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G-Biosciences

Molecular
Biology
Handbook &
Selection Guide

c
G-Biosciences 1-800-628-7730 www.GBiosciences.com
Protein Estimation Assays
Lysis Buffers & Systems
Apoptosis Assays
Protein Fractionation Kits
Cytotoxicity Assays
Dialysis (Micro) System
SAM Methyltransferase Assays
Electrophoresis Clean-Up
Protease Assays
Concentration Systems
Phosphatase Assays
Contamination Removal
Peroxide Assay

Protease Inhibitor Cocktails Proteomic Grade Detergents


Individual Protease Inhibitors Research Grade Detergents
Protease Assays Non-Ionic, Ionic & Zwitterionic
Proteases for Mass Spec. Detergent Estimations
Sequencing Grade Proteases Detergent Removal Systems

1-Hour Western System


Gel Preparation Chemicals Transfer Buffers & Membranes
Protein Marker Ladders Membrane Stains
Electrophoresis Buffers Blocking Buffers
Reducing & Alkylating Reagents Secondary Antibodies
Protein Gel Stains Detection Reagents
Reprobing Reagents

Protein Sample Preparation Affinity Resins


Protein Clean-Up Systems 6X His Protein Purification Kits
Electrophoresis Reagents GST Protein Purification Kits
Mass Spec Grade Protease Antibody Purification
InGel Digestion Kits Activated Resins
Peptide Generation Reagents Buffers & Reagents

Biotin Labeling
Carrier Proteins
Cell Surface Protein Labeling
Peptide Coupling Systems
Agarose Coupling Kits
Antibody Purification Resins
Fluorescent Dye Labeling Kits
Antibody Fragmentation Kits
Enzyme Labeling Systems

Coated Plates
Blocking Buffers Homobifunctional
Wash Buffers Heterobifunctional
Secondary Antibodies Optimizer Systems
Detection Reagents Cross-Linking Systems
Antibody Labeling Systems

Apoptosis Assays
DNA Isolation
Cytotoxicity Assays
Transformation & Screening
SAM Methyltransferase Assays
Polymerase Chain Reaction
Protease Assays
Agarose Electrophoresis
Phosphatase Assays
RNA Isolation
Peroxide Assay
Yeast Transformation
ELISA
Table Of Contents
Genomic DNA Purification 2 PCR 14
Introduction............................................................................2 DNA Polymerases...................................................................14
Purest DNA with OmniPrep. ................................................2 Taq Polymerase........................................................................14
OmniPrep . ..............................................................................2
Taq Polymerase (2X MasterMix).............................................14
OmniPrep for Tissue..............................................................2 Pfu Polymerase........................................................................14
OmniPrep for Blood................................................................3 Pfu Polymerase (2X MasterMix)..............................................14
OmniPrep for Plant.................................................................3 Deoxynucleotides...................................................................15
OmniPrep for Gram Positive Bacteria...................................3 Deoxynucleotides (dNTPs) Set................................................15
OmniPrep for Fungi................................................................3 Deoxynucleotides (dNTPs) Mix................................................15
OmniPrep for Yeast................................................................4 PCR Clean Up.........................................................................15
OmniPrep for Mouse Tail.......................................................4 Spin-OUT for PCR...................................................................15
OmniPrep Soil DNA................................................................4
Spin Format Isolation with GET...........................................4 RNA Purification 16
GET DNA Template.................................................................4
Tri-Xtract .................................................................................16

GET Plant DNA Template.......................................................4 Arrest Extraction Buffer.........................................................16


Protein Free DNA with XIT....................................................5 GET Total RNA........................................................................16
XIT DNA from Tissue..............................................................5 TOTAL Arrest ..........................................................................17
XIT DNA from Cells.................................................................5 RNase-DETECT ......................................................................17
XIT DNA from Blood................................................................5 RNaseOUT .............................................................................17
XIT DNA from Buccal Cells.....................................................6 RNasin......................................................................................17
XIT DNA from FFPE Tissue.....................................................6 Nucleic Acid Assay 18
XIT DNA from Mouse Tail.......................................................6
XIT DNA from Plant Tissues...................................................6 Nucleic Acid Electrophoresis 19
XIT DNA from Gram Positive Bacteria...................................7
Geno-ElectroPhore .................................................................19

XIT DNA from Gram Negative Bacteria.................................7
XIT DNA from Yeast................................................................7 Power Supplies.......................................................................19
XIT Mitochondrial DNA...........................................................7 GT 300 Power Pack...............................................................19
Mini-300 Power Supply...........................................................19
PCR Template DNA.................................................................8 G-CAPSULE.............................................................................20
OmniTemplate........................................................................8
GET AGAROSE DNA................................................................20
OmniPrep Soil DNA................................................................8
geneEXIT. ...............................................................................20
Rapid DNA Template Prep.....................................................8
Glow Dyes ..............................................................................21
Yeast Geno-DNA-Template. ...................................................8
Plant Geno-DNA-Template.....................................................9 DNA Ladders..........................................................................22
DNAmark 1kb Plus DNA Ladder............................................22
High Molecular Weight DNA..................................................9 DNAmark 500bp DNA Ladder...............................................22
MegaLong. .............................................................................9
DNAmark 100bp Plus Ladder...............................................22
Plasmid DNA Tools 10 DNAmark 100bp Ladder.......................................................22
DNAmark 50bp Plus Ladder.................................................22
Plasmid Isolation....................................................................10 DNAmark 20bp Ladder..........................................................22
GET Plasmid DNA Miniprep...................................................10
Accessories, Buffers & Chemicals........................................23
Bacteria Transformation........................................................10 Electrophoresis Running Buffers............................................23
Z-Competent E. coli Transformation.....................................10 Agarose Powders.....................................................................23
Plasmid Screening Reagents..................................................10 LabSafe Nucleic Acid Stain...................................................23
Plasmid Screening.................................................................11 FASTsilver ..............................................................................23
Plasmid Screening ToothPick. ..............................................11
ToothPick-PCR........................................................................11 Hybridization Buffers 24
EKONO ....................................................................................24

DNA Cleanup & Concentration 12 HYB-LINK................................................................................24
GET CLEAN DNA.....................................................................12
pinkCLEANUP ........................................................................12 Yeast Research Tools 25
Genomic Tube-O-DIALYZER. ..................................................12 EZ Yeast Plasmid Prep...........................................................25
Spin-OUT for PCR...................................................................12 Fast Yeast Transformation. ...................................................25
Spin-OUT.................................................................................13 Yeast Related Buffers..............................................................25
GET AGAROSE DNA................................................................13 Yeast Geno-DNA-Template. ...................................................26
geneEXIT. ...............................................................................13 LongLife Zymolyase............................................................26
LongLife Enzyme Preparations..............................................26
DNA OUT.................................................................................27
Molecular Biology Buffers & Chemicals...............................27
Molecular Biology Universal Kit..............................................27
Research Grade Water...........................................................27
Endotoxin Free Water..............................................................27
Molecular Grade Water...........................................................27
DEPC-Treated Water.................................................................27
Proteomic Grade Water...........................................................27
Buffers & Reagents.................................................................28
Updated: February 11, 2014
For further details, visit GBiosciences.com 1
Genomic DNA Purification
Introduction FEATURES
High Yield, ~100kb genomic DNA
A260/A280 between 1.8 and 2.0
A selection of genomic isolation kits are offered that purify high
quality genomic DNA from a wide variety of sources and for a wide Adaptable for variety of samples, including plant, bacteria, fungi,
array of applications. mammalian tissues, cells and blood
OmniPrep genomic DNA kits are for ultra pure genomic DNA Simple two tube method, 20-40 minutes
that is suitable for all downstream applications. These kits are fully No toxic chemicals, no phenol, no hazardous waste
scalable for large genomic DNA isolations. The procedure is slightly Suitable for ~150 preparations depending on sample
more involved to ensure ultra pure DNA. APPLICATIONS
GET genomic DNA kits are spin column format kits for the rapid Extraction of pure genomic DNA from cells and tissues, plant,
isolation of genomic DNA from small sample sizes. fungi, bacteria, whole blood, and other samples
XIT DNA kits produce protein free, high quality DNA and use Extraction of high quality, 100kb genomic DNA
protein digestion and precipitation followed by genomic DNA CITED REFERENCES
purification. No chloroform or phenol extraction required. Staley, C.A. et al (2012) Gene. 496:118
OmniTemplate genomic DNA kits are for the rapid generation of Li, Z. et al (2010) Protein Expression and Purification. 72:113
PCR ready genomic templates. Li, Z. et al (2010) Biochem and Biophys Res Comm. 402:519
Li, Z. et al (2009) Protein Expression and Purification. 67:175
MegaLong isolates high molecular weight genomic DNA from a Lin-Cereghino, J. et al (2008) Yeast. 25:293
variety of samples, including animal tissues, cultured cells, whole Choi, Y. and Shim, W. (2008) Microbiology. 154:326
blood, bacterial and yeast. Choi, Y. et al (2008) Mycologia. 100:701
Whitaker, V. et al (2007) J. Amer. Soc. Hort. Sci. 132:534

Purest DNA with OmniPrep Lee, B et al (2005) Genome. 48:1104


Pliss, L. et al (2004) J. Neurochem. 91:1082
Jacobs-Helber, S. et al (2002) JBC. 277:4859
Li, X. et al (2002) Genome. 45:229

OmniPrep Villar, M. et al (2001) J. Bact. 183:55

Cat. No. Description Size


High quality genomic DNA from diverse sources 786-136 OmniPrep >150 Preps
Isolates high quality genomic DNA from many different species
and tissue types including animal, plant, bacteria, yeast, fungi, whole OmniPrep for Tissue
blood, and cells in culture. DNA can be isolated from samples high
in polysaccharides or other contaminants that are difficult to remove High quality genomic DNA from animal tissue
from the DNA preparations. OmniPrep for Tissue kit has been modified to specifically isolate
Based on rapid precipitation technique that uses unique high quality genomic DNA from tissue samples, including fresh,
precipitation reagents to isolate genomic DNA free from proteins and frozen, fixed or paraffin-embedded tissue. The kit isolates DNA from
RNA. Pure genomic DNA is isolated in 20-40 minutes, depending bodily fluids, including plasma, serum, amniotic fluid, semen and CSF.
on the tissue sample type used. The resulting genomic DNA is Also isolates high quality DNA from cultured cells, non-mammalian
visualized on an agarose gel as a large single band, demonstrating nucleated blood and gram-negative bacteria.
high quality and minimal shearing. The kit isolates high purity (A260/A280 ratios of 1.8 to 2) DNA
(~100kbp) and the yield is 0.5-10g/mg tissue, 25-50g/ml gram
negative bacteria culture and 0.1-40g/ml body fluid, dependent on
starting material and quantity. If used according to the protocols this
kit purifies DNA from a total of 2gm solid tissue, 1x109 cultured cells
and 1x1010 gram-negative bacteria.
The kit uses a rapid precipitation technique that uses unique
precipitation reagents to isolate genomic DNA free from proteins and
RNA. Pure genomic DNA is isolated in 20-40 minutes, depending on
the tissue type used. Suitable for:
Mammalian tissue (fresh or frozen)
Figure 1: Omniprep scheme. Cultured cells
Ethanol or formalin fixed tissue
Suitable for a wide range of tissues and samples, including: Paraffin embedded tissue
Mammalian tissues (fresh or frozen) Bodily fluids, i.e. plasma, sera, amniotic fluid, semen & CSF
Cultured cells Nucleated blood cells from bird, fish and frog
Paraffin embedded tissues Gram negative bacteria
Ethanol or formalin fixed tissues
Nucleated blood cells from bird, fish and frog FEATURES
Bacteria (Gram positive and negative) High Yield, ~100kb genomic DNA
Plant tissues (fresh or frozen, rich in polysaccharides) A260/A280 between 1.8 and 2.0
Mouse tail Simple two tube method, 20-40 minutes
Yeast No toxic chemicals, no phenol, no hazardous waste
Fungi APPLICATIONS
Blood Extraction of pure genomic DNA from mammalian tissues
Blood stained and bodily fluid stained material Suitable for fresh, fixed, frozen or paraffin embedded tissue
Soil
The OmniPrep kit contains individual protocols for different Cat. No. Description Size
types of samples and instructions on how to adapt for large sample 786-395 OmniPrep for Tissue For ~2gm tissue
volumes.

2 For further details, visit GBiosciences.com


Genomic DNA Purification
OmniPrep for Blood OmniPrep for Gram Positive

High quality genomic DNA from blood Bacteria


OmniPrep for Blood kit isolates high quality genomic DNA from

High quality genomic DNA from bacteria
blood samples, including whole blood, buffy coats, packed cells and
bone marrow. OmniPrep for Blood isolates high purity (A260/A280 OmniPrep for Gram Positive Bacteria kit isolates high quality
ratios of 1.8 to 2.0) DNA (~100kbp) and the yield is between 5-30g/ genomic DNA from Gram-positive bacteria. The kit isolates high
ml, dependent on starting material and quantity. This kit is suitable purity (A260/A280 ratios of 1.8 to 2.0) DNA (~100kbp) and the yield is
for processing up to 100ml blood. 25-50g/ml Gram-positive culture. If used according to the protocols
The kit uses a rapid precipitation technique that uses unique this kit purifies DNA from 100ml Gram-positive bacteria culture.
precipitation reagents to isolate genomic DNA free from proteins and The kit uses a rapid precipitation technique that uses unique
RNA. Pure genomic DNA is isolated in 20-40 minutes, depending on precipitation reagents to isolate genomic DNA free from proteins and
the sample type used. OmniPrep for Blood isolates genomic DNA RNA. This kit is supplied with additional LongLife Lysozyme for rapid
from: digestion of bacteria cell walls and DNA release. Pure genomic DNA
Whole blood is isolated in 20-40 minutes, depending on the bacteria cell type
Buffy Coats used.
Packed cells FEATURES
Bone marrow High Yield, ~100kb genomic DNA
FEATURES A260/A280 between 1.8 and 2.0
High Yield, ~100kb genomic DNA Simple two tube method, 20-40 minutes
A260/A280 between 1.8 and 2.0 Supplied with LongLife Lysozyme for rapid bacterial cell wall
Simple two tube method, 20-40 minutes digestion
No toxic chemicals, no phenol, no hazardous waste No toxic chemicals, no phenol, no hazardous waste
APPLICATIONS APPLICATIONS
Extraction of pure genomic DNA from blood Extraction of pure genomic DNA from Gram negative bacteria
Suitable for whole blood, buffy coats, packed cells and bone Cat. No. Description Size
marrow 786-398 OmniPrep for Gram Positive Bacteria For 100ml culture
Extraction of high quality, 100kb genomic DNA
Cat. No. Description Size OmniPrep for Fungi
786-396 OmniPrep for Blood For 100ml blood
High quality genomic DNA from fungal tissue
OmniPrep for Plant
OmniPrep for Fungi kit isolates high quality genomic DNA from
fungal samples. The kit isolates high purity (A260/A280 ratios of 1.8 to
High quality genomic DNA from plant 2.0) DNA (~100kbp) and the yield is 0.2-1g/5mg fungal samples.
OmniPrep for Plant kit isolates high quality genomic DNA from If used according to the protocols this kit purifies DNA from 1-2gm
plant samples, including fresh and frozen plant tissues. The kit fungal tissues.
isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA (~100kbp) Based on a rapid precipitation technique that uses unique
and the yield is 0.5-3g/mg plant tissue. If used according to the precipitation reagents to isolate genomic DNA free from proteins and
protocols this kit purifies DNA from 20gm plant tissue. RNA.
The kit uses a rapid precipitation technique that uses unique This kit is supplied with Molecular Grinding Resin for the rapid
precipitation reagents to isolate genomic DNA free from proteins and release of DNA from the fungal tissue. Pure genomic DNA is isolated
RNA. Pure genomic DNA is isolated in ~20-40 minutes. in 20-40 minutes, depending on the tissue type used.
FEATURES FEATURES
High Yield, ~100kb genomic DNA High Yield, ~100kb genomic DNA
A260/A280 between 1.8 and 2.0 A260/A280 between 1.8 and 2.0
Simple two tube method, 20-40 minutes Simple two tube method, 20-40 minutes
Overcomes the high concentration of plant polysaccharides Molecular Grinding Resin for efficient fungal disruption
No toxic chemicals, no phenol, no hazardous waste No toxic chemicals, no phenol, no hazardous waste
APPLICATIONS APPLICATIONS
Extraction of pure genomic DNA from plant tissues Extraction of pure genomic DNA from fungal tissue
Suitable for fresh or frozen plant tissues
CITED REFERENCES
Cat. No. Description Size Lorch, J. et al (2010) J Vet Diagn Invest. 22:224
Szabo, L. J. (2007) Mol. Ecol. Notes. 7:92
786-397 OmniPrep for Plant For 20gm plant tissue Ordonez, M. E. and Kolmer, J. A. (2007) Phytopathology. 97:574
Sagaram, U. S. et al (2006) Mol. Plant Pathol. 7:381
Mertens, J. A. et al (2006) Arch. Microbiol. 186:41

Cat. No. Description Size


786-399 OmniPrep for Fungi For 2gm fungi

For further details, visit GBiosciences.com 3


Genomic DNA Purification
OmniPrep for Yeast Spin Format Isolation with GET

High quality genomic DNA from yeast
OmniPrep for Yeast kit isolates high quality genomic DNA from GET DNA Template
yeast. The kit isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA
(~100kbp) and the yield is 3-6g/ml yeast culture. If used according Spin column genomic DNA isolation
to the protocols this kit purifies DNA from 300ml yeast culture. GET DNA Template is a spin column format kit suitable for the
The kit uses a rapid precipitation technique that uses unique preparation of DNA templates from blood, cells, fungal and animal
precipitation reagents to isolate genomic DNA free from proteins and tissue samples. The method involves the solubilization of samples in
RNA. This kit is supplied with a proprietary Yeast Suspension Buffer the supplied Template Extraction Buffer, following removal of cellular
and LongLife Zymolyase for the rapid resuspension and digestion debris and proteins the DNA is selectively bound to a high efficiency
of the yeast cell walls for DNA release. Pure genomic DNA is isolated GET Spin Column. The pure, genomic DNA is washed and eluted
in 20-40 minutes, depending on the tissue type used. from the column in a small volume of an elution buffer. The isolated
FEATURES genomic DNA is suitable for PCR and other applications.
High Yield, ~100kb genomic DNA
A260/A280 between 1.8 and 2.0
Simple two tube method, 20-40 minutes
Supplied with Yeast Suspension Buffer and LongLife Zymolyase
for efficient DNA release
No toxic chemicals, no phenol, no hazardous waste
APPLICATIONS
Extraction of pure genomic DNA from yeast tissue
Extraction of high quality, 100kb genomic DNA
Figure 2: General scheme for GET DNA Template and GET Plant DNA.
Cat. No. Description Size
786-400 OmniPrep for Yeast For 300ml culture GET DNA Template purifies mammalian genomic DNA free from
impurities known to inhibit downstream applications. The high yield,
extracted DNA is normally >50kb in size. The yield is ~1-40g DNA
OmniPrep for Mouse Tail per preparation, depending on the source and quantity used. The kit
is supplied with reagents for extracting DNA from 50 or 100 preps.
High quality genomic DNA from mouse tail
FEATURES
OmniPrep for Mouse Tail kit isolates high quality genomic DNA Spin column format
from mouse tail samples. The kit isolates high purity (A260/A280 ratios For extraction of genomic DNA from blood, cells fungal and animal
of 1.8 to 2.0) DNA (~100kbp) and the yield is 70-80g/cm tail. If tissue
used according to the protocols this kit purifies DNA from 100-200cm Compatible with PCR and other downstream applications
mouse tail.
The kit uses a rapid precipitation technique that uses unique Cat. No. Description Size
precipitation reagents to isolate genomic DNA free from proteins 786-353 GET DNA Template 50 preps
and RNA. This kit is supplied with additional LongLife Proteinase K 786-354 GET DNA Template 100 preps
for the rapid release of DNA from the tough mouse tail tissue. Pure
genomic DNA is isolated in 20-40 minutes.
GET Plant DNA Template
FEATURES
High Yield, ~100kb genomic DNA Spin column genomic DNA isolation from plants
A260/A280 between 1.8 and 2.0 A spin column format kit suitable for the preparation of
Simple two tube method, 20-40 minutes DNA templates from plant samples. The method involves the
Supplied with extra LongLife Proteinase K for efficient DNA solubilization of plant tissue in the supplied Template Extraction
release Buffer, following removal of cellular debris and proteins the DNA is
No toxic chemicals, no phenol, no hazardous waste selectively bound to a high efficiency GET Spin Column. The pure,
APPLICATIONS genomic DNA is washed and eluted from the column in a small
Extraction of pure genomic DNA from mouse tail tissue volume of an elution buffer. The isolated genomic DNA is suitable for
Extraction of high quality, 100kb genomic DNA PCR and other applications.
Purifies plant genomic DNA free from plant impurities known to
Cat. No. Description Size inhibit downstream applications. The high yield, extracted DNA is
786-401 OmniPrep for Mouse Tail For 200cm tail

normally >50kb in size. The yield is ~30g DNA per preparation,
depending on the plant source and quantity used.
OmniPrep Soil DNA The kit is supplied with reagents for extracting DNA from 100 x
50-100mg plant tissue.
Extraction of PCR Template DNA from Soil FEATURES
For further detail, see the PCR Template DNA section. Spin column format
For extraction of genomic DNA from plant tissue
Cat. No. Description Size Compatible with PCR and other downstream applications
786-469 OmniPrep Soli DNA 50 x 100mg preps Cat. No. Description Size
786-355 GET Plant DNA Template 100 preps

4 For further details, visit GBiosciences.com


Genomic DNA Purification
Protein Free DNA with XIT XIT DNA from Cells

Genomic DNA isolation kits that allow you to produce protein free, The XIT DNA from Cells kit is designed for the isolation of
high quality DNA through the principle of cell lysis, protein digestion genomic DNA from cultured cells.
and precipitation, and finally DNA precipitation to isolate high quality The kit uses rapid & efficient nuclear lysis and complete
genomic DNA. High quality DNA can be isolated from sample types protein precipitation, followed by DNA precipitation. The yield is
including: approximately 5-10g/1-2x106 cells.
Animal tissues Cells Whole blood FEATURES
Bacteria Buccal cells Plant tissues
Isolate high quality genomic DNA
Mouse Tail Yeast FFPE tissue
Suitable for adherent or suspension cultured cells
The XIT kits procedure removes contaminants and enzyme
Yield 5-10g/1-2x106 cells
inhibitors, allowing the purified DNA to be ready for immediate use for
Suitable for archive quality DNA
all downstream analyses.
Cat. No. Description Size
XIT DNA from Tissue 786-303 XIT DNA from Cells For 5x107 cells
786-304 XIT DNA from Cells For 5x108 cells
The XIT DNA from Tissue kit is designed for the isolation of
genomic DNA from fresh, frozen or methanol/ acetone fixed tissues.
XIT DNA from Blood
The kit uses three main steps that are rapid & efficient cell lysis,
enzymatic protein digestion and complete protein precipitation,

Designed for the isolation of genomic DNA from blood, bone
followed by DNA precipitation. This yields high quality genomic DNA.
marrow and buffy coat.
The kit is supplied with three protocols for the isolation of:
The kit uses four main steps that are red blood cell lysis, rapid
1-10mg and 50-100mg fresh or frozen tissue
& efficient nuclear lysis, enzymatic protein digestion and complete
5-10mg fixed tissue
protein precipitation, followed by DNA precipitation. This yields high
Three sizes of kit are available for processing a total of 0.25, 2.5
quality genomic DNA.
and 25g of tissue. The purified DNA has a A260/A280 ratio between
Three sizes of kit are available for processing a total of 12.5, 125
1.7 and 1.9 and is up to 200kb in size. Yield is approximately 0.5-
or 250ml of blood. The purified DNA has a A260/A280 ratio between
10g/mg tissue.
1.7 and 1.9 and is up to 200kb in size. The yield is approximately
10-15g/ml of blood.

Figure 3: XIT DNA from Tissue scheme.

FEATURES
Isolate high quality genomic DNA
Suitable for fresh, frozen and fixed tissue Figure 5: XIT DNA from Blood scheme.
Yield 0.5-10g/mg tissue FEATURES
Suitable for archive quality DNA
Isolate high quality genomic DNA
Suitable for blood, bone marrow and buffy coat
Yield 10-15g/ml blood
Suitable for archive quality DNA

Figure 4: 10mg mouse liver was treated with the XIT Genomic DNA
from Tissue kit. The precipitated DNA was hydrated in 30l TE buffer and
3l was loaded onto an agarose gel.
Figure 6: Genomic DNA was isolated from 500l bloodusing the XIT
Cat. No. Description Size Genomic DNA from Blood Kit.
786-345 XIT DNA from Tissue For 250mg tissue
786-346 XIT DNA from Tissue For 2.5g tissue Cat. No. Description Size
786-347 XIT DNA from Tissue For 10g tissue 786-294 XIT DNA from Blood For 12.5ml blood
786-295 XIT DNA from Blood For 125ml blood
786-296 XIT DNA from Blood For 250ml blood

For further details, visit GBiosciences.com 5


Genomic DNA Purification
XIT DNA from Buccal Cells XIT DNA from Mouse Tail

Designed for the isolation of genomic DNA from Buccal cells The XIT DNA from Mouse Tail kit is designed for the isolation of
(cheek cells). The kit uses three main steps that are rapid & genomic DNA from 5mm sections of mouse tail. The kit uses three
efficient cell lysis, enzymatic protein digestion and complete protein main steps rapid & efficient cell lysis with enzymatic protein digestion
precipitation, followed by DNA precipitation. This yields high quality and complete protein precipitation, followed by DNA precipitation.
genomic DNA. This yields high quality DNA.
The kit is available with cheek cell collection brushes or a The kit is available for processing a total of 125mm of mouse tail.
mouthwash method to collect Buccal cells. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up
The kits are available in two different sizes. The purified DNA has to 200kb in size.
a A260/A280 ratio between 1.8 and 2.0 and is up to 200kb in size. The
yield is approximately 3-5g/swab.

Figure 10: XIT DNA from Mouse Tail scheme.


Figure 7: XIT DNA from Buccal Cells scheme.
FEATURES
Figure 8: Cheek cells were collected with the supplied Isolate high quality genomic DNA
collection brush and the genomic DNA extracted with the
Suitable for archive quality DNA
XIT Genomic DNA from Buccal Cell kit. The isolated genomic
DNA was used as a template for PCR amplification. The Cat. No. Description Size
resulting PCR products are shown. 786-350 XIT DNA from Mouse Tail For 125mm mouse tail

Cat. No. Description Size


XIT DNA from Plant Tissues
786-341
786-342
XIT DNA from Buccal Cells (Mouthwash)
XIT DNA from Buccal Cells (Mouthwash)
For 25 samples
For 250 samples
Designed for the isolation of genomic DNA from fresh or frozen
786-343 XIT DNA from Buccal Cells (Collection Brush) For 25 samples
plant tissues. The kit uses three main steps that are rapid &
786-344 XIT DNA from Buccal Cells (Collection Brush) For 50 samples
efficient cell lysis, enzymatic protein digestion and complete protein
precipitation, followed by DNA precipitation. This yields high quality
XIT DNA from FFPE Tissue genomic DNA.
Two sizes of kit are available for processing a total of 2.5 and 25g
Designed for the isolation of genomic DNA from formalin fixed, of tissue. The purified DNA has a A260/A280 ratio between 1.7 and
paraffin embedded tissue. The kit uses three main steps that are 1.9 and is up to 200kb in size. The yield is around 1-5g/mg tissue,
rapid & efficient cell lysis, enzymatic protein digestion and complete dependent on plant species.
protein precipitation, followed by DNA precipitation. This yields high
quality genomic DNA.
The kit is for processing a total of 0.25g of tissue. The purified
DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in
size. The yield is approximately 0.5-10g/mg tissue.

Figure 11: XIT DNA from Plant scheme.

Figure 12: Genomic DNA was extracted from 100mg Arabidopsis


thaliana with the XIT Genomic DNA from Plant Tissue kit. The DNA
was rehydrated in 50l & 10l was loaded onto an agarose gel.

FEATURES
Figure 9: XIT DNA from FFPE Tissue scheme. Yield 1-5g/mg tissue
Suitable for archive quality DNA
FEATURES
Suitable for fresh, frozen and fixed tissue
Yield 0.5-10g/mg tissue
Suitable for archive quality DNA Cat. No. Description Size
786-297 XIT DNA from Plant Tissue For 2.5g tissue
Cat. No. Description Size
786-298 XIT DNA from Plant Tissue For 25g tissue
786-290 XIT DNA from FFPE Tissue For 250mg tissue

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Genomic DNA Purification
XIT DNA from Gram Positive Bacteria XIT DNA from Yeast

Designed for the isolation of genomic DNA from overnight Designed for the isolation of genomic DNA from overnight yeast
cultures. The kit uses three main steps that are rapid & efficient cultures.
enzymatic digestion of cell walls, spheroplast lysis and complete The kit uses three main steps that are rapid & efficient enzymatic
protein precipitation, followed by DNA precipitation. This yields high digestion of cell walls, spheroplast lysis and complete protein
quality genomic DNA. precipitation, followed by DNA precipitation. This yields high quality
Two sizes of the kit are available for processing a total of 25 genomic DNA.
and 250ml of bacterial culture. The purified DNA has a A260/A280 Two sizes of kit are available for processing a total of 25 and
ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is 250ml of yeast culture. XIT DNA from Yeast Kit protocol is designed
approximately 15-25g/ml culture. to use 1ml overnight culture, however the protocol can be easily
adapted for larger tissue sample sizes.The purified DNA has a A260/
A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield
is approximately 1-6g/ml culture.

Figure 13: XIT DNA from Gram Positive Bacteria scheme.

FEATURES
Yield 15-25g/ml bacterial culture Figure 15: XIT DNA from Yeast scheme.
Suitable for archive quality DNA
Cat. No. Description Size
786-339 XIT DNA from Gram positive bacteria For 25ml culture
786-340 XIT DNA from Gram positive bacteria For 250ml culture

XIT DNA from Gram Negative Bacteria Figure 16: Genomic DNA was isolated from 1ml overnight yeast culture, using
XIT DNA from Yeast kit. The DNA was resuspended in 50l and 5l or 10l
Designed for the isolation of genomic DNA from overnight were resolved on an agarose gel.
cultures. The kit uses three main steps that are rapid & efficient
bacterial lysis, complete protein precipitation, followed by DNA FEATURES
precipitation. This yields high quality genomic DNA. Yield 1-6g/mg tissue
Two sizes of kit are available for processing a total of 25 and Suitable for archive quality DNA
250ml of bacterial culture. The protocol is designed to use 1ml Cat. No. Description Size
overnight culture, however the protocol can be easily adapted for 786-348 XIT DNA from Yeast For 25ml culture
larger culture sizes. The purified DNA has a A260/A280 ratio between 786-349 XIT DNA from Yeast For 250ml culture
1.7 and 1.9 and is up to 200kb in size. The yield is approximately
20-40g/ml culture.
XIT Mitochondrial DNA

The XIT Mitochondrial DNA kit combines our FOCUS
Mitochondria technology with our XIT DNA isolation technology in
a single kit. The kit uses our proprietary Subcell buffers to lyse cells
and animal tissue and remove cellular proteins and nuceli from
a highly enriched, intact and active mitochondrial fraction. The
mitochondria are then lyzed with the included lysis buffer.
The lysate is treated with our LongLife Proteinase K to degrade
Figure 14: XIT DNA from Gram Negative Bacteria scheme. the proteins, which are then precipitated and the mitochondrial DNA
is isolated with alcohol precipitation.
FEATURES The kits is designed for ~100 preps consisting of 20x106
Yield 20-40g/mg tissue mammalian cells/ prep. The kit protocol also contains protocols for
Suitable for archive quality DNA isolation of mitochondra from soft (i.e. brain & liver) and hard (i.e.
Cat. No. Description Size cardiac & skeletal muscle) tissue.
786-337 XIT DNA from Gram negative bacteria For 25ml culture FEATURES
786-338 XIT DNA from Gram negative bacteria For 250ml culture Isolate high quality mitochondrial DNA
Suitable for cultured cells, soft and hard animal tissue
Cat. No. Description Size
786-301 XIT Mitochondrial DNA 100 preps

For further details, visit GBiosciences.com 7


Genomic DNA Purification
PCR Template DNA Rapid DNA Template Prep

For small sample size with low DNA content
OmniTemplate This kit is suitable for the preparation of DNA templates from
blood, cells, animal tissues and plant samples. The method involves
Single tube preparation of PCR ready genomic DNA solubilization of a sample in Template Extraction Buffer, followed by
templates the selective binding of DNA to our proprietary pinkRESIN. Following
OmniTemplate is specifically designed for the rapid isolation of a washing step, the DNA template is eluted from the pinkRESIN. The
a DNA template for polymerase chain reaction (PCR) analysis from isolated template is suitable for PCR and other applications. One
mammalian tissue samples, blood and cell cultures. This single tube preparation is for 1-10mg animal tissue or 50-100mg plant tissue.
method produces a high concentration, ready-to-use supply of DNA
template for microanalysis, genotyping and a multitude of other
applications.
The method involves isolation of nuclei and their subsequent
digestion with LongLife Proteinase K in the same tube the samples
were collected in. The isolated nuclei are digested to release
template DNA in a buffer compatible with PCR and other downstream
applications. The OmniTemplate samples can be directly used in
PCR and other applications without further manipulations. One prep
is approximately 1-10mg tissue.

Figure 18: Rapid DNA Template Prep scheme.

FEATURES
For animal and plant samples
Compatible with PCR and other downstream applications
APPLICATIONS
For the rapid preparation of DNA template
Cat. No. Description Size
786-014 Rapid DNA Template Prep 50 preps
Figure 17: OmniTemplate produces high quality genomic DNA template.
786-015 Rapid DNA Template Prep 100 preps
5l (lanes 1, 3, 5) or 15l (lanes 2, 4, 6) PCR reactions were loaded
onto a 2% agarose gel. Template was purified from 104 (lanes 1-2), 106
(lanes 3-4) or 2.8x106 (lanes 5-6) NIH3T3 cells. Yeast Geno-DNA-Template
FEATURES

Extraction of high quality DNA from yeast
Single tube genomic DNA isolation
Compatible with PCR and other downstream applications This extraction kit isolates high quality genomic DNA from yeast.
Suitable for blood, cells, and animal tissue samples The kit is based on a two-step lysis of yeast cells, using LongLife
No toxic chemicals, no phenol, no hazardous waste Zymolyase and LongLife Proteinase K, followed by the removal
of proteins and other cellular impurities by precipitation and
APPLICATIONS
centrifugation. The clean, genomic DNA is collected by precipitation
Designed for the rapid preparation of DNA template for large and the high yield of extracted DNA has an average 100kb length and
throughput microanalysis has A260/280 1.8-2.0.
High recovery of ready-to-use DNA templates in a short period of For extracting DNA from 100 x 1.5ml yeast cultures.
time
Cat. No. Description Size
786-013 OmniTemplate >100 preps

OmniPrep Soil DNA



Extraction of PCR Template DNA from Soil
The OmniPrep Soil DNA kit provides all the reagents necessary
to isolate PCR ready DNA for a large variety of environmental
samples. The kit is primarily designed for use with environmental
samples containing a high humic acid content, including difficult
soil samples such as compost, manure and sediment. A major Figure 19: Yeast Geno-DNA-Template scheme.
issue with high humic acid samples is the humic acids, and metals
FEATURES
and polysaccharides, inhibit subsequent PCR. OmniPrep Soil
Compatible with PCR and other downstream applications
DNA SoilOUT columns remove this interfering agents allowing for
successful PCR. APPLICATIONS
For the preparation of high yield, DNA template from yeast
Cat. No. Description Size
Cat. No. Description Size
786-469 OmniPrep Soli DNA 50 x 100mg preps

786-134 Yeast Geno-DNA-Template 100 preps

8 For further details, visit GBiosciences.com


Genomic DNA Purification
Plant Geno-DNA-Template High Molecular Weight DNA

Extraction of high quality DNA from plants
Plant-Geno-DNA-Template is suitable for the preparation of DNA MegaLong
templates from plant samples. The method involves the solubilization
of plant tissue in the supplied Template Extraction Buffer, following Extract high molecular weight genomic DNA with
removal of cellular debris and proteins the DNA is selectively bound minimal shearing
to pinkRESIN. The pure, genomic DNA is washed and eluted from The majority of genomic DNA extraction methods involve
the pinkRESIN in a small volume of an elution buffer. The isolated numerous physical manipulations, including mixing, pipetting,
genomic DNA is suitable for PCR and other applications. shaking, binding to resin, elution, which results in sheared DNA that
Plant Geno-DNA-Template purifies plant genomic DNA free from may not be suitable for further analysis.
plant impurities known to inhibit downstream applications. The high MegaLong isolates high molecular weight genomic DNA from a
yield, extracted DNA is normally >50kb in size. variety of samples, including animal tissues, cultured cells, whole
The kit is supplied with reagents for extracting DNA from 100 x blood, bacterial and yeast. MegaLong uses Genomic Tube-O-
50-100mg plant tissue. DIALYZER, a unique, patented micro dialysis device with a 0.45m
membrane that minimizes sample manipulation, one of the main
reasons for DNA breakage. Nuclei are isolated under mild extraction
conditions and genomic DNA is released by digestion of nuclear
proteins with a highly active LongLife Proteinase K. The digestion
is performed in the Tube-O-DIALYZER and after digestion the Tube-
O-DIALYZER is inverted to dialyze away digested protein and other
impurities leaving behind highly pure and fully hydrated genomic
DNA.
The fragile, high molecular weight genomic DNA can be stored in
the Tube-O-DIALYZER to further minimize mechanical manipulation
of the DNA. The DNA is suitable for Southern blot analysis, recovery
of Lambda shuttle vectors from transgenic animals, PCR, analysis by
pulsed-field electrophoresis or any application where genomic DNA is
Figure 20: Plant Geno-DNA-Template scheme. required.
FEATURES The kit is supplied in two sizes for the purification of either 25 or
50 x 1-100mg samples.
For extraction of genomic DNA from plant tissue
Compatible with PCR and other downstream applications
APPLICATIONS
Designed for the preparation of high yield, genomic DNA template
from plant
Cat. No. Description Size
786-135 Plant Geno-DNA-Template 100 preps

Figure 21: Megalong scheme.

FEATURES
Isolation of high molecular weight genomic DNA
For animal tissues, cultured cells, whole blood, bacterial and yeast
Uses patented genomic Tube-O-DIALYZER to minimize sample
manipulation
APPLICATIONS
For DNA libraries, PCR amplification, Southern blot analysis, pulse-
field electrophoresis, and other applications where >100kb DNA is
required
Cat. No. Description Size
786-146 MegaLong 25 preps
786-147 MegaLong 50 preps

For further details, visit GBiosciences.com 9


Plasmid DNA Tools
Plasmid Isolation Bacteria Transformation

GET Plasmid DNA Miniprep Z-Competent E. coli

Spin column plasmid isolation from 1-5ml culture; Transformation
single column or 96-well.
High efficiency competent cells in <20 minutes
Isolates high quality plasmid DNA from 1-5ml E. coli cultures.
Designed to generate competent E. coli cells for simple and highly
The kit utilizes an enhanced DNA binding column to produce high
efficient E. coli transformation.
yields of plasmid. This quick and easy protocol eliminates toxic
E. coli are grown in SOB medium, washed and suspended
phenol/chloroform extractions or lengthy ethanol precipitations. On
in the supplied Competent Buffer. The bacterial cells are now
completion of the protocol, the plasmid DNA is ready for restriction
ready for transformations. The transformation efficiency is 2x108-
enzyme digestion, sequencing, subcloning and in vitro transcription.
1x109 transformants per g of pUC19 plasmid. The efficiency of
Plasmid yields are typically up to 20g/prep. Available as single use
transformation varies on E. coli strain and plasmid size.
spin columns or a 96-well spin format.
The competent cells can be used immediately or stored for later
use. The kit is supplied with reagents sufficient for processing up to
300 x 100l aliquots of competent cells for transformations or with
reagents and SOB culture media for processing up to 100 x 100l
aliquots of competent cells.
FEATURES
No heat shock required
Competent cells in <20mins
Transformations in <5mins
APPLICATIONS
Generation of competent cells from E. coli
Rapid transformation of competent cells
CITED REFERENCES
Singh, S. et al (2005) Protein Sci. 14: 2095
Singh, S. et al (2005) Protein Sci. 14: 2601
Tyler, R. et al (2005) Proteins. 59: 633

Cat. No. Description Size


GZ-4 Z-Competent E.coli Transformation 300 preps
GZ-5 Z-Competent E.coli Transformation with SOB media 100 preps
GZ-5S SOB Media 50ml

Plasmid Screening Reagents



Antibiotics, IPTG, X-Gal and X-Gluc
HP Ampicillin is a stronger and more stable inhibitor of
Figure 22: GET Plasmid DNA single and 96-well plate general scheme. -lactamase than regular ampicillin and, therefore, supports only
the growth of ampicillin resistant colonies. HP Ampicillin is supplied
as ten lyophilized vials suitable for 500ml/vial. Regular ampicillin is
Figure 23: Comparison of GET Plasmid also offered.
Miniprep with Competitor Q. Overnight
IPTG induces activity of -galactosidase by binding and inhibiting
cultures were processed with G-Biosciences
GET or Competitor Qs Plasmid Miniprep the lac repressor. In cloning experiments, the lacZ gene is replaced
kit. DNA was eluted in an equal volume of with the gene of interest and IPTG is then used to induce gene
TE buffer and resolved. Both kits produced expression. IPTG is an effective inducer in the concentration range of
a strong band of supercoiled plasmid 100M to 1.5mM
DNA and some minor higher bands due X-gal is used to indicate whether bacteria express the
to nicked DNA, however Competitor Q had -galactosidase enzyme, which is encoded by the lacZ gene.
additional bands below the main band that X-Gluc is a substrate for -glucuronidase (GUS), which is encoded
are denatured plasmid DNA that is resistant
by gusA, a widely used reporter gene.
to restriction digestion.
CITED REFERENCES
FEATURES X-Gal
Isolate up to 20g ready-to-use plasmid DNA Oatley, M. et al (2011) Biol Reprod. 85:347
Spin or vacuum manifold compatible Cat. No. Description Size
No phenol, chloroform or alcohol precipitation GD-6060 HP Ampicillin [1000X] For 5L
50 and 100 prep kits available. RC-020 Ampicillin sodium salt 25g
Cat. No. Description Size RC-021 Ampicillin sodium salt 100g
786-361 GET Plasmid Miniprep 50 preps R036 IPTG (Molecular Biology Grade) 5g
786-362 GET Plasmid Miniprep 100 preps R035 X-Gal (Molecular Biology Grade) 1g
786-648 GET Plasmid DNA 96-well 4 x 96-well plates R037 X-Gluc (Molecular Biology Grade) 100mg

10 For further details, visit GBiosciences.com


Plasmid DNA Tools
Plasmid Screening

Plasmid Screening ToothPick

Rapid colony screening for plasmid DNA


Plasmid Screening ToothPick is a unique system that allows for
the rapid screening of bacteria for transformed plasmids, without the
need for an overnight culture.
Pick a colony, add to Plasmid Screening ToothPick reagents and
then analyze using restriction enzymes.

Figure 24: ToothPick general scheme.

FEATURES
Simple, 5 minute protocol, as easy as picking a colony
No overnight cultures required
APPLICATIONS
Rapid colony screening, using restriction digestion analysis
Cat. No. Description Size
786-026 Plasmid Screening ToothPick 300 preps

ToothPick-PCR

Rapid colony screening for plasmid DNA with PCR


ToothPick-PCR is an extension of ToothPick and allows for the
rapid release of plasmids from transformed bacteria for screening by
polymerase chain reaction (PCR). There is no requirement for growing
bacteria, performing minipreps or purifying the plasmid DNA.
Add your colony directly to the supplied ToothPick solution, heat
and then transfer 1l directly to a PCR reaction to screen for your
plasmid. Following PCR, add the supplied GLOW Loading Dye and
run on an agarose gel. No need to stain with ethidium bromide,
simply place gel on UV box and view.
Glow Dyes have been specifically formulated for nucleic acid
electrophoresis and contain an optimized concentration of buffer
agents, tracking dyes and ethidium bromide. These loading dyes
reduce exposure and many of the problems associated with ethidium
bromide use and disposal.

Figure 25: Screening of Plasmids with ToothPick-PCR. Four transformed


bacterial colonies were picked, resuspended in 15l ToothPick solution &
boiled for 5 minutes. 1l was added to a 50l standard PCR reaction & the
resulting PCR products were resolved on an agarose gel. Lanes 1 & 2 show
the correct plasmid.
FEATURES
Simple, 5 minute protocol, as easy as picking a colony
No overnight cultures required
Supplied with GLOW Loading Dye
Cat. No. Description Size
786-410 ToothPick-PCR 300 preps

For further details, visit GBiosciences.com 11


DNA Cleanup & Concentration
GET CLEAN DNA Genomic Tube-O-DIALYZER

For PCR clean up & removal of restriction enzymes. For the rapid clean-up of genomic DNA
GET CLEAN DNA uses our high binding affinity GET Spin

Genomic Tube-O-DIALYZER rapidly dialyzes >100kb genomic DNA
Columns to remove salts, enzymes, unincorporated nucleotides, samples to remove small DNA, salts, RNA and other contaminants
radiolabels, and primer-dimers from any DNA preparation of 100bp to with minimal hands-on manipulation.
>20kb. GET Spin Columns has an enhanced binding affinity for DNA, Based on our patented Tube-O-DIALYZER, the genomic Tube-
thus eliminating loss or damage of DNA. O-DIALYZER has a dialysis membrane secured in the cap. Simply
The DNA cleaning protocol takes as little as 5 minutes: pipette your >100kb genomic DNA sample into the Tube-O-DIALYZER
GET CLEAN DNA is available in a 50 or 100 prep size. tube, screw on the dialysis cap and dialyze. To recover 100% of
your sample, briefly centrifuge and replace the dialysis cap with the
supplied storage cap and store.

Figure 26: GET CLEAN DNA scheme.

FEATURES
Spin column format
Enhanced DNA binding affinity Figure 27: Genomic Tube-O-DIALYZER scheme.
Protocol is 5-10 minutes
No phenol/chloroform extraction or alcohol precipitations FEATURES
APPLICATIONS Rapid removal of impurities, RNA, small fragment DNA, etc.
PCR clean-up 100% sample recovery
Purifying DNA fragments after in-vitro labeling reactions Single tube for dialysis and storage, prevents sample loss
Removal of restriction enzymes from plasmid DNA For 0.2-2.5ml sample
Cleaning any DNA preparations from interfering agents No contamination
APPLICATIONS
Cat. No. Description Size
For the clean-up of genomic (>100kb) DNA by dialysis
786-356 GET CLEAN DNA 25 preps
Patented, single tube dialysis device to ensure no sample loss
786-357 GET CLEAN DNA 50 preps
Cat. No. Description Size

pinkCLEANUP 786-142-45MC Genomic Tube-O-DIALYZER 25



For the rapid clean up of DNA preparations Spin-OUT for PCR
Uses pinkRESIN to remove salts, enzymes, unincorporated
nucleotides, radiolabels, and primer-dimers from any DNA
Remove contaminants after PCR
preparations of 100bp to >20kb. pinkRESIN has an enhanced The SpinOUT PCR columns are spin format desalting columns
binding affinity for DNA and resuspends with ease, thus eliminating that have the ability to remove salts, radioisotpes, dyes, primers and
loss or damage of DNA; a major frustration of using binding matrix for deoxynucleotides (dNTPs) for nucleic acids following PCR. Two sizes
isolation of DNA. are available to remove contaminating products from PCR products,
The DNA cleaning protocol takes as little as 5 minutes. including <20bp primers, dNTPs and salts or from <32bp primers,
Optional spin columns are also offered to simplify the procedure dNTPs and salts.
further. PinkRESIN is also offered separately for those who prefer to Spin-OUT PCR is for the cleaning of PCR products. PCR-20
customize their own protocol. removes contaminating products from PCR products, including
FEATURES <20bp primers, dNTPs and salts. PCR-32 removes PCR products
from <32bp primers, dNTPs and salts.
Enhanced DNA binding affinity and rapidly resuspends
Protocol is 5-10 minutes FEATURES
No phenol/chloroform extraction or alcohol precipitations Available for <20bp or <32bp primers
APPLICATIONS Spin format for rapid purification
PCR clean-up, purifying after in-vitro labeling, restriction enzyme APPLICATIONS
removal or cleaning any DNA preps Remove primers, dNTPs and other decontaminants from PCR
reactions
Cat. No. Description Size
786-87 pinkCLEANUP 200 preps Cat. No. Description Size
786-89 pinkRESIN 1ml 786-174 Spin-OUT PCR-20 10 columns
786-175 Spin-OUT PCR-32 10 columns

12 For further details, visit GBiosciences.com


DNA Cleanup & Concentration
Spin-OUT GET AGAROSE DNA

For desalting and buffer exchange Rapid purification of DNA from agarose gels
The SpinOUT GT-600 and GT-1200 columns are versatile, spin-

This kit is based on our GET Spin Columns, spin columns with a
format columns for the desalting and buffer exchange of nucleic high binding affinity for DNA.
acid solutions ranging from 5l through to 4ml sample volumes. The GET AGAROSE DNA method involves release of DNA fragments
SpinOUT columns are available in two MWCO sizes for nucleic acids from gel pieces followed by capture of nucleic acids on the GET Spin
larger than 10bp or nucleic acids larger than 20bp. Columns, washing, and elution of the clean nucleic acid fragments in
The SpinOUT columns are simply to use as the sample is applied a suitable buffer.
and then centrifuged to recover nucleic acids with the column The GET AGAROSE DNA kits are supplied with enough reagents
retaining >95% of the salts and small molecules (<1,000Da). for 50 or 100 preps.
Spin-OUT GT-600 is for the purification of nucleic acids larger
than 10bp and proteins > 6kDa.
Spin-OUT GT-1200 is for the purification of proteins >30bp and
the removal of molecules >1,500Da.
Five sizes are available for each MWCO and are highlighted below:
FEATURES
5 sizes available for sample volumes of 5l to 4ml
Spin format for rapid purification Figure 28: GET AGAROSE DNA scheme.
CITED REFERENCES
Tripodi, K et al (2005) Plant Physiol. 139:969 FEATURES
Taggert, C. et al (2005) J. Exp. Med. 202:1659 Extract DNA fragments from agarose gels
Resin
Rapid DNA isolation
Bed Sample DNA ready for downstream applications, including ligations
Cat. No. Description Size (ml) Load (ml) Suitable for 100-20,000bp fragments
786-703 SpinOUT GT-600, 0.1ml 25 columns 0.1 0.005-0.02 Compatible with TAE and TBE buffer gels
786-170 SpinOUT GT-600, 1ml 10 columns 1 0.05-0.1 APPLICATIONS
786-171 SpinOUT GT-600, 3ml 10 columns 3 0.1-0.5 Isolation of DNA fragments from agarose gels
786-704 SpinOUT GT-600, 5ml 5 columns 5 0.5-2 Cleaning and removing of contaminants from DNA samples
786-705 SpinOUT GT-600, 10ml 5 columns 10 0.5-4 Cat. No. Description Size
786-706 SpinOUT GT-1200, 0.1ml 25 columns 0.1 0.005-0.02 786-358 GET AGAROSE DNA 50 preps
786-172 SpinOUT GT-1200, 1ml 10 columns 1 0.05-0.1 786-359 GET AGAROSE DNA 100 preps
786-173 SpinOUT GT-1200, 3ml 10 columns 3 0.1-0.5
786-707 SpinOUT GT-1200, 5ml 5 columns 5 0.5-2
geneEXIT
786-708 SpinOUT GT-1200, 10ml 5 columns 10 0.5-4

Isolation of nucleic acids from agarose gels
The kit is based on pinkRESIN, a high capacity, proprietary
binding resin matrix for nucleic acids. pinkRESIN has an enhanced
binding affinity for DNA and RNA and resuspends with ease, thus
eliminating loss and damage of nucleic acids, a frequent problem
with other binding resin matrices for DNA and RNA isolation.
pinkRESIN is a non-toxic matrix.
geneEXIT method involves release of nucleic acid fragments
from gel pieces followed by capture of nucleic acids with pinkRESIN,
washing, and elution of the clean nucleic acid fragments in a suitable
buffer. The geneEXIT method can be carried out with or without the
use of spin columns; however the use of spin columns simplifies the
protocols.
FEATURES
Rapid nucleic acid isolation
DNA & RNA ready for further applications, including ligations
Suitable for 100-20,000bp fragments
Compatible with TAE and TBE buffer gels
Supplied with or without spin columns
APPLICATIONS
Isolation of DNA/RNA fragments from agarose gels
Cleaning contaminants from DNA & RNA samples
Cat. No. Description Size
786-85 geneEXIT 100 preps
786-86 geneEXIT with spin columns 100 preps

For further details, visit GBiosciences.com 13


PCR
DNA Polymerases Pfu Polymerase

Pfu DNA polymerase, derived from the hyperthermophilic archae
Taq Polymerase Pyrococcus furiosus, has superior thermostability and proofreading
properties compared to other thermostable polymerase. Its molecular
A recombinant Taq DNA Polymerase is a highly thermostable weight is 90 kDa. It can amplify DNA target up to 2kb (simple
DNA polymerase isolated from the thermophile, Thermus aquaticus. template). The elongation velocity is 0.2~0.4kb/min(70~75C). Pfu
Taq DNA polymerase catalyzes the 5>3 synthesis of DNA. The DNA polymerase possesses 3' to 5' exonuclease proofreading activity
enzyme has no detectable 3>5 proofreading exonuclease activity, that enables the polymerase to correct nucleotide-misincorporation
and possesses low 5>3 exonuclease activity. The Taq polymerase errors. This means that Pfu DNA polymerase-generated PCR
is able to amplify DNA up to 6kb that have a single adenine 3 fragments will have fewer errors than Taq-generated PCR inserts.
overhang. The error rate of this Taq polymerase is ~2.2x10-5 Using Pfu DNA polymerase in your PCR reactions results in blunt-
nucleotide-1 cycle-1. ended PCR products, which are ideal for cloning into blunt-ended
Unit Definition vectors. Pfu DNA polymerase is superior for techniques that require
high-fidelity DNA synthesis. Supplied with 10X Buffer and 10X
One unit is defined as the amount of the enzyme required to
Loading Buffer.
catalyze the incorporation of 10nmole of dNTPs into an acid-insoluble
form in 30 minutes at 70C using hering sperm DNA as substrate. Unit Definition
Storage Buffer One unit is defined as the amount of the enzyme required to
catalyze the incorporation of 10nmole of dNTPs into an acid-insoluble
20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1%
form in 30 minutes at 70C using hering sperm DNA as substrate.
Nonidet P-40, 0.1% Tween 20, 0.2mg/ml BSA, 50% (v/v) glycerol
Storage Buffer
10X PCR Buffer
20mM TrisCl (pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT, 0.1%
120mM Tris-HCl(pH 8.8), 500mM KCl, 1% Triton X-100, 100mM
Nonidet P-40, 0.1% Tween 20, 0.2mg/ml BSA, 50% (v/v) glycerol
Lycine
10X Pfu Buffer
FEATURES
200mM Tris-HCl(pH8.8@25C), 100mM KCl, 100mM (NH4)2SO4,
5>3 polymerase activity
20mM MgSO4, 1.0% Triton X-100, 1mg/ml BSA
No detectable 3>5 proofreading exonuclease activity
Low 5>3 exonuclease activity FEATURES
Error rate ~2.2x10-5 nucleotide-1 cycle-1 5>3 polymerase activity
Available as a 2X Mastermix 3>5 proofreading exonuclease activity
Available as a 2X Mastermix
Cat. No. Description Size
FEATURES
786-447 Taq Polymerase 1,000U
High-fidelity PCR and primer-extension reactions
786-448 Taq Polymerase 5 x 1,000U
High fidelity PCR for cloning into blunt-ended vectors
Site-directed mutagenesis
Taq Polymerase (2X MasterMix)
Cat. No. Description Size
Taq Polymerase is available as a ready-to-use mixture of the 786-816 Pfu Polymerase 500U
polymerase enzyme, deoxynucleotides (dNTPs) and a 2X reaction
buffer. The 2X MasterMix contains all the necessary reagents for Pfu Polymerase (2X MasterMix)
successful amplification, except for the DNA template and primers.
FEATURES Pfu Polymerase (2X MasterMix) is a premixed, ready-to-use
Ready to use mastermix, just add template & primers solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and
5>3 polymerase activity Reaction Buffer at optimal concentrations for efficient amplification
No detectable 3>5 proofreading exonuclease activity of DNA templates by PCR. To prepare the final PCR, only primers and
Error rate ~2.2x10-5 nucleotide-1 cycle-1 template DNA are added. Pfu Polymerase (2X MasterMix) contributes
to highly reproducible PCR by reducing the risk of pipetting errors,
Cat. No. Description Size miscalculation and contamination. It also contributes to higher
786-449 2X MasterMix Taq Polymerase 100 reactions sensitivity by adding intensifier and optimizer.
FEATURES
5>3 polymerase activity
3>5 proofreading exonuclease activity
Convenient: Pfu DNA Polymerase in a ready-to-use Mix
High yields of PCR products with minimal optimization
Fast: Saves time due to reduced number of pipetting steps
Reproducible: Lower contamination and pipetting error risk
Cat. No. Description Size
786-817 Pfu Polymerase (2X MasterMix) 40 reactions

14 For further details, visit GBiosciences.com


PCR
Deoxynucleotides

Deoxynucleotides (dNTPs) Set

The set consists of 100mM aqueous solutions of dATP, dCTP,


dGTP and dTTP each supplied in a separate vial.
Since the nucleotides are provided separately, the dNTP Set offers
maximum flexibility in preparation of reaction mixes for different
applications. Each deoxynucleotide is also available separately at a
100mM concentration.
ApplicationS
PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA
sequencing, DNA labeling
Cat. No. Description Size
786-460 dNTP Set [100mM] 4 x 0.25ml
786-465 dATP [100mM] 0.25ml
786-466 dCTP [100mM] 0.25ml
786-467 dGTP [100mM] 0.25ml
786-468 dTTP [100mM] 0.25ml

Deoxynucleotides (dNTPs) Mix

Aqueous mixture of dATP, dCTP, dGTP and dTTP. The dNTP Mix is a
ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP,
each at a final concentration of 10 or 25mM. The Mix reduces the
number of pipeting steps and the risk of errors.
Applications
Ready to use in PCR, long-PCR, RT-PCR, cDNA synthesis, primer
extention and DNA labeling.
Cat. No. Description Size
786-443 Deoxynucleotide Mix [10mm], 0.5ml 5mole
786-442 Deoxynucleotide Mix [10mm], 5 x 0.5ml 25mole
786-457 Deoxynucleotide Mix [25mM], 1ml 25mole
786-458 Deoxynucleotide Mix [25mM], 5ml 125mole
786-459 Deoxynucleotide Mix [25mM], 25ml 250mole

PCR Clean Up

Spin-OUT for PCR

Remove contaminants after PCR


Spin format desalting columns that have the ability to remove
salts, radioisotpes, dyes, primers and deoxynucleotides (dNTPs)
for nucleic acids following PCR. Two sizes are available to remove
contaminating products from PCR products, including <20bp primers,
dNTPs and salts or from <32bp primers, dNTPs and salts.
Spin-OUT PCR is for the cleaning of PCR products. PCR-20
removes contaminating products from PCR products, including
<20bp primers, dNTPs and salts. PCR-32 removes PCR products
from <32bp primers, dNTPs and salts.
FEATURES
Available for <20bp or <32bp primers
Spin format for rapid purification
APPLICATIONS
Remove primers, dNTPs and odecontaminants from PCR reactions
Cat. No. Description Size
786-174 Spin-OUT PCR-20 10 columns
786-175 Spin-OUT PCR-32 10 columns

For further details, visit GBiosciences.com 15


RNA Purification
A selection of RNA products for the isolation of RNA and the detection,
Arrest Extraction Buffer
decontamination and digestion of RNase.

A chaotropic RNA extraction buffer
Tri-Xtract
An optimized combination of various chaotropic agents and
For RNA free of protein and DNA contamination RNase inhibitors, which inhibits RNase in 5-10 seconds. Arrest
Extraction Buffer may be used in conjunction with any RNA extraction
Tri-Xtract is a convenient, ready-to-use reagent designed
method, including extractions based on phenol, chloroform and other
for the isolation of total RNA that is free from protein and DNA
organic solvents and detergents.
contamination. The isolated RNA is suitable for Northern blots, dot
The quick 10 minute single step protocol isolates high quality
blot hybridization, in-vitro translation, RNase protection assays and
total RNA from most species and tissues. Protocol involves
poly (A+) selection.
homogenization and lysis of samples in Arrest Buffer. After removing
Tri-Xtract is a monophasic solution of phenol and guanidine
cellular debris, pure RNA is precipitated from the supernatant with
thiocyanate that maintains the integrity of the RNA during cell
ethanol. Conveniently packaged as two 50ml bottles for isolation
disruption and homogenization, the addition of chloroform results in
from up to 10 grams of tissue.
the separation of the homogenate into aqueous and organic phases.
RNA partitions to the aqueous phase, DNA to the interphase, and FEATURES
proteins to the organic phase. The total RNA is recovered by isopropyl Rapidly inhibit destructive RNases
alcohol precipitation. The DNA and proteins can also be recovered by Strong chaotropic buffer for rapid RNA release
sequential precipitation from the organic phase. Compatible with numerous species and tissues
Suitable for 200 x 50mg extractions
Cat. No. Description Size
786-133 Arrest Extraction Buffer 200 preps

GET Total RNA



Small scale, spin format RNA purification
The GET Total RNA kit isolates total RNA from contaminating
DNA, proteins, and nucleases using our GET RNA Spin Columns.
The <60-minute protocol is simple; after homogenization, RNA is
bound to the GET RNA Spin Columns and washed. The protocol
provides an option to remove contaminating DNA with a single DNase
treatment. Finally RNA is eluted from the column. The eluted RNA is
ready for any procedure including Northern/slot/dot blots, reverse
transcription or RNase protective assays.
Three step protocol:
1. Arrest Extraction Buffer lyses samples instantly destroying
RNase activity.
2. GET RNA Spin Columns captures RNA.
3. After a brief wash step, protein free RNA is eluted.

Figure 29: Tri-Xtract scheme.


Tri-Xtract is suitable for the isolation of total RNA, including

mRNA, hnRNA, ribosomal RNA and tRNA, from small quantities of


tissues (50-100mg) and cells (5 x 106) from human, animal, plant,
yeast, bacterial and viral origin. Total RNA is isolated in under an
hour and the subsequent isolation of DNA and protein in less than
three hours.
FEATURES
Isolate high quality RNA
Immediate inhibition of RNase
Figure 30: GET Total RNA scheme.
One hour protocol
Scalable FEATURES
Ability to sequentially isolate RNA, DNA then protein Rapidly inhibit destructive RNases
APPLICATIONS Strong chaotropic buffer for rapid RNA release
RNA suitable for Northern blots, dot blot hybridization, in-vitro High affinity RNA binding spin columns
translation, RNase protection assays and poly (A+) selection Optional DNase treatment
Compatible with numerous species and tissues
Cat. No. Description Size Suitable for 50 x 50mg extractions
786-652 Tri-Xtract 100ml
APPLICATIONS
786-653 Tri-Xtract 200ml
For the extraction & purification of protein & DNA free RNA
Cat. No. Description Size
786-132 GET Total RNA 50 preps

16 For further details, visit GBiosciences.com


RNA Purification
TOTAL Arrest RNase-DETECT

Isolation of RNA free of protein & DNA For the detection of RNase contamination
contamination for RT-PCR RNase-DETECT is a highly reliable and sensitive method to detect
The TOTAL Arrest RNA kit isolates RNA from contaminating RNase contamination, which does not utilize unreliable, tedious and
DNA, proteins, and nucleases using our proprietary binding matrix, expensive test strips or radioactive methods.
pinkRESIN. The absence of phenol/chloroform extractions make With RNase-DETECT, 10l of test solution is added to our
the Total Arrest RNA kit one of the safest methods for isolating calibrated RNA substrate vial, incubated, and the result viewed after
and purifying high quality RNA. The 60 minute protocol (micro kit) 10 minutes by agarose electrophoresis.
is simple; after homogenization, RNA is bound to pinkRESIN and
washed. The protocol provides an option to remove contaminating
DNA with a single DNase treatment. Finally RNA is eluted from
pinkRESIN. The eluted RNA is ready for further applications,
including Northern and dot blots, reverse transcription or RNase
protective assays. The kit is supplied in two formats, the Micro for 50
Figure 32: The detection of RNase contamination with RNase-DETECT. The two test
preps (10-50mg tissue/ prep) and Large for 10 preps (100-500mg
samples were compared to the control, showing varying degrees of RNA decontamination.
tissue/ prep).
Three step protocol: FEATURES
1. Arrest Extraction Buffer lyses samples instantly destroying Contains aliquots of calibrated proprietary RNase substrate for
RNase activity. detection of femtogram levels of RNase contamination
2. pinkRESIN captures RNA. Serial Dilution Ladder (SDL) detection technique supplied for rapid,
3. After a brief washing step, pure and protein free RNA is clear detection of contamination
eluted. Compatible with a wide variety of reagent solutions, including SDS,
Tris, salts, agarose, and so forth
Suitable to assay solutions that can not be treated with DEPC
Supplied with RNase substrate vials, reaction-loading dyes, and
easy to follow protocol for a quick analysis of the results
APPLICATIONS
For the detection of RNase contamination in a variety of solutions
Cat. No. Description Size
786-115 RNase-DETECT 56 tests
786-116 RNase-DETECT 112 tests

Figure 31: TotalARREST scheme. RNaseOUT



FEATURES Kills RNase on contact
Rapidly inhibit destructive RNases RNaseOUT is uniquely formulated to destroy and remove RNases
Strong chaotropic buffer for rapid RNA release on contact, simply spray and rinse. RNaseOUT is non-toxic and
High affinity RNA binding matrix to capture RNA residue free. RNaseOUT allows common equipment to be safely and
Optional DNase treatment confidently used for all RNA work.
Compatible with numerous species and tissues RNaseOUT is supplied in 250ml easy to apply spray bottles,
Suitable for animal and plant tissues, cultured cells, blood and simply spray on items or area to decontaminate and rinse. Spraying
bacteria prevents waste and is designed to uniformly cover the application
Suitable for 50 x 50mg (Micro) or 10 x 500mg (Large) extractions area.
APPLICATIONS For economy, refilling spray bottles, or cleaning large items,
For the extraction and purification of protein and DNA free RNA RNaseOUT is also available in 1 liter refill bottles.
Cat. No. Description Size Cat. No. Description Size
786-130 Total Arrest RNA (Micro) 50 preps 786-70 RNaseOUT 250ml Spray
786-131 Total Arrest RNA (Medi) 10 preps 786-71 RNaseOUT 1L Refill

RNasin

RNase Inhibitor
RNasin is a ribonuclease inhibitor extracted from human placenta
with a molecular weight 51kDa. It inhibits the activity of RNase by
specifically binding up to RNase with a non-covalent bond. RNasin,
free of RNase or Nickase, can maintain its activity at pH from 5 to 8,
and the highest one at pH7.8. Concentration is 20~40 units/l.
Cat. No. Description Size
786-815 RNasin [20-40U/l] 1,000 units

For further details, visit GBiosciences.com 17


Nucleic Acid Assay
NUCLEIC dotMETRIC Assay FEATURES
Sensitive, with lower detection limit of 1-5ng/l nucleic acid
A 1l assay for rapid estimation of DNA, RNA and Only 1l sample required
Rapid 3 minute assay
oligo concentrations Suitable for 300 assays
The system uses a unique combination of proprietary APPLICATIONS
reagents and test strips and is a cost-effective alternative to UV
Estimation of DNA, RNA & oligonucleotide concentrations
spectrophotometry as no expensive equipment or cuvettes are
Assay for precious samples, requires only 1l sample
necessary. NUCLEIC dotMETRIC uses as little as a microliter of your
sample and provides permanent results in minutes. ACCESSORIES
Samples in the range of 10g/l to 1ng/l can be measured Spot Application Device: Simplifies the application of samples
accurately regardless of the isolation method or storage buffer. 1l Application glass capillary tips
Oligonucleotides as short as 16 bases have successfully been 1-10l Sample Application (pipette) Tips for standard pipettes
measured with NUCLEIC dotMETRIC. Measurements are not Developing Trays
affected by protein contamination in the sample. The lower detection CITED References
limit of the system is 1-2ng/l. Akopiants, K. et al (2005) J. Ind. Microbiol. Biotechnol. 33: 141
Samples are diluted with dilution buffer and 1-5l spotted onto Nakashima, A., et al (2002) J. Biochem. Soc. 131: 391
Weghofer, M. et al (2001) Ann. Hematol. 80: 733
the NUCLEIC Test Strip, which are developed with NUCLEIC Dye Campbell, T. B et al (2000) AIDS. 14:2109
in under 2 minutes. The dotMETRIC scale and spot diameter are
compared for accurate nucleic acid concentrations. Cat. No. Description Size
For increased reproducibility and test reliability the kits are 786-60 NUCLEIC dotMETRIC >300 assays
supplied with an optional Spot Application Device. This allows 786-61 NUCLEIC dotMETRIC with Spot Application Device >300 assays
application of samples using fixed volume capillary tips, simplifying 786-63 dotMETRIC Spot Application Device 1
the application of the nucleic acid solution and improving the 786-23 1l Application Glass Capillary Tips 100
reliability of results. 786-24 Developing Trays 2
The assay is unaffected by the presence of common laboratory 786-64 Sample Application (pipette) Tips 96 tips
agents, such as reducing agents, chelating agents, detergents,
amines, sugars, chaotropes, salts and other common laboratory
agents.

Figure 33: NUCLEIC dotMETRIC scheme.

18 For further details, visit GBiosciences.com


Nucleic Acid Electrophoresis
Geno-ElectroPhore Power Supplies

A mini horizontal gel electrophoresis system
A simple and economical device for a variety of agarose GT 300 Power Pack
electrophoresis applications, supplied with gel casting tray, combs
and horizontal tank. The Complete-Electrophore is also supplied Programmable power supply for electrophoresis
with a mini power supply. A versatile microprocessor, programmable power supply unit for
The ultimate in simplicity and convenience in casting agarose vertical and horizontal gel electrophoresis.
gels, light-weight and simple to use. The gel casting tray is designed FEATURES
to eliminate the leak problems common with other gel casting trays.
Compact and stackable unit
The device allows casting of agarose gels in two sizes: two 5 x 8.3cm
Power capacity: 300 volts, 400mA and 100 watts
mini gels and one 10.5 x 8.3cm gel.
Constant voltage or constant current
The casting trays are provided with background black strips for
10 programmable methods available
high well visibility. Supplied with two mini gel trays, one large tray and
Timer with alarm function
two different size combs. The combs provide either 13 and 6 wells
Safety features: No load detection, shrouded plugs and sockets
(0.6 x 0.1cm well) or 17 and 8 wells (0.4 x 0.1cm well) for the large
Suitable for 100-240V
and mini gels, respectively. Additional Gel Casting sets that include
APPLICATIONS
the casting tray, two mini gel trays, one large tray and two different
Programmable power supply for horizontal agarose or vertical
size combs are also available.
polyacrylamide electrophoresis systems
Interlocked ventilated fog free lid allows full view of gel during
electrophoresis. Cat. No. Description Size
Complete-Electrophore GT-300 GT 300 Power Pack 1
Supplied with a power supply unit in addition to Geno- MT-P01 Additional power cord 1
Electrophore. The power supply is suitable for most agarose
electrophoresis applications. Mini-300 Power Supply

Programmable power supply for electrophoresis
The Mini 300 power supply is capable of providing constant
voltage or constant current in 1V or 1mA steps, the unit is perfectly
suited to run both vertical polyacrylamide or horizontal agarose gel
electrophoresis experiments.
Continuous or timed operation are easily performed using
the simple and user-friendly interface. The Mini 300 power
supply features 2 electrode pairs, allowing for 2 gels to be run
simultaneously, saving both time and valuable bench space. With a
universal voltage rating, the Mini 300 power supply is also designed
and constructed to the most rigorous safety standards.
FEATURES
Constant voltage or constant current operation
Figure 34: Complete-Electrophore 1V step voltage selection; 1mA step current selection
400mA maximum current
FEATURES 60W maximum power
Eliminates gel leaking issues, no taping required Two pairs of outlet terminals
Cast two gel sizes; two 5 x 8.3cm and one 10.5 x 8.3cm gels Timer with alarm function
Designed for easy well identification Safety device
Two combs provided for small and large wells Compact size and lightweight
APPLICATIONS APPLICATIONS
A complete horizontal agarose electrophoresis system Sufficient for midi sized horizontal and vertical electrophoresis
systems
Cat. No.
Description Size
MT-108 Geno-Electrophore 1 Cat. No. Description Size
MT-109 Complete-Electrophore, MT-108 with power supply 1 EP-300 Mini-300 Power Supply 1
EP-300 Mini-300 Power Supply 1
MT-C01 Comb for 1 x 4mm wells 1
MT-C02 Comb for 1 x 6mm wells 1
MT-T01 Large gel tray (10.5 x 8.3cm) 1
MT-T02 Mini gel tray (5.0 x 8.3cm) 1
MT-T03 Casting Tray 1
Gel Casting Set
MT-T04 1
(Casting tray, 2 mini gel trays, large tray & 2 combs)

For further details, visit GBiosciences.com 19


Nucleic Acid Electrophoresis
G-CAPSULE GET AGAROSE DNA

Electroelution device for the rapid purification of Rapid purification of DNA from agarose gels
nucleic acids from electrophoresis gels This kit is based on our GET Spin Columns, spin columns with a
Electroelution of nucleic acids and proteins has many advantages high binding affinity for DNA.
as it avoids centrifugation, vortexing, heating, precipitation and GET AGAROSE DNA method involves release of DNA fragments
allows minimal manipulation of samples. Electroelution normally from gel pieces followed by capture of nucleic acids on the GET Spin
involves dialysis tubing, which results in extreme dilution of precious Columns, washing, and elution of the clean nucleic acid fragments in
samples. G-Capsule is a simple electroelution device that excises a suitable buffer.
DNA or protein bands and elutes your sample in a final volume of The GET AGAROSE DNA kits are supplied with enough reagents
approximately 30l. for 50 or 100 preps.
G-CAPSULE has two parts: G-Pick and G-Trap. The user
simply picks up the protein or nucleic acid band with the G-Pick
and assembles it with the G-Trap. The assembled G-CAPSULE is
submerged in electrophoresis buffer on a horizontal electrophoresis
system and the protein or nucleic acid is rapidly eluted into the
G-Trap.

Figure 37: GET AGAROSE DNA scheme

FEATURES
Extract DNA fragments from agarose gels
Rapid DNA isolation
DNA ready for downstream applications, including ligations
Suitable for 100-20,000bp fragments
Compatible with TAE and TBE buffer gels
Figure 35: The G-CAPSULE procedure APPLICATIONS
Isolation of DNA fragments from agarose gels
FEATURES Cleaning and removing of contaminants from DNA samples
Rapid electroelution of nucleic acids and proteins
Cat. No. Description Size
Sample recovered in a small volume (25-50l)
Recovery is as high as 90% 786-358 GET AGAROSE DNA 50 preps
786-359 GET AGAROSE DNA 100 preps
APPLICATIONS
Extraction of >20bp DNA and RNA or for >4kDa proteins
geneEXIT
ACCESSORIES
G-CAPSULE Weight: Prevents G-CAPSULE from floating during

Isolation of nucleic acids from agarose gels
electroelution
The kit is based on pinkRESIN, a high capacity, proprietary
binding resin matrix for nucleic acids. pinkRESIN has an enhanced
binding affinity for DNA and RNA and resuspends with ease, thus
eliminating loss and damage of nucleic acids, a frequent problem
with other binding resin matrices for DNA and RNA isolation.
pinkRESIN is a non-toxic matrix.
geneEXIT method involves release of nucleic acid fragments
from gel pieces followed by capture of nucleic acids with pinkRESIN,
washing, and elution of the clean nucleic acid fragments in a suitable
buffer. The geneEXIT method can be carried out with or without the
use of spin columns; however the use of spin columns simplifies the
Figure 36: The G-CAPSULE weight protocols.
Cited References FEATURES
Chatterjee, S et al (2012) Acta Biochim Biophys Sin. 259:68 Rapid nucleic acid isolation
Chatterjee, S et al (2012) Acta Biochim Biophys Sin. 44:259 DNA & RNA ready for further applications, including ligations
Chatterjee, S et al (2012) Acta Biochim Biophys Sin. 10:1093 Suitable for 100-20,000bp fragments
Cardi, D et al (2010) J Biol Chem. 285:26406
Crosslin, J (2009) HortScience. 44:1790 Compatible with TAE and TBE buffer gels
Li, X et al (2004) Euro J of Phycology. 39:73 Supplied with or without spin columns
Beeson, K et al (2002) Microbiology. 148:179
Yeager, M et al (2001) Circ Res. 88:2e
APPLICATIONS
Robu, M et al (2001) PNAS. 98:8211 Isolation of DNA/RNA fragments from agarose gels
Dudley, E et al (2001) Microbiology. 147:215 Cleaning contaminants from DNA & RNA samples
Brezinschek, Hans-Peter et al (2000) Int Immunol. 12:767
Tanaka, K et al (1999) Yeast. 15:1133 Cat. No. Description Size
Cat. No. Description Size 786-85 geneEXIT 100 preps
786-001 G-CAPSULE 55/box 786-86 geneEXIT with spin columns 100 preps
786-004 G-CAPSULE Weight 1

20 For further details, visit GBiosciences.com


Nucleic Acid Electrophoresis
Glow Dyes The following table highlights the different dyes and SDS present
in the loading dyes. The Glow Dyes are also available without
ethidium bromide (these are the Universal Dyes). All dyes are
DNA/RNA loading dyes with premixed ethidium
bromide to reduce exposure hazards supplied in 1.5ml vials.
Nucleic acids are routinely visualized on agarose gels with the

Bromophenol Blue

Ethidium Bromide
highly toxic ethidium bromide stain. High concentrations of ethidium

Xylene Cyanol
bromide are either added to the molten agarose prior to pouring or
to a large volume of staining buffer to stain the nucleic acids after
electrophoresis. Either method results in a large amount of ethidium
bromide to handle and dispose of.

SDS
Cat. No. Description
The Glow Dyes are designed to minimize the use of ethidium
DNA Glow Dyes [6X]
bromide and the risk associated with the regular use of ethidium
bromide. Glow Dyes have been specifically formulated for nucleic 786-103 Glow BromoBlue Dye Yes No No Yes
acid electrophoresis and contain an optimized concentration of 786-104 Glow CyanoBlue Dye Yes Yes No Yes
buffer agents, tracking dyes and ethidium bromide. These loading 786-105 Glow CleanAway Dye Yes Yes Yes Yes
dyes reduce exposure and many of the problems associated with DNA Universal Dyes [6X]
ethidium bromide use and disposal. Simply add an appropriate 786-100 BromoBlue Universal Dye Yes No No No
volume of Glow Dyes to your sample, load the gel and then visualize 786-101 CyanoBlue Universal Dye Yes Yes No No
with UV; no need for additional ethidium bromide staining or addition 786-102 CleanAway Universal Dye Yes Yes Yes No
of ethidium bromide to the agarose. RNA Glow Dyes [2X]
The DNA Glow Dyes are Ficoll based and are supplied at a 6X 786-107 Glow RNA Dye Yes Yes No Yes
concentration and the RNA Glow Dyes are formamide based and are
RNA Universal Dyes [2X]
at a 2X concentration.
786-106 Universal RNA Dye Yes Yes No No
The DNA and RNA Loading Dyes are offered in multiple formats to
meet all your needs and have variations in the following components:
Dyes: The loading dyes use Bromophenol Blue that migrates at
~300bp (0.7-1.7% agarose) or ~100bp (2.5-3.0% agarose) to
highlight the migration front and Xylene Cyanol FF (CyanoBlue
Loading Dyes) that migrates at ~4,000bp (0.7-1.7% agarose)
or ~800bp (2.5-3.0% agarose) to help resolve higher molecular
weight nucleic acids.
SDS: The detergent SDS (sodium dodecyl sulfate) is supplied in the
CleanAway Loading Dyes and these are recommended for use
with DNA that has a high level of DNA binding proteins. The SDS
eliminates DNA-protein interactions, which prevents poor DNA
migration, DNA sticking to the wells or band shifts. In addition,
SDS treatment prevents long cohesive ends reannealing and Figure 38: DNA/RNA loading dyes
producing artifactual DNA bands.
Ethidium Bromide: The benefits of ethidium bromide in the Glow
Loading Dyes is highlighted above. For researchers who want the
benefits of our loading dyes without the ethidium bromide, try our
Universal Loading Dyes.
FEATURES
Intense DNA/RNA bands with little background or band distortion
Compatible with all agarose or acrylamide gel electrophoresis
Glow RNA Dye is ideal for quick screenings of RNA preps
There is no need to use toxic formaldehyde agarose gels for
checking RNA integrity with Glow RNA Dye
Also available: Universal Loading Dyes containing different tracking
dyes, no ethidium bromide, and the option of added SDS
APPLICATIONS
Suitable as sample loading dye for electrophoresis application
Tracking electrophoresis migration
Visualization of nucleic acids

For further details, visit GBiosciences.com 21


Nucleic Acid Electrophoresis
DNA Ladders

DNAmark 1kb Plus DNA Ladder DNAmark 100bp Ladder

15 DNA bands ranging from 100bp to 10Kb. The 500bp and 11 fragments ranging from 100-1500 base pairs (bp). The 500bp
3,000bp fragment are present at increased intensity to allow easy fragment is present at increased intensity to allow easy identification.
identification. All fragments are precisely quantified and mixed during All fragments are precisely quantified and mixed during the
the production. For 5l loading, all fragments except 500bp and production. For 5l loading, all fragments except 500bp are 40ng.
3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng. The 500bp fragment is 100ng. This ladder is pre-mixed with loading
This ladder is pre-mixed with loading dye and is ready to use. dye and is ready to use.
FEATURE FEATURE
Concentration: 144ng/l Concentration: 100ng/l
Cat. No. Description Size Cat. No. Description Size
786-854 DNAmark 1kb Plus DNA Ladder 50g 786-855 DNAmark 100bp Ladder 50g

DNAmark 500bp DNA Ladder DNAmark 50bp Plus Ladder



10 DNA fragments ranging from 500bp to 5.0kb. The 2,000bp 13 fragments ranging from 50-1000 base pairs (bp). The 250bp
fragment is present at increased intensity to allow easy identification. and 500bp fragment are present at increased intensity to allow easy
All fragments are precisely quantified and mixed during the identification. All fragments are precisely quantified and mixed during
production. For 5l loading, all fragments except 2,000bp are 40ng. the production. For 5l loading, all fragments, except 250bp and
The 2,000bp fragment is 100ng. This ladder is pre-mixed with 500bp, are 40ng. The 250bp and 500bp fragment are 100ng. This
loading dye and is ready to use. ladder is pre-mixed with loading dye and is ready to use.
FEATURE FEATURE
Concentration: 92ng/l Concentration: 128ng/l
Cat. No. Description Size Cat. No. Description Size
786-462 DNAmark 500bp Ladder 50g 786-853 DNAmark 50bp Plus Ladder 50g

DNAmark 100bp Plus Ladder DNAmark 20bp Ladder



14 DNA fragments ranging in size from 100-3000 base pairs (bp).
The 500bp and 1200bp fragment are present at increased intensity 13 fragments ranging from 60-300 base pairs (bp). The 100bp
to allow easy identification. All fragments are precisely quantified and and 200bp fragment are present at increased intensity to allow easy
mixed during the production. For 5l loading, all fragments except identification. All fragments are precisely quantified and mixed during
500bp and 1200bp are 40ng. The 500bp and 1200bp fragment are the production. For 5l loading, all fragments, except 100bp and
100ng. This ladder is pre-mixed with loading dye and is ready to use. 200bp, are 40ng. The 100bp and 200bp fragments are 100ng. This
ladder is pre-mixed with loading dye and is ready to use.
FEATURE
Concentration: 136ng/l FEATURE
Concentration: 128ng/l
Cat. No. Description Size
Cat. No. Description Size
786-856 DNAmark 100bp Plus Ladder 50g
786-852 DNAmark 20bp Ladder 50g

1kb Plus 500bp 100bp Plus 100bp 50bp Plus 20bp


Base Pairs
Base Pairs
10,000 Base Pairs Base Pairs
3,000
8,000 Base Pairs 1,000 300
Base Pairs 2,000
6,000 1,500 900 280
5,000 1,500
5,000 1,000 800 260
4,500 1,200
4,000 900 700 240
4,000 1,000
3,000 800 600 220
3,500 900
2,000 700 500 200
3,000 800
1,500 600 400 180
2,500 700
1,000 500 300 160
2,000 600
700 400 250 140
1,500 500
500 300 200 120
1,000 400
400 200 150 100
500 300
300 100 100 80
200
200 50 60
100
100

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Nucleic Acid Electrophoresis
Accessories, Buffers & Chemicals Ethidium Bromide

Cat. No. Description Size
Electrophoresis Running Buffers
R034
R041
Ethidium Bromide Solution [10mg/ml] 10ml
Ethidium Bromide Solution [0.625mg/ml] 5ml
Molecular grade, concentrated running buffers for DNA and RNA
RC-049 Ethidium Bromide 5g
electrophoresis.
Cat. No. Description Size
786-060 TAE [50X] 0.5L
R023 TAE [50X] 1L FASTsilver
R024 TAE [50X] 1gal
R025 TBE [10X] 1L A rapid silver stain for nucleic acids
R026 TBE [10X] 1gal A nanogram sensitive silver staining kit that produces crystal clear
786-474 TBE-Urea Sample Buffer 30ml background and maximal sensitivity needed for critical analysis.
R031 MOPS Running Buffer [10X] 1L A unique formulation of reagents that leaves the background
R032 MOPS Running Buffer [10X] 1gal clear and produces sharp images of nucleic acid bands. FASTsilver
786-532 MOPS/SDS Running Buffer [20X] 0.5L detects as little as 0.3ng nucleic acid. The kit contains ready to use
reagents for 25 mini-gels and comes with a simple to follow 60-90
minute protocol.
FEATURES

Agarose Powders Stains both nucleic acids and proteins


Produces clear background for maximum visibility
Protocol time 60-90 minutes
Biotechnology grade agarose powders
APPLICATIONS
Cat. No. Description Size Staining of nucleic acids and proteins in electrophoresis gels
RC-122 Agarose I (All purpose agarose) 5g
Cited References
RC-005 Agarose I (All purpose agarose) 25g Reed, D. et al (2005) Plant Cell Reports. 24:15-24
RC-006 Agarose I (All purpose agarose) 100g Ortega, N. et al (2005) Mol. Biol. Cell 16: 3028
Venkatesh, S.G. et al (2004) Am. J. Physiol. Cell Physiol. 286: C365
RC-149 Agarose I (All purpose agarose) 500g
Melody, J. L. et al (2004) J. Anim. Sci. 82: 1195
RC-007 Agarose II (Low melting agarose) 25g Delcroix, J. D. et al (2003) Neuron 39: 69
RC-008 Agarose II (Low melting agarose) 100g Wu, C. et al (2003) Am. J. Respir. Cell Mol. Biol. 2: 731
Kralj, S. et al (2002) Appl. Envir. Microbiol. 68: 4283
RC-009 Agarose III (Pulse field gel electrophoresis) 25g
RC-010 Agarose III (Pulse field gel electrophoresis) 100g Cat. No. Description Size
RC-011 Agarose IV (Highest gel strength) 25g 786-30 FASTsilver 25 mini gels
RC-012 Agarose IV (Highest gel strength) 100g

GELPLATE-clean
LabSafe Nucleic Acid Stain


Spray and wipe; specifically formulated for
LabSafe Nucleic Acid Stain is a new and safe nucleic acid electrophoresis gel plates
stain for the visualization of double-stranded DNA, single-stranded
DNA, and RNA in agarose gels. The dyes are developed to replace Clean your electrophoresis plates with GELPLATE-clean and
toxic Ethidium Bromide (a potent mutagen), commonly used in gel prevent poor electrophoretic band migration, band distortions,
electrophoresis for visualization of nucleic acids in agarose gels. and poor image development. Simply spray and wipe clean your
LabSafe Nucleic Acid Stain is non-carcinogenic by the Ames-test. electrophoresis plates with GELPLATE-clean.
The results are negative in both the mouse marrow chromophilous FEATURES
erythrocyte micronucleus and mouse primary spermatocyte Suitable for sequencing and protein electrophoresis gel plates
chromosomal aberration tests. Removes proteins, nucleic acids, fats, lipids, radioisotopes and
LabSafe Nucleic Acid Stain emits green fluorescence when other contaminants from electrophoresis gel plates
bound to dsDNA and red fluorescence when bound to ssDNA or RNA. Supplied in 2 x 250ml easy to apply spray bottles
It has two excitation wavelength peaks when bound to nucleic acid, Refill solutions are offered separately
at 290nm and 490nm. APPLICATIONS
Cleans sequencing and electrophoresis gel plates
Cat. No. Description Size
786-140 GELPLATE-clean Spray 2 x 250ml
786-140RF GELPLATE-clean Refill 2 x 1L
Figure 39: Plasmid DNA stained with LabSafe Nucleic Acid Stain 786-140RF-I GELPLATE-clean Refill for Solution I 1L
786-140RF-II GELPLATE-clean Refill for Solution II 1L
Cat. No. Description Size
786-409 LabSafe Nucleic Acid Stain [10,000X] 1ml

For further details, visit GBiosciences.com 23


Hybridization Buffers
EKONO

For cleaner backgrounds
EKONO is a high performance hybridization buffer system
providing researchers with unprecedented simplicity, reliability,
accuracy and cost saving for hybridization.
The protocol involves incubation of blots with denatured probes
in our EKONO hybridization buffer, followed by washing of the blot
to remove excess unhybridized probes. Easy-to-use EKONO provides
high resolution and sensitivity.
Suitable for Southern/Northern or Southwestern hybridization
analysis, hybridization with oligonucleotides or other types of probes,
and for hybridization screening of gene libraries.

CITED REFERENCES
Guo, H. et al (2010) Antimicrob. Agents Chemother. doi:10.1128/AAC.00989
Guo, H. et al (2007) J. Virology 81: 10072
Guo, H. et al (2007) J. Virology 81: 12472
Dougherty, A. M. et al (2007) Antimicrob. Agents Chemother. 51:4427
Hodson, J. et al (2003). Nuc. Acid Res. e31: e134
Kim, Mee-Jung et al (2002) PNAS. 99: 10096
Moraleda, G. and Taylor, J. (2001) J. Virol. 75: 10161

Cat. No. Description Size


786-160 EKONO 1L

HYB-LINK

Formamide-free hybridization buffer
Hyb-LINK is a high performance formamide free hybridization
solution for Northern, Southern and Dot Blots, which increases
sensitivity of random-primer DNA probes. Hyb-LINK capitalizes
on increased sensitivity of blot hybridization without increasing
background and therefore provides reliable and accurate
hybridization.
Hyb-LINK is compatible with both RNA and DNA probes.
Cat. No. Description Size
786-405 Hyb-LINK 125ml
786-406 Hyb-LINK 4 x 125ml

Other Hybridization Buffers



Cat. No. Description Size
786-558 Church & Gilberts [2X] 250ml
786-559 Church & Gilberts [2X] 0.5L
786-525 Denhardt Solution [100X] 50ml
R015 Hybridization Denaturing Solution 1L
R016 Hybridization Denaturing Solution 1gal
R017 Hybridization Neutralizing Solution 1L
R018 Hybridization Neutralizing Solution 1gal
786-528 Hybridization Solution I 1L
786-023 SSC [20X] 0.5L
R019 SSC [20X] 1L
R020 SSC [20X] 1gal
786-024 SSPE [20X] 0.5L
R021 SSPE [20X] 1L
R022 SSPE [20X] 1gal
786-538 TMAC Hyb Solution [3X] 1L
786-470 TNT Buffer 100ml
786-471 TNT Buffer 500ml

24 For further details, visit GBiosciences.com


Yeast Research Tools
EZ Yeast Plasmid Prep Fast Yeast Transformation

A simple yeast plasmid miniprep method For the rapid transformation of yeast
The EZ Yeast Plasmid Prep kit is designed for the rapid isolation

Fast Yeast Transformation kit is a rapid single step yeast
of 2 plasmids from yeast patches or yeast grown in small liquid transformation kit that takes less than 10 minutes to prepare
cultures. The kit is adapted from the alkaline lysis of E. coli, competent yeast cells. The competent yeast cells can be used
by providing modified alkaline lysis buffers and our LongLife immediately or frozen for later use. This method is suitable for both
Zymolyase, a highly stable enzyme. The EZ Yeast Plasmid Prep kit circular and linear plasmid transformations. The protocol involves
makes it possible to isolate high yield yeast plasmid without the use simply suspending yeast cells in the supplied Competent Buffer and
of glass beads, phenol, or repeated vortexing. the cells are then ready to receive transforming DNA. Introduce DNA
LongLife Zymolyase, a high performance lytic enzyme to the competent cells and incubate for transformation.
preparation, efficiently releases plasmids with a yield of up to 0.1- FEATURES
0.3ng for most 2-based plasmids from a 1.5ml culture (1 prep). High transformation efficiency: 105-106 transformants/ g circular
EZ Yeast Plasmid Prep is suitable even for low copy number yeast DNA
plasmids. Broad spectrum: Compatible with C. albicans, S. pombe, P.
The kit can be used for plasmid isolation from colonies, patches pastoris, or S. cerevisiae
on plate, or liquid culture. The recovered plasmid is in TE buffer and Transformation procedure takes less than an hour
ready for use in any molecular biology application such as E. coli Frozen competent cells are good for use for up to 6 months
transformations, PCR, etc. Simple protocol for multiple plasmid transformation
APPLICATIONS
For the generation of competent yeast cells
Generates plasmids suitable for multiple downstream applications
CITED REFERENCES
Ito, J. et al (2011) Plant Cell Physiol. 52:539
Yang, J. et al (2011) Mol. Microbiol. 79:872
Kim, J. et al (2011) Plant Cell Physiol. 52:2136
Takano, S. et al. (2010) Plant Cell Physiol. 51:62
Kikis, E. A. et al (2009) PLoS Genet 5(1):e1000352
Aihara, T et al (2009) Biol. Reprod. 80:762
Hayashi, C., et al (2008) J. Biol Chem. 283:14801
Morokuma, Y. et al (2007) J. Biol. Chem. 282:24806
Lin, Y. F. et al (2007) J. Biol. Chem. 282:16783
Ono, Y et al. (2006) J. Biol. Chem.281:18519
Lin, Y. et al. (2006) PNAS. 103:15617
Ueki, N and Hayman, M.J. (2003) J. Biol. Chem. 278:24858
Figure 40: The EZ Yeast Plasmid Prep scheme. Uhl, M. et al (2003) EMBO J. 22:2668
Xia, H. et al (2003) Plant Cell 15:449
Jia, Huu-Jun et al (2003) Plant Cell. 15:449
FEATURES Asawatreratanakul, K. et al (2003) Eur. J. Biochem. 270:4671
Minimize time to analyze yeast plasmids; no need to shuttle Osman, A. et al (2001) J. Biol. Chem. 276:10072
plasmids to E.coli
Yield of 0.1-0.3ng/ 1.5ml culture Cat. No. Description Size
No requirement for glass beads, phenol or excessive vortexing GZ-1 Fast Yeast Transformation 120 preps
Suitable for colonies, patches and liquid culture
Purified plasmid DNA suitable for PCR amplification, Yeast Related Buffers
transformations and other downstream applications
APPLICATIONS Quality DNase and RNase free reagent solutions.
Extraction of 2 plasmids from yeast Cat. No. Description Size
Generates plasmids suitable for multiple downstream applications R038 Sorbitol solution [1M] 100ml
CITED REFERENCES R039 Lithium acetate [1M] 100ml
Baldwin, E. et al (2005) Nuc. Acid Res. 33: 1021
R040 Calcium chloride [1M] 100ml
Hu, J. et al (2005) Nuc. Acid Res. 33: 3271
Belanger, K. et al (2004) J Biol. Chem. 279: 43530
Sabourin, M. et al (2003) Nuc. Acid Res. 31: 4373
Walikonis, R. S. et al (2001) J. Neurosci. 21: 423

Cat. No. Description Size


GZ-02 EZ Yeast Plasmid Prep 100 preps

For further details, visit GBiosciences.com 25


Yeast Research Tools
Yeast Geno-DNA-Template LongLife Enzyme Preparations

Extraction of high quality genomic DNA from yeast Enzymes regularly used in laboratory applications often require
The Yeast Geno-DNA-Template extraction kit isolates high quality preparation of fresh solution before each use. Making fresh enzyme
genomic DNA from yeast. The kit is based on a two-step lysis of solution for each application is time consuming and wasteful. A wide
yeast cells, using LongLife Zymolyase and LongLife Proteinase K, variety of enzyme preparations in a ready-to-use format are offered.
followed by the removal of proteins and other cellular impurities by LongLife enzyme preparations have a long shelf life and no
precipitation and centrifugation. weighing or buffer preparation is needed; simply take an aliquot
The clean, genomic DNA is collected by precipitation and the high and add in your sample. LongLife enzyme preparations contain
yield of extracted DNA has an average 100kb length and has A260/280 cofactors necessary for optimal enzymatic activity. Supplied in
1.8-2.0. suspension form and when stored properly have a one year shelf life.
The kit is supplied with reagents for extracting DNA from 100 x ENZYMES OFFERED
1.5ml yeast cultures. LongLife Zymolyase for the digestion of yeast & fungal cell walls
LongLife Lysozyme for the digestion of bacterial cell walls
LongLife PE LB Lysozyme for the digestion of bacterial cell walls
and fully compatible with the PE LB buffer system. Reduces
viscosity build-up due to presence of nucleases
LongLife Proteinase K for the digestion of proteins in nucleic acid
preparations
LongLife Nuclease for the removal of nucleic acids
LongLife RNase for the digestion of RNA
LongLife DNase for the digestion of DNA

Figure 41: Yeast Geno-DNA-Template scheme

FEATURES
For extraction of genomic DNA from yeast cultures
Compatible with PCR and other downstream applications
APPLICATIONS
Designed for the preparation of high yield, genomic DNA template
from yeast
Cat. No. Description Size
786-134 Yeast Geno-DNA-Template 100 preps

LongLife Zymolyase
Figure 43: LongLife Enzymes are highly stable. Each enzyme preparation
A high performance LongLife Zymolyase preparation is supplied was tested over a period of 4 weeks at 37C & compared with LongLife
in a ready-to-use solution form. The enzyme contains high yeast lytic enzyme preparations stored at -20C. LongLifeZymolyase (1.5units/l)
activity with low non-specific activity. Suitable for yeast cell lysis, was tested with freshly grown yeast suspension by monitoring the decrease in
spheroplast preparation, glucan hydrolysis and more. The enzyme absorbance of the suspension. LongLife Lysozyme (1500units/l) was tested
preparation has been stabilized and can be stored at -20C. by monitoring the decrease in the absorbance of Micrococcus lysodeikticus
suspension. LongLife Proteinase K (5mg/ml) was assayed with our Protease
Assay kit (Cat. No. 82023-260). No measurable loss of activity was noticed.

Cited References
LongLife RNase
Tashiro, R. et al (2010) Crop Sci. 50:1260
LongLife Lysozyme
Butcher, B.G. et al (2011) J Bacteriol. 193:4598
Ermolova, N. et al (2011) Hum.Mol Genet. 20:3331
Markel, E. et al (2011) J Bacteriol. 193:5775
LongLife Proteinase K
Figure 42: LongLife Zymolyase are highly stable. The enzyme Whitaker, V. M. et al (2007) J Amer Soc.Hort Sci.132:534
preparation was tested over a period of 4 weeks at 37C: and compared
with LongLife enzyme preparations stored at -20C. LongLife Cat. No. Description Size
Zymolyase (1.5units/l) was tested with freshly grown yeast suspension 786-036 LongLife Zymolyase [1.5U/l] 2 x 0.5ml
by monitoring the decrease in absorbance of the suspension. 786-037 LongLife Lysozyme [1,500U/l] 2 x 0.5ml
786-042 LongLife PE LB Lysozyme [1,500U/l] 2 x 0.5ml
CITED REFERENCES
Gray, P. et al (2006) Mol Cell Prot. 6:514
786-038 LongLife Proteinase K [5mg/ml] 2 x 0.5ml
Saribas, A. et al (2004) Glycobiology 14:1217 786-039 LongLife Nuclease [10U/l] 2 x 0.5ml
786-040 LongLife RNase [10U/l] 2 x 0.5ml
Cat. No. Description Size
786-041 LongLife DNase 0.5ml
786-036 LongLife Zymolyase 2 x 0.5ml

26 For further details, visit GBiosciences.com


Buffers & Reagents
DNA OUT Molecular Grade Water

DNA OUT is formulated to quickly remove DNA and RNA RNase, DNase & protease free water
contaminations from glass and plastic wares. Simply spray and Molecular Grade Water is suitable for use in molecular
rinse. DNA OUT is non-toxic and leaves no residues to interfere with biology applications which demand a high quality of water and
downstream applications. The fast acting DNA OUT allows laboratory assurance that the water is free from DNase, RNase and protease
equipment and work areas to be made free from DNA contamination. contamination. No toxic agents, such as DEPC, are used in the
Ideal for cleaning microfuge tubes, reaction tubes, PCR machine manufacturing of Molecular Grade Water, eliminating DEPC
surface, pipettes, lab benches, lab equipments, and so forth. interference of enzymatic reactions. Each lot is quality tested for the
DNA OUT is supplied in 250ml easy to apply spray bottles. For absence of RNase, DNase and protease contamination.
economy, refilling spray bottles, or cleaning large items, DNA OUT is
Cited References
also available in 1 liter containers.
Itzek, A. et al (2011) J. Bacteriol. 193:6912
Block, J. et al (2003) J. Anim. Sci. 81:1590
Cat. No. Description Size
McKillip, J. et al (1998) Appl. Envir. Microbiol. 64:4264
786-74 DNA OUT 250ml
786-75 DNA OUT 1L Cat. No. Description Size
786-292 Molecular Grade Water 0.5L
Molecular Biology Buffers & 786-293 Molecular Grade Water 1L
786-72C Molecular Grade Water 1gal
Chemicals 786-73C Molecular Grade Water 4 x 1gal/ case

Molecular Biology Universal Kit DEPC-Treated Water



RNase free water
A selection of 12 x 25ml molecular biology relevant buffers.
These buffers are routinely used in molecular biology and are all For all your RNA research needs. Water is treated with
DNase and RNase free. The buffers supplied are listed below. Diethylpyrocarbonate (DEPC).

Cat. No. Description Size Cat. No. Description Size


R033 Molecular Biology Universal Kit 12 x 25ml 786-109 DEPC-Treated Water 1L
Ammonium acetate [5M] 786-117 DEPC-Treated Water 100ml
Calcium chloride [1M] 786-118 DEPC-Treated Water 500ml
EDTA, pH8.0 [0.5M]
Lithium acetate [1M]
Magnesium chloride [1M] Proteomic Grade Water
Potassium acetate [3M]
SDS [10%] For 2D electrophoresis and mass spectrometry
Sodium acetate, pH5.2 [3M]
Removes worries of protein and dust contamination and
Sodium chloride [5M]
TE Buffer [100X] improves quality and reproducibility of 2D electrophoresis and mass
Tris, pH7.0 [1M] spectrometry results.
Tris, pH8.0 [1M]
Cat. No. Description Size
786-229 Proteomic Grade Water 1L
Research Grade Water

Endotoxin Free Water

Endotoxin, DNA, RNA & protease free water


Endotoxin Free Water is free of endotoxins and enzymes, including
proteases.
Certified tested by the Limulus amebocyte lysate (LAL) test for
endotoxins and determined to be <0.0050EU/ml.
Cat. No. Description Size
786-670 Endotoxin Free Water 0.5L
786-671 Endotoxin Free Water 1L

For further details, visit GBiosciences.com 27


Buffers & Reagents
Buffers & Reagents

RNase and DNase free
A selection of DNase and RNase free buffers designed to ensure
high quality research.
Cat. No. Description Size Cat. No. Description Size
786-493 Acid Citrate Dextrose (ACD) Solution A 25ml R014 SDS [10%] 100ml
786-494 Acid Citrate Dextrose (ACD) Solution B 25ml 786-017 SDS [20%] 1L
RC-004 Agar 1kg 786-016 SDS [20%] 0.5L
RC-003 Agar 500g 786-491 SM Buffer 100ml
R012 Ammonium Acetate [5M] 100ml 786-492 SM Buffer 0.5L
RC-021 Ampicillin Sodium Salt 100g GZ-5S SOB Media 50ml
RC-020 Ampicillin Sodium Salt 25g RC-089 Sodium Acetate 500g
786-524 BBS [2X] 1L RC-090 Sodium Acetate 1kg
786-523 BBS [2X] 0.5L R010 Sodium Acetate, pH5.2 [3M] 100ml
RC-026 Bromophenol Blue (ACS Grade) 25g 786-299 Sodium Azide Solution [1%] 100ml
RC-027 Bromophenol Blue (ACS Grade) 50g RC-091 Sodium Bicarbonate 500g
RC-030 Calcium Chloride (Dihydrate) 500g RC-092 Sodium Bicarbonate 1kg
RC-031 Calcium Chloride (Dihydrate) 1kg RC-125 Sodium Carbonate (Anhydrous) 500g
RC-033 Carbenicillin Disodium Salt 1g RC-126 Sodium Carbonate (Anhydrous) 1kg
RC-032 Carbenicillin Disodium Salt 250mg RC-093 Sodium Chloride 500g
RC-034 Cesium Chloride 100g RC-094 Sodium Chloride 1kg
RC-035 Cesium Chloride 500g R006 Sodium Chloride Solution [5M] 100ml
786-564 CTAB Extraction Buffer 60ml R007 Sodium Hydroxide Solution [2N] 100ml
786-565 CTAB Extraction Buffer 125ml RC-095 Sodium Phosphate (Dibasic) 500g
RC-041 DEPC (Diethylpyrocarbonate) 5g RC-096 Sodium Phosphate (Dibasic) 1kg
RC-042 DEPC (Diethylpyrocarbonate) 25g RC-097 Sodium Phosphate (Monobasic) 500g
786-566 Gram Negative Lysis Buffer 125ml RC-098 Sodium Phosphate (Monobasic) 1kg
786-567 Gram Negative Lysis Buffer 250ml R038 Sorbitol Solution [1M] 100ml
786-579 Gram Negative Lysis Buffer 500ml 786-569 STE (TEN) Buffer [10X] 1L
786-580 Gram Negative Lysis Buffer 1L 786-568 STE (TEN) Buffer [10X] 0.5L
786-529 Imidazole [2M] pH7.5 1L 786-535 STET Solution 0.5L
RC-063 IPTG (Molecular Biology Grade) 10g 786-536 STET-E1 Solution 1L
RC-062 IPTG (Molecular Biology Grade) 1g 786-537 STM Buffer 1L
R036 IPTG (Molecular Biology Grade) 5g R003 TE Buffer [100X] 100ml
R039 Lithium Acetate [1M] 100ml 786-034 TE Buffer [10X] 1L
RC-068 Magnesium Chloride 100g 786-033 TE Buffer [10X] 0.5L
RC-069 Magnesium Chloride 500g RC-103 Tricine 100g
R004 Magnesium chloride [1M] 100ml RC-104 Tricine 500g
786-530 Magnesium Sulfate Solution [1M] 1L RC-105 Tris Base 500g
RC-072 MOPS (3-[N-Morpholino] propane-sulfonic acid) 100g RC-106 Tris Base 1kg
RC-073 MOPS (3-[N-Morpholino] propane-sulfonic acid) 250g RC-107 Tris HCl 500g
786-562 Phenol Red Solution [0.5%] 100ml RC-108 Tris HCl 1kg
786-563 Phenol Red Solution [0.5%] 250ml R001 Tris, pH7.0 [1M] 100ml
R011 Potassium Acetate [3M] 100ml 786-263 Tris, pH7.2 [1M] 100ml
R005 Potassium Chloride Solution [1M] 100ml 786-265 Tris, pH7.5 [1M] 100ml
RC-123 Potassium Chloride, KCl 500g R002 Tris, pH8.0 [1M] 100ml
RC-124 Potassium Chloride, KCl 1kg 786-269 Tris, pH8.5 [1M] 100ml
R008 Potassium Hydroxide Solution [2N] 100ml 786-476 Tris, pH9.0 [1M] 100ml
RC-151 Potassium Iodide, KI 500g RC-109 Tryptone 100g
RC-152 Potassium Iodide, KI 1kg RC-110 Tryptone 500g
RC-081 Potassium Phosphate (Dibasic) 500g RC-112 Urea, Ultrapure Grade 1kg
RC-082 Potassium Phosphate (Dibasic) 1kg BC89 Urea, Ultrapure Grade 500g
786-487 Potassium Phosphate Dibasic Solution [1M] 1L R035 X-Gal (Molecular Biology Grade) 1g
RC-083 Potassium Phosphate (Monobasic) 500g RC-113 X-Gal (Molecular Biology Grade) 100mg
RC-084 Potassium Phosphate (Monobasic) 1kg R037 X-Gluc (Molecular Biology Grade) 100mg
786-488 Potassium Phosphate Monobasic Solution [1M] 1L RC-115 Xylene Cyanol 10g
786-649 RBC Lysis Buffer 100ml RC-116 Yeast Extract 100g
786-650 RBC Lysis Buffer 250ml RC-117 Yeast Extract 500g
786-672 RBC Lysis Buffer 500ml

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G-Biosciences Product Line Overview

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Updated: February 11, 2014 www.GBiosciences.com