Biochemistry

I. Classify the Following Amino Acids.

First Column A. Polar uncharged B. Polar Charged C. Nonpolar
Second Column D. Essential E. Nonessential
Third Column F. Aromatic G. Aliphatic

B D F 1. Histidine A D F 11. Phenylalanine

B E G 2. Glutamic Acid C D G 12. Valine
C E G 3. Alanine A D G 13. Threonine
A E G 4. Serine A E F 14. Tyrosine
C E G 5. Proline A E G 15. Cysteine
B D G 6. Arginine A E G 16. Asparagine
C D G 7. Methionine B D G 17. Lysine
A E G 8. Glutamine B E G 18. Aspartic Acid
C D G 9. Leucine C D G 19. Isoleucine
C D F 10. Tryptophan C E G 20. Glycine

II. Protein Structures. Choose the letter corresponding to your answer.

A B
E

F G
C

21. The bonding present in C 23. The interaction in B

A. Hydrogen Bonding of Side Chain A. Hydrogen Bonding of Side Chain
B. Hydrophobic Interaction B. Hydrophobic Interaction
C. Disulfide Bonding C. Disulfide Bonding
D. Ionic Bonding D. Ionic Bonding
22. Involves Electrostatic Reaction 24. What stabilizes F
A. A A. Hydrogen Bonding
B. E B. Hydrophobic Interactions
C. Both A & E C. Noncovalent Interactions
D. None D. Electrostatic Repulsion
Biochemistry

25. Capable of Interchain hydrogen bonding

A. F C. Both F & G
B. G D. None
III. Peptides

26. Which of the following is not part of the peptide?

A. Arginine
B. Tyrosine
C. Glycine
D. Alanine
E. Lysine
27. What is the net charge of the peptide shown
above?
A. -1
B. 0
C. +1
D. +2
28. The shown ion form of the peptide is most likely
to be present in which of the following pH?
A. pH 5
B. pH 10
C. pH 1
D. pH 14
29. What is the isoelectric pH of the peptide?
A. 5.735 C. 6.575
B. 6.485 D. 7.125
30. What is the net charge of the peptide after its final ionizable group is ionized?
A. -1 D. -4
B. -2 E. -5
C. -3
31. Starting at high pH, if the peptide is titrated with an acid, the ionizable group to react first is?
A. Tyrosines -NH3 D. Arginines -NH3
B. Lysines R-NH3 E. Leucines R-COOH
C. Arginines R-NH3
Biochemistry

32. Which of the following amino-acids is the N-Terminus?

A. E C. C
B. L D. R
33. If the peptide is to fold into a helix the carbonyl group of E will bind to the amino group of ____ via
hydrogen binding?
A. R C. S
B. Y D. E
34. What is the pH of the peptide E-L-L-C-R-Y-S wherein zwitterions exist fully?
A. 6.16 C. 6.49
B. 5.74 D. 6.61
IV. Protein Isolation

Classify the following techniques based on the principle that governs them

A. Based on Size C. Based on Binding Specificity

B. Based on Solubility D. Based on Charge
B 35. Isoelectric Precipitation C 40. Affinity Chromatography
A 36. Centrifugation D 41. Ion Exchange Chromatography
A 37. Ultrafiltration A 42. Dialysis
A 38. Gel Filtration Chromatography 43. High Performance Liquid
C
B 39. Salting-out Chromatography

V. Protein Structures II
A. Collagen C. Both
B. Elastin D. None of the Above

B 44. Random Coil Conformation A 48. Has repeating X-Y-G sequence

B 45. Desmosome crosslinks C 49. Found in connective tissues
A 46. Triple-Helix Structures A 50. Involves hydroxylating enzymes to
C 47. Fibrous Proteins stabilize the protein
Biochemistry

A. Hemoglobin C. Both
B. Myoglobin D. None of the Above

A 51. Tetrahedral arrangement of subunits D 54. Thin long and narrow shaped protein
B 52. Oxygen Storage A 55. Tetramer
C 53. Contains heme

A. Insulin C. Both
B. Glucagon D. None of the Above

B 56. Secreted by the -cells of pancreas B 62. Stimulates breakdown of glycogen to

A 57. Hypoglycemic Agent
B 58. Hyperglycemic Agent
C 59. Maintains homeostasis of glucose
B 60. Stimulates breakdown of lipids to
synthesize glucose
B 61. Stimulates breakdown of proteins to
synthesize glucose
synthesize glucose
A 63. Made up of two polypeptide chains
A 64. Involves disulfide linkage that are
intra and inter chain
C 65. Endocrine hormones