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Biotechnology is part of the Genetics unit of the IB Biology curriculum, be it SL or HL. The understandings cover the basics of what is required knowledge.
Biotechnology is part of the Genetics unit of the IB Biology curriculum, be it SL or HL. The understandings cover the basics of what is required knowledge.
Biotechnology is part of the Genetics unit of the IB Biology curriculum, be it SL or HL. The understandings cover the basics of what is required knowledge.
Biotechnology involves manipulating existing genetic systems in order to achieve a desired outcome.
http://www.dnai.org/
3.5 Genetic Modification and Biotechnology
things to remember from class 1:
- PCR, or amplifying DNA, is required for all other DNA technologies
Notes from Bioninja
Understandings
- PCR can be used to amplify small amounts of DNA
o PCR stands for polymerase chain reaction o Its an artificial method of replicating DNA under laboratory conditions It amplifies a small sample of a specific sequence of DNA into large quantities Each reaction cycle DOUBLES the amount of DNA A standard PCR sequence of 30 cycles creates over 1 billion copies, or 2^30 copies. o Stages of PCR Denaturation DNA sample is heated to separate it into two single strands This is at 95 degrees Celsius for 1 minute Annealing DNA primers attach to the 3 ends of the target sequence This is at 55 degrees Celsius for 1 minute Elongation A DNA polymerase that can stand heat (its called Taq) binds to the primer and copies the strand This is at 72 degrees Celsius for 2 minutes o Then the large quantities of DNA are isolated and manipulated using different techniques. - Gel electrophoresis is used to separate proteins or fragments of DNA according to size o This is the isolation part o So the DNA samples are put into a block of gel and then theyre electrocuted (an electric current goes through them) o That makes the samples move through the gel, right? o Right. And then smaller samples move quicker through the gel, cause they can o So what this does is it makes the samples separate, cause theyre moving at different speeds o Oh and it goes from the negative terminus, or the cathode, to the positive terminus, or the anode. o But DNA and protein separation follow different protocols DNA separation So the DNA could be cut into fragments with something called restriction endonuclease, and obviously different DNA samples make different fragment lengths cause theyre all different sizes So once these fragments are made they separate because DNA is negatively charged, right? o Right. Cause DNA has a PO4 3- on each nucleotide, and that phosphate group is negative. Okay, so then DNA samples are put into a gel called agarose gel Then this funky thing called a complementary radiolabelled hybridization probe, identifies a specific sequence Then it transfers it into a membrane - DNA profiling involves comparison of DNA - Genetic modification is carried out by gene transfer between species - Clones are groups of genetically identical organisms derived from a single original parent cell - Many plant species and some animal species have natural methods of cloning - Animals can be cloned at the embryo stage by breaking up the embryo into more than one group of cells - Methods have been developed for cloning adult animals using differentiated cells