1.

MICROCOCCACEAE / STAPHYLOCOCCI
Gram positive cocci (commonly in clusters, grapelike)
Catalase + stomatoccoci (exception)
Motility
Consideration

2. STREPTOCOCCI
Gram positive cocci (commonly in pairs/tetrads)
Facultative anaerobes
Catalase Consideration
HEMOLYSIS & LANCEFIELD GROUPS, PYR, CAMP
3. GRAM NEGATIVE COCCI
Nieserria gonorrhea,meningitidis, catarrhalis
Kingella
Eikenella
Simonsiella
Alyiesella
4. ENTEROBACTERIACEAE
Gram negative
Motility (v) peritrichous flagella
Ferment ALL glucose rather oxidize glucose
Oxidase (-)
Catalase Nitrate - > nitrite
5. NONFERMENTATIVE GRAM NEGATIVE BACILLI- COCO BACILI
Pseudomonas
Burkholderia
Strenotrophomonas
Alcaligenes
Acinobacter
Eikenella

6. MYCOBACTERIA
Rod shaped
Slender
Slightly curved
NONMOTILE
Not produce SPORES
Acid fastness
CO2 LOVING
Grow slowly

1. Bile Esculin test

Enterococci vs. S. Bovis (group D)
Group D streptococci vs. non-group D viridians
P: Black
N:yellow
2. Decarboxylase test
Enterobacteriaceae vs. Gram (-) rods
Amino acid amine
P: alkaline (purple)
N: acid (yellow
Media: Peptic digest of animal tissue/bromcresol purple and red
Agar: brain heart infusion
3. DNase test agar
Serratia vs. Enterobacter
Staphylococcus from other specie
Moraxella catarrhalis from Neisseria spp.
DNA deoxyribonuclease
P: (7.5 ph colorless)
N: remain green (due to methyl green complex
Media: Pancreatic digest of casein, DNA acid, methyl green complex
Agar: DNase agar