Protozoa are unicellular eukaryotes that may develop
into cysts in harsh environmental conditions. The main divisions of protozoa are shown in Figure 4.7. Protozoa species may be capable of replicating intracellularly or extracellularly. The most important protozoal disease worldwide is malaria, which is caused by the Plasmodium species. Identification of protozoa often occurs by microscopy of patient specimens. Specimens include faeces, blood and tissue. Culture methods are not routinely applied for identification purposes.