BioMag Plus

Superparamagnetic
Particles
BioMagPlus Superparamagnetic Particles
Due to their irregular shape, Superparamagnetic Particles have a large available surface area greater than 20 times that of the same size
spherical particles. BioMagPlus particles are approximately ~1m in size. The small size of BioMagPlus particles provides enormous
binding capacity and fast magnetic separation for the isolation and purification of biological and non-biological molecules.

Amine Particles
High level of amine functionality on magnetically responsive particles. Covalent attachment of proteins can be accomplished via a reaction
with glutaraldehyde. Particles are provided as a suspension at 50mg per ml. Packaging: 10ml volume. See Technical Data Sheet #617.

Amine Protein Coupling Kit

Provides all the reagents necessary for the covalent attachment of proteins to BioMagPlus Amine superparamagnetic
particles. Contents of the kit are sufficient for five coupling reactions. The kit includes: 25ml BioMagPlus Amine, 2x10ml
glutaraldehyde (EM Grade, 25%), 5x50ml conical centrifuge tubes, BioMag MultiSep Magnetic Separator (Cat. #85200-1),
2x125ml Pyridine Wash Buffer (PWB), 2x125ml Quenching Solution (1M Glycine, pH 8.0), and 125ml Wash Buffer.
See Technical Data Sheet #617.

Carboxyl Particles
BioMagPlus Carboxyl superparamagnetic particles are provided for the covalent attachment of proteins using carbodiimide (EDAC
method). These particles are provided as a suspension at 20mg per ml.
Packaging: 10ml volume. See Technical Data Sheet #618.

Carboxyl Protein Coupling Kit

Includes all of the reagents necessary for the covalent attachment of proteins to BioMagPlus Carboxyl superparamagnetic
particles. Contents of the kit are sufficient for five coupling reactions. Kit includes: 2.5ml BioMagPlus Carboxyl, 0.10g EDAC
(1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), 5x15ml conical centrifuge tubes, BioMag MultiSep Magnetic Separator (Cat.
#85200-1), 2x175ml 0.05M MES buffer (pH 5.2), 25ml Quenching Solution (1.0M Glycine, pH 8.0), and 125ml Wash Buffer.
See Technical Data Sheet #618.

Goat anti-Mouse IgG Particles

BioMagPlus Goat anti-Mouse IgG particles are a suspension of BioMagPlus superparamagnetic particles that have goat anti-Mouse IgG
antibody covalently attached. BioMagPlus Goat anti-Mouse IgG particles are suitable for enzyme immunoassays that utilize a mouse IgG primary
monoclonal antibody and in magnetic cell sorting methods that utilize mouse monoclonal antibodies directed to specific cell surface receptors.
Packaging: 50ml volume. See Technical Data Sheet #619.

Goat anti-Mouse IgG Antibody Coupling Kit

The BioMagPlus Goat anti-Mouse IgG Antibody Coupling Kit includes all of the reagents for the attachment of antibodies to BioMagPlus
superparamagnetic particles. The contents of the kit are sufficient for five binding reactions. The kit includes: 25ml BioMagPlus anti-Mouse
IgG, 250ml Coupling/Wash Buffer, 5 x 15ml conical centrifuge tubes and BioMag MultiSep Magnetic Separator (Cat. #85200-1). See
Technical Data Sheet #619

Protein A Particles
Particles with Protein A covalently attached. Isolation of immunoglobulins using BioMagPlus Protein A particles allows isolation from small
samples (50l or less), as well as the option to easily scale up to larger samples. Packaging: 2ml & 10ml volumes. See Technical Data
Sheet #620

Protein A Antibody Isolation Kit

Kit contains all the reagents necessary for the isolation of antibodies from serum and cell culture supernatants. The contents of the kit are
sufficient for five binding reactions. The kit includes: 2.5ml BioMagPlus Protein A, 10 x 1.5ml microcentrifuge tubes, 50ml Protein A Binding/
Wash Buffer, BioMag SoloSep Micro-centrifuge Tube Separator (Cat. #8MB4112S-1), 5ml Protein A Elution Buffer and 1ml Protein A
Neutralization Buffer. See Technical Data Sheet #620.
Protein G Particles
Particles with Protein G covalently attached. Isolation of immunoglobulins using BioMagPlus Protein G
particles allows isolation from small samples (50l or less), as well as the option to easily scale up to larger samples.
Packaging: 2ml & 10 ml volumes. See Technical Data Sheet #620

Protein G Antibody Isolation Kit

The BioMagPlus Protein G Antibody Isolation Kit contains all the reagents necessary for the isolation of antibodies from
serum and cell culture supernatants. The contents of the kit are sufficient for five binding reactions. The kit includes:
2.5ml BioMagPlus Protein G, 10 x 1.5ml microcentrifuge tubes, 50ml Protein G Binding/Wash Buffer, BioMag SoloSep
Microcentrifuge Tube Separator (Cat. #8MB4112S-1), 5ml Protein G Elution Buffer and 1ml Protein G Neutralization Buffer.
See Technical Data Sheet #620

Streptavidin Particles
Particles that are covalently attached to streptavidin. Use these particles for the capture of biotinylated oligonucleotides, proteins, dNTPs
and other molecules. These particles are provided as a suspension at ~5mg per ml.
Packaging: 10ml volume. See Technical Data Sheet #621

Streptavidin / Biotin Binding Kit

Kit contains all of the reagents needed for the attachment of biotinylated proteins to BioMag Plus superparamagnetic
particles. The contents of the kit are sufficient for five coupling reactions. The kit includes: 5ml of BioMagPlus Streptavidin,
250ml Coupling/Wash Buffer, BioMag MultiSep Magnetic Separator (Cat.# 85200-1) and 5 x 15ml conical centrifuge tubes.
See Technical Data Sheet #621

Mouse anti-Fluorescein IgG Particles

Suspension of superparamagnetic particles covalently coupled to Mouse anti-Fluorescein IgG antibody.
Suspension is supplied at 1mg per ml in Phosphate Buffered Saline (pH 7.4) with EDTA and sodium azide
added as stabilizers. Used for the magnetic separation of fluoresceinated cells, components or complexes from solution.
Packaging: 50ml volume. See Technical Data Sheet #692

Wheat Germ Agglutinin (WGA)

Convenient mean for isolating N-acetylglucosamine-containing glycoproteins from cell lysate or to explore other lectin/glycan-
mediated processes. The BioMagPlus magnetic particle format provides high surface area and permits easy and efficient separations.
Packaging: 3ml & 10 ml volumes. See Technical Data Sheet #759.

Concanavalin A (Con A)
Convenient mean for isolating mannosyl and glucosyl-containing glycoproteins and polysaccharides from serum or cell lysate, or for
investigating other lectin/glycan-mediated processes. BioMagPlus magnetic particle format provides high surface area and permits
easy and efficient separations.
Packaging: 3ml & 10 ml volumes. See Technical Data Sheet #766.
 Sound interesting?
For more detailed information on
BioMagPlus Microspheres, go to:
www.polysciences.com/biomagplus
or call (800) 523-2575

polysciences.com

Polysciences, Inc. is a world leader in the development of particle-based solutions for diagnostics, bioprocessing and instrument standardization. We are your source
for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles. Our particle diameters span the range of
40nm to ~10mm. We stand behind our products. Regardless of the size of your question or the size of your company, we offer tech support, absolutely free.

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