Pentra XLR Daily Guide PDF

Hematology Analyzer
Daily Guide
Ref: RAB290CEN
Daily Guide
Daily Guide
Ref: RAB290CEN
Contents
1. Revisions..................................................................................................1
6. Running Blood Specimen................................................................15
2. Software Overview...............................................................................2
6.1. To Run the Specimen in Rack Mode............................................................ 15

6.2. To Run the Specimen in STAT Mode............................................................15
2.1. Contextual Toolbar Description..................................................................... 2

2.2. Function Toolbar Description........................................................................ 3
2.3. Main Menu Buttons...................................................................................... 4
2.4. Logs Menu Buttons......................................................................................4
2.5. Quality Assurance Menu Buttons...................................................................5
2.6. Service Menu Buttons.................................................................................. 6
2.7. Settings Menu Buttons................................................................................. 6
2.8. Cycle Selection Button................................................................................. 7
3. Start of Day............................................................................................. 8
3.1. To Check the Waste Container Level............................................................. 8
3.2. To Switch the Printer On .............................................................................. 8
3.3. Starting the Instrument................................................................................. 8
4. Running Quality Control Blood..................................................... 11

4.1. To Run a Control Blood in Rack Mode......................................................... 11
4.2. To Run a Control Blood in STAT Mode.........................................................11
4.3. To Check Control Blood Results..................................................................12
5. Worklist...................................................................................................13
5.1. To Create a New Order in Grid View............................................................ 13
5.2. To Create a New Order in Rack View........................................................... 13
5.3. To Search Orders.......................................................................................14
Daily Guide
Ref: RAB290CEN
7. Results Matching................................................................................17
7.1. Sample Identification..................................................................................17
7.2. To Manually Match Results......................................................................... 17
8. Results Management........................................................................ 18
8.1. To Filter Results......................................................................................... 18
8.2. To Customize Results.................................................................................18
8.3. To Validate Multiple Results........................................................................ 18
8.4. To Validate a Single Result..........................................................................19
8.5. To Reject Multiple Results...........................................................................19
8.6. To Reject a Single Result............................................................................ 19
8.7. To Rerun a Sample Manually.......................................................................20
8.8. To Print your Results.................................................................................. 20
8.9. To Send Results to the LIS..........................................................................20
9. Results Interpretation.......................................................................22
9.1. General Alarms.......................................................................................... 22
9.2. Morphology Alarms.................................................................................... 24
9.3. Suspected Pathologies...............................................................................29
9.4. Analyzer Alarms......................................................................................... 31
9.5. Statistical Function Alarms..........................................................................32
9.6. Delta Check...............................................................................................32
10. Archives............................................................................................... 34
10.1. To View Archived Results..........................................................................34
10.2. To View Exported Results......................................................................... 34
10.3. To Print Archived Results.......................................................................... 34
10.4. To Send Archived Results to the LIS.......................................................... 35
10.5. To Delete Archived Results....................................................................... 35
11. End of Day...........................................................................................36

11.1. Statistics................................................................................................. 36
11.2. To Change Operator.................................................................................36
11.3. Stopping the Instrument........................................................................... 37
Daily Guide
Ref: RAB290CEN
ii
Revisions
1. Revisions
Index
Reference
Software Version
Document Date Issued
RAB290AEN
2.0.x
June 2013
RAB290BEN
2.0.x
November 2013
RAB290CEN
2.2.x
April 2014
This document applies to the latest software version listed and higher versions.
When a subsequent software version changes the information in this document, a new
electronic edition (DVD-ROM and/or online help) is released and supplied by
HORIBA Medical.
To update a paper document, please contact your local HORIBA Medical representative.
This instrument is intended for professional use only.
The user manual is supplied on the DVD-ROM and must be consulted prior
to operating the instrument.
Please refer to the user manual for potential hazards and risks, technical
specifications and performance data.
This document is only a guide for daily usual operations and cannot
replace the instrument user manual.
If a problem occurs or an alarm is displayed during the instrument operation,

refer to the user manual instructions for recommendations. If the problem
persists, please contact your local HORIBA Medical representative.
Documentation DVD-ROM instructions

To view or to print the user manual or any other document included in the
Documentation DVD-ROM, place it into a computer drive and follow the instructions.
YOUR LOCAL REPRESENTATIVE:
Daily Guide
Ref: RAB290CEN
Software Overview
Contextual Toolbar Description
2. Software Overview
The Pentra XLR includes a software application that allows you to navigate in the various
menus. The touch screen allows easy and direct access to all functions via icons.
Press the icon or use the external mouse to activate corresponding functions.
2.1. Contextual Toolbar Description

Depending on the screen currently displayed, buttons of the contextual toolbar may
change. Buttons listed below are the most commonly displayed:
Help: displays the help.
Print/Send: allows to print data.
The main menu includes the following items:

The contextual toolbar (horizontal), which provides functionalities related to the
screen currently displayed.
The function toolbar (vertical), which provides a direct access to other
functionalities.
The main menu buttons (center of the screen), to enter the submenus.
StartUp to perform a Startup cycle manually.
ShutDown to perform a Shutdown cycle manually.
The status bar (bottom of the screen), which gives indication on the date, time,
software version, operator code and the cycle in progress.
Daily Guide
Ref: RAB290CEN
Detail: displays more details regarding the current screen.
Insert: inserts new data.
Software Overview
Function Toolbar Description
Delete: deletes an item or data.
2.2. Function Toolbar Description
STOP: stops the instrument (emergency stop).

Edit: allows to modify data.
Alarm: displays the list of alarms.

Validate: validates an action.
Worklist: opens the worklist.

Cancel: cancels an action.
Start Rack: runs an automatic sampling.

Return: goes back to the previous screen.
STAT Mode: runs a manual sampling.

Quit: quits the application.
Report: opens the list of reports.
Archive: opens the archived results.
Daily Guide
Ref: RAB290CEN
Software Overview
Main Menu Buttons
2.3. Main Menu Buttons
Service: opens the service menu.
StartUp: launches a Startup cycle.

Settings: displays the settings screen.
ShutDown: launches a Shutdown cycle.
Logs: opens the instrument logs menu.
2.4. Logs Menu Buttons

Quality Assurance: gives access to QC, XB, calibration and repeatability.
Maintenance: displays the list of maintenance procedures performed.
Run in Progress: displays a list of the samples currently running.
Data Handling: displays logs related to patients.
Status: displays the reagents monitoring screen.
Host: displays events related to sent or printed data.
Errors: displays the list of all the errors that occurred on the instrument.
Daily Guide
Ref: RAB290CEN
Software Overview
Quality Assurance Menu Buttons
Blank Cycle: displays blank cycle results.
2.5. Quality Assurance Menu Buttons
XB: displays the XB graphs.

Calibration: displays events related to calibration.
Quality Control: displays active blood controls and gives access to QC
Management screen.
Settings: displays settings performed on the instrument.
Within Run: allows you to perform a repeatability test on the instrument.

Quality Control: displays events related to quality assurance.
Calibration: allows you to calibrate the instrument.

Reagents: displays events related to reagents.
Logs per Day: displays all the events that occurred at a given date.
Daily Guide
Ref: RAB290CEN
Software Overview
Service Menu Buttons
2.6. Service Menu Buttons
Only certified HORIBA Medical technicians have access to the Technician

Menu. The button will be grayed out for any other user.
Miniclean: launches a short rinsing cycle of the counting chambers.
Concentrated Cleaning: starts a concentrated cleaning procedure using

ABX Minoclair.
2.7. Settings Menu Buttons
AutoClean: launches a cleaning cycle using ABX Cleaner.
Rules: allows to define rules for printing, sending to host and for rerun
conditions.
Super User Menu: gives access to customer maintenance.
System: allows to set various system options such as date and time or
printer options.
Technician Menu: (reserved to technicians) gives access to advanced

maintenance.
Save/Restore: allows to save and restore the software configuration.
Users: allows to define operator profiles.

The head of the laboratory designates who the Super User is.
Super User is made of three additional menus:
Mechanical System which allows the management of mechanical cycles.
Hydraulic System which allows the management of hydraulic cycles.
Others which facilitates certain maintenance procedures, and allows to force
calibration coefficient values and RET parameters.
Daily Guide
Ref: RAB290CEN
Type Parameter Setting: allows to configure the instrument according to

the blood type run.
Software Overview
Cycle Selection Button
Soft Parameters: allows to edit software options.
Quality Assurance: allows to define CV for calibration, QC and

repeatability. The XB mode is also set here.
2.8. Cycle Selection Button

CBC/DIFF/RET: allows you to choose the test you want to perform
To perform a RET analysis, you need to activate the RETIC box and then select RET
by pressing CBC/DIFF/RET.
Daily Guide
Ref: RAB290CEN
Start of Day
To Check the Waste Container Level
3. Start of Day
Check the alignment of the paper if the printer used is a tractor feed printer.
3.1. To Check the Waste Container Level
Press the ON/OFF switch.
1. Check the level of waste in the container.

2. If it needs to be emptied, refer to the Maintenance and Troubleshooting >
Replacement Procedures > To Replace Reagents > To Replace the Waste Container
chapter of the user manual.
When disposing of waste, protective clothing must be worn (lab coat, gloves,
eye protection, etc.). Follow your local and/or national guidelines for biohazard
waste disposal.
At the beginning of each day, before startup, check if the waste container
needs to be emptied.
During instrument operation, do not remove the reagent tubes and the
liquid waste tube under any circumstance.
Waste must be handled according to your local and/or national regulations.
3.3. Starting the Instrument
3.3.1.
To Switch the Instrument On
1. Switch the instrument on.

2. Wait during initialization.
3. Log in to the application.
Refer to the Start of Day > Starting the Instrument > To Log In to the Application
chapter.
3.3.2.
To Log In to the Application
3.2. To Switch the Printer On

1. Select a user name.
2. Enter your password.
Make sure that the printer has enough paper for daily operations. If not, add some paper
following the instructions of the printer user guide.
Daily Guide
Ref: RAB290CEN
3. (Optional) Select Disable Auto Loader if you want to work in STAT mode only.
Start of Day
Starting the Instrument
4. Select the following options:

Erase Worklist
Reset AutoNumbering
Archive Reports
This step can only be performed when you start the instrument for the first
time of the day.
The first two options are optional, depending on your laboratory's rate of
analysis. It is highly recommended to select the third option at the first
startup of each day.
3.3.4.
Instrument Startup
Startup is used to control the analyzer before running analysis. It can be launched
manually, or automatically depending on the application settings.
3.3.4.1. To Perform a Manual Startup

Access: Main Menu > StartUp
5. Press OK.
1. Press StartUp.
3.3.3.
2. Select which cycle you want to perform:
To Control the Reagents

Access: Main Menu > Status
The system can manage HORIBA Medical reagents automatically (levels and expiration
date). It informs the user about the reagents status at the end of the instrument start, or
displays an alarm message in the Status screen if a reagent runs low or has expired.
However, it is recommended to check the reagent levels and expiration date before
starting the system. To do so, you need to:
1. Check the level of the reagent bottles from the software.
2. Visually check the lot number and expiration date on the reagent bottles.
3. If a reagent bottle has to be changed, refer to the Maintenance and Troubleshooting
> Replacement Procedures > To Replace Reagents chapter of the user manual.
Daily Guide
Ref: RAB290CEN
A RETIC BLANK CYCLE must be performed before the first RET analysis of each
day.
3. Wait until the cycle is over.
Either cycle takes about 3 minutes 30 seconds.
Blank cycles (cycles without any blood specimen) are performed during the startup
cycle. The startup passes if the background counts are within acceptable limits:
Parameter
Background count limits
WBC
0.3 x 103/mm3
RBC
0.03 x 106/mm3
HGB
0.3 g/dL
PLT
7 x 103/mm3
Start of Day
Starting the Instrument
Parameter
Background count limits
LMNE
< 0.30#
RET
< 300#
Results of the startup are saved and can be consulted in the Blank Cycle logs.
It is possible to run analyses after a failed startup cycle but a "Startup Failed"
message is displayed on the following cycles. Refer to the Maintenance and
Troubleshooting > Troubleshooting Procedures > Operation Problems >
Startup Failed chapter of the user manual for more information.
If the system is not used for a period superior to 36 hours, it is mandatory to

power it down. This eliminates startup problems, as well as the possibility of
the dilution chambers evaporating.
3.3.4.2. To Schedule an Automatic Startup

Access: Main Menu > Settings > System > Cycle Option
When you schedule an Automatic Startup, it is run as soon as connections with the
instrument and reagents level have been checked.
1. Press Edit in the contextual toolbar.
2. In the StartUp area, select Automatic.
3. In the Begin the day area, specify the startup time.
4. Press Validate in the contextual toolbar.

Results of the startup are saved and can be consulted in the Blank Cycle logs.
Daily Guide
Ref: RAB290CEN
10
Running Quality Control Blood

To Run a Control Blood in Rack Mode
4. Running Quality Control Blood

4.2. To Run a Control Blood in STAT Mode
Before analyzing any patient blood samples, it is recommended to perform
quality control analysis on three levels of control blood material (low,
normal and high) to make sure that the instrument is performing within the
specified ranges of the quality control material.
Check control status in the Quality Control tab. If necessary, create a
control. Refer to the Quality assurance > Quality Control chapter of the
user manual.
It is recommended to run control bloods daily to check the performance of the

instrument. Make sure you created the control lot before running it so that it is
not run like a blood sample.
RET controls can only be run in STAT mode.
To run a control blood in STAT mode, you need to:
4.1. To Run a Control Blood in Rack Mode
1. Prepare your control blood according to the specific instructions detailed in the
control blood leaflet (temperature, mixing, etc.).
2. Press STAT Mode to open the tube holder door.
3. Enter the lot number either manually or using the external barcode reader.
It is recommended to run control bloods daily to check the performance of the

instrument. Make sure you created the control lot before running it so that it is
not run like a blood sample.
RET controls can only be run in STAT mode.
To run a control blood in Rack mode, you need to:
1. Prepare your control blood according to the specific instructions detailed in the
control blood leaflet (temperature, mixing, etc.).
2. Place the tube on a rack.
Make sure that the barcode label is visible for the internal barcode reader.
3. Place the rack on the rack loader.
4. Press Start Rack.

5. Gently mix the control blood.
6. Put the tube in the tube holder and close the door to start sampling.
When placing your tube in the tube holder, make sure that you are using
the appropriate position and that the cap can be pierced. If not, remove
the cap before closing the door.
7. When the door opens, remove the tube from the tube holder and recap it if needed.
When the analysis is complete, the QC results are automatically saved in Quality
Assurance > Quality Control.
When the analysis is complete, the QC results are automatically saved in Quality
Assurance > Quality Control.
Daily Guide
Ref: RAB290CEN
11
Running Quality Control Blood

To Check Control Blood Results
4.3. To Check Control Blood Results

Access: Main Menu > Quality Assurance > Quality Control
1. Select a control lot.

2. Press Detail in the contextual toolbar.
3. Check that the results are within control target values range.
4. If results are out of range, perform a concentrated cleaning and rerun the control
blood.
Refer to the Maintenance and Troubleshooting > Maintenance > Hydraulic
Maintenance > To Perform a Concentrated Cleaning chapter of the user manual.
If results are still out of range, please contact your local HORIBA Medical representative.
Daily Guide
Ref: RAB290CEN
12
Worklist
To Create a New Order in Grid View
5. Worklist
3. Select the test to perform by pressing CBC/DIFF/RET.
5.1. To Create a New Order in Grid View
To perform a RET analysis, you need to activate the RETIC box and then
select RET by pressing CBC/DIFF/RET.
Access: Main Menu > Worklist
1. Press Insert in the contextual toolbar.

2. Enter all the information related to the blood sample and to the patient.
If you do not select the test that needs to be performed, the default test
will be performed.
5.2. To Create a New Order in Rack View

1. Press Rack View to display the rack view.

2. Enter the rack number using the external barcode reader.
3. Select an empty position to enter a new sample tube.
If the tube position is not empty, both sample and patient information is displayed.
Daily Guide
Ref: RAB290CEN
13
Worklist
To Search Orders
4. Enter all the information related to the blood sample and to the patient.
5.3. To Search Orders
5.3.1.
To Search a Sample
From the worklist, you can look for a specific sample record. To do so, you need to:
2. Press Search Sample.
3. Type the first characters of the sample ID in Sample ID.
4. When you have located the right sample, select it and press Validate.
5.3.2.
To Search a Patient Name

You cannot create an order for a RET analysis in Rack mode.
From the worklist, you can look for a specific patient file. To do so, you need to:
2. Press Search Patient.
will be performed.
3. Type the first letters of the patient name in Patient Name.

4. When you have located the right sample, select it and press Validate.

The cursor automatically moves to the next rack position. If there is no more position
available for the current rack, the cursor moves to the next rack number.
Daily Guide
Ref: RAB290CEN
14
Running Blood Specimen

To Run the Specimen in Rack Mode
6. Running Blood Specimen

6.2. To Run the Specimen in STAT Mode
6.1. To Run the Specimen in Rack Mode
RET analyses can only be performed in STAT mode.
RET analyses can only be performed in STAT mode.

A Startup RET cycle is automatically performed before the first RET analysis of
the day.
A blood specimen can be run either in Rack mode or in STAT mode. To run it in STAT
mode, you need to:
A blood specimen can be run either in Rack mode or in STAT mode. To run it in Rack
mode, you need to:
1. Press STAT Mode to open the tube holder door.
1. Prepare your worklist.
2. If you work with tubes with barcode labels, place the tube on any rack, at any
position.
Make sure that the barcode label is visible for the internal barcode reader.
3. If you work with tubes with no barcode labels, place the tube on the rack and at the
position specified in the worklist.
4. Place the rack on the rack loader.
2. Enter information about the sample.
will be performed.
4. Press CDR if you need a post-dilution on the sample.
5. Press Start Rack.

The STAT mode can be used while analyses in Rack mode are already
running. In this case, the analyses on the rack tubes are paused, and the
STAT specimen is run in priority.
It is not possible to use the CDR mode on RET samples.
Daily Guide
Ref: RAB290CEN
15
Running Blood Specimen

To Run the Specimen in STAT Mode
5. Select the appropriate dilution ratio for WBC/LMNE and for RBC/PLT/HGB.

7. Gently mix the blood specimen.
8. Put the tube in the tube holder and close the door to start sampling.
When placing your tube in the tube holder, make sure that you are using
the appropriate position and that the cap can be pierced. If not, remove
the cap before closing the door.
A bargraph is displayed in the status bar so that you can see the progress of the
analysis.
9. When the door opens, remove the tube from the tube holder and recap it if needed.
The STAT mode can be used while analyses in Rack mode are already
running. In this case, the analyses on the rack tubes are paused, and the
STAT specimen is run in priority.
The software automatically leaves the STAT Mode screen after 10 minutes of
inactivity. If a pop-up window is displayed at the time, the software cannot
leave the screen. Make sure you treat software required actions as soon as
possible to prevent such a problem.
Daily Guide
Ref: RAB290CEN
16
Results Matching
Sample Identification
7. Results Matching
You can also validate results that are not associated to an order. In this case,
they will be matched to the default order (default test).
7.1. Sample Identification

A sample can be identified in one of two ways:
through Barcode identification
through Rack/Position identification
Identifying a sample is what allows the instrument to automatically match an order to a
sample, or the operator to manually match an order to a sample.
7.2. To Manually Match Results

Access: Main Menu > Reports > Match View
To match results and orders, you need to:

1. Select an unmatched order in the top area.
2. Select an unmatched result in the bottom area.
A pop-up window prompts you to confirm.
4. Press OK.
The order and the result are matched and can now be consulted in the Reports List.
If the sample type is different for the order and the result, the result will be recomputed.
It will be flagged in the Remarks field.
Daily Guide
Ref: RAB290CEN
17
Results Management
To Filter Results
8. Results Management
RET results are automatically validated and cannot be customized or edited in
any way.
8.1. To Filter Results

1. Select the result you want to customize to open the Report Details screen.
Access: Main Menu > Reports
In the Report List, you can filter the results in order to display only the results you need.
To do so, you need to:
1. Select one of the following options:
Unvalidated
Rejected
Validated and Transmitted
Validated but not Transmitted
Waiting for examination
2. Select the results you want to review.
8.2. To Customize Results
2. Review the results of each run using the left or right arrows.
Compare the values of each run to the history values (if available).
3. Select or deselect the groups of parameters you want to show or hide for each run to
create a composite report.
The composite result is displayed in the Reports area.
If a parameter group including alarms is selected, these alarms are associated to the
composite result.
4. If you want to replace individual values manually, press Edit in the contextual toolbar
to display the Report Edit screen.
5. Replace the values that need to be replaced.
6. Deselect Waiting for Examination after reviewing the result.
8.3. To Validate Multiple Results

You can customize unvalidated results to:
hide groups of parameters (RBC, PLT, WBC or DIFF),

replace individual values manually,
add comments about the patient, the sample or suspected pathologies,
create a composite of the different runs of a sample.
Daily Guide
Ref: RAB290CEN
Unvalidated results can be manually validated. To do so, you need to:

1. Select Unvalidated.
2. Select the results you want to validate.
18
Results Management
To Validate a Single Result
3. Press Validate selected reports.
8.5. To Reject Multiple Results


4. Press OK.
Unvalidated results can be manually rejected. To do so, you need to:

Selected results are validated.
2. Select the results you want to reject.

3. Press Reject selected reports.
8.4. To Validate a Single Result

Unvalidated results can be manually validated. To do so, you need to:
4. Press OK.
Selected results are rejected.
2. Click the result you want to validate to open the Report View screen.
3. Press Validate selected reports.
8.6. To Reject a Single Result

4. Press OK.
The selected result is validated.
Unvalidated results can be manually rejected. To do so, you need to:

2. Click the result you want to reject to open the Report View screen.
3. Press Reject selected reports.
Daily Guide
Ref: RAB290CEN
19
Results Management
To Rerun a Sample Manually
4. Press OK.
2. Press Print/Send in the contextual toolbar.
The selected result is rejected.
8.7. To Rerun a Sample Manually


Print report list for selected rows
Print compact report list for selected rows
Print report list for all rows
Print compact report list for all rows
Print report in full page for selected rows
Print report in full page for all rows
Print the last report in full page
Print the run + raws in full page for selected rows
4. Press OK.
You cannot rerun a sample if it has no matched results.
You can rerun a sample manually. To do so, you need to:
You can only print the raw counts if the option has been enabled in Main
Menu > Settings.
Refer to the Settings > System > Printer Tab > To Enable Specific Data
Printing chapter of the user manual for more information.
1. Select the result of the sample you want to rerun to open the Report View screen.
2. Press Rerun.
8.9. To Send Results to the LIS

3. Press OK.
A new order is created in the worklist.
8.8. To Print your Results

1. Select the results you want to print.
Daily Guide
Ref: RAB290CEN
After each analysis cycle, validated results are automatically sent to the LIS if
the option has been enabled.
Refer to the Settings > Rules > Print/Transmit Conditions Tab > To Configure
Transmit Conditions chapter of the user manual for more information.
1. Select the results you want to send to the LIS.
3. Select the Send tab.
20
Results Management
To Send Results to the LIS

Send the last report
Send the Selected Reports
Send all the Reports
5. Press OK.
Daily Guide
Ref: RAB290CEN
21
Results Interpretation
General Alarms
9. Results Interpretation
Results that exceed the Panic limits are identified with an alarm:
9.1. General Alarms
L for results below the panic lower limit

H for results above the panic upper limit
If an L alarm or an H alarm is triggered, you must be particularly careful when
analyzing the results. Make sure you check the anteriority of the patient and
rerun the sample if there is no clinical specificity.
9.1.1.
Parameter Reject
Reject flags occur whenever the difference between the two counts of a parameter is
higher than the predefined limits. They indicate that the results for the flagged
parameters are inconclusive, and that they should be investigated for the manual rerun
status, and/or an instrument malfunction if the flag occurs on every sample. Rejected
results are followed by an "*".
Samples which trigger a reject cannot be validated and should be rerun.
9.1.2.
Results Exceeding Linearity Range and Visible Range
Parameter
Linearity Limits
Visible Range
> Visible Range
WBC
result
"result+D" displayed and

printed
"result+O" transmitted to the
LIS*
"result+X" transmitted to the
LIS**
"--- D" displayed and printed

"--- O" transmitted to the
LIS*
"--- X" transmitted to the
LIS**
Suspicion
Results are followed by an "!" when the analyzer detects a possible anomaly during the
count. The sample must be rerun.
9.1.3.
9.1.4.
LYM, MON, NEU, EOS, BASO, ALY and LIC replaced by

"---"
RBC
Normal and Panic Ranges
Results that exceed the Normal limits are identified with an alarm:
l for results below the normal lower limit
h for results above the normal upper limit
Daily Guide
Ref: RAB290CEN
result

printed
LIS*
LIS**

LIS*
LIS**
MCV, MCH, MCHC and RDW replaced by "---"

*:
ABX format
ASTM format
***: "Platelet Concentrate Mode" is displayed and printed when HGB < 2g/dL and PLT > 15 x
103/mm3
**:
22
General Alarms
Parameter
Linearity Limits
Visible Range
> Visible Range
HGB
result

printed
LIS*
LIS**

LIS*
LIS**
MCH and MCHC replaced by "---"

HCT
result

printed
LIS*
LIS**

LIS*
LIS**
MCH and MCHC replaced by "---"

PLT
result

printed***
LIS*
LIS**
"--- D" displayed and

printed***
LIS*
LIS**
MPV, PCT and PDW replaced by "---"

RET%
result

printed
LIS*
LIS**

LIS*
LIS**
CDR Mode
Parameter
Dilution Ratio Value
Dilution Ratio Value Visible

Range
WBC
"value+D" displayed and printed

"value+O" transmitted to the LIS*
"value+X" transmitted to the LIS**

"--- O" transmitted to the LIS*
"--- X" transmitted to the LIS**
RBC


HGB


HCT


PLT


*:
**:
ABX format
ASTM format
It is not possible to use the CDR mode on RET samples.
*:
ABX format
ASTM format
***: "Platelet Concentrate Mode" is displayed and printed when HGB < 2g/dL and PLT > 15 x
103/mm3
**:
Daily Guide
Ref: RAB290CEN
Use ABX Diluent to dilute the sample when a "--- D" alarm occurs on a
parameter.
23
Morphology Alarms
9.2. Morphology Alarms
9.2.2.
Platelet Alarms
9.2.2.1. MICP Alarm
9.2.1.
This alarm indicates the

presence of microcytes in the
platelets counting area. If
associated with a PLT reject "*",
PLT results are not reliable.
RBC Alarms
RBC1 and RBC2 thresholds define the microcytic and macrocytic thresholds. They are
calculated according to the MCV and the RDW. Microcytes are RBCs located before the
RBC1 threshold and macrocytes are RBCs located after the RBC2 threshold.
Microcytes values for Standard type are 5%, and Macrocytes values for Standard
type are 45%.
Flags are generated when results go beyond these values.
Flag
Triggering conditions
MIC
MIC% > 5%
MIC+
MIC% > 10%
MIC++
MIC% > 15%
MAC
45 < MAC% <= 98
MAC+
98 < MAC% <= 99
MAC++
99 < MAC%
Daily Guide
Ref: RAB290CEN
9.2.2.2. SCH Alarm

It indicates the presence of
schistocytes in the platelets
counting area. The result should
be further investigated.
If platelet aggregates or platelet clumping are suspected, the patient sample

should be redrawn in a sodium citrate tube. Do not vortex the sample.
24
Morphology Alarms
9.2.2.3. SCL Alarm
9.2.4.
LMNE Alarms
The SCL (Small Cell) alarm

indicates the presence of small
cells in the 2 fL and 3 fL zone. A
second analysis should be
carried out and the results
verified.
9.2.4.1. ALY Alarm

Atypical LYmphocytes
Suspected abnormalities
9.2.3.
WBC/BAS Alarms
Large lymphocytes
Reactive lymphoid forms
Stimulated lymphocytes
Plasmocytes
ALY values for Standard type are 2.5% and

0.25#
L1 Alarm
The L1 alarm can be triggered both on CBC
mode and on DIFF mode.
Suspected abnormalities:
PLT aggregates
Erythroblasts
9.2.4.2. LIC Alarm

Large Immature Cells
Promonocytes
Monoblasts
Metamyelocytes
Myelocytes
Promyelocytes
Myeloblasts
Blasts
Large lymphocytes
Prolymphocytes
LIC values for Standard type are 3% and 0.3#
Daily Guide
Ref: RAB290CEN
25
Morphology Alarms
9.2.4.3. LL Alarm
9.2.4.5. LN Alarm
Left Lymphocytes
Left Neutrophils
Platelet aggregates
Small lymphocytes
Erythrocyte membrane resistant to lysis
(stroma)
Erythroblasts
LL values for Standard type are 3% and 100#
Neutrophil destruction due to incorrect

storage of the sample or old sample
Contamination, stroma or platelet
aggregates
(stroma)
LN values for Standard type are 1.8% and 60#
9.2.4.4. LL1 Alarm
9.2.4.6. RN Alarm
Left Lymphocytes 1
Right Neutrophils
Platelet aggregates
Small abnormal lymphocytes
(stroma)
Erythroblasts
LL1 values for Standard type are 5% and 55#
Daily Guide
Ref: RAB290CEN
Large neutrophils and band cells

Immature cells from granulocytic line
(metamyelocytes, myelocytes,
promyelocytes, myeloblasts)
RN values for Standard type are 0.5% and

40#
26
Morphology Alarms
9.2.4.7. RM Alarm
9.2.4.9. MN Alarm
Right Monocytes
Monocytes / Neutrophils
Large monocytes
Hyperbasophilic monocytes
Myelocytes or promyelocytes
Blasts
Hypergranulation of monocytes or
hyperbasophilic monocytes
Young neutrophils with non-segmented
nuclei (bandcells)
RM values for Standard type are 0.7% and

60#
MN values for Standard type are 1.2% and

100#
9.2.4.8. NL Alarm
9.2.4.10. NE Alarm
Neutrophils / Lymphocytes
Neutrophils / Eosinophils
Small neutrophils without granules and/or

slightly segmented
Lymphocytes with a segmented nucleus or
activated lymphocytes
Neutrophils with membrane weakness
NL values for Standard type are 2% and 120#
Young eosinophils
Giant hypersegmented neutrophils
Eosinophils with low intracytoplasmic
material
Immature cells
Neutrophils with cytotoxic granulations
NE values for Standard type are 1.1% and 30#
Daily Guide
Ref: RAB290CEN
27
Morphology Alarms
9.2.4.11. NO Alarm
Background Noise
9.2.5.
Reticulocyte Alarms
9.2.5.1. PIT Alarm
Platelet aggregates
Large number of platelets
(stroma)
Erythroblasts
Background noise
NO values for Standard type are 2% and 80#
Platelet interference
Macro platelets
Platelet aggregates
Old sample
PIT values for Standard type are 20% and 40#
Daily Guide
Ref: RAB290CEN
28
Suspected Pathologies
9.2.5.2. FIT Alarm
9.3. Suspected Pathologies
Fluorescence interference
If the mobile threshold F1 cannot be positioned
between mature red blood cells and
reticulocytes, the FIT alarm is generated.
9.3.1.
WBC Messages
Suspected abnormalities:
Blockage or air bubbles into flowcell

Poorly aligned flowcell
Poorly adjusted sigma factor
Poorly adjusted optical gain
9.2.5.3. NRBC Alarm
Erythroblasts
Erythroblasts
NRBC value for Standard type is 1.0%
* PDW, PCT, ALY#, ALY%, LIC#, LIC%, RETL, RETM, RETH, MRV and RHCc
have not been established as indications for use in United States for this
instrument. Their use should be restricted to Research Use Only (RUO). Not
for use in diagnostic procedure.
Message
Triggered if...
Leukocytosis
WBC > WBC H
Leukopenia
WBC < WBC L
Lymphocytosis
LYM# > LYM# H or if LYM% > LYM% H
Lymphopenia
LYM# < LYM# L or if LYM% < LYM% L
Neutrophilia
NEU# > NEU# H or if NEU% > NEU% H
Neutropenia
NEU# < NEU# L or if NEU% < NEU% L
Eosinophilia
EOS# > EOS# H or if EOS% > EOS% H
Myelemia
NEU% > NEU% H and LIC# > LIC# H
LIC
LIC# > LIC# H or if LIC% > LIC% H
ALY
ALY# > ALY# H or if ALY% > ALY% H
Left Shift
(MN or NL) and RN
Monocytosis
MON# > MON# H or if MON% > MON% H
Basophilia
BAS# > BAS# H or if BAS% > BAS% H
Pancytopenia
WBC < WBC L and RBC < RBC L and PLT < PLT L
Blasts
BAS# > BAS# H and LIC# > LIC# H and RM
WBC Interpretation Not Possible

WBC < 0.1x103/mm3 or WBC > 85.0x103/mm3 or CO alarm
Daily Guide
Ref: RAB290CEN
29
Suspected Pathologies
9.3.2.
RBC Messages
Message
Triggered if...
Anemia
HGB < HGB L
Anisocytosis
RDW > RDW H
Microcytes
MIC Alarm
Microcytes+
MIC% > 10%
Microcytes++
MIC% > 15%
Macrocytes
MAC Alarm
Hypochromia
MCHC < MCHC l
Cold Agglutinin
MCHC > MCHC H and WBC < 91.3x103/mm3
Microcytosis
MCV < MCV L
Macrocytosis
MCV > MCV H
Erythrocytosis
RBC > RBC H
RBC Interpretation Not Possible

RBC < 0.01x106/mm3 or RBC reject (or RBC > 0.03 during Startup)
Message
Triggered if...
Platelet Aggregates
Condition 1:
PLT < 150x103/mm3 + WBC reject, or

NO + PDW > 20, or
NO + MPV > 10, or
NO + PLT < 150x103/mm3, or
NO + WBC reject, or
(L1 or LL1) + PDW > 20, or
(L1 or LL1) + MPV > 10, or
(L1 or LL1) + PLT < 150x103/mm3, or
PDW > 20 + PLT < 120x103/mm3 (in CBC mode only, a
suspicion "!" is triggered on PLT)
Condition 2:
NRBCs
LL, or
WBC reject + L1, or
WBC reject + LL1
Platelet Aggregates
NRBCs
If conditions 1 and 2 are not satisfied

and if L1 or LL1 or WBC reject
Macroplatelets
MPV > 11
PLT Interpretation Not Possible

PLT < 5.0x103/mm3 or PLT reject (or SCL alarm during Startup)
9.3.3.
PLT Messages
9.3.4.
RET Messages
Message
Triggered if...
Thrombocytosis
PLT > PLT H
Thrombocytopenia
PLT < PLT L
Message
Triggered if...
Microcytosis
MICP Alarm
Reticulocytosis
RET# > RET# H and RET% > RET% H
Schizocytes
No threshold between RBC and PLT on the curves.
Reticulopenia
RET# < RET# L and RET% < RET% L
Small cells
Small cells at the beginning of the platelet curve.
Daily Guide
Ref: RAB290CEN
30
Analyzer Alarms
9.4. Analyzer Alarms
9.4.1.
NO Alarm
More about the NO alarm in the Results Interpretation > Morphology Alarms > LMNE
Alarms chapter.
9.4.2.
MB Alarm
Meaning
Monocytes / Basophils
Conditions
The percentage of basophils found in the basophil channel is

above the percentage of Lympho / Mono / Neutro raw counts
found on the matrix channel.
Possible
triggers
Small basophils in the ALY area

Blasts
Contamination of the basophil channel
CO Alarm
Meaning
Poor correlation
Conditions
The percentage of validated cells is abnormally low: correlation

between resistive and optical measurements is < 50%
9.4.3.
9.4.4.
BASO+ Alarm
Conditions
The BASO+ alarm occurs on DIFF mode only.

BAS% > 50%
Consequences
The basophils are not taken away from the matrix populations, and
BAS% and BAS# are replaced by "---"
Daily Guide
Ref: RAB290CEN
9.4.5.
WBC / LMNE / BAS Balance
If the difference between the LMNE and WBC / BAS counts is higher than the defined
threshold, depending on the quantity of cells measured, a LMNE+ or a LMNE- alarm is
generated.
9.4.6.
RBC / RET Balance
If the difference between the two counts is higher than the defined threshold, a
BALANCE_RET+ or a BALANCE_RET- alarm is generated.
31
Statistical Function Alarms
9.4.7.
Reticulocyte Alarms
You can:
Alarm
Triggered if...
Consequence
LOW_COUNT
Number of counted particles on the

RET matrix < 10000 (absolute value)
"!" on all the reticulocyte parameters
LASER
Laser power output different from the

one specified on your instrument
All the reticulocyte parameters are

replaced by "----"
set the QC Failed alarm as a blocking alarm (refer to the Settings > Soft Parameters >
General Tab > To Set Blocking Alarms chapter of the user manual for more
information),
ensure the QC Failed alarm appears when you print results (refer to the Settings >
Soft Parameters > General Tab > To Set Blocking Alarms chapter of the user manual
for more information).
RBC alarms can also be triggered in RET mode. They are linked to the RBC
morphology.
9.6. Delta Check

9.5. Statistical Function Alarms
9.5.1.
XB Alarm
The XB alarm is associated with patient quality control. It is triggered when:

the XB alarm is enabled (refer to the Settings > Quality Assurance > To Configure the
XB Alarm chapter of the user manual for more information),
one of the mean values in a batch of 20 samples is outside the limits (refer to the
Quality Assurance > Patient Quality Control (XB) > To Modify XB Limits chapter of the
user manual for more information).
9.6.1.
Delta Check Overview
When you run an analysis, the delta check function checks the history of the patient
(when available) and compares the values of the previous analyses to those of the
current analysis for each parameter. The delta check is the coefficient of variation
between the previous analyses and the current one. There are two different values for
the delta check:
the absolute delta check,
the relative delta check.
An XB alarm can stop the instrument if you have set it as a blocking alarm (refer to the
Settings > Soft Parameters > General Tab > To Set Blocking Alarms chapter of the user
manual for more information).
9.6.2.
9.5.2.
QC Failed
The QC Failed alarm is triggered when the quality control results are out of range.
Daily Guide
Ref: RAB290CEN
Absolute Delta Check
The absolute delta check refers to the absolute difference between the current analysis
and the previous analyses.
D = current value - history value
32
Delta Check
The absolute delta check is then compared to the absolute delta check set by the
operator (refer to the Settings > Rules > Delta Check Tab > To Change the Absolute
Variation chapter for more information).
If D > absolute value defined by the operator and D > 0, Dabs+ is generated.
If D > absolute value defined by the operator and D < 0, Dabs- is generated.
9.6.3.
You can disable the Delta Check function if you have no use for it. Refer to
the Settings > Rules > Delta Check Tab > To Enable or Disable the Delta
Check Flag chapter of the user manual.
You can change the history validity period of the delta check if you want
to. Refer to the Settings > Rules > Delta Check Tab > To Change the
History Validity Period chapter of the user manual.
Relative Delta Check
The relative delta check refers to the relative difference between the current analysis and
the previous analyses.
D% =
current value - history value
X 100
current value + history value / 2
The relative delta check is then compared to the relative delta check set by the operator
(refer to the Settings > Rules > Delta Check Tab > To Change the Relative Variation
chapter for more information).
If D% > relative value defined by the operator and D% > 0, D%+ is generated.
If D% > relative value defined by the operator and D% < 0, D%- is generated.
9.6.4.
Delta Check Status
Two Delta Check alarms can appear in your results:

the Delta Check + alarm,
the Delta Check - alarm.
The Delta Check + alarm appears when Dabs+ and D%+ have been triggered.
The Delta Check - alarm appears when Dabs- and D%- have been triggered.
Daily Guide
Ref: RAB290CEN
33
Archives
To View Archived Results
10. Archives
10.2. To View Exported Results
10.1. To View Archived Results
Access: Main Menu > Archive
1. To view archived results by date, you need to:

a. Select the date you want to see the results of.
b. Click the results you want to view to open the Report Details screen.
c. Press Return to go back to the result list.
You need to export results either on an FTP site or to an external media before you can
view exported results.
To view exported results, you need to:
1. Press Review Exported Reports.
2. To view archived results by patient, you need to:

a. Press Reports by Patient.
b. Select the patient ID you want.

You can also press Search Patient to look for a specific patient. Press Validate
after you find the patient.
c. Use the up and down scrollbar to see all parameters.
Rejected results display crossed out parameters.
d. Use the left and right scrollbar to see all runs for the patient.
e. Click the results you want to view to open the Report Details screen.
f. Press Return to go back to the result list.
2. Select:
Floppy Disk to view results from a floppy disk (if your instrument is equipped
with a floppy drive)
USB Disk to view results from a USB key
NetWork to view results from the Host
3. Insert your floppy disk or your USB key if you chose one of the first two options.
4. Press OK.
10.3. To Print Archived Results

1. Select the results you want to print.

Daily Guide
Ref: RAB290CEN
34
Archives
To Send Archived Results to the LIS
3. For results by date, select one of the following options:

Print selected reports from list
Print compact daily report list for selected rows
Print all reports from list
Print compact daily report list for all rows

Selected
All
4. Press OK.
4. For results by patient, select Print the patient reports?

5. Press OK.
10.4. To Send Archived Results to the LIS

1. Select the results you want to send to the LIS.

3. Select the Send tab.
Send Selected Results
Send All Results
5. Press OK.
10.5. To Delete Archived Results

1. Select the results you want to delete.

2. Press Delete in the contextual toolbar.
Daily Guide
Ref: RAB290CEN
35
End of Day
Statistics
11. End of Day

Statistics do not include RET analyses.
11.1. Statistics
Access: Main Menu > Status
11.2. To Change Operator

Access: Main Menu
You can log in as another operator without having to restart the instrument. To do so,
you need to:
1. Double-click the second box of the footer.
Beside the status of the reagents and the status of the racks, the Status menu also
provides statistics for the current day, as well as overall statistics for the instrument.
These statistics cover different aspects:
Percentage of run results within normal ranges

Percentage of run results out of the normal range
Percentage of run results out of the panic range
Percentage of CBC runs
Percentage of DIFF runs
Percentage of reruns
Number of Startup cycles
Number of Shutdown cycles
Number of Autoclean cycles
Daily Guide
Ref: RAB290CEN
You are automatically logged-out. To log back in with your current user
name, you need to reenter your password.
2. Log in with a new user account as described in Workflow > Start of Day > Starting
the Instrument > To Log In to the Application chapter.
36
End of Day
Stopping the Instrument
11.3. Stopping the Instrument
3. Specify the shutdown time.
11.3.1. To Perform a Manual Shutdown
11.3.3. To Switch the Instrument Off
Access: Main Menu > ShutDown
1. Press ShutDown.
It is highly recommended to perform a shutdown cycle before switching off the

instrument.
1. Press Quit in the contextual toolbar.
2. Wait during the shutdown cycle.
2. Wait while the instrument shuts down.

When the instrument is ready to be switched off, the following message appears on
your screen: "It's now safe to turn off your computer."
3. Switch off the instrument.
If the system is not used for a period superior to 36 hours, it is mandatory to

power it down. This eliminates startup problems, as well as the possibility of
the dilution chambers evaporating.
11.3.4. To Switch the Printer Off

1. Make sure that no printout has been launched.
11.3.2. To Schedule an Automatic Shutdown
2. Switch the printer off.
Access: Main Menu > Settings > System > Cycle Option
In automatic shutdown mode, the shutdown is launched automatically every day at a

predefined hour. Both Instrument and printer must be switched on 24 hours a day.
1. Press Edit in the contextual toolbar.
2. In the ShutDown area, select Automatic.
Daily Guide
Ref: RAB290CEN
37
End of Day
Stopping the Instrument
Daily Guide
Ref: RAB290CEN
38

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Hematology Analyzer

6. Running Blood Specimen................................................................15

6.1. To Run the Specimen in Rack Mode............................................................ 15

2.1. Contextual Toolbar Description..................................................................... 2

4. Running Quality Control Blood..................................................... 11

11. End of Day...........................................................................................36

Document Date Issued

If a problem occurs or an alarm is displayed during the instrument operation,

Documentation DVD-ROM instructions

2.1. Contextual Toolbar Description

Print/Send: allows to print data.

The main menu includes the following items:

Detail: displays more details regarding the current screen.

Insert: inserts new data.

Delete: deletes an item or data.

2.2. Function Toolbar Description

STOP: stops the instrument (emergency stop).

Alarm: displays the list of alarms.

Worklist: opens the worklist.

Start Rack: runs an automatic sampling.

STAT Mode: runs a manual sampling.

Report: opens the list of reports.

Archive: opens the archived results.

2.3. Main Menu Buttons

Service: opens the service menu.

StartUp: launches a Startup cycle.

Logs: opens the instrument logs menu.

2.4. Logs Menu Buttons

Blank Cycle: displays blank cycle results.

2.5. Quality Assurance Menu Buttons

XB: displays the XB graphs.

Within Run: allows you to perform a repeatability test on the instrument.

Calibration: allows you to calibrate the instrument.

2.6. Service Menu Buttons

Only certified HORIBA Medical technicians have access to the Technician

Miniclean: launches a short rinsing cycle of the counting chambers.

Concentrated Cleaning: starts a concentrated cleaning procedure using

2.7. Settings Menu Buttons

AutoClean: launches a cleaning cycle using ABX Cleaner.

Super User Menu: gives access to customer maintenance.

Technician Menu: (reserved to technicians) gives access to advanced

Save/Restore: allows to save and restore the software configuration.

Users: allows to define operator profiles.

Type Parameter Setting: allows to configure the instrument according to

Soft Parameters: allows to edit software options.

Quality Assurance: allows to define CV for calibration, QC and

2.8. Cycle Selection Button

3.1. To Check the Waste Container Level

Press the ON/OFF switch.

1. Check the level of waste in the container.

Waste must be handled according to your local and/or national regulations.

3.3. Starting the Instrument

To Switch the Instrument On

1. Switch the instrument on.

To Log In to the Application

3.2. To Switch the Printer On

4. Select the following options:

3.3.4.1. To Perform a Manual Startup

2. Select which cycle you want to perform:

To Control the Reagents

Background count limits

Background count limits

If the system is not used for a period superior to 36 hours, it is mandatory to