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Adolfo, Angela Nicole G., Alcaraz Frances Jillian F., Aliman, Stephanie T., Astor, Jashmin D.,
Bauzon, Franchesca D., Cabral, Danica Rose G.
separates
Diverse
types
of
substances
partition
experiment.
have
different
Chromatography
applications.
analytical
be
used for
separations.
separates
preparative
In
every
scale
only
can
column
of
(Load),
of
separate
analytical
relative
type
Not
separat ion
large
quantities
methods
such
of
sample,
as
paper
easier to perform.
is
over
considered
most
other
elute
column.
through a
chromatography
column
mixture
or
column
components
METHODOLOGY
pipettes,
vials,
which
beakers,
is
watch
our
glass,
extract.
DCM
First,
set
up
the
Column
Column chromatography:
2)
following
Rf =
colors
were
then
placed
Orange pigment
32
0.54
3)
Rf =
0.83
CONCLUSION
Table 2 - Thin Layer Chromatography
Color of
Component
1
2
3
4
Yellow
Orange
Pale Yellow
Dark Orange
Distance of
component
from origin
(X) in cm
7 cm
32 cm
NA
4 cm
0.13
0.54
NA
0.83
Computations:
REFERENCES
1)
Rf =
Yellow pigment
7
5
0.13
Website:
Chemistry: Chromatography. (2012,
August). Retrieved from http://www.marz-
kreations.com/Chemistry/Chromatography/
Dyes/RF-Values.html
Books:
Robards, K., Haddad,P.R., Jackson,P.E.,
(1994). Principles and Practice of Modern
Chromatographic Methods. San Diego,CA:
Academic Press Inc.
York:
DOCUMENTATION