INTRODUCTION RNA plays a major role in protein synthesis

as it is involved in the transcription ,

Nucleic acids are molecules that allow
decoding, and translation of the genetic
organisms to transfer genetic information
code to produce proteins. There are three
from one generation to next.[1] Nucleic
types of RNA that are involved in protein
acids are divided into two main structural
synthesis. The mRNA or the messenger RNA
classes: deoxyribonucleic acid (DNA) and
which plays an important role in the
ribonucleic acid (RNA) which made up of
transcription of DNA. The tRNA or the
nitrogenous bases ,sugar and phosphate.
transfer RNA which translate the message
Ribonucleic acid (RNA) is a single stranded within the nucleotide sequence of mRNa into
nucleic acid and made up of long chain specific amino acid sequence.[1]. The rRNA
component called nucleotides. Each or the ribosomal RNA is the one responsible
nucleotide is composed of a phosphate- for the creating peptide bonds between
ribose sugar backbone and nitrogenous amino acids in the polypeptide chain.
bases derived from purines which are
This experiment aim to isolate RNA from
adenine (A) and guanine (G) and from
yeast and determine the purity of the
pyrimidines which are cytosine (C) and uracil
extracted RNA by qualitative characterization
(U). RNA is relatively shorter in molecules
of RNA after the basic hydrolysis.
thus is not easily damaged by shearing but it
is more unstable and more prone to
degradation due to having a ribose sugar.