Transcription and Translation Simulation Worksheet

-Go to the website: http://www.zerobio.com/drag_oa/protein/transcription.htm to fill out this

worksheet.

Step 1: Read the information about transcription. Drag the letters and drop them into the
appropriate box.

What is the mRNA sequence? _____________________________________________

Step 2: Record your answers to questions 1-5 here. Check your answers.

1. Which base in RNA is replaced by uracil?

2. How man mRNA codons are illustrated above?

3. What is the name of the enzyme that creates the mRNA copy from the DNA?

4. What is the name of the sugar in the mRNA nucleotides?

5. What is the mRNA transcript for the DNA sequence, TTACGC

Step 3: Click next at the bottom right of the page. Read the information about
translation. Drag the bases to their appropriate position complementary to the DNA.

What is the mRNA sequence?______________________________________________

Using the chart at the end of this document, what are the correct amino acids?
_________________________

Step 3: Record your answers to questions 1-4 here. Check your answers.

1. What organelle assists tRNA in translating the mRNA in the cytoplasm?

2. The role of tRNA is to carry a(n):

3. Is a tRNA anticodon more similar to DNA or RNA in nucleotide sequence?

4. If the mRNA codon is CGA, the tRNA anticodon that binds with it is:
Step 4: Click next at the bottom right. Protein Synthesis 1. Put it all together. Fill in the
table below.

Step 5: Record your answers to questions 1-4 here. Check your answers.

1. How many amino acids are coded for by the DNA?

2. What protein does this DNA code for hemoglobin or insulin? Hemoglobin: VAL-HIS-LEU
Insulin: GLY-ILE-VAL

3. If instead of ACT, the first DNA triplet was ACG, which amino acid would be coded for?

4. What amino acid is carried by a tRNA with the anticodon, GUA?

Step 6: Click next in the bottom right. Protein Synthesis 2. Fill in the chart below.

Step 7: Record your answers to questions 1-5 here. Check your answers.

1. Other than UUU, what is another mRNA codon that codes for Phenylalanine (PHE)?

2. What is the tRNA anticodon that binds to the codon you typed above?

3. The role of the ribosome is to help mRNA and tRNA interact. It is made of two subunits and is
roughly half____ and protein.

4. Sickle cell anemia is a disease of red blood cells in which a genetic mutation in DNA leads to
a mutation in hemoglobin. A single base change alters the DNA sequence CTC to CAC which
codes for the wrong amino acid. What amino acid is coded for by the normal DNA sequence,
CTC?

5. For the question above, what amino acid is coded for by the mutated DNA sequence, CAC?