EDT 03

1. The metabolism of lactose in cells requires 13. Following figure represent RNA. Find correct
three enzymes - statement about this type of RNA-
(A)permease, transferase, beta-galactosidase (A) It is also known as
(B)transacetylase, permease, alpha-galactosidase soluble RNA
(C)transcriptase, permease, beta-galactosidase (B) It can also be
(D)transacetylase, permease, beta-galactosidase explained by clover
2.Francois Jacob & J. Monod, proposed the leaf model
classical model of Lac operon which can explain (C) It carries message
properly gene expression & regulation in ______ for protein synthesis
(A)Escherichia coli (B) Bacillus subtilis from DNA to ribosomes
(C)Neurosporaspp (D) Proteus vulgaris (D) It remains associated with ribosomes
3.Ribosomes are composed of approximately permanently
________ protein & _______ r-RNA. 14. A segment of DNA that participates in
(A) 65% & 35% (B) 50% & 50% recombination through crossing over during meiosis
(C) 35% & 65 % (D) 80% & 20% is called as -
4. During formation of polypeptide chain, before (A) Cistron (B) Muton
process of elongation _________ take place. (C) Recon (D) none of these
(A)initiation (B) termination
(C)amino acids (D) translocation
5.The structural gene which is to be transcribed is 15. The process shown in the following figure is -
generally _________ in prokaryotic organisms.
(A)monocistronic (B)polycistronic
(C)acistronic (D)dicistronic
6. Central dogma of protein synthesis involves _
(A)transportation& translation
(B)transcription& translocation
(C)capping& tailing
(D)transcription& translation
7.The removal of introns from RNA is called _
(A)capping (B) tailing
(C)elimination (D) splicing (A) Translation (B) Transduction
8.After transcription in eukaryotes, mRNAs are- (C) Transcription (D) Transformation
(A)used in the same compartment 16. The successive nucleotides of DNA are
(B)degenerate covalently linked through
(C)transported out of nucleus (A) hydrogen bonds (B) phosphodiester bonds
(D)converted to t-RNAs (C) glycosidic bonds (D) ionic bond
9. Genetic code is ________ &comma less. 17. In eukaryotes DNA replication takes place ___
(A)Duplet (B) sometimes triplet (A) in endoplasmic reticulum
(C)transferable (D) always triplet (B) in cytoplasm
10.___________ is one of the important feature of (C)inside nucleus
the genetic code. (D) all of these
(A)non-polar nature (B) polarity 18. Unwinding of DNA strands is done by which
(C)great width (D) wobbling enzyme ?
11. ___________ proposed Wobble Hypothesis (A)helicase
(A) Bateson (B) Hugo Victor (B) amylase
(C) Crick (D) H.G. Khorana (C) ligase
12.Hair Pin model of t-RNA was proposed by- (D) endonuclease
(A) Hoagland (B) Copeland 19. Which of the following is not a Property of the
(C) Landsteiner (D) Holley genetic code?
(A) Non-overlapping (C) Degeneracy
(C) Universal (D) Ambiguous
20. Initiation codon is
(A) UAG (B) UUU
(C) UGA (D) AUG
21. A single DNA has how many base pairs in one (C) GUG (D) GGU
complete turn ? 32. Nucleoside is formed when the nitrogenous
(A) 11 (B) 72 (C) 10 (D) 9 bases are linked to
22. What is the correct sequence of processes (A)sugar (B) phosphate
involved in central dogma? (C) proteins (D) fats
(A) Replication translation, transcription 33. X-ray crystallographicdata of DNA was provided
(B) Translation, replication, transcription by
(C) Transcription, replication, translation (A) Watson and Crick
(D) Replication, transcription, translation (B) Wilkins and Rosalind Franklin
23. DNA consists of two complementary nucleotide (C) Betason and Punnett
chains. If sequence of nucleotides in one of the (D) Both (a) and (b)
chain is AGCTTCGA, then nucleotide sequence in 34. The thin & long nucleosome fiber is coiled like
the other chain shall be a telephone wire to make _______ fiber of 30 nm.
(A) TAGCATAT (C) GATCCTAG (A) Solenoid (B) Octamer
(C) GCTAAGCT (D) TCGAAGCT (C) H 1 (D) Euchromatin
24. Avery et.al. proved that 35. A base pair consists of a purine and pyrimidine
(A) DNA is made up of nucleotides. because
(B) DNA is the transforming principle. (A) the space available between two chains of
(C) DNA replicates by semi-conservative method DNA, i.e. 2 nm can accommodate only such pair
(D) transduction of DNA takes place withthe help (B) there is perfect match between hydrogen
of viruses. donor and acceptor sites on two bases
25. DNA was proved to be a genetic material in (C) two polynucleotide chains of DNA are
E.coli and bacteriophage in complementary to each other
(A) Miescher's experiment (D) All of the above
(B) Meselson-Stahl's experiment 36. phenomenon where the third base of t-RNA at
(C) Griffith's experiment its 5'end can pair with a non-complementary base
(D) Hershey-Chase's experiment of mRNA is called
26. Radioactive element used by Hershey andChase (A) universality (B) non-matching
to make the DNA core ofbacteriophages (C)wobbling (D) degeneracy
radioactive is 37. In sugar molecule of nucleotide, at 1-C
(A) 36P (B) 32S (C)32P (D) 36S position which of the following is attached?
(A) Phosphate group (B) Nitrogen base
27. Strongest evidence that DNA is a (C) Hydrogen molecule (D) Methyl group
geneticmaterial comes from 38. Identify the pair of nucleotides which are
(A) the fact that chromosomes contain DNA joined by 3 hydrogen bonds in double stranded
(B)transformation experiment of bacterial cell DNA
(C) the fact that DNA is present in nucleus (A) AG (B) AT (C) TG (D) CG.
(D) the fact that DNA is not present in nucleus 39. During DNA replication, the term leading
28. Gene is a strand is applied to the one which replicates in
(A) unit of inheritance (A) 5' 3' direction discontinuously
(B) segment of DNA which Providesinstructions for (B) 5' 3' direction continuously
synthesis of specificprotein (C) 3' 5' direction continuously
(C) segment of DNA responsible forexpression of a (D) 3' 5' direction discontinuously.
particular character
(D) all of the above
29. The length of DNA usually depends on
(A)number of nucleotides 40. The messenger RNA is formed
(B) position of nucleotides (A) in cytoplasm
(C) Type of nucleotides (B) in endoplasmic reticulum
(D) Both A & B (C)from DNA in nucleus
30. Name the pyrimidine which is present only in (D) by free ribosomes
RNA 41. Entry of lactose in cell is influenced by _____
(A) Adenine (B) Uracil (A) hydrogenase (B) ligase
(C) Guanine (D) Thymine (C) oxidase (D) permease
31. Which of the following is stop codon ?
(A) UAA (B) AUG
42. Enzyme aminoacyl t-RNA synthetase is (C) Operon - Structural genes, operator and
responsible for promoter.
(A) initiating m-RNA synthesis (D) Transcription - Writing information from DNA
(B) combining t-RNA at P site of m-RNA to r-RNA
(C)charging t-RNA molecules
(D) nothing
43. Meselson and Stahl experiment proved 54. Lac operon in E. coli, is induced by
(A) DNA is a genetic material (A) glucose (B) promoter gene
(B) central dogma (C) Beta-galactosidase (D) lactose.
(C) transformation 55. Out of 64 codons, 61 codons code for 20 types
(D)semi-conservative DNA replication of amino acid. It is called
44. In which of the following stage, does synthesis (A) overlapping of gene
of polypeptide takes place ? (B) wobbling of codon
(A) Transcription (B) Initiation (C)degeneracy of genetic code
(C) Translation (D) Replication (D) universality of codons.
45. On which strand of DNA, m-RNA is 56. Hargobind Khorana was awarded the Nobel
constructed ? Prize for-
(A) Non-sense strand (B) Sense strand (A)genetic code (B) disease resistant maize
(C) Both the strands (D) Anti-sense strand (C) gene synthesis (D) discovery of transposons.
46. A beads on a string' like chromatin with 200 57. The sugar in nucleic acids is _________.
beads or nucleosomes contains ..... bp of DNA (A) hexose (B) pentose
(A)80000 (B)40000 (C) sedoheptulose (D) arabinose
(C)20000 (D) 10000 58. How many monocyclic type of bases are
47. The packaging of chromatin at higher level found in a nucleic acid?
requires an additional set of proteins that are (A) 2 (B) 3 (c) 4 (D) 6
collectivelyreferred to as ___________ 59. The 3' - 5' phosphodiester linkages inside a
(A) histone proteins (B) non-histone proteins polynucleotide chain serve to join
(C) basic proteins (D) acidic packaging proteins (A) one DNA strand with the other DNA strand
48. The RNA act as genetic material in some _____ (B) one nucleoside with another nucleoside
(A) Bacteria (B) Viruses (C)one nucleotide with another nucleotide
(C) yeasts (D) Algae (D) one nitrogenous base with pentose sugar.
49. H-1, histone is also called 60. The ernpirical formula of ribose sugar is
(A) junk histone (B) Linker histone (A) C5H10O4 (B) C5H10O5
(C) neutral histone (D) basic histone (C) C5H9O4 (D) C4H10O4
50. In genetic coding, triplet of nitrogen bases 61. Operon concept was given by
make possible 64 types of codons. A doublet codon (A) Jacob & Monod (B) Crick
system would have made possible (C) Chase (D) Rosalind Franklin
(A) 64 types of codons (B) 4 types of codons 62. The joining of amino acids to form a
(C) 24 types of codons (D) 16 types of codons polypeptide occurs during
51. What will be the number of histone molecules (A)translation (B) transcription
in a chromatin fiber having 50 nucleosomes? (C) replication (D) reverse transcription.
(A) 400 (B) 450 63. In 1869, Friedrich Miescher separated a
(C) 500 (D) 1000 cellular substance from the nuclei of pus cells
52. The sequence of nucleotides AUGCUUCUC called
indicates that it is a segment of (A) deoxyribonuclease (B) peptide
(A) sense strand of DNA (C)nuclein (D) ribose
(B) anti sense strand of DNA 64. Okazaki fragments are synthesized on
(C) RNA (A) lagging strands of RNA only
(D) polypeptide chain. (B)lagging strands of DNA only
53. Which one of the following is wrongly (C) both leading and lagging strands of DNA
matched? (D) supplementary DNA
(A) Translation - Using information in m-RNA to 65. Genetic material should be
make protein. (A) chemically stable
(B) Repressor protein - Binds to operator to stop (B) able to express accurately
enzyme synthesis. (C) able to replicate with minimum errors
(D) all of the above
66. Who introduced the term Cistron, Muton and 77. DNA is a
Recon? (A) long polymer of deoxyribonucleotides
(A) Avery and Macleod (B) Hershey and Chase (B) short polymer of ribonucleotides
(C) Seymour Benzer (D) Griffith (C) monomer of deoxyribonucleotides
67. S-type strain of Diplococcus pneumoniae is (D) long polymer of ribonucleotides
(A) non-capsulated, avirulent, rough 78. Which is not related with eukaryotic mRNA?
(B) capsulated, avirulent, rough (A) Intron, exon (B) Ribosomes binding site
(C) non-capsulated, virulent, smooth (C) Poly A tail (D) Okazaki piece
(D)capsulated, virulent, smooth 79. The distance between two consecutive
68. The number of triplet codons having all the nucleotide pairs in DNA is
three bases same in 64 triplet codons is (A) 34 A0 (B) 3.4 A0
0
(A) 2 (B) 4 (C) 6 (D) 8. (C) 0.34 A (D) 20 A0
69. If we inject a mice with the mixture of 80. Genetic code is
heatkilled S-type and living R-type, then (A) sequence of nitrogenous bases on mRNA
(A) the mice will survive (B) sequence of nitrogenous bases on tRNA
(B)the mice will develop pneumonia and die (C) sequence of nitrogenous bases on rRNA
(C) the metabolic rate of the mice will increase (D)sequence of nitrogenous bases on DNA.
(D) none of these 81. Statement 1:- One codon may code for more
70. The capsule surrounding the S-type of than one amino acid.
bacteriais a Statement 2 :- A codon is degenerate and
(A) lipid coat (B) polysaccharidecoat ambiguous.
(C) protein coat (D) monosaccharidecoat (A) both the statements are true
71. Before the discovery of DNA, the proteins were (B) statement 1 is correct & statement 2 is false
considered as genetic material because (C) statement 2 is correct & statement 1 is false.
(A) most of the enzymes are proteins (D)both assertion and reason are false.
(B) proteins are formed from amino acids 82. The initiating codon in eukaryotes is
(C) proteins seemed to be much more diverse (A)methionine (B) valine
(D) None of the above (C) f-met (D) all of these
72. Griffith's experiment proved that 83. The structural gene which is to be transcribed
(A) DNA is the genetic material is generally _________ in eukaryotes.
(B) there are two strains of Streptococcus (A)polycistronic (B) tricistronic
pneumoniae (C) dicistronic (D) monocistronic
(C) some transforming factor transformed rough 84. Non-pathogenic and avirulent strain of
bacteria into smooth bacteria Diplococcus pneumoniae is/are
(D) RNA is the transforming factor (A) S-type (B) SR type
73. Hershey and Chase used ... and ... to label DNA (C) R-type (D) Both (A) and (C)
and protein respectively. 85. Which of the following is true for DNA helicase
(A)32P and 35S (B) 121I and 235U (A) it is DNA unwinding protein
35 32
(C) S and P (D) 37P and 41S (B) Promote separation of the two parental strands
74. Nuclein is and establish replication forks.
(A)acidic (B) basic (C) also called as rep protein
(C) neutral (D) none of these (D) All of the above
75. Who proved that DNA is the genetic material & 86. The sequence of structural gene in lac operon
not protein. concept is
(A) Griffith (A) lac a, lac y, lac z (B) lac a, lac z, lac y
(B) Avery, Macleod and McCarty (C) lac y, lac z, lac a (D) lac z, lac y, lac a
(C) Alfred Hershey and Martha Chase 87. The function of t-RNA is to
(D) Mendel and Watson (A) form a site for DNA synthesis
76. Experimental organism of Griffith was (B) transport the genetic code.
(A) Variola virus (B) Tuberculosis bacteria (C)transport specific amino acids to specific sites
(C) Actinomycetes (D) Diplococcus pneumoniae on the m-RNA.
(D) synthesize amino acids.
88. t-RNA binds to ribosome by its
(A) anticodon loop (B) T C loop
(C) DHU loop (D) AA - site
89. Genetic code means the _________
(A) relationship in the sequence of bases in DNA /
RNA
(B) relationship in the sequence of amino acids in
polypeptides
(C)relationship between the sequence of bases in
DNA/RNA and the sequence of amino acids in
polypeptide
(D) none of these
90. The two triplet codons UUU and UUC specify
the same amino acid, i.e. phenylalanine. This
property of genetic code is called
(A)degeneracy of code (B) rusting of code
(C) commaless code (D) non-ambiguity code
91. Degeneracy of genetic code is compensated by
(A) polarity of codon
(B) Wobble hypothesis
(C) Okazaki fragments
(D) non-overlapping codons
92. During DNA replication
(A)the lagging strand produces Okazaki fragments
(B) the hydrogen bonds between the nucleotides of
same strands break
(C) the phosphodiester bonds between the
nucleotides break
(D) the bonds between the nitrogen base and
deoxyribose sugar break
93. DNA template refers to
(A) the physical basis of heredity
(B)unit of DNA having coded information and acts
as a model from which information is collected by
m-RNA
(C) a genetic element which may or may not be
present in a cell associated with the chromosomes
(D) a complete set of chromosomes which is
transferred to the gametes
94. Enzyme necessary for transcription is
(A) DNA polymerase (B) RNA polymerase
(C) endonuclease (D) RNA ase
95. The strand of DNA which is transcribed is
called
(A) sense strand (B) coding strand
(C)anti-sense strand(D) supplementary strand
96. Regulation of lac operon by the repressor is an
example of ________ control of transcription
initiation.
(A) positive (B) negative
(C) neutral (D) best
97. If double-stranded DNA with 15N undergoes
replication twice in a normal medium, then which
of
the following is correct?
(A) Half bacteria contain 15N in DNA
(B) All four contains 15N in DNA
(C) None contains 15N in DNA
(D) 3/4 bacteria 15N in DNA
98. Continuous and discontinuous strands during
DNA replication are respectively called
(A)leading strand and lagging strand
(B) lagging strand and leading strand
(C) trailing strand and leading strand
(D) leading strand and lacking strand
99. Which of the following is a major difference in
replication in prokaryotes and eukaryotes?
(A) Replication in prokaryotes is conservative while
it is semiconservative in eukaryotes
(B) Prokaryotes do not require small RNA primer
for initiation of replication as in eukaryotes
(C) Prokaryotes have single origin of-replication
and eukaryotes have multiple origin of replication
(D) All of the above
100. How many base pairs of DNA helix are
contained in a typical nucleosome?
(A) 400 (B) 300 (C) 2OO (D) 100