URIC ACID -LQ

Uric acid
Uricase -POD. Liquid
SPIN640 APPLICATION

Quantitative determination of uric acid

IVD

TEST INFORMATION
N
Test
Full Name
Standard n

Store at 2-8C
PRINCIPLE OF THE METHOD
Uric acid is oxidized by uricase to allantoine and hydrogen
peroxide (2H2O2), which under the influence of POD, 4
aminophenazone (4-AP) and 2-4 Dichlorophenol sulfonate
(DCPS) forms a red quinoneimine compound:
Uric acid + 2H2 O + O2

Uricase

2H2O2 + 4-AP + DCPS

POD

SAMPLE VOLUME
Vol. Sample Stand.
Vol. Sample Increas.
Vol. Sample Dec

Allantoine + CO2 + 2H2O2

The intensity of the red color formed is proportional to the uric

acid concentration in the sample1,2.

JUDGEMENT CRITERIA
Absorbance
Incre.Test
Decre.Test

CLINICAL SIGNIFICANCE
Uric acid and its salts are end products of the purine metabolism.
With progressive renal insufficiency, there is retention in blood of
urea, creatinine and uric acid.
Elevate uric acid level may be indicative of renal insufficiency and
is commonly associated with gout1,5,6.
Clinical diagnosis should not be made on a single test result; it
should integrate clinical and other laboratory data.
REAGENTS
R1
Phosphate pH 7.4
Buffer
2-4 Dichlorophenol sulfonate (DCPS)
Uricase
R2
Peroxidase (POD)
Enzymes Ascorbate oxidase
4 Aminophenazone (4-AP)

50 mmol/L
4 mmol/L
60 U/L
660 U/L
200 U/L
1 mmol/L

PREPARATION
All the reagents are ready to use.
STORAGE AND STABILITY
All the components of the kit are stable until the expiration date on
the label when stored tightly closed at 2-8C, protected from light
and contaminations prevented during their use.
Do not use reagents over the expiration date.
Signs of reagent deterioration:
- Presence of particles and turbidity.
- Blank absorbance (A) at 520 nm
0.16.
ADDITIONAL EQUIPMENT
- SPIN640 Autoanalyzer.
- General laboratory equipment.
SAMPLES
- Serum or plasma1: Stability 3-5 days at 2-8C or 6 months at
20C.
- Urine (24 h)1: Stability 4 days at 15-25C, pH >8. Dilute sample
1/50 in distilled water. Mix. Multiply results by 50 (dilution factor);
If urine is cloudy; warm the specimen to 60C for 10 min to
dissolve precipitated urates and uric acid. Do not refrigerate.
BARCODED REAGENTS LOAD MUST BE PRECEDED OF A
SPINREACT DATABASE COPY INTO THE ANALYZER
SOFTWARE. IT IS AVAILABLE UNDER REQUEST TO
SPINREACT.

Prozone (Rate-Antigen)
PC
ABS

03/07/12

1
0

End Point
505

Direction
Reagent Blank
React. Time

Increase
15-16
76-77

-30000/30000

Lin. Range
Lin. Limit
Subs. Limit

0.03-25.00

Q1
Q2
Q3
Q4

The Calibration is stable until 36 days. After this period the Calibration
must be performed again in order to obtain good results.
QUALITY CONTROL
Control sera and calibrators are recommended to monitor the
performance of assay procedures: SPINTROL H Calibrator, SPINTROL
H Normal and Pathologic (Ref. 1002011, 1002120 and 1002210).
If control values are found outside the defined range, check the
instrument, reagents and technique for problems.
Each laboratory should establish its own Quality Control scheme and
corrective actions if controls do not meet the acceptable tolerances.
REFERENCE VALUES1
Serum or plasma:
Women 2.5 - 6.8 mg/dL
149 405 mol/L
Men
3.6 - 7.7 mg/dL
214 458 mol/L
Urine:
250 - 750 mg/24 h
1.49 - 4.5 mmol/24 h
These values are for orientation purpose; each laboratory should
establish its own reference range.
NOTES
1. Calibration with the aqueous standard may cause a systematic
error in automatic procedures. In these cases, it is recommended
to use a serum Calibrator.
2. Use clean disposable pipette tips for its dispensation.
BIBLIOGRAPHY
1.
2.
3.
4.
5.
6.

Schultz A. Uric acid. Kaplan A et al. Clin Chem The C.V. Mosby Co. St
Louis. Toronto. Princeton 1984; 1261-1266 and 418.
Fossati P et al. Clin Chem 1980;26:227-231.
Young DS. Effects of drugs on Clinical Lab. Tests, 4th ed AACC Press,
1995.
Young DS. Effects of disease on Clinical Lab. Tests, 4th ed AACC
2001.
Burtis A et al. Tietz Textbook of Clinical Chemistry, 3rd ed AACC 1999.
Tietz N W et al. Clinical Guide to Laboratory Tests, 3rd ed AACC 1995

PACKAGING
Ref. MD41001
Cont.
.

MDBSIS45-I

150
150

RESULT SETUP
Decimal
0.01 Slope
Unit
mg/dL Inter.

7.5

REACTION PARAMETERS
Reac. Type
Pri. Wave.
Sec. Wave.

Quinoneimine+ 4H 2O

REAGENT VOLUME
Vol. R1
Vol. R2
Vol. R3
Vol. R4

**
URIC
Uric Acid
1

R1: 3 x 40 mL
R2: 3 x 40 mL

SPINREACT,S.A./S.A.U Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN

Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail: spinreact@spinreact.com

URIC ACID -LQ

Acido rico
Uricasa -POD. Lquido
APLICACIN AL SPIN640

Determinacin cuantitativa de cido rico

IVD

TEST INFORMATION
N
Test
Full Name
Standard n

Conservar a 2-8C
PRINCIPIO DEL MTODO
El cido rico es oxidado por la uricasa a alantona y perxido de
hidrgeno (2H2 O2) que en presencia de peroxidasa (POD), 4aminofenazona (4-AF) y 2-4 Diclorofenol Sulfonato (DCPS)
forma un compuesto rosceo:
cido rico + 2H2O + O2
2H2 O2 + 4-AF + DCPS

Uricasa
POD

SAMPLE VOLUME
Vol. Sample Stand.
Vol. Sample Increas.
Vol. Sample Dec

Alantona + CO2 + 2H2 O2

La intensidad de quinonaimina roja formada es proporcional a la

concentracin de cido rico presente en la muestra ensayada1,2 .

JUDGEMENT CRITERIA
Absorbance
Incre.Test
Decre.Test

SIGNIFICADO CLNICO
El cido rico y sus sales son el producto final del metabolismo
de las purinas. En una insuficiencia renal progresiva hay una
retencin en sangre de urea, creatinina y cido rico.
Niveles altos de cido rico son indicativos de patologa renal y
generalmente se asocia con la gota1,5,6.
El diagnostico clnico debe realizarse teniendo en cuenta todos
los datos clnicos y de laboratorio.

50 mmol/L
4 mmol/L
60 U/L
660 U/L
200 U/L
1 mmol/L

PREPARACIN
Todos los reactivos estn listos para su uso.
CONSERVACIN Y ESTABILIDAD
Todos los componentes del kit son estables, hasta la fecha de
caducidad indicada en la etiqueta, cuando se mantienen los
frascos bien cerrados a 2-8C, protegidos de la luz y se evita su
contaminacin. No usar reactivos fuera de la fecha indicada.
Indicadores de deterioro de los reactivos:
- Presencia de partculas y turbidez.
- Absorbancia (A) del Blanco a 520 nm
0,16.
MATERIAL ADICIONAL
- Autoanalizador SPIN640
- Equipamiento habitual de laboratorio.
MUESTRAS
- Suero o plasma1: Estabilidad 3-5 das a 2-8C y 6 meses
a 20 C.
- Orina (24 h)1: Estabilidad 3 das a temperatura ambiente a pH
> 8. Diluir la muestra al 1/50 en agua destilada. Mezclar.
Multiplicar el resultado obtenido por 50 (factor de dilucin); Si
la muestra es turbia, calentarla a 60C 10 min. para disolver
los precipitados de urato y cido rico. No refrigerar.

Direction
Reagent Blank
React. Time

Increase
15-16
76-77

-30000/30000

Lin. Range
Lin. Limit
Subs. Limit

0.03-25.00

Q1
Q2
Q3
Q4

CONTROL DE CALIDAD
Es conveniente calibrar y analizar junto con las muestras sueros
control y calibradores valorados: SPINTROL H Calibrador, SPINTROL
H Normal y Patolgico (Ref. 1002011, 1002120 y 1002210).
Si los valores hallados se encuentran fuera del rango de tolerancia,
revisar el instrumento, los reactivos y el calibrador.
Cada laboratorio debe disponer su propio Control de Calidad y
establecer correcciones en el caso de que los controles no cumplan
con las tolerancias.
VALORES DE REFERENCIA1
Suero o plasma:
Mujeres
2,5 - 6,8 mg/dL
Hombres
3,6 - 7,7 mg/dL
Orina:
250 - 750 mg/24 h

149 405 mol/L

214 458 mol/L
1,49 - 4,5 mmol/24 h

Estos valores son orientativos. Es recomendable que cada laboratorio

establezca sus propios valores de referencia.
NOTAS
1. La calibracin con el Patrn acuoso puede dar lugar a errores
sistemticos en mtodos automticos. En este caso, se
recomienda utilizar calibradores sricos.
2. Usar puntas de pipeta desechables limpias para su dispensacin.
BIBLIOGRAFA
1.
2.
3.

5.
6.

Schultz A. Uric acid. Kaplan A et al. Clin Chem The C.V. Mosby Co. St
Louis. Toronto. Princeton 1984; 1261-1266 and 418.
Fossati P et al. Clin Chem 1980;26:227-231.
Young DS. Effects of drugs on Clinical Lab. Tests, 4th ed AACC Press,
1995.
Young DS. Effects of disease on Clinical Lab. Tests, 4th ed AACC
2001.
Burtis A et al. Tietz Textbook of Clinical Chemistry, 3rd ed AACC 1999.
Tietz N W et al. Clinical Guide to Laboratory Tests, 3rd ed AACC 1995.

Cont.
.

03/07/12

1
0

La Calibracin es estable hasta 36 das. Pasado este perodo es

necesario solicitar de nuevo la Calibracin para la obtencin de buenos
resultados.

PRESENTACIN
Ref. MD41001

MDBSIS45-E

150
150

End Point
505

Prozone (Rate-Antigen)
PC
ABS

4.

PARA LA CARGA DE REACTIVOS MEDIANTE EL CDIGO DE

BARRAS SE DEBE PRECARGAR LA BASE DE DATOS
DISPONIBLE BAJO SOLICITUD A SPINREACT.

REAGENT VOLUME
Vol. R1
Vol. R2
Vol. R3
Vol. R4

RESULT SETUP
Decimal
0.01 Slope
Unit
mg/dL Inter.

7.5

REACTION PARAMETERS
Reac. Type
Pri. Wave.
Sec. Wave.

Quinonaimina + 4H2 O

REACTIVOS
R1
Fosfatos pH 7,4
Tampn 2-4 Diclorofenol Sulfonato (DCPS)
Uricasa
R2
Peroxidasa (POD)
Enzimas Ascorbato oxidasa
4 - Aminofenazona (4-AF)

**
URIC
Uric Acid
1

R1: 3 x 40 mL
R2: 3 x 40 mL

SPINREACT,S.A./S.A.U Ctra.Santa Coloma, 7 E-17176 SANT ESTEVE DE BAS (GI) SPAIN

Tel. +34 972 69 08 00 Fax +34 972 69 00 99. e-mail: spinreact@spinreact.com