Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
of Tissues
27 January 2016
Abby R. Whittington, PhD
awhit@mse.vt.edu
Objectives
Cells
Tissues
Organs
What is a Tissue
Organizational intermediate between cells and
organs
An organ may be composed of multiple types of
tissues
Tissues are composed of: Extracellular Matrix
Cells
Extracellular Matrix
The Body as a
achi e
Types of Tissues
1. Epithelial Tissue
- Densely packed cells, little intercellular space
- Protect tissues and regulate the exchange of
chemicals
2. Connective Tissue
- Fibrous tissues, gives shapes to organs,
connection of body tissues
3. Muscle Tissue
- Active contractile tissue of the body
4. Nervous Tissue
- Sensory input, integration, control of muscles
and glands
Epithelial Tissue
Blood Vessels
Esophagus
Kidney Tubules
Sweat Glands
Digestive Tract
Gland Ducts
Simple
- Single Layer
- Secretion, Absorption
Stratified
- Multiple Layers
- Protection
Respiratory
Epithelial Examples
Human Skin
-Stratified Squamous Epithelial
Small Intestine
- Simple Columnar Epithelium
Connective Tissue
Loose Fibrous
Areolar
- Binds epithelia to other tissues
- very abundant
- lose, elastic
Adipose
- Stores energy
- protection
Reticular
- Support lymphoid organs
- Porous, support free blood cells
Dense Fibrous
Supportive
Cartilage
- Deformable in compression
- Shock absorber
- High water content
- hydrogel like ature
Bone
- Rigid
- Provides support and protection
- Highly mineralized
- Structurally similar to reinforced
concrete
- Contains bone marrow
Fluid - Blood
Muscle Tissue
Smooth Muscle
Skeletal Muscle
Contractile tissues
- Skeletal muscle is voluntary
- Smooth and cardiac muscle is involuntary
- Striations present in skeletal and cardiac muscle -> short intense bursts
Cardiac Muscle
Nervous Tissue
- Motor Neurons
- Axon and Dendrite
- Long, up to a meter
- Neuroglia
Histology
Sample Preparation
Fixation
Formalin
Paraformaldehyde
Embedding
Paraffin Wax
Glycol
Sectioning
Microtome or Cryostat
Section Orientation
Staining
Hematoxylin & Eosin (H&E)
Masso s trichrome
Giesmsa
Nuclei -> violet, cytoplasm -> pale blue, erythrocytes -> pink
M
B
B=
BM =
M=
BM
Red =
Green =
______ of bone
___________________________ of
articular cartilage
__________________________
of myelinated nerve bundle
___________________of
Bladder muscle
C
C=
M=
Black =
Brown =
_______ = pink
_______ = purple
Immunohistology
Summary
Cells and extracellular matrix compose tissues
Four main tissue types exist in the body
Epithelium
Connective
Muscular
Nervous