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Tidewater did not observe any standing water or active water intrusion during the visual
inspection. However, water-stained ceiling tiles were observed in the main office room,
storage room, and Classroom 4 during the inspection conducted on April 7, 2017.
However, all water damaged ceiling tiles had been replaced before the April 10, 2017
site visit;
Dark spots were observed on the bathroom ceiling in Classroom M-22;
Air supply grills with minor rust were observed in Classroom 2, the Diagnosis Room,
Classroom 7B, and Student Project Room #3;
Classroom M-18 was unoccupied at the time of the inspection, and efforts to identify
water infiltration points in the vicinity of the perimeter window seams were underway.
Drywall and wall cavity insulation in these areas had been removed, and the areas
covered with plastic sheeting; and,
The office areas are serviced by ceiling mounted air supply vents and return air grills.
The HVAC systems in most office areas were in operation at the time of the inspection
and the air circulation within most classrooms appeared to be adequate.
Comfort Parameter Air Testing
During the assessment, Tidewater recorded temperature, relative humidity, carbon dioxide
(CO2), and carbon monoxide (CO) measurements in select areas of District Heights Elementary
School using a Metrosonics Indoor Air Quality instrument (Model Number AQ-5000, Serial
Number 2157.) Tidewater recorded these measurements after allowing the device to become
acclimated to the ambient temperature and relative humidity for five (5) minutes. Tidewater
programmed the Metrosonics Indoor Air Quality (IAQ) instrument to sample every 30 seconds
for a period of 10 minutes at each location, and the average concentration of each comfort
parameter was recorded. Tidewater also obtained background samples from outside the main
entrance to the school building each day for comparison.
The results of the IAQ comfort parameter monitoring are provided in Table 1, in Appendix A.
According to the American Society for Heating, Refrigerating and Air Conditioning Engineers
(ASHRAE) guidelines Standard 62.1 2016, the temperature range in summer months should
be maintained between 73F and 79F for maximum occupant comfort. The ASHRAE guideline
for temperature for winter months is between 68.0F and 74.5F. The indoor temperature levels
within the areas sampled ranged between 66.4F and 78.4F. The background temperature
levels outside the building ranged between 51.4F and 76.2. The majority of the temperature
levels recorded within the indoor locations were within the ASHRAE recommended guidelines
for comfort for the spring transitional season.
Per the same guideline, a maximum recommended relative humidity level of 65.0% is
recommended to reduce the likelihood of condensation on cold surfaces. Relative humidity
levels within the indoor locations ranged between 26.6% and 45.3%. The background relative
humidity levels ranged between 28.0% and 40.0% outside the building. The relative humidity
readings in the indoor locations were below the ASHRAE recommended maximum relative
humidity level of 65%.
ASHRAE Standard 62.1 2016 recommends that indoor CO2 levels not exceed 700 ppm above
the outdoor background CO2 level. The CO2 levels in the indoor locations ranged between 477
Page 2 of 5
ppm to 1,447 ppm. The background CO2 levels outside the building ranged between 420 ppm
and 517 ppm. The CO2 levels within Classrooms 5 exceeded 700 ppm above the outdoor
background CO2 level (517), which is the maximum ASHRAE recommended guideline for CO2
in indoor environments. The elevated CO2 levels in this classroom indicate inadequate air
supply to this classroom. There were over 20 students in this classroom during the inspection.
Tidewater did not detect CO in all indoor areas assessed, and therefore were below the
maximum guideline of 9 ppm recommended by the Indoor Air Quality Association (IAQA) for CO
in occupied indoor environments.
Particulate Sampling for Total (Nuisance) Dust
Particulate matter sampling for total dust indicate that the total particulate levels in all locations
sampled were below the OSHA 8-hour Permissible Exposure Limit (PEL) of 15 mg/m3 for total
(nuisance) dust levels in office environments.
Direct read measurements for total (nuisance) dust are summarized on Table 2, in Appendix A.
Spore Trap Bioaerosol Sampling
One April 5, 2017, Tidewater collected a total of 22 spore trap air samples using Allegenco-D
cassettes to characterize potential airborne fungal spores within the school building. On April 7,
2017, Tidewater collected a total of 15 samples and on April 10th, 2017 Tidewater collected a
total of 11 samples using Allegenco-D cassettes from representative areas of the building. On
April 11, 2017, Tidewater collected a total of seven (7) samples from areas of the building where
drywall and wall cavity insulation had been removed as part of efforts to identify water infiltration
points in perimeter walls. Background samples were collected outside the building each day of
the survey to provide a baseline for comparison.
Tidewater obtained these samples using Allergenco-D cassettes to characterize potential
airborne fungal spores in the assessed areas. Air samples were collected for total fungal
spores using the pump from a Buck BioAire Bioaerosol Sampling Pump (Pump Model
Number B520 and Serial Number B151491) calibrated to a flow rate of 15.0 Liters per minute.
Each sample was run for a period of five (5) minutes at each location to collect a total sample
volume of 75 liters of air.
Once collected, the samples were transported to EMSL Analytical Laboratory (EMSL) located in
Beltsville, Maryland for analysis with a same day turnaround time. The samples were
transported following rigorous chain-of-custody guidelines to ensure proper handling and
delivery of the samples. EMSL is accredited in the American Industrial Hygiene Association
(AIHA) Environmental Microbiology Laboratory Accreditation Program (EMLAP) and is a
successful participant in AIHAs Environmental Microbiology Proficiency Analytical Testing
(EMPAT) program (Laboratory Number 102891.)
The samples were analyzed via light microscopy at the standardized magnification of 600X.
This technique does not allow for the differentiation between Aspergillus and Penicillium spores
because they are morphologically identical. Additionally, the technique does not allow for
cultivation, or the identification of spores to the species level, except in a few cases.
There are no universally accepted federal or State of Maryland standards for acceptable
airborne concentrations of bioaerosols in an indoor occupational environment. In general,
airborne concentrations indoors should be less than that found in the outdoor air, with similar
Page 3 of 5
species composition. Indoor spore counts significantly greater than those outdoors, or the
presence of large numbers of different types of spores indoors that are not found outdoors, may
indicate contamination and potential indoor air quality problems.
The total mold spore counts in all samples obtained from selected areas of the building on April
11, 2017 ranged between 240 and 1,340 spores per cubic meter (spores/m3). The total mold
spore concentration in the outdoors (background) sample was 1,540. The total mold spore
concentrations in all indoor locations sampled on April 11, 2017 were below the outdoors
(background) sample total count of 1,540 spores/m3. No significant concentrations of individual
fungal species were identified in the assessed indoor area samples. These results do not
suggest the presence of a significant indoor source of fungal contamination that would
adversely affect indoor air quality within the sampled areas.
The summary of the results for the spore trap sampling are provided in Table 3 in Appendix A.
The laboratory analytical results, including speciation and chain of custody forms for the spore
trap samples are included as Attachment B.
Conclusions
Based on this IAQ assessment, Tidewater offers the following conclusions:
Tidewaters visual inspection of the select occupied areas of the District Heights
Elementary School did not reveal any visible evidence of ongoing water intrusion on the
walls, floors or ceiling in any of the office areas inspected. However, stained ceiling tiles,
indicating signs of past water intrusion were observed in the Main Office, Storage Room,
and Classroom 4. These areas had been abated, cleaned and sanitized, and the ceiling
tiles replaced by the end of our assessment;
The average temperature readings in the select indoor areas ranged between 66.4F
and 78.4F. Majority of these occupied spaces had temperature levels within the
ASHRAE recommended comfort guidelines for the spring transitional season;
The average relative humidity levels in the select indoor areas ranged between 26.6%
and 45.3%. These relative humidity levels were below the ASHRAE recommended
maximum relative humidity level of 65%;
The CO2 levels within Classrooms 5 exceeded 700 ppm above the outdoor background
CO2 level (517 ppm), which is the maximum ASHRAE recommended guideline for CO2
in indoor environments. The elevated CO2 levels indicate inadequate air supply to this
classroom;
Tidewater did not detect CO in all indoor locations assessed;
Particulate sampling for total (nuisance) dust analytical results are all of the samples
collected were below the OSHA 8-hour Permissible Exposure Limit (PEL) of 15 mg/m3
for total (nuisance) dust; and
Total spore trap air sampling results do not indicate the presence of a significant indoor
source of fungal contamination that would adversely affect indoor air quality within the
assessed areas of the building.
Page 4 of 5
Recommendations
Based on these findings, Tidewater recommends the following steps:
Clean the bathroom ceiling in classroom M-22 with kitchen/bathroom sanitizing solution
to remove the dark spots observed on the ceiling. If staining of the surface remains, the
area should be repainted with a fungal-resistant paint. Also, ensure proper bathroom
exhaust operation to help reduce humidity and the resulting condensation in the room.
Adjust the HVAC system in Classroom 5 to provide adequate conditioned air for the
anticipated occupancy of the area. This will help lower the overall CO2 levels to a
standard below 700 ppm above the background levels.
Qualifications
Tidewater has endeavored to investigate existing conditions at the District Heights Elementary
School located at 2200 Country Road, District Heights, Maryland as they pertain to indoor air
quality. Our conclusions and recommendations are based on the observations made on the
days of our assessment, laboratory data from the time of the assessment, and information
provided by both our Client and the area occupants. Actual conditions vary from day to day
throughout the year.
Tidewater appreciates the opportunity to provide consulting services for Prince Georges County
Public Schools. Please contact us should any questions arise concerning this report or if we
may be of further assistance.
Sincerely,
Tidewater, Inc.
Skanda Abeyesekere, MS, CIH, CSP, CHMM Jonathan N. Schatz, MS, CES, CEI
Project Manager Manager, IH Services
SA/JNS
Page 5 of 5
Attachment A
April 5, 2017
April 7, 2017
Concentration (mg/m3)
Location
Respirable Total Dust
PM2.5
Dust (Nuisance)
April 5, 2017
Classroom 4 0.005 0.006 0.010
Classroom 1 0.000 0.000 0.000
Classroom 2 0.003 0.003 0.003
Classroom 3 0.008 0.008 0.021
Classroom 3 (Study Rm 1) 0.004 0.004 0.004
Student Project Room #1 0.005 0.006 0.009
Student Project Room #2 0.003 0.003 0.005
Classroom 4 (Study Rm 2) 0.008 0.008 0.008
Classroom 5 0.007 0.009 0.014
Classroom 6 0.006 0.006 0.016
Diagnosis Room 0.006 0.006 0.012
Classroom 7B 0.008 0.008 0.017
Classroom 7 0.009 0.009 0.013
Classroom 7A 0.007 0.007 0.017
Classroom M 18 0.011 0.011 0.023
Classroom M 20 0.015 0.015 0.020
Classroom M22 0.006 0.006 0.012
Classroom M23 0.008 0.008 0.015
Classroom M21 0.005 0.005 0.010
Classroom M19 0.009 0.009 0.018
Gymnasium 0.013 0.013 0.018
Background (Outdoors) 0.005 0.005 0.007
Table 2: Particulate Matter Concentration
District Heights Elementary School
Concentration (mg/m3)
Location
Respirable Total Dust
PM2.5
Dust (Nuisance)
April 7, 2017
Pre-K Classroom 2 0.001 0.002 0.004
Pre-K Classroom 1 0.005 0.006 0.008
Storage Room 0.004 0.004 0.012
Classroom 9 0.036 0.038 0.055
Classroom 11 0.001 0.001 0.005
Student Project Room #3 0.004 0.004 0.005
Art Room 0.003 0.002 0.005
Classroom 13 0.000 0.000 0.004
Classroom 15 0.001 0.001 0.010
Classroom 17 0.011 0.000 0.000
Classroom 14 0.001 0.001 0.004
Classroom 12 0.015 0.015 0.021
Classroom 10 0.004 0.001 0.009
Classroom 8 0.000 0.005 0.001
Background (Outdoors) 0.000 0.000 0.000
Table 2: Particulate Matter Concentration
District Heights Elementary School
Concentration (mg/m3)
Location
Respirable Total Dust
PM2.5
Dust (Nuisance)
April 5, 2017
April 7, 2017
Sample Total Fungi
Sample Number Sample Location Volume Concentration
(L) (Counts/m3)
AIR-1 Pre-K Classroom 2 75 730
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria 1* 10* 0.3 - - - - - -
Ascospores 12 530 16.4 4 200 31.3 4 200 28.6
Aspergillus/Penicillium 4 200 6.2 2 90 14.1 4 200 28.6
Basidiospores 44 2000 61.9 7 300 46.9 4 200 28.6
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 11 490 15.2 1 40 6.3 2 90 12.9
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - 1* 10* 1.6 1* 10* 1.4
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium - - - - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 72 3230 100 15 640 100 15 700 100
Hyphal Fragment - - - - - - 1* 10* -
Insect Fragment - - - - - - - - -
Pollen 1* 10* - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 3 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 1 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria 1 40 2.4 - - - - - -
Ascospores 9 400 23.7 2 90 12.2 12 530 28.2
Aspergillus/Penicillium 1 40 2.4 5 200 27 2 90 4.8
Basidiospores 21 930 55 9 400 54.1 27 1200 63.8
Bipolaris++ 1 40 2.4 - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 3 100 5.9 1 40 5.4 1 40 2.1
Curvularia 1 40 2.4 1* 10* 1.4 1* 10* 0.5
Epicoccum 1* 10* 0.6 - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 2 90 5.3 - - - 1* 10* 0.5
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium - - - - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 40 1690 100 18 740 100 44 1880 100
Hyphal Fragment 2* 30* - - - - - - -
Insect Fragment - - - - - - - - -
Pollen - - - 1 40 - 1 40 -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 3 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 1 40 4.4 - - - 4 200 21.5
Aspergillus/Penicillium 3 100 11.1 1 40 5.6 1 40 4.3
Basidiospores 12 530 58.9 15 670 93.1 11 490 52.7
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 4 200 22.2 1* 10* 1.4 5 200 21.5
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 2* 30* 3.3 - - - - - -
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium - - - - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 22 900 100 17 720 100 21 930 100
Hyphal Fragment 2 90 - - - - 4 200 -
Insect Fragment - - - - - - - - -
Pollen - - - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 2 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 1 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - 2 90 4.9 1 40 3.6
Ascospores 1 40 5.3 7 300 16.2 6 300 27
Aspergillus/Penicillium 5 200 26.7 1 40 2.2 2 90 8.1
Basidiospores 8 400 53.3 11 490 26.5 10 440 39.6
Bipolaris++ - - - 1 40 2.2 - - -
Chaetomium - - - - - - - - -
Cladosporium 2 90 12 18 800 43.2 4 200 18
Curvularia - - - - - - - - -
Epicoccum - - - 1 40 2.2 - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 1* 10* 1.3 1 40 2.2 1 40 3.6
Pithomyces 1* 10* 1.3 1* 10* 0.5 - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium - - - - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 18 750 100 43 1850 100 24 1110 100
Hyphal Fragment 1 40 - - - - 1 40 -
Insect Fragment - - - - - - - - -
Pollen - - - 2 90 - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - 1* 10* 1.1 1* 60* 1.1
Ascospores 5 200 10.5 3 100 10.6 5 1000 18.2
Aspergillus/Penicillium 8 400 21.1 - - - 2 400 7.3
Basidiospores 9 400 21.1 9 400 42.6 5 1000 18.2
Bipolaris++ - - - 1* 10* 1.1 - - -
Chaetomium - - - - - - 1* 60* 1.1
Cladosporium 20 890 46.8 8 400 42.6 12 2500 45.6
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 1* 10* 0.5 1* 10* 1.1 1* 60* 1.1
Pithomyces - - - 1* 10* 1.1 - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - 2 400 7.3
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium - - - - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 43 1900 100 24 940 100 29 5480 100
Hyphal Fragment 1 40 - 2 90 - - - -
Insect Fragment - - - 1 40 - - - -
Pollen - - - 1* 10* - 1* 60* -
Analyt. Sensitivity 600x - 44 - - 44 - - 208 -
Analyt. Sensitivity 300x - 13* - - 13* - - 62* -
Skin Fragments (1-4) - 4 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - 1* 10* 0.7 2 90 8
Ascospores 11 490 14.5 4 200 14.5 2 90 8
Aspergillus/Penicillium 1 40 1.2 2 90 6.5 1 40 3.6
Basidiospores 20 890 26.3 8 400 29 8 400 35.7
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 40 1800 53.1 14 620 44.9 10 440 39.3
Curvularia - - - - - - 1* 10* 0.9
Epicoccum 1 40 1.2 - - - 1* 10* 0.9
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 2 90 2.7 - - - - - -
Pithomyces - - - 1* 10* 0.7 - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - 1 40 2.9 - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium 1 40 1.2 - - - 1 40 3.6
Oidium - - - - - - - - -
Spegazzinia - - - 1* 10* 0.7 - - -
Sporidesmiella - - - - - - - - -
Total Fungi 76 3390 100 32 1380 100 26 1120 100
Hyphal Fragment 1 40 - 1 40 - 2 90 -
Insect Fragment - - - - - - - - -
Pollen 1 40 - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 1 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 13 580 15.6 5 200 21.5 3 100 9
Aspergillus/Penicillium 6 300 8.1 1 40 4.3 4 200 18
Basidiospores 42 1900 51.2 5 200 21.5 11 490 44.1
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 20 890 24 11 490 52.7 4 200 18
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - 1 40 3.6
Myxomycetes++ - - - - - - 5* 70* 6.3
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - 1* 10* 0.9
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Arthrinium - - - - - - - - -
Oidium 1 40 1.1 - - - - - -
Spegazzinia - - - - - - - - -
Sporidesmiella - - - - - - - - -
Total Fungi 82 3710 100 22 930 100 29 1110 100
Hyphal Fragment 1 40 - 1* 10* - 2 90 -
Insect Fragment - - - - - - 1 40 -
Pollen 1* 10* - - - - 9 400 -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 3 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 3 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Page 1 Of 2
OrderID: 191703862
Page 2 Of 2
EMSL Analytical, Inc. Order ID: 191703940
10768 Baltimore Avenue Beltsville, MD 20705 Customer ID: TIDE50
Phone/Fax: (301) 937-5700 / (301) 937-5701 Customer PO:
http://www.EMSL.com / beltsvillelab@emsl.com Project ID:
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 1* 10* 1.4 1 40 3.3 1 40 1.8
Aspergillus/Penicillium 12 530 72.6 16 710 58.2 31 1400 64.2
Basidiospores 2 90 12.3 2 90 7.4 6 300 13.8
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 3 100 13.7 1 40 3.3 8 400 18.3
Curvularia - - - 1 40 3.3 - - -
Epicoccum - - - 2 90 7.4 - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - 2 90 7.4 1 40 1.8
Pithomyces - - - - - - - - -
Rust - - - 1 40 3.3 - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - 1 40 3.3 - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium - - - - - - - - -
Spegazzinia - - - 1 40 3.3 - - -
Total Fungi 18 730 100 28 1220 100 47 2180 100
Hyphal Fragment 1 40 - 1 40 - - - -
Insect Fragment - - - - - - - - -
Pollen - - - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 4 - - 3 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - 1 40 10.8 - - -
Ascospores 1* 10* 1 - - - - - -
Aspergillus/Penicillium 9 400 38.5 2 90 24.3 6 300 54.5
Basidiospores 4 200 19.2 5 200 54.1 1 40 7.3
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 7 300 28.8 1 40 10.8 5 200 36.4
Curvularia - - - - - - - - -
Epicoccum 2 90 8.7 - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 1 40 3.8 - - - - - -
Pithomyces - - - - - - - - -
Rust - - - - - - 1* 10* 1.8
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium - - - - - - - - -
Spegazzinia - - - - - - - - -
Total Fungi 24 1040 100 9 370 100 13 550 100
Hyphal Fragment - - - - - - 1 40 -
Insect Fragment 1* 10* - - - - - - -
Pollen - - - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 2 - - 1 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 1 40 11.1 - - - 1* 10* 4.8
Aspergillus/Penicillium 2 90 25 3 100 71.4 3 100 47.6
Basidiospores 3 100 27.8 - - - 3 100 47.6
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 2 90 25 - - - - - -
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 2* 30* 8.3 1 40 28.6 - - -
Pithomyces - - - - - - - - -
Rust 1* 10* 2.8 - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium - - - - - - - - -
Spegazzinia - - - - - - - - -
Total Fungi 11 360 100 4 140 100 7 210 100
Hyphal Fragment - - - 1 40 - 1 40 -
Insect Fragment - - - - - - - - -
Pollen - - - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 1 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores - - - 4 200 17.4 2 90 19.1
Aspergillus/Penicillium 1 40 21.1 5 200 17.4 1 40 8.5
Basidiospores 2 90 47.4 6 300 26.1 3 100 21.3
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 4* 50* 26.3 10 440 38.3 - - -
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - 1* 10* 0.9 5 200 42.6
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium 1* 10* 5.3 - - - 3* 40* 8.5
Spegazzinia - - - - - - - - -
Total Fungi 8 190 100 26 1150 100 14 470 100
Hyphal Fragment - - - 1 40 - 1 40 -
Insect Fragment - - - - - - - - -
Pollen - - - - - - - - -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 1 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 2 -
Background (1-5) - 1 - - 2 - - 2 -
Sample Comments: 191703940-0011 Sample contains RARE amount of algae-like organisms.
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 1 40 4.9 - - - 3 100 11.9
Aspergillus/Penicillium 11 490 59.8 2 90 23.1 5 200 23.8
Basidiospores 5 200 24.4 4 200 51.3 5 200 23.8
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 2 90 11 3 100 25.6 6 300 35.7
Curvularia - - - - - - - - -
Epicoccum - - - - - - 1 40 4.8
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - - - - - - -
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium - - - - - - - - -
Spegazzinia - - - - - - - - -
Total Fungi 19 820 100 9 390 100 20 840 100
Hyphal Fragment 2 90 - - - - - - -
Insect Fragment - - - - - - - - -
Pollen - - - - - - 2 90 -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 4 - - 1 - - 1 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 1 - - 1 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Page 1 Of 2
OrderID: 191703940
Page 2 Of 2
EMSL Analytical, Inc. Order ID: 191704002
10768 Baltimore Avenue Beltsville, MD 20705 Customer ID: TIDE50
Phone/Fax: (301) 937-5700 / (301) 937-5701 Customer PO:
http://www.EMSL.com / beltsvillelab@emsl.com Project ID:
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores - - - - - - 2 90 15.8
Aspergillus/Penicillium - - - 1 40 33.3 - - -
Basidiospores 8 300 49.2 1 40 33.3 1 40 7
Bipolaris++ 1* 10* 1.6 - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 7 300 49.2 1 40 33.3 9 400 70.2
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - - - - 1 40 7
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Botrytis - - - - - - - - -
Trichocladium - - - - - - - - -
Total Fungi 16 610 100 3 120 100 13 570 100
Hyphal Fragment 1 40 - 1 40 - - - -
Insect Fragment 1* 10* - - - - - - -
Pollen 3* 40* - 1* 10* - 1 40 -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 2 - - 2 - - 1 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 2 - - 1 - - 1 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores - - - 1 40 13.3 1 40 1.6
Aspergillus/Penicillium - - - 1 40 13.3 45 2000 78.4
Basidiospores 2 90 90 2 90 30 4 200 7.8
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium - - - 2 90 30 6 300 11.8
Curvularia - - - - - - - - -
Epicoccum - - - - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - 2* 30* 10 1* 10* 0.4
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - 1* 10* 3.3 - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Botrytis - - - - - - - - -
Trichocladium 1* 10* 10 - - - - - -
Total Fungi 3 100 100 9 300 100 57 2550 100
Hyphal Fragment - - - 1 40 - 2 90 -
Insect Fragment - - - - - - - - -
Pollen 1 40 - 1* 10* - 3* 40* -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 3 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 2 90 6.4 2 90 32.1 1 40 11.4
Aspergillus/Penicillium 28 1200 85.1 1 40 14.3 - - -
Basidiospores 3 100 7.1 3 100 35.7 6 300 85.7
Bipolaris++ 1* 10* 0.7 - - - - - -
Chaetomium - - - - - - - - -
Cladosporium - - - 1 40 14.3 - - -
Curvularia - - - - - - - - -
Epicoccum 1* 10* 0.7 - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ - - - 1* 10* 3.6 1* 10* 2.9
Pithomyces - - - - - - - - -
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Botrytis - - - - - - - - -
Trichocladium - - - - - - - - -
Total Fungi 35 1410 100 8 280 100 8 350 100
Hyphal Fragment 1* 10* - - - - - - -
Insect Fragment - - - - - - - - -
Pollen - - - 1 40 - 1* 10* -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 3 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 1 - - 1 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
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OrderID: 191704002
Page 2 Of 2
EMSL Analytical, Inc. Order ID: 191704047
10768 Baltimore Avenue Beltsville, MD 20705 Customer ID: TIDE50
Phone/Fax: (301) 937-5700 / (301) 937-5701 Customer PO:
http://www.EMSL.com / beltsvillelab@emsl.com Project ID:
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - - - - - - -
Ascospores 1 40 16.7 - - - - - -
Aspergillus/Penicillium - - - 6 300 85.7 15 670 73.6
Basidiospores 2 90 37.5 - - - 3 100 11
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium - - - 1 40 11.4 2 90 9.9
Curvularia - - - - - - - - -
Epicoccum 1 40 16.7 - - - - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 2* 30* 12.5 - - - 1* 10* 1.1
Pithomyces - - - - - - 1 40 4.4
Rust - - - - - - - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - 1* 10* 2.9 - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium 1 40 16.7 - - - - - -
Total Fungi 7 240 100 8 350 100 22 910 100
Hyphal Fragment 1 40 - 1* 10* - - - -
Insect Fragment - - - - - - - - -
Pollen 1 40 - 3* 40* - 3 100 -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 3 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 1 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Spore Types Raw Count Count/m % of Total Raw Count Count/m % of Total Raw Count Count/m % of Total
Alternaria - - - 2* 30* 2.2 - - -
Ascospores - - - 2 90 6.7 - - -
Aspergillus/Penicillium 4 200 44.4 9 400 29.9 6 300 55.6
Basidiospores 5 200 44.4 7 300 22.4 - - -
Bipolaris++ - - - - - - - - -
Chaetomium - - - - - - - - -
Cladosporium 1 40 8.9 6 300 22.4 4 200 37
Curvularia - - - - - - - - -
Epicoccum - - - 1* 10* 0.7 - - -
Fusarium - - - - - - - - -
Ganoderma - - - - - - - - -
Myxomycetes++ 1* 10* 2.2 1 40 3 3* 40* 7.4
Pithomyces - - - 1 40 3 - - -
Rust - - - 2 90 6.7 - - -
Scopulariopsis - - - - - - - - -
Stachybotrys - - - - - - - - -
Torula - - - - - - - - -
Ulocladium - - - - - - - - -
Unidentifiable Spores - - - - - - - - -
Zygomycetes - - - - - - - - -
Arthrinium - - - 1 40 3 - - -
Total Fungi 11 450 100 32 1340 100 13 540 100
Hyphal Fragment - - - 3* 40* - 1 40 -
Insect Fragment - - - - - - - - -
Pollen 4 200 - 4 200 - 1* 10* -
Analyt. Sensitivity 600x - 44 - - 44 - - 44 -
Analyt. Sensitivity 300x - 13* - - 13* - - 13* -
Skin Fragments (1-4) - 3 - - 4 - - 4 -
Fibrous Particulate (1-4) - 1 - - 1 - - 1 -
Background (1-5) - 1 - - 2 - - 2 -
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
Bipolaris++ = Bipolaris/Drechslera/Exserohilum
Myxomycetes++ = Myxomycetes/Periconia/Smut
Stefanie Schneider, Microbiology Lab Manager
No discernable field blank was submitted with this group of samples.
or Other Approved Signatory
High levels of background particulate can obscure spores and other particulates leading to underestimation. Background levels of 5 indicate an overloading of background particulates, prohibiting accurate
detection and quantification. Present = Spores detected on overloaded samples. Results are not blank corrected unless otherwise noted. The detection limit is equal to one fungal spore, structure, pollen, fiber
particle or insect fragment. "*" Denotes particles found at 300X. *-* denotes not detected. EMSL maintains liability limited to cost of analysis. This report relates only to the samples reported above and may not
be reproduced, except in full, without written approval by EMSL. EMSL bears no responsibility for sample collection activities or analytical method limitations. Interpretation and use of test results are the
responsibility of the client. Samples received in good condition unless otherwise noted.
Samples analyzed by EMSL Analytical, Inc. Beltsville, MD AIHA-LAP, LLC--EMLAP Lab 102891
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