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Sludge Modelling
Ral Muoz
Pedro Garca Encina
Microbial growth
Exponential Phase:
dX/dt = X
Where:
X = cell concentration (mgDW or
VSS /L)
dX/dt = volumetric cell production
rate (mg/Ld)
= specific growth rate (h-1 or d-1)
Microbial Growth
Xf
rg = dX/dt = X
rg = volumetric cell production
Pf
Sf
time
rg = X = [mS/(Ks + S)]X
max
l-1)
max/2
Substrate concentration
The cell growth rate (and therefore the substrate removal rate) increases
with the substrate concentration, up to a certain level when it stabilize at
m. If the limiting substrate concentration is low: conditions of slow growth!
Endogenous decay
If part of the biomass produced is degraded, rg = - Yrsu is no longer true!
With endogenous decay: rg = -Yrsu kdX
With kd = endogenous coefficient decay (d-1)
rg = Volumetric biomass production rate (g VSS/m3d)
-Yrsu = Rate of biomass production from substrate consumption
kdX = Rate of biomass consumption by endogenous respiration
And = mS/(Ks + S) - kd
Note that rsu is unchanged!!!!!
rsu = -rg/Y kdX/Y = -Y-1(X + kdX) = - maxSX/(Y(Ks + S))
6
at
too
low
substrate
used
for
maintenance
(or
not
detected)
Substrate concentration
Growth rate
Q
X0
S0
V
Vr
Xrr
X
Srr
S
Q
X
S
=D
X = Y(S0 S)
(This could have be obtained directly from the expression of the true
yield)
Exercise
The growth of a strain of Lactococcus lactis on a medium containing
glucose as the growth limiting substrate is characterized by the
following parameters: m = 0.6 h-1 ; Ks = 0.03 g L-1 ; Y = 0.3 g g-1
The feed contains 1g L-1 of glucose. The culture is growing in a 5 L
CSTR being fed at 2.75 L h-1. Using the Monod model, calculate the
steady state values of the dilution rate, the hydraulic residence time,
the glucose concentration (S) and the biomass concentration (X) in the
reactor.
Calculate X and S when:
a. The dilution rate is changed to 0.3 h-1 and 0.6 h-1
b. The substrate concentration is increased to 3 g L-1
10
Solution
By definition D = Q/V = 0.55 h-1 and HRT = 1/D = 1.82 h
Using S = DKs/(m D), S = 0.33 g L-1 and X = Y(S0-S) = 0.201 g L-1
At D = 0.3 h-1 and S0 = 1 g L-1: S = 0.03 g L-1 and X = 0.291 g L-1
At D = 0.6 h-1: S = 1 and X = 0: washout!
11
Cell concentration
Output of cells Dx
Substrate
concentration
S1
S2
max
Dilution rate
Substrate
concentration
max
Dilution rate
CSTR: = D = Q/V
At washout condition: S = S0 = DmaxKs/(m Dmax)
Dmax = mS0/(Ks + S0) = m/(1 + Ks/ S0)
Cell wash-out occurs at too high dilution rates (D >Dmax) and is especially
sensitive at low initial substrate concentration.
12
13
Influence of nbVSS
An amount of non biodegradable, also called inert, VSS is introduced into
the reactor in the WW. This amount is not degraded biologically and
therefore, at a steady state, the nbVSS concentrations in the effluent
and reactor are similar to the nbVSS concentration in the influent (X0,i)
The total mass of VSS in the Bioreactor includes the biomass produced
(rg), the nbVSS introduced (X0,i) and the debris released from the
endogenous decay:
Q
X0
S0
Vr
Xr
Sr
Q
X
S
14
Influence of nbVSS
rVSS = total VSS production rate = rg + QXo,i/V + fd(kd)X
QXo,i/V = Amount of nbVSS in the influent (Q = influent flow rate, X0,i= influent
nbVSS and V = reactor volume).
15
Influence of nbVSS
Fraction of active biomass: Fx,act = X/VSS = rg/rVSS
Indicate how much of the VSS is active (serve to COD removal)
Net biomass yield: Y = - rg/rsu
Observed yield: Yobs = - rVSS / rsu
= COD debris
16
17
Biomass retention
Cell washout is more likely to occur:
At low temperature
At low substrate concentration
At too high flow
What can be done to prevent it?
Large reactors: $$$
Harvest and recycling: activated sludge
Immobilization: biofilm
Biomass retention is almost always needed during WWT:
Large flow rates continuous processes
Low COD concentrations continuous processes risk of
washout!
This is not the case for small WWT!
18
Activated sludge
19
Aeration
Diffused
Surface
$$$ Aeration consumes up to
60% of electricity in a WWTP!
20
Secondary clarifier
Use of classical clarifier
Very important part of the process: the
sludge must have the best settleability
possible, measured by the Sludge
Velocity Index
mg
( Settled Volume of Sludge in 30 min, ml ) (1000
)
L
mL
g
SVI
g
( X , mg )
L
21
22
Clarifier
Effluent
Qe, Xe, Se
Recirculation
Waste
Qr, Xr, Sr
Qw, Xw, Sw
23
Overview
Application
Costs
Advantages
Disadvantages
Organic loading
0.3 - 3 kg BOD/(m3-d)
HRT
4 - 8 h (municipal)
BOD removal
85 - 95% (municipal)
24
Overview
Dissolved O2
Qw
4000-12000 mg/L
Qr/Q0
0.5-0.75
Sludge blanket
Height (/1)
0.1
Typical Operation
Problem
25
SRT =
VX
VX
(QeXe + QwXw)
out
in
Note Xr = Xw
Often Xw >> Xe and QwXw + QeXe QwXw
Other name of SRT: Sludge Age
27
Expression of S
Biomass balance:
VdX/dt = 0 = Q0X0 - (QeXe + QwXw) + Vrg
(QeXe + QwXw) - Q0X0 = VX
From the definition of SRT:
(QeXe + QwXw) - Q0X0 = (QeXe + QwXw)= VX/SRT
1/SRT =
= mS/(Ks + S) kd
S = Ks(1 + (kd)SRT)/(SRT(m kd) 1)
28
Expression of X
X can be obtained from S mass balance:
VdS/dt = Q0S0 (QeSe + QwSw) + rsuV = 0
With the assumptions that S = Sw = Se = Sr (S = bsCOD) and since (Qe
+ Qw) = Q0, this becomes:
Q0(S0 S) = -rsuV
Since rg = -Yrsu kdX; rg = X = X/SRT and HRT = V/Q
X = (SRT/HRT) Y(S0 S)/(1 + (kd)SRT)
29
Qr, Xr,i
Qw, Xw,i
30
32
Nitrifying Microorganisms
2.
3.
4.
5.
Determine the specific growth rate for nitrifyiers, that due to their low
growth control AS design, based on DO and effluent [N-NH4+]
nm N DO
k dn
n
K n N K o DO
SRT 1
6.
7.
PXBio
9.
1 k d SRT
1 k d SRT
1 k n d SRT
10. Calculate the TOTAL VSS and TSS mass in the aeration basin
PMLVSS = rgHV (Heterotrophic X) + rgNV (Nitrifying bacteria) + rdV (debris) +
QX0,I (nbVSS)
PMLSS =[ rgHV (Heterotrophic X) + rgNV (Nitrifying bacteria) + rdV (debris) +
QX0,I (nbVSS)]/0.85 + Q (TSSo-VSSo)
11.
35
Xe 0
Qr
Qr X r (Q Qr ) X
R
Qr
Q
RAS R
X
Xr X
Xr
36
38
Proven efficiency!!
40
Wastewater introduced
in 3 to 4 points
41
Two-sludge process: A first step for BOD removal and nitrification and a
second one for denitrification (supplemented with an external e- donor)
44
Step-Feed
45
[bCOD]
[MLSS]
[rbCOD]
Temperature
46
Q So
F / Mb
Vanox X b
Note: Active biomass Xb is calculated as described in the BOD removal and nitrification section
47
SDNR is also affected by the internal recirculation (IR, typically 3-4) if F/Mb is > 1
Mb
) 0.0078
Mb
) 0.012
48
2.
Determine the SRT from the procedure established for nitrification design
N DO
1
k dn
n nm
SRT
K n N K o DO
3.
Q SRT Y S o S
Xb
V 1 k d SRT
4. Determine IR using the NOx concentration previously determined from the
N balance in the nitrification design and the desired N-NO3- in the effluent
49
Q NO 3 N e Q IR Q R Q
Calculate the amount (kg/d) of nitrate fed to the anoxic tank. The design
in based on the assumption that all the nitrate fed is reduced.
6.
7.
8.
Estimate SDNR based on the F/Mb and rbCOD/bCOD ratios (Fig 8-23 in
Metcalf & Eddy, 2003). Correct for T and IR
9.
Using the SDNRadj and the VAnox selected in step 6 calculate the potential
NO3- removed. Compared with the amount calculated in step 5
10. Repeat the procedure from step 6 by selecting a higher or lower VAnox.
11. Check alkalinity Balance:
[Alkalinity to maintain pH at 7 (typically 80 mgCaCO3)] = [ Influent Alk] + [Alk
produced (typically 3.57 g CaCO3/g N-NO3- reduced)] [Effluent Alk] [Alk
consumed (typically 7.14 g CaCO3/g N-NH4+ oxidized)]
50
1.42
SDNRb
k d 0.5k d ; where k d is the biomass endogeneous
2.86
decay coefficient and the fraction of denitrifying biomass
SDNRb is a function of SRT
SDNRb 0.12SRT
0 .706
SDNRb SDNRb
20 C
1.08(T 20 )
51
52
53
SRT 10-20 d; HRTanox 1-3 h 1st stage 2-4 h 3rd stage HRTaer
(4-12 h 2nd stage and 0.5-1 h 4th stage)
56
P released
during uptake
58
VFA
2- Reduce SRT
3- Add alum or Fe salts in primary treatment (Fe salts remove malodours)
4- Reduce the amount of Nitrate or O2 entering the anaerobic zone
59
61
63
64
ASM n1
Characteristics
Carbon oxidation
Nitrogen removal
Nitrification / Denitrification
Rate of decay b X
Physiological aspects described by decay: cell lysis and
endogenous respiration and predation
Environmental factors (organic substrate, O2, etc..) that affect growth:
Required substances: Modelled as switching functions that
activate or deactivate reactions if the substance is present or
absent:
KO
SO
K O SO
K O SO
ASM Structure
Definitions
The model is presented in a matrix format (Petersen matrix)
Component
Xb
Ss
So
Process rate
Process
1 Growth
2 Decay
-1
-1/Y
(1-Y)/Y
-1
SsXb/(Ks+Ss)
bXb
Model presentation
MASS BALANCES:
Basic equation for mass balances (in terms of COD)
Model Presentation
Continuity check
69
Components in ASM1
Organic matter characterization:
Units (COD)
Division of different substrates based on biodegradability
Soluble/particulate (assumptions)
total COD
COD
biodegradable
SS
XS
COD non
biodegradable
XO
SI
XI
70
Components in ASM1
Nitrogen Characterization (components)
N total
Kjeldahl (TKN)
Ammonia Nl
SNH
Organic N
Organic N
soluble
Non biodegradable
SNI
Nitrates
SNO
Organic N
particulate
Biodegradable
SNS
Non biodegradable
XNI
Biodegradable
XNS
71
Components in ASM1
72
Components in ASM1
The i = 13 column represents Alkalinity
It provides an indication of the buffer capacity of the
wastewater
The balance must include all the reactions that
involve addition or removal of species with proton
accepting capacity
Nitrification tend to decrease and denitrification to
increase
Risk of pH instability at alkalinities < 50 g CaCO3/m-3
Lime addition is often use to maintain the pH
73
Processes in ASM1
Biological processes
j
Process
Heterotrophic decay
Autotrophic decay
Ammonification
Biomass Growth
Biomass decay
Ammonification of organic N
Organic Matter Hydrolisis
74
Processes in ASM1
ORGANIC
NITROGEN
SOLUBLE
ORGANIC
NITROGEN
PARTICULATE
SOLUBLE
INERT
SUBSTRATE
SUSPENDED
INERT
SUBSTRATE
SNS
XNS
SI
XI
NITRATES
AMMONIA
NITROGEN
SNO
SNH
AUTOTROPHIC
BIOMASS
SNI
XBA
READILY
BIODEGRADABLE
SUBSTRATE
HETEROTROPHIC
BIOMASS
SS
XBH
SLOWLY
BIODEGRADABLE
SUBSTRATE
SOLUBLE INERT
NITROGEN
DEBRIS
FROM
BIOMASS
No interaction
XO
Nitrogen Removal
XS
75
ORGANIC
PARTICULATE
NITROGEN
SNS
XNS
INERT
SUBSTRATE
SOLUBLE SI
XI
NITRATES
AMMONIA
NITROGEN
SNO
INERT
SUBSTRATE
SUSPENDED
AUTOTROPHIC
BIOMASS
SNH
INERT SOLUBLE
NITROGEN
XNI
XBA
READILY
BIODEGRADABLE
SUBSTRATE
SS
DEBRIS
FROM
BIOMASS
HETEROTROPHIC
BIOMASS
XBH
XO
7
SLOWLY
BIODEGRADABLE
SUBSTRATE
XS
76
S s (1 YH ) S o i XB S NH YH X BH (1 YH )CO2
SO
1 YH
YH
SS
1
YH
S NH
BH
SS
SO
1 H
K S S S K OH S O
S A LK
i XB
i XB
14
BH
Switching function
Assumptions:
(deactivation at low DO)
Type of substrate (SS). No non-growth associated SS storage
Relationship between catabolic and anabolic reactions (Yield)
N consumption for growth (and associated alkalinity)
77
1 H
2
H H
SS
K S SS
KS
SS
78
SS
XS
X
7 k h BH
X
K X S
X BH
SO
K OH S O
K OH
h
K OH S O
S NO
K NO S NO
X BH
Assumptions:
Extracellular (enzyme dependent: Xs/XBH).
Takes place both in aerobic and anoxic conditions (but at different rates)
79
KH
KH
KH
KX
X S / X BH
K X X S / X BH
X S / X BH
XO
XS
X NS
fP
1 fP
i XB f P i XB
4 bH X BH
Assumptions:
Nitrification
Nitrification Kinetics
ORGANIC SOLUBLE
NITROGEN
SNS
PARTICULATE
ORGANIC
INERT
SOLUBLE
SUBSTRATE
NITROGEN
3
AMMONIA
NITROGEN
SNH
XI
SI
XNS
INERT SUSPENDED
SUBSTRATE
NITRATES
SNO
READILY
BIODEGRADABLE
SUBSTRATE
HETEROTROPHIC
BIOMASS
SS
XBH
INERT SOLUBLE
NITROGEN
AUTOTROPHIC
BIOMASS
sNI
XBA
PARTICULATE
ORGANIC
INERT
XO
SLOWLY
BIODEGRADABLE
SUBSTRATE
XS
82
Nitrification
Aerobic growth of autotrophic biomass (3)
X BA
1
S NH
i XB
S NO
1
YA
S NH
3 A
K NH S NH
1
YA
SO
K OA S O
SO
X BA
S ALK
4.57 Y A
YA
i XB
1
14
7 YA
The oxygen
equivalence for NH4+
Characteristics:
Not depending on organic matter (autotrophs)
Single step nitrification
Alkalinity and oxygen consumption
83
Nitrification
Autotrophic biomass decay (5)
Modeled in the same way as Heterotrophic Biomass decay
X BA
XP
XS
X ND
fP
1 fP
i XB f P i XB
5 b A X BA
84
Nitrification
Organic Particulate Nitrogen Hydrolysis (8)
X NS
S NS
XS
X
BH
kh
X
K X S
X BH
SO
K OH
h
K OH S O
K
S
OH
O
S NO
K NO S NO
X NS
X BH
XS
85
Nitrification
Soluble organic N ammonification (6)
S NH
S NS
S ALK
-1
1 14
6 k a S NS X BH
Characteristics:
First order kinetics
Uncoupled to any biodegradation process.
Alkalinity release (charge balance)
86
Denitrification
Denitrification kinetics
ORGANIC SOLUBLE
NITROGEN SNS
ORGANIC
PARTICULATE
SOLUBLE INERT
SUBSTRATE
NITROGEN
SI
SUSPENDED INERT
SUBSTRATE
XI
XNS
NITRATES
AMMONIA
NITROGEN
SNO
SNH
SUSPENDED INERT
NITROGEN
AUTOTROPHIC
BIOMASS
XNI
XBA
READILY
BIODEGRADABLE
SUBSTRATE
HETEROTROPHIC
BIOMASS
SS
XBH
PARTICULATE
ORGANIC
INERT
XO
SLOWLY
BIODEGRADABLE
SUBSTRATE
XS
87
Denitrification
Heterotrophic Biomass Anoxic Growth (2)
SS
X BH
1
SS
2 H
KS SS
1
YH
S NO
1 YH
2.86 YH
S NH
S ALK
i XB
1 YH
i
XB
14 2.86 YH
14
K OH
S NO
g X BH
K OH S O K NO S NO
Characteristics:
Nitrates as electron aceptor
Alkalinity release for charge balance
Assumption: not all biomass growth in anoxic conditions.
88
89
Stoichiometric Matrix
90
Conclusions
ASM1 is a model for organic matter and
nitrogen removal in activated sludge
biological reactors
Some constraints:
Not valid for P removal
Experimentally unable to estimate:
Conclusions
92
In BioWin3
Biowin 3 allows to simulate AS processes in CSTR.
Bioreactor0
MediaBioreactor
93
In BioWin3
Bioreactor Brush aerator
Similar to a regular bioreactor but aeration
is provided via superficial brush aerator.
Power supply rate is used instead of air
supply rate.
94
In BioWin3
Sequencing Batch Reactor
Cycle setting must be introduced