Post Graduate School

Indian Agricultural Research Institute, New Delhi

...,,.,'
Examination f o r Admission to Ph.D. Programme 2011-2012

Discipline : Molecular Biology and Biotechnology

Discipline Code : 14 R o l l NO]
I
L -

Please Note:

(i)This question paper contains 14 pages. Please check whether a l l the pages are
printed in this set. Report discrepancy, if any, immediately to the invigilator.

(ii)There shall be NEGATIVE marking for WRONG answers in the Multiple

Choice type questions (No. I to 130) tvhicli carry 9ne mark each. For every
wrong answer 0.25 mark will be deducted.

5. To add'ress the problems of sustainable and

PART- I(General Agriculture)
Multiple choice questions (No. 1 to 30).
Choose the correct answer (a, b, c or d )
and enter your choice in the circle (by
shadlng with a pencil) on the OMR
answer sheet as per the instructions
given on the answer sheet.
1. Which of the following crops have been
approved for commercial cultivation in lndia?
a) Bt cotton and Bt brinjal
b) Bt cotton and Golden Rice
c) Bt maize and Bt cotton
-d) Bt cotton only
2. This year (2010-11) the expected food grain
production in lndia is
a) 212 million tonnes
b) 220 million tonnes
C) 235 million tonnes
d) 250 million tonnes
3. The genome of which of the following crops
is still not completely sequenced?
a) Rice
b) Soybean
c) Sorghum
d) Wheat
holistic development of rainfed areas,
incllidlng appropriate. farming and livelihood
system approaches, the-Government of lndia
has sei up the
a) National Rainfed Area Authority
- b) National Watershed Development Project for
Rainfed Areas
'
c) National Mission on Rainfed Areas
d) Command Area Development and Water
Management Authority
8. Which of the following sub-schemes are not
covered under the Rashtrlya Krishi Vikas
Yojana?
a) Extending the Green Revolution to eastern
lndia
b) Development of 60,000 pulses and oiiseeds
villages in identified watersheds
c) National Mission on Saffron
d) National Mission on Bamboo
7. The minimum support price for the common
variety of paddy announced by the
Government of lndia for the year 2010-11
was
a) t 1030
b) i l O O O
c) 7 980
d) t 950

4. According to the Approach Paper to the 1 2 ~ 8. According to the Human Development

Five Year Plan, the basic objectwe of the Report 2010 of the United Nations, India's
12'~Plan 1s rank In terms of the human development
a) Inclusive growth index Is
b) Sustainable growth a) 119
c) Faster, more inclusive and sustainable b) 134
growth c) 169
d) Inclusive and sustainable growth d) 182
 Subject : PhD- Molecular Biology and Bioteehnology- 2011 2
. .

9. Which of the following does not apply to SRI 18. Inbred lines that have .same genetic
method of paddy cultivation? constitution but differ only at one locus are
a) Reduced water application called ,
b) Reduced plant density a) Muiti lines
.c) Increased application of chemical fertilizers Ib) Monohybrid
d), Reduced age of seedlings c) lsogenic lines
d) Pure lines
10. Which organic acid, oflen used as a
preservative, occurs naturally in cranberries, 19. For applying 100 kg of nitrogen, how much
prunes, cinnamon a i ~ dcloves? urea would one use?
a) Citric acid .-a) 45 kg
b) Benzoic acid b) I l l k g
c) Tartaric acid
d) Lactic acid
C) 222 kg ,
d) 333 kg
11. Cotton belongs to the family 20. The devastating impact of plant disease on
a) Cruciferae human suffering and survival was first
b) Anacardiaceae realized by epidemic of
c) Malvaceae a) Brown spot of rice in Bengal
d) Solanaceae b) Late blight of potato in USA
12. Photoperiodism is c,) Late blight of potato in Europe
d) Rust of wheatin India
a) Bendihg of shoot towards source of light
,b) Effect of lighvdark durations on. physiological 21, The species of rice (Ow;) other than O,
processes
safiva that is cultiveted is
c) Movement of chloroplast in cell in response
to light a) 0. rufipugon
d) Effect of light on chlorophyll ~ynthesis
ti) 0. lon$isteminata
c) 0. glaberrima
13. Ergot disease is caused bv which Pathogen
- d) 0. nivara
onwhich host?
a) Claviceps purpurea on rye 22. The enzyme responsible for the fixation of
b) Puccinia recondita on wheat C 0 2 In mesophyll cells of C-4 plants 1s
c) Drechlera sorokiniana on wheat a) Malic enzyme
d) Albugo candida on mustard b) Phosphoenol pyruvate carboxylase
c) Phosphoenol pyruvate carboxykinase
14. Rocks are the chief sources of parent d) RuBP carboxylase
materials over which soils are developed.
Granite, an important rock, is classified as 23.Which one of the following is a 'Vertisol'?
a) Igneous rock a) Black cotton soil
b) Metamorphic rock b) Red sandy loam soil
c) Sedimentary rock c) Sandy loam sodic soil
d) Hybrid rock d) Submontane (Tarai) soil

15. Which one of the following is a Kharif crop? 24.What is the most vipjQle physical ,
a) Pearl millet characteristic of cell? in metaphase? :
b) Lentil a) Elongated chromosomes >
c) Mustard b) Nucleus visible but chromosomes not
d) Wh'eat c) Fragile double stranded loose chromosomes
d) Condensed paired chromosomes on the cell
16. ~ h dcoefficient of variation (C.V.) 1s plate
calculated by the formula - .
a) (MeanlS.D.) x 100 25. All weather phenomena like rain, fog and
b) (S.D.iMean) x 100 mist occur in
c) S.D./Mean -aj. Troposphere
d) Mean1S.D. b) Mesosphere
C) Ionosphere
17. Which of the following is commonly referred
d) Ozonosphere
to as muriate of potash?
a) Potassium nitrate
b) Potassium chloride
c) Potassium sulphate
d) Potassium silicate
 -
Subject : PhD Molecular Biology ancl Biotechnology - 2011 3

26. Which of the following elements is common 34. A wild-type allele usu.ally refers to
to all proteins and nucleic acids? a) Dominant allele
a) Sulphur b) Recessive allele
b) Magnesium c) Allele found in high frequency
. c) Nitrogen d) Allele found in wild-related species
d) Phosphorous
35. Which pf the following is a case of reverse
27. Silt has intermediate characteristics between genetics?
a) Sand and loam a) Cloning of a gene by transposon
b) Clay and loam mutagenesis
c) Loam and gravel b) Map-based cloning of a gene for disease
d) Sand and clay resistance
c) Identifibatioh of gene function by over
28. Certified seed Is produced from expression or silencing
a) Nucleus seed d) Isolation
,,_ of
. a gene base_don natural variation
b) Breeder seed
c) Foundation seed 36. A ,gene' that .originates through duplic<ltion
d) Truthful seed within a species is called
a) Paralogous
29. Seedless banana is an b) Heteroiogous
a) Autotriploid c) Orthologous
b) Autotetrapioid d) Homologous
cl Ailotri~loid
dj ~lloteira~loid 1 37. For alants, which of the following is not an
immortal population for genetic s t d e s ?
30. Which one of the following IS used to test the a) NiLs
goodness-of-fit of a distribution? b) RlLs
a) Normal test c) Doubled haploids
b) t-test d) BCtFi
c) Chi-square test
d) F-test 38. A mis-sense mutation leads to
a) Loss of transcription ,
' PART- I1 (Subject Paper) b) Loss of protein production
Multiple choice questions (No. 31 to 130).
Choose the correct answer (a, b, c or d)
1 c)
d)
Production of modified protein
NOchanae in the arimarv structure of Droteln

and enter your choice in the circle (by
shading with a pencll) on the OMR
answer sheet as Der the instructions
given on the answeisheet.
31. Cellulose is a polymer of glucose with
39. A repressor mutation Is
- a) Mutation occurring in another gene- leading
to appearance of wild-type phenotype from a
-
I mutant
b) Mutation in the same gene but at a different
site leading to restoration of wild-type

- a)
b)
linkage.
a-1-4
P-1-4
phenotype
c) Mutation at the same site. leading to
restoration of original gene function
C) a-1-6 d) A mutation in the same gene that prevents
expression of another gene
d) 1)-1-6 , .

32 Centrioles are found in cells of 40. The ?umber of clones required for a true
representative genomic DNA library is
-a) Animals
b) Plants dependent on many factors. Choose the right
c) Fungi czmbination of factors from the following:
d) Bacteria i) Size of the genome
li) No. of genes in the organism
33. Overlapping genes are more often found in iii) Mean s1ze.of the clones
a) Viruses iv) Confidence level (i.e. probability of having
b) Bacteria at least one copy of any DNA sequence
c) Fungi present in the organism)
d) Human beings a) i, ii and iii
b) i, ii and4v

I c)
d)
i,iii and iv
ii,iiiandiv
 Subject : PhD- Molecular Biology and Biotechnology - 2011 4

41. For a given organism, the number of c-DNA 47. Which of the following causes frameshift
mutations?
- libraries that can be constructed is
a) Equal(to the number of genes a) Proflavin '
b) Directly proportionalto the ifedpan of the organism b) 2-Aminopurine
c) Equal to the genomic content c) 5-Bromouracil
d) Unlimited ?
d) Nitrous acid
42. in garden pea, Mendel found that yellow 48. Which of 'the following Is not involved In
seed colour was dominant to green and recombination in eukaryotes?
\\ round seed shape was dominant to a) Rad 51
shrunken. What are the expected ratios of b) Rho
yellow:green and r0und:shrunken c) Dmc 1
phenotypes in the F, of a cross of a pure d) Spo 11 I
yellow, round X green, shrunken?
a) 3:l and 4:l 49. The binding and assembly of Rec A, the
b) 2:l and 3:l central pfotein in recombination in E.coh, is
c) Both 3:l much mordrapid on which of the following'?
d) Both 2.1 a) Single stranded DNA
43. A barn-prath~ading:,..genq. acquired a b) Double stranded DNA
termination codon:,in. the middle, of coding c) Binds with equal rapidity to both stngle and
i8$ton; y e f ' ~ a functio,nai p r o t e i n was double stranded DNA
pfoduced: Which of ttie following could be d) Rec A does not bind to DNA
responsible for translation.of.the gene?
a) Excision of an intron 50. Which of the following are found only in
organisms containing poiycistronic mRNA?
b) mRNA without any secondary structure
c) Ribosome lacking ssRNA a) Alternative splice sites
d) A suppressor tRNA b) Temperature sensitive mutations
c) Polar mutations
44.Who of the following carried out d) Missense mutations
recombination experiments on rll locus of
phage T4 and Introduced the term 'Cistron'? 51. Frederick Griffith injected mice with samples
a) Seymour Benzer from virulent S strain andlor nonviruient R
b) Sydney Brenner strain of Streptococcus pneumoniae. Which
c) Daniel Nathans of the following o b ~ e ~ a t i 0 nis~ not
d) Paul Berg consistent with his experiment?
a) Mouse lived uDon inlection with R strain
45. The recognition site of the restriction b j Mouse lived upon iijection with a mixture of
endonuclease Avat is CPyCGPuG, where Py heat killed Strain and live R strain
is any pyrimidine and Pu is any purine. c) Mouse died upon injection with S strain
Which of the following would be the expected d) Mouse lived upon injection with heat killed S
average distance in nucleotide pairs between strain
Avel specific site in a random DNA
sequence? 52. Transfer of F' (F prime) factor to F- cells is
a) 64 known a6
b) 512 a) Conjugation
c) 1024 b) Transduction
d) 4096 c) Sexduction
d) Transformation
46. A mutation in a bacterial aminoacyi tRNA
synthetase led to charging of entire ~RNA'*' 53. Which of the following products of genetlc
population w ~ t h Alanine. Which of the manipulation will not qualify to be called a
following will occur upon using these transgenio?
aminoacyl tRNAs for protein synthes~sin a a) Obtained by micro-injection of DNA
bacterial cell? b) Obtained via direct DNA uptake by
a) Prote~n synthesized will contain either protoplasts
Alanine or serine c) Derived by somatic hybridization between
b) Protein synthesized will contaln Alanine cultivated and wild-related species
where serine should normally occur d) Gene transferred by backcross breeding with
c) Protein synthesized will contain serine where a transgenic
Alanine should normally occur ,
d) The a l a n y i - t ~ ~ ~ S e rnot
w i function
ll in protein
synthesis
Subject : PbD - Molecular Biology and Biatechnology - 2011 5

54. A circular double stranded DNA genome is
determined bv chemical method to contain
22 percent ' ~ u a n o s ~ n enucleotide The
percentage of adenosine nucleotide in the
DNA is
a) 11
b) 22
c j 28
d) Cannot be computed with the available
information
55. Which of the following conditions is likely to
interfere with the conjugation process in
bacteria?
a) Pretreatment of recipient cells with
cycioheximide
b) Pretreatment of recipient cells with DNase
c) Treatment of mating cell pairs with RNase
d) Application of strong shearing forces on the
mating cell pairs
56. A yeast artificial chromosome contains ail
these except
a) Centromere
b) Teiomeres
c) Satellite DNA
d) ARS
57. Which of the following does not contain
introns?
a) 5s and 5.8s rRNA , d) Methionlne and tryptophan
b) Genes of mitochondrial genome
c) Genes of nuclease genome
d) Genes of chloroplast genome
58. The cytoplasmic substrate for fatty acid
synthesis, acetyl CoA is synthesized in
1 60. Which of the following is the first metabolic
I ~roductwhich is common to the aerobic
metabolism of fatty acids and glucose?
a) Glyceraldehyde bphosphate
b) Pyruvate
c) Acetyl CoA
d) Citrate
61, The glyoxylate cycle is present in plants and
bacteria but is not found in animals. The lack
of glyoxylate cycle in animals results in their
~nabilityto
a) Perform giuconeogenesis from amino acids
b) Perform g~ucondogenesisfrom fatty acids
c) Synthesize oxaloacetate from isocitrate
d) Synthesize glutamate from malate
62. Attenuation. a
' /regu~atoryniechanism in trp
operon to overcome termination of
transcription occurs
a) When tryptophan concentration is iimitin'g
b) When tryptophan concentration is high
c) When c-AMP is low
d) When c-AMP is high
63.Which of the following amino acids are
specified by single codons?
a) Tryptophan and histidine
b) Methionine and proline
c) Methionine and histidine
64. The restriction enzymes that recognize a
specific target DNA sequence which is
I symmetrical and cleave within that sequence
are
-. - known
a) Type I
. . .- . .. . as
.

mitochondria. Which of the following is the b) Type 11 ,

form in which the carbon of acetyl CoA is c) Type ill
transported to the cytoplasm since the inner d) Type IV
mitochondriai membrane is impermeable to
acetyl CoA? 65. Which of the following can act as a substrate
a) Pyruvate for nitrogenase?
b) Citrate a) Nitrite
c) Acetate b) Ethylene
d) Malate c) Nitrate
d) Ammonia
59. In a ceii-free protein synthesizing system, a
syntQ?tic mRNA of the following sequence
66. The short single stranded 5' projections
~'CACACACACACACACAC~' was used.
which are complementary in sequence and
Assuming that protein synthesis could begin
w~thoutthe requirement of an initiator codon, by which A bacteriophage DNA adopts a
which of the following products will be circular structure when it is injected into its
synthesized? host cell are called
a) att site
a) One protein with an alternating sequence of b) int site
3 amino acids
c) cro site
b) One protein wlth an alternating sequence of
2 different amino acids d) cos site
c) One protein consisting of a single type of
amino acid
d) Two proteins each with an alternatlng
sequence of 2 different amino acids
 -
Subject : PhD- Molecular Biology alld Biotechnology 2011 6

67. The electron transport chain is located 74. The separation of two strands of duplex DNA
predominantly In which of the following in at the replication fork Is catalyzed by
mitochondria? a) Gyrase a

a) Inner membrane b) DNA helicase

b) Outer membrane
c) Inter membrane space
d) Matrix
68 Two formulations containing different growth
regulators are to be tested for tissue culture
response. Which of the following treatments
will be appropriate for a proper comparison?
a) Pairs of treatments with equal quantity (mgll)
of the samples
b) Palrs of treatments with equal quantity (mgll)
of active ingredients present in the sample
c) pairs of treatments with equimolar quant~tyof
active ingredients
d) Pairs of treatments with equal quantity (in%)
of active ingredients
89. The initial product of photosynthetic CO,
fixation in Cg plants is
a) Phosphoenoi pyruvate
b) Giyceraldehyde-3-phosphate
c) 3-phosphoglycerate
d) 1,3-bisphosphoglycerate
70. The fused hybridoma cells in monoclonal
antibody production are selected on a
medium containing wtiich of the following
a) Hypoxanthine, aminopterln, ampicillin
b) Hypoxanthine, thymidine, aminopterin
c) Hypoxanthine, ampiciliin, thymidine
d) Aminopterin, ampicillin, thymldine
71. Which of the following amino acids causes
disruption in helical structure in proteins?
a) Alanine
b) Valine
c) Proline
d) Tryptophan
72. The linkage number of a covalently closed
circular DNA of 21,000 bp which is free of
supercoiling and corresponds to B form of
DNA is
a) +zoo0
b) +ldD0
C) +2100
d) -2100
73. Which of the following is the com_mon lesions
found in DNA afler exposure to ultraviolet
light?
a) Base deletions
b) Purine dimers
c) Pyrimidine dimers
d) Single strand breaks
c) Topoisomerase
d) Single strand binding protein
75. The primary enzyme involved in replication
of E.'colichromosome is
a) DNA polymerase I
b) DNA pplymerase 11
c) DNA p81ymeraseI11
d) DNA polymerase 1V
76. Which of the following is the strategy
employgd by some viruses to increase the
coding pot6ntjal of their genome?
a) Use of a degenerate triplet code
b) Use of alternative splicing sites
c) Use of host ribosomes for translation
d) Integration Into the host genome
77. The percentage of G-C base pairs in a DNA
molecule is related to the Tm because
a) A-T base pairs require a higher temperature
for denaturation
b) The stability of G-C and A-T base pairs is
intrinsically d~fferent
c) The triple bonds o f G-C base pairs are less
stable than the double bonds of A-T base
pairs
d) The G-C content equals the A-T content
78 Which of the following statements is true for
an enzyme cataiysed reaction?
a) Competitive inhibition can be overcome by
increasing the concentration of substrate
b) Competitive inhibition can be overcome by
decreasing the concentration of substrate
c) Noncompetitive inhibition can be overcome
by increasing the concentration of substrate
d) Noncompetitive inhibition can be overcome
by decreasing the concentration of substrate
79. Cholesterol is a precursor for the synthesis
of all of the following except
a) Bile salt
b) Estrogen
c) Corticosteroids
d) Isoprene
80. During signal transduction, regulation of the
kinase cascades requires the activity of an
enzyme that
a) Removes phosphates
b) Cleaves proteins into peptides
c) Adds sugar groups to proteins
d) Makes disulfide bonds
 -
Subject :PhD Molecular Biology and Bioteclinology - 2011 7

81 The antibiot~cstreptomycin acts by 88. Commonly used software for multiple protein
a) Blocking protein elongation sequence alignment is
- b) Preventing initiation of protein synthesjs a) NCBl
b) CLUSTAL W
c) Interfering with peptidyl transferase activity
d) ,Interfering decoding by 16s rRNA, thus c) PHYLLiP
preventing protein synthesis - d) PAUP

82. Which of the following statement is not 89. Depending on the criteria such as quality and
correct? content of information, reproducibility arid
a) Prototrophs can grow on minimal media speed of different DNA marker systems,
b) Prototrophs can grow on complete media identify the most suitable arrangement inthe
c) Auxotrophs can grow on minimal media descending order
d) Auxotrophs can grow on supplement media -
a) AFLP - SSR RFLP - RAPD
-
b) RFLP AFLP - SSk - RAPD
83. Clues about the function of a plant gene -
c) SSR RFLP AFLP RAPD -
responsible for a disease can be obtained by d) RAPD-SSRrRFLP-AFLP
a) Carrying out the structural features of the
isolafed protein encoded by the gene 90. For construction 'of a linkage map, both
b) Analyzing the expression pattern of the gene AFLP and RAPD markers were used. Which
C) Expressing the gene in a bacterial system of the following mapping popuiation would be
d) Comparing the sequence of the putative most suitable and equally informative as that
gene causing the disease with sequences of of co-dominant markers for map
other genes stored in a database construction? . . .
a) F2of a cross between two inbred parents
84. An error has been made in the concentration b) F1 of a cross between two out-bred parents
of ddGTP used in dideoxy sequencing c) DH and RlLS derived from a cross between
reactions: the solution is 100 pM rather than two inbred parents
1 pM. Examination of the autoradiogram d) None of the above
resulting from using this concentration of
compound would show 91. During the recurrent backcrossing, the
a) increased spacing between all bands popuiation of the donor genome is reduced
b) Decreased spacing between all bands bv the relationshi~
c) A large blank area at the bottom
d) A decreased number of bands in the lane
from the reaction w ~ t hddGTP

85. Which of the following is not true for where m is the number of generation of
allosteric proteins? backcrossing
a) They obey Michaelis-Menten kinetics
b) They show sigmoidal relationship between 92.In human, the haploid number of
reaction velocity and substrate concentration chromosomes is 23. How many different
c) Activity of these enzymes may be altered by types of gametes can arise by independent
regulatory molecules which is bound to sites assortment? -
other than the catalytic sites a) 223
d) Binding of oxygen to hemoglobin is an
example of allosteric protein

86. Which ehistone is not a part of nucleosome

core pdlticle? 93. Gene conversion refers to
a) H2A a) A DNA segment being reversed in a
chromosome
b) Insertion of a transposons that alters the
reading frame
c) Single nucleotlde substitution caused by a
87. Which of the following radioactive molecules base analogue mispairing
is used for end-labeling of oiigonucleotides? d) Alterations in homologues as mismatch palr
a) U ~ ~ P - A T P errors are corrected
b) U ~ ~ P - C T P
c) Y ~ ~ P - A T P
d) Y ~ ~ P - A T P
 -
SubJect : PltD - Molecular Biology and Biotechnology 2011 8

94. Okazaki fragments are used to elongate 101. Which one of the following is not involved in
a) The leading strand towards the replication regulating the synthesis of RNA in the
fork eukaryotic nucleus?
b) The lagging strand towards the replication a) Active genes in euchromatin and genes in
fork heterochromatin
c)' The leading strand away from the replication b) Use of different RNA polymerases to
fork transcribe different classes of RNA
d) The lagging strand away from the replication c) Spliceosomes that stimulates synthesis of
fork intron-containinghnRNA
d) Enhancers that can stimulate specific
95. Sickle cell anaemia is due to a mutant gene, promoters
Hbs. The nature of this mutation is
a) Frameshift 102.How can yoq distinguish a solution
b) Transversion containing RNA from the one containing
c) Transition DNA?
d) Translocation a) Heating the solution to 85'C and then
measuring the apsorbance at 260 nm
96. The' number of introns in cDNA having 7 b) Measuring th6 absorbance at room
exons are temperature at 260 nm
a) 0 C) Measuring the absorbance at 280 nm at
b) 6 37OC
-. -
c) 7 d) Monitoring the change in absorbance at 260
d) 8 nm while elevating the temperature

97. Two protoplasts from distantly related plant 103. Which of the following chemicals is added In
species can be fused by the agarose gels during RNA
a) Polyethylene glycol electrophoresis?
b) Spermidlne a) SDS
c) Cellulase b) Formaldehyde
d) Pectinase c) Bromophenol blue
d) Urea
98.Maie sterllity systems are used for
commercial hybrid seed production in plants. 104. PEP carboxylase is the main enzyme to fix
In a plant species where seed is not the C02 in
economic product, most suitable male a) C3 plants
sterllity system for hybrid seed production b) C4plants
could be c) CAM plants
a) Recessive nuclear male sterility d) Both C3 and Cq plants
b) Cytoplasmic male sterility
c) Cytoplasmic genetic male sterility 105.Which of the following enzymes in
d) Dominant nuclear male sterility prokaryotes has a function opposite to that
of topoisomerase?
99. Which one of the following anonymous DNA a) Helicase
marker systems could be regarded as sing!? b) Gyrase
locus, multi-allelic and co-dominant marker? c) RNA polymerase
a) RAPD . d) Recombinase
b) SNP
c) SSR , 106.Who proposed chemiosmotic theory
d) AFLP of ATP generation during oxidative
phosphorylation?
100. Which one of the foilowlna Is not a feature a) Peter Mitchell
of eukaryotic gene expression?
a) Poiycistronlc mRNA are very rare
b) Many genes are interrupted by non-coding
/ bj Roved Emmerson
c) Albert Lehninger
d) James Summer
DNA sequences
c) RNA synthesis and protein synthesis are 107. The eukaryotic RNA polymerase I, II and IiI
coupled as in prokaryotes have been so named based on
d) mRNA is often extensively modified before a) Genes transcribed by them
translatlon b) Order of elution from a column
c) Order of their discovery
d) An arbitrary nomenclature
 Subject : PIID - Molecul~rBiology and Bioteel~nology- 2011 9

108. Predlct~onof conformation of a protein can 116.Which of the following enzymes can
be attempted from its introduce negative supercoillng?
a) Molecular weight a) Helicase,
b) Amino acid sequence b) Micrococcal DNase
, c) Number of amino acids c) DNA gyrase
d) lsoelectric pH d) Exonuclease Ill

109. The term 'BLAST' is an abbreviation for 117. Histamines are released from
a) Biological Linkage Analysis Statistical Tool a) B-cells
b) Basic Local Alignment Search Tool b) T-cells
c) Basic Localized Arrangement Tool c) Mast cells
d) Best Local Arrangement of Sequence Tags d) Phagocytes
110. One dalton is equal to 118. 100 p1 of 160 mM stock of each dNTP was
a) Io - gm~ mixed to constitute a master stock For a
b) 3.32 x gm PCR reaction requiring 200 pM of each
c) , 10.'~gm dNTP in'a 75 pI reaction volume, how much
d) 3.32 x 10''~gm of the master stock is required?
a) 1
111. Starch is a polymer of glucose with linkages b) 0.1 111
of c) 0.2 p1
a) a(1-+6) and p(l+4) d) 0.5 pl
b) a(1+4) and p(1-6)
c) a ( l j 6 ) and a(l+4) 119. Lysogeny of E. coli cells by A phage 1s
d) P(1-4) and p(1+6) maintained by continuous expression of the
following h gene
112. A gene is 10 map units away from each of a) N gene
the two morphological markers flanking the b) Cro gene
gene on either side. I n a test cross progeny, c) C~gene
what is the probability that a segregant d) gamgenes
carrying the target gene cannot be correctly
jdentified by the linked markers? 120.In an insertional cloning vector for
a ) 0.10 identifying clones by immunologicai
b) Zero methods, a cDNA is inserted into EcoRl site
C) 0.001 of the vector, what Is the probability that the
d) 0.01 ligation would lead toan in-frame fusion?
a) 116
113.The half-life of 3 2 ~ phosphate is b) 112
approximately 14 day;? 42 days after c) 113
purchase of a batch of P the radioactivity d) 114
present was 1.25 mCi. The radioactivity on
the date of purchase would be 121. if a virus particle contains double stranded
DNA of 120,000 base pairs, how, many
phosphorus atoms would be present

114. The number of linkage groups found in an

brganism is equal to the number of
chromosomes in its 122. p-oxidation of fatty acid yields
a) Haploid a) Glucose
b) Diploid b) Acetyi CoA
c) Triploid c) Glycerol
d) Tetraploid d) Triglyceride
115. If there are 10 DNA molecules at start, how 123, To isolate plant DNA, EDTA is used for
many DNA molecules will be present after a) Chelating cesium chloride
five cycles of replication? b) Inhibiting nuclease activities
a) 105 c) Removing ethidium bromide
b) 1 0 ~ 2 ~ d) Removing excessive NaCl
c) 10x5'
d) 2 ~ 1 0 ~
 Subject : PhD -Molecular Biology and Biotechnology - 2011 10

124 The relatiohship among linking number (Lk), Matchlng type questions (No. 131 to 140);
Twist (Tw) and Writhe (Wr) for a DNA all questions carry equal marks. Choose
molecule can be represented as the correct answer (a, b, c, d or e) for
a) L k + T w = W r
b) L k + W r = T w each sub-question (i,ii, iii, iv and v) and
c) T w + W r = L k enter your choice in the circle (by
d) T w - W r = L k shading with a pencil) on the OMR -
answer sheet as per the instructions
125. Whlch of the following functions is not given on the answer sheet.
shared between E. coli DNA polymerase I
and DNA polymerase ill? 131. Match the name of the scientists with their
a) 5 ' 3 DNA synthesis discoveries
b) Proof-reading i) Charles Yanofpky a) Colony hybridizatio n
c) 5'3 exonuclease activity ii) Grunstein and Hogness b). Hvbridoma
.
d) 3'4' exonuclease activity technology
iii) Beadle and Tatum c\ A ttenuation
126, What will be the maximum number of i;) ~ o h l e and
r ,Milstein
., dj ~estrictionenzymes
changes necessary to convert a seven v) Warner Arber, Nathans e) One Gene One
nueleotide-long DNA sequence into a and Smith Enzyme hypothesis III
palindrome?
a) 2 132. Match the following antibiotics with their
b) 3 mode of action
c) 4 i) Tetracycline a) Prevents release of EF-G-
d) 5 GDP from the ribosome
ii) Puromycin b) Inhibits amino-acyl tRNA
127. Which of the following tests is not based on binding to A site
antibodies? iii) Fusidic acid c) Blocks exit of growing
a) Western blott~ng polypeptide chain frotn
b) ELISA-test ribosome and assets
C) Northern blotting translation
d) Bt-strip assay iv) Erythromycin d) lnhibits peptidyi transferase
activity in eukaryotic cells
128. Cot%of DNA is defined as v, Cycloheximide e) Mimics the 3' end of
a) Time taken by the denatured DNA molecule aa-tRNA in A site anci
to reanneai causes chain termination
b) Concentration of DNA in a cell at a
particular time 133. Match the following types of lipids with their
c) Initial concentration of DNA multiplied by function in higher plants
time taken for 50 per cent of DNA to i) Glycerolipids, a) Water proofing and surface
reanneal sphingolipids protection
d) Concentration of cytosine bases In a single ,ii) Triacylglycerol, b) Defense and antifeeding
stranded DNA molecule waxes compounds
iii) Tocopherols C) Membrane structural
129. The two technical developments that led to components . .
the discovery of path of carbon during C02 iv) Cutin, suberin d) Protection of membranes
fixation in CQcycle against damage from free
a) Autoradiography and gel electrophoresis radicals
b) A~$oradiographyand paper v) Essential oils e) Storage compounds '
chromato~lrauhv
c) ultra-cent;ifugailon and autoradiography 134.
d) X-ray crystallography and capillary Techniaug Application
electrophoresis i ) Chromosome walking a) Secondary metabolite
production.
130. From the following recognition sequences of ii) Hairy root culture , b) Screening genomic
restriction enzymes, which one upon libraries :-
digestion of DNA is exuected to uroduce the iii) Southern blotting c) Map based cionlngl
3' recessed and 5' overhang termini iv) 2D-electro~horesis d) Gene transfer
V) ~lectropoiation e j Proteomics
a) GLGATCC
 Subject :PhD - Molecular Biology and Biotechnology - 2011 I1

138.
Transaenic orqanism ADDliCatiOn i) A.Z. Fire a) Caenorhabditis elegans.
i) Transgenic pig a) Rapeseed development '
i ~Golden
) rice b) Milk with therapeutic ii) S. Brenner b) Recombination in bacteria:
!
iii) Flavr Savr
proteins
c) Organ transplantation
iii) J. Lederbera -
c). Structure
,
proteins
-
of alobular

iv) Transgenic cow d) Delayed ripening iv) H.G. Khorana d) RNA interference
v) Barnase-barstar -
e) Vitamin A rich, . . v) M.F. Perutz e) t-RNA synthesis

136. 139.
i) DNA methylation a) Insertional mutagenesis i) Gyrase a) 0kazakl fragment
ii) Reverse genetics b) Selection marker ii) dsDNA b) SSb
' . iii) BAC C) Epigenetic mechanism iii) RNA primer , c) Anti-parallel
: iv) Antibiotic resistance d) Gene knockout . . iv) ssDNA d) dnaB
v) AoDs system

137.
e) Positional cloning V) Hellcase

140.
:. e) Topoisomerase

i) Thiamine a) Hormone i) Pyruvate kinas; a) Peroxisomes

ii) Tyrosine b) Base !, ii) Cataiase b) Glyoxysomes
lii) Renln c) Protease iii) Pyruvate carboxyiase c) Antiporter
: lv) Thymine d) Vitamin iv) Malate synthase d) Glycoiysis
V) Insulin e) Amino acid v) ATP-ADP translocase e) Gluconeogenesis
Subject : PhD- Molecular Biology and Biotechnology - 2011

Short questions (No. 141 to 146); each question carries FIVE marks. Write answers,
including computation I mathematical calculations if any, in the space provided for
each question on the question paper itself.
141. Given below is the Southern hybridization pattern obtained using DNA from leaves and flower buds
of a plant after restriction digestion with Hpall or Mspl. The probe used for hybridization is a cDNA
clone. Interpret the results.
HpaIl Mspl
Leaf Flower bud Leaf Flower bud

142. List the methods used for cloning a gene which is known only by its phenotype.
Subject : PhD -Molecular Biology and Biotechnology -2011 13

143. List atleast six possible modifications thatcan

. . . in a prokaryotic gene so that its
be carried out
.-.-z
expression in transgenic plant is enhanced.

144. List atleast six advantages of using plants as bioreactors for pharmaceutically relevant proteins
/.
 Subject : PhD -Molecular Biology and Biotechnoiogy - 2011

145. How can onesdeterminethe copy nu* atatcansgwein

,. a transgenic alant?
" ,
' P 6

146. Describe in brief the principle underlying 454. method of DNA sequencing.