Microbial Count on used Musa textilis Fiber Bag

Alelie Nia R. Nabor, Erdjean B. Escoaand Philmar M. Villaruel

Grade 10 Charles, Galileo and Boyle

Regional Science High School for Region VI

Old Buswang, Kalibo,Aklan
School Year 2016-2017

Bacteria can be found in all surface. Some bacteria may lead to many diseases that can also
lead us to death. Many commercial eco-bags were made these days and our own native
products were left behind eventhough they have much better uses like musa textilis fiber
which can be a useful bag because it has an antibacterial properties. The aim of this study
is to encourage the Aklanons to use the abaca fiber bag that contains anti-bacterial
properties that can lessen the growth of bacteria. The researchers came up with the null
hypothesis that states: The presence of anti-bacterial properties of Musa textilis fiber bag
cannot help reduce microbial count when used compared to commercial eco bags and the
study answered that the musa textilis fiber can help the growth of bacteria than the
commercial eco bags. Three replications were made in the study. In the commercial eco
bag. Replication 1 has 198 bacterial colonies, replication 2 has 49 bacterial colonies and
replication 3 has 23 bacterial colonies, while in Musa Textilis Fiber bag replication 1 has
125 bacterial colonies, replication 2 has 25 bacterial colonies, and replication 3 has 11
bacterial colonies. The result shows that abaca fiber bag has less amount of bacterial
colonies than the commercial eco bags. Further study is needed to prove the results. The
use of musa textilis fiber bag is helpful to lessen the growth of bacteria.

Introduction

Philippines is the worlds largest producer of A gelatin-like substance with a semi solid
Musa textilis fiber, accounting for about 85% surface in which the bacteria can grow while
share of the global production in 2013 (Wood consuming other nutrients is the indigestible
L. 2015). Traditionally musa textilis fiber is agar (Science Company, n.d.).
extracted from a leaf health by tearing it up
using either manual or mechanical process. This study aimed to prove that Musa textilis
Itscultivation is suited for the Philippines has an anti-bacterial property which enables
because of the tropical climate and it has a lot to lessen the growth of bacteria. Specifically,
of significance in household and as well as the study determined the difference of
technical areas (Vijayalakshmi K., et.al, microbial counts in between the Musa textilis
2014). The oldest, structurally simple and the fiber bag and commercial eco-bags.
most abundant forms of life on earth is the
bacteria and the only organisms with
prokaryotic cellular organization. Bacteria is
responsible formany kinds of diseases
(Bacteria, n.d.)
.
 Materials and Methods
Gathering of Materials
The materials that were used in the study
includeMusa Textilis fiber bag, commercial eco
bag, apple, banana, tomato, and onion which
were bought from the wet market of Kalibo while
the marker, masking tape, bread, bleach, agar,
cotton swabs and garbage bag were bought from
Gaisano Capital Kalibo and lastly the alcohol
burner, petri dish and test tube were borrowed
from the Chemistry Laboratory of the Regional
Science High School for Region VI.
Preparation for the experiment
Same amount of food were placed in two
different bags and stocked for a week. Materials
such as petri dish, cotton swabs, test tube, marker,
masking tape, bleach, agar, alcohol burner and
garbage bag were then prepared after one week.
Culture Preparation
Prepared petri dishes werestored upside down
and refrigerated until needed to keep
condensation.Let dishes read to room
temperature for one hour before taking samples
Collect bacteria from each bag using one swab or
desterilized inoculating loop for each new spot.
Inoculate each dish by streaking a pattern gently
across the entire agar surface without tearing into
it.
Another common technique is to divide each
plate into four quadrants by marking the lid with
a cross. Streak your sample in straight lines
starting in quadrant 1. Generally, after a few days,
quadrant one will show the most growth.
Depending on bacteria abundance on the swab,
quadrant 4 may show no growth or only a few
colonies. It is sometimes easier to distinguish
different bacteria types in this low growth, less
cluttered area. Replace cover on dish, tape closed,
and label each dish so you know the
source of the bacteria. Store upside down. Let
grow in undisturbed warm location, ideally in an
environment around 100 F (37 C) - not in
sunlight or on a heating register. You should see
growth within a couple of days. The dishes
would, start to smell which means the bacteria
are growing. Make observations and keep
records of what you see growing in each dish.
(Science Company, n.d.)
Culture 1 Culture 2 Culture 3
Musa textilis 16 12 7
Commercial 20 14 7
Eco Bags
Table 1.
Precautionary and Disposal Procedures
Before disposing of the dishes in the trash. the
bacteria should be destroyed. Pour a small
amount of household bleach over the colonies
while holding dish over sink. Caution - do not
allow bleach to touch your skin, eyes or clothes.
It will burn! Most bacteria collected in the
environment will not be harmful. However, once
they multiply into millions of colonies in a petri
dish they become more of a hazard. Be sure to
protect open cuts with rubber gloves and never
ingest or breathe in growing bacteria. Keep
growing petri dishes taped closed until your
experiment is done. Then you should safely
destroy the fuzzy bacteria colonies using
bleach.(Science Company, n.d.)
Research Design
Solution:
The Completely Randomized Design was used Formula for mean:

in the study to have an equal chance in x=
receiving a treatment (Experimental Design).
()2
X= 1
Statistical Tool
Calculate the first set:
The statistical tool used is t-test because it Number of terms in first set:
assesses whether the means of two groups n1=3
are statistically different from each other. This mean for the first set of data:
analysis is appropriate whenever you want to x1=11.67
compare the means of two groups, and especially Construct the following table for standard deviation:
appropriate as the analysis for the posttest-only
two-group randomized experimental design x1 x1-x1 (x1-x1)2
(Research Method Knowledge Base, n.d.) 16 4.33 18.75
12 0.33 0.11
Result
7 -4.67 21.81
Table 1 showed the difference between the 40.67
musa textilis fiber bag and commercial eco bag Standard deviation for the first set of data:
in terms of bacterial growth in 3 to 4 days. S1=4.51
Calculation for the first set:
Culture 1 Culture 2 Culture 3 Number of terms in the second set:
n2=3
Musa textilis 16 12 7 Mean for the second set of data:
x2=40.67
Commercial 20 14 7
Construct the following table for standard deviation:
Eco Bags
x2 x2-x2 (x2-x2)2
20 -20.67 427.25
14 -26.67 711.29
7 -33.67 1133.67
STATISTICAL TOOL 2272.21
(T-test) Standard deviation for the second set of data:
S2=33.71
Musa 16 12 7 Formula for t-test value:
textilis Bag xx
(x1) 1 2
Commercial 20 14 7 +

Eco Bag 11.6740.67
t= 20.34 1136.36
(x2) 3
+
3
t= 19.64
Discussion

The first culture of the commercial eco bag showed

the most number of bacteria while the third culture of
musa textilis fiber bag showed the least number of
bacteria. Compared to the commercial eco bag, the
musa textilis fiber bag showed less bacterial growth.

The 1st culture has lots of bacteria because the area

where the researchers swabbed is the area where the
rotten foods.

Conclusion

Therefor we conclude that the musa textilis fiber bag

is more effective in lessening the microbial count
compared to commercial eco bags.

Bibliography

Wood L.
(2015,January29)http://www.prnewswire.com/news-
releases/philippines-abaca-fiber-market-forecast-
and-opportunities-2019-300028041.html

Experimental Design Research

http://www.mhhe.com/biosci/genbio/raven6b/graphi
cs/raven06b/other/raven06_34.pdf

Method Knowledge Base

http://www.socialresearchmethods.net/kb/stat_t.php

Science Company (n.d.).

https://www.sciencecompany.com/Bacteria-
Growing-Experiments-in-Petri-Plates.aspx

Vijayalakshmi K., et.al, (2014 , September).

http://www.techscripts.org/sep_2014/sep201404
.pdf