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International Journal of
Pharmaceutical Research and
Life Sciences
IJPRLS
INTRODUCTION
In recent years, evidence of cases of insulin
resistance and the occurrence of side effects from
prolonged administration of conventional drugs have
triggered the search for safe and effective
alternatives. Nowadays, medicinal plants play an
important role in the treatment of diabetes mellitus.
Several plant extracts and isolated phytochemical
have been examined for the antidiabetic activity,
with a view to identify alternative treatment
strategies for diabetes. It has been observed that,
certain resistant cases of diabetes, that do not
respond well to conventional drugs often respond
well
to
supplementation
with
natural
1
remedies .Seeds of Trigonella foenum graceum
(fenugreek) commonly known as methi belongs to
the family Leguminosae. It is used as food and also
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RESULTS
The glucose adsorption capacity of aqueous extracts
of trigonella foenum-graceum seeds& boerhaavia
diffusa are shown in table No.1. A series of different
concentrations of glucose (5-100mM) were used for
determining the adsorption capacity of samples for
glucose. The results have showed that aqueous
extracts of seeds of Trigonella foenum graceum
showed significantly higher glucose adsorption
capacity at 100mM when compared to Boerhaavia
Diffusa. The effect of aqueous extracts of trigonella
foenum-graceum seeds& boerhaavia diffusa on
glucose transport across yeast cell membrane was
studied in an invitro system comprising of yeast cells
suspended in glucose solution of varying
concentration (5-100mM) in presence of extracts.
The percentage increase in glucose uptake by yeast
cells is presented in Table No. 2,3,4,5 & 6.The
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Renu Anna Joseph. et al/ International Journal of Pharmaceutical Research and life Sciences. 1(2), 2013, 174 - 179.
Table No.1: Glucose Bound by Medicinal Plant Extracts in Different Concentrations of Glucose
GLUCOSE BOUND (mM)
SAMPLE
5mM
15mM
25mM
50mM
100mM
3.9210.6
12.2100.7
13.5121.7
38.4152.3
79.9213.5
Boerhaavia diffusa
1.4760.3
13.2450.5
14.9950.9
29.9902.4
60.9802.4
1mg/ml
2mg/ml
3mg/ml
4mg/ml
5mg/ml
80.2280.4
81.4291.3
82.7152.4
83.0101.4
85.7533.2
Boerhaavia diffusa
77.3020.6
78.6891.2
80.9252.7
81.1431.4
84.8542.8
1mg/ml
2mg/ml
3mg/ml
4mg/ml
5mg/ml
79.4480.5
80.6441.2
81.8232.4
82.7383.0
83.1462.5
Boerhaavia diffusa
74.5490.6
77.9121.3
79.0582.8
80.4371.9
82.6632.8
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Table No.4: Showing Percentage Increase in Glucose Uptake by Yeast Cells in 25mm of Glucose
% INCREASE IN GLUCOSE UPTAKE
SAMPLE
1mg/ml
2mg/ml
3mg/ml
4mg/ml
5mg/ml
77.7321.7
77.8422.0
78.7182.6
79.9042.7
79.9995.5
Boerhaavia diffusa
72.9351.2
75.3142.5
77.1122.6
79.8422.3
79.8425.2
1mg/ml
2mg/ml
3mg/ml
4mg/ml
5mg/ml
72.1121.5
76.4202.7
77.1204.1
77.8544.8
78.1125.4
Boerhaavia diffusa
70.0101.5
72.5102.9
75.5054.7
78.0104.9
78.4324.8
72.1121.5
76.4201.9
77.1201.6
77.8543.0
78.1123.7
Boerhaavia diffusa
70.0101.8
72.5101.5
75.5053.7
78.0102.9
78.4324.9
Renu Anna Joseph. et al/ International Journal of Pharmaceutical Research and life Sciences. 1(2), 2013, 174 - 179.
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