3/1/2017 MicrobiologyMR/VPFlashcards|Quizlet

Microbiology MR/VP 18 terms learlady2003

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Vogues Proskauer What is the name of this test?

Detect bacteria which will What is the purpose of the Methyl Red test?
ferment glucose to form
multiple acids and overcome
the phosphate buffer

Phosphate Buffer What are the ingredients in the MR and VP?

Peptones for protein
Glucose for substrate

Formic Acid What are examples of acids which can be

Lactic Acid produced by a bacteria and detected by the
Acetic Acid Methyl Red test?

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Red coloration after the What is a positive reaction for the MR test?
addition of the Methyl Red
reagent and mixing

Detects bacteria which will What is the purpose of the VP Test?

produce one acid that the
phosphate buffer can neutralize

Acetylmethylcarbinol (AMC) When the single acid detected by the VP test is

neutralized, what is it converted into?

Acetic Acid What are the possible end products for the MR
Formic Acid test?
Lactic Acid

Maroon band at the top of the What is a positive indication in the VP Test?
broth

Barritt's Reagent A (Alpha- What must be added to the VP broth to show a

napthol) reaction?
Barritt's Reagent B (40% KOH)

pH = 4.4 or less What does the pH have to be in the Methyl Red

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test for the broth to turn red with the addition

of the Methyl Red pH indicator?

Alpha-Napthol What is Barritt's Reagent A and Reagent B that

40% KOH is added to Vogues Proskauer test?

Acetylmethylcarbinol What does Barritt's Reagent A and Reagent B

react with in the Vogues Proskauer broth when
the broth turns red?

pH = 6.2 When the acetic acid is converted to

acetylmethylcarbinol, what is the pH of the
broth for the Vogues Proskauer test?

A positive result for the What does the maroon band at the top of the
production of acetic acid. Vogues Proskauer broth indicate?

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Methyl Red What is the name of this test?

They both have the same How are the MR and VP tests alike?
ingredients
Phosphate Buffer
Peptones for protein
Glucose for substrate

The MR test requires the How are the MR and VP tests different?
addition of a pH indicator called
Methyl Red.
The VP test requires the addition
of two reagents to bring about
a color change.

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