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Determination of Sucrose
(by polarimetry)
INTRODUCTION
OFFICIAL RECOMMENDATIONS
DIVISORS
Mix well and filter, again rejecting the first 25 ml of filtrate. Pipette one
50-ml portion of the lead-free filtrate into a 100-ml flask, dilute with water
to the mark, mix well and polarize in a 200-mm tube. The result, multi-
plied by 2 is the direct reading, P, of the formula given below. (If a
400-mm tube is used the reading equals P.) If there is a possibility of
mutarotation, allow sufficient time for its completion.
(ii) Invert reading. - First determine the quantity of acetic acid necessary
to render 50 ml of the lead-free filtrate distinctly acid to methyl red
indicator. To another 50-ml portion in a 100-ml volumetric flask, add
the requisite quantity of acid and 5 ml of invertase solution, fill the flask
with water nearly to 100 ml. Stand overnight (preferably at a temperature
10 D E T E R M I N A T I O N OF S U C R O S E
not less than 20 C), dilute to volume at 20 C, mix well and polarize at
20 C in a 200-mm tube. Correct the polarization for the optical activity
of the invertase solution and multiply by 2 to obtain the invert reading
for a normal solution (I).
Determine total solids by refractometer and multiply this figure by
the density at 20 C to obtain total solids from original solution in 100 ml
of the invert solution (g).
If a rapid inversion is required, use 10 ml of invertase solution and
stand in a water bath at 55-60 C for 15 min with occasional shaking.
Cool, add sodium carbonate until distinctly alkaline to litmus paper,
adjust to volume and polarize as described above. Allow the solution
to remain in the tube and again determine the polarization. If there is
no change from the previous reading, the mutarotation is complete.
If it is necessary to work at a temperature other than 20 C, both
polarizations should be made at the same temperature, which must be
as close as possible to 20 C.
Calculate the percentage sucrose, S, in the original sample from
and allow to remain 30.8 h at 20C, 14.6 h at 25C or 7.1 h at 30C. Cool.
Make to volume and polarize at the same temperature as used for
direct polarization. Correct the rotation to the normal weight of sample (P ).
r
a. Double-enzyme Method
(see chapter 3. Determination of Raffinose)
where 7 V = / ' I + K,