diversity averaged 3.07 and 3.49,

respectively (Table 3). Tanytarsus sp. C, Stenelmis sp., and Pseudochironomus sp. were the

three most abundant taxa in both the bedrock and cobble/gravel habitats. Together these three

taxa accounted for 46.5 percent of all organisms collected from cobble/gravel and 39.7 percent of

the bedrock total (Appendices 2A and 2B).

The sand and organic debris pack-associated invertebrate communities exhibited the

lowest mean diversities (2.75 and 2.97, respectively) and lowest mean total organism densities

(13,665 m-2 and 17,686 m-2 , respectively) of all Withlacoochee River habitats sampled (Tables 3

and 4). Both habitats were numerically predominated by a single taxon that was far more

abundant than any other single organism, thus contributing to the lower mean diversity values.

The Asian clam Corbicula fluminea was the most abundant taxon collected from sand (2,042 m-
2
), accounting for 14.9 percent of the total organisms (Appendix 2C). The lone remaining

organism accounting for greater than four percent of the total sand organisms was the non-biting

26
midge Stempellina sp. A (Appendix 2C). The orthocladine midge Corynoneura sp. B accounted

for 50.2 percent (8,884 m-2 ) of all organisms collected from organic debris packs (Appendix 2F).

Midges of the genus Corynoneura are thought to be pollution-sensitive (Simpson and Bode

1980). The only remaining debris pack-associated taxon that occurred in densities greater than

1,000 m-2 and accounted for more than three percent of the total was the nonbiting midge

Polypedilum sp. (Appendix 2 F).

Functional feeding group analysis (Cummins 1973 and 1974, Hawkins and Sedell 1981,

Merritt et al. 2002) was conducted upon habitat-specific invertebrate assemblages to evaluate the

Withlacoochee River in the study area in terms of the nutritional resource base available and to

provide a baseline for future analyses focused upon potential stream flow reduction impacts and

associated consequences to food resource availability and the food web. Invertebrate taxa were

assigned to functional feeding groups according to the determinations of Barbour et al. (1999)

and Cummins et al. (2008; Table 5).

Results of the analysis indicated that all sampled habitats were numerically dominated by

the gathering and filter feeding functional groups, which, together accounted for between 53.6

percent (tree roots) and 75.6 percent (debris packs) of the total organisms in each habitat (Figure

4). Gatherers were the single most abundant group in every habitat sampled, while filterers were

second- most abundant in all habitats except organic debris packs, where they constituted only

4.0 percent of the total. Both gatherers and filter feeders utilize upon fine particle size (< 1 mm)

organic matter (FPOM; primarily detritus) as a food resource (Cummins et al. 2008). Filterers

are especially important in the context of flow because many (e.g. net spinning caddisflies) rely

upon flow to inflate spun nets and to provide a drifting source of FPOM. Periphyton scrapers

accounted for between 17.1 and 22.5 percent of the total fauna in habitats characterized by

27
TABLE 5. Florida Fish and Wildlife Conservation Commission aquatic invertebrate
functional feeding group designations (based upon and modified from Barbour et al.
1999 and Cummins et al. 2008).

Functional Group Food Resource Characteristics

Shredders live and dead CPOM > 1mm diameter

Scrapers periphyton and biofilm from firm surfaces

Gatherers collect FPOM detritus < 1mm diameter

Filterers filter FPOM < 1mm dia. from water column

Predators capture live prey by engulfing or piercing

Generalists scavengers, omnivores

Parasites internal/external association with other animals

28
 100%

90%

80%

70%

60%
Shredders
Scrapers
50% Gatherers
Filterers
40% Predators
Generalists
30% Parasites

20%

10%

0%
Bedrock Cobble Sand Snags Tree Roots Debris Packs
Habitat

FIGURE 4. Aquatic invertebrate functional feeding group composition within the six Withlacoochee River habitats sampled during
2005 2007.

29
harder substrates (bedrock, cobble, snags, and tree roots), and accounted for less than 6 percent

of the total in the softer sand and debris pack habitats (Figure 4). The predator functional group

was a prominent component in the cobble, sand, and snag-associated communities (12.4, 11.4,

11.9 %, respectively), but accounted for less than 7.5 percent of the total abundance in bedrock,

tree roots, and debris packs. The generalist functional group (primarily omnivores and

scavengers) was prominent only in the organic debris pack habitat, where it was the second-most

abundant group collected and accounted for 13.6 percent of the fauna (Figure 4). Shredders did

not account for more than 3.6 percent of the total organisms in any habitat (Figure 4),

demonstrating the general lack of coarse particulate organic matter (CPOM) in the study area.

Invertebrate Habitat Suitability Curves

Habitat suitability criteria constitute the primary biological input to the PHABSIM

model. The product of PHABSIM, weighted usable habitat area, is a func tion of the suitability

of current velocity, depth, and substrate for a given taxon or community quality descriptor at a

given stream transect/discharge. Suitability curves containing the biological data for input into

PHABSIM can be developed from data derived using a number of methods ranging from best

professional estimates (the Delphi method) to site-specific collection of habitat measurements

(current velocity, depth, and substrate) and target organism abundances across ranges of the

habitat parameters (Bovee 1986, Gore and Judy 1981). To construct the curves, suitability for

the current velocity, depth, and substrate parameters is relativized on a scale ranging from zero to

one, with zero representing the least optimum value and one representing the habitat value at

which populations of the subject taxon/community quality parameter attain optimum levels.

Suitability curve construction using data collected on-site allows the establishment of both a

usable range and an optimum range of a given habitat variable for a given species or descriptor

30
in the study stream (Bovee 1986, Gore 1987, Gore et al. 2001). The authors and users of the

PHABSIM model consider the optimum range of a given variable to be 0.85 and above on the

suitability scale. Therefore, reduction of population levels or community quality values by

greater than 15 percent from the optimal level is considered to be ecologically harmful.

We chose to optimize the accuracy of our suitability curves by utilizing species

abundance and community quality parameter data collected directly from the Withlacoochee

River study area. Current velocity, depth, and substrate type were measured with each individual

invertebrate sample collected. Using these data, habitat suitability curves for selected

Withlacoochee River community quality parameters and invertebrate taxa were developed by Dr.

James Gore of the University of South Florida at St. Petersburg, following the methods of Gore

and Judy (1981). Community quality parameters and individual invertebrate taxa for which

curves were developed were selected based upon criteria that included flow dependence,

abundance across all sampled habitats, and importance as a community quality indicator. The

community quality parameters and taxa for which curves were developed are presented in Table

6. All invertebrate suitability curves produced are contained in Appendix 3. Example habitat

suitability curves for net-spinning caddisfly larvae of the genus Hydropsyche and larvae of the

baetid mayfly Pseudocloeon ephippiatum are presented in Figures 5 and 6. The optimum ranges

of flow and depth for all selected indicator taxa and community quality descriptors are presented

in Table 7.

Hydropsyche spp. and Pseudocloeon ephippiatum are examples of ecologically important

invertebrate taxa that are dependent upon flow for survival. Hydropsyche spp. and other net-

spinning caddisflies of the Families Hydropsychidae, Polycentropodidae, and Philopotamidae

(Appendix 1) require flow to provide food resources and to inflate capture nets (Edington 1968,

31
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

FIGURE 5. Current velocity, depth, and substrate suitability curves for the net-spinning
caddisfly Hydropsyche sp. (Trichoptera:Hydropsychidae) collected in the northern
Withlacoochee River, Florida, 2005-2007.

32
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

FIGURE 6. Current velocity, depth, and substrate suitability curves for the mayfly
Pseudocloeon ephippiatum (Ephemeroptera:Baetidae) collected from the northern
Withlacoochee River, Florida, 2005-2007.

33
TABLE 6. Aquatic invertebrate community descriptors and taxa selected for habitat
suitability curve determination and PHABSIM analysis.
Community Descriptor/Taxon
Taxa Richness
Shannon Diversity
Total Organisms
Hydracarina (water mites)
Total Baetidae (mayflies)
Pseudocloeon ephippiatum (mayfly)
Total Hydropsychidae (net-spinning caddisflies)
Hydropsyche sp. (net-spinning caddisfly)
Hydroptila sp. (micro-caddisfly)
Corydalus cornutus (hellgrammite)
Stenelmis sp. (riffle beetle)
Total Chironomidae (midges)
Cricotopus bicinctus
Parakiefferiella sp. (midge)
Tvetenia vitracies (midge)
Pseudochironomus sp. (midge)
Stempellina sp. A (Epler)
Tanytarsus sp. C (Epler)
34
Criteria
Community Quality Indicator
Community Quality Indicator
Community Quality Indicator
Abundance
Abundance, Flow Indicator
Flow Indicator
Abundance, Flow Indicator
Abundance, Flow Indicator
Abundance, Flow Indicator
Flow Indicator
Abundance, Flow Indicator
Abundance, Some Flow Indicators
Abundance
Abundance
Abundance
Abundance
Abundance
Abundance, Flow Indicator
TABLE 7. Optimum ranges of current velocity and depth for the selected community
quality parameters and indicator invertebrate taxa as determined by habitat suitability
curve development.

Optimum Ranges _
Community Descriptor/Taxon Current Vel. (cm/sec.) Depth (cm)

Taxa Richness 9 - 60 29 - 105

Shannon Diversity 18 - 60 24 - 110
Total Organisms 37 - 79 61 - 140
Hydracarina 20 - 63 31 - 98
Total Baetidae 44 - 86 73 - 133
Pseudocloeon ephippiatum 34 - 58 69 - 122
Total Hydropsychidae 34 - 69 53 - 114
Hydropsyche sp. 37 - 70 61 - 114
Hydroptila sp. 41 - 73 67 - 122
Corydalus cornutus 40 - 60 63 - 107
Stenelmis sp. 0 - 30 12 - 62
Total Chironomidae 46 - 94 75 - 154
Cricotopus bicinctus 72 - 91 60 - 91
Parakiefferiella sp. 79 - 183 76 - 183
Tvetenia vitracies 40 - 70 68 - 116
Pseudochironomus sp. 72 - 82 110 - 139
Stempellina sp. A 0-3 9 - 22
Tanytarsus sp. C 40 - 82 69 - 140

35
Wallace 1975). Pseudocloeon ephippiatum occurs on plants and hard substrates in the swiftest

areas of streams where it grazes algae, diatoms, and biofilm, and depends upon flow to

oxygenate gills (Berner and Pescador 1988 as Baetis ephippiatus). Habitat suitability curves

for Hydropsyche spp. (Figure 5) demonstrate an optimum current velocity range of 37 to 70

cm/sec. and an optimum depth range of 61 to 114 cm. The Hydropsyche substrate suitability plot

demonstrates a preference for the snag habitat (Figure 5). Habitat suitab ility curves for

Pseudocloeon ephippiatum (Figure 6) indicate that a current velocity range of 34 to 58 cm/sec

and a depth range of 69 to 122 cm would support an optimum population level. In contrast to

Hydropsyche, P. ephippiatum exhibited a preference for the cobble/gravel riffle habitat.

Examination of current velocity suitability curves for the community quality descriptors

taxa richness, diversity, and total organisms indicated that a range of 37 to 60 cm/sec would

support optimum values for those parameters (Table 7). Optimum depths for the three

descriptors ranged from 61 to 105 cm (Table 7). Optimum current velocity ranges for individual

taxa ranged from a low of 0 3 cm/sec for the chironomid Stempellina sp. A to a high of 79

183 cm/sec for the chironomid Parakiefferiella sp. Optimum velocities for the flow dependent

mayfly (Baetidae), caddisfly (Hydropsychidae and Hydroptilidae), and hellgrammite (Corydalus

cornutus) taxa were moderate among the individual taxa and ranged from 34 to 86 cm/sec.

Optimum depth ranges for individual taxa ranged from a low of 9 22 cm for Stempellina sp. A

to a high of 110 to 139 cm for the chironomid Pseudochironomus sp. Optimum depths for the

flow dependent mayflies, caddisflies, and hellgrammites again ra nged among the moderate

values, with values ranging from 53 to 133 (Table 7).

36
Invertebrate Community PHABSIM Analysis

The PHABSIM model was used to determine areas of usable habitat (WUAs) for selected

invertebrate indicator taxa and community quality d escriptors (Table 6) at given discharges

within the eight PHABSIM study reaches (Table 1). Graphic representations of PHABSIM

results for all taxa and descriptors are presented in Appendix 4. Optimum flows (those that

produced the greatest usable habitat areas) for mean (per sample) taxa richness ranged from 135

cfs at Reach 6 to 650 cfs at Reach 1 (Table 8). Optimum community diversity was attained at

flows ranging from 280 cfs at Reach 5 to 650 cfs at Reaches 1 and 2 (Table 8). The greatest

number of total organisms per sample occurred at discharges ranging from 700 cfs at Reach 7 to

2,000 cfs at Reach 5 (Table 8). Optimum flows for individual taxa ranged from a low of 135 cfs

for both the riffle beetle Stenelmis sp and the midge Stempellina sp. A at Reach 5 to a high 2,500

cfs for several taxa at several transects (Table 8).

Time Series Analysis of Invertebrate PHABSIM Results

To conduct time series analyses that would utilize Withlacoochee River streamflow data

encompassing the longest possible time period, we regressed mean monthly discharges at the

Lee, Florida, streamflow gage (period of record = Nov. 2000 - present) against mean monthly

discharges measured at the Pinetta, Florida gage (period of record = Oct. 1931 - present) for the

Lee gage period of record (R2 =0.95, p<0.05). The Lee gage is located within the Withlacoochee

study area between Reaches 6 and 7. Using the regression equation, monthly values for the Lee

gage were then computed from October 1931 to the beginning of the Lee gage period of record.

Results of time series analyses showed that, in general, flow reductions during the typical

higher flow months of January through April produce increases in habitat area (WUA) at all

study reaches (Tables 9 - 11, Appendix 5). However, flow reductions during the months of May

37
TABLE 8. Discharge (Q, in cubic feet per second [cfs]) at which optimum weighted usable area (WUA, in sq. ft. of habitat/1000 linear feet of
stream) is attained for community quality descriptors and individual indicator invertebrate taxa at the seven PHABSIM study reaches in the
northern Withlacoochee River, Florida.

Stream Reach _
Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7
Q Q Q Q Q Q Q Q
Taxon (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA)

Taxa Richness 650 600 400 280 340 300 135 220
(124,863) (100,743) (40,467) (29,627) (96,474) (88,002) (31,531) (79,985)

Shannon Diversity 650 650 400 400 340 280 400 400
(131,785) (98,278) (37,714) (24,099) (102,721) (94,517) (26,657) (71,657)

Total Organisms 1,200 1,000 950 1,100 1,500 2,000 900 700
(89,595) (82,157) (30,165) (10,159) (68,851) (82,287) (12,462) (36,517)

Hydracarina 650 525 400 170 340 250 400 240

(48,362) (66,423) (28,324) (6,891) (29,626) (57,826) (4,197) (13,602)

Total Baetidae 1,300 1,100 1,150 1,500 2,000 750 1,200 950
(51,742) (62,169) (24,152) (2,247) (34,005) (46,344) (4,992) (12,988)

Pseudocloeon ephippiatum 650 575 650 900 1,050 340 700 575
(124,863) (41,825) (19,221) (854) (15,680) (28,129) (1,463) (3,219)

Total Hydropsychidae 1,200 900 750 475 1,200 340 700 340
(34,891) (31,942) (8,314) (927) (23,055) (14,602) (2,904) (8,974)

38
TABLE 8 (cont.).

Stream Reach _
Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7
Q Q Q Q Q Q Q Q
Taxon (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA)

Hydropsyche sp. 1,150 1,050 950 200 1,200 650 750 340
(35,132) (27,298) (5,903) (799) (24,549) (11,370) (3,355) (9,292)

Hydroptila sp. 1,100 950 900 1,150 1,300 700 900 750
(74,734) (63,163) (22,281) (2,955) (53,194) (47,293) (8,230) (21,685)

Corydalus cornutus 750 525 650 900 950 340 650 575
(18,404) (39,032) (16,812) (610) (9,387) (25,260) (368) (988)

Stenelmis sp. 220 475 250 135 135 200 135 160
(55,825) (50,888) (25,956) (9,384) (39,353) (64,447) (9,386) (16,389)

Total Chironomidae 1,500 1,300 1,300 1,200 2,000 800 1,300 950
(97,750) (86,267) (30,575) (12,659) (68,961) (63,709) (13,907) (44,665)

Cricotopus bicinctus 2,500 2,500 2,500 2,500 2,500 2,500 2,500 2,500
(18,432) (18,768) (11,688) (12,353) (16,179) (28,932) (6,663) (11,204)

Parakiefferiella sp. 2,500 2,500 2,500 2,500 2,500 2,500 2,500 2,000
(81,509) (59,451) (19,203) (10,166) (69,824) (72,432) (16,084) (39,460)

Tvetenia vitracies 1,150 1,200 1,000 1,050 1,300 1,150 850 700
(63,065) (33,040) (8,214) (2,156) (43,982) (25,090) (7,966) (21,424)

39
TABLE 8 (cont.).

Stream Reach _
Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7
Q Q Q Q Q Q Q Q
Taxon (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA) (WUA)

Pseudochironomus sp. 1,500 1,500 1,500 2,000 2,500 2,500 1,300 1,300
(67,711) (30,106) (13,423) (1,781) (34,459) (43,177) (7,682) (25,738)

Stempellina sp. A 190 200 135 135 135 190 135 250
(23,216) (10,165) (7,186) (7,841) (2,784) (7,639) (3,571) (7,454)

Tanytarsus sp. C 1,300 1,050 1,100 2,500 2,000 2,500 1,150 850
(47,789) (63,848) (26,120) (5,132) (34,058) (70,278) (4,823) (12,997)

40
TABLE 9. Time series analysis of PHABSIM results: months of habitat loss exceeding 15 percent at 20 percent flow reductions in the
northern Withlacoochee River, Florida, PHABSIM study reaches.

Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Taxa Richness --- --- --- --- --- --- --- ---

Shannon Diversity --- --- --- --- --- --- --- ---

Total Organisms --- Oct-Nov --- --- --- --- --- ---

Hydracarina --- --- --- --- --- --- --- ---

Total Baetidae --- Oct-Nov --- --- --- --- --- ---

Pseudocloeon ephippiatum --- --- --- Oct-Nov --- --- --- ---

Total Hydropsychidae --- Oct-Nov --- --- --- --- --- ---

Hydropsyche spp. --- Oct-Dec --- --- --- --- --- ---

Hydroptila sp. --- --- --- --- --- --- --- ---

Corydalus cornutus --- --- --- Sep-Jan --- --- --- ---

Stenelmis sp. --- --- --- --- --- --- --- ---

Total Chironomidae Nov Oct-Nov --- --- --- --- --- ---

Cricotopus bicinctus --- --- --- --- --- --- --- ---

41
TABLE 9 (cont.).
Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Parakiefferiella sp. --- Aug-Nov --- --- --- --- --- ---

Tvetenia vitracies Oct-Nov Oct-Nov --- --- --- --- --- ---

Pseudochironomus sp. Jun-Dec Jun-Dec Jun-Dec Jun-Nov Jun-Dec Jun-Nov Aug-Nov Aug-Dec

Stempellina sp. A --- --- --- --- --- --- --- ---

Tanytarsus sp. C --- --- --- --- --- --- --- ---

--- = no months with flow reductions 20 percent.

42
TABLE 10. Time series analysis of PHABSIM results: months of habitat loss exceeding 15 percent at 30 percent flow reductions in
the northern Withlacoochee River, Florida, PHABSIM study reaches.

Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Taxa Richness --- --- --- --- --- --- --- ---

Shannon Diversity --- --- --- --- --- --- --- ---

Total Organisms Oct-Nov Oct-Nov --- --- --- --- --- ---

Hydracarina --- --- --- --- --- --- --- ---

Total Baetidae Sep-Dec Oct-Nov Oct-Nov Oct-Nov --- --- Oct-Nov Nov

Pseudocloeon ephippiatum Nov --- --- Sep-Dec --- --- --- ---

Total Hydropsychidae Oct-Nov Oct-Nov --- --- --- --- --- ---

Hydropsyche spp. Oct-Nov Oct-Dec --- --- --- --- --- ---

Hydroptila sp. Oct-Nov --- --- Oct-Nov --- --- --- ---

Corydalus cornutus Nov --- --- Aug-Jan --- --- Nov ---

Stenelmis sp. --- --- --- --- --- --- --- ---

Total Chironomidae Jun-Dec Oct-Nov Oct-Nov Oct-Nov --- --- Nov ---

Cricotopus bicinctus Jan-Apr --- --- --- --- --- --- ---

43
TABLE 10 (cont.).
Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Parakiefferiella sp. Sep-Nov Aug-Nov Nov --- --- --- Nov Nov

Tvetenia vitracies Sep-Dec Oct-Nov Oct-Nov --- --- --- Nov ---

Pseudochironomus sp. May-Dec Jun-Dec Jun-Dec Jun-Dec Jun-Dec May- Dec Jun-Dec Jun-Dec

Stempellina sp. A --- --- --- --- --- --- --- ---

Tanytarsus sp. C Oct-Nov --- --- Sep-Nov --- --- --- ---

--- = no months with flow reductions 30 percent.

44
TABLE 11. Time series analysis of PHABSIM results: months of habitat loss exceeding 15 percent at 40 percent flow reductions in
the northern Withlacoochee River, Florida, PHABSIM study reaches.

Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Taxa Richness --- --- --- --- --- --- --- ---

Shannon Diversity --- --- --- --- --- --- --- ---

Total Organisms SepDec Oct-Nov Oct-Nov Nov --- --- Nov ---

Hydracarina --- --- --- --- --- --- --- ---

Total Baetidae Sep-Dec Oct-Dec Sep-Dec Sep-Dec Nov --- Sep-Dec Oct-Dec

Pseudocloeon ephippiatum Oct-Nov --- --- Sep-Dec --- --- --- ---

Total Hydropsychidae Sep-Dec Oct-Dec Nov --- --- --- --- ---

Hydropsyche spp. Sep-Dec Sep-Dec Oct-Dec --- --- --- --- ---

Hydroptila sp. Sep-Dec Oct-Dec Oct-Dec Sep-Nov --- --- Oct-Dec ---

Corydalus cornutus Nov --- Nov-Dec May-Dec --- --- Oct-Dec ---

Stenelmis sp. --- --- --- --- --- --- --- ---

Total Chironomidae Jun-Dec Aug-Dec Sep-Dec Sep-Dec Nov --- Sep-Dec Nov-Dec

Cricotopus bicinctus Dec-Aug Sep-Nov Jun-Sep --- --- --- --- Sep&Nov

45
TABLE 11 (cont.).
Location _
Descriptor/Indicator Taxon Reach 1 Reach 2 Reach 3 Reach 3.5 Reach 4 Reach 5 Reach 6 Reach 7

Parakiefferiella sp. Jun-Dec May-Dec Jun-Nov Aug-Nov Jun-Nov Jun-Nov Aug-Nov Sep-Nov

Tvetenia vitracies Sep-Dec Aug-Dec Sep-Dec Nov --- --- Oct-Dec Nov

Pseudochironomus sp. May-Dec May-Dec May-Dec May-Dec May-Jan May-Dec May-Dec May-Dec

Stempellina sp. A --- --- --- --- --- --- --- ---

Tanytarsus sp. C Sep-Nov Oct-Nov Oct-Nov Jun-Dec --- --- Oct-Nov ---

--- = no months with flow reductions 40 percent.

46
through December, historically the lower flow months, produce loss of habitat area for some

indicator taxa at some transects. Some flow reductions result in losses of habitat area that exceed

the critical 15 percent level for some taxa (Tables 9 - 11, Appendix 5).

The time series analysis for the net-spinning caddisfly Hydropsyche sp. at study Reach 1

is characteristic of the typical Withlacoochee River pattern (Figure 7). Flow reductions of up to

40 percent during January through May result in gains of habitat area exceeding 80 percent

(February, Table 8). Conversely, flow reductions of up to 40 percent in the autumn months

result in habitat losses exceeding 30 percent in October and November.

Time series analysis for the chironomid Pseudochironomus sp. at Shoal 5 illustrates the

most extreme impacts of flow reduction in the summer and autumn months (Figure 8). Habitat

area at Shoal 5 increases with flow reductions during the winter months of February and March;

however, beginning in May, flow reductions of up to 40 percent result in decreases in the area of

habitat usable by Pseudochironomus sp. by as much as 64 percent (Figure 8).

It is important to note that flow reductions of as much as 40 percent did not result in

significant habitat area losses ( 15 percent) for the community quality descriptors Shannon

diversity and mean taxa richness at any study reach (Tables 9 - 11, Appendix 5).

Fish habitat utilization

We sampled 122 transects during 8 seasonal sampling events, ranging from 12 to 20

transects per event (Table 12). A fish sample was not collected in the fall of 2006. Over the

course of the study, we made 603 available habitat measurements, and collected 766 largemouth

bass, 647 Suwannee bass, 514 spotted suckers and 108 American eels. Microhabitat utilization

sampling points collected for spotted suckers and black basses were segregated according to

juvenile or adult life stage based on length frequencies within each sampling event (Table 12).

47
 100%

80%

60%
Habitat Gain/Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

0%

-20%

-40%
April

August
July
February
January

December
September
June

October
May

November
March

FIGURE 7. Time series comparison of flow reduction effects upon usable habitat area for Hydropsyche sp.
(Trichoptera:Hydropsychidae) at Withlacoochee River Shoal 1

48
 20%

10%

0%

-10%
Habitat Gain / Loss

10% Flow Reduction

-20%
20% Flow Reduction
30% Flow Reduction
-30%
40% Flow Reduction

-40%

-50%

-60%

-70%
April

August
July

December
January

February

September
June

November
May

October
March

FIGURE 8. Times series comparison of flow reduction effects upon usable habitat area for Pseudochironomus sp.
(Diptera:Chironomidae) at Withlacoochee River Reach 5.

49
Table 12. The number of electrofishing transects, the number of available microhabitat sampling
points, and the number of utilized microhabitat (MH) sampling points (per life stage) during each
seasonal collection on the Withlacoochee River, FL. AMEE = American eel, LMB = largemouth
bass, SUBA = Suwannee bass, and SPSK = spotted sucker.

Winter Spring Summer Fall

2006 2007 2006 2007 2006 2007 2005 2007 Totals
No. of Transects 16 15 16 15 13 15 20 12 122
Available MH points 79 73 80 74 65 75 97 60 603
AMEE MH points 3 2 7 11 29 17 28 11 108
LMB juvenile MH points 2 107 8 26 120 72 0 29 364
LMB adult MH points 22 37 54 63 31 72 18 105 402
SUBA juvenile MH points 0 28 0 49 39 21 4 14 155
SUBA adults MH points 44 15 86 51 45 94 88 69 492
SPSK juvenile points 0 22 0 75 77 0 0 0 174
SPSK adult MH points 38 59 61 41 13 47 54 27 340

50
The 2005 year class of largemouth bass was absent relative to the 2006 year class, represented by

0 juveniles in fall 2005, 2 juveniles in winter 2006 and 8 juveniles in spring 2006. Similarly, the

2005 year classes of Suwannee bass and spotted suckers were absent with 4, 0 and 0 juveniles

and 0, 0 and 0 juveniles collected respectively, in the fall 2005, winter 2006 and spring 2006

sampling events. It also appeared that the 2007 year class of spotted suckers was absent with no

juveniles caught in the summer or fall 2007 sampling events.

The relative frequencies of utilized and available velocities, depths and cover/substrate

types by sampling event are shown in Figures 9-29. Over 50% of available velocities among all

sampling events were less than 6 cm/s, and ranged from 0 to 65 cm/s. In general, the frequencies

of utilized velocities of all species and life stages showed similar trends to the frequencies of

available velocities, suggesting no major disproportional utilization of velocity relative to its

environmental availability (Figures 9-15). However, the adult stages of all species utilized a

greater range of velocities than juvenile stages.

By contrast, there were obvious disproportional uses of depth relative to depth

availability, principally among the juvenile stages of species. Juvenile black basses and spotted

51
 Juvenile largemouth bass
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 9. Relative frequencies of water velocities available and utilized by juvenile largemouth
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

52
 Adult largemouth bass
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 10. Relative frequencies of water velocities available and utilized by adult largemouth
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

53
 Juvenile Suwannee bass
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 11. Relative frequencies of water velocities available and utilized by juvenile Suwannee
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

54
 Adult Suwannee bass
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 12. Relative frequencies of water velocities available and utilized by adult Suwannee
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

55
 Juvenile spotted suckers
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 13. Relative frequencies of water velocities available and utilized by juvenile spotted
suckers by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

56
 Adult spotted suckers
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 14. Relative frequencies of water velocities available and utilized by adult spotted
suckers by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

57
 American eels
Winter 2006 Winter 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring 2006 Spring 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Relative frequency

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer 2006 Summer 2007

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall 2005 Fall 2007

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 15. Relative frequencies of water velocities available and utilized by American eels by
sampling event. Available velocities are represented by broken lines, and utilized velocities are
represented by solid lines.

58
suckers generally utilized shallower depths than adult stages (Figures 16, 18, 20), whereas adult

stages including American eels utilized deeper habitats as well as a greater range of depths

(Figures 17, 19, 21, 22). Available cover types among all sampling events were generally

dominated by boulder and rock outcroppings, snags, tree roots/cypress knees and organic debris

(Figures 23-29), (Refer to Table 2 for cover codes). Floating vegetation and algal mats were a

relatively abundant habitat in the summer and fall of 2007. Fish use of cover varied among

sampling events, but in general there were also disproportional uses of cover relative to available

cover types. American eels utilized boulder and limerock outcropp ings in greater frequency than

other cover types across all sampling events (Figure 29). Juvenile and adult stages of black

basses and spotted suckers always utilized snag and boulder habitats, however, juvenile fishes

utilized sand habitats with no cover and organic debris more frequently than adults, whereas

adults utilized fallen trees in higher frequencies (Figures 23-28).

Fish Habitat Suitability Curves

As a result of the missing 2005 year classes of black basses and spotted suckers, and

small sample sizes among several seasonal sampling events, the construction of habitat

suitability curves (HSC) within seasons to serve as statistical replicates was not possible.

However, since general similarities in relative use of the environmental variables within seasons

were present, data were pooled within seasons to derive one seasonal suitability curve per

variable, and per species life stage. In general, water velocities less than 30 cm/s were suitable

for all juvenile black basses and spotted suckers across all seasons, but typically selected

59
 Juvenile largemouth bass
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 16. Relative frequencies of water depths available and utilized by juvenile largemouth
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

60
 Adult largemouth bass
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 17. Relative frequencies of water depths available and utilized by adult largemouth bass
by sampling event. Available velocities are represented by broken lines, and utilized velocities
are represented by solid lines.

61
 Juvenile Suwannee bass
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 18. Relative frequencies of water depths available and utilized by juvenile Suwannee
bass by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

62
 Adult Suwannee bass
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 19. Relative frequencies of water depths available and utilized by adult Suwannee bass
by sampling event. Available velocities are represented by broken lines, and utilized velocities
are represented by solid lines.

63
 Juvenile spotted suckers
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 20. Relative frequencies of water depths available and utilized by juvenile spotted
suckers by sampling event. Available velocities are represented by broken lines, and utilized
velocities are represented by solid lines.

64
 Adult spotted suckers
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 21. Relative frequencies of water depths available and utilized by adult spotted suckers
by sampling event. Available velocities are represented by broken lines, and utilized velocities
are represented by solid lines.

65
 American eels
Winter 2006 Winter 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring 2006 Spring 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
Relative frequency

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer 2006 Summer 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall 2005 Fall 2007

0.60 0.60

0.40 0.40

0.20 0.20

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 22. Relative frequencies of water depths available and utilized by American eels by
sampling event. Available velocities are represented by broken lines, and utilized velocities are
represented by solid lines.

66
 Juvenile largemouth bass
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 23. Relative frequencies of cover/substrates available and utilized by juvenile largemouth
bass by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

67
 Adult largemouth bass
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 24. Relative frequencies of cover/substrates available and utilized by adult largemouth
bass by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

68
 Juvenile Suwannee bass
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 25. Relative frequencies of cover/substrates available and utilized by juvenile Suwannee
bass by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

69
 Adult Suwannee bass
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 26. Relative frequencies of cover/substrates available and utilized by adult Suwannee
bass by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

70
 Juvenile spotted suckers
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 27. Relative frequencies of cover/substrates available and utilized by juvenile spotted
suckers by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

71
 Adult spotted suckers
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 28. Relative frequencies of cover/substrates available and utilized by adult spotted
suckers by sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

72
 American eels
Winter 2006 Winter 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring 2006 Spring 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00
Relative frequency

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Sum m er 2006 Sum m er 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall 2005 Fall 2007

0.75 0.75

0.50 0.50

0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 29. Relative frequencies of cover/substrates available and utilized by American eels by
sampling event. Available cover/substrate is represented by broken bars. Utilized
cover/substrate is represented by solid bars. Codes are listed in Table 2.

73
water velocities less than 6 cm/s (Figuress 30-32). Water velocities less than 30 cm/s were also

suitable for adult black basses and spotted suckers, but they tended to select water velocities

from 12 to 24 cm/s. Both juvenile and adult Suwannee bass selected relatively greater water

velocities than juvenile and adult largemouth bass, respectively, within the same seasons. The

water velocity suitability for American eels was also generally less than 30 cm/s, however, the

selection varied among seasons (Figure 33).

Juvenile fishes showed similar trends for depth, selecting shallower habitats across

seasons (Figures 34-36). Depths less than 240 cm were suitable for juvenile black basses and

spotted suckers, but they tended to select depths from 30 to 90 cm. By contrast, adult black

basses, adult spotted suckers and American eels selected a wider range of depths within and

among seasons, with overall suitability ranging from 60 to 450 cm (Figures 34-37). Within

black basses, juvenile largemouth bass selected greater depths in all seasons than juvenile

Suwannee bass, whereas no discernable trends were observed among the adults.

The selection of cover types among juvenile fishes was different. In the winter, juvenile

largemouth bass selected emergent grasses, juvenile Suwannee bass selected organic debris, and

juvenile spotted suckers selected overhanging vegetation (Figures 38-40), (Refer to Table 2 for

cover codes). In the spring, juvenile largemouth bass mostly selected falldowns, juvenile

Suwannee bass selected cobble/gravel habitats, and juvenile spotted suckers selected sandy flats

with no cover. In the summer, juvenile black basses selected similar habitats, primarily using

organic debris, floating vegetation/algae, and snag habitats, while spotted suckers continued to

select sandy substrates with no cover. In the fall, juvenile black basses again selected floating

vegetation/algae and organic

74
 Juvenile largemouth bass Adult largemouth bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84
Suitability Ratio

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 30. Water velocity suitability curves by season for juvenile and adult largemouth bass.

75
 Juvenile Suwannee bass Adult Suwannee bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84
Suitability Ratio

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 31. Water velocity suitability curves by season for juvenile and adult Suwannee bass.

76
 Juvenile spotted suckers Adult spotted suckers
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 32. Water velocity suitability curves by season for juvenile and adult spotted suckers. No
juveniles were collected in the fall seasons.

77
 American eels
Winter

1.00
0.75
0.50
0.25
0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spring

1.00
0.75

0.50
0.25
0.00
Suitability Ratio

6 12 18 24 30 36 42 48 54 60 66 72 78 84

Summer

1.00
0.75
0.50
0.25
0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84

Fall

1.00
0.75
0.50
0.25
0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 33. Water velocity suitability curves by season for American eels.

78
 Juvenile largemouth bass Adult largemouth bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 34. Depth suitability curves by season for juvenile and adult largemouth bass.

79
 Juvenile Suwannee bass Adult Suwannee bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 35. Depth suitability curves by season for juvenile and adult Suwannee bass.

80
 Juvenile spotted suckers Adult spotted suckers
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510
Suitability Ratio

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 36. Depth suitability curves by season for juvenile and adult spotted suckers. No
juveniles were collected in the fall seasons.

81
 American eels
Winter

1.00
0.75
0.50
0.25
0.00
30 90 150 210 270 330 390 450 510

Spring

1.00
0.75

0.50
0.25
0.00
Suitability Ratio

30 90 150 210 270 330 390 450 510

Summer

1.00
0.75
0.50
0.25
0.00
30 90 150 210 270 330 390 450 510

Fall

1.00
0.75
0.50
0.25
0.00
30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 37. Depth suitability curves by season for American eels.

82
 Juvenile largemouth bass Adult largemouth bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Substrate/cover code

Figure 38. Cover/substrate suitability plots by season for juvenile and adult largemouth bass.
Codes are listed in Table 2.

83
 Juvenile Suwannee bass Adult Suwannee bass
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Substrate/cover code

Figure 39. Cover/substrate suitability plots by season for juvenile and adult Suwannee bass.
Codes are listed in Table 2.

84
 Juvenile spotted suckers Adult spotted suckers
Winter Winter

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25

0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring Spring

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Summer Summer

1.00 1.00

0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall Fall

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Substrate/cover code

Figure 40. Cover/substrate suitability plots by season fo r juvenile and adult spotted suckers.
Codes are listed in Table 2.

85
 American eels
Winter

1.00
0.75
0.50
0.25
0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spring

1.00
0.75

0.50
0.25
0.00
Suitability Ratio

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Summer

1.00
0.75
0.50
0.25
0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14

Fall

1.00
0.75
0.50
0.25
0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14

Substrate/cover code

Figure 41. Substrate/cover suitability plots by season for American eels. Codes are listed in
Table 2.

86
debris, but Suwannee bass also selected undercut banks. Although not highly seasonally

selected, snags and roots/knees were always seasonally suitable and therefore similarly important

habitats.

Among adult black basses and spotted suckers, falldown and snag habitats were highly

selected in most cases, and utilized among all seasons with the exception of the summer by

spotted suckers (Figures 38-40). When available in the spring and fall, floating vegetation/algae

was also a suitable habitat with a high selection value. American eels highly selected

boulder/rock outcroppings in the spring and summer, bedrock with no cover in winter and

undercut banks in the fall (Figure 41). Falldowns were also highly selected by American eels in

the spring.

Overall, the seasonal suitability trends for velocity and depth by juvenile and adult black

basses and spotted suckers appeared similar. However, the seasonal cover/substrate suitabilities

were more variable, as were the trends for velocity, depth and cover by American eels. Despite

this fact, the seasonal suitabilities were combined to derive one suitability curve per variable per

species life stage for habitat simulation purposes. To accomplish this task, the ratios between

the relative frequencies of utilization and availability were summed across the seasonal variable

intervals, and divided by the maximum ratio to standardize the data from a scale of zero to one.

This approach was sensible considering the utilized and available sample sizes among seasons

for each species life stage were relatively similar. Therefore, the pooled dataset was not biased

towards a particular season. Additionally, the simulation of usable area of each species by

season is unreasonable from a management perspective in an unregulated river. These pooled

habitat suitability curves were the final criteria that were used in the PHABSIM analysis.

87
 The pooled HSCs demonstrated that largemouth bass juveniles selected water velocities

less than 6 cm/s, while water velocities between 6 to 18 cm/s were moderately selected (Figure

42). Water velocities greater than 24 cm/s were not selected. Adult largemouth bass selected

water velocities from 0 to 24 cm/s, and selection decreased beyond 36 cm/s (Figure 42). Depth

selection by juvenile largemouth bass was limited to less than 120 cm, with an optimum

suitability depth of 60 cm (Figure 43). Depths greater than 120 cm were relatively not selected.

Adult largemouth bass utilized the range of available measured depths, and ranged from 30 to

500 cm, but depths in the range of 120 to 240 cm were mostly selected (Figure 43). The three

most selected cover/substrate types by juvenile largemouth bass were organic debris, floating

vegetation/algae and snag habitats (Figure 44). Adult largemouth bass typically selected

falldown, snag and organic debris habitats.

Suwannee bass juveniles most selected water velocities less than 6 cm/s, where suitability

values declined to zero beyond 24 cm/s (Figure 42). Adult Suwannee bass increasingly selected

velocities from 0 to 24 cm/s, where suitability declined beyond velocities above 36 cm/s. Depth

selection by juvenile Suwannee bass was highest below 90 cm, and selection decreased beyond

120 cm, while adults selected a wide range of depths peaking at 180 cm and then decreased to

420 cm (Figure 43). Juvenile Suwannee bass selected organic debris, roots/knees, undercut

banks, snags and floating vegetation/algae habitats, whereas adults selected falldowns,

boulder/rock outcrops and snag habitats (Figure 44).

As with juvenile black basses, juvenile spotted suckers most selected water velocities less

than 6 cm/s, where the suitability declined to zero beyond 36 cm/s (Figure 42). Adult spotted

suckers increasingly selected velocities from 0 to 30 cm/s, and velocity suitability declined

abruptly beyond 30 cm/s. Juvenile spotted suckers most selected depths between 60 and 120 cm,

88
 Largemouth bass juveniles Largemouth bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Suwannee bass juveniles Suwannee bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

Spotted sucker juveniles Spotted sucker adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84 6 12 18 24 30 36 42 48 54 60 66 72 78 84

American eels

1.00
0.75
0.50
0.25
0.00
6 12 18 24 30 36 42 48 54 60 66 72 78 84

Velocity (cm/s)

Figure 42. Pooled velocity (cm/s) habitat suitability curves across all collection dates and
seasons on the Withlacoochee River, FL.

89
 Largemouth bass juveniles Largemouth bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Suwannee bass juveniles Suwannee bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

Spotted sucker juveniles Spotted sucker adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
30 90 150 210 270 330 390 450 510 30 90 150 210 270 330 390 450 510

American eels

1.00
0.75
0.50
0.25
0.00
30 90 150 210 270 330 390 450 510

Depth (cm)

Figure 43. Pooled depth (cm) habitat suitability curves across all collection dates and seasons on
the Withlacoochee River, FL.

90
 Largemouth bass juveniles Largemouth bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Suwannee bass juveniles Suwannee bass adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
Suitability Ratio

1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Spotted sucker juveniles Spotted sucker adults

1.00 1.00
0.75 0.75
0.50 0.50
0.25 0.25
0.00 0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 2 3 4 5 6 7 8 9 10 11 12 13 14

American eels

1.00
0.75
0.50
0.25
0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14

Cover/substrate code

Figure 44. Pooled cover/substrate habitat suitability plots across all collection dates and seasons
on the Withlacoochee River, FL.

91
whereas adult spotted suckers used a wide range of depths under 390 cm, with a suitability peak

between 180 to 240 cm (Figure 43). Juvenile spotted suckers selected over- hanging vegetation

and sand cover/substrate types whereas adults selected falldown, snag and sand cover/substrate

types (Figure 44).

American eels selected water velocities from 0 to 30 cm/s, and did not select velocities

greater than 36 cm/s (Figure 42). Eels utilized a wide range in depths (Figure 43), and

predominantly selected undercut banks, boulder/rock outcroppings, and root/knee cover types

(Figure 44).

Fish PHABSIM Analysis

The output of the PHABSIM model provided estimates of the amount of usable area at

each of the habitat reaches for the targeted species over a range of discharges, based on the

habitat suitability criteria and the habitat reach transects. WUA estimates at each discharge were

summed across each habitat reach to provide a composite WUA estimate that was representative

of shoal and run mesohabitats within the study area for each life stage of the targeted species.

All WUA estimates for each habitat reach are available by life stage and species in Appendix 6.

In general, the physical area available for adult black basses and spotted suckers were

greater than their juvenile life stages at a given discharge at each of the habitat transects sampled.

The range of usable area for juvenile largemouth bass was 9,700 ft2 /1000 linear ft to 47,000

ft2 /1000 linear ft across the simulated discharges (Figure 45). Maximum usable area for juvenile

largemouth bass was available at low discharges (135 cfs), and then steadily declined as

discharge increased. Conversely, the WUA for adult largemouth bass increased from low to

intermediate discharges reaching a maximum of 62,600 ft2 /1000 linear ft at 475 cfs, followed by

92
 Largemouth bass

70000
WUA (sq ft / 1000 linear ft)

60000

50000

40000 Adults
30000 Juveniles

20000

10000

0
135

160

200

250

300

350

400

525

700

950

1200
Discharge (cfs)

Figure 45. Composite weighted usable area per incremental discharge available to juvenile and
adult stages of largemouth bass in the study area of the Withlacoochee River, FL.

93
declining usable areas as discharges increased (Figure 45). Estimates of usable area for adult

largemouth bass ranged from 35,700 ft2 /1000 linear ft to 62,600 ft2 /1000 linear ft.

Juvenile Suwannee bass displayed similar trends to juvenile largemouth bass in usable

area, with the model predicting higher values of usable area at low discharges and declining as

discharges increased. However, the slope for juvenile Suwannee bass was less precipitous than

the curve for juvenile largemouth bass, and the predicted usable area for juvenile Suwannee bass

was greater across all discharge values in comparison to juvenile largemouth bass. Usable area

for juvenile Suwannee bass ranged from 23,100 ft2 /1000 linear ft to 56,000 ft2 /1000 linear ft and

was more than double at high discharge values compared to juvenile largemouth bass (Figure

46).

Adult Suwannee bass likewise showed trends in usable area that were similar to adult

largemouth bass. The usable area increased from low to intermediate discharges, and then

decreased at high discharges (Figure 46). However, the WUA estimates were greater than

estimates predicted for largemouth bass across all discharges. Additionally, the discharge in

which the asymptote was observed was greater for adult Suwannee bass, providing 127,500

ft2 /1000 linear ft at 600 cfs.

The trend in usable area for juvenile spotted suckers was again similar to those observed

in juvenile black basses. Usable area was generally maximized at low discharges and declined

steadily as discharge increased (Figure 47). Usable area predictions ranged from 6,100 ft 2 /1000

linear ft to 39,000 ft2 /1000 linear ft, which were the lowest estimates among the target species

and life stages.

Usable area predictions for adult spotted suckers were the highest among the target

species and life stages. Usable area values ranged from 100,000 ft2 /1000 linear ft to 202,000

94
 Suwannee bass

140000
WUA (sq ft / 1000 linear ft)

120000

100000

80000 Adults
60000 Juveniles

40000

20000

0
135

160

200

250

300

350

400

525

700

950

1200
Discharge (cfs)

Figure 46. Composite weighted usable area per incremental discharge available to juvenile and
adult stages of Suwannee bass in the study area of the Withlacoochee River, FL.

95
 Spotted suckers

250000
WUA (sq ft / 1000 linear ft)

200000

150000
Adults
Juveniles
100000

50000

0
135

160

200

250

300

350

400

525

700

950

1200
Discharge (cfs)

Figure 47. Composite weighted usable area per incremental discharge available to juvenile and
adult stages of spotted suckers in the study area of the Withlacoochee River, FL.

96
ft2 /1000 linear ft (Figure 47). The usable area was predicted to be lowest at low discharges and

increasing to maximum usable area at 700 cfs, followed by declines in usable area when

discharge exceeds 700 cfs.

The usable area predictions for American eels were highly variable across habitat reaches

and no discernable trends were observed. However, the summed estimates pr edicted the WUA

to trend similar to the adult stages of the other target species. Estimates were greatest and

increased from low discharges to a maximum of 99,300 ft2 /1000 linear ft at 340 cfs, and then

declined thereafter at higher discharges (Figure 48).

Time Series Analysis of Fish PHABSIM Results

The graphical results of the time-series analyses across all habitat reaches for each

species and life stage are in Appendix 7. As with the WUA estimates, calculated values of

habitat gains/losses pertaining to percent reductions in discharge were summed across habitat

reaches to derive a composite assessment of habitat gains or losses in the study area representing

shoal and run mesohabitats. Largemouth bass juveniles are predicted to gain habitat across all

months in both time periods as discharge is reduced 10% to 40% from existing conditions

(Figure 49). Largemouth bass adults also show habitat gains across all months with increasing

percent reductions of discharge (Figure 50). Greatest habitat gains are observed in March and

April, whereas relatively lower gains are predicted in October through December.

Suwannee bass juveniles showed habitat gains across all months with reductions in

discharge from 10 to 40% (Figure 51). In comparison to juvenile largemouth bass, Suwannee

bass are expected to receive relatively less habitat gains as discharge is reduced. Adults similarly

showed habitat gains across nearly all percent reductions of discharge (Figure 52). Habitat loss

(-2%) was only observed at 40% reduction in discharge in November during the

97
 American eels

120000
WUA (sq ft / 1000 linear ft)

100000

80000

60000 Adults

40000

20000

0
135

160

200

250

300

350

400

525

700

950

1200
Discharge (cfs)

Figure 48. Composite weighted usable area per incremental discharge available to adult stages
of American eels in the study area of the Withlacoochee River, FL.

98
 Largemouth bass juveniles
1940-1969

80%
Habitat gains / losses

70%
60%
50% 10% reduction
20% reduction
40%
30% reduction
30% 40% reduction
20%
10%
0%

November
June
January

August
March

July
February

September

December
April
May

October

Largemouth bass juveniles

1970-1999

80%
Habitat gains / losses

70%
60%
50% 10% reduction
20% reduction
40%
30% reduction
30% 40% reduction
20%
10%
0%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 49. Composite time-series analysis for juvenile largemouth bass of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

99
 Largemouth bass adults
1940-1969

60%
Habitat gains / losses

50%

40% 10% reduction

20% reduction
30%
30% reduction
20% 40% reduction

10%

0%

November
June
January

August
March

July
February

September

December
April
May

October

Largemouth bass adults

1970-1999

60%
Habitat gains / losses

50%

40% 10% reduction

20% reduction
30%
30% reduction
20% 40% reduction

10%

0%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 50. Composite time-series analysis for adult largemouth bass of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

100
 Suwannee bass juveniles
1940-1969

50%
Habitat gains / losses

40%
10% reduction
30%
20% reduction
30% reduction
20%
40% reduction
10%

0%

November
June
January

August
March

July
February

September

December
April
May

October

Suwannee bass juveniles

1970-1999

50%
Habitat gains / losses

40%
10% reduction
30%
20% reduction
30% reduction
20%
40% reduction
10%

0%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 51. Composite time-series analysis for juvenile Suwannee bass of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

101
 Suwannee bass adults
1940-1969

45%
40%
Habitat gains / losses

35%
30% 10% reduction
25%
20% reduction
20%
30% reduction
15%
10% 40% reduction
5%
0%
-5%

November
June
January

August
March

July
February

September

December
April
May

October

Suwannee bass adults

1970-1999

45%
40%
Habitat gains / losses

35%
30% 10% reduction
25%
20% reduction
20%
30% reduction
15%
10% 40% reduction
5%
0%
-5%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 52. Composite time-series analysis for adult Suwannee bass of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

102
 Spotted suckers juveniles
1940-1969

140%
Habitat gains / losses

120%
100%
10% reduction
80% 20% reduction
60% 30% reduction
40% reduction
40%
20%
0%

November
June
January

August
March

July
February

September

December
April
May

October

Spotted suckers juveniles

1970-1999

140%
Habitat gains / losses

120%
100%
10% reduction
80% 20% reduction
60% 30% reduction
40% reduction
40%
20%
0%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 53. Composite time-series analysis for juvenile spotted suckers of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

103
 Spotted suckers adults
1940-1969

60%
Habitat gains / losses

50%
40%
10% reduction
30% 20% reduction
20% 30% reduction
40% reduction
10%
0%
-10%

November
June
January

August
March

July
February

September

December
April
May

October

Spotted suckers adults

1970-1999

60%
Habitat gains / losses

50%
40%
10% reduction
30% 20% reduction
20% 30% reduction
40% reduction
10%
0%
-10%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 54. Composite time-series analysis for adult spotted suckers of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

104
 American eel adults
1940-1969

45%
40%
Habitat gains / losses

35%
30% 10% reduction
25% 20% reduction
20% 30% reduction
15% 40% reduction
10%
5%
0%

November
June
January

August
March

July
February

September

December
April
May

October

American eel adults

1970-1999

45%
40%
Habitat gains / losses

35%
30% 10% reduction
25% 20% reduction
20% 30% reduction
15% 40% reduction
10%
5%
0%
November
June
January

August
March

July
February

September

December
April
May

October

Figure 55. Composite time-series analysis for adult American eels of habitat gains/losses
associated with 10-40% reductions in monthly discharge.

105
1940-1969 period, and in October (1%) and November (1%) during the 1970-1999 period.

Greatest habitat gain was observed in March and April, while least habitat change was observed

in October and November.

Similar to juvenile stages of black basses, juvenile spotted suckers showed habitat gains

across all levels of discharge reductions during both time periods (Figure 53). Greatest habitat

gains are predicted during March and April, while lesser gains are shown during October and

November. Adult stages showed a peak in habitat gains across all percent reductions during

March and April with relatively minor gains in other months (Figure 54). Habitat loss was

predicted only above 20% reduction in discharges during both time periods during the fall

months, with the maximum habitat loss (8%) occurring at during the 1940-1969 simulation.

The results for American eels were similar to those of the other adult stages. Habitat

gains were predicted over all percentages of discharge reductions, with maximum gains

occurring during March and April and least habitat change occurring during the fall months

(Figure 55).

Discussion

It is important that biological tools become an integral component of the process for

establishing minimum flows and levels in Florida streams. Most methods currently utilized for

formulating MFL recommendations assume that adequately addressing flow requirements

established by measurement of pertinent hydrological and geological parameters will sufficiently

provide for the protection of aquatic biota and ecological processes, and ensure ample water

supply for consumptive users. While current methods give the appearance of being adequate in

their applications to-date, it is logical to assume that ecological tools would be most suitable for

ensuring the protection of ecological integrity. Ecological approaches are needed to fine-tune

106
current MFL methodology to the unique biotic attributes of individual stream systems. As the

analyses presented herein demonstrate, each individual stream system can have unique

ecological indicator species and functional guilds that, in turn, have specific streamflow and

water level needs. Setting MFLs based solely upon hydrological and geological assumptions can

result in unmet ecological requirements of species critical to stream ecosystem function and

integrity.

Results of our analyses show that fine particle size organic matter (FPOM) and attached

algae drive the food chain in the Withlacoochee River study area. The gathering, filter ing, and

scraping invertebrate taxa that depend upon these food resources accounted for from 68 to 85

percent of the total invertebrate organisms collected from the six sampled habitat types. Many of

these gathering, filtering, and scraping taxa are important food web components and/or are

integral to ecosystem metabolism processes. In general, these taxa are most abundant in the

snag, cobble/gravel riffle, and tree root system habitats and they require flow to supply their food

(e.g. net-spinning caddisflies) and/or to oxygenate their gills (e.g. baetid mayflies). Many of

these taxa also have long life spans (1 year or longer) and, hence, require that their habitats be

inundated for long time periods. The snags and root systems where many of these taxa reach

their greatest abundances are located above the stream bottom, and, consequently, are among the

first habitats to be dewatered under receding water level conditions. Given the ecological

importance of the snag and tree root-dwelling fishes and invertebrates, it is critical that above-

bottom habitats be given special consideration in the MFL determination process.

The cobble/gravel riffle habitat is also ecologically critical because of its elevation above

other bottom habitats and because of its structural complexity (and consequent habitat

complexity). Cobble/gravel riffles in the Withlacoochee are located upon the bedrock shoals that

107
serve as hydraulic streamflow controls. Flow reductions and dewatering of this habitat for

prolonged periods of time will result in reduced available habitat area and profound structural

changes to the flow-dependent resident invertebrate community.

The flow (current velocity) needs of many invertebrate taxa are quite specific, as

demonstrated by the current velocity habitat suitability curves and weighted usable habitat areas

presented in Appendices 3 and 4. The Withlacoochee River invertebrate taxa found to be most

responsive to flows were the nonbiting midges Parakiefferiella sp. and Pseudochironomus sp.

Other taxa that were substantially affected by flow included: total Chironomidae, the individual

chironomid species Cricotopus bicinctus and Tvetenia vitracies, total Baetidae (Ephemeroptera),

total Hydropsychidae (Trichoptera), Hydroptila sp. (Trichoptera), and the hellgrammite

Corydalus cornutus. We believe this suite of nine invertebrate taxa to be the most suitable

biological indicator of flow response in the Withlacoochee system. Monitoring of this suite in

the water- level sensitive snag, cobble/gravel riffle, and tree root system habitats will provide a

valuable tool for the evaluation of flow and water level reduction impacts. The community

quality descriptors total taxa, Shannon diversity, and total organisms did not exhibit strong flow

responses, and, along with Hydracarina, Stenelmis sp., Stempellina sp. A, and Tanytarsus sp. C,

are not suitable indicators of flow response in the Withlacoochee system.

We believe that the PHABSIM simulation model and time series analysis of PHABSIM

results provide a useful tool for evaluating the potential ecological consequences of flow and

water level reductions in many Florida stream systems. Our invertebrate community PHABSIM

results indicate that maintaining adequate flow throughout the autumn months of September

through November is critical to sustaining populations of ecologically important invertebrate

taxa in the Withlacoochee River study area (Tables 11 - 13, Appendix 5). We demonstrated that

108
flow reductions of 30 to 40 percent from the monthly mean during the autumn months would

adversely affect populations of dominant invertebrate indicator taxa and would probably result in

long-term negative impacts to invertebrate community structure within the study area. To

maintain optimal habitat area and population levels of the ecologically important net-spinning

caddisfly taxa (e.g. total Hydropsychidae and Hydropsyche sp.), minimum discharges of 340 cfs

are required. To maintain optimal habitat area and population levels of the more flow sensitive

chironomid taxa (e.g. Pseudochironomus sp. and Parakiefferiella sp.), discharges exceeding

1300 cfs are required.

Conversely, the fish species selected for this study were not overly sensitive to stream

flow reductions based on results of the WUA and time-series analyses simulations. In fact,

reductions in stream flow mostly led to habitat gains in both juvenile and adult stages of the

target species examined based on the time-series analysis. However, several trends emerged

from the results that were life-stage specific rather than species-specific. Juvenile black basses

and spotted suckers all selected slow water velocities and shallow depths. Conversely, water

velocity and depth selection among adults tended to be more normally distributed, reaching

maximum suitabilities at intermediate velocities and depths, while extreme ends of the habitat

variables were relatively less suitable. This trend was logical since the drag associated with

current velocity is known to influence fish endurance, foraging success (Schae fer et al. 1999) and

swimming performance (Deegan et al. 2005), where current velocity occupancy is generally

related to physical body size. Size-specific habitat use has also been commonly documented in

many stream fishes due to reasons associated with competition (Freeman and Stouder 1989) and

predation avoidance (Power 1987, Schlosser 1987). Consequently, the amount of usable area

available to juveniles in the study area of the Withlacoochee River decreased as discharge

109
increased, whereas adults generally obtained optimum usable area between discharges of 500-

700 cfs, followed by decreasing area as discharge increased. Examination of the mean monthly

discharge at the Lee gage indicated that most flows were typically greater than 1,100 cfs.

Therefore existing conditions more reflect the trends and values of the WUA estimates at the

extreme end of the discharge simulations.

Despite commonalities among species and life stages, differences within species were

also noted. Juvenile and adult Suwannee bass selected relatively greater water velocities than

juvenile and adult largemouth bass, respectively. Conversely, juvenile largemouth bass selected

greater depths than juvenile Suwannee bass. Cover/substrate types were commonly selected

among adult basses, but boulder/rock outcropping habitats were more suitable to Suwannee bass

than largemouth bass. Thus, WUA estimates were higher for Suwannee bass than for

largemouth bass across all discharges, and suggest that Suwannee bass may indeed possess a

greater affinity to flow. Although, the time-series analysis revealed that black basses would

generally not be negatively impacted by reductions in stream flow based on the composite

calculations, comparisons among juveniles suggests less habitat gains by Suwannee bass than by

largemouth bass, and greater potential habitat losses by Suwannee bass adults at 40% reduction

in stream flows than for largemouth bass adults. Exaggeration of this simulation could

potentially result in the conversion of habitats more suitable for largemouth bass than for

Suwannee bass with increased flow reductions, causing an ecological shift in the community

composition.

Based on the collective examinations of the WUA and time-series for each fish species

and life stage, flow recommendations are difficult. The directive from the Florida Statue to the

five water management districts is to set levels below which significant harm may occur.

110
Several Districts have used 15% as a benchmark above which habitat loss is considered

significant, and below which habitat loss is considered acceptable. Under all composite time-

series presented, no more than 10% habitat is lost even under a 40% reduction in flow from

existing conditions. Thus, based on these criteria for significant harm, this reduction in stream

flow may be acceptable to the fish species selected. A more conservative approach may be to

examine each of the habitat reaches individually and inspect the habitat and passage losses that

are predicted to occur with flow reductions. Under this scenario, Reach 1 (referred to shoal 1 in

Appendix 7) exhibited the greatest loss of habitat to Suwannee bass adults, predicting losses in 6

months out of the year under 40% flow reductions, with a maximum of 26% loss in November

during the 1940-1969 time period, and a maximum 21% loss in October and November during

the 1970-1999 time period. Habitat losses were also expressed in these months from 10% to

30% reductions in stream flow, albeit to a lesser degree, and not exceeding 15% habitat loss.

Adult stages of largemouth bass and spotted suckers similarly exhibit habitat loss in Reach 1 that

exceeded 15% during the fall months. Because Reach 1 is the most upstream habitat transect

sampled, management of water consumptive uses according to discharge s at Reach 1 would

likely provide the most protective flows and levels management to the target fish species in all

downstream habitats of the Withlacoochee River. However, those fishes not encompassed by the

habitat suitabilities of the target species described within may not be fully protected under this

scenario, and care should be taken to ensure passage of fishes such as the anadromous gulf

sturgeon Acipenser oxyrhynchus desotoi is adequate under these flow recommendations.

The fact that these fish species were not overly sensitive to stream flow reductions may

be in part a function of selecting run and pool-dwelling species for PHABSIM analysis, as

opposed to species that occupy shoal or riffle habitats. Since shoal and riffle habitats are the first

111
to be de-watered with reduced flows, fish species that typically occupy these habitats would

likely show the greatest losses in usable habitat. Peninsular Floridas fish fauna generally lack

obligate riverine species, and are more composed of facultative riverine and lacustrine species

that utilize more general and ubiquitous habitats. Another function that may have led to a bias

towards selection of slow velocities and shallow depths was the fish sampling approach. Over

50% of the available velocities among all sampling events were less than 6 cm/s. Although fish

collections were made seasonally, specific effort was made to collect fishes during low flow

conditions, which is contrary to recommendations made by Bovee (1986) in which sampling

fishes over a range of discharges is advised. Because the intention of this study was for MFL

purposes, we emphasized the habitat selection during low flow conditions, and felt sampling the

target fish species during median and high discharge events was both unnecessary and unfeasible

for microhabitat assessments.

Conclusions

Results of our community- level and PHABSIM analyses suggest that streamflow

reductions beyond the range of natural seasonal variation could significantly impact the

ecological integrity of the Withlacoochee system. Principal conclusions from this research were:

1. Withlacoochee River invertebrate communities were dominated by gathering, filtering,

and scraping taxa that are dependent upon fine particle sized detritus and attached

algae/biofilm as food resources. Many numerically dominant invertebrate taxa were

shown to be flow-dependent and were most abundant in the hydraulic control

cobble/gravel riffle habitat and in the above-bottom snag and tree root system habitats.

Snag and tree root systems were also important habitats selected by the target fish

species. Monitoring and protection of these habitats is critical since they harbor sessile,

112
 long- lived, ecologically important communities and are the first habitats to be de-watered

in receding water conditions.

2. Optimum habitat areas for most dominant and/or flow sensitive invertebrate taxa are

available at discharges ranging from 340 to 1300 cfs. 5. Autumn months are critical for

invertebrate taxa and the most vulnerable months for fish species.

3. Flow reductions of 30 to 40 percent from the monthly mean during the autumn months of

September through November would adversely affect populations of numerically

dominant invertebrate taxa and would probably result in long-term alterations of

invertebrate community structure.

4. The targeted fish species for PHABSIM modeling do not appear to be suitable candidates

for flow recommendations in the Withlacoochee River. Flow reductions resulted in

mostly habitat gains for the selected fish species, and a 40% red uction in flow was

necessary to produce about a 10% habitat loss based on composite reach calculations.

5. Flow reductions could provide conditions more suitable for largemouth bass than

Suwannee bass, and invertebrate composition change due to flow reduction would likely

result in fish community change composed of more facultative lacustrine species.

113
 LITERATURE CITED

ALLAN, J.D. 1995. Stream ecology: structure and function of running waters. Kluwer
Academic Publishers. Dordrecht, the Netherlands. 388 pp.

ANNEAR T., I. CHISHOLM, H. BEECHER, A. LOCKE, and 12 other authors. 2004. Instream
flows for riverine resource stewardship, revised edition. Instream Flow Council,
Cheyenne, WY. 268 pp.

BAIN, M.B., J.T. FINN, and H.E. BOOKE. 1988. Streamflow regulation and fish community
structure. Ecology 69:382-392.

BARBOUR, M.T., J. GERRITSEN, B.D. SNYDER, and J.B. STRIBLING. 1999. Rapid
bioassessment protocols for use in streams and wadeable rivers: periphyton, benthic
macroinvertebrates, and fish, second edition. EPA 841-B-99-002. U.S. Environmental
Protection Agency; Office of Water; Washington, D.C.

BERNDT, M.P., E.T. OAKSFORD, G.L. MAHON, and W. SCHMIDT. 1998. Groundwater. In
E.A. Fernald and E.D. Purdum (eds.). Water Resources Atlas of Florida. Institute of
Science and Public Affairs, Florida State University. 312 pp.

BERNER, L., AND M.L. PESCADOR. 1988. The mayflies of Florida, revised edition.
University Presses of Florida, Tallahassee and Gainesville. 415pp.

BOVEE, K.D. 1986. Development and evaluation of habitat suitability criteria for use in the
instream flow incremental methodology. Instream Flow Information Paper 21. U.S. Fish
and Wildlife Service Biological Report 86(7). 235 pp.

BOVEE, K.D. 1997. Data collection procedures for the physical habitat simulation system.
U.S. Geological Survey, BRD, Mid-Continent Science Center, Ft. Collins, CO. 146 pp.

BOVEE, K.D., J.A. GORE, AND A.J. SILVERMAN. 1978. Field-testing and adaptation of a
methodology to measure instream values in the Tongue River, Northern Great Plains
(NGP) Region. U.S. Environmental Protection Agency, EPA-908/4-78-004A. 465 pp.

BOVEE, K.D., B.L. LAMB, J.M. BARTHOLOW, C.B. STALNAKER, J. TAYLOR, and J.
HENRIKSEN. 1998. Stream habitat analysis using the instream flow incremental
methodology. U.S. Geological Survey, BRD Information and Technology Report
USGS/BRD-1998-0004. viii + 131 pp.

BRINKHURST, R.O. 1974. The benthos of lakes. Macmillan Press, London.

BRUNDIN, L. 1949. Chironomiden und andere Bodentiere der sudschwedischen

Urgebirgsseen. Ein Beitrag zur Kenntnis der bodenfaunustischen Charakterzuge
schwedischer oligotropher Seen. Report of the Institute of Freshwater Research,
Drottningsholm 30:1-914.

114
CALOW, P., and G.E. PETTS. 1992. The rivers handbook: hydrological and ecological
principals. Vol. 1. Blackwell Scientific Publications, Boston, MA. 526 pp.

CALOW, P., and G.E. PETTS. 1994. The rivers handbook: hydrological and ecological
principals. Vol. 2. Blackwell Scientific Publications, Boston, MA. 523 pp.

CUMMINS, K.W. 1973. Trophic relations of aquatic insects. Annual Review of Entomology
18:183-206.

CUMMINS, K.W. 1974. Structure and function of stream ecosystems. Bioscience 24:631-641.

CUMMINS, K.W., R.W. MERRITT, and M.B. BERG. 2008. Ecology and distribution of
aquatic insects. In R.W. Merritt, K.W. Cummins, and M.B. Berg (eds.). An introduction
to the aquatic insects of North America, fourth edition. Kendall/Hunt Publishing Co.,
Dubuque, IA. xvi + 39 color plates + 1158 pp.

DEEGAN, L.A., H.E. GOLDEN, J. HARRISON, and K. KRACKO. 2005. Swimming

performance and metabolism of 0+ year Thymallus arcticus. Journal of Fish Biology 67:
910918.

EDINGTON, J.M. 1968. Habitat preferences in net-spinning caddis larvae with special
reference to the influence of water velocity. Journal of Animal Ecology 37:675-692.

FAUSCH K. D., J. R. KARR, AND P. R. YANT. 1984. Regional application of an index of

biotic integrity based on stream fish communities. Transactions of the American Fisheries
Society. 113:3955.

FREEMAN, M.C. and D.J. STOUDER. 1989. Intraspecific interactions influence size specific
depth distribution in Cottus bairdi. Environmental Biology of Fishes 24:231-236.

GORE, J.A. 1987. Development and applications of macroinvertebrate instream flow models
for regulated flow management. In: J.F. Craig and J.B. Kemper, eds. Regulated
streams: advances in ecology. Plenum Press, New York. pp. 99-115.

GORE, J.A. and R.D. JUDY. 1981. Predictive models of benthic macroinvertebrate density for
use in instream flow studies and regulated flow management. Can. Journ. Fish. Aquat.
Sci. 38:1363-1370.

GORE, J.A., J.B. LAYZER, and J. MEAD. 2001. Macroinvertebrate instream flow studies after
20 years: a role in stream management and restoration. Regulated Rivers: Research and
Management 17:527-542

KARR J. R. 1981. Assessment of biotic integrity using fish communities. Fisheries. 6(6):2127.

KINGSOLVING, A.D., and M.B. BAIN. 1993. Fish assemblage recovery along a riverine
disturbance gradient. Ecological Applications 3:531-544.

115
KREBS, C.J. 1999. Ecological methodology, second edition. Addison Wesley Longman, New
York.

HAND, J., J. COL, and L. LORD. 1996. Northeast Florida District Water Quality Assessment
1996 305 (b) Technical Appendix. Bureau of Surface Water Management, Florida
Department of Environmental Protection.

HAWKINS, C.P. and J.R. SEDELL. 1981. Longitudinal and seasonal changes in functional
organization of macroinvertebrate communities in four Oregon streams. Ecology
62(2):387-397.

HYNES, H.B.N. 1970. The ecology of running waters. University of Toronto Press.

JACOBI, G.Z. 1978. An inexpensive circular sampler for collecting benthic macroinvertebrates
in streams. Archive fr Hydrobiologia 83:126-131.

MARELLA, R.L. 2008. Water use in Florida, 2005 and trends 1950-2005. U.S. Geological
Survey Fact Sheet 2008-3080. Available at:
http://fl.water.usgs.gov/infodata/wateruse.html

MERRITT, R.W., K.W. CUMMINS, M.B. BERG, J.A. NOVAK, M.J. HIGGINS, K.J.
WESSEL, and J.L. LESSARD. 2002. Development and application of a
macroinvertebrate functional- group approach in the bioassessment of remnant river
oxbows in southwest Florida. Journal North American Benthological Society 21(2):290-
310.

MERRITT, R.W., V.H. RESH, K.W. CUMMINS, and D.P. BATZER. 2008. Sampling aquatic
insects. In R.W. Merritt, K.W. Cummins, and M.B. Berg (eds.). An introduction to the
aquatic insects of North America, fourth edition. Kendall/Hunt Publishing Co., Dubuque,
IA. xvi + 39 color plates + 1158 pp.

MCCUNE, B. and M.J. MEFFORD. 1999. PC-ORD. Multivariate analysis of ecological data,
Version 5. MjM Software Design, Gleneden Beach, OR.

MILHOUSE, R.T., D.L. WEGNER, and T. WADDLE. 1984. Users guide to the physical
habitat simulation system. U.S. Fish and Wildlife Service, FWS/OBS 81/43.

MILHOUSE, R.T., M.A. UPDIKE, and D.M. SCHNEIDER. 1989. Physical habitat simulation
system reference manual, version II. U.S. Fish and Wildlife Service Biological Report
89.

MILLER D. L., and thirteen coauthors. 1988. Regional applications of an index of biotic
integrity for use in water resource management. Fisheries. 13(5):1220.

PIELOU, E.C. 1977. Ecological diversity. John Wiley and Sons, New York.

116
POFF, N.L. AND J.V. WARD. 1989. Implications of streamflow variability and predictability
for lotic community structure: a regional analysis of streamflow patterns. Canadian
Journal of Aquatic Sciences 46:1805-1818.

POFF, N.L., J.D. ALLAN, M.B. BAIN, J.R. KARR, K.L. PRESTEGAARD, B.D. RICHTER,
R.E. SPARKS, AND J.C. STROMBERG. 1997. The natural flow regime, a paradigm
for river conservation and restoration. Bioscience 47(11):769-784.

POWER, M.E. 1987. Predator avoidance by grazing fishes in temperate and tropical streams:
importance of stream depth and prey size, p 333-353. In W.C. Kerfoot and A. Sih (Eds.).
Predation: direct and indirect impacts on aquatic communities. University Press of New
England, Hanover, N.H.

POWERS, C.F. and A. ROBERTSON. 1967. Design and evaluation of an all-purpose benthos
sampler, pp. 126-131. In J.C. Ayers and D.C. Chandler (eds). Studies on the environment
and eutrophication of Lake Michigan. University of Michigan Great Lakes Research
Division Special Report 30.

RAO, D.V. and D.A. CLAPP. 1996. Preliminary evaluation of the impacts of spring discharge
reductions on the flows of receiving waters and natural systems, central Florida.
SJRWMD Special Publication SJ96-SP3. Palatka, FL. 71 pp.

RICHTER, B.D., J.V. BAUMGARTNER, J. POWELL, and D.P. BRAUN. 1996. A method for
assessing hydrologic alteration within ecosystems. Conservation Biology 10:1163-1174.

RICHTER, B.D, J.V. BAUMGARTNER, R. WIGINGTON, and D.P. BRAUN. 1997. How
much water does a river need? Freshwater Biology 37:231-249.

RESH, V.H., A.V. BROWN, A.P. COVICH, M.E. GURTZ, H.W. LI, G.W. MINSHALL, S.R.
REICE, A.L. SHELDON, J.B. WALLACE, and R. WISSMAR. 1988. The role of
disturbance in stream ecology. Journal of the North American Benthological Society
7(4)433-455.

SAS INSTITUTE, INC. 2007. SAS Institute Inc., Cary, NC.

SCHAEFER, J.F., W. I. LUTTERSCHMIDT, and L.G. HILL. 1999. Physiological performance

and stream microhabitat use by the centrarchids Lepomis megalotis and Lepomis
macrochirus. Environmental Biology of Fishes 54: 303312, 1999.

SCHLOSSER, I.J. 1985. Flow regime, juvenile abundance, and the assemblage structure of
stream fishes. Ecology 66:1484-1490.

SCHLOSSER, I.J. 1987. The role of predation in age and size related habitat use by stream
fishes. Ecology 66: 651-659.

117
SCOTT, T.M, J.M. LLOYD, and G. MADDOX. 1991. Floridas ground water quality
monitoring program hydrogeological framework. Florida Geological Survey Special
Publication No. 32.

SIMPSON, K.W. and R.W. BODE. 1980. Common larvae of Chironomidae (Diptera) from
New York state streams and rivers. Bulletin No. 439. New York State Museum. 105 pp.

SMITH, D.I. AND P. STOPP. 1978. The river basin: an introduction to the study of hydrology.
Cambridge University Press, London. 120 pp.

SMITH, S.K. 2005. Florida population growth: past, present, and future. University of Florida
Dept. of Economic and Business Research.

STALNAKER, C., B.L. LAMB, J. HENRIKSEN, K. BOVEE, J. BARTHOLOW. 1995. The

instream flow incremental methodology. A primer for IFIM. Biological Report 29,
National Biology Service, U.S. Fish and Wildlife Service.

STATZNER, B, J.A. GORE, and V.H. RESH. 1988. Hydraulic stream ecology: observed
patterns and potential applications. Journal of the North American Benthological Society
7(4)307-360.

SYSTAT SOFTWARE, INC. 2007. Systat 12.0. Richmond, CA.

THIENNEMANN, A. 1925. Die Binnengewasser Mitteleuropas. Eine limnlogische

Einfuhrung. Die Binnengewasser I:1.255.

VERGARA, B.A., ed. 1994. Water supply needs and sources assessment, 1994, St. Johns River
Water Management District. SJRWMD Technical Publication SJ94-7. Palatka, FL.
132pp.

WALLACE, J.B. 1975. Food partitioning in net-spinning Trichoptera larvae: Hydropsyche

venularis, Cheumatopsyche etrona, and Macronema zebratum (Hydropsychidae). Annals
Entomological Society of America 68:463-472.

WARD, J.V., and J.A. STANFORD. 1995. Ecological connectivity in alluvial river ecosystems
and its disruption by flow regulation. Regulated Rivers 11:105-119.

WARREN, G.L., T.J. FERRING, and D.A. HOHLT. 2006. Aquatic invertebrate communities
at five locations in the Alafia River watershed, Hillsborough County, Florida. Final
Report to Southwest Florida Water Management District. Florida Fish and Wildlife
Research Institute Library No. F6306-02-05-F.

WARREN, G.L., D.A. HOHLT, C.E. CICHRA, and D. VAN GENECHTEN. 2000. Fish and
aquatic invertebrate communities of the Wekiva and Little Wekiva Rivers: A baseline
evaluation in the context of Floridas minimum flows and levels statutes. Special
Publication SJ2000-SP4. St. Johns River Water Management District, Palatka, FL.

118
APPENDIX 1. Mean (no m-2), coefficient of variation (cv), and percent composition
(%) of aquatic invertebrate taxa collected from areally dominant habitats in the northern
Withlacoochee River, Florida, study area, 2005 - 2007.

119
APPENDIX 1. Mean (no m-2), coefficient of variation (cv), and percent composition (%) of aquatic invertebrate taxa collected from areally
dominant habitats in the northern Withlacoochee River, Florida, study area, 2005 - 2007.
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Porifera --- --- --- --- P ---

Cnidaria
Hydrozoa
Cordylophora lacustris P P P P P ---

Turbellaria 70 (1.68) 28 (2.49) 1160 (1.09) 3 (3.81) 522 (1.21) 19 (1.73)

0.2 0.2 2.1 <0.1 0.9 0.1

Nemertea
Tetrastemmatidae
Prostoma sp. 99 (1.36) 214 (2.9) 642 (1.11) 27 (2.03) 107 (1.34) 19 (1.73)
0.3 1.6 1.2 0.1 0.2 0.1

Nematoda 140 (1.22) 284 (1.71) 317 (1.03) 512 (2.24) 372 (1.26) 261 (1.00)
0.4 2.1 0.6 1.7 0.6 1.5

Annelida
Aphanoneura
Aeolosomatidae
Aeolosoma sp. --- 2 (4.36) 5 (4.24) --- 17 (2.64) ---
<0.1 <0.1 <0.1

Oligochaeta (total) 5,003 (1.09) 2,802 (1.33) 4,854 (1.23) 582 (1.53) 3,317 (1.45) 319 (0.59)
12.9 20.5 8.8 2.0 5.5 1.8

120
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Enchytraeidae (total) --- 3 (3.51) 27 (1.99) 6 (2.95) 59 (1.90) 5 (1.73)

<0.1 <0.1 <0.1 0.1 <0.1

Naididae (total) 369 (2.07) 753 (3.87) 101 (1.82) 394 (2.16) 791 (0.91) 251 (0.79)
0.9 5.5 0.2 1.3 1.3 1.4

Amphichaeta leydigii --- --- --- 2 (5.48) --- ---

<0.1

Bratislavia unidentata 5 (3.61) --- --- --- --- ---

<0.1

Chaetogaster sp. --- 2 (6.25) 3 (3.44) 1 (3.18) 52 (1.94) 5 (1.73)

<0.1 <0.1 <0.1 0.1 <0.1

Chaetogaster diaphanus --- --- --- --- 12 (3.87) ---

<0.1

Dero sp. --- 6 (5.13) --- --- --- ---

<0.1

Dero digitata complex --- 215 (3.79) --- --- --- ---
1.6

Dero pectinata 7 (3.61) 6 (5.13) --- --- --- ---

<0.1 <0.1

121
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Dero trifida 5 (3.61) 65 (4.54) --- --- --- ---

<0.1 0.5

Nais sp. 4 (2.44) --- 3 (3.28) 15 (2.22) 45 (1.78) 5 (1.73)

<0.1 <0.1 0.1 0.1 <0.1

Nais behningi 48 (2.19) 10 (4.93) 16 (1.80) 188 (2.16) 184 (1.11) 24 (1.26)
0.1 0.1 <0.1 0.6 0.3 0.1

Nais communis complex 89 (1.93) 2 (4.36) 40 (2.26) 139 (2.60) 296 (1.69) 212 (0.85)
0.2 <0.1 0.1 0.5 0.5 1.2

Nais simplex --- 1 (6.25) --- 4 (3.60) 26 (3.07) ---

<0.1 <0.1 <0.1

Nais variabilis 84 (3.56) 108 (5.54) 3 (3.28) 1 (3.89) 42 (3.87) ---

0.2 0.8 <0.1 <0.1 0.1

Pristina aequiseta 47 (3.61) 215 (4.15) 4 (3.10) 4 (5.00) 5 (3.87) ---

0.1 1.6 <0.1 <0.1 <0.1

Pristina leidyi 34 (3.61) 62 (4.75) --- --- 8 (3.87) ---

0.1 0.5 <0.1

Pristina probiscidea --- 5 (6.25) --- --- --- ---

<0.1

122
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Pristina synclites --- 41 (4.61) --- --- --- ---

0.3

Pristinella sp. --- 3 (6.25) 5 (3.71) 1 (5.48) --- ---

<0.1 <0.1 <0.1

Pristinella jenkinae --- --- --- --- 7 (3.87) ---

<.01

Pristinella osborni 39 (2.13) 6 (3.20) 23 (2.23) 35 (4.24) 28 (1.79) ---

0.1 <0.1 <0.1 0.1 <0.1

Pristinella sima 4 (3.61) --- --- --- --- ---

<0.1

Slavina appendiculata <1 (3.61) --- --- <1 (5.48) 44 (1.44) ---
<0.1 <0.1 0.1

Stephensoniana trivandrana --- --- 1 (4.24) --- --- ---

<0.1

Stylaria lacustris --- --- --- --- 6 (3.87) ---

<0.1

unidentifiable immature Naididae 3 (2.58) 3 (3.51) 3 (2.40) 5 (3.25) 36 (1.58) ---

<0.1 <0.1 <0.1 <0.1 0.1

123
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Tubificidae (total) --- 81 (2.45) 40 (2.00) 1 (3.81) 118 (2.09) 5 (1.73)

0.6 0.1 <0.1 0.2 <0.1

Aulodrilus pigueti --- --- --- <1 (5.48) --- ---

<0.1

Limnodrilus hoffmeisteri --- 14 (6.25) --- --- 7 (2.77) ---

0.1 <0.1

Haber speciosus --- --- 14 (3.27) --- --- ---

<0.1

UIWCS --- 27 (3.33) 19 (2.84) --- 23 (3.12) 5 (1.73)

0.2 <0.1 <0.1 <0.1

UIWOCS --- 44 (2.04) 8 (1.92) 1 (5.48) 87 (1.87) ---

0.3 <0.1 <0.1 0.1

Lumbriculidae (total) --- 1 (6.25) 3 (1.93) --- --- 9 (0.87)

<0.1 <0.1 <0.1

Eclipidrilus sp. --- 1 (6.25) 2 (2.30) --- --- ---

<0.1 <0.1

Eclipidrilus paulustris --- --- 1 (4.24) --- --- 9 (0.87)

<0.1 <0.1

124
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

unidentifiable immature Oligochaeta 4 (3.61) --- 5 (3.20) <1 (5.48) --- ---
<0.1 <0.1 <0.1
Hirudinia
Glossiphoniidae <1 (3.61) --- 5 (3.76) --- --- 10 (1.73)
<0.1 <0.1 0.1

Mollusca
Gastropoda (total) 107 (1.62) 246 (2.08) 835 (1.36) 92 (1.75) 1,679 (1.09) 48 (0.35)
0.3 1.8 1.5 0.3 2.8 0.3

Ancylidae (imm.) --- 7 (4.61) 29 (1.19) 10 (3.73) 475 (2.09) 19 (0.87)

0.1 0.1 <0.1 0.8 0.1

Ferrissia sp. --- --- --- <1 (5.48) --- ---

<0.1

Hydrobiidae (imm.) 11 (2.60) 12 (2.70) 10 (2.03) 16 (4.19) 5 (3.87) ---

<0.1 0.1 <0.1 0.1 <0.1

Planorbidae (imm.) 9 (1.92) 11 (2.79) 121 (1.24) 14 (2.25) 40 (1.97) 19 (1.73)

<0.1 0.1 0.2 <0.1 0.1 0.1

Gyraulus parvus --- --- 1 (2.91) --- --- ---

<0.1

Micromenetus sp. <1 (3.61) --- --- <1 (5.48) 56 (3.87) ---
<0.1 <0.1 0.1
125
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Micromenetus dilatatus --- --- 1 (4.24) --- 432 (1.75) 10 (1.73)

<0.1 0.7 0.1

Micromenetus floridensis --- --- 3 (4.24) --- --- ---

<0.1

Lymnaeidae
Pseudosuccinea columella 2 (3.61) --- 17 (3.90) --- --- ---
<0.1 <0.1

Physidae 2 (2.44) 8 (4.02) 255 (2.95) 12 (3.57) --- ---

<0.1 0.1 0.5 <0.1

Physella sp. --- --- --- 2 (4.90) --- ---

<0.1

Physella cubensis --- --- 10 (2.47) --- --- ---

<0.1

Pleuroceridae
Elimia floridensis 35 (1.74) 179 (2.33) 114 (1.73) 5 (3.08) 558 (2.51) ---
0.1 1.3 0.2 <0.1 0.9

Viviparidae
Viviparus georgianus --- --- 1 (4.24) 1 (5.48) 13 (2.74) ---
<0.1 <0.1 <0.1

126
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

unidentifiable imm. Gastropoda 48 (2.43) 25 (1.99) 260 (1.38) 36 (2.00) 100 (2.20) ---
0.1 0.2 0.5 0.1 0.2

Pelecypoda 131 (1.55) 2,045 (1.81) 665 (1.14) 45 (2.00) 342 (2.57) 155 (0.78)
0.3 15 1.2 0.2 0.6 0.9

Corbiculidae
Corbicula fluminea 22 (1.76) 779 (2.46) 103 (0.88) 9 (2.69) 342 (2.57) 155 (0.78)
0.1 5.7 0.2 <0.1 0.6 0.9

Sphaeriidae (imm.) --- 2 (6.25) --- --- --- ---

<0.1

Unionidae (imm.) --- 1 (6.25) --- --- --- ---

<0.1

immature Pelecypoda 110 (1.80) 1,263 (2.72) 562 (1.28) 33 (2.74) --- ---
0.3 9.2 1.0 0.1
Arthropoda
Crustacea
Amphipoda (total) 71 (1.60) 42 (2.01) 174 (1.78) 966 (2.61) 5,627 (1.17) 97 (0.69)
0.2 0.3 0.3 3.3 9.4 0.5

Gammaridae
Gammarus sp. <1 (3.61) 1 (6.25) 40 (2.70) 2 (3.06) 2,905 (2.06) 68 (0.65)
<0.1 <0.1 0.1 <0.1 4.9 0.4

127
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Hyalellidae
Hyalella azteca 33 (1.74) 34 (2.22) 103 (1.55) 903 (2.64) 2,196 (1.80) ---
0.1 0.2 0.2 3.1 3.7

immature Amphipoda 37 (2.13) 7 (3.52) 31 (2.23) 62 (2.60) 525 (1.42) 29 (1.00)

0.1 <0.1 0.1 0.2 0.9 0.2

Isopoda (total) --- 6 (6.25) 2 (3.07) 6 (2.18) 250 (2.38) 19 (1.73)

<0.1 <0.1 <0.1 0.4 0.1

Caecidotea sp. --- 6 (6.25) --- 4 (2.76) 250 (2.38) 19 (1.73)

<0.1 <0.1 0.4 0.1

unidentifiable imm. Isopoda --- --- 2 (3.07) 1 (5.48) --- ---

<0.1 <0.1

Decapoda
Cambaridae
Procambarus sp. imm. --- --- 2 (3.07) 1 (5.48) --- ---
<0.1 <0.1

Palaemonetes paludosus --- --- --- --- 6 (3.87) ---

<0.1

128
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Aquatic Acari (total) 1,075 (0.82) 330 (1.58) 5,560 (1.05) 2,367 (0.90) 1,711 (1.15) 319 (0.74)
2.8 2.4 10.1 8.0 2.9 1.8

Hydracarina 659 (0.71) 268 (1.66) 4,168 (1.17) 1,693 (0.74) 1,341 (1.05) 280 (0.96)
1.7 2.0 7.5 5.8 2.2 1.6

Oribatidae 416 (1.78) 61 (1.87) 1,432 (2.08) 519 (1.82) 369 (1.67) 39 (0.87)
1.1 0.4 2.6 1.8 0.6 0.2

Insecta
Collembola --- 3 (4.61) 1 (4.24) <1 (5.48) 8 (3.87) ---
<0.1 <0.1 <0.1 <0.1

Sminthuridae --- --- 1 (4.24) <1 (5.48) --- ---

<0.1 <0.1

Collembola unidentifiable e.i. --- 1 (6.25) --- --- 8 (3.87) ---

<0.1 <0.1

Ephemeroptera (total) 788 (1.19) 48 (2.46) 3,104 (1.03) 1,215 (0.80) 2,596 (1.01) 261 (0.62)
2.0 0.3 5.6 4.1 4.3 1.5
Eurylophella doris --- --- --- --- 8 (3.87) ---
<0.1

Heptageniidae (e.i. or dam.) 2 (3.61) 1 (6.25) 43 (1.34) 254 (1.26) 35 (2.66) 39 (0.87)
<0.1 <0.1 0.1 0.9 0.1 0.2
129
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Stenacron sp. --- --- --- 1 (5.48) --- 10 (1.73)

<0.1 0.1

Stenacron interpunctatum --- --- 1 (4.24) --- --- ---

<0.1

Maccaffertium sp. --- --- 47 (1.52) 106 (1.34) 7 (3.87) ---

0.1 0.4 <0.1

Maccaffertium exiguum --- --- 5 (1.75) 36 (3.13) 7 (3.87) ---

<0.1 0.1 <0.1
Isonychiidae
Isonychia sp. --- --- --- 1 (5.48) --- ---
<0.1

Leptohyphidae (e.i.) 59 (2.01) --- 300 (1.80) 136 (2.47) 393 (1.67) 97 (1.73)
0.2 0.5 0.5 0.7 0.5
Asioplax dolani --- --- --- 1 (5.48) --- ---
<0.1

Tricorythodes albilineatus 227 (1.62) 8 (4.76) 1,772 (1.32) 280 (1.66) 723 (2.03) ---
0.6 0.1 3.2 1.0 1.2

Odonata
Anisoptera (total) 3 (2.58) 8 (2.52) 5 (2.67) 8 (2.13) 66 (1.84) 19 (1.73)
<0.1 0.1 <0.1 <0.1 0.1 0.1
130
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Corduliidae
Neurocordulia sp. --- --- --- 4 (3.02) --- ---
<0.1

Gomphidae e.i. --- 6 (2.64) 1 (4.24) --- 19 (2.64) ---

<0.1 <0.1 <0.1

Dromogomphus spinosus --- 1 (6.25) --- --- --- ---

<0.1

Hagenius brevistylus --- --- 1 (4.24) --- --- ---

<0.1

Progomphus obscurus --- --- --- --- --- 19 (1.73)

0.1

Libellulidae e.i. 2 (3.61) --- 3 (4.24) 4 (2.56) 20 (3.16) ---

<0.1 <0.1 <0.1 <0.1

Macromiidae
Macromia sp. --- --- --- --- 27 (2.35) ---
<0.1

imm. Anisoptera <1 (3.61) 1 (6.25) --- --- --- ---

<0.1 <0.1

131
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Zygoptera (total) --- 1 (6.25) 24 (2.26) 5 (2.34) 126 (1.22) ---

<0.1 <0.1 <0.1 0.2
Calopterygidae
Hetaerina titia --- --- --- --- 12 (2.90) ---
<0.1

Coenagrionidae e.i. --- --- 5 (4.24) 1 (4.44) 23 (2.23) ---

<0.1 <0.1 <0.1

Argia sp. --- --- 10 (3.29) 3 (3.97) 10 (3.87) ---

<0.1 <0.1 <0.1

Argia moesta --- --- 1 (4.24) <1 (5.48) 3 (3.87) ---

<0.1 <0.1 <0.1

Enallagma sp. --- --- --- --- 23 (3.54) ---

<0.1

Enallagma coecum --- --- --- --- 3 (3.87) ---

<0.1

Enallagma signatum --- --- --- --- 4 (2.97) ---

<0.1

imm. Zygoptera --- 1 (6.25) 7 (1.83) 1 (3.31) 49 (1.94) ---

<0.1 <0.1 <0.1 0.2
132
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Plecoptera (total) --- 3 (4.61) 23 (1.81) 14 (1.84) --- 10 (1.73)

<0.1 <0.1 <0.1 0.1

Perlidae e.i. --- --- 2 (4.24) 2 (2.63) --- ---

<0.1 <.01

Agnetina annulipes --- --- 1 (4.24) --- --- ---

<0.1

Neoperla sp. --- 1 (6.25) 6 (1.97) 1 (3.81) --- ---

<0.1 <0.1 <0.1

Neoperla clymene --- --- 8 (2.13) 5 (3.22) --- 10 (1.73)

<0.1 <0.1 0.1

unidentifiable imm. Plecoptera --- 2 (6.25) 7 (2.41) 5 (2.75) --- ---

<0.1 <0.1 <0.1

Hemiptera 4 (1.99) 1 (6.25) 1 (4.24) --- 14 (2.96) ---

<0.1 <0.1 <0.1 <0.1

Mesoveliidae
Mesovelia amoena 3 (2.58) --- --- --- --- ---
<0.1

unidentifiable imm. Hemiptera --- --- 1 (4.24) --- 14 (2.96) ---

<0.1 <0.1
133
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Megaloptera 5 (2.90) --- 105 (2.40) 9 (2.04) --- 5 (1.73)

<0.1 0.2 <0.1 <0.1

Corydalus cornutus 5 (2.90) --- 105 (2.40) 9 (2.04) --- 5 (1.73)

<0.1 0.2 <0.1 <0.1

Trichoptera (total) 2,000 (0.94) 165 (3.18) 2,916 (0.95) 3,691 (1.16) 7,542 (0.77) 431 (0.37)
5.1 1.2 5.3 12.5 12.6 2.4

Hydropsychidae (total) 323 (1.31) 14 (4.30) 461 (1.84) 968 (1.30) 63 (3.52) 29 (1.31)
0.8 0.1 0.8 3.3 0.1 0.2

Hydropsychidae e.i. 67 (1.66) --- 254 (2.19) 308 (1.55) 17 (3.87) 10 (1.73)
0.2 0.5 1.0 <0.1 0.1

Cheumatopsyche sp. 43 (1.39) 3 (4.61) 72 (2.27) 204 (1.14) 39 (3.33) 15 (0.99)

0.1 <0.1 0.1 0.7 0.1 0.1

Hydropsyche sp. 103 (1.97) 1 (6.25) 62 (1.70) 182 (1.71) 7 (3.87) 5 (1.73)
0.3 <0.1 0.1 0.6 <0.1 <0.1
Hydropsyche rossi 110 (1.41) 9 (5.51) 75 (1.67) 346 (1.26) --- ---

0.3 0.1 0.1 1.2

Hydroptilidae (total) 1386 (1.02) 74 (3.21) 2,061 (1.01) 1,291 (0.90) 4,422 (0.91) 193 (0.96)
3.6 0.5 3.7 4.4 7.4 1.1

134
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Hydroptilidae e.i. 202 (0.99) 64 (3.75) 169 (0.99) 222 (1.25) 1,505 (1.39) 39 (1.73)
0.5 0.5 0.3 0.8 2.5 0.2

Hydroptila sp. 1192 (1.06) 49 (3.42) 1,566 (1.12) 1,000 (1.15) 1,748 (0.83) 39 (0.87)
3.1 0.4 2.8 3.4 2.9 0.2

Mayatrichia ayama 2 (3.61) --- 5 (2.58) 36 (2.97) 3 (3.87) ---

<0.1 <0.1 0.1 <0.1

Mayatrichia sp. or Neotrichia

sp. 2 (3.61) --- --- --- --- ---
<0.1

Neotrichia sp. <1 (3.61) 16 (5.81) 127 (3.17) 56 (2.57) 1,078 (1.10) 116 (1.00)
<0.1 0.1 0.2 0.2 1.8 0.7

Orthotrichia sp. --- --- --- <1 (5.48) 4 (3.87) ---

<0.1 <0.1

Oxyethira sp. 18 (1.87) 2 (6.25) 193 (1.87) 89 (2.19) 73 (2.17) ---

<0.1 <0.1 0.3 0.3 0.1

Leptoceridae (total) 27 (1.55) 72 (5.16) 108 (1.86) 215 (4.15) 1,687 (1.45) 140 (0.69)
0.1 0.5 0.2 0.7 2.8 0.8

Leptoceridae e.i. --- 2 (4.36) 11 (1.84) 6 (2.57) 14 (3.61) ---

<0.1 <0.1 <0.1 <0.1
135
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Ceraclea sp. --- --- 1 (2.91) 9 (4.24) 5 (3.87) 5 (1.73)

<0.1 <0.1 <0.1 <0.1

Ceraclea flava --- --- 1 (4.24) --- --- ---

<0.1

Nectopsyche sp. <1 (3.61) 2 (6.25) 7 (1.78) 3 (3.40) 759 (1.51) 48 (1.25)
<0.1 <0.1 <0.1 <0.1 1.3 0.3

Nectopsyche exquisita --- 3 (4.61) 2 (4.24) 2 (2.86) 469 (1.89) 39 (1.73)

<0.1 <0.1 <0.1 0.8 0.2

Nectopsyche pavida <1 (3.61) --- 1 (4.24) --- --- ---

<0.1 <0.1

Oecetis sp. 17 (1.74) 3 (4.61) 29 (1.84) 28 (1.33) 427 (1.20) 10 (1.73)

<0.1 <0.1 0.1 0.1 0.7 0.1

Oecetis avara 7 (2.06) 2 (6.25) 55 (2.68) 2 (3.38) 10 (3.87) 39 (1.15)

<0.1 <0.1 0.1 <0.1 <0.1 0.2

Oecetis persimilis --- --- --- 2 (4.43) --- ---

<0.1

Oecetis sp. A --- 1 (6.25) --- --- --- ---

<0.1
136
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Triaenodes sp. --- --- --- --- 5 (3.87) ---

<0.1

Philopotamidae e.i. --- --- 5 (2.48) 8 (4.81) --- ---

<0.1 <0.1

Chimarra sp. 8 (2.15) --- 36 (1.14) 106 (3.82) --- ---

<0.1 0.1 0.4

Polycentropodidae e.i. 2 (2.44) --- 10 (2.81) 56 (1.86) 8 (3.87) ---

<0.1 <0.1 0.2 <0.1

Cernotina sp. --- --- 6 (2.93) 16 (2.11) --- ---

<0.1 0.1

Cyrnellus fraternus --- --- --- 14 (1.41) --- ---

<0.1

Neureclipsis sp. --- --- --- <1 (5.48) 5 (3.87) ---

<0.1 <0.1

unidentifiable imm. Trichoptera 235 (1.47) 6 (3.20) 226 (1.42) 983 (2.44) 1,367 (1.30) 68 (1.73)
0.6 <0.1 0.4 3.3 2.3 0.4

Neuroptera (total) --- --- --- --- 4 (3.87) ---

<0.1
137
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Sisyridae
Sisyra sp. --- --- --- --- 4 (3.87) ---
<0.1

Lepidoptera (total - e.i.) --- --- 1 (4.24) --- --- ---

<0.1

Coleoptera (total) 4,914 (1.67) 239 (1.17) 5,756 (1.24) 3,049 (0.80) 3,025 (1.05) 406 (0.12)
12.6 1.8 10.4 10.4 5.1 2.3

Curculionidae --- --- 1 (4.24) 2 (5.48) --- ---

<0.1 <0.1

Dytiscidae e.i. --- --- 1 (4.24) --- 5 (3.87) ---

<0.1 <0.1

Elmidae (total) 4,909 (1.67) 236 (1.19) 9,163 (2.03) 3,012 (0.80) 3,011 (1.05) 396 (0.11)
12.6 1.7 16.6 10.2 5.0 2.2

Elimidae (e.i.) 185 (1.95) 16 (3.59) 204 (1.52) 68 (2.06) --- 10 (1.73)
0.5 0.1 0.4 0.2 0.1
Dubiraphia vittata 14 (2.49) 58 (2.00) 54 (1.49) 8 (2.69) 594 (0.92) 32 (0.91)
<0.1 0.4 0.1 <0.1 1.0 0.2

Microcylloepus pusillus 23 (1.65) 1 (6.25) 105 (1.91) 84 (1.33) 56 (2.47) ---

0.1 <0.1 0.2 0.3 0.1
138
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Stenelmis sp. 4,536 (1.77) 162 (1.36) 8,783 (2.08) 2,904 (0.76) 2,299 (1.24) 358 (0.17)
11.8 1.2 15.9 9.9 3.8 2.0

Stenelmis antennalis --- --- 1 (4.24) --- --- ---

<0.1

Stenelmis convexula 12 (2.38) --- 11 (1.84) 5 (3.81) --- ---

<0.1 <0.1 <0.1

Stenelmis hungerfordi 133 (2.05) --- 64 (1.29) 62 (1.21) 45 (2.46) ---

0.3 0.1 0.2 0.1

Stenelmis hungerfordi or
lignicola 6 (3.08) --- 1 (2.91) 1 (5.48) --- ---
<0.1 <0.1 <0.1

Gyrinidae e.i. 1 (3.61) --- --- --- --- ---

<0.1

Dineutus sp. 2 (3.61) --- --- 4 (5.48) --- ---

<0.1 <0.1

Haliplidae
Peltodytes sp. --- 2 (6.25) 5 (2.91) 1 (5.48) --- ---
<0.1 <0.1 <0.1
139
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Hydrophilidae (e.i.) 1 (3.61) --- 79 (3.89) --- --- 10 (1.73)

<0.1 0.1 0.1

Enochrus sp. 1 (3.61) --- 9 (1.80) 1 (5.48) 8 (3.87) ---

<0.1 <0.1 <0.1 <0.1

Diptera (e.i.) 2 (3.61) --- --- <1 (5.48) --- ---

<0.1 <0.1

Ceratopogonidae (total) 1,533 (1.58) 683 (1.86) 402 (0.83) 632 (1.04) 476 (1.07) 48 (0.35)
3.9 5.0 0.7 2.1 0.8 0.3

Atrichopogon sp. --- --- 5 (4.24) 21 (5.13) 8 (3.87) ---

<0.1 <0.1 <0.1

Bezzia or Palpomyia 917 (1.51) 230 (2.99) 340 (0.89) 508 (1.13) 375 (1.06) 48 (0.35)
2.4 1.7 0.6 1.7 0.6 0.3

Dasyhelea sp. 43 (3.61) 30 (2.45) 18 (3.76) 1 (3.11) 4 (3.87) ---

0.1 0.2 <0.1 <0.1 <0.1

Probezzia sp. --- 293 (2.27) 8 (2.77) <1 (5.48) 19 (2.64) ---
2.1 <0.1 <0.1 <0.1

Ceratopogonidae e.i. 565 (2.08) 132 (1.90) 5 (3.30) 94 (2.27) 69 (2.72) ---
1.5 1.0 <0.1 0.3 0.1
140
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Chaoboridae (total) --- 1 (6.25) --- --- --- ---

<0.1

Chaoborus punctipennis --- 1 (6.25) --- --- --- ---

<0.1

Chironomidae (total) 22,137 (0.95) 6,447 (1.04) 28,049 (1.05) 15,361 (0.73) 31,720 (0.57) 15,167 (0.58)
57.0 47.2 50.8 52.2 53.0 85.8

Tanypodinae (e.i.) --- 16 (2.68) 45 (3.09) 30 (4.72) 31 (2.66) ---

0.1 0.1 0.1 0.1

Meropelopia sp. --- --- --- 8 (5.48) --- ---

<0.1

Pentaneurini e.i. 15 (3.61) 33 (3.02) 57 (1.95) 11 (4.27) 51 (2.740 ---

<0.1 0.2 0.1 <0.1 0.1

Ablabesmyia sp. --- 9 (4.36) --- 9 (3.90) 303 (1.84) ---

0.1 <0.1 0.5

Ablabesmyia mallochi 2 (3.61) 17 (4.83) 3 (4.24) 3 (5.48) 463 (2.50) ---

<0.1 0.1 <0.1 <0.1 0.8

Hayesomyia senata --- --- 15 (4.24) --- --- ---

<0.1

141
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Labrundinia maculata --- --- --- 4 (5.48) --- ---

<0.1

Labrundinia pilosella --- --- --- --- 17 (3.87) ---

<0.1

Larsia sp. 3 (3.61) 6 (6.25) 16 (4.24) --- --- ---

<0.1 <0.1 <0.1

Larsia decolorata 16 (3.39) --- 18 (3.65) 35 (2.74) 32 (3.87) ---

<0.1 <0.1 0.1 0.1

Monopelopia boliekae --- --- --- 3 (5.48) --- ---

<0.1

Nilotanypus fimbriatus 23 (3.45) 2 (6.25) --- 41 (2.03) 54 (2.83) ---

0.1 <0.1 0.1 0.1

Pentaneura sp. --- --- --- 7 (5.48) 14 (3.87) ---

<0.1 <0.2

Pentaneura inconspicua 23 (3.61) --- 8 (4.24) 19 (2.77) 1,024 (1.40) 19 (1.73)

0.1 <0.1 0.1 1.7 0.1

Rheopelopia sp. --- 5 (6.25) --- 4 (5.48) --- ---

<0.1 <0.1
142
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Thienemannimyia complex 23 (3.61) 123 (2.81) 82 (3.84) 60 (2.41) --- ---

0.1 0.9 0.1 0.2

Procladius sp. --- 14 (6.25) --- --- --- ---

0.1

Djalmabatista pulchra --- 2 (6.25) --- --- --- ---

<0.1

Tanypus sp. --- 58 (3.24) --- --- --- ---

0.4

Tanypus carinatus --- 25 (4.52) --- --- --- ---

0.2

Tanypus punctipennis --- 10 (3.91) --- --- --- ---

0.1

Diamesinae
Potthastia longimana --- --- --- 13 (3.12) --- ---
<0.1

Orthocladiinae e.i. 242 (1.10) 51 (2.23) 432 (1.61) 89 (2.46) 509 (1.48) 155 (0.57)
0.6 0.4 0.8 0.3 0.9 0.9

Corynoneura sp. --- 31 (1.82) --- 21 (2.05) 289 (1.35) 928 (0.87)
0.2 0.1 0.5 5.2
143
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Corynoneura lobata --- 5 (6.25) --- --- 35 (3.87) ---

<0.1 0.1

Corynoneura species B 99 (2.51) 26 (2.09) 43 (2.31) 50 (3.06) 592 (1.29) 8,884 (0.87)
0.3 0.2 0.1 0.2 1.0 50.2

Corynoneura lobata or sp. B --- 8 (6.25) --- --- --- ---

0.1

Corynoneura species G --- --- --- --- 39 (3.87) ---

0.1

Corynoneura lobata or sp. G --- --- 15 (2.91) --- 59 (3.87) 309 (1.10)
<0.1 0.1 1.7

Cricotopus sp. 353 (2.56) 18 (4.74) 172 (2.23) 106 (1.85) 1,119 (2.55) 19 (1.73)
0.9 0.1 0.3 0.4 1.9 0.1

Cricotopus albiforceps 23 (3.61) 3.41 (6.25) 34 (4.24) 60 (2.73 ) 195 (1.59) 97 (0.92)
0.1 <0.1 0.1 0.2 0.3 0.5

Cricotopus bicinctus 688 (2.43) 33 (4.73) 853 (1.80) 228 (1.92) 4,673 (1.05) 174 (1.45)
1.8 0.2 1.5 0.8 7.8 1.0

144
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Cricotopus trifasciatus --- --- --- 1 (5.48) --- ---

<0.1

Cricotopus albiforceps or
vierriensis 77 (2.28) --- 40 (3.00) 48 (2.90) 40 (2.69) ---
0.2 0.1 0.2 0.1

Cricotopus or Orthocladius 177 (3.20) 24 (4.24) 28 (3.22) 54 (2.64) 643 (3.82) ---
0.5 0.2 0.1 0.2 1.1

Gymnometriocnemus sp. --- --- --- --- 30 (3.87) ---

<0.1

Lopescladius sp. --- 48 (4.34) --- --- --- ---

0.3

Nanocladius sp. 6 (3.61) 5 (4.90) 76 (3.81) 70 (2.97) 567 (1.24) ---

<0.1 <0.1 0.1 0.2 0.9

Nanocladius crassicornus --- --- --- --- 35 (2.67) ---

0.1

Nanocladius crassicornus or
spiniplenus --- --- --- 7 (5.48) --- ---
<0.1

145
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Parakiefferiella sp. 1,680 (1.06) 91 (2.08) 1,015 (0.88) 1,019 (0.84) 1,505 (0.73) 251 (0.74)
4.3 0.7 1.8 3.5 2.5 1.4

Parakiefferiella species B --- 2 (6.25) --- --- --- ---

<0.1

Parakiefferiella species E 577 (1.80) 46 (3.42) 116 (2.08) 259 (2.19) 1,300 (1.39) 271 (0.89)
1.5 0.3 0.2 0.9 2.2 1.5

Parakiefferiella species F 2 (3.61) 115 (3.42) --- 3 (5.48) 65 (3.87) ---

<0.1 0.8 <0.1 0.1

Rheocricotopus robacki --- --- --- --- 20 (3.87) ---

<0.1

Rheosmittia sp. --- 3 (4.61) --- --- --- ---

<0.1

Rheosmittia arcuata --- 1 (6.25) --- --- --- ---

<0.1

Synorthocladius sp. --- --- --- 4 (5.48) 15 (3.87) ---

<0.1 <0.1

Thienemanniella sp. 315 (1.22) 7 (4.61) 116 (1.39) 68 (2.37) 361 (1.46) 145 (0.92)
0.8 0.1 0.2 0.2 0.6 0.8
146
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Thienemanniella lobapodema 198 (2.21) --- 34 (3.54) 186 (1.76) 535 (1.35) ---
0.5 0.1 0.6 0.9

Thienemanniella similis 50 (2.49) 2 (6.25) 56 (2.20) 5 (5.48) 8 (3.87) ---

0.1 <0.1 0.1 <0.1 <0.1

Thienemanniella xena --- --- --- 3 (5.48) 8 (3.87) ---

<0.1 <0.1

Thienemanniella species B 1,023 (1.40) 17 (4.83) 633 (1.69) 446 (1.64) 366 (1.47) 77 (1.15)
2.6 0.1 1.1 1.5 0.6 0.4

Tvetenia sp. --- 2 (6.25) --- 16 (2.73) --- 58 (1.73)

<0.1 0.1 0.3

Tvetenia vitracies 1,229 (2.02) 111 (3.99) 393 (2.07) 966 (1.71) 67 (2.45) ---
3.2 0.8 0.7 3.3 0.1

Chironominae e.i. 146 (2.04) 75 (2.28) 51 (3.14) 25 (2.64) 132 (2.04) ---
0.4 0.6 0.1 0.1 0.2

Chironomini e.i. 23 (3.45) 52 (2.45) --- 42 (2.11) 70 (2.34) ---

0.1 0.4 0.1 0.1

Chernovskiia sp. --- 8 (4.02) --- --- --- ---

0.1
147
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Chironomus sp. 2 (3.61) 35 (4.68) 2.6 (4.24) --- --- ---

<0.1 0.3 <0.1

Cladopelma sp. --- 22 (4.41) --- --- --- ---

0.2

Cryptochironomus sp. 44 (3.61) 106 (2.05) --- --- --- ---

0.1 0.8

Cryptotendipes sp. 3 (3.61) 139 (2.97) 196 (3.14) 15 (2.65) 73 (2.66) ---
<0.1 1 0.4 0.1 0.1

Dicrotendipes sp. 188 (1.87) 21 (3.26) --- 69 (3.24) 34 (2.69) ---

0.5 0.2 0.2 0.1

Dicrotendipes lucifer --- --- --- 7 (5.48) --- ---

<0.1

Dicrotendipes modestus 2 (3.61) 5 (6.25) --- --- --- ---

<0.1 <0.1

Dicrotendipes neomodestus 1,123 (2.05) 79 (4.01) 149 (1.82) 214 (1.68) 519 (1.59) 39 (1.73)
2.9 0.6 0.3 0.7 0.9 0.2

Harnischia complex --- 7 (3.82) --- --- --- ---

<0.1
148
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Nilothauma sp. --- --- --- 4 (5.48) 30 (3.87) ---

<0.1 <0.1

Paracladopelma sp. --- 14 (2.90) --- --- --- ---

0.1

Paracladopelma doris --- 1 (6.25) --- --- --- ---

<0.1

Paracladopelma loganae --- 9 (4.36) --- --- --- ---

0.1

Paracladopelma nereis --- 1 (6.25) --- --- --- ---

<0.1

Paralauterborniella
nigrohalteralis --- 11 (5.05) --- --- --- ---
0.1

Phaenopsectra sp --- --- --- --- --- 58 (1.73)

0.2

Polypedilum sp. 1,390 (2.15) 764 (2.11) 2,707 (1.89) 351 (1.45) 232 (3.27) 1,305 (0.73)
3.6 5.6 4.9 1.2 0.4 7.4

Polypedilum beckae --- --- --- 7 (5.48) --- ---

<0.1
149
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Polypedilum fallax group --- --- --- 16 (4.27) --- ---

0.1

Polypedilum flavum 140 (2.27) 24 (3.011) 62 (2.09) 79 (1.70) 247 (1.49) ---
0.4 0.2 0.1 0.3 0.4

Polypedilum halterale --- 532 (4.12) --- 14 (5.48) 53 (2.96) ---

3.9 <0.1 0.1

Polypedilum illinoense --- 5 (3.75) 7 (4.24) 4 (5.48) 94 (2.03) 213 (0.69)

<0.1 <0.1 <0.1 0.2 1.2

Polypedilum scalaenum group 2 (3.61) 464 (2.98) 57 (2.98) 70 (2.35) 454 (2.65) 454 (0.46)
<0.1 3.4 0.1 0.2 0.8 2.6

Polypedilum tritum --- 1 (6.25) --- --- 6 (3.87) ---

<0.1 <0.1

Robackia sp. --- 34 (3.52) --- --- --- ---

0.3

Robackia claviger --- 115 (2.80) --- --- --- ---

0.8

Stelechomyia perpulchra --- --- --- 724 (1.15) --- 58 (1.73)

2.5 0.3
150
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Stenochironomus sp. 1 (3.61) 16 (4.40) --- 1,046 (1.17) 20 (3.87) 116 (1.73)
<0.1 0.1 3.6 <0.1 0.7

Stictochironomus sp. --- 418 (2.94) --- --- --- ---

3.1

Tribelos fusicorne --- --- --- 25 (3.30) --- ---

0.1

Xenochironomus xenolabis --- --- 18 (3.65) 10 (3.16) --- ---

<0.1 <0.1

Xestochironomus sp. --- 10 (5.58) --- 1,520 (1.24) 20 (3.87) 435 (0.98)
0.1 5.2 <0.1 2.5

Pseudochironomini
Pseudochironomus sp. 7,537 (1.37) 476 (2.26) 4,965 (1.49) 3,068 (1.92) 296 (1.92) 135 (0.89)
19.4 3.5 9.0 10.4 0.5 0.8

Tanytarsini (e.i.) 59 (2.37) 19 (2.57) 41 (3.07) 25 (2.88) 215 (1.52) ---

0.2 0.1 0.1 0.1 0.4

Cladotanytarsus sp. --- 10 (4.39) --- 4 (5.48) 30 (3.87) ---

0.1 <0.1 <0.1

Cladotanytarsus species C --- 18 (2.89) 15 (4.24) --- --- 39 (1.73)

0.1 <0.1 0.2
151
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv) no. m-2 (cv)
Taxon % % % % % %

Cladotanytarsus species E --- 6 (5.13) --- 5 (5.48) --- ---

<0.1 <0.1

Cladotanytarsus species F --- 3 (3.51) --- --- 38 (3.07) ---

<0.1 0.1

Cladotanytarsus species E or F --- --- --- --- 30 (3.87) ---

<0.1

Cladotanytarsus daviesi 113 (2.49) 544 (2.42) 183 (3.07) 42 (2.96) 445 (3.06) 19 (1.73)
0.3 4.0 0.3 0.1 0.7 0.1

Rheotanytarsus sp. 23 (3.61) 1 (6.25) 21 (3.03) 50 (1.82) 325 (1.92) ---

0.1 <0.1 <0.1 0.2 0.5

Rheotanytarsus sp. A 90 (3.61) --- 150 (3.85) 90 (2.67) 74 (2.02) ---

0.2 0.3 0.3 0.1

Rheotanytarsus pellucidus 31 (3.61) --- --- 11 (3.06) 139 (1.99) ---

0.1 <0.1 0.2

Rheotanytarsus exiguus 299 (2.00) 1 (6.25) 327 (2.68) 296 (1.81) 377 (1.27) 19 (1.73)
0.8 <0.1 0.6 1.0 0.6 0.1

Stempellina sp. --- 14 (3.18) 30 (4.24) 9 (5.48) --- ---

0.1 0.1 <0.1
152
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Stempellina species A 122 (3.61) 855 (1.78) 2,073 (2.71) 72 (2.03) 542 (2.06) 19 (1.73)
0.3 6.3 3.8 0.2 0.9 0.1

Tanytarsus sp. 590 (2.09) 48 (1.70) 376 (1.50) 187 (1.67) 1,598 (0.82) 77 (1.73)
1.5 0.3 0.7 0.6 2.7 0.4

Tanytarsus limneticus --- 5 (6.25) --- --- 37 (3.87) ---

<0.1 0.1

Tanytarsus species A --- 5 (6.25) 98 (3.15) 20 (4.51) 748 (2.37) 19 (1.73)

<0.1 0.2 0.1 1.3 0.1

Tanytarsus species C 3,362 (1.61) 465 (2.14) 11,942 (1.41) 3,252 (1.63) 9,324 (1.32) 232 (1.0)
8.7 3.4 21.6 11.1 15.6 1.3

Tanytarsus species E --- --- --- 20 (5.48) 14 (3.87) ---

0.1 <0.1

Tanytarsus species K --- 5 (4.36) --- --- --- ---

<0.1

Tanytarsus species L 2 (3.61) 39 (3.34) 30 (4.24) 6 (5.48) 223 (1.39) 174 (.33)
<0.1 0.3 0.1 <0.1 0.4 1.0

Tanytarsus species N --- --- --- 4 (5.48) --- ---

<0.1
153
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Tanytarsus species Q --- 1 (6.25) --- 3 (5.48) --- ---

<0.1 <0.1

Tanytarsus species S --- 6 (5.13) 7 (4.24) 4 (5.48) 110 (1.78) ---

<0.1 <0.1 <0.1 0.2

Tanytarsus species T --- --- --- --- 45 (3.21) ---

0.1

Tanytarsus species U --- --- 7 (4.24) --- --- 116 (1.0)

<0.1 0.7

unidentifiable Chironomidae e.i. --- 1 (6.25) 73 (2.47) 29 (3.36) 20 (3.87) ---

<0.1 0.1 0.1 <0.1

Empididae (total) 93 (1.43) 7 (4.36) 84 (0.96) 171 (1.20) 29 (2.43) 44 (0.57)

0.2 <0.1 0.2 0.6 <0.1 0.2

Hemerodromia sp. 93 (1.43) 7 (4.36) 84 (0.96) 169 (1.19) 29 (2.43) 44 (0.57)

0.2 <0.1 0.2 0.6 <0.1 0.2

unidentifiable e.i. Empididae --- --- --- 2 (5.48) --- ---

<0.1

Simuliidae (total) 701 (3.59) 9 (2.29) 472 (3.61) 614 (1.25) 24 (1.85) 29 (1.00)
1.8 0.1 0.9 2.1 <0.1 0.2
154
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Simulium sp. 701 (3.59) 9 (2.29) 469 (3.64) 614 (1.25) 24 (1.85) 29 (1.00)
1.8 0.1 0.8 2.1 <0.1 0.2

Stratiomyidae (total) --- 5 (4.90) 2 (2.96) 2 (5.48) --- ---

<0.1 <0.1 <0.1

Odontomia or Hedriodiscus --- 5 (4.90) 2 (2.96) 2 (5.48) --- ---

<0.1 <0.1 <0.1

Tabanidae (total) --- --- 1 (4.24) --- --- ---

<0.1

Chrysops sp. --- --- 1 (4.24) --- --- ---

<0.1

Tipulidae (total) 2 (3.61) --- 3 (4.24) --- --- ---

<0.1 <0.1

Tipula sp. --- --- 3 (4.24) --- --- ---

<0.1

155
APPENDIX 1 (cont.)
Habitat
Bedrock Sand Cobble/Gravel Snags Tree Roots Debris Packs
-2 -2 -2 -2 -2
no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m (cv) no. m-2 (cv)
Taxon % % % % % %

Mean Total Organisms 38,865 (0.67) 13,665 (0.84) 55,241 (0.88) 29,413 (0.50) 59,802 (0.59) 17,686 (0.47)
100 100 100 100 100 100

Total Taxa 90 121 115 133 123 58

Mean Taxa Richness 27.3 (0.26) 19.9 (0.53) 38.3 (0.10) 35.5 (0.23) 35.0 (0.41) 37.0 (0.08)

Mean Diversity 3.07 (0.14) 2.75 (0.33) 3.49 (0.09) 3.77 (0.16) 3.77 (0.20) 2.97 (0.36)

Mean Eveness 0.65 (0.10) 0.66 (0.19) 0.66 (0.09) 0.74 (0.11) 0.76 (0.11) 0.57 (0.36)

156
APPENDIX 2. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from areally dominant habitats in the Withlacoochee River
study area during 2005-2007.

157
APPENDIX 2A. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the bedrock habitat in the Withlacoochee River study area,
2005-2007.

Rank Taxon No. m-2 (%)

1 Pseudochironomus sp. 7,537 (19.4)

2 Stenelmis sp. 4,536 (11.7)

3 Tanytarsus sp. C 3,362 (8.6)

4 Parakiefferiella sp. 2,259 (5.8)

5 Tvetenia vitracies 1,229 (3.2)

6 Hydroptila sp. 1,192 (3.1)

7 Dicrotenidpes neomodestus 1,123 (2.9)

8 Thiennemanniella sp. B 1,023 (2.6)

9 Bezzia/Palpomyia complex 917 (2.4)

10 Simulium sp. 701 (1.8)

158
APPENDIX 2B. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the cobble/gravel habitat in the Withlacoochee River study area,
2005-2007.

Rank Taxon No. m-2 (%)

1 Tanytarsus sp. C . 11,942 (21.6)

2 Stenelmis sp. 8,783 (15.9)

3 Pseudochironomus sp. 4,965 (9.0)

4 Hydracarina 4,168 (7.5)

5 Polypedilum sp. 2,707 (4.9)

6 Stempellina sp. A 2,073 (3.8)

7 Tricorythodes albilineatus 1,772 (3.2)

8 Hydroptila sp. 1,566 (2.8)

9 Oribatidae 1,432 (2.6)

10 Parakiefferiella sp. 1,130 (2.0)

159
APPENDIX 2C. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the sand habitat in the Withlacoochee River study area, 2005-
2007.

Rank Taxon No. m-2 (%)

1 Corbicula fluminea . 2,042 (14.9)

2 Stempellina sp. A 855 (6.3)

3 Cladotanytarsus daviesi 543 (4.0)

4 Polypedilum halterale 532 (3.9)

5 Pseudochironomus sp. 476 (3.5)

6 Tanytarsus sp. C 465 (3.4)

7 Polypedilum scalaenum 464 (3.4)

8 Stictochironomus sp. 418 (3.1)

9 Probezzia sp. 293 (2.1)

10 Hydroptila sp. 49 (0.4)

160
APPENDIX 2D. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the snag (wood debris) habitat in the Withlacoochee River study
area, 2005-2007.

Rank Taxon No. m-2 (%)

1 Tanytarsus sp. C 3,252 (11.1)

2 Pseudochironomus sp. 3,068 (10.4)

3 Stenelmis sp. 2,904 (9.9)

4 Hydracarina 1,693 (5.8)

5 Xestochironomus sp. 1,520 (5.2)

6 Parakiefferiella sp. 1,281 (4.4)

7 Stenochironomus sp. 1,046 (3.6)

8 Hydroptila sp. 1,000 (3.4)

9 Tvetenia vitracies 966 (3.3)

10 Hyalella azteca. 903 (3.1)

161
APPENDIX 2E. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the tree root system habitat in the Withlacoochee River study
area, 2005-2007.

Rank Taxon No. m-2 (%)

1 Tanytarsus sp. C 9,324 (15.6)

2 Gammarus sp. 2,905 (4.9)

3 Stenelmis sp. 2,299 (3.8)

4 Hyalella azteca 2,196 (3.7)

5 Parakiefferiella sp. 1,870 (3.1)

6 Hydroptila sp. 1,748 (2.9)

7 Hydracarina 1,341 (2.2)

8 Neotrichia sp. 1,078 (1.8)

9 Pentaneura inconspicua 1,024 (1.7)

10 Nectopsyche sp. 759 (1.3)

162
APPENDIX 2F. Density (no. m-2 ) and percent composition (%) of the ten most abundant
invertebrate taxa collected from the organic debris pack habitat in the Withlacoochee River study
area, 2005-2007.

Rank Taxon No. m-2 (%)

1 Corynoneura sp. B 8,884 (50.2)

2 Polypedilum sp. e.i. 1,305 (7.4)

3 Parakiefferiella sp. 522 (3.0)

4 Polypedilum scalaenum 454 (2.6)

5 Xestochironomus sp. 435 (2.5)

6 Corynoneura lobata or sp. G 309 (1.7)

7 Hydracarina 280 (1.6)

8 Tanytarsus sp. C 232 (1.3)

9 Polypedilum illinoense 213 (1.2)

10 Cricotopus bicinctus 174 (1.0)

163
APPENDIX 3. Habitat suitability curves produced for selected descriptors of invertebrate
community quality and indicator invertebrate taxa using the methods of Gore and Judy 1981.

164
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Taxa Richness collected in the
northern Withlacoochee River, Florida, 2005-2007.

165
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Shannon Diversity collected in the
northern Withlacoochee River, Florida, 2005-2007.

166
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168 183 196

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Total Organisms collected in the
northern Withlacoochee River, Florida, 2005-2007.

167
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91
Velocity cm

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Hydracarina (Acari) collected in the
northern Withlacoochee River, Florida, 2005-2007.

168
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168 183 196

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Total Baetidae collected in the
northern Withlacoochee River, Florida, 2005-2007.

169
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Pseudocloeon ephippiatum,
(Ephemeroptera:Baetidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

170
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Total Hydropsychidae (Trichoptera)
collected in the northern Withlacoochee River, Florida, 2005-2007.

171
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Hydropsyche sp.
(Trichoptera:Hydropsychidae) collected in the northern Withlacoochee River, Florida, 2005-
2007.

172
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Hydroptila sp.
(Trichoptera:Hydroptilidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

173
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Corydalus cornutus
(Megaloptera:Corydalidae) collected in the northern Withlacoochee Rive r, Florida, 2005-2007.

174
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Stenelmis sp. (Coleoptera:Elmidae)
collected in the northern Withlacoochee River, Florida, 2005-2007.

175
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122 152

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168 183 196 213

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Total Chironomidae
(Insecta:Diptera) collected in the northern Withlacoochee River, Florida, 2005-2007.

176
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Cricotopus bicinctus
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

177
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122 152 183 213
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168 183 196 213 229 244 257 274

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Parakiefferiella sp.
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

178
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91 122
Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth(cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack
Substrate

Current velocity, depth, and substrate suitability curves for Tvetenia vitracies
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

179
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Pseudochironomus sp.
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

180
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43
Velocity (cm)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Stempellina sp. A
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

181
 1

0.8

0.6

0.4

0.2

0
0 6 12 18 24 30 37 43 49 55 61 67 73 79 85 91

Velocity (cm/sec)

0.8
Suitability

0.6

0.4

0.2

0
15 30 46 61 76 91 107 122 137 152 168 183 196

Depth (cm)

0.8

0.6

0.4

0.2

0
Bedrock Sand Cobble/Gravel Roots Snags Debris Pack

Substrate

Current velocity, depth, and substrate suitability curves for Tanytarsus sp. C
(Diptera:Chironomidae) collected in the northern Withlacoochee River, Florida, 2005-2007.

182
APPENDIX 4. Results of PHABSIM analysis (weighted usable areas) of selected aquatic
invertebrate descriptors of community quality and indicator taxa.

183
 Northern Withlac ooc hee R iver - R eac h 1 - Mac roinvertebrate
C ommunities
140000

120000

100000
WUA (s q ft / 1000 linear ft)

80000

T axa R ichnes s

S hannon
60000 D ivers ity
T otal O rganis ms

40000

20000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

184
 Northern Withlac ooc hee R iver - R eac h 1 - Mites
60000

50000
WUA (s q ft / 1000 linear ft)

40000

30000
H ydracarina
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

185
 Northern Withlac ooc hee R iver - R eac h 1 - E phemeroptera
60000

50000
WUA (s q ft / 1000 linear fett

40000

30000
T otal B aetidae

P s eudocloeon
ephippiatum
20000

10000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

186
 Northern Withlac ooc hee R iver - R eac h 1 - Meg aloptera
20000

18000

16000

14000
WUA (s q ft / 1000 linear ft

12000

10000
C orydalus
8000 cornutus

6000

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

187
 Northern Withlac ooc hee R iver - R eac h 1 - T ric hoptera
80000

70000

60000
WUA (s q ft / 1000 linear ft)

50000

40000 T otal
H ydrops ychidae
H ydrops yche s pp.

30000 H ydroptila s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

188
 Northern Withlac ooc hee R iver - R eac h 1 - C oleoptera
60000

50000
WUA (s q ft / 1000 linear ft)

40000

30000
S tenelmis
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

189
 Northern Withlac ooc hee R iver - R eac h 1 - Diptera I
120000

100000
WUA (s q ft / 1000 linear ft

80000

T otal
60000 C hironomidae
C ricotopus
bicinctus
T anytars us s p. C

40000 P arakiefferiella s p.

20000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

190
 Northern Withlac ooc hee R iver - R eac h 1 - Diptera II
120000

100000
WUA (s q ft / 1000 linear ft)

80000

T otal C hironomidae
60000
P s eudochironomus
s p.
S tempellina s p A.
T vetenia vitracies
40000

20000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

191
 Northern Withlac ooc hee R iver - R eac h 2 - Mac roinvertebrate
C ommunities
120000

100000
WUA (s q ft / 1000 linear ft)

80000

60000 T axa R ichnes s

S hannon
D ivers ity
T otal O rganis ms
40000

20000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

192
 Northern Withlac ooc hee R iver - R eac h 2 - Mites
70000

60000

50000
WUA (s q ft / 1000 linear ft)

40000

H ydracarina
30000
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

193
 Northern Withlac ooc hee R iver - R eac h 2 - E phemeroptera
70000

60000

50000
WUA (s q ft / 1000 linear fett

40000

T otal B aetidae
30000 P s eudocloeon
ephippiatum

20000

10000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

194
 Northern Withlac ooc hee R iver - R eac h 2 - Meg aloptera
45000

40000

35000
WUA (s q ft / 1000 linear ft

30000

25000

20000 C orydalus
cornutus

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

195
 Northern Withlac ooc hee R iver - R eac h 2 - T ric hoptera
70000

60000

50000
WUA (s q ft / 1000 linear ft)

40000

T otal
H ydrops ychidae
30000 H ydrops yche s pp.

H ydroptila s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

196
 Northern Withlac ooc hee R iver - R eac h 2 - C oleoptera
60000

50000
WUA (s q ft / 1000 linear ft)

40000

30000
S tenelmis
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

197
 Northern Withlac ooc hee R iver - R eac h 2 - Diptera I
100000

90000

80000

70000
WUA (s q ft / 1000 linear ft

60000

T otal
50000 C hironomidae
C ricotopus
bicinctus
40000 T anytars us s p. C

P arakiefferiella s p.
30000

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

198
 Northern Withlac ooc hee R iver - R eac h 2- Diptera II
100000

90000

80000

70000
WUA (s q ft / 1000 linear ft)

60000

T otal C hironomidae
50000
P s eudochironomus
s p.
40000 S tempellina s p A.
T vetenia vitracies
30000

20000

10000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

199
 Northern Withlac ooc hee R iver - R eac h 3 - Mac roinvertebrate C ommunities

45000

40000

35000
T axa R ichnes s
WUA (s q ft / 1000 linear ft)

S hannon
30000
D ivers ity
T otal O rganis ms

25000

20000

15000

10000

5000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

200
 Northern Withlac ooc hee R iver - R eac h 3 - Mites

30000

25000
WUA (s q ft / 1000 linear ft)

20000
Hydracarina
s pp.

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

201
 Northern Withlac ooc hee R iver - R eac h 3 - E phemeroptera
30000

25000
WUA (s q ft / 1000 linear fett

T otal B aetidae
20000

P s eudocloeon
ephippiatum

15000

10000

5000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

202
 Northern Withlac ooc hee R iver - R eac h 3 - T ric hoptera

25000

20000
WUA (s q ft / 1000 linear ft)

15000

T otal
Hydrops ychidae
Hydrops yche s pp.
10000
Hydroptila s pp.

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

203
 Northern Withlac ooc hee R iver - R eac h 3 - Meg aloptera

18000

16000

14000

C orydalus
WUA (s q ft / 1000 linear ft

12000
cornutus

10000

8000

6000

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

204
 Northern Withlac ooc hee R iver - R eac h 3 - C oleoptera

30000

25000
WUA (s q ft / 1000 linear ft)

20000 S tenelmis
s pp.

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

205
 Northern Withlac ooc hee R iver - R eac h 3 - Diptera I

35000

30000

25000
WUA (s q ft / 1000 linear ft

20000
T otal
C hironomidae
C ricotopus
15000 bicinctus
T anytars us s p. C
P arakiefferiella s p.

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

206
 Northern Withlac ooc hee R iver - R eac h 3 - Diptera II

35000

30000

25000
WUA (s q ft / 1000 linear ft)

20000
T otal C hironomidae
P s eudochironomus
15000 s p.
S tempellina s p A.
T vetenia vitracies

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

207
 Northern Withlac ooc hee R iver - R eac h 3.5 - Mac roinvertebrate C ommunities

35000

30000

25000
WUA (s q ft / 1000 linear ft)

20000

T axa R ichnes s
S hannon
15000 D ivers ity
T otal O rganis ms

10000

5000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

208
 Northern Withlac ooc hee R iver - R eac h 3.5 - Mites

8000

7000

6000
WUA (s q ft / 1000 linear ft)

5000 Hydracarina
s pp.

4000

3000

2000

1000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

209
 Northern Withlac ooc hee R iver - R eac h 3.5 - E phemeroptera

2500

2000
WUA (s q ft / 1000 linear fett

T otal B aetidae
1500
P s eudocloeon
ephippiatum

1000

500

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

210
 Northern Withlac ooc hee R iver - R eac h 3.5 - T ric hoptera

3500

3000

2500
WUA (s q ft / 1000 linear ft)

2000

T otal
Hydrops ychidae
1500 Hydrops yche s pp.
Hydroptila s pp.

1000

500

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

211
 Northern Withlac ooc hee R iver - R eac h 3.5 - Meg aloptera

700

600

500
WUA (s q ft / 1000 linear ft

400

C orydalus
300
cornutus

200

100

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

212
 Northern Withlac ooc hee R iver - R eac h 3.5 - C oleoptera

10000

9000

8000

7000
WUA (s q ft / 1000 linear ft)

6000

5000
S tenelmis
4000 s pp.

3000

2000

1000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

213
 Northern Withlac ooc hee R iver - R eac h 3.5 - Diptera I

14000

12000

10000
WUA (s q ft / 1000 linear ft

8000
T otal
C hironomidae
C ricotopus
6000 bicinctus
T anytars us s p. C
P arakiefferiella s p.

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

214
 Northern Withlac ooc hee R iver - R eac h 3.5 - Diptera II

14000

12000

10000
WUA (s q ft / 1000 linear ft)

8000
T otal C hironomidae
P s eudochironomus
6000 s p.
S tempellina s p A.
T vetenia vitracies

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

215
 Northern Withlac ooc hee R iver - R eac h 4 - Mac roinvertebrate C ommunities

120000

100000
WUA (s q ft / 1000 linear ft)

80000

60000 T axa R ichnes s

S hannon
D ivers ity
T otal O rganis ms
40000

20000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

216
 Northern Withlac ooc hee R iver - R eac h 4 - Mites

35000

30000

25000
Hydracarina
WUA (s q ft / 1000 linear ft)

s pp.

20000

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

217
 Northern Withlac ooc hee R iver - R eac h 4 - E phemeroptera

40000

35000

30000 T otal B aetidae

WUA (s q ft / 1000 linear fett

P s eudocloeon
ephippiatum
25000

20000

15000

10000

5000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

218
 Northern Withlac ooc hee R iver - R eac h 4 - T ric hoptera

60000

50000
WUA (s q ft / 1000 linear ft)

40000

30000 T otal
Hydrops ychidae
Hydrops yche s pp.
Hydroptila s pp.
20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

219
 Northern Withlac ooc hee R iver - R eac h 4 - Meg aloptera

10000

9000

8000

C orydalus
7000 cornutus
WUA (s q ft / 1000 linear ft

6000

5000

4000

3000

2000

1000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

220
 Northern Withlac ooc hee R iver - R eac h 4 - C oleoptera

45000

40000

35000
WUA (s q ft / 1000 linear ft)

30000

25000

20000 S tenelmis
s pp.

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

221
 Northern Withlac ooc hee R iver - R eac h 4 - Diptera I

80000

70000

60000
WUA (s q ft / 1000 linear ft

50000

T otal
40000 C hironomidae
C ricotopus
bicinctus
T anytars us s p. C
30000
P arakiefferiella s p.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

222
 Northern Withlac ooc hee R iver - R eac h 4 - Diptera II

80000

70000

60000
WUA (s q ft / 1000 linear ft)

50000

T otal C hironomidae
40000
P s eudochironomus
s p.
S tempellina s p A.
30000
T vetenia vitracies

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

223
 Northern Withlac ooc hee R iver - R eac h 5 - Mac roinvertebrate
C ommunities
100000

90000

80000

70000
WUA (s q ft / 1000 linear ft)

60000

50000 T axa R ichnes s

S hannon
40000 D ivers ity
T otal O rganis ms

30000

20000

10000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

224
 Northern Withlac ooc hee R iver - R eac h 5 - Mites
70000

60000

50000
WUA (s q ft / 1000 linear ft)

40000

H ydracarina
30000
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

225
 Northern Withlac ooc hee R iver - R eac h 5 - E phemeroptera
50000

45000

40000

35000
WUA (s q ft / 1000 linear fett

30000

25000
T otal B aetidae

P s eudocloeon
20000
ephippiatum

15000

10000

5000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

226
 Northern Withlac ooc hee R iver - R eac h 5 - Meg aloptera
30000

25000
WUA (s q ft / 1000 linear ft

20000

15000
C orydalus
cornutus

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

227
 Northern Withlac ooc hee R iver - R eac h 5 - T ric hoptera
50000

45000

40000

35000
WUA (s q ft / 1000 linear ft)

30000

25000 T otal
H ydrops ychidae
H ydrops yche s pp.
20000
H ydroptila s pp.

15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

228
 Northern Withlac ooc hee R iver - R eac h 5 - C oleoptera
70000

60000

50000
WUA (s q ft / 1000 linear ft)

40000

S tenelmis
30000
s pp.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

229
 Northern Withlac ooc hee R iver - R eac h 5 - Diptera I
80000

70000

60000
WUA (s q ft / 1000 linear ft

50000

T otal
40000 C hironomidae
C ricotopus
bicinctus
T anytars us s p. C
30000
P arakiefferiella s p.

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

230
 Northern Withlac ooc hee R iver - R eac h 5 - Diptera II
70000

60000

50000
WUA (s q ft / 1000 linear ft)

40000
T otal C hironomidae
P s eudochironomus
30000 s p.
S tempellina s p A.

T vetenia vitracies

20000

10000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

231
 Northern Withlac ooc hee R iver - R eac h 6 - Mac roinvertebrate
C ommunities
35000

30000

25000
WUA (s q ft / 1000 linear ft)

20000

T axa R ichnes s

S hannon
15000 D ivers ity
T otal O rganis ms

10000

5000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

232
 Northern Withlac ooc hee R iver - R eac h 6 - Mites
4500

4000

3500
WUA (s q ft / 1000 linear ft)

3000

2500

2000 H ydracarina
s pp.

1500

1000

500

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

233
 Northern Withlac ooc hee R iver - R eac h 6 - E phemeroptera
6000

5000
WUA (s q ft / 1000 linear fett

4000

3000
T otal B aetidae

P s eudocloeon
ephippiatum
2000

1000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

234
 Northern Withlac ooc hee R iver - R eac h 6 - Meg aloptera
400

350

300
WUA (s q ft / 1000 linear ft

250

200
C orydalus
cornutus
150

100

50

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

235
 Northern Withlac ooc hee R iver - R eac h 6 - T ric hoptera
9000

8000

7000
WUA (s q ft / 1000 linear ft)

6000

5000

T otal
H ydrops ychidae
4000 H ydrops yche s pp.

H ydroptila s pp.
3000

2000

1000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

236
 Northern Withlac ooc hee R iver - R eac h 6 - C oleoptera
10000

9000

8000

7000
WUA (s q ft / 1000 linear ft)

6000

5000
S tenelmis
4000 s pp.

3000

2000

1000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

237
 Northern Withlac ooc hee R iver - R eac h 6 - Diptera I
18000

16000

14000
WUA (s q ft / 1000 linear ft

12000

10000
T otal
C hironomidae
C ricotopus
8000
bicinctus
T anytars us s p. C

6000 P arakiefferiella s p.

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

238
 Northern Withlac ooc hee R iver - R eac h 6 - Diptera II
16000

14000

12000
WUA (s q ft / 1000 linear ft)

10000

T otal C hironomidae
8000
P s eudochironomus
s p.
S tempellina s p A.
6000
T vetenia vitracies

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

239
 Northern Withlac ooc hee R iver - R eac h 7 - Mac roinvertebrate
C ommunities
90000

80000

70000
WUA (s q ft / 1000 linear ft)

60000

50000
T axa R ichnes s

40000 S hannon
D ivers ity
T otal O rganis ms
30000

20000

10000

0
135 150 165 190 220 250 280 310 340 370 400 475 550 650 800 950 1100 1300 2500

Dis c harg e (c fs )

240
 Northern Withlac ooc hee R iver - R eac h 7 - Mites
16000

14000

12000
WUA (s q ft / 1000 linear ft)

10000

8000
H ydracarina
s pp.
6000

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

241
 Northern Withlac ooc hee R iver - R eac h 7 - E phemeroptera
14000

12000

10000
WUA (s q ft / 1000 linear fett

8000

T otal B aetidae
6000 P s eudocloeon
ephippiatum

4000

2000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
1500
2500
Dis c harg e (c fs )

242
 Northern Withlac ooc hee R iver - R eac h 7 - Meg aloptera
1200

1000
WUA (s q ft / 1000 linear ft

800

600
C orydalus
cornutus

400

200

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

243
 Northern Withlac ooc hee R iver - R eac h 7 - T ric hoptera
25000

20000
WUA (s q ft / 1000 linear ft)

15000

T otal
H ydrops ychidae
H ydrops yche s pp.
10000
H ydroptila s pp.

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

244
 Northern Withlac ooc hee R iver - R eac h 7 - C oleoptera
18000

16000

14000
WUA (s q ft / 1000 linear ft)

12000

10000

8000 S tenelmis
s pp.

6000

4000

2000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

245
 Northern Withlac ooc hee R iver - R eac h 7 - Diptera I
50000

45000

40000

35000
WUA (s q ft / 1000 linear ft

30000

T otal
25000 C hironomidae
C ricotopus
bicinctus
20000 T anytars us s p. C

P arakiefferiella s p.
15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

246
 Northern Withlac ooc hee R iver - R eac h 7 - Diptera II
50000

45000

40000

35000
WUA (s q ft / 1000 linear ft)

30000

T otal C hironomidae
25000
P s eudochironomus
s p.
20000 S tempellina s p A.

T vetenia vitracies
15000

10000

5000

0
5
5
5
5
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
00
00
00
00
00
13
14
15
16
18
20
22
24
26
28
30
32
34
36
38
40
45
50
55
60
70
80
90
10
11
12
15
25
Dis c harg e (c fs )

247
APPENDIX 5. Time series analyses of aquatic invertebrate community descriptor and indicator
taxa PHABSIM results.

248
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 1

70%

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%
April

August
May

July
January

February

November
September

October

December
June
March

249
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 1

120%

100%

80%
Habitat Gain / Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

250
 Shannon Diversity (1940-1969)
Northern Withlacoochee River - Reach 1
80%

70%

60%

50%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%
April

August
May

July
January

February

November
September

October

December
June
March

251
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 1

120%

100%

80%
Habitat GAin / Loss

60%
10% Flow Reduction
20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

252
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 1

40%

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

253
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 1

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

254
 Hydracarina sp. 1940-1969
Northern Withlacoochee River Reach 1

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction
30%
40% Flow Reduction

20%

10%

0%

-10%
April

August
May

July
February
January

November
March

June

October
September

December
255
 Hydracarina sp. 1970-1999
Northern Withlacoochee River Reach 1

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

256 December
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 1

40%

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

0% 20% Flow Reduction
30% Flow Reduction

-10% 40% Flow Reduction

-20%

-30%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

257
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 1

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction
20% 30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

258
 Pseudocloeon ephippiatum (1940-1969)
Withlacoochee River - Reach 1

100%

80%

60%
Habitat Gain/Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

259
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 1

140%

120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

60%
20% Flow Reduction
30% Flow Reduction
40%
40% Flow Reduction

20%

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

260
 Corydalus cornutus 1940-1969
Northern Withlacoochee river Reach 1
120%

100%

80%
Habitat Gain / Loss

60%

40%
10% Flow Reduction
20% Flow Reduction

20% 30% Flow Reduction

40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
261
 Corydalus cornutus 1970-1999
Northern Withlacoochee River Reach 1

180%

160%

140%

120%

100%
Habitat Gain / Loss

10% Flow Reduction

80%
20% Flow Reduction
30% Flow Reduction
60%
40% Flow Reduction
40%

20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

262 December
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 1

50%

40%

30%

20%
Habitat Gain/Loss

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-10%

-20%

-30%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

263
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 1
100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

264
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 1

50%

40%

30%

20%
Habitat Gain/Loss

10%
10% Flow Reduction
20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%

-40%

-50%
April

August
May

July
February
January

November
March

June

October
September

December
265
 Hydropsyche spp. (1970-1999)
Withlacoochee River - Reach 1

100%

80%

60%
Habitat Gain/Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

266
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 1
60%

50%

40%

30%
Habitat Gain / Loss

20%

10%
10% Flow Reduction
20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
267
 Hydroptila sp. 1970-1999
Northern Withlacoochee River Reach 1

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

268 December
 Stenelmis sp. 1940-1969
Northern Withlacoochee River Reach 1
120%

100%

80%
Habitat Gain / Loss

60%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
269
 Stenelmis sp. 1970-1999
Northern Withlacoochee River Reach 1

160%

140%

120%

100%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
80%
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

270 December
 Total Chironomidae 1940-1969
Northern Withlacoochee River - Reach 1

30%

20%

10%
Habitat G ain / L os s

0%
10% F low
R eduction
-10% 20% F low
R eduction
30% F low
R eduction
-20% 40% F low
R eduction

-30%

-40% A ug us t
A pril

November
Oc tober

Dec ember
S eptember
J une

J uly
J anuary

F ebruary

May
Marc h

271
 Total Chironomidae 1970-1999
Northern Withlacoochee River Reach 1

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

272 December
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River Reach 1

0%

-5%
Habitat Gain / Loss

-10%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
-15%

-20%

-25%
April

August
May

July
February
January

November
March

June

October
September

December
273
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River Reach 1

0%

-5%
Habitat Gain / Loss

-10%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
-15%

-20%

-25%
April

August
May

July
February
January

November
March

June

October
September

274 December
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River Reach 1

40%

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
275
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River Reach 1

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

276 December
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River Reach 1

15%

10%

5%

0%
Habitat Gain / Loss

-5%
10% Flow Reduction
20% Flow Reduction
-10%
30% Flow Reduction
40% Flow Reduction
-15%

-20%

-25%

-30%

-35%
April

August
May

July
February
January

November
March

June

October
September

December
277
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River Reach 1

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-10%

-20%

-30%

-40%
April

August
May

July
February
January

November
March

June

October
September

278 December
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River Reach 1

30%

20%

10%

0%

-10%
Habitat Gain / Loss

10% Flow Reduction

-20%
20% Flow Reduction
30% Flow Reduction
-30%
40% Flow Reduction

-40%

-50%

-60%

-70%

-80%
April

August
May

July
February
January

November
March

June

October
September

December
279
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River Reach 1

80%

60%

40%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction
-20%

-40%

-60%

-80%
April

August
May

July
February
January

November
March

June

October
September

280 December
 Stempellina sp. A 1940-1969
Northern Withlacoochee River Reach 1

600%

500%

400%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
300%
30% Flow Reduction
40% Flow Reduction

200%

100%

0%
April

August
May

July
February
January

November
March

June

October
September

December
281
 Stempellina sp. A 1970-1999
Northern Withlacoochee River Reach 1

700%

600%

500%
Habitat Gain / Loss

400% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
300% 40% Flow Reduction

200%

100%

0%
April

August
May

July
February
January

November
March

June

October
September

282 December
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River Reach 1

60%

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-10%

-20%

-30%

-40%

-50%
April

August
May

July
February
January

November
March

June

October
September

December
283
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River Reach 1

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
0%

-20%

-40%

-60%
April

August
May

July
February
January

November
March

June

October
September

284 December
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 2

80%

70%

60%

50%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Reduction
30% Flow Reduction
30%
40% Flow Reduction

20%

10%

0%

-10%
April

August
May

July
January

February

November
September

October

December
June
March

285
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 2

120%

100%

80%
Habitat Gain / Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

286
 Shannon Diversity 1940-1969
Northern Withlacoochee River Reach 2
90%

80%

70%

60%
Habitat Gain / Loss

50%

40%

10% Flow Reduction

30%
20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

10%

0%

-10%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

287
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 2

140%

120%

100%

80%
Habitat GAin / Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction

40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

288
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 2

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
April

August
May

July
January

February

November
September

October

December
June
March

289
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 2

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

290
 Hydracarina sp. 1940-1969
Northern Withlacoochee River Reach 2

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
291
 Hydracarina sp. 1970-1999
Northern Withlacoochee River Reach 2

180%

160%

140%

120%
Habitat Gain / Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

292 December
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 2

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

10% 20% Flow Reduction
30% Flow Reduction

0% 40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
January

February

November
September

October

December
June
March

293
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 2

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction
20% 30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

294
 Pseudocloeon ephippiatum (1940-1969)
Withlacoochee River - Reach 2

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

295
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 2

140%

120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction
40%

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

296
 Corydalus cornutus 1940-1969
Northern Withlacoochee river Reach 2
140%

120%

100%
Habitat Gain / Loss

80%

60%
10% Flow Reduction
20% Flow Reduction

40% 30% Flow Reduction

40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
297
 Corydalus cornutus 1970-1999
Northern Withlacoochee River Reach 2

200%

180%

160%

140%
Habitat Gain / Loss

120%
10% Flow Reduction
20% Flow Reduction
100%
30% Flow Reduction
40% Flow Reduction
80%

60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

298 December
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 2

80%

60%

40%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

299
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 2
120%

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction

20%

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

300
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 2

80%

60%

40%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
301
 Hydropsyche spp. (1970-1999)
Withlacoochee River - Reach 2

120%

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

302
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 2
70%

60%

50%

40%
Habitat Gain / Loss

30%

20%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
303
 Hydroptila sp. 1970-1999
Northern Withlacoochee River Reach 2

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

304 December
 Stenelmis sp. 1940-1969
Northern Withlacoochee River Reach 2
140%

120%

100%
Habitat Gain / Loss

80%

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
305
 Stenelmis sp. 1970-1999
Northern Withlacoochee River Reach 2

180%

160%

140%

120%
Habitat Gain / Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

306 December
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 2

40%

30%

20%
Habitat G ain / L os s

10%

0% 10% F low
R eduction
20% F low
R eduction
-10% 30% F low
R eduction
40% F low
R eduction
-20%

-30%

-40% A ug us t
A pril

November
Oc tober

Dec ember
S eptember
J une

J uly
J anuary

F ebruary

May
Marc h

307
 Total Chironomidae 1970-1999
Northern Withlacoochee River Reach 2

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

308 December
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River Reach 2

20%

15%

10%

5%
Habitat Gain / Loss

10% Flow Reduction

0%
20% Flow Reduction
30% Flow Reduction
-5%
40% Flow Reduction

-10%

-15%

-20%

-25%
April

August
May

July
February
January

November
March

June

October
September

December
309
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River Reach 2

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

310 December
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River Reach 2

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
311
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River Reach 2

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

312 December
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River Reach 2

30%

20%

10%
Habitat Gain / Loss

0% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
-10% 40% Flow Reduction

-20%

-30%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
313
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River Reach 2

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

314 December
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River Reach 2

30%

20%

10%

0%
Habitat Gain / Loss

-10%
10% Flow Reduction
20% Flow Reduction
-20%
30% Flow Reduction
40% Flow Reduction
-30%

-40%

-50%

-60%

-70%
April

August
May

July
February
January

November
March

June

October
September

December
315
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River Reach 2

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-20%

-40%

-60%

-80%
April

August
May

July
February
January

November
March

June

October
September

December
316
 Stempellina sp. A 1940-1969
Northern Withlacoochee River Reach 2

600%

500%

400%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
300%
30% Flow Reduction
40% Flow Reduction

200%

100%

0%
April

August
May

July
February
January

November
March

June

October
September

December
317
 Stempellina sp. A 1970-1999
Northern Withlacoochee River Reach 2

700%

600%

500%
Habitat Gain / Loss

400% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
300% 40% Flow Reduction

200%

100%

0%
April

August
May

July
February
January

November
March

June

October
September

318 December
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River Reach 2

60%

50%

40%

30%
Habitat Gain / Loss

20%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
319
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River Reach 2

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

320 December
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 3

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

321
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 3

70%

60%

50%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
322
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 3

120%

100%

80%
Habitat GAin / Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
323
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 3
70%

60%

50%
Habitat Gain / Loss

40%

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
324
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 3

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
325
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 3

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December
326
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 3

180%

160%

140%

120%
Habitat Gain / Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

327
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 3

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

328
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 3

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

20% 30% Flow Reduction

40% Flow Reduction

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
329
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 3

50%

40%

30%

20%
Habitat Gain / Loss

10%
10% Flow Reduction
20% Flow Reduction
0% 30% Flow Reduction
40% Flow Reduction

-10%

-20%

-30%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
330
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 3

200%

150%
Habitat Gain/Loss

100%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
50%

0%

-50%
August
July
April

May

June

October
March
January

February

November
September

December
331
 Pseudocloeon ephippiatum (1940-1969)
Withlacoochee River - Reach 3

140%

120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November

December
September

332
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 3
120%

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction

20%

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
333
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 3

80%

70%

60%

50%

40%
Habitat Gain/Loss

10% Flow Reduction

30%
20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

10%

0%

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December
334
 Hydropsyche spp. (1970-1999)
Northern Withlacoochee River - Reach 3

120%

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
335
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 3

60%

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
10%
40% Flow Reduction

0%

-10%

-20%

-30%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

336
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 3

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

337
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 3
70%

60%

50%

40%
Habitat Gain / Loss

30%

20%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

338
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 3

300%

250%

200%
Habitat Gain / Loss

10% Flow Reduction

150%
20% Flow Reduction
30% Flow Reduction
100%
40% Flow Reduction

50%

0%

-50%
August
July

October
April

May

June
January

March

November
February

De cem ber
Septem ber

339
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 3
200%

150%
Habitat Gain / Loss

100%

10% Flow Reduction

20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction

0%

-50%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

340
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 3

180%

160%

140%

120%
Habitat Gain / Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

341
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 3
140%

120%

100%
Habitat Gain / Loss

80%

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

342
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 3

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

343
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 3

40%

30%

20%
H abitat G ain / L os s

10%

0% 10% F low
R educ tion
20% F low
R educ tion
-10% 30% F low
R educ tion
40% F low
-20% R educ tion

-30%

-40% A ug us t
A pril

Oc tober
S eptember

November

D ec ember
J une
Marc h

May
J anuary

F ebruary

J uly

344
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 3

30%

25%

20%

15%
Habitat Gain / Loss

10%
10% Flow Reduction
20% Flow Reduction
5%
30% Flow Reduction
40% Flow Reduction
0%

-5%

-10%

-15%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

345
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 3

15%

10%

5%
Habitat Gain / Loss

0% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
-5% 40% Flow Reduction

-10%

-15%

-20%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

346
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 3

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

347
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 3

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
August
July

Oct ober
April
January

June
May

Nov ember
March
February

December
Septem ber

348
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 3

70%

60%

50%

40%
Habitat Gain / Loss

30%
10% Flow Reduction
20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%

-30%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

349
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 3

20%

15%

10%

5%
Habitat Gain / Loss

0%
10% Flow Reduction
20% Flow Reduction
-5%
30% Flow Reduction
40% Flow Reduction
-10%

-15%

-20%

-25%

-30%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

350
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 3

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-20%

-40%

-60%

-80%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

351
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 3

30%

20%

10%

0%
Habitat Gain / Loss

-10%
10% Flow Reduction
20% Flow Reduction
-20%
30% Flow Reduction
40% Flow Reduction
-30%

-40%

-50%

-60%

-70%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

352
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 3

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

353
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 3

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

354
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 3

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

355
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 3

70%

60%

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
10%
40% Flow Reduction

0%

-10%

-20%

-30%

-40%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

356
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

357
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 3.5

70%

60%

50%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
358
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat GAin / Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
359
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 3.5
70%

60%

50%
Habitat Gain / Loss

40%

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
360
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 3.5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
361
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 3.5

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
362
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

363
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 3.5

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

364
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 3.5

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

20% 30% Flow Reduction

40% Flow Reduction

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
365
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 3.5

40%

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

0% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
366
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 3.5

250%

200%

150%
Habitat Gain/Loss

100% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
50% 40% Flow Reduction

0%

-50%

-100%
August
July
April

May

June

October
March
January

February

November

December
September

367
 Pseudocloeon ephippiatum (1940-1969)
Northern Withlacoochee River - Reach 3.5

140%

120%

100%

80%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%

-60%
August
July
April

May

June

October
March
January

February

November
September

December
368
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 3.5
120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

60%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
369
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 3.5

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
370
 Hydropsyche spp. (1970-1999)
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
371
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 3.5

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

372
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

373
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 3.5
40%

30%

20%
Habitat Gain / Loss

10%

10% Flow Reduction

0% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
-10%

-20%

-30%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

374
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 3.5

800%

700%

600%

500%
Habitat Gain / Loss

400%
10% Flow Reduction
20% Flow Reduction
300%
30% Flow Reduction
40% Flow Reduction
200%

100%

0%

-100%

-200%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

375
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 3.5
300%

250%

200%
Habitat Gain / Loss

150%

100%
10% Flow Reduction
20% Flow Reduction

50% 30% Flow Reduction

40% Flow Reduction

0%

-50%

-100%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

376
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

377
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 3.5
80%

70%

60%
Habitat Gain / Loss

50%

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

378
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 3.5

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

379
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 3.5

40%

30%

20%
H abitat G ain / L os s

10%

0% 10% F low
R educ tion
20% F low
R educ tion
-10% 30% F low
R educ tion
40% F low
-20% R educ tion

-30%

-40% A ug us t
A pril

Oc tober
S eptember

November

D ec ember
J une
Marc h

May
J anuary

F ebruary

J uly

380
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 3.5

20%

15%

10%
Habitat Gain / Loss

5% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-5%

-10%

-15%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

381
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 3.5

15%

10%

5%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction

-5%

-10%

-15%
August
July

Oct ober
April
January

June
May

Nov ember
March
February

December
Septem ber

382
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 3.5

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

383
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 3.5

20%

15%

10%

5%
Habitat Gain / Loss

0%
10% Flow Reduction
20% Flow Reduction
-5%
30% Flow Reduction
40% Flow Reduction
-10%

-15%

-20%

-25%

-30%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

384
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

40%

30%

20%
Habitat Gain / Loss

10% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

385
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 3.5

20%

15%

10%

5%
Habitat Gain / Loss

10% Flow Reduction

0%
20% Flow Reduction
30% Flow Reduction
-5% 40% Flow Reduction

-10%

-15%

-20%

-25%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

386
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
0%

-20%

-40%

-60%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

387
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 3.5

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
0%

-20%

-40%

-60%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

388
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 3.5

20%

10%

0%
Habitat Gain / Loss

-10% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
-20% 40% Flow Reduction

-30%

-40%

-50%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

389
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 3.5

200%

180%

160%

140%
Habitat Gain / Loss

120%
10% Flow Reduction
20% Flow Reduction
100%
30% Flow Reduction
40% Flow Reduction
80%

60%

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

390
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 3.5

250%

200%
Habitat Gain / Loss

150%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
100%

50%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

391
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 3.5

120%

100%

80%
Habitat Gain / Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

392
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 3.5

70%

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30%
20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%

-20%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

393
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 4

100%

90%

80%

70%
Habitat Gain / Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
394
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 4

50%

45%

40%

35%
Habitat Gain / Loss

30%
10% Flow Reduction
20% Reduction
25%
30% Flow Reduction
40% Flow Reduction
20%

15%

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December
395
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 4

100%

90%

80%

70%

60%
Habitat GAin / Loss

10% Flow Reduction

50% 20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

396
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 4
60%

50%

40%
Habitat Gain / Loss

30%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
397
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
398
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 4

35%

30%

25%

20%
Habitat Gain / Loss

15%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%

-15%
August
July
April

May

June

October
March
January

February

November
September

December
399
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 4

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

400
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 4

70%

60%

50%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

401
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

20% 30% Flow Reduction

40% Flow Reduction

0%

-20%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
402
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 4

30%

25%

20%

15%

10%
Habitat Gain / Loss

10% Flow Reduction

5% 20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-5%

-10%

-15%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
403
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
404
 Pseudocloeon ephippiatum (1940-1969)
Northern Withlacoochee River - Reach 4

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
405
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 4
100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
406
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 4

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
407
 Hydropsyche spp. (1970-1999)
Northern Withlacoochee River - Reach 4

120%

100%

80%
Habitat Gain/Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
408
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 4

60%

50%

40%
Habitat Gain/Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

409
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

410
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 4
35%

30%

25%

20%
Habitat Gain / Loss

15%

10%
10% Flow Reduction
20% Flow Reduction
5%
30% Flow Reduction
40% Flow Reduction
0%

-5%

-10%

-15%
August
July

Oct ober
April

May

June
January

Nov ember
March
February

December
Septem ber

411
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

412
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 4
60%

50%

40%
Habitat Gain / Loss

30%

20%
10% Flow Reduction
20% Flow Reduction

10% 30% Flow Reduction

40% Flow Reduction

0%

-10%

-20%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

413
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 4

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

414
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 4
80%

70%

60%
Habitat Gain / Loss

50%

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

415
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

416
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 4

30%

25%

20%

15%
H abitat G ain / L os s

10%

5% 10% F low
R educ tion
20% F low
0% R educ tion
30% F low
R educ tion
-5% 40% F low
R educ tion
-10%

-15%

-20% A ug us t
A pril

Oc tober
S eptember

November

D ec ember
J une
Marc h

May
J anuary

F ebruary

J uly

417
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 4

40%

35%

30%

25%

20%
Habitat Gain / Loss

10% Flow Reduction

15%
20% Flow Reduction
30% Flow Reduction
10%
40% Flow Reduction

5%

0%

-5%

-10%

-15%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

418
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 4

20%

15%

10%
Habitat Gain / Loss

5% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-5%

-10%

-15%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

419
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

420
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 4

30%

25%

20%

15%
Habitat Gain / Loss

10%
10% Flow Reduction
20% Flow Reduction
5%
30% Flow Reduction
40% Flow Reduction
0%

-5%

-10%

-15%

-20%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

421
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 4

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction

-10%

-20%

-30%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

422
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 4

15%

10%

5%

0%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
-5%
30% Flow Reduction
40% Flow Reduction
-10%

-15%

-20%

-25%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

423
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 4

30%

20%

10%

0%
Habitat Gain / Loss

10% Flow Reduction

-10%
20% Flow Reduction
30% Flow Reduction
-20%
40% Flow Reduction

-30%

-40%

-50%

-60%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

424
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 4

10%

0%

-10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
-20%
30% Flow Reduction
40% Flow Reduction

-30%

-40%

-50%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

425
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 4

450%

400%

350%

300%
Habitat Gain / Loss

10% Flow Reduction

250%
20% Flow Reduction
30% Flow Reduction
200%
40% Flow Reduction

150%

100%

50%

0%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

426
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 4

400%

350%

300%

250%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
200%
30% Flow Reduction
40% Flow Reduction
150%

100%

50%

0%
August
July

Oct ober
April
January

June
May

Nov ember
March
February

December
Septem ber

427
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 4

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July

Oct ober
April

May
January

June

Nov ember
March
February

December
Septem ber

428
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 4

35%

30%

25%

20%

15%
Habitat Gain / Loss

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction
5%
40% Flow Reduction

0%

-5%

-10%

-15%

-20%
August
July

Oct ober
April
January

May

June

Nov ember
March
February

December
Septem ber

429
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 5

45%

40%

35%

30%
Habitat Gain / Loss

25% 10% Flow Reduction

20% Reduction
30% Flow Reduction
20%
40% Flow Reduction

15%

10%

5%

0%
April

August
May

July
January

February

November
September

October

December
June
March

430
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 5

100%

90%

80%

70%
Habitat Gain / Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

431
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 5
50%

45%

40%

35%
Habitat Gain / Loss

30%

25%
10% Flow Reduction
20% Flow Reduction
20% 30% Flow Reduction
40% Flow Reduction
15%

10%

5%

0%
April

August
May

July
January

February

November
September

October

December
June
March

432
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 5

100%

90%

80%

70%

60%
Habitat GAin / Loss

10% Flow Reduction

50% 20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

433
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 5

35%

30%

25%

20%
Habitat Gain / Loss

15%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%

-15%
April

August
May

July
January

February

November
September

October

December
June
March

434
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

435
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 5

70%

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%
April

August
May

July
February
January

November
March

June

October
September

December
436
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 5

120%

100%

80%
Habitat Gain / Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

437 December
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 5

50%

40%

30%
Habitat Gain / Loss

20%
10% Flow Reduction
20% Flow Reduction
10% 30% Flow Reduction
40% Flow Reduction

0%

-10%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

438
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

439
 Pseudocloeon ephippiatum (1940-1969)
Northern Withlacoochee River - Reach 5

45%

40%

35%

30%
Habitat Gain/Loss

25%
10% Flow Reduction
20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
15%

10%

5%

0%

-5%
April

August
May

July
January

February

November
September

October

December
June
March

440
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

441
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 5
50%

40%

30%
Habitat Gain / Loss

20%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%
April

August
May

July
February
January

November
March

June

October
September

December
442
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

443 December
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 5

45%

40%

35%

30%
Habitat Gain/Loss

10% Flow Reduction

25%
20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

15%

10%

5%

0%
April

August
May

July
January

February

November
September

October

December
June
March

444
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 5
100%

90%

80%

70%
Habitat Gain/Loss

60% 10% Flow Reduction

20% Flow Reduction
50% 30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

445
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 5

45%

40%

35%

30%
Habitat Gain/Loss

25%
10% Flow Reduction
20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
15%

10%

5%

0%

-5%
April

August
May

July
February
January

November
March

June

October
September

December
446
 Hydropsyche spp. (1970-1999)
Northern Withlacoochee River - Reach 5

100%

90%

80%

70%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

447
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 5
40%

35%

30%

25%
Habitat Gain / Loss

20%

15%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%
April

August
May

July
February
January

November
March

June

October
September

December
448
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

449 December
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 5
80%

70%

60%
Habitat Gain / Loss

50%

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
April

August
May

July
February
January

November
March

June

October
September

December
450
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

451 December
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 5

40%

30%

20%
Habitat G ain / L os s

10% 10% F low

R eduction
20% F low
R eduction
30% F low
0% R eduction
40% F low
R eduction

-10%

-20% A ug us t
A pril

November
Oc tober

Dec ember
S eptember
J une

J uly
J anuary

F ebruary

May
Marc h

452
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

453 December
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 5

20%

15%

10%
Habitat Gain / Loss

5% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-5%

-10%

-15%
April

August
May

July
February
January

November
March

June

October
September

December
454
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 5

50%

40%

30%
Habitat Gain / Loss

20% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%

-20%
April

August
May

July
February
January

November
March

June

October
September

455 December
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 5

25%

20%

15%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
5%
30% Flow Reduction
40% Flow Reduction
0%

-5%

-10%

-15%
April

August
May

July
February
January

November
March

June

October
September

December
456
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 5

50%

40%

30%
Habitat Gain / Loss

20% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%

-20%
April

August
May

July
February
January

November
March

June

October
September

457 December
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 5

15%

10%

5%

0%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
-5%
30% Flow Reduction
40% Flow Reduction
-10%

-15%

-20%

-25%
April

August
May

July
February
January

November
March

June

October
September

December
458
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 5

40%

30%

20%
Habitat Gain / Loss

10% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

459 December
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 5

20%

10%

0%

-10%
Habitat Gain / Loss

10% Flow Reduction

-20%
20% Flow Reduction
30% Flow Reduction
-30%
40% Flow Reduction

-40%

-50%

-60%

-70%
April

August
May

July
February
January

November
March

June

October
September

December
460
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 5

100%

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
0%
40% Flow Reduction

-20%

-40%

-60%

-80%
April

August
May

July
February
January

November
March

June

October
September

December
461
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 5

300%

250%

200%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
150%
30% Flow Reduction
40% Flow Reduction

100%

50%

0%
April

August
May

July
February
January

November
March

June

October
September

December
462
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 5

350%

300%

250%
Habitat Gain / Loss

200% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
150% 40% Flow Reduction

100%

50%

0%
April

August
May

July
February
January

November
March

June

October
September

463 December
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 5

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction

0%

-10%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
464
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 5

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

465 December
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 6

90%

80%

70%

60%
Habitat Gain / Loss

50% 10% Flow Reduction

20% Reduction
30% Flow Reduction
40%
40% Flow Reduction

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

466
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 6

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

467
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 6
90%

80%

70%

60%
Habitat Gain / Loss

50%

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

468
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 6

140%

120%

100%
Habitat GAin / Loss

80%
10% Flow Reduction
20% Flow Reduction
60% 30% Flow Reduction
40% Flow Reduction

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

469
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 6

50%

40%

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%
April

August
May

July
January

February

November
September

October

December
June
March

470
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 6

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

471
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 6

90%

80%

70%

60%
Habitat Gain / Loss

10% Flow Reduction

50%
20% Flow Reduction
30% Flow Reduction
40%
40% Flow Reduction

30%

20%

10%

0%
April

August
May

July
February
January

November
March

June

October
September

December
472
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 6

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
473
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 6

40%

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

0% 20% Flow Reduction
30% Flow Reduction

-10% 40% Flow Reduction

-20%

-30%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

474
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 6

100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction
20% 30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

475
 Pseudocloeon ephippiatum (1940-1969)
Withlacoochee River - Reach 6

80%

70%

60%

50%
Habitat Gain/Loss

40%
10% Flow Reduction
20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

476
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 6

140%

120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction
40%

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

477
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 6
100%

80%

60%
Habitat Gain / Loss

40%

10% Flow Reduction

20% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
478
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 6

160%

140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction
40%

20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
479
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 6

80%

70%

60%

50%
Habitat Gain/Loss

40%
10% Flow Reduction
20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

480
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 6
120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

60%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

481
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 6

80%

70%

60%

50%
Habitat Gain/Loss

40%
10% Flow Reduction
20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction
20%

10%

0%

-10%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
482
 Hydropsyche spp. (1970-1999)
Northern Withlacoochee River - Reach 6

120%

100%

80%
Habitat Gain/Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

483
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 6
60%

50%

40%

30%
Habitat Gain / Loss

20%

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
484
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 6

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
485
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 6
100%

90%

80%

70%
Habitat Gain / Loss

60%

50%
10% Flow Reduction
20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
April

August
May

July
February
January

November
March

June

October
September

December
486
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 6

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
487
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 6

40%

30%

20%
Habitat G ain / L os s

10%

0% 10% F low
R eduction
20% F low
R eduction
-10% 30% F low
R eduction
40% F low
R eduction
-20%

-30%

-40% A ug us t
A pril

November
Oc tober

Dec ember
S eptember
J une

J uly
J anuary

F ebruary

May
Marc h

488
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 6

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
489
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 6

4%

2%

0%

-2%
Habitat Gain / Loss

-4%
10% Flow Reduction
20% Flow Reduction
-6%
30% Flow Reduction
40% Flow Reduction
-8%

-10%

-12%

-14%

-16%
April

August
May

July
February
January

November
March

June

October
September

December
490
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 6

10%

5%
Habitat Gain / Loss

0%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
-5%

-10%

-15%
April

August
May

July
February
January

November
March

June

October
September

December
491
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 6

40%

30%

20%
Habitat Gain / Loss

10% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
492
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 6

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
493
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 6

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
494
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 6

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
495
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 6

30%

20%

10%

0%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
-10%
30% Flow Reduction
40% Flow Reduction
-20%

-30%

-40%

-50%
April

August
May

July
February
January

November
March

June

October
September

December
496
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 6

80%

60%

40%
Habitat Gain / Loss

20% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-20%

-40%

-60%
April

August
May

July
February
January

November
March

June

October
September

December
497
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 6

300%

250%

200%
Habitat Gain / Loss

150% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
100% 40% Flow Reduction

50%

0%

-50%
April

August
May

July
February
January

November
March

June

October
September

December
498
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 6

600%

500%

400%
Habitat Gain / Loss

300% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
200% 40% Flow Reduction

100%

0%

-100%
April

August
May

July
February
January

November
March

June

October
September

December
499
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 6

80%

60%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
500
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 6

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
501
 Taxa Richness (1940-1969)
Northern Withlacoochee River - Reach 7

90%

80%

70%

60%
Habitat Gain / Loss

50% 10% Flow Reduction

20% Reduction
30% Flow Reduction
40%
40% Flow Reduction

30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

502
 Taxa Richness (1970-1999)
Northern Withlacoochee River - Reach 7

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

503
 Shannon Diversity 1940-1969
Northern Withlacoochee River - Reach 7
100%

90%

80%

70%
Habitat Gain / Loss

60%

50%
10% Flow Reduction
20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
April

August
May

July
January

February

November
September

October

December
June
March

504
 Shannon Diversity 1970-1999
Northern Withlacoochee River - Reach 7

140%

120%

100%
Habitat GAin / Loss

80%
10% Flow Reduction
20% Flow Reduction
60% 30% Flow Reduction
40% Flow Reduction

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

505
 Total Organisms (1940-1969)
Northern Withlacoochee River - Reach 7

70%

60%

50%

40%
Habitat Gain / Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

10%

0%

-10%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

506
 Total Organisms (1970-1999)
Northern Withlacoochee River - Reach 7

120%

100%

80%
Habitat Gain / Loss

60% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% 40% Flow Reduction

20%

0%

-20%
April

August
May

July
January

February

November
September

October

December
June
March

507
 Hydracarina sp. 1940-1969
Northern Withlacoochee River - Reach 7

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
508
 Hydracarina sp. 1970-1999
Northern Withlacoochee River - Reach 7

140%

120%

100%
Habitat Gain / Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

509 December
 Total Baetidae (1940-1969)
Northern Withlacoochee River - Reach 7

70%

60%

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% 20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%

-20%

-30%
April

August
May

July
January

February

November
September

October

December
June
March

510
 Total Baetidae (1970-1999)
Northern Withlacoochee River - Reach 7

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction

20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
January

February

November
September

October

December
June
March

511
 Pseudocloeon ephippiatum (1940-1969)
Withlacoochee River - Reach 7

140%

120%

100%
Habitat Gain/Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

512
 Pseudocloeon ephippiatum (1970-1999)
Northern Withlacoochee River - Reach 7

250%

200%
Habitat Gain/Loss

150%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
100%

50%

0%
April

August
May

July
January

February

November
September

October

December
June
March

513
 Corydalus cornutus 1940-1969
Northern Withlacoochee River - Reach 7
250%

200%

150%
Habitat Gain / Loss

100%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
50% 40% Flow Reduction

0%

-50%
April

August
May

July
February
January

November
March

June

October
September

December
514
 Corydalus cornutus 1970-1999
Northern Withlacoochee River - Reach 7

500%

450%

400%

350%
Habitat Gain / Loss

300%
10% Flow Reduction
20% Flow Reduction
250%
30% Flow Reduction
40% Flow Reduction
200%

150%

100%

50%

0%
April

August
May

July
February
January

November
March

June

October
September

515 December
 Total Hydropsychidae (1940-1969)
Northern Withlacoochee River - Reach 7

180%

160%

140%

120%
Habitat Gain/Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
April

August
May

July
January

February

November
September

October

December
June
March

516
 Total Hydropsychidae (1970-1999)
Northern Withlacoochee River - Reach 7
300%

250%

200%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
150% 30% Flow Reduction
40% Flow Reduction

100%

50%

0%
April

August
May

July
January

February

November
September

October

December
June
March

517
 Hydropsyche spp. (1940-1969)
Northern Withlacoochee River - Reach 7

160%

140%

120%

100%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
80%
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
518
 Hydropsyche spp. (1970-1999)
Withlacoochee River - Reach 7

250%

200%
Habitat Gain/Loss

150%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
100%

50%

0%
April

August
May

July
January

February

November
September

October

December
June
March

519
 Hydroptila sp. - 1940-1969
Northern Withlacoochee River - Reach 7
100%

80%

60%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
520
 Hydroptila sp. 1970-1999
Northern Withlacoochee River - Reach 7

160%

140%

120%

100%
Habitat Gain / Loss

10% Flow Reduction

80%
20% Flow Reduction
30% Flow Reduction
60%
40% Flow Reduction

40%

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
521
 Stenelmis sp. 1940-1969
Northern Withlacoochee River - Reach 7
140%

120%

100%
Habitat Gain / Loss

80%

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

December
522
 Stenelmis sp. 1970-1999
Northern Withlacoochee River - Reach 7

180%

160%

140%

120%
Habitat Gain / Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80%
40% Flow Reduction

60%

40%

20%

0%
April

August
May

July
February
January

November
March

June

October
September

523 December
 T otal C hironomidae 1940-1969
Northern Withlac ooc hee R iver - R eac h 7

60%

50%

40%

30%
Habitat G ain / L os s

20%
10% F low R eduction
10% 20% F low R eduction
30% F low R eduction
0% 40% F low R eduction

-10%

-20%

-30% A ug us t
A pril

November
Oc tober

Dec ember
S eptember
J une

J uly
J anuary

F ebruary

May
Marc h

524
 Total Chironomidae 1970-1999
Northern Withlacoochee River - Reach 7

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

525 December
 Cricotopus bicinctus 1940-1969
Northern Withlacoochee River - Reach 7

30%

25%

20%

15%
Habitat Gain / Loss

10%
10% Flow Reduction
20% Flow Reduction
5%
30% Flow Reduction
40% Flow Reduction
0%

-5%

-10%

-15%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
526
 Cricotopus bicinctus 1970-1999
Northern Withlacoochee River - Reach 7

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
527
 Tanytarsus sp. C 1940-1969
Northern Withlacoochee River - Reach 7

60%

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%
April

August
May

July
February
January

November
March

June

October
September

December
528
 Tanytarsus sp. C 1970-1999
Northern Withlacoochee River - Reach 7

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
April

August
May

July
February
January

November
March

June

October
September

529 December
 Parakiefferiella sp. 1940-1969
Northern Withlacoochee River - Reach 7

30%

20%

10%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
0%
30% Flow Reduction
40% Flow Reduction

-10%

-20%

-30%
April

August
May

July
February
January

November
March

June

October
September

December
530
 Parakiefferiella sp. 1970-1999
Northern Withlacoochee River - Reach 7

100%

80%

60%
Habitat Gain / Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
531
 Pseudochironomus sp. 1940-1969
Northern Withlacoochee River - Reach 7

40%

30%

20%

10%
Habitat Gain / Loss

0%
10% Flow Reduction
20% Flow Reduction
-10%
30% Flow Reduction
40% Flow Reduction
-20%

-30%

-40%

-50%

-60%
April

August
May

July
February
January

November
March

June

October
September

December
532
 Pseudochironomus sp. 1970-1999
Northern Withlacoochee River - Reach 7

80%

60%

40%
Habitat Gain / Loss

20% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-20%

-40%

-60%
April

August
May

July
February
January

November
March

June

October
September

533 December
 Stempellina sp. A 1940-1969
Northern Withlacoochee River - Reach 7

250%

200%
Habitat Gain / Loss

150%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
100%

50%

0%
April

August
May

July
February
January

November
March

June

October
September

December
534
 Stempellina sp. A 1970-1999
Northern Withlacoochee River - Reach 7

250%

200%
Habitat Gain / Loss

150%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
100%

50%

0%
April

August
May

July
February
January

November
March

June

October
September

535 December
 Tvetenia vitracies 1940-1969
Northern Withlacoochee River - Reach 7

120%

100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%

-40%
April

August
May

July
February
January

November
March

June

October
September

December
536
 Tvetenia vitracies 1970-1999
Northern Withlacoochee River - Reach 7

200%

150%
Habitat Gain / Loss

100%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
50%

0%

-50%
April

August
May

July
February
January

November
March

June

October
September

537 December
APPENDIX 6. Results of PHABSIM analysis (weighted usable areas) for targeted fish species.

538
 Largemouth bass
Northern Withlacoochee River - Reach 1
10000

9000

8000

7000
WUA (sq ft / 1000 linear ft)

6000

Adults
5000

Juveniles
4000

3000

2000

1000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

539
 WUA (sq ft / 1000 linear ft)

0
5000
10000
15000
20000
25000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

540
380

Discharge (cfs)
400
Suwannee bass

450

500

550

600
Northern Withlcoochee River - Reach 1

700

800

900

1000

1100

1200
Adult

Juvenile
 WUA (sq ft / 1000 linear ft)

0
5000
10000
15000
20000
25000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

541
380

Discharge (cfs)
Spotted sucker

400

450

500

550
Northern Withlacoochee River - Reach 1

600

700

800

900

1000

1100

1200
Adult

Juvenile
 WUA (sq ft / 1000 linear ft)

0
2000
4000
6000
8000
10000
12000
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400

542
450
500
550
American eel

600
700
800
900
Northern Withlacoochee River - Reach 1

1000
1100
1200
Adult
 Largemouth bass
Northern Withlacoochee River - Reach 2
4500

4000

3500

3000
WUA (sq ft / 1000 linear ft)

2500
Adults

2000 Juveniles

1500

1000

500

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

543
 Suwannee bass
Northern Withlcoochee River - Reach 2
16000

14000

12000
WUA (sq ft / 1000 linear ft)

10000

Adult
8000
Juvenile

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

544
 Spotted sucker
Northern Withlacoochee River - Reach 2
12000

10000

8000
WUA (sq ft / 1000 linear ft)

Adult
6000

Juvenile

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

545
 American eel
Northern Withlacoochee River - Reach 2
10000

9000

8000

7000
WUA (sq ft / 1000 linear ft)

6000

5000
Adult

4000

3000

2000

1000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
Discharge (cfs)

546
 Largemouth bass
Northern Withlacoochee River - Reach Run 2
16000

14000

12000
WUA (sq ft / 1000 linear ft)

10000

Adults
8000

Juveniles

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

547
 Suwannee bass
Northern Withlcoochee River - Reach Run 2
30000

25000

20000
WUA (sq ft / 1000 linear ft)

Adult
15000
Juvenile

10000

5000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

548
 Spotted sucker
Northern Withlacoochee River - Reach Run 2
50000

45000

40000

35000
WUA (sq ft / 1000 linear ft)

30000

Adult
25000

Juvenile
20000

15000

10000

5000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

549
 WUA (sq ft / 1000 linear ft)

0
1000
2000
3000
4000
5000
6000
135 7000

145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400

550
450
500
550
American eel

600
700
800
900
Northern Withlacoochee River - Reach Run 2

1000
1100
1200
Adult
 Largemouth bass
Northern Withlacoochee River - Reach 3
9000

8000

7000

6000
WUA (sq ft / 1000 linear ft)

5000
Adults

4000 Juveniles

3000

2000

1000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

551
 Suwannee bass
Northern Withlcoochee River - Reach 3
14000

12000

10000
WUA (sq ft / 1000 linear ft)

8000
Adult

Juvenile
6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

552
 Spotted sucker
Northern Withlacoochee River - Reach 3
35000

30000

25000
WUA (sq ft / 1000 linear ft)

20000
Adult

15000 Juvenile

10000

5000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

553
 WUA (sq ft / 1000 linear ft)

0
500
1000
1500
2000
2500
3000
135 3500

145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400

554
American eel

450
500
550
600
Northern Withlacoochee River - Reach 3

700
800
900
1000
1100
1200
Adult
 Largemouth bass
Northern Withlacoochee River - Reach 3.5
12000

10000

8000
WUA (sq ft / 1000 linear ft)

Adults
6000

Juveniles

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

555
 Suwannee bass
Northern Withlcoochee River - Reach 3.5
16000

14000

12000
WUA (sq ft / 1000 linear ft)

10000

Adult
8000
Juvenile

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

556
 Spotted sucker
Northern Withlacoochee River - Reach 3.5
35000

30000

25000
WUA (sq ft / 1000 linear ft)

20000
Adult

15000 Juvenile

10000

5000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

557
 WUA (sq ft / 1000 linear ft)

0
1000
2000
3000
4000
5000
6000
135 7000

145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400

558
450
American eel

500
550
600
700
800
Northern Withlacoochee River - Reach 3.5

900
1000
1100
1200
Adult
 Largemouth bass
Northern Withlacoochee River - Reach 4
8000

7000

6000
WUA (sq ft / 1000 linear ft)

5000

Adults
4000

Juveniles

3000

2000

1000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

559
 Suwannee bass
Northern Withlcoochee River - Reach 4
20000

18000

16000

14000
WUA (sq ft / 1000 linear ft)

12000
Adult
10000
Juvenile
8000

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

560
 WUA (sq ft / 1000 linear ft)

0
5000
10000
15000
20000
25000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

561
380

Discharge (cfs)
400
Spotted sucker

450

500

550
Northern Withlacoochee River - Reach 4

600

700

800

900

1000

1100

1200
Adult

Juvenile
 American eel
Northern Withlacoochee River - Reach 4
45000

40000

35000

30000
WUA (sq ft / 1000 linear ft)

25000

Adult
20000

15000

10000

5000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
Discharge (cfs)

562
 Largemouth bass
Northern Withlacoochee River - Reach 5
1000

900

800

700
WUA (sq ft / 1000 linear ft)

600

Adults
500

Juveniles
400

300

200

100

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

563
 WUA (sq ft / 1000 linear ft)

0
1000
2000
3000
4000
5000
6000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

564
380

Discharge (cfs)
400

450
Suwannee bass

500

550

600
Northern Withlcoochee River - Reach 5

700

800

900

1000

1100

1200
Adult

Juvenile
 WUA (sq ft / 1000 linear ft)

0
1000
2000
3000
4000
5000
6000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

565
Discharge (cfs)
400
Spotted sucker

450

500

550
Northern Withlacoochee River - Reach 5

600

700

800

900

1000

1100

1200
Adult

Juvenile
 WUA (sq ft / 1000 linear ft)

0
2000
4000
6000
8000
10000
12000
14000
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400
American eel

566
450
500
550
600
Northern Withlacoochee River - Reach 5

700
800
900
1000
1100
1200
Adult
 Largemouth bass
Northern Withlacoochee River - Reach 6
8000

7000

6000
WUA (sq ft / 1000 linear ft)

5000

Adults
4000

Juveniles

3000

2000

1000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

567
 WUA (sq ft / 1000 linear ft)

0
2000
4000
6000
8000
10000
12000
135

145

155

165

180

200

220

240

260

280

300

320

340

360

568
380

Discharge (cfs)
400
Suwannee bass

450

500

550
Northern Withlcoochee River - Reach 6

600

700

800

900

1000

1100

1200
Adult

Juvenile
 Spotted sucker
Northern Withlacoochee River - Reach 6
20000

18000

16000

14000
WUA (sq ft / 1000 linear ft)

12000

Adult
10000

Juvenile
8000

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

569
 American eel
Northern Withlacoochee River - Reach 6
16000

14000

12000
WUA (sq ft / 1000 linear ft)

10000

8000
Adult

6000

4000

2000

0
135
145
155
165
180
200
220
240
260
280
300
320
340
360
380
400
450
500
550
600
700
800
900
1000
1100
1200
Discharge (cfs)

570
 Largemouth bass
Northern Withlacoochee River - Reach Run 7
12000

10000

8000
WUA (sq ft / 1000 linear ft)

Adults
6000

Juveniles

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

571
 Suwannee bass
Northern Withlcoochee River - Reach Run 7
20000

18000

16000

14000
WUA (sq ft / 1000 linear ft)

12000
Adult
10000
Juvenile
8000

6000

4000

2000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

572
 Spotted sucker
Northern Withlacoochee River - Reach Run 7
35000

30000

25000
WUA (sq ft / 1000 linear ft)

20000
Adult

15000 Juvenile

10000

5000

0
135

145

155

165

180

200

220

240

260

280

300

320

340

360

380

400

450

500

550

600

700

800

900

1000

1100

1200
Discharge (cfs)

573
 WUA (sq ft / 1000 linear ft)

0
1000
2000
3000
4000
5000
6000
135 7000

145
155
165
180
200
220
240
260
280
300
320
340
360
380

Discharge (cfs)
400

574
American eel

450
500
550
600
700
Northern Withlacoochee River - Reach Run 7

800
900
1000
1100
1200
Adult
APPENDIX 7. Time series analyses of targeted fish species PHABSIM results.

575
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 1

350%

300%

250%
Habitat Gain / Loss

200%
10% Flow Reduction
20% Flow Reduction

150% 30% Flow Reduction

40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 1

350%

300%

250%
Habitat Gain / Loss

200%
10% Flow Reduction
20% Flow Reduction

150% 30% Flow Reduction

40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November

December
September

576
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 1

70%

60%

50%

40%
Habitat Gain / Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 1

70%

60%

50%

40%
Habitat Gain / Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20%
40% Flow Reduction

10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

577
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 1
140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction
60% 30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 1

140%

120%

100%
Habitat GAin / Loss

80%
10% Flow Reduction
20% Flow Reduction

60% 30% Flow Reduction

40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

578
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 1

40%

30%

20%
Habitat Gain / Loss

10% 10% Flow Reduction

20% Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 1

40%

30%

20%
Habitat Gain / Loss

10% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
0% 40% Flow Reduction

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December

579
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 1
200%

180%

160%

140%

120%
Habitat Gain/Loss

100% 10% Flow Reduction

20% Flow Reduction
80% 30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 1
200%

180%

160%

140%

120%
Habitat Gain/Loss

10% Flow Reduction

100% 20% Flow Reduction
30% Flow Reduction
80% 40% Flow Reduction

60%

40%

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

580
 Adult Spotted Sucker (1940-1969)
Withlacoochee River - Shoal 1

60%

50%

40%

30%
Habitat Gain/Loss

20%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%

-40%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 1

60%

50%

40%

30%
Habitat Gain/Loss

20%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
0%

-10%

-20%

-30%

-40%
August
July
April

May

June

October
March
January

February

November
September

December

581
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 1

100%

90%

80%

70%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Withlacoochee River - Shoal 1

100%

90%

80%

70%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

582
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 2

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 2

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

583
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 2

60%

50%

40%
Habitat Gain / Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 2

60%

50%

40%
Habitat Gain / Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

584
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 2
80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction

30% 40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 2

80%

70%

60%

50%
Habitat GAin / Loss

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

585
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 2

60%

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 2

60%

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

586
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 2
90%

80%

70%

60%
Habitat Gain/Loss

50% 10% Flow Reduction

20% Flow Reduction

40% 30% Flow Reduction

40% Flow Reduction

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 2
90%

80%

70%

60%
Habitat Gain/Loss

10% Flow Reduction

50% 20% Flow Reduction
30% Flow Reduction

40% 40% Flow Reduction

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

587
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 2

60%

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 2

60%

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction
10% 40% Flow Reduction

0%

-10%

-20%

-30%
August
July
April

May

June

October
March
January

February

November
September

December

588
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 2

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 2

100%

80%

60%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

589
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Run 2

70%

60%

50%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Run 2

70%

60%

50%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

590
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Run 2

50%

40%
Habitat Gain / Loss

30%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Run 2

50%

40%
Habitat Gain / Loss

30%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

591
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Run 2
60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Run 2

60%

50%

40%
Habitat GAin / Loss

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

592
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Run 2

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Run 2

50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

593
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Run 2

140%

120%

100%
Habitat Gain/Loss

80% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
60% 40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Run 2
140%

120%

100%
Habitat Gain/Loss

10% Flow Reduction

80%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December

594
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Run 2

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Run 2

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

595
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Run 2

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Run 2

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

596
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 3

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 3

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

30% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

597
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 3

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 3

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

598
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 3
50%

40%

30%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 3

50%

40%

30%
Habitat GAin / Loss

10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

599
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 3

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
30% Flow Reduction
40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 3

30%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
40% Flow Reduction

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

600
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 3
80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 3
80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

601
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 3

60%

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 3

60%

50%

40%

30%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
20%
30% Flow Reduction
40% Flow Reduction
10%

0%

-10%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

602
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 3

25%

20%

15%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction

5%

0%

-5%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 3

25%

20%

15%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction

5%

0%

-5%
August
July
April

May

June

October
March
January

February

November
September

December

603
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 3.5

70%

60%

50%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 3.5

70%

60%

50%
Habitat Gain / Loss

40%
10% Flow Reduction
20% Flow Reduction

30% 30% Flow Reduction

40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

604
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 3.5

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 3.5

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

605
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 3.5
40%

35%

30%

25%
Habitat Gain / Loss

10% Flow Reduction

20%
20% Flow Reduction
30% Flow Reduction

15% 40% Flow Reduction

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 3.5

40%

35%

30%

25%
Habitat GAin / Loss

10% Flow Reduction

20% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
15%

10%

5%

0%
August
July
April

May

June

October
March
January

February

November

December
September

606
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 3.5

40%

35%

30%

25%
Habitat Gain / Loss

20%
10% Flow Reduction
20% Reduction
15%
30% Flow Reduction
40% Flow Reduction
10%

5%

0%

-5%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 3.5

40%

35%

30%

25%
Habitat Gain / Loss

20%
10% Flow Reduction
20% Flow Reduction
15%
30% Flow Reduction
40% Flow Reduction
10%

5%

0%

-5%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

607
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 3.5
300%

250%

200%
Habitat Gain/Loss

10% Flow Reduction

150% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 3.5
300%

250%

200%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
150% 30% Flow Reduction
40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November
September

December

608
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 3.5

60%

50%

40%
Habitat Gain/Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 3.5

60%

50%

40%
Habitat Gain/Loss

30% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

609
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 3.5

35%

30%

25%

20%
Habitat Gain/Loss

15%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%

-15%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 3.5

35%

30%

25%

20%
Habitat Gain/Loss

15%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%

-15%
August
July
April

May

June

October
March
January

February

November
September

December

610
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 4

450%

400%

350%

300%
Habitat Gain / Loss

250%
10% Flow Reduction
20% Flow Reduction
200% 30% Flow Reduction
40% Flow Reduction
150%

100%

50%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 4

450%

400%

350%

300%
Habitat Gain / Loss

250%
10% Flow Reduction
20% Flow Reduction
200% 30% Flow Reduction
40% Flow Reduction
150%

100%

50%

0%
August
July
April

May

June

October
March
January

February

November

December
September

611
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 4

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 4

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

612
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 4
350%

300%

250%
Habitat Gain / Loss

200%
10% Flow Reduction
20% Flow Reduction
150% 30% Flow Reduction
40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 4

350%

300%

250%
Habitat GAin / Loss

200%
10% Flow Reduction
20% Flow Reduction

150% 30% Flow Reduction

40% Flow Reduction

100%

50%

0%
August
July
April

May

June

October
March
January

February

November

December
September

613
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 4

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
30%
30% Flow Reduction
40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 4

60%

50%

40%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
30%
30% Flow Reduction
40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

614
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 4
120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 4
120%

100%

80%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
60% 30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December

615
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 4

45%

40%

35%

30%
Habitat Gain/Loss

10% Flow Reduction

25%
20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

15%

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 4

45%

40%

35%

30%
Habitat Gain/Loss

10% Flow Reduction

25%
20% Flow Reduction
30% Flow Reduction
20% 40% Flow Reduction

15%

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December

616
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 4

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 4

80%

70%

60%

50%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

617
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 5

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

60% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

618
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 5

110%

100%

90%

80%

70%
Habitat Gain / Loss

10% Flow Reduction

60%
20% Flow Reduction

50% 30% Flow Reduction

40% Flow Reduction

40%

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 5

110%

100%

90%

80%

70%
Habitat Gain / Loss

10% Flow Reduction

60%
20% Flow Reduction

50% 30% Flow Reduction

40% Flow Reduction

40%

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

619
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 5
90%

80%

70%

60%
Habitat Gain / Loss

50%
10% Flow Reduction
20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 5

90%

80%

70%

60%
Habitat GAin / Loss

50%
10% Flow Reduction
20% Flow Reduction
40% 30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November

December
September

620
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 5

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 5

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

621
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 5

300%

260%

220%
Habitat Gain/Loss

180% 10% Flow Reduction

20% Flow Reduction
140% 30% Flow Reduction
40% Flow Reduction

100%

60%

20%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 5

300%

260%

220%
Habitat Gain/Loss

180% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
140%
40% Flow Reduction

100%

60%

20%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

622
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 5

70%

60%

50%
Habitat Gain/Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 5

70%

60%

50%
Habitat Gain/Loss

40% 10% Flow Reduction

20% Flow Reduction
30% Flow Reduction
30% 40% Flow Reduction

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

623
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 5

180%

160%

140%

120%
Habitat Gain/Loss

100%
10% Flow Reduction
20% Flow Reduction
80%
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%

-20%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 5

180%

160%

140%

120%
Habitat Gain/Loss

100%
10% Flow Reduction
20% Flow Reduction
80%
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December

624
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 6

140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction

60% 30% Flow Reduction

40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Shoal 6

140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction

60% 30% Flow Reduction

40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

625
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Shoal 6

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Shoal 6

80%

70%

60%

50%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
40%
30% Flow Reduction
40% Flow Reduction
30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

626
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 6
30%

25%

20%

15%
Habitat Gain / Loss

10% Flow Reduction

10%
20% Flow Reduction
30% Flow Reduction

5% 40% Flow Reduction

0%

-5%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Shoal 6

30%

25%

20%

15%
Habitat GAin / Loss

10% Flow Reduction

10% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
5%

0%

-5%

-10%
August
July
April

May

June

October
March
January

February

November

December
September

627
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Shoal 6

30%

25%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Reduction
15%
30% Flow Reduction
40% Flow Reduction

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Shoal 6

30%

25%

20%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
15%
30% Flow Reduction
40% Flow Reduction

10%

5%

0%
August
July
April

May

June

October
March
January

February

November
September

December

628
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 6
140%

120%

100%
Habitat Gain/Loss

80%
10% Flow Reduction
20% Flow Reduction
30% Flow Reduction
60%
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 6
140%

120%

100%
Habitat Gain/Loss

10% Flow Reduction

80%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
60%

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December

629
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Shoal 6

35%

30%

25%

20%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
15%
30% Flow Reduction
40% Flow Reduction
10%

5%

0%

-5%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Shoal 6

35%

30%

25%

20%
Habitat Gain/Loss

10% Flow Reduction

20% Flow Reduction
15%
30% Flow Reduction
40% Flow Reduction
10%

5%

0%

-5%
August
July
April

May

June

October
March
January

February

November
September

December

630
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Shoal 6

20%

18%

16%

14%
Habitat Gain/Loss

12%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
8%

6%

4%

2%

0%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Shoal 6

20%

18%

16%

14%
Habitat Gain/Loss

12%
10% Flow Reduction
20% Flow Reduction
10%
30% Flow Reduction
40% Flow Reduction
8%

6%

4%

2%

0%
August
July
April

May

June

October
March
January

February

November
September

December

631
 Juvnile Largemouth Bass (1940-1969)
Northern Withlacoochee River - Run 7

140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction

60% 30% Flow Reduction

40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

Juvenile Largemouth Bass (1970-1999)

Northern Withlacoochee River - Run 7

140%

120%

100%
Habitat Gain / Loss

80%
10% Flow Reduction
20% Flow Reduction

60% 30% Flow Reduction

40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November

December
September

632
 Adult Largemouth Bass (1940-1969)
Northern Withlacoochee River - Run 7

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Largemouth Bass (1970-1999)
Northern Withlacoochee River - Run 7

120%

100%

80%
Habitat Gain / Loss

10% Flow Reduction

20% Flow Reduction
60%
30% Flow Reduction
40% Flow Reduction

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December

633
 Juvenile Suwannee Bass (1940-1969)
Northern Withlacoochee River - Run 7
100%

80%

60%
Habitat Gain / Loss

10% Flow Reduction

40%
20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Suwannee Bass 1970-1999
Northern Withlacoochee River - Run 7

100%

80%

60%
Habitat GAin / Loss

10% Flow Reduction

40% 20% Flow Reduction
30% Flow Reduction
40% Flow Reduction
20%

0%

-20%
August
July
April

May

June

October
March
January

February

November

December
September

634
 Adult Suwannee Bass (1940-1969)
Northern Withlacoochee River - Run 7

100%

90%

80%

70%

60%
Habitat Gain / Loss

10% Flow Reduction

50%
20% Reduction

40% 30% Flow Reduction

40% Flow Reduction

30%

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Suwannee Bass (1970-1999)
Northern Withlacoochee River - Run 7

100%

90%

80%

70%

60%
Habitat Gain / Loss

10% Flow Reduction

50%
20% Flow Reduction

40% 30% Flow Reduction

40% Flow Reduction
30%

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

635
 Juvenile Spotted Sucker (1940-1969)
Northern Withlacoochee River - Run 7

180%

160%

140%

120%
Habitat Gain/Loss

10% Flow Reduction

100%
20% Flow Reduction
30% Flow Reduction
80% 40% Flow Reduction

60%

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December
Juvenile Spotted Sucker (1970-1999)
Northern Withlacoochee River - Run 7
180%

160%

140%

120%
Habitat Gain/Loss

10% Flow Reduction

100% 20% Flow Reduction
30% Flow Reduction

80% 40% Flow Reduction

60%

40%

20%

0%
August
July
April

May

June

October
March
January

February

November
September

December

636
 Adult Spotted Sucker (1940-1969)
Northern Withlacoochee River - Run 7

110%

100%

90%

80%

70%
Habitat Gain/Loss

60% 10% Flow Reduction

20% Flow Reduction
50%
30% Flow Reduction

40% 40% Flow Reduction

30%

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December
Adult Spotted Sucker (1970-1999)
Northern Withlacoochee River - Run 7

110%

100%

90%

80%

70%
Habitat Gain/Loss

60% 10% Flow Reduction

20% Flow Reduction
50%
30% Flow Reduction

40% 40% Flow Reduction

30%

20%

10%

0%

-10%
August
July
April

May

June

October
March
January

February

November
September

December

637
 Adult American Eel (1940-1969)
Northern Withlacoochee River - Run 7

100%

90%

80%

70%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
August
July

October
April

May

June
Janua ry

November
March
February

December
September

Adult American Eel (1970-1999)

Northern Withlacoochee River - Run 7

100%

90%

80%

70%
Habitat Gain/Loss

60%
10% Flow Reduction
20% Flow Reduction
50%
30% Flow Reduction
40% Flow Reduction
40%

30%

20%

10%

0%
August
July
April

May

June

October
March
January

February

November
September

December

638