Calibration Curve

[PO43-] in ppm Absorbance 1 Absorbance 2

2 0.260 0.314
5 0.369 0.421
10 0.621 0.609
15 0.872 0.821
20 1.260 1.025
25 1.397 1.395

y-intercept 0.1643
slope 0.0475
x-value 3.4589473684

1.6077192982 643.0877192982 1.1305263158

Trial 1 Trial 2
0.239 0.237
Champion Bar 0.231 0.221
0.234 0.229
mean 0.235 0.229
0.176 0.181
Champion Powder 0.169 0.165
0.180 0.182
mean 0.175 0.176
0.154 0.157
Perwoll 0.161 0.151
0.149 0.159
mean 0.155 0.156
urve

Absorbance 3 Absorbance SD uncertainty

0.267 0.280 0.02937 0.0729
0.398 0.396 0.02606 0.0647
0.614 0.615 0.00603 0.0150
0.917 0.870 0.04803 0.1192
1.113 1.133 0.11873 0.2948
1.393 1.395 0.00200 0.0050

0.26 0.215
0.231 0.221
0.231 0.218
0.2406666667 0.218
452.2105263158 1.58486708 31.6973415133
0.45103386 9.0206771188
Trial 3 0.01476937 0.295387412
0.231
0.228
0.238 0.003
0.232 0.232 0.008
0.173
0.179
0.184
0.179 0.177
0.153
0.159
0.154
0.155 0.155
slope 0.048856 0.1544627 b
std dev of slope 0.001205 0.0182733 std dev of intercept
R 0.997571 0.023829 std dev of y
fisher stat 1642.895 4 DOF
regression ss 0.932873 0.0022713 error ss Standard Calib
1.6
t value 2.78 1.4
uncertainty slope 0.003351 f(x) = 0.0488557214x + 0.1544626
1.2
uncertainty b 0.0508 R = 0.9975711871
1
0.8
Absorbance
0.6
0.4
0.2
0
0 5 10

PO4

Champion Bar
Champion Powder
Perwoll
 Standard Calibration Curve

488557214x + 0.1544626866
975711871

10 15 20 25 30

PO43- ppm

Phosphate Concentration
Trial 1 Trial 2 Trial 3
32.788 30.47035 31.83367
8.384458 8.793456 9.884117
0.068166 0.477164 0.340832
 Standard Calibration Curve

Tube Vol. BSA Stock (ml) Vol. DW (ml) Vol. BR (ml) Total Vol. (ml)
1 0.0 1.5 6.0 7.5
2 0.2 0.8 5.0 6.0
3 0.3 0.7 6.0 6.0
4 0.4 0.6 5.0 6.0
5 0.5 0.5 6.0 6.0
6 0.6 0.4 5.0 6.0
7 0.8 0.2 6.0 6.0
8 1.0 0.0 6.0 6.0

Sample
Concentration (with Uncertainty)
True Value %error
Skim Milk
Soy Milk
Non-fat Milk
Whole Milk
Egg
Unknown Sample
e 3

Protein Conc. (mg/ml) Abs1 G2 G3 G4

2.5
0.000 0.545 0.498 0.509 0 f(x) = 55.0234693878x + 0.629
0.0067 1.067 1.113 1.117 0.825 R = 0.9703180221
2 f(x) = 40.1234693878x
40.5632653061x + 0.791
0.751
0.0100 1.301 1.267 1.207 0.988
R = 0.9021173981
0.9175659387
0.0133 1.447 1.399 1.426 1.11
0.0167 1.577 1.586 1.468 1.208 1.5 f(x) = 36.7285714286x + 0.438
A595
R = 0.7545721825
0.0200 1.683 1.634 1.672 1.242
0.0267 1.848 1.961 1.765 1.334 1
0.0333 1.945 2.545 1.984 1.455
0.5

0
0.000 0.005 0.010 0.015

Conc
f(x) = 55.0234693878x + 0.629170068
R = 0.9703180221
f(x) = 40.1234693878x
40.5632653061x + 0.7913367347
0.7512482993
R = 0.9021173981
0.9175659387
f(x) = 36.7285714286x + 0.4387142857
R = 0.7545721825

00 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040

Concentration, mg/mL
 Determination of protein concentration in milk, egg, a

Sample Trial Vol. Sample (ml) Vol. DW (ml) Vol. BR (ml) Total Vol. (ml) Absorbance
1.0 0.2 0.8 5.00 6.00 0.747
Skim milk 2.0 0.2 0.8 5.00 6.00 0.758
3.0 0.2 0.8 5.00 6.00 0.753
average 0.753
1.0 0.2 0.8 5.00 6.00 0.711
Soy milk 2.0 0.2 0.8 5.00 6.00 0.721
3.0 0.2 0.8 5.00 6.00 0.717
average 0.716
1.0 0.2 0.8 5.00 6.00 0.710
Non-fat milk 2.0 0.2 0.8 5.00 6.00 0.701
3.0 0.2 0.8 5.00 6.00 0.692
average 0.701
1.0 0.2 0.8 5.00 6.00 0.720
Whole milk 2.0 0.2 0.8 5.00 6.00 0.711
3.0 0.2 0.8 5.00 6.00 0.717
average 0.716
1.0 0.1 0.9 5.00 6.00 0.559
Egg 2.0 0.1 0.9 5.00 6.00 0.567
3.0 0.1 0.9 5.00 6.00 0.552
average 0.559
1.0 0.2 0.8 5.00 6.00 1.701
Unknown 2.0 0.2 0.8 5.00 6.00 1.671
3.0 0.2 0.8 5.00 6.00 1.659
average 1.677
centration in milk, egg, and unknown sample

SD Uncertainty Protein Conc. (mg/ml) sd uncertainty

8.281
6.225
7.159
0.006 0.0137 7.222 1.030 2.5559 14.4431 36
15.010 28.0263 26
13.141 33.7587 36
13.889 28.1509 30
0.005 0.0125 14.013 2408.9120
15.197 0.1324 0.1330
16.879
18.562
0.009 0.0223 16.879
13.328 7.2215
15.010 14.0132
13.889 16.8793
0.005 0.0114 14.075 14.0755
2412.374 2408.9120
2329.296 0.1324
2485.067
0.008 0.0186 2408.912
0.136
0.132
0.130
0.022 0.0537 0.132
 BSA stock soln (mg/ml) 0.2

R2 0.9021
Slope 40.123
59.8803124836
-7.7935348802 y-intercept 0.7913
6.2258554944 Equation of the Line A595 = 40.123[Protein] + 0.7913
6.1635470927

0.4152456759 Unknown 1
Protein] + 0.7913