MSA TEST used for identifying gram + staph or strep cocci

-tests for fermentation of mannitol is selective and differential for

halophiles

Figure 1 MSA TEST YELLOW = POSITIVE, PINK = NEGATIVE

Figure 2 PINK = NO GROWTH, NO FERMENTATION, NEGATIVE

Figure 3 GROWTH AND FERMENTATION OF MANNITOL, POSITIVE

 BLOOD HEMOLYSISused to identify gram + bacteria
Beta hemolysis = complete and full break down of RBC

Figure 4 Beta Hemolysis -- will see a clearing around the streaked areas

Alpha hemolysis = partial breakdown of RBC, will see green tinted areas of covering

Figure 5 Alpha hemolysis

Gamma hemolysis = no breakdown of RBCappears to be unchanged

Bile Esculin Testused to identify gram + bacteria

Black = positive for bile

No color change/clear = negative for bile
E Faecalis black positive for bile

Figure 6 E faecais = positive for bile

S agalactiae = clear/no color change negative for bile

Figure 7 S agalactiae- negative for bile