Study/Article

Methods, Findings, Data, Facts, Quotations, Figures, etc. from the Article/Study (include page number)
Abbre
Autho Remaining Questions or Relation to Review
r
viated Study Question/Purpose Methods & Usefulness Conclusions or Findings
Title Uncertainties Topic
CRISPR/cas9 tends to
cut off target, meaning
it deletes/inserts
information in the
wrong place. This is a
Can genetic disease be big concern because it
eradicated using can cause mutations
CRISPR/cas9 on human Otiendo focuses on Even though CRISPR has great potential to and make matters
embryos? And what are genetically engineering genetically modify genetic diseases and worse. Scientist still Ethics concerning the
CRISP
the ethical concerns with the human genome many other great aspects in medicine, there need further research to future potential of
Otien R/Cas genetically modifying a using CRISPR/cas9 (pg 1- is still great concern with the uncertainty of apply this method in a CRISPR and how far
o, Mo 9 HGE human embryo? 2) genetically modifying a human embryo. more accurate manner. it will go.
Long et al., injected
mice at different muscle
locations with a vector
containing CRISPR/cas9.
Postn They tested pre and post Long et. al., found a significant regain of There are many
atal
Geno muscle function by grip muscle function, indicating that How can scientists negative
me strength, PCR and gel CRISPR/cas9, was successful enough to help make these edits connotations with
editin electrophoresis, and IM these mice get their movement back, permanent, and how CRISPRs methods
g How effective is injections, they tested however some rats did not have permanent can they reduce off and if it made off
Chen partial
gzu ly
CRISPR/cas9 at editing these mice at different use of their muscles. Researchers also target edits to avoid target mutations,
Long restor mice genome to rid it of life stages, 4,8 and 12 noticed off target edits which raised a big further mutations in the how would science
et al. es... DMD? (pg 1) weeks. concern. genome. recover.
Ishii et al., bring up a point that IVF was
Germl
ine once thought to be an unethical application
geno and receive crucial criticism but is now a
me- common procedure that has helped many
editin conceive. Even though a call for a How CRISPR/cas9
g
resear
Call for a moratorium on moratorium has been made, many should be applied? How
ch human genome countries allow research on human regulated should
and engineering to set rules embryos, with some regulations already in research on human
its and guidelines for human Ishii and his team place. Communication among the science embryos be? Should we
socioe
Ishii, thical
embryo research, clinical focused on CRISPR/cas9 community would be great, but also limit use to clinical
Tetsu implic applications, and socio- for the editing of human informing the public about such technology applications only or Ethical issues facing
ya ations ethical implications embryos. would be a vital step as well. commercial as well? genetic engineering
 CRISP
R/cas
9
syste
m as
an
Innov CRISPR is still in its
ative Researchers optimized beginning stages and
Genet the complimentary Much research needs to be conducted to in needs to be further
ic
Engin
sequence of the guide order to apply CRISPR/cas9 in an effective Adding modifications on researched without
Jo, Y eering How can off target editing RNA strand to the target manner, so that if may have less of target guide RNA should be being put on a
et al. Tool by CRISPR/cas9 be reduce? sequence. site editing. considered. moratorium.

Fogle CRISP
man, R/Cas
S., 9 and There is no research to I feel that this piece
Santa mitoc They used other prove CRISPR would be shows more of the
na, hondri research to show the There was nothing to conclude since this useful to removing theoretical potential
C., al
Bisho gene
Can CRISPR be a tool to benefit and reliance of was more of an opinion piece. The main mutations so they want that CRISPR is
p, C., replac remove mitochondrial the CRISPR tool for gene message was to promote more research others to try it out to capable of to benefit
Miller ement gene mutations. editing. with CRISPR. see what happens. humans.
The author want scientist
Huma studying or want to study This paper outlines
n CRISPR to think about the They referenced many The ethics of CRISPR the cons of using
Germl ethics in using human regulations that have research leaves a big CRISPR because to
ine zygotes for their research. been placed to slow the question for the become efficient with
Interv
ention
They want to others to pace of the increasing research when using the tool we need a
s consider the full pros of CRISPR research. Their human germlines as it lot of research. So is
Think cons of doing so and what main topic is that if subjects. Even though it beneficial to us
First. it can lead to down the human germline This in an opinion piece but they want they won't live past a moral to use the
Fronti
ers in
future in terms of what is intervention is researchers to ask themselves if they need certain time are we human germlines to
Hildt, Genet allowed in the scientific absolutely necessary for the human zygotes for research before using humans as test master this DNA
E ics community. the research of CRISPR. conducting it. subjects. editing tool.
CRISP This paper is another
R/Cas
Liang, 9- risk for CRISPR use
P., Xu, media because it shows
Y., ted that our knowledge
Zhan gene about it is still not
g, X., editin
Ding, g in Even though CRISPR refined. If used
C., huma hold a lot of potential clinically now it can
Huan n cannot be used cause a lot more
g, R., tripro Unfortunately CRISPR was not deemed correctly yet. We still harm that good. So
Zhan nucle
g, Z., ar
Is CRISPR accurate is They selected a gene to effective in doing its job and it also led to require a lot of research we are still a ways off
Huan zygot editing genes that we want cut and repair within a mutations with the gene that was being before we can perfect before we can use it
g, J es to target? human zygote. edited. its use. in our society.