1.

Expectation on this program

1) Personal Goal: Describe what you intend to achieve in this program.
Valuable experience can study in japan because it is one of my dream. learning
and sharing both of cultures we can communicate and understanding by each
other. Gaining knowledge about their high technology for research. Learning
Positive things such as discipline, hardworking and willing to learn in there and
reapplying it in my country so that Indonesia becomes better and more
developed. And my favorite thing is I can learn more about their language in
japan because I like japanesse language. With those explanations above, I am
sure that I am suitable to be candidate for PARE Program. I hope for the
admission committee can cosider my application. Iam really exited to be the
part of the program.

2. About your study

Thesis title (tentative) :Antioxidant activity effect of bioactive
peptide from enzimatic hydrolysate of
Thunnus sp. by-product

Brief Explanation about your thesis:

Human body needs oxygen to stay alive. However, oxygen is also
potentially damage body cells due to the oxidation process. The
oxidation process will release free radicals that have damage effects
on cells. According to the Umayyad and Amron (2007) free radicals
are molecules with one or more unpaired electrons that cause free
radicals become very reactive to find a partner by binding electrons in
a molecule that is around. Antioxidants are substance that interact
with and neutralize free radicals, thus preventing them from causing
damage. Synthetic antioxidants such as Butylated Hydroxyanisole
(BHA) and Butylated Hydroxy toluena (BHT) have stronger antioxidant
activity than natural antioxidant such as -tocopherol and ascorbic
acid, but its use has begun to be restricted because of the chemical
components in synthetic antioxidants capable of inducing DNA
damage and cause toxicity (Ito et al. 1986). Therefore, there is a
growing interest to identify antioxidative properties in many natural
sources including some dietary protein compounds. Fish and its by-
products represents as a rich source of nutrients of fundamental
importance for health diets such as protein and micronutrients
including vitamins, minerals and polyunsaturated fatty acid. Fish
protein hydrolysates have been identified as the rich source of
bioactive peptides with valuable pharmaceutical potentials. Recent
years, studies on bioactive peptides have received much attention in
worldwide because of their health benefits. According to Bougatef et
al. (2010) peptides hydrolysate from Sardinella aurita can exhibit
antioxidant activity. Thunnus sp. and its by-product is the potential
raw material to be used as a peptide hydrolyzate that act as
antioxidants because it has a high protein content and is thought to
have natural antioxidant components. In addition, the availability of
by-product of Thunnus sp. in Indonesia is relatively abundant and
research that lead to the use of a protein hydrolyzate that are
antioxidants is still rarely done. Based on the description above, it is
necessary to study for observing antioxidant activity of protein
hydrolysates Thunnus sp. by-product. The objectives of this thesis is
obtaining and identifying hydrolyzate peptide Thunnus sp. are
hydrolyzed enzymatically wich has antioxidant properties. This
research was conducted in several stages, the characterization of raw
materials, the production of Thunnus sp. by-product protein
hydrolyzate, the determination of -amino nitrogen content of the
free, the degree of hydrolysis, antioxidant activity test, and
characterization of protein hydrolyzate containing the highest
antioxidant activity.