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Objectives:
1. To investigate the effect of light intensity on the rate photosynthesis by
measuring oxygen evolved
2. To investigate the effect of carbon dioxide concentration on the rate of
photosynthesis
Precaution
On the day of the experiment, cut 10 cm lengths of Elodea, put a paper-clip
on one end to weigh them down and place in a boiling tube of water in a
boiling tube rack, near a high intensity lamp, such as a halogen lamp or a
fluorescent striplight. Check the Elodea to see if it is bubbling. Sometimes
cutting 23 mm off the end of the Elodea will induce bubbling from the cut
end or change the size of the bubbles being produced
There are various set-ups that can be used to measure the rate of
photosynthesis, each relies on counting the oxygen produced during the
reaction. To improve results, add a pinch of baking soda to the water in the
test tube. Cut elodea stems at an angle and use your fingers to crush the
end of the stem. The water in the beaker is meant to absorb the heat from
the light.
3. 20cm
4. 10cm
Part B: CO2 Concentration vs Light
1. Set up the apparatus as shown in a darkened room.
2.
Look for a stream of bubbles coming from the cut end of the pondweed.
3. Count the number of bubbles produced in 1 minute. Repeat twice and calculate a mean
bubble count number of bubbles per minute. This is the mean rate of bubble production
Colour of light.
Temperature