COSMOSIL

COSMOSIL
COSMOGEL
COSMOGEL
Reversed Phase High Performance Liquid Chromatography

COSMOSIL PNAP Packed Column

COSMOSIL PNAP is a Reversed Phase HPLC Column with Naphthalene bounded silica packing material. Naphthalethyl group has two aromatic rings and has strong P-P interaction. This column offers improved separation of
compounds such as positional isomers that are difficult to separate with Alkyl group bonded materials.
Features of COSMOSIL PNAP

- Naphthalene bonded silica packing material

- Stronger P-P interactions than Phenyl column

Material characteristics
Packing material
Silica gel

COSMOSIL PNAP
high purity spherical porous silica

Average particle size

Average pore size

5Mm
approx. 120

Specific surface area

Stationary phase

approx. 300m2/g
naphtylethyl group

Bonded type
Main interaction

monomeric type
P-P interaction

End-capping treatment
Carbon content

treated

Naphtylethyl group
Si

CH3

H3C

approx. 11%

Comparison with Phenyl columns

COSMOSIL PNAP with two aromatic rings has storonger P-P interactions than Phenyl column which has one aromatic
ring.

Ph
Si
H3C

PNAP

PE-MS
Si

CH3

H3C

Si

CH3

H3C

CH3

The difference of interaction between

benzene and nitrobenzene

PP

Stationary phase
Strong P-P interaction
PNAP

WeakP-P interaction
Ph, PE-MS

H
H

H
C

NAP

C
C

C
C
H

Strong

NO2

1.00

Benzene

1.20

1.40

(Nitrobenzene/Benzene)

1.60

Nitrobenzene

C
H

C
H

Sample

Ph

C
C

C
H

Weak

PE-MS

H
C

Application data

Position isomers of Tolunitrile

CH3

5C18-MS

5PE-MS

 NAP

(40%Methanol)

(40%Methanol)

(50%Methanol)

CH3

CH3

CN

CN
CN

m-

o-

po-

p-

po-

m-

Column size
Flow rate
Temperature
Detection
Sample

m-

o-

m-

p-

4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
o-Tolunitrile (2.0 g)
m-Tolunitrile (2.0 g)
p-Tolunitrile
(2.0 g)

Position isomers of Phthalonitrile

5C18-MS

5PE-MS

(30%Methanol)

(40%Methanol)

 NAP

CN

CN

CN

CN

(50%Methanol)

CN
CN

o-isomer

o-, p-

m-isomer

p-isomer

ppm-

o- mo- m-

Column size
Flow rate
Temperature
Detection
Sample

4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
Phthalonitrile
(0.3 g)
Isophthalonitrile (3.0 g)
Terephthalonitrile (0.15
g)

Tocopherol
R1

5C18-MS

5PE-MS

 NAP

(95%Methanol)

(90%Methanol)

(90%Methanol)

HO
R2

O
R3

R1

Me

Me

R2

Me

Me

R3

Me

Me

Me

Me

Column size
Flow rate
Temperature
Detection
Sample

4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 295nm
Tocopheril ( )
(1.25 g each)

Adrenal cortex hormone

5C18-MS

5PE-MS

PNAP

(50%Methanol)

(50%Methanol)

(60%Methanol)

OH

HO

1.Prednisolone

OH

2.Hydrocortisone

Column size
Flow rate
Temperature
Detection
Sample

HO

OH
O

HO

O
HO
HO

3.Cortisone

4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 254nm
1. Prednisolone (0.33Mg)
2. Hydrocortisone (0.33Mg)
3. Cortisone (0.33Mg)

Isoflavone
5C18-MS-II

5PE-MS

PNAP

Column size
Mobile phase

4.6mmI.D. x 150mm
A:Acetonitrile/20mmol/l
Phosphate Buffer(pH2.5)= 10/90
B:Acetonitrile/20mmol/l
Phosphate Buffer(pH2.5)= 50/50
B0100%/20min linear gradient
1.0ml/min
30oC
UV 260nm
1. Daidzin (0.05Mg)
2. Glycitin (0.05Mg)
3. Genistin (0.05Mg)
4. Daidzein (0.05Mg)
5. Glycitein (0.075Mg)

6. Genistein (0.025Mg)

Flow rate
Temperature
Detection
Sample

Berberine
5C18-MS

5PE-MS

OMe

OMe

N+

MeO
OMe

Cl-

1.Palmatine Chloride

N+

MeO
OMe

Cl-

2.Berberine Hydrochloride

Column size 4.6mmI.D. x 150mm

Mobile phase 5C18-MS-II : Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 40/60
5PE-MS
: Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 60/40
NAP : Methanol/20mmol/l
Phosphate Buffer(pH2.5)= 80/20
Flow rate
1.0ml/min
Temperature 30oC
Detection
UV 254nm
Sample
1. Palmatine Chloride (0.2Mg)
2. Berberine Hydrochloride (0.2Mg)

Futher separation
COSMOSIL PYE is a packed column with pyrene bonded silica packing material. It has stronger P-P interaction than
PNAP since pyrene has four atromatic rings.

Isoflavone
R

5C18-MS

PNAP

(98%Methanol)

(90%Methanol)

(95%Methanol)
HO

R=

1.Cholesterol

2.Stigmasterol
Si

3.Campesterol
4.Sitosterol

Column size
Flow rate
Temperature
Detection
Sample

4.6mmI.D. x 150mm
1.0ml/min
30oC
UV 210nm
1. Cholesterol
2. Stigmasterol
3. Campesterol
4. Sitosterol

Ordering information
Product name
COSMOSIL PNAP
Packed Column

Size
1.0mmI.D. x 150mm
1.0mmI.D. x 250mm
2.0mmI.D. x 150mm
2.0mmI.D. x 250mm
3.0mmI.D. x 150mm
3.0mmI.D. x 250mm
4.6mmI.D. x 50mm
4.6mmI.D. x 100mm
4.6mmI.D. x 150mm
4.6mmI.D. x 250mm
10.0mmI.D. x 150mm
10.0mmI.D. x 250mm
20.0mmI.D. x 150mm
20.0mmI.D. x 250mm
28.0mmI.D. x 250mm

Code No.
08076-61
08077-51
08078-41
08079-31
08080-91
08081-81
08083-61
08084-51
08085-41
08086-31
08088-11
08089-01
08092-41
08093-31
08095-11

Product name
COSMOSIL PNAP
Guard Column

Size
4.6mmI.D. x 10mm
10.0mmI.D. x 20mm
20.0mmI.D. x 20mm
20.0mmI.D. x 50mm
28.0mmI.D. x 50mm

Code No.
08082-71
08087-21
08090-61
08091-51
08094-21

Other size may be available. Please enquire!!

For research use only, not intended for diagnistic or drug use.

NACALAI TESQUE, INC.

Hichrom Limited

Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855, JAPAN

The Markham Centre, Station Road
TEL:1+81-75-251-1730
Reading, Berks, RG7 4PE
FAX:Theale,
+81-75-251-1763
Tel: 0118 930 3660
Web site: http://www.nacalai.com
Fax: 0118 932 3484
E-mail:
info.intl@nacalai.co.jp
Web
site: www.hichrom.co.uk
Email: sales@hichrom.co.uk