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(1 Mark)
1. What does H ind and III refer to in the enzyme Hind III?
2. Some enzymes are considered as molecular scissors. in genetic engenrring. What is the name assigned to such enzymes?
3. Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
5. Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
(2, 3Marks)
(b) Bioreactors
7. . Explain briefly
(a) PCR
(c) Chitinase
8. Distinguish between
9. What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
10. A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment
11. What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which
(5Marks)
12. For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a
14. A selectable marker is used in the section of recombinants on the basis of their ability to produce colour in presence of
chromogenic substrate.
15. Describe the process of DNA Recombination in detail with help of diagram.
By-K C MEENA PGT BIOLOGY
KV VIKASPURI II SHIFT