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Laboratory Report BIO 2073 Bioprocess Plant & Equipment

Experiment 1
Exposure to bioreactor components

Objective
1. Identify the bioreactor component correctly
2. Explain the function of each component correctly

STIRRED TANK BIOREACTOR

Example of stirred tank bioreactor diagram

Experiment 1 Exposure to Bioreactor -1-


Laboratory Report BIO 2073 Bioprocess Plant & Equipment

PART AND IDENTIFY COMPONENT

Part Identify
An impeller is rotor inside a tube or conduit to increase
Impeller
the pressure and flow of fluid.

Is an electrode where the unit of measurement


pH electrode
describing the acidity or alkalinity of a mixture.

Level sensor is part which display liquid levels in


Level sensor
bioreactor.

Air inlet is the electrode to pass thought the air to the


Air inlet
bioreactor.

Ring sparger is component part in the bioreactor to


Ring sparger
produce the bubbles.

Agitator is a term for person that actively supports


Agitator some ideology or movement with speeches and
especially actions.

Shaft is component in bioreactor at the agitator and


Shaft
impeller to hold or mix the mixture.

This is the component part of electrode to pass through


pO2 electrode
the oxygen to the bioreactor.
Foam sensor It is a device to sensor the bubble foaming.
Exhaust Is a components part of bioreactor to cooling the
cooler bioreactor by absorbing lots of the heat at bioreactor.

Jacket water Jacket water inlet is a component part to inlet the water
inlet for cooling the bioreactor.
Anti-foam
Component part to inlet the anti-foam to the bioreactor
inlet
Filter A device to prevent contamination

Experiment 1 Exposure to Bioreactor -2-


Laboratory Report BIO 2073 Bioprocess Plant & Equipment

PART AND ITS FUNCTION

Part Function

Impeller The bubble and mixture mix all around.

pH electrode To measure the pH it is acid or base.

Level sensor To avoid any unstable or over level of mixture.

Air inlet To supply air for fermentation process.

Ring sparger For blasting air in for mixing.

A stick to rotate the impeller in order to mix the


Agitator
mixture.
Shaft To hold the impeller and it is rod of mixing as well.

pO2 electrode To measure oxygen in the bioreactor.


Foam control to detected using two conductivity or
Foam sensor
level process.
Exhaust cooler As raw thermal mass to absorb lots of heat.
Jacket water Circulates cooling water to remove the heat produced
inlet by respiration. Prevents denaturizing of enzymes.
Anti-foam inlet Reducing surface elasticity.
Bottom filter: To avoid the microbe in the bioreactor
pass through.
Filter
Top filter: To avoid any such of microbe enter in the
bioreactor.

Experiment 1 Exposure to Bioreactor -3-


Laboratory Report BIO 2073 Bioprocess Plant & Equipment

AIR LIFT BIOREACTOR

1Sections 2Function

3Air-riser 4Sparged region/mixing with air

5Down-comer 6Downward direction/cycle reaction or mixing broth

Add volume to the reactor, reduce foaming and


7Disengageme
minimize recirculation of bubbles through the down
nt zone
comer.

Increase mixing through the reactor


8Draft tube Reduce bubble coalescence.
Equalize shear forces throughout the reactor

Sparge ring Air inlet/ bubble inlet into reactor

Experiment 1 Exposure to Bioreactor -4-