CARBOHYDRATES

MONOSACCHARIDES
DISACCHARIDES
DISACCHARIDES
DISACCHARIDES
STARCH
STARCH
 MOLISCH TEST
Reagents: Concd H2SO4, a-naphthol

(+) visible Purple color at the interface

result:

Test for: carbohydrates

 MOLISCH TEST

http://www.esu.edu/~scady/Experiments/Carbohydrates%28summer%29.pdf
IODINE TEST
Reagents: Iodine-KI solution
(+) visible Blue-black solution
result:
Test for: Starch
BENEDICTS TEST
Reagents: CuSO4, Na2CO3, Na citrate
(+) visible Brick-red ppt (Cu2O)
result:
Test for: Reducing sugars:
monosaccharides
disaccharides
BENEDICTS TEST
BARFOEDS TEST
Reagents: Cu(CH3COO)2, acetic acid
(+) visible Brick-red ppt (Cu2O)
result:
Test for: Monosaccharide (<3 mins),
disaccharides (~10 mins)
MUCIC ACID TEST
Reagents: Hot concd HNO3
(+) visible Broken glass-like crystals
result: (galactaric acid)
Test for: Galactose
 MUCIC ACID TEST

Biochemistry
By Debajyoti Das
BIALS ORCINOL TEST
Reagents: Orcinol, FeCl3, HCl
(+) visible Bluish-green solution
result:
Test for: pentoses
SELIWANOFF TEST

Reagents: Resorcinol, HCl

(+) visible Cherry-red solution
result:
Test for: ketohexoses
PHENYLHYDRAZONE TEST

Biochemistry
By Debajyoti Das

Reducing sugar + phenylhydrazine = phenylhydrazone

Phenylhydrazone + 2 phenylhydrazine = osazone (yellow
or orange crystals)
Laboratory Manual for Practical Biochemistry
By Y. M. Shivaraja Shankara
GLUCOSE
FRUCTOSE
MANNOSE
Laboratory Manual for Practical Biochemistry
By Y. M. Shivaraja Shankara
Laboratory Manual for Practical Biochemistry
By Y. M. Shivaraja Shankara
 NELSON ASSAY
Reagents:
Nelson A: Na2CO3, NaKtartrate, NaHCO3, Na2SO4
Nelson B: CuSO45H2O, H2SO4
Arsenomolydate reagent: (NH4)2MoO4, H2SO4,
NaHAsO47H20
(+) visible Blue color of complex
result:
Test for: Reducing sugars: monosaccharides
and disaccharides