Cell Cultures and Characteristics - Section 1

Cell Cultures and Characteristics - Section 1

1. Which of the following bacteria can grow in acidic pH?
A
Vibrio cholerae
.

B
Lactobacilli
.

C
Shigella
.

D
Salmonella
.
Answer: Option B
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2. Cell size and cell number can be measured using

A
hemocytometer
.

B
coulter counter
.

C
petroff-hausser counting chamber
.

D
none of these
.
Answer: Option B
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3. Which of the following is used for the proper maintenance and preservation of pure
cultures?
A
Periodic transfer to fresh media
.

B
Preservation by overlaying cultures with mineral oil
.

C
Preservation by lyophilization
.

D
All of the above
.
Answer: Option D
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4. In the process of freeze drying, a dense cell suspension is placed in small vials and
is frozen at
A
-60 to -78C
.

B
-20 to -30C
.

C
-30 to -48C
.

D
-48 to -58C
.
Answer: Option A
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5. The solidifying agent commonly used in preparation of media is/are

A
agar
.

B
silica gel
.

C
both (a) and (b)
.

D
none of these
.
Answer: Option C
Explanation:
6. Which of the following chemotherapeutic agents may affect the growth
of Campylobacter jejuni?
A
Vacomycin
.

B
Polymyxin
.

C
Trimethoprim
.

D
None of these
.
Answer: Option D
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7. To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is
maintained at a pH of
A
8.5
.

B
8.90
.

C
6.79
.

D
3.5
.
Answer: Option A
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8. For the selection of endospore-forming bacteria, a mixed culture can be heated at

A
80C for 10 minutes
.

B
70C for 10 minutes
.

C
60C for 10 minutes
.

D
90C for 10 minutes
.
Answer: Option A
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9. The concentration of agar to obtain semi solid media is

A
1.5-20%
.

B
0.5% or less
.

C
>10%
.

D
>10%but<20%
.
Answer: Option B
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10. Which of the following bacteria dies quickly after drying?
A
Treponema pallidium
.

B
Mycobacterium tuberculosis
.

C
Staphylococcus aureus
.

D
Pseudomonas aeruginosa
.
Answer: Option A
13. Agar is used for solidifying culture media because
A
it does not affect by the growth of bacteria
.

B
it does not add to the nutritive properties of the medium
.

C
the melting and solidifying points of agar solution are not the same
.

D
all of the above
.
Answer: Option D
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14. For the selection of lactobacilli present in cheddar cheese, the pH of the medium is
maintained at
A
6.35
.

B
5.35
.

C
4.35
.

D
5.75
.
Answer: Option B
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15. A disease producing species occurring in a mixed culture can often be selected on
the basis of its
A
pathogenic properties
.
 B
special carbon source
.

C
special nitrogen source
.

D
none of these
.
Answer: Option A

16. Important example(s) of enriched medium/media is/ are

A
Loeffler's serum slope
.

B
Bordet-gengou medium
.

C
Blood agar
.

D
All of these
.
Answer: Option D
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17. Cell counting can be carried out by

A
direct microscopic count using petroffhansser counting chamber
.

B
plate counting
.

C
membrane filter count
.

D
all of the above
.
Answer: Option D
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18. Which of the following techniques may be performed quantitatively to determine the
number of bacteria of a particular type?
A
Pour plate
.

B
Spread plate
.
 C
Both (a) and (b)
.

D
Streak plate technique
.
Answer: Option C
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19. Intestinal bacteria can grow in the presence of __________ whereas nonintestinal
bacteria are usually inhibited.
A
bile salts
.

B
low concentration of various dyes
.

C
sugars
.

D
low levels of nitrogen
.
Answer: Option A
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20. Which of the following problem makes it impossible to satisfy all of Koch's
postulates?
A
Microorganism causes serious symptoms in humans
.

B
Microorganism cannot be isolated in pure culture
.

C
Species of microorganism is in question
.

D
Genes from the microorganism cannot be amplified by PCR
.
Answer: Option B