05.1256 Accelerate Protein Sample Prep

www.pall.
com/lab
Accelerate Protein Sample

Preparation and Analysis
Products for Proteomics, Biopharmaceutical
Research, Diagnostics, and Protein Chemistry
Inspiring Confidence in
Critical Proteomic Steps
Reliable sample preparation and fractionation processes are
key to the success of proteomic research. Effective steps
can facilitate the discovery of life-saving and life-enhancing
diagnostic and pharmaceutical products. Ineffective steps
can lead to loss of valuable samples and time.
Pall Life Sciences manufactures membranes and
chromatography resins that exhibit high resolution
and binding capacities with low non-specific binding
for the purification and concentration of proteins. We
incorporate these media into devices that use fast, gentle
methods and minimize handling to protect your samples.
Our attention to product quality and performance is
expressed in reproducible, reliable results.
Pall also offers a variety of products for protein detection
including PVDF and nitrocellulose membranes for western
blotting, available as cut membranes or integrated into
multi-well filter plates.
The results of my work can change peoples lives.

Thats why I count on Pall technologies.
Optimized
Pall offers the largest selection of membrane and
chromatography resin chemistries and manufactures
these under precise, highly controlled conditions to
ensure product quality. Applying a unique blend of Lean
Manufacturing and Six-Sigma principles, our quality
assurance procedures result in superior products with
exceptional lot-to-lot reproducibility. Pall is one of the few
companies to control device manufacturing through all
stages, from media production to housing material selection
to final device assembly. This gives us a distinct quality
advantage, allowing us to maximize processing accuracy,
speed, safety and reliability.
When we need support, Pall has the

resources that link service to science.
Global reach
Pall Corporation is the largest filtration, separation and
purification company in the world. Our diversity and global
reach provide us with unique application insights and
Scaleable
product development opportunities that we use to benefit

our customers. Pall scientific laboratories worldwide work to
Whether you are processing a single sample or detecting

thousands of samples, Pall offers a variety of device
configurations to support your techniques. And when
scale-up is a factor, Pall offers product platforms that
incorporate the same membranes and materials of
solve complex customer problems, test products, explore

new product applications, and provide ongoing technical
support. Our global manufacturing facilities and distribution
networks ensure product availability, easy ordering, and
fast delivery.
construction to allow precise scale-up of processing

volumes from lab to process scale.
Personalized solutions
Automation compatible
With over 50 years of experience, Pall knows how to

combine the optimal materials to improve performance in
Palls high throughput products meet industry guidelines

to ensure compatibility with all standard robotic equipment.
Our filter plates meet the ANSI/SBS X-2004 specifications
for smooth operation and worry-free performance. We
partner with industry-leading equipment manufacturers
to develop products and optimize the automation for
both routine and unique applications.
a range of applications. Our goal is to provide you with the

best products for your application and help you use them
to their fullest potential. Although we offer thousands of
standard product configurations, Pall product teams
work diligently with our clients and other industry
leaders to develop leading-edge technologies and
optimize product configurations.
www.pall.com
Addressing the Challenges of

Protein Sample Preparation
Application guide
CHROMATOGRAPHY PRODUCTS (Pages 8 - 13)
Ultrogel AcA, Trisacryl GF Size Exclusion
Chromatography Resins
Sample complexity reduction is an important first step to facilitate access to
Ceramic HyperD Ion Exchange Chromatography Resins
the low abundant proteins of interest for disease research and diagnostics.
Trisacryl, HyperD, HyperCel Affinity Chromatography Resins
The process for human serum and plasma frequently includes depletion of
SDR HyperD Solvent-Detergent Removal Resins
highly abundant proteins such as albumin and IgG in combination with other
MEP HyperCel Hydrophobic Charge Induction

fractionation technologies prior to 2D-Gel or LC-MS/MS separation. This can
Mustang Ion Exchange Membrane Devices
be achieved with a combination of specific affinity ligand-based depletion and
Omega Ultrafiltration Membrane for Size Exclusion
ion exchange chromatography-based fractionation technologies. Pall provides
MULTI-WELL FILTER PLATES (Pages 14 - 17)
separation technologies and devices to address these challenging needs in
AcroPrep Plates with Hydrophilic Filtration Membranes
both low and high throughput applications.
AcroPrep Plates with Ion Exchange Membranes

AcroPrep Plates with Prefilters
AcroPrep Plates with Ultrafiltration Membranes for Size Exclusion
AcroPrep Plates with Glass Fiber
AcroWell Plates with Binding Membranes
CENTRIFUGAL DEVICES/SPIN FILTERS (Pages 18 - 19)
Microfiltration Centrifugal Devices
Ultrafiltration Centrifugal Devices
PROTEIN PURIFICATION KITS (Pages 20 - 21)
Enchant Kit for Albumin Depletion
Enchant Kits for IgG Purification
Enchant Multi-Protein Affinity Separation Kit
TANGENTIAL FLOW FILTRATION PRODUCTS (Pages 22 - 25)
Minimate TFF Capsules
Ultrasette Lab TFF Devices
LV Centramate Lab TFF Systems
Centramate Lab TFF Systems
PREFILTRATION/CLARIFICATION (Pages 26 - 27)
Acrodisc Syringe Filters with Prefilters
VacuCap Vacuum Filtration Devices
DETECTION PRODUCTS (Pages 28 - 29)
Vivid Gene Array Slides
FluoroTrans PVDF Membranes
BioTrace PVDF Membrane
BioTrace NT Nitrocellulose Membrane
UltraBind Affinity Membrane
Immunodyne ABC Membrane
Sample preparation...its the last thing I want to worry

about but the first thing on my mind. Thats why I trust Pall.
ARRAYS (Pages 13, 29)

Protein Chip System Based on Arrays
Sample Preparation
Optimization
Sample Detection
In combination with appropriate chromatography resin.
www.pall.com
Blo
ttin
Im
g
mu
no
as
sa
ys
Co
va
l
e
Bin nt
din Pro
g tein
We
ste
rn
Ab
un
da
nt
Pro
tei
Pro
nR
tei
nF
em
rac
ov
al
t
i
on
Pro
ati
tei
on
nC
on
ce
Pro
ntr
ati
tei
on
nD
es
a
l
t
Bu
ing
ffe
rE
xc
ha
ng
De
e
ter
Im gen
pu t a
rity nd
Re
mo
Fil
va
tra
l
t
i
an on,
d L Cl
ys arif
ate ic
Cle atio
Pro
ari n,
tei
ng
n
Tra P
dit urif
ion ica
al tio
Ch n b
Pu
rom y
rifi
ato
Ta catio
gra
gg n
ph
ed of
y
Pro
tei
ns
Sc
ou
tin
g
Pro
ce
ss
Pro
teo
Sc
mi
ale
cs
-u
p
Finding the Perfect Fit

For Your Application
Pall has thousands of media chemistries from which to
select, and we are constantly modifying and developing
new chemistries to meet your specific application
challenges. Combined with superior housing materials
in a multitude of configurations and industry-leading
research, development, and manufacturing resources,
Pall is uniquely positioned to deliver products exactly
to your specifications.
Versatile separation technologies

Pall gives you more filtration and separation material
options than any other organization in the world. This
means you have the best technologies available to optimize
Reliable device configurations
your application performance. Our quality control procedures
While the heart of Pall is media development and
result in the production of media with exceptional lot-to-lot
manufacturing, the design and manufacture of devices
reproducibility and uniformity to give you the consistent,
is the ultimate expression of Pall technologies. This is
accurate results you require.
where it all comes together high performance media,

outstanding housing materials, and devices designed to
maximize processing accuracy, speed, safety, reliability,
and ease of use.
The charts on pages 5 and 7 will help you evaluate
media and device options based on your applications
and desired material characteristics.
Protein purification
and analysis media
Ca
p
Co ture,
nc Re
e
Hig ntra leas
te e,
h
Ac Nu
id cle
Bin ic
Hig di
h P ng
rot
ein
Lo
Bin
w
din
Pro
g
tei
nB
Bin
ind
ds
ing
Le
Vir
uk
o
a
Ca l Par cytes
ptu tic
re/ le
Re
Hy
dro leas
e
ph
ob
Hy
i
dro c
ph
ilic
Co
m
Ha pati
rsh ble
C w
Ch hem ith
rom
ica
ls
ato
gra
Fil
ph
tra
y
tio
n
Pre
filt
rat
ion
Characteristic
Separation
Technologies
Membrane
Name
Medium
Microfiltration
Bio-Inert Membrane
Modified Nylon
Hydroxyl
Fluorodyne II Membrane
PVDF
Hydroxyl
GH Polypro Membrane
(GHP)
Polypropylene
Proprietary
HT Tuffryn Membrane
Polysulfone
Proprietary
LoProdyne LP Membrane
Nylon
Hydroxyl
Pallflex Media
Glass Fiber
Varies
PTFE Membrane
PTFE
PTFE
Supor Membrane
Polyethersulfone
Proprietary
Supor R Membrane
Polyethersulfone
RepelTM Treated
Versapor Membrane
Acrylic Copolymer Proprietary

on a Nonwoven
Support
Versapor R Membrane
Acrylic Copolymer Repel Treated

on a Nonwoven
Support
Ultrafiltration
OmegaTM Membrane
Modified
Polyethersulfone
Chromatography
BioSepra Size Exclusion

Resins (Ultrogel, Trisacryl)
Varies
Fractionation, purification, desalting
BioSepra Q Ion Exchange

Resins (HyperD)
Composite
Quaternary
Material (ceramic) Amine
Protein concentration, protein fractionation,

contaminant removal, separation based on charge
BioSepra S Ion Exchange

Resins (HyperD)
Composite
Sulfopropyl
Material (ceramic)
BioSepra DEAE Ion

Exchange Resins (HyperD)
Composite
Diethylaminocthyl
Material (ceramic)
BioSepra CM Ion Exchange

Resins (HyperD)
Composite
Carboxymethyl
Material (ceramic)
Surface
Chemistry
Proprietary
Filtration of protein solutions, low protein binding,

general filtration, clarification of cell lysate and
tissue homogenates
Filtration of low concentration proteins,

sample preparation, general filtration
Time-resolved fluorescence, bead-based assays,

fluorescent detection of analytes, sample prep
prior to HPLC, general filtration of aqueous
and organic solvents
Filtration of low concentration proteins,

sample preparation
Prefilters, DNA extraction
Proven performance in documented procedures
Vent filters, chemical and molecular synthesis

General filtration applications, low protein binding,
bead-based assays
Vent filters
General filtration applications
Vent filters
Sample preparation, PCR cleanup, sequencing

cleanup, ultrafiltration separations, nucleic acid and
protein purification, concentration and fractionation
BioSepra Affinity Resins

Varies
(Blue Trisacryl M, Protein A Ceramic
HyperD F, Heparin HyperD M,
Lysine HyperD, IMAC HyperCel)
Varies
Abundant protein removal, lgG purification,

glycoprotein enrichment, lipoprotein purification
BioSepra Solvent-Detergent
Removal Resins (SDR HyperD)
Composite
Material (silica)
Hydrophobic
Polymer Moiety
Detergent removal
BioSepra Hydrophobic Charge

Induction Chromatography
Resins (MEP HyperCel)
Cellulose Polymer 4-Mercaptoethyl-pyridine
Purification of poly and monoclonal antibodies of

various species, enzymes and recombinant proteins
Mustang E Membrane
Polyethersulfone
Quaternary
Ammonium
Removes endotoxin from buffers, water, neutral

sugar solutions, and certain biological solutions
Quaternary
Amine
(positively-charged)
Strong anionic exchanger for DNA clearance,

nucleic acids and negatively-charged proteins,
viral particle purification/concentration
Polyethersulfone
Sulfonic Acid
Strong cationic exchanger for positivelycharged proteins, viral particle

purification/concentration
Mustang Q Membrane
Mustang S Membrane
Polyethersulfone
(negatively-charged)
Binding
Recommended Applications
Biodyne A Membrane
Nylon 6,6
(amphoteric)
Biodyne B Membrane
Nylon 6,6
Quaternary
Macroarrays, microarrays, Southern blots,

northern blots, dot blots, reverse dot blots, DNA
fingerprinting, binds negatively-charged molecules
Reverse dot blots, ELISA,

binds positively-charged molecules

northern blots, dot blots, DNA fingerprinting, ELISA
(positively-charged) Ammonium
Biodyne C Membrane
Nylon 6,6
Carboxyl
(negatively-charged)
Biodyne Plus Membrane
Nylon 6,6
Quaternary
Ammonium
BioTraceTM NT Membrane
Nitrocellulose
BioTrace PVDF Membrane
PVDF
FluoroTrans PVDF Membrane
PVDF
FluoroTrans W Membrane
PVDF
Modified Nylon
Proprietary
Activated
Surface
Leukosorb Membrane
Proprietary
Proprietary
UltraBindTM Membrane
Modified
Polyethersulfone
Aldehyde
Western blots, colony/plaque lifts,

Southern blots, protein/nucleic acid dot blots,
flow-through diagnostic tests, northern blots
Western blots, protein dot blots
N-terminal protein sequencing, lowest levels
of autofluorescence

northern blots, dot blots, reverse dot blots, DNA
fingerprinting, colony and plaque lifts, ELISA
Western blots, Southern blots
Oligonucleotide arrays, reverse dot blots,

protein arrays, immunoassays
Leukodepletion, nucleic acid extraction,

in situ PCR
Affinity chromatography,
ELISA, ELISPOT
(unsupported)
www.pall.com
Chromatography Products
Expand Separation Options
Pall offers chromatography products to facilitate research
portfolio and expands our offering to include resins. The
needs, scale-up, and polishing. Our chromatography
BioSepra line of chromatography resins greatly simplifies
solutions are available in resin or membrane formats to
protein purification and fractionation. These broad lines of
support your specific application. Choose from our extensive
chromatography products exhibit superior performance
portfolio of media including flat sheet membrane, bulk resin,
and are useful for affinity, ion exchange, size exclusion,
and media incorporated into specific product housings. Pall
and hydrophobic interaction chromatography (HIC).
products allow you to tailor your product selection to the
Unique mixed-mode BioSepra products also exist to
nature of the purification you desire.
provide solutions to current sample preparation challenges

such as detergent removal and antibody purification.
BioSepra resins facilitate

high capacity purification
Chromatography continues to be an essential technology
for the purification of biomolecules. Palls recent acquisition
of BioSepra products complements our current technology
Special features
True Scalability
The resins Pall offers for small-scale discovery
applications are the same ones offered to our
customers currently manufacturing biopharmaceuticals. The ability to scale up is essential for
those working in drug discovery, development, and
manufacturing. These resins can be used in varying size
chromatography columns, as well as in batch mode for
single prep or high-throughput mode. This is ideal for
quick preps or in situations where optimizing purification
conditions is required.
Versatile Product Line
Pall bottled resins can be used for small and large sample
sizes involving single use or high throughput methods of
purification. Our base resin varies depending on targeted
applications. These resins can be used in combination
with Pall device configurations such as multi-well filter
plates and spin devices.
High Binding Capacities and Fast Flow Rates
By tailoring attributes such as chemistry, pore size,
and resin diameter to specific applications, Pall
chromatography resins exhibit the highest
performance characteristics possible while
ensuring reliable, reproducible protein isolation.
BioSepra Ion Exchange Resins Exhibit

Extremely High Dynamic Binding Capacity
Media
Q Ceramic HyperD 20
DEAE Ceramic HyperD F
S Ceramic HyperD F
S Ceramic HyperD 20
CM Ceramic HyperD F
Flow Rate (mL/min)

1
5
106.0 mg/mL
91.5 mg/mL
101.5 mg/mL
87.5 mg/mL
80.5 mg/mL
61.5 mg/mL
98.0 mg/mL
89.5 mg/mL
108.0 mg/mL
87.5 mg/mL
10
82.5 mg/mL
77.5 mg/mL
53.5 mg/mL
83.5 mg/mL
73.5 mg/mL
Applications
Detergent removal (SDR HyperD Resin)
Protein fractionation (Q, S, DEAE, CM Ceramic HyperD Resins)
The HyperD line of ion exchange resins shows high dynamic

binding capacity (50-110 mg BSA/mL of resin for resins tested
here). Dynamic binding capacity is measured in a 1 mL packed
column by pumping BSA (anion) or lysozyme (cation) at 5 mg/mL
in a suitable binding buffer until the column capacity is exceeded.
The capacity is then calculated by estimating the volume of protein
required to achieve this breakthrough and expressed as mg/mL
media volume. Anion and cation chemistries are available in both
50 m (HyperD F resins) and 20 m (HyperD 20 resins) particle
sizes for improved resolution.
Efficient Removal of Detergents from Protein

Solutions Using the BioSepra SDR HyperD Resin
IgG purification from various sample types

(Protein A Ceramic HyperD F Resin)
IgG purification from cell culture supernatant
(MEP HyperCel Resin)
Albumin depletion (Blue Trisacryl M Resin)
Desalting (Trisacryl GF05 M and Ultrogel AcA 202 Resins)
Tagged biomolecule purification (IMAC HyperCel Resin)
Reference material
Sell Sheet, BioSepra Chromatography Media, PN 33400
Detergent
Triton (DBC = 60-80 mg/mL)
Initial Conc. (ppm)
Final Conc. (ppm)
Removal Efficiency
Protein Solutions
AT-III
IgG
10,000
10,000
< 10
< 10
> 99.9% > 99.9%
Bovine Serum
10,000
340
> 95.2%
SDR HyperD resin binds detergents used in viral inactivation

processes (e.g., TnBP and Triton* X-100), as well as other
common detergents used in protein procedures (e.g., CHAPS,
SDS, ASB14). High recovery of proteins (exclusion limit 10 kDa)
is obtained. This product exhibits high adsorption capacity
for small hydrophobic molecules and is stable in acidic,
polar organic and oxidizing solutions.
www.pall.com
Membrane devices for ion

exchange chromatography
speed processing
Membranes are recommended in chromatography
applications when there is a need to purify large molecules
or in situations where faster flow is required. Membrane
chromatography is extremely economical because flow rates
are significantly faster than traditional resin chromatography,
decreasing processing time and increasing throughput. Palls
Mustang membranes possess large convective pores and
Special features
Scaleable
For laboratory-scale applications, Mustang membranes are
available in Acrodisc units for single samples and AcroPrep
96-well filter plates for high throughput sample processing.
Devices with Mustang S and Mustang Q membranes can be
scaled up to larger-capacity capsules and cartridges from Pall.
have dynamic binding capacities that are relatively insensitive

to the effects of high flow rates, even for large molecules
Application-specific Membrane Chemistries
such as plasmids and viruses.
Mustang Q membrane is a strong anion exchanger that

effectively binds plasmid DNA, negatively-charged proteins,
and viral particles. Mustang S membrane is a strong cation
exchanger that effectively binds positively-charged proteins
and viral particles.
Fast Flow Rates for Rapid Separations
Mustang membranes withstand high flow rates to render
faster purification without affecting recovery rates.
10
Acrodisc Unit with Mustang Q Membrane:

Resolution with BSA and Goat lgG
25
Absorbance
Conductivity
25
Goat IgG
20
BSA
20
15
Unbound Protein
from Goat IgG
15
10
10
5
5
0
min
Conductivity (ms/cm)
Absorbance 280 nm (mAU)
30
0
0.6
1.2
1.9
2.5 3.1 3.8

Time (min)
4.4
5.0
5.7
6.3
The conditions used to generate data for the resolution graph

above include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in
25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column
volume (CV); flow rate: 2.3 mL/min (13 cv/min); sample
loading: 4% of total binding capacity.
Applications
Contaminant removal such as DNA viral particles,
host cell proteins, or endotoxin
Acrodisc Unit with Mustang Q Membrane:

Dynamic Binding with BSA
Isolation via capture and release of plasmid DNA,

virus, or target protein from a complex mixture
1800
Elution Peak
1600
Protein (mg/mL)
1400
Protein fractionation or capture
1200
Antibody purification
1000
800
Reference material
600
54 mg/mL at 0
Breakthrough
400
200
Product Data, Acrodisc Unit with Mustang S Membrane,

PN 33256
0
0
10
15
20
25
Time (min)
Product Data, Acrodisc Unit with Mustang Q Membrane,

PN 33255
A solution of 0.524 mg/mL BSA was pumped through the

Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1
minutes and was calculated as 54 mg/mL using:
flow rate (2.3 mL/min) X initial protein BSA
concentration (0.524 mg/mL) X time (8.1 min)
membrane bed volume of Mustang Q membrane
in 25 mm Acrodisc unit (0.18 mL)
www.pall.com
11
Chromatography products selection guide

Chromatography Type
Product
Description
Particle Size
(Average)
Capacity
Primary Applications
Size Exclusion (Gel Filtration)

Separation by Molecule Size
Bulk Resin
Ultrogel AcA
Ultrogel AcA are polymeric resins for size exclusion composed

of polyacrylamide and agarose, characterized by narrow particle
size distribution.
100 m
N/A
Fractionation, purification of biomolecules

by size, molecular weight determination
Trisacryl GF05 M
Trisacryl GF are highly hydrophilic copolymer resins designed

for medium pressure gel filtration.
60 m
N/A
Lowest exclusion limit for desalting and other

small molecule removal
Trisacryl GF2000 LS
Trisacryl GF are highly hydrophilic copolymer resins designed

for medium pressure gel filtration.
120 m
N/A
Purification of macromolecules
Q Ceramic HyperD 20
Strong anion exchanger. Ceramic HyperD ion exchangers employ

a high capacity hydrogel polymerized within the large pores of
a rigid ceramic bead.
20 m
> 85 mg/mL (4)
Polypeptide and plasmid purification
S Ceramic HyperD 20
Strong cation exchanger. Ceramic HyperD ion exchangers employ

20 m
> 85 mg/mL (5)
Polypeptide purification
Q Ceramic HyperD F
Strong anion exchanger. Ceramic HyperD ion exchangers employ

50 m
> 85 mg/mL (4)
Recombinant proteins, monoclonal antibodies,

plasmid, vaccine purification, capture step
S Ceramic HyperD F
Strong cation exchanger. Ceramic HyperD ion exchangers employ

50 m
> 75 mg/mL (5)

vaccine purification, capture step
DEAE Ceramic
HyperD F
Weak anion exchanger. Ceramic HyperD ion exchangers employ

50 m
> 85 mg/mL (4)

plasmid, vaccine purification, capture step
CM Ceramic HyperD F
Weak cation exchanger. Ceramic HyperD ion exchangers employ

50 m
> 60 mg/mL (6)

vaccine purification, capture step
Q HyperZ
Q HyperZ are specifically designed for high productivity

expanded bed chromatography and efficient capture of biomolecules
directly from crude, unclarified samples in a single pass operation.
75 m
> 80 mg/mL (9)
Expanded bed and packed bed separations
CM HyperZ
CM HyperZ are specifically designed for high productivity

expanded bed chromatography and efficient capture of biomolecules
directly from crude, unclarified samples in a single pass operation.
75 m
~ 50 mg/mL (10)
Expanded bed and packed bed separations
Ion Exchange
Separation by Charge
Bulk Resin
Membrane Filter Plates and Devices

Mustang Q
Strong anion exchanger. Also available in AcroPrep 96 filter plates

350 L or 1 mL, and Acrodisc syringe filters.
N/A
50 - 60 mg/mL
Protein fractionation
Mustang S
Strong cation exchanger. Also available in AcroPrep 96 filter plates

350 L or 1 mL, and Acrodisc syringe filters.
N/A
45 - 50 mg/mL
Blue Trisacryl M
Blue Trisacryl M is an affinity chromatographic resin used for the

purification of a wide variety of enzymes and proteins such as kinases,
albumin, interferons and some coagulation factors. The basic matrix is
Trisacryl GF2000, a macroporous non-ionic resin on which Cibacron*
blue is covalently immobilized.
60 m
HSA: 10 - 15 mg/mL;
BSA: 5 - 7 mg/mL (1)
Albumin depletion
IMAC HyperCel
IMAC HyperCel uses tridentate IDA (imino-diacetic-acid) as a

chelating agent. The ligand is immobilized on the HyperCel
base sorbent, a stable and robust resin.
90 m
30 - 60 mol
Cu++/mL resin
Tagged biomolecule purification
Protein A Ceramic
HyperD F
Protein A Ceramic HyperD F is a high capacity affinity resin

prepared using a rigid proprietary ceramic bead. Recombinant
Protein A is immobilized to a specially formulated hydrogel within
the porous ceramic bead.
50 m
> 30 mg/mL (2)
IgG purification/depletion
Heparin HyperD M
Heparin HyperD M composite chromatography resin is used to purify

80 m
biological molecules that bind to heparin such as coagulation factors,
growth factors and lipoproteins. Heparin HyperD M is composed of a
porous rigid mineral bead containing heparin bound hydrogel filled pores.
> 25 mg/mL (3)
Purification of coagulation factors,

lipoproteins, growth hormones, growth
factors, nucleic acid binding enzymes
Lysine HyperD
Lysine HyperD is used to purify biological molecules that bind to lysine

such as glycoproteins. Lysine HyperD is comprised of a porous rigid
mineral bead containing lysine (L-lysine) bound hydrogel filled pores.
70 m
N/A
Purification of glycoproteins
Enchant Albumin
Depletion Kit
For the depletion of albumin from plasma or serum. Includes all

buffer and devices needed for 25 purifications.
N/A
> 2 mg albumin
per purification
Albumin depletion or isolation
Enchant Protein A Kit

for IgG Purification
For the purification of IgG. Includes all buffers and devices needed
for 50 purifications.
N/A
11 - 19 mg human
IgG depletion or purification
IgG/mL of gel, 6 - 8 mg
mouse IgG/mL of gel
Enchant Protein G Kit

for IgG Purification
For the purification of IgG. Includes all buffers and devices

needed for 10 purifications.
N/A
10 - 15 mg human
IgG/mL of gel
IgG depletion or purification
Enchant Multi-Protein
Affinity Separation Kit
For the removal of 99% of albumin and IgG from

serum/plasma samples.
N/A
> 99% removal
Albumin and IgG fractionation
Affinity
Separation Using
Specific Ligands
Bulk Resin
Kits
12
Chromatography Type
Product
Particle Size
(Average)
Description
Capacity
Primary Applications
Mixed Mode and Hydrophobic Charge Induction (HCIC)

A Unique Combination of
Chromatography Modes
Bulk Resin
MEP HyperCel
MEP HyperCel (4-mercapto-ethyl-pyridine) resin is specifically

designed for the capture and purification of monoclonal and polyclonal
antibodies. In contrast to Protein A resins, IgG binding on
MEP HyperCel is essentially independent of subclass or species.
Weakly binding variants (e.g., murine IgG, rat IgG) are well retained.
90 m
> 20 mg/mL (7)
Purification/depletion of polyclonal and

monoclonal antibodies of most species
SDR HyperD
SDR HyperD is a mixed mode of size exclusion, normal phase

and reversed phase. It is a unique resin designed to eliminate
solvent and detergent while recovering NATIVE protein. SDR HyperD
is a composite resin that combines a silica bead moiety filled with
long chain aliphatic polymers that are cross-linked to provide a 3D
mesh with a low size exclusion limit of 10 kDa which excludes proteins.
80 m
60 - 80 mg/mL (11)
Solvent and detergent removal
HA Ultrogel hydroxyapatite resin is composed of cross-linked

agarose beads with micro-crystals of hydroxyapatite entrapped
in the agarose mesh.
120 m
Cytochrome C:
> 7 mg/mL (8)
Immunoglobulin separation,
glycoproteins, vaccines
Hydroxyapatite
Protein Interaction with
Calcium Phosphate
Bulk Resin
HA Ultrogel
(1) capacity determined in PBS buffer using 5 mg/mL
(7) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in PBS, flow rate: 60 cm/h
(2) dynamic binding capacity, 10% breakthrough, 100 cm/h, determined using 10 mg/mL hu IgG in PBS, pH 7.4;
elution in 0.1 M sodium citrate, pH 2.5; column 4.6 ID x 100 mm
(8) capacity for cytochrome c, determined using 5 mg/mL cytochrome c diluted 50/50 in 1 mM phosphate buffer,
pH 6.8; at 12.5 cm/h
(3) dynamic binding capacity at 600 cm/h, using hu ATIII at 72.5 UI/mL in 20 mM Tris-HCl, 0.3 M NaCl, pH 7.4;
elution with 20 mM Tris-HCl, 2 M NaCl, pH 7.4; 10 cm bed height
(9) dynamic binding capacity, 10% breakthrough determined using 5 mg/mL BSA in 50 mM Tris-HCl buffer,
pH 8.6; 150 mM NaCl
(4) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL BSA in 50 mM Tris-HCl
buffer, pH 8.6
(10) dynamic binding capacity, 10% breakthrough, determined using 5 mg/mL hu IgG in 50 mM sodium acetate
buffer, pH 4.7; 150 mM NaCl
(5) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL lysosome in 50 mM sodium
acetate, pH 4.5
(11) dynamic binding capacity, 10% breakthrough at 300 cm/h, determined using 5 mg/mL Triton* in PBS, pH 7.4
(6) dynamic binding capacity, 10% breakthrough, 200 cm/h; sample: 5 mg/mL hu IgG in 50 mM sodium acetate,
100 mM NaCl, pH 7.4
Process proteomics
centers enhance service
The identification and optimization of protein purification
parameters can be a tedious task. Process proteomics
methodologies can be used to streamline the initial scouting
of protein purification conditions as well as for some of
the optimization steps. Process proteomics is performed
using common chromatographic chemistries (e.g., anion
exchange, cation exchange, IMAC, etc.) on a protein
chip, in the wells of a multi-well filter plate, or using small
columns. Using this approach, multiple binding, washing
and elution conditions can be tested on your sample
simultaneously. Successful scale-up from these small-scale
experiments to traditional column chromatography has
proven to be quite useful.
Pall's Process Proteomics Service Centers assist customers
in selecting and optimizing resins and membranes for the
purification of proteins used in the scale-up and production
of therapeutic proteins and other bioprocess applications.
With access to a large portfolio of both resin and media
technologies, Pall can provide highly integrated solutions
for our customers.
www.pall.com
13
Multi-well Filter Plates

Speed Your Rate of Discovery
As samples get smaller and more numerous, the need for
Palls filter plate product line includes two platforms that
novel methods to purify proteins and improve assays has
address different application needs:
led Pall to develop a broad line of multi-well filter plates

that target specific application challenges. AcroPrep
and AcroWell filter plates feature individually sealed
membranes that eliminate crosstalk and solution weeping.
The proprietary sealing technology allows us to seal virtually
any type of membrane or media configuration into a
device platform to meet ever-changing industry needs.
Special features
Broad Selection to Suit Your Application
AcroPrep filter plates are engineered with special outlet tips

and splash guards, and can be used for both filtrate- and
retentate-based applications. Membranes are individually
cut, placed and sealed in the wells using a proprietary
sealing process that ensures seal integrity. A distinctive
valve technology eliminates sample leaking.
AcroWell filter plates are designed to support retentate
and hybridization-based binding applications. These
plates are constructed of two membrane layers;
the bottom layer protects the upstream functional
membrane and acts as a barrier to passive flow.
The unique nature of protein science results in a variety

of device requirements for each application type. Pall
understands this need and has a full portfolio of 96- and
384-well filter plates that can be optimized for your assay.
Our portfolio includes a selection of single- and multi-layer
membranes, plate colors, well volumes, and outlet tips.
14
Using a plate optimized for your application will reduce

sample loss, make automation easy, and add consistency
and reliability throughout your entire process.
Automation Compliant
Palls plates are designed in accordance with the standards
of the ANSI/SBS X-2004. Rigid construction enables the
plates to be easily maneuvered by robotic instrumentation
and assures that the plates will seat properly on vacuum
manifolds, wash stations, hotel carousels and deck
platforms. Plates are compatible with industry leading
workstations including: Tecan, Qiagen Inc., Tomtec,
PerkinElmer Life and Analytical Sciences, Beckman Coulter
Inc., Caliper Life Sciences, Inc., Waters, Proteodyne, and
Hamilton Company.
No Crosstalk
Mass Spec Friendly, Chemically Resistant,

and Low Binding
Specially engineered fluid directors and outlet tips on the
The polypropylene housing assembly has been tested to
potential for downstream crosstalk. Elimination of crosstalk
ensure that the materials of construction do not contribute
upstream is assured through individually sealing a membrane
to ion suppression/enhancement. In addition, the housing
in each well using Palls proprietary sealing technology. Pall
materials and media have been optimized and tested to
understands the critical nature of each sample, and we
reduce extractables, ensuring that unwanted materials are
know that seal failure will cause sample loss. To ensure
not introduced to your sample. The housing is compatible
integral sealing of each well, we test each lot of product for
with a broad range of aqueous and organic liquids, and is
seal integrity prior to release. You can be assured that
noted for its low biomolecule binding that minimizes non-
AcroPrep and AcroWell filter plates will provide a robust
specific adsorption of samples to the plates.
platform that will eliminate concerns of sample loss and
bottom of the AcroPrep plate are designed to reduce the
cross contamination.
www.pall.com
15
The AcroPrep 96 filter plate shows consistent well-to-well

performance, giving protein biochemists an edge in the
development of protein purification protocols.
Efficient Optimization of Protein Purification

Conditions: AcroPrep 96 Filter Plates as
Mini-chromatography Columns
Although size separation applications can be done effectively
using ultrafiltration, a more specific affinity Immobilized Metal
Affinity Chromatography (IMAC) system is typically needed
to purify tagged biomolecules from crude lysates. Pall
has demonstrated the use of our multi-well filter plates to
perform high throughput IMAC. The low protein binding
and low weeping properties of the AcroPrep 96 filter plate
with low protein binding membrane are ideal for convenient
incubation of the sample directly in wells. The biomoleculefriendly AcroPrep 96 filter plate allows the researcher to
rapidly and reliably screen numerous samples under a
variety of conditions to determine protein purification
parameters. A single filter plate can be matrixed to:
Screen for metal ions for both custom and
pre-charged resins.
Optimize elution conditions.
Optimize resin-to-load ratio.
16
Incubation in
Centrifuge Tubes
Incubation in
AcroPrep 96 Plates
M L FT W1 W2 E1 E2 E3
M FT E1 E2 E3
Aliquots of Ni-NTA resin (Qiagen) were mixed with E. coli inclusion

body lysate containing a His-tagged TEV protease construct
(load). The slurry was either incubated in a microfuge tube and
then transferred to a filter plate (left panel) or incubated directly
in a well of an AcroPrep 96 filter plate with 0.2 m Bio-Inert
membrane (right panel). After washing, the samples were serially
eluted with either 3 X 200 L (left panel) or 3 X 50 L (right panel)
of elution buffer. The load (L), flow through (FT), wash (W1 and
W2), and elution (E1, E2, and E3) were analyzed by SDS-PAGE.
The incubation of sample/resin slurry directly in the wells of the
filter plate gave similar recoveries to those incubated in microfuge
tubes, allowing the simplification of sample handling. Based on
this observation, the on-plate incubation procedure was used
for the subsequent experiments.
Efficient Desalting and High Protein Recovery

Using AcroPrep 96 Filter Plates
The efficiency of AcroPrep 96 ultrafiltration filter plates to
remove salts and other small molecules does not diminish
sample recovery. Pall has demonstrated that when using
an AcroPrep 96 filter plate with 10K Omega membrane,
the recovery of ovalbumin proteins (45 kDa) at two different
concentrations (0.1 and 1.0 mg/mL) was greater than 90%.
Salt removal efficiencies were also greater than 95%. Using
BSA (66 kDa) and a 30K Omega membrane, similar protein
recoveries and desalting efficiencies were observed.
Applications
Bead-based applications
Lysate clarification
Protein concentration
Protein purification
Gross fractionation
Protein desalting
Size exclusion separations
Reference material
Sell Sheet, AcroPrep and AcroWell Multi-well,
Membrane-bottom Plates, PN 33287
Product Data, AcroPrep Membrane-bottom Plates, PN 33296
Product Data, AcroWell 96 Membrane-bottom Plates, PN 33306
AcroPrep 96 Filter Plate with 10K Omega Membrane

100
90
80
70
60
50
99.1
97.3
94
93
Ovalbumin
(45kDa)
0.1 mg/mL,
500 mM NaCl
Ovalbumin
(45kDa)
1.0 mg/mL
200 mM NaCl
40
30
20
10
0
% Salt Removed
100
98.1
94
95
90
80
BSA (66kDa)
0.1 mg/mL,
500 mM NaCl
70
60
50
40
BSA (66kDa)
1.0 mg/mL,
200 mM NaCl
30
Protocol, Lysate Clearance for Prokaryotic DNA Isolation

Using the AcroPrep 96 Filter Plate, PN 33308
Protocol, Automated Purification of Combinatorial Libraries
Using AcroPrep 96 Filter Plate with GHP Membrane, PN 33245
Protocol, Biomolecule Binding and Blocking Procedures for
AcroWell 96 Filter Plates with BioTrace NT and BioTrace
PVDF Membranes, PN 33189
Protocol, Using the AcroWell 96 Filter Plate for
Receptor/Ligand Binding, PN 33179
% Protein Recovered
AcroPrep 96 Filter Plate with 30K Omega Membrane

99.2
Protocol, Desalting/Buffer Exchange for Biomolecules Using

AcroPrep 96 Ultrafiltration Filter Plates, PN 33309
20
Technical Report, IMAC Purification of Polyhistidine-tagged

Protein Using the AcroPrep 96 Filter Plate, PN 33354
Technical Report, Automated Plate ELISA and Dot-Blot
Assays Using AcroWell 96 Filter Plates and a Robotic
Workstation with Integrated Plate Reader, PN 33293
Technical Report, Development of a Fluorescent LigandBinding Assay Using the AcroWell Filter Plate, PN 33220
Technical Report, The AcroWell 96 Filter Plate: Low Fluorescence Background Using the DELFIA* System, PN 33137
10
0
% Salt Removed
% Protein Recovered
300 L of indicated protein solutions was added to wells of 10K

or 30K plates. Each test plate was matched to a receiver plate and
the assembly spun at 2,000 x g for 40 min. Following centrifugation,
retained proteins were collected by adding 300 L of buffer to each
assay well, then allowing the plate to stand at room temperature for
5 min. before pipetting up and down 10 times to remove sample
to fresh tubes. Protein concentration was determined using UV
spectrophotometric analysis (n = 3). Percentage of salt removal
was determined using a conductivity meter. A representative
experiment is shown.
Technical Report, The AcroWell Filter Plate Minimizes

Crosstalk, see www.pall.com/proteomics
www.pall.com
17
Centrifugal Devices Facilitate Pure,

Concentrated Product with High Recoveries
Palls ultrafiltration centrifugal devices simplify many common
Palls microfiltration centrifugal devices are used in protein
protein handling procedures. These devices provide efficient
separation and small-scale general filtration procedures.
concentration and salt removal of samples from 50 L to
These devices can be used in combination with
60 mL in just minutes. Choose from membranes that have
chromatography resins to create a fast, efficient
been developed to assure low nonspecific biomolecule
method for purifying proteins of interest.
binding and provide consistent, high recovery of target

molecules. Ultrafiltration reduces the amount of handling
Special features
that can cause damage to samples, leaving concentrated
Rapid Processing
samples ready for direct incorporation into downstream
Achieve high recoveries in as little as five to ten minutes.
applications at critical stages in the discovery process.
High Performance Membranes

Omega polyethersulfone ultrafiltration membrane provides
higher flow rates and is lower protein binding than competitive
membranes. This results in lower processing time and the
highest possible recoveries.
Low Protein Binding
Devices are constructed of low-binding materials
to maximize sample recovery.
Variety of MWCOs and Pore Sizes
Available with ultrafiltration membranes for rapid concentrating
and/or desalting of proteins. Also available with low-binding
microfiltration membranes for particulate removal or
chromatography separations.
Easy to Use
Once you have identified the right MWCO or pore size ranging
from 1 kD to 0.45 m, the devices are color-coded for easy
visual identification.
Ideal for Fast Batch Mode
Chromatography Applications
Nanosep centrifugal devices with microfiltration membrane
serve as a perfect housing for chromatography resin. The spin
device can be filled with the resin of choice to perform the
desired protein purification application.
18
Nanosep Devices Exhibit Fast Spin Times and High Recoveries

MWCO
Spin Time (min.)
% Recovery
3K
15
7
10K
10
-
30K
8
-
100K
5
-
300K
3
-
6,200
% Recovery
99
51
11
12,400
% Recovery
100
89
77
1.8
Solute
Vitamin B12
Solute MW (Kd)
1,335
Aprotinin
Cytochrome C
Chymotrypsinogen A
25,000
% Recovery
97
94
2.1
Ovalbumin
45,000
% Recovery
97
92
BSA
67,000
% Recovery
100
26
1.5
Phosphorylase B
97,400
% Recovery
95
91
IgG
156,000
% Recovery
97
1.5
Thyroglobulin (1 mg/mL)
677,000
% Recovery
100
91
Match device size to sample volume
Samples of 0.5 mL of a
1.0 mg/mL solution were
centrifuged at 14,000 x g and
concentrated to a volume of 10 to
60 L using Nanosep centrifugal
devices with Omega membrane.
Applications
Palls centrifugal devices are available in a range of
Concentrate, purify and desalt peptides and proteins
sizes to accommodate your specific sample volumes.
Separate proteins from acrylamide gels
AcroPrep filter plates can be used with smaller sample
Prepare samples for HPLC analysis
volumes in similar applications.
Fractionate proteins
Device
AcroPrep 384 filter plate
AcroPrep 96 filter plate, 350 L
AcroPrep 96 filter plate, 1 mL
Nanosep device
Microsep device
Macrosep device
Jumbosep device
Sample Volume
< 100 L
< 350 L
< 1 mL
< 0.5 mL
0.5 - 3.5 mL
3 - 15 mL
15 - 60 mL
Easy-to-assemble stirred cell

systems process 2 to 150 mL
Palls Stirred Cell Systems
provide a versatile format that
can be disposed of when working
with biologically hazardous or
radioactive materials, or cleaned
and reused up to 20 times.
These devices are 100% integrity tested to ensure the
membrane and cell reservoir are integral. Ultrasonically
Reference material
Sell Sheet, Centrifugal Devices, PN 33327
Product Data, Centrifugal Devices for Ultrafiltration
and Microfiltration, PN 32984
Product Data, Stirred Cell Systems and Ultrafiltration
Membrane Disc Filters, PN 32985
Protocol, Desalting/Buffer Exchange for Biomolecules
Using AcroPrep 96 Ultrafiltration Filter Plates, PN 33309
Protocols, Nanosep Centrifugal Devices, PN 32989
Technical Report, Fast and Efficient Elution of Proteins from
Polyacrylamide Gels Using Nanosep Centrifugal Devices,
see www.pall.com/proteomics
Technical Report, Purification and Handling of DNA Fragments,
Technical Report, Nanosep Centrifugal Ultrafiltration Devices
and PCR: Before and After, see www.pall.com/proteomics
Technical Report, Single-tube DNA Purification and Cloning
Using Ultrafiltration Devices, see www.pall.com/proteomics
sealed membranes eliminate the need for O-rings that can

leak. The devices are easy to assemble, use and clean up,
and feature as much as 50% more effective filtration area
than conventional stirred cells of the same volume.
www.pall.com
19
Enchant Protein Purification

Kits Expedite Proteomic Sample Prep
Special features
Enchant Albumin Depletion or Purification Kit
Effectively process up to 100 L of serum or plasma
in 5 simple steps.
Remove > 2 mg of albumin per column.
Remove albumin from multiple species including human,
rat, goat, calf, and bovine.
Albumin can be discarded or recovered for further analysis.
Enchant IgG Depletion or Purification Kits
High binding. Bind between 11-19 mg of human
IgG/mL of gel (typical binding capacity).
Purify a variety of IgG molecules from a broad range of
species including human, horse, mouse, rat, cow, goat, etc.
Each column can be regenerated and used
for ten purifications.
The first step in isolating new drug targets is critical, and
Convenient kits contain all components necessary to

affinity purify or deplete IgG with either Protein A or
Protein G affinity resin from ascites fluid, serum or plasma.
reliable purification with minimal loss is key. Biospecific
IgG can be discarded or recovered for further analysis.
affinity ligand technologies such as Palls Enchant Protein A

or G antibody purification/depletion kits are widely used in
immunoglobulin purification for research and therapeutic
applications. Enchant Protein Purification Kits rapidly deplete
unwanted abundant proteins and unmask low abundant
biomarkers from human and animal-derived serum and
plasma samples. Alternately, these same kits can be used
to collect the abundant protein fraction for further analysis.
Convenient kit formats eliminate the need to handle messy
slurries or deal with column packing. These all-in-one kits
include the protocol, purification columns and buffers, and
offer one of the lowest costs per sample available.

Achieve 99% removal of albumin and IgG from
human serum or plasma samples.
High specificity. Will not remove low abundant,
low molecular weight biomarkers.
No loss of protein on the fractionation columns.
Can process up to 50 L human serum or plasma
in just 15 minutes.
Albumin and IgG fractions can be further fractionated
and analyzed.
Reference material
Sell Sheet: Enchant Albumin Depletion Kit, PN 33355
Sell Sheet: Enchant IgG Purification Kits, PN 33356
Product Data, Enchant Life Science Kits Albumin Depletion,
Product Data, Enchant Life Science Kits IgG Purification,
20
Effective Depletion of Human Serum Albumin (HSA)

and IgG from Plasma and Serum Samples
1
Versatile Purification and Depletion of IgG

and HSA from Multiple Species: Protein A Kit
1
5 6
7 8
IgG
IgG
HSA
IgG HC
IgG LC
Plasma
Serum
Depletion of both IgG and HSA using Enchant Abundant Protein

Depletion kits. One mL of human plasma was processed using the
Enchant Protein A IgG purification kit. Then, 30 L of the column
flow through was treated with the Enchant albumin depletion kit.
Samples were loaded onto 4-12% SDS-PAGE gels and resolved
in MOPS/SDS running buffer under non-reducing conditions.
Proteins were visualized with colloidal Coomassie* blue. Left panel
shows results for human plasma; right panel shows results for
human serum. Lane 1 in both panels shows starting material,
1 L. Lane 2 shows IgG removal from the Protein A column flow
through. Lane 3 shows subsequent removal of albumin.
Visualize Low Abundant Proteins with Effective

Albumin Depletion
Human Rabbit Mouse Rat
Purification of different species IgGs using Enchant Protein A IgG

Purification kit. One mL of plasma (human, rat) or serum (rabbit,
mouse) was processed according to insert instructions. The plasma
or serum starting material (1 L Lanes 1, 3, 5, 7) and one eluate
from each species with an A280 of approximately 1.0 (10 L Lanes
2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolved
under non-reducing conditions in MOPS/SDS running buffer. Proteins
were visualized with colloidal Coomassie blue staining. Efficient
depletion occurred with each species processed.
Versatile Purification and Depletion of IgG

and HSA from Multiple Species: Protein G Kit
1
5 6
7 8
IgG
Non-depleted
IgG HC
IgG LC
Depleted
Human Rabbit Mouse Rat
Two dimensional gel electrophoresis (2DGE) analysis of human

plasma following treatment using the Enchant Albumin Depletion kit.
Briefly, 20 L of human plasma was diluted with 30 L of binding
buffer and added to the Enchant albumin depletion column.
Sample was incubated for 10 minutes at room temperature then
spun at 12,000 x g for one minute. Filtrate was recovered, added
back to resin, incubated for 2 minutes and spun again. The
recovered filtrate and starting material were analyzed by 2DGE by
focusing in the first dimension on a pH 4-7 IPG strip then resolving
the second dimension on an 8-16% tris-glycine gel under reducing
conditions. Proteins were visualized with colloidal Coomassie blue.
Purification of different species IgGs using Enchant Protein G IgG

Purification kit. One mL of plasma (human, rat) or serum (rabbit,
mouse) was processed according to insert instructions. The plasma
or serum starting material (1 L Lanes 1, 3, 5, 7) and one eluate
from each species with an A280 of approximately 1.0 (10 L Lanes
2, 4, 6, 8) were loaded onto 4-12% SDS-PAGE gels and resolved
under non-reducing conditions in MOPS/SDS running buffer. Proteins
were visualized with colloidal Coomassie blue staining. Efficient
depletion occurred with each species processed.
www.pall.com
21
Increase Productivity
Using Tangential Flow Filtration
Special features
Easy Set Up and Use
Simply connect the TFF device to a pump and pressure
gauge(s), add sample, and process.
High Concentration Factors
Low hold-up volumes allow high concentration factors
to be achieved from small starting volumes.
Fast and Efficient Processing
Higher concentrations can be achieved in less time than with
centrifugal devices or stirred cells. Sample concentration and
diafiltration can be achieved on the same system, saving
time and avoiding product loss.
Scale Up or Down
Identical fluid path lengths and materials of
Tangential Flow Filtration (TFF) is a rapid and efficient
method for separating and purifying biomolecules. Palls
TFF products combine low protein binding ultrafiltration or
microfiltration membranes with optimized flow path design
to quickly concentrate samples while achieving high
concentration factors for sample volumes from 10 mL
construction allow precise linear scale-up to

larger systems. The membrane area of a
smaller device can be increased simply by
connecting multiple devices or adding cassettes.
Assuring predictable performance saves time when
scaling a process from pilot to production.
to thousands of liters. Systems are designed for easy set
22
up and use. By incorporating the same path length and
Economical
materials of construction throughout our TFF product line,
TFF devices and cassettes can be cleaned and reused, or
conditions established during pilot-scale trials can easily be
disposed of after a single use. A simple integrity test can be
applied to process-scale applications.
performed to confirm that membrane and seals are intact.
Optimize performance by
selecting the proper device
Choosing the appropriate cassette or device size depends
on the total sample volume, the required process time, and
the desired final sample volume. Performance parameters
for Palls laboratory TFF devices are presented below.
General Product Selection Based on Starting Sample Volume
Recommended
Retentate Flow Rate/
Capsule or Cassette
for Screen Channel
TFF Capsule
or Cassette*
Membrane Area/
Capsule or Cassette
Typical Filtrate
Flow Rate** at
50 LMH 20 C
Minimate
50 cm2 (0.05 ft2)
4 mL/min
LAB SCALE/SCALE-UP
30 - 80 mL/min
25 - 1000 mL
< 10 mL
LV Centramate
0.01 m2 (0.1 ft2)
8 mL/min
60 - 80 mL/min
40 - 2000 mL
10 mL
LV Centramate
0.02 m2 (0.2 ft2)
15 mL/min
120 - 160 mL/min
60 - 4000 mL
15 mL
Ultrasette
0.084 m2 (0.9 ft2)
0.2 - 5 L
100 mL
Centramate
0.093 m2 (1.0 ft2)
0.2 - 25 L
100 mL
PROCESS DEVELOPMENT AND SMALL-SCALE PRODUCTION

4 L/hr
1200 - 1500 mL/min
4.6 L/hr
* Data is per unit or cassette. Centramate holder can hold five

cassettes. Other column data can be calculated by multiplying
table values by the number of cassettes installed in the holder.
** Typical filtrate flow rate is based on an average filtrate flow rate
of 50 LMH and a process time of about four hours. Actual value
may be higher or lower depending on the MWCO of membrane,
600 - 800 mL/min
Starting Sample
Volume Range
Minimum
Concentrated
Volume***
sample composition and viscosity, operating conditions, i.e.,

transmembrane pressure, cross flow rate, temperature, etc.
*** Minimum concentrated volume depends on system hold-up
volume, reservoir design and pump type and speed. Smaller
volumes can be achieved by minimizing tubing lengths and
use of properly sized components, tubing, fittings, etc.
www.pall.com
23
Minimate TFF system

streamlines lab-scale
concentration, desalting, and
buffer exchange processes
The Minimate TFF system efficiently concentrates samples
from up to one liter to as little as 5 mL, enabling high
concentration factors. Subsequent desalting or buffer
exchange steps can be run on the same system with
minimal user intervention. The system works with Palls
Minimate TFF capsule, a disposable device designed to
accelerate and simplify scale-up applications.
24
Gain Precise Control of Protein Concentration

with the Minimate TFF System and a Liquid
Chromatography System
Applications
Concentration and desalting proteins and peptides
25
250
Sample preparation prior to or post chromatography

200
20
Reference material
Conductivity (mS/cm)
Absorbance (mAU)
Conductivity
150
15
A280
100
10
50
0
10
15
20
Data Sheet, Minimate Tangential Flow Filtration System

and Minimate TFF Capsule, PN 33366
Technical Report, Increased Productivity Using Minimate
Capsules to Replace Stirred Cell Systems, PN 33342
A600
5
Brochure, Improve Biopurification Processes, PN 33377-BIO
25
30
35
40
-50
Time (min)
The concentration of a 1 mg/mL BSA solution was accomplished

by leaving the diafiltration feed line open to air. The run, using a
Minimate 10K capsule, was processed at 25 mL/min recirculation
rate. Salt, protein (A280) and turbidity (A600) were monitored
using in-line sensors.
Facilitate Sequential Buffer Exchange and

Concentration with the Minimate Capsule
and a Liquid Chromatography System
Technical Report, Diafiltration: A Fast, Efficient Method for

Desalting or Buffer Exchange of Biological Samples, PN 33289
Minimate TFF Capsule Frequently Asked Questions, see

www.pall.com/proteomics
90
70
700
60
Conductivity
50
500
40
A280
300
30
Conductivity (mS/cm)
80
900
Absorbance (mAU)
Technical Report, Desalting and Buffer Exchange by Dialysis,

Gel Filtration or Diafiltration, PN 33290
Technical Report, Introduction to Tangential Flow Filtration for

Laboratory and Process Development Applications, PN 33213
100
1100
Technical Report, The Partnership of the Minimate TFF Capsule

with Liquid Chromatography Systems Facilitates Lab-scale
Purifications and Process Development Through In-line
Monitoring, PN 33339
20
100
A600
80
100
120
140
10
160
180
-100
200
220
240
260
Time (min)
Sequential diafiltration followed by concentration was documented

for a single run using a 1 mg/mL BSA solution in 1X PBS, 1M
NaCl starting solution. The run using a Minimate 10K capsule was
processed at 25 mL/min recirculation rate with the buffer exchange
from high salt to low salt. The diafiltration buffer feed line was then
opened to air, allowing the concentration of the sample. Salt,
protein (A280) and turbidity (A600) were monitored using
in-line sensors.
www.pall.com
25
Simplify Prefiltration
and Clarification Procedures
Although a basic filtration concept, the clarification and
prefiltration of samples remains an important function within
proteomics. When filtration is used as a prefilter, matching
the proper filter media and device to the application is
critical. Here, larger pore size filter materials are used to filter
solutions prior to more detailed analysis. When selecting the
best product for your application, numerous factors need to
be considered. Sample viscosity, sample volume and
sample recovery are just some of the aspects that will drive
the selection of the optimal device.
Pall offers a number of media and device options for fast,

effective filtration with minimal sample hold-up for both
single sample and high throughput processing. From
sample volumes of a few microliters to multiple liters, Pall
can supply the best product solution for your application.
Sample Viscosity
Device
VacuCap PF
Bottle-top Filters
4628
4638
Serum Acrodisc
Syringe Filters
4525
Acrodisc PF
Syringe Filters
AcroPrepTM 96
Filter Plates
Sample Volume
Acrodisc PSF
Syringe Filters
4524
AP-4523
4658
4187
5053
5046
5041
Recommended Part Numbers
26
Rapid, Effective Clarification of Plasmid

Purification Lysates Increases Recoveries
Built-in Prefilter Enhances Throughput of Viscous,

Particulate-laden or Proteinaceous Solutions
The use of filtration for the clarification of plasmid purification
The more particulate-laden and/or the higher the protein
lysates enables a rapid and effective alternative to centrifugal
concentration in a solution, the more difficult it is to filter.
sedimentation. The AcroPrep 96 filter plate with an integral
These types of liquids may clog filters prematurely. The
prefilter configuration improves the time and effectiveness
integration of a prefiltration media dramatically increases both
of filtration by allowing the prefiltration of viscous samples.
the throughput and flow rates of proteinaceous solutions.
The use of an integral prefilter shortens filtration times,

10
50
40
0.2 m
PF
equal to sedimentation.
12
Flow Rate (mL/sec)
10
6
4
2
00
8
PN 5042
PN 5046
COMP
6
4
B. diminuta (mL)
100% Calf Serum (mL)
allows greater flow rates, and results in high recoveries

30
20
10
5
10
Time (sec)
15
20
00
10
15
Time (sec)
20
Acrodisc and Acrodisc PF syringe filters with 0.2 m Supor

membrane were challenged with bovine serum or a bacterial
culture (107 cfu/mL) at a constant pressure of 1.4 bar
(140 kPa, 20 psi). The use of a prefilter (PF) device
significantly increased throughput and flow rate.
Reference material
0
100 L
200 L
Volume Filtered
Filtration times were measured for 100 L and 200 L lysate

samples. Average flow rates were calculated for the AcroPrep 96
filter plate with Bio-Inert membrane (PN 5042), AcroPrep 96 filter
plate with Glass Fiber prefilter over Bio-Inert membrane (PN 5046),
and a Competitor hydrophilic plate containing a floating prefilter
(COMP). Error bars indicate standard error (n=8).
For the complete protocol see, Lysate Clearance for
Brochure, Improve Biopurification Processes, PN 33377-BIO

Product Data, Sterile Acrodisc Syringe Filters, PN 33175
Product Data, VacuCap and VacuCap PF Vacuum
Filtration Devices, PN 32914
Product Data, AcroPrep Membrane-bottom Filter Plates,
PN 33296
Technical Report, Syringe Filter Efficiency and Effect
of Filtration on HPLC Column Life, PN 33312
Prokaryotic DNA Isolation Using the AcroPrep 96 Filter

Plate, PN 33308.
www.pall.com
27
Obtain High Sensitivity

for Protein Detection
For protein characterization, the use of membrane technology is ideal for obtaining detailed structural information.
Pall offers an impressive range of membranes compatible
with all common protein detection procedures. Our
membranes feature superior binding capacities and
extremely low background to facilitate the transfer and
retention of rare and low-abundant proteins for detection
Gain sensitive, detailed

structural information
FluoroTrans Membrane has Excellent Sensitivity,
Signal, and Extremely Low Background in
Western Transfers
FluoroTrans
membrane
FluoroTrans W
membrane
Competitor
PVDF membrane
and further characterization.

Palls strict quality manufacturing specifications ensure
consistent, precise membrane performance from
lot to lot and blot to blot. Our membranes set industry
standards for reproducible results, durability, and high
signal-to-noise ratios. Choose from a range of commonly
used formats including rolls, discs, sheets, or custom cuts.
PVDF Membranes
FluoroTrans, FluoroTrans W, and BioTrace PVDF
membranes are hydrophobic in nature and strongly bind
Rabbit reticulocyte lysate (GE Healthcare) was loaded in

lanes of polyacrylamide gels at full strength, 1/3 and 1/10 dilutions.
After electrophoresis, proteins were transferred to membranes.
Membranes were stained with 0.1% Amido Black, 45% methanol,
2% acetic acid for 4 minutes and were then destained for
5 minutes with two changes of 90% methanol, 2% acetic
acid. Stained membranes were rinsed in water and air dried.
proteins of interest. FluoroTrans membrane is recommended

for protein sequencing and is compatible with all reagents
involved in the Edman reaction. FluoroTrans W and BioTrace
PVDF membranes exhibit low background and high tensile
strength, and are the best membranes available for detection
of protein after Western transfer. All FluoroTrans membranes
exhibit exceptionally low burn through.
Nitrocellulose Membranes
BioTrace NT pure nitrocellulose membrane can be used
for Western transfers and ELISpot assays. The membrane
offers high protein binding and is compatible with a wide
range of protein stains and immunodetection techniques.
Activated Membranes
Pall offers two activated membranes for covalent protein binding: UltraBind modified polyethersulfone has a high ratio of
covalent to non-covalent protein binding; Immunodyne ABC
membrane offers the sharp spot geometry of nylon with a
proprietary covalent attachment chemistry.
28
Avoid membrane damage,

make filter handling easy
Palls forceps feature flat, smooth tips that gently handle
membrane filters. Polypropylene finger grips provide a
comfortable and secure hold. Choose traditional black
or multi-colored finger grips for forceps that are easy
to identify, track, and see on the lab bench.
AcroWell 96 filter plates

expand detection potential
Applications
Western transfers
For protein detection in a multi-well format, AcroWell 96 filter
N-terminal protein sequencing
plates are excellent for parallel or automated applications.
ELISA
Choose plates with BioTrace NT (nitrocellulose) or PVDF
Affinity separation
membrane. See pages 14 17 for more information on
ELISpot Assays
filter plates.
Reference material
Direct fluorescent detection
with Vivid microarray slides
Product Data, Membranes for Transfer and Immobilization,

PN 33082
Vivid Microarray Slides exhibit good signal to noise and
Product Data, AcroWell 96 Membrane-bottom Filter Plates,

PN 33306
dose response along with excellent sensitivities when used

for double antibody type assays. Easy protocols, simple
immobilization steps, and automation-friendly design make
Vivid slides the ideal choice for consistent, detectable results.
High Sensitivity Double Antibody Assay
for HSR Using Vivid Microarray Slides
pg/spot
1,250
625
312
156
78
39
20
10
1st antibody: Mouse

anti-human HSA.
2nd antibody: Goat
anti-mouse IgG
conjugated to APC.
Eight dilutions of HSA
were printed onto a Vivid
Gene Array slide using
a Genomic Solutions
G3 robot; 4x4 blocks;
4 blocks per dilution.
Spots are visible at
the lowest level
printed (10 pg).
Protocol, Double Antibody Nanoimmunoassay with Direct

Fluorescent Detection, PN 33273
Protocol, Biomolecule Binding and Blocking Procedures for
AcroWell 96 Filter Plates with BioTrace NT and BioTrace
PVDF Membranes, PN 33189
Protocol, Transfer and Detection Procedures for Pall Life
Sciences Membranes, PN 33167
Technical Report, Automated Plate ELISA and Dot-Blot
Assays Using AcroWell 96 Filter Plates and a Robotic
Workstation with Integrated Plate Reader, PN 33293
www.pall.com
29
Ordering Information
Chromatography resins
(lab scale volumes)
Trisacryl
Blue
M Affinity
Chromatography Resin
Part Number
Description
Packaging
25896-051
Blue Trisacryl M
5 mL
25896-045
Blue Trisacryl M
25 mL
25896-010
Blue Trisacryl M
100 mL
25896-028
Blue Trisacryl M
1000 mL
Spherodex LS
Silica/Dextran
DEAE and SP
Composite Ion Exchange Resins
Part Number
Description
Packaging
26455-023
DEAE Spherodex LS
100 g
20080-024
SP Spherodex LS
100 mL
Ultrogel
HA
Hydroxyapatite
Part Number
Description
Packaging
24775-075
HA Ultrogel Hydroxyapatite
5 mL
24775-082
25 mL
24775-025
100 mL
24775-041
1000 mL
Heparin
M Affinity
Part Number
Description
Packaging
20029-062
Heparin HyperD M
5 mL
20029-039
Heparin HyperD M
25 mL
20029-021
Heparin HyperD M
100 mL
20029-013
Heparin HyperD M
1000 mL
IMAC HyperCel Chromatography Resin

Part Number
Description
Packaging
20093-069
IMAC HyperCel
5 mL
20093-010
IMAC HyperCel
25 mL
IMAC HyperCel
100 mL
Lysine HyperD Chromatography Resin

Part Number
Description
Packaging
20059-058
Lysine HyperD
5 mL
20059-036
Lysine HyperD
25 mL
20059-028
Lysine HyperD
100 mL
20059-010
Lysine HyperD
1000 mL
MEP HyperCel Hydrophobic Charge

Induction Chromatography (HCIC) Resin
Part Number
Description
Packaging
12035-069
MEP HyperCel
5 mL
12035-010
MEP HyperCel
25 mL
12035-028
MEP HyperCel
100 mL
12035-036
MEP HyperCel
1000 mL
Methyl Ceramic HyperD F

30
Trisacryl GF Size Exclusion

Part Number
Description
Packaging
Part Number
Description
Packaging
20078-036
Protein A Ceramic HyperD F
5 mL
25914-060
Trisacryl GF05 M
100 mL
20078-028
25 mL
25914-037
Trisacryl GF05 M
1000 mL
20078-010
100 mL
25916-040
Trisacryl GF05 LS
100 mL
20078-044
1000 mL
25916-016
Trisacryl GF05 LS
1000 mL
26064-055
Trisacryl GF2000 M
100 mL
26064-022
Trisacryl GF2000 M
1000 mL
26065-045
Trisacryl GF 2000 LS
100 mL
26065-011
Trisacryl GF 2000 LS
1000 mL
SDR HyperD Solvent-Detergent

Removal Chromatography Resin
Part Number
Description
Packaging
20033-065
SDR HyperD
5 mL
20033-031
SDR HyperD
25 mL
20033-023
SDR HyperD
100 mL
Ultrogel AcA Size Exclusion

20033-015
SDR HyperD
1000 mL
Part Number
Description
Packaging
23013-025
Ultrogel AcA 22
100 mL
Q and CM HyperZ Ion Exchange Resins
23013-014
Ultrogel AcA 22
1000 mL
Part Number
Description
Packaging
23015-025
Ultrogel AcA 34
100 mL
21012-010
Q HyperZ
50 g
23015-019
Ultrogel AcA 34
1000 mL
21012-020
Q HyperZ
250 g
23022-024
Ultrogel AcA 44
100 mL
21012-030
Q HyperZ
1 kg
23022-015
Ultrogel AcA 44
1000 mL
21011-010
CM HyperZ
50 g
23019-023
Ultrogel AcA 54
100 mL
21011-020
CM HyperZ
250 g
23019-011
Ultrogel AcA 54
1000 mL
21011-030
CM HyperZ
1 kg
24892-022
Ultrogel AcA 202
100 mL
24892-010
Ultrogel AcA 202
1000 mL
Q, S, DEAE, CM Ceramic HyperD,

CM Trisacryl M Ion Exchange Resins
HyperD
20093-028
Protein A Ceramic HyperD F Affinity

Part Number
Description
Packaging
20051-033
25 mL
20051-025
100 mL
20051-071
1000 mL
Part Number
Description
Packaging
Ion exchange
chromatography devices
20040-051
Q Ceramic HyperD 20
5 mL
20040-044
Q Ceramic HyperD 20
25 mL
20040-036
Q Ceramic HyperD 20
100 mL
AcroPrep 96 Filter Plates, 350 L Well
20040-028
Q Ceramic HyperD 20
500 mL
Part Number
Description
Packaging
20040-010
Q Ceramic HyperD 20
1000 mL
5047
Mustang Q membrane, natural
10/pkg
20038-055
S Ceramic HyperD 20
5 mL
5048
Mustang S membrane, natural
10/pkg
20038-048
S Ceramic HyperD 20
25 mL
20038-030
S Ceramic HyperD 20
100 mL
AcroPrep 96 Filter Plates, 1 mL Well
20038-022
S Ceramic HyperD 20
500 mL
Part Number
Description
Packaging
20038-014
S Ceramic HyperD 20
1000 mL
5062
Mustang Q membrane, natural
5/pkg
20066-098
Q Ceramic HyperD F
5 mL
5063
Mustang S membrane, natural
5/pkg
20066-031
Q Ceramic HyperD F
25 mL
20066-023
Q Ceramic HyperD F
100 mL
Acrodisc Units, Mustang Membranes
20066-015
Q Ceramic HyperD F
1000 mL
Part Number
Description
Packaging
20062-089
S Ceramic HyperD F
5 mL
MSTG25Q6
10/pkg
20062-030
S Ceramic HyperD F
25 mL
20062-022
S Ceramic HyperD F
100 mL
Acrodisc unit with Mustang Q

membrane, 0.8 m, 25 mm,
(blister non-sterile packs)
20062-014
S Ceramic HyperD F
1000 mL
MSTG25S6
Acrodisc unit with Mustang S

membrane, 0.8 m, 25 mm,
(blister non-sterile packs)
10/pkg
20067-070
5 mL
20067-039
25 mL
20067-021
100 mL
20067-013
1000 mL
20050-084
CM Ceramic HyperD F
5 mL
20050-035
CM Ceramic HyperD F
25 mL
20050-027
CM Ceramic HyperD F
100 mL
20050-019
CM Ceramic HyperD F
1000 mL
26708-016
CM Trisacryl M
300 mL
Multi-well filter plates

Part Number
Description
Packaging
5033
3K Omega membrane, natural
10/pkg
5034
10/pkg
5035
10/pkg
5036
10/pkg
5045
0.2 m GHP membrane, natural
10/pkg
5030
0.45 m GHP membrane, natural 10/pkg
5043
0.45 m GHP membrane, white
10/pkg
5044
0.45 m GHP membrane, black
10/pkg
5037
0.2 m PTFE membrane, natural 10/pkg
5038
0.45 m PTFE membrane,

natural
10/pkg
5042
0.2 m Bio-Inert membrane,

natural
10/pkg
5046
3.0 m glass fiber media/0.2 m 10/pkg

Bio-Inert membrane, natural
5031
1.0 m glass fiber, natural

1.0 m glass fiber, white
10/pkg
5029
0.45 m Supor membrane,

natural
10/pkg
5039
1.2 m Supor membrane, natural 10/pkg
5041
Prefilter material/1.2 m
Supor membrane, natural
10/pkg
Mustang Q ion exchange

membrane, natural
10/pkg
Mustang S ion exchange

membrane, natural
10/pkg
5048
5049
54 m screen, natural
10/pkg
AcroPrep 96 Filter Plates, 1 mL Well

Part Number
Description
Packaging
5052
0.2 m GHP membrane, natural
5/pkg
5054
0.45 m GHP membrane, natural 5/pkg
5055

natural
5/pkg
5056

natural
5/pkg
5051
1.0 m glass fiber media,

natural
5/pkg
3.0 m glass fiber

media/0.2 m Bio-Inert
membrane, natural
5/pkg
Mustang Q ion exchange

membrane, natural
5/pkg
Mustang S ion exchange

membrane, natural
5/pkg
5053
5062
5063
Part Number
Description
5020
0.45 m GHP membrane, natural, 10/pkg

350 L well
Packaging
0.45 m GHP membrane, white,

350 L well
10/pkg
5022
0.2 m BioTrace NT
membrane, white
10/pkg
5025
0.2 m BioTrace NT membrane,

black
10/pkg
5023
0.45 m BioTrace PVDF

membrane, natural
10/pkg
5026

membrane, black
10/pkg

membrane, white
10/pkg
5027
Description
Packaging
5076
10K Omega membrane,

long tips, natural
10/pkg
Part Number
Description
Packaging
ODM02C33
0.2 m, aqua
24/pkg
10K Omega membrane,

short tips, natural
10/pkg
ODM02C34
0.2 m, aqua
100/pkg
5078
30K Omega membrane,

long tips, natural
10/pkg
ODM02C35
0.2 m, aqua
500/pkg
ODM45C33
0.45 m, wildberry
24/pkg
5079
30K Omega membrane,

short tips, natural
10/pkg
ODM45C34
0.45 m, wildberry
100/pkg
ODM45C35
0.45 m, wildberry
500/pkg
5080
100K Omega membrane,

long tips, natural
10/pkg
5081
100K Omega membrane,

short tips, natural
10/pkg
5070
0.45 m GHP membrane,

long tips, natural
10/pkg
5071
0.45 m GHP membrane,

short tips, natural
5072
5077
Nanosep MF Centrifugal Devices,

GHP Membrane
Part Number
Description
Packaging
ODGHPC34
0.45 m, clear
100/pkg
10/pkg
ODGHPC35
0.45 m, clear
500/pkg

long tips, natural
10/pkg
Microsep Centrifugal Devices,

Omega Membrane
5072W

long tips, white
10/pkg
5073

short tips, natural
10/pkg
5073W

short tips, white
10/pkg
5084

long tips, natural
10/pkg

short tips, natural
10/pkg
Part Number
Description
Packaging
OD001C41
1K, yellow
24/pkg
OD001C46
1K, yellow
100/pkg
OD003C41
3K, gray
24/pkg
OD003C46
3K, gray
100/pkg
OD010C41
10K, blue
24/pkg
OD010C46
10K, blue
100/pkg
OD030C41
30K, red
24/pkg
OD030C46
30K, red
100/pkg
Vacuum Manifold
OD050C41
50K, green
24/pkg
Part Number
Description
OD050C46
50K, green
100/pkg
5017
Multi-well plate vacuum manifold 1/pkg
OD100C41
100K, clear
24/pkg
5014
1 mL receiver plate spacer block
1/pkg
OD100C46
100K, clear
100/pkg
5015
350 L receiver plate

spacer block
1/pkg
OD300C41
300K, orange
24/pkg
OD300C46
300K, orange
100/pkg
5016
Replacement accessory kit

(includes O-ring, gasket,
allen wrench)
1/pkg
OD990C41
1000K, purple
24/pkg
OD990C46
1000K, purple
100/pkg
5085
Packaging
Microsep MF Centrifugal Devices,

Bio-Inert Membrane
Accessories
Part Number
Description
Packaging
5225
Adapter collar for centrifugation
2/pkg
5230
96-well plate cap mats
5/pkg
5231
Multi-well plate lids
10/pkg
Centrifugal devices
Part Number
Description
Packaging
ODM02C67
0.2 m, aqua
24/pkg
ODM02C68
0.2 m, aqua
100/pkg
ODM45C67
0.45 m, wildberry
24/pkg
ODM45C68
0.45 m, wildberry
100/pkg
Macrosep Centrifugal Devices,

Omega Membrane
Nanosep
AcroWell 96 Filter Plates, 350 L Well
5021
Part Number
Nanosep MF Centrifugal Devices,

Bio-Inert Membrane
10/pkg
5032
5047
Centrifugal Devices,
Omega Membrane
Part Number
Description
Packaging
1K, yellow
6/pkg
24/pkg
Part Number
Description
Packaging
OD001C36
OD003C33
3K, gray
24/pkg
OD001C37
1K, yellow
OD003C34
3K, gray
100/pkg
OD001C38
1K, yellow
100/pkg
3K, gray
6/pkg
OD003C35
3K, gray
500/pkg
OD003C36
OD010C33
10K, blue
24/pkg
OD003C37
3K, gray
24/pkg
OD010C34
10K, blue
100/pkg
OD003C38
3K, gray
100/pkg
OD010C35
10K, blue
500/pkg
OD010C36
10K, blue
6/pkg
10K, blue
24/pkg
OD030C33
30K, red
24/pkg
OD010C37
OD030C34
30K, red
100/pkg
OD010C38
10K, blue
100/pkg
OD030C35
30K, red
500/pkg
OD030C36
30K, red
6/pkg
24/pkg
OD030C37
30K, red
24/pkg
OD030C38
30K, red
100/pkg
OD100C33
100K, clear
OD100C34
100K, clear
100/pkg
OD100C35
100K, clear
500/pkg
OD300C33
300K, orange
24/pkg
OD300C34
300K, orange
100/pkg
OD300C35
300K, orange
500/pkg
PTFE plates have polyester support.

Glass Fiber plates have polypropylene support.
www.pall.com
31
Centrifugal devices
Macrosep Centrifugal Devices,
Omega Membrane
Part Number
Description
Packaging
OD050C36
50K, green
6/pkg
OD050C37
50K, green
24/pkg
OD050C38
50K, green
100/pkg
OD100C36
100K, clear
6/pkg
OD100C37
100K, clear
24/pkg
OD100C38
100K, clear
100/pkg
OD300C36
300K, orange
6/pkg
OD300C37
300K, orange
24/pkg
OD300C38
300K, orange
100/pkg
OD990C36
1000K, purple
6/pkg
OD990C37
1000K, purple
24/pkg
OD990C38
1000K, purple
100/pkg
Jumbosep Centrifugal Device Starter Kits
Stirred cell systems
Enchant protein
purification kits
10 mL Omega Membrane-cell Packs

Part Number
Description
Packaging
Enchant Life Science Kit Albumin Depletion
OC001C30
1K, yellow
10/pkg
Part Number
Description
Packaging
OC003C30
3K, gray
10/pkg
OC005C30
5K, tan
10/pkg
5300ALBDEP
Enchant Albumin Depletion

Kit (25 Nanosep 0.45 m
GHP centrifugal devices,
25 Nanosep filtrate
tubes, 25 albumin-depleting
discs, 6.25 mL binding/
wash buffer)
25
samples
Part Number
Description
Packaging
FD000K65
Generic starter kit,

(no membrane inserts)
4/pkg
OC010C30
10K, blue
10/pkg
FD003K65
3K starter kit, gray
4/pkg
OC030C30
30K, red
10/pkg
FD010K65
10K starter kit, blue
4/pkg
OC050C30
50K, green
10/pkg
FD030K65
30K starter kit, red
4/pkg
OC100C30
100K, clear
10/pkg
FD100K65
100K starter kit, clear
4/pkg
OC300C30
300K, orange
10/pkg
FD300K65
300K starter kit, orange
4/pkg
Jumbosep Centrifugal Device

Membrane Inserts
Enchant Life Science Kits IgG Purification
150 mL Omega Membrane-cell Packs
Part Number
Description
Part Number
Description
Packaging
OC001C60
1K, yellow
5/pkg
5300IGGPROA
Enchant Protein A IgG

50
Purification Kit (5 Protein A
purifications
affinity purification columns,
5 desalting columns, 1 liter
Protein A binding buffer,
500 mL Protein A elution buffer)
5300IGGPROG
Enchant Protein G IgG

10
Purification Kit (1 Protein G
purifications
affinity purification column,
5 desalting columns, 240 mL
Protein G binding buffer,
120 mL Protein G elution buffer)
Part Number
Description
Packaging
OC003C60
3K, gray
5/pkg
OD003C65
3K membrane insert, gray
12/pkg
OC005C60
5K, tan
5/pkg
OD010C65
10K membrane insert, blue
12/pkg
OC010C60
10K, blue
5/pkg
OD030C65
30K membrane insert, red
12/pkg
OC030C60
30K, red
5/pkg
OD100C65
100K membrane insert, clear
12/pkg
OC050C60
50K, green
5/pkg
OD300C65
300K membrane insert, orange
12/pkg
OC100C60
100K, clear
5/pkg
OC300C60
300K, orange
5/pkg
Packaging
Jumbosep Device Accessory Products

Part Number
Description
Packaging
Stirred Cell Generic Starter Kits
FD001X65
Filtrate receiver and cap
12/pkg
Part Number
Description
Packaging
Part Number
Description
Packaging
FD002X65
Sample reservoir and cap
12/pkg
FC000K30
10 mL Generic Starter Kit
1 kit
FD003X65
Insert release
24/pkg
FC000K60
150 mL Generic Starter Kit
1 kit
5300AFFMPS
Enchant Multi-Protein Affinity

Separation Kit (24 Nanosep
centrifugal devices, 5 mL
anti-HSA resin, 5 mL
anti-IgG resin, 20 mL wash
buffer, 20 mL elution buffer)
24
purifications
Omega Membrane Discs (25, 43, 47, 50, 62, and 76 mm provided 12/pkg; 90 and 150 mm provided 6/pkg)
32
MWCO
25 mm
43 mm
47 mm
50 mm
62 mm
76 mm
90 mm
150 mm
1K
OM001025
OM001043
OM001047
OM001050
OM001062
OM001076
OM001090
OM001150
3K
OM003025
OM003043
OM003047
OM003050
OM003062
OM003076
OM003090
OM003150
5K
OM005025
OM005043
OM005047
OM005050
OM005062
OM005076
OM005090
OM005150
10K
OM010025
OM010043
OM010047
OM010050
OM010062
OM010076
OM010090
OM010150
30K
OM030025
OM030043
OM030047
OM030050
OM030062
OM030076
OM030090
OM030150
50K
OM050025
OM050043
OM050047
OM050050
OM050062
OM050076
OM050090
OM050150
100K
OM100025
OM100043
OM100047
OM100050
OM100062
OM100076
OM100090
OM100150
300K
OM300025
OM300043
OM300047
OM300050
OM300062
OM300076
OM300090
OM300150
LV Centramate Cassettes, Alpha Membrane
Tangential flow
filtration products
Minimate TFF Capsules,
Omega Membrane
Medium
Screen
Suspended
Screen
MWCO
EFA
10K
0.01 m2 (0.1 ft2) AS010C12P1
AS010C11P1
10K
0.02 m2 (0.2 ft2) AS010C12P2
AS010C11P2
Description
Packaging
OAD65C12
650D
1/pkg
OA001C12
1K
1/pkg
Pore Size
EFA
Medium
Screen
OA003C12
3K
1/pkg
0.1 m
0.01 m2 (0.1 ft2)
PSM10C12P1 PSM10C11P1
OA005C12
5K
1/pkg
0.1 m
0.02 m2 (0.2 ft2)
OA010C12
10K
1/pkg
0.2 m
0.01 m2 (0.1 ft2)
OA030C12
30K
1/pkg
0.2 m
0.02 m2 (0.2 ft2)
OA050C12
50K
1/pkg
0.45 m
0.01 m2 (0.1 ft2)
OA070C12
70K
1/pkg
0.45 m
0.02 m2 (0.2 ft2)
OA100C12
100K
1/pkg
0.65 m
0.01 m2 (0.1 ft2)
OA300C12
300K
1/pkg
0.65 m
0.02 m2 (0.2 ft2)
OA500C12
500K
1/pkg
0.8 m
0.01 m2 (0.1 ft2)
OA990C12
1000K
1/pkg
0.8 m
0.02 m2 (0.2 ft2)
Part Number
Description
88216
1/pkg
Minimate Fitting Kit*
Consists of male luer to 3.2 mm
(1/8 in.) hose barb, female luer
to 3.2 mm (1/8 in.) hose barb,
3.2 mm (1/8 in.) i.d. tubing,
tubing screw clamp, tubing
clamps, adhesive strips
(loop and hook)
Part Number
Description
Packaging
OAPMP110
115V AC 50/60 Hz
1/pkg
OAPMP220
230V AC 50/60 Hz
1/pkg
OAPMP220UK
230V AC 50/60 Hz
with UK plug
1/pkg
Minimate TFF Reservoir Assembly
Pore Size
Medium
Screen
EFA
1/pkg
1K
0.01
OS001C12P1
OS001C11P1
Part Number Description
1K
0.02 m2 (0.2 ft2)
OS001C12P2
OS001C11P2
FS007K10
1/pkg
3K
0.01 m2 (0.1 ft2)
OS003C12P1
OS003C11P1
3K
0.02 m2 (0.2 ft2)
OS003C12P2
OS003C11P2
5K
0.01 m2(0.1 ft2)
OS005C12P1
OS005C11P1
5K
0.02 m2 (0.2 ft2)
OS005C12P2
OS005C11P2
10K
0.01 m2 (0.1 ft2)
OS010C12P1
OS010C11P1
10K
0.02 m2 (0.2 ft2)
OS010C12P2
OS010C11P2
30K
0.01 m2 (0.1 ft2)
OS030C12P1
OS030C11P1
30K
0.02 m2 (0.2 ft2)
OS030C12P2
OS030C11P2
2-gauge fitting package

consists of (2) 1/4 in. NPT
nipples, (2) 1/4 in. NPT tees,
(2) 1/4 in. NPT to 1/4 in.
barbed fittings, (1) 1/4 in. NPT
to 1/4 in. barbed elbow fitting,
(2) PSIG glycerin-filled gauges,
(1) 1/4 in. ID tubing, (2) 1/4 in.
NPT to 1/2 in. barbed fittings,
(5) stainless steel hose clamps,
and (1) 1/4 in. NPT to 1/4 in.
barbed tee fitting
50K
0.01 m2 (0.1 ft2)
OS050C12P1
OS050C11P1
FS008K10
0.02 m2 (0.2 ft2)
OS050C12P2
OS050C11P2
70K
0.01 m2 (0.1 ft2)
OS070C12P1
OS070C11P1
70K
0.02 m2 (0.2 ft2)
OS070C12P2
OS070C11P2
OS100C11P1
3-gauge fitting package

consists of (4) 1/4 in. NPT
nipples, (4) 1/4 in. NPT tees,
(3) 1/4 in. NPT to 1/4 in.
barbed fittings, (2) 1/4 in. NPT
to 1/4 in. barbed elbow fittings,
(3) PSIG glycerin-filled gauges,
(1) 1/4 in. ID tubing, (2) 1/4 in.
NPT to 1/2 in. barbed fittings,
and (5) stainless steel
hose clamps
1/pkg
50K
(0.1
0.01 m2 (0.1 ft2)
OS100C12P1
OARES110
Reservoir Assembly
115V AC 50/60 Hz
1/pkg
100K
0.02 m2 (0.2 ft2)
OS100C12P2
OS100C11P2
300K
0.01 m2 (0.1 ft2)
OS300C12P1
OS300C11P1
Reservoir Assembly
230V AC 50/60 HZ
1/pkg
300K
0.02 m2 (0.2 ft2)
OS300C12P2
OS300C11P2
500K
0.01 m2 (0.1 ft2)
OS500C12P1
OS500C11P1
Reservoir Assembly
230V AC 50/60 HZ
with UK plug
1/pkg
500K
0.02 m2 (0.2 ft2)
OS500C12P2
OS500C11P2
1000K
0.01 m2 (0.1 ft2)
OS990C12P1
OS990C11P1
1000K
0.02 m2 (0.2 ft2)
OS990C12P2
OS990C11P2
LV Centramate TFF Holder and Accessories
0.16 m
0.01 m2 (0.1 ft2)
OS994C12P1
OS994C11P1
Part Number
Description
0.16 m
0.02 m2 (0.2 ft2)
OS994C12P2
OS994C11P2
FS003K10
LV Centramate cassette holder 1/pkg
FS007X01
Bronze nuts and washers
4/pkg
Centramate Cassette Holders
FS710M01
Pressure gauge assembly

[2 needed (filtrate and
retentate); 3 needed if
monitoring required]
1/pkg
Part Number
Description
Packaging
FS001K10
Includes stainless steel

Centramate cassette holder,
assorted fittings, torque
wrench, and socket
1/pkg
FS002K10
Includes polyethylene holder

1/pkg
with Centramate cassette,
stainless steel top and bottom
brace plates, assorted fittings,
torque wrench, and socket
Male:male connector to attach 8/pkg

pressure gauges to holder
3-gauge fitting package consists

of (10) 1/2 in. EPDM gaskets,
(3) 1-1/2 in. EPDM gaskets,
(3) 1/2 in. x 1-1/2 in. TC tees,
(3) 1/2 in. TC to 1/4 in. ID tube
barbed fittings, (13) 1/2 in. TC
clamps, (2) 1/2 in. diaphragm
valves, (1) filtrate manifold,
(3) 0-60 PSIG glycerin-filled
gauges, (3) 3/4 in. TC to 1/2 in.
ID tube barbed fittings, and
(3) 1-1/2 in. TC sanitary clamps
Centramate PE Fitting Packages
100K
FS700X10
FS006K10
ft2)
m2
Packaging
Packaging
1/pkg
Suspended
Screen
Description
OARES220UK
2-gauge fitting package consists

of (8) 1/2 in. EPDM gaskets,
(2) 1-1/2 in. EPDM gaskets,
(2) 1/2 in. x 1-1/2 in. TC tees,
(3) 1/2 in. TC to 1/4 in. ID tube
barbed fittings, (8) 1/2 in. TC
clamps, (1) 1/2 in. diaphragm
valve, (1) filtrate manifold,
(2) 0-60 PSIG glycerin-filled
gauges, (3) 3/4 in. TC to 1/2 in.
ID tube barbed fittings, and
(2) 1-1/2 in. TC sanitary clamps
Suspended
Screen
Part Number
OARES220
Packaging
FS005K10
LV Centramate Cassettes, Omega Membrane
Packaging
Minimate TFF Systems
Part Number Description
LV Centramate Cassettes, Supor Membrane
Part Number
Minimate TFF Capsule Accessory Product
Centramate Sanitary Gauge Fitting Packages
Packaging
*One Minimate TFF accessory kit is included

in each Minimate TFF capsule package.
www.pall.com
33
Ultrasette Device Packages,
Omega Membrane
Tangential flow
filtration products
Centramate Cassette Hardware Systems
Screen Channel
Suspended
Screen
Ultralab Systems with 115 V Pump
MWCO
Part Number
Description
Packaging
1K, yellow
OS001C70
OS001C72
FS006X75
2 L Ultralab system
consists of an Ultrareservoir
container, Masterflex* L/S*
variable speed peristalic
pump, and Ultrasette
accessory kit; connects
to Ultrasette device
sold separately
1/pkg
FS007X70
5 L Ultralab system
container, Masterflex L/S
pump and Ultrasette
sold separately
1/pkg
Part Number
Description
Packaging
3K, gray
OS003C70
OS003C72
FS010K10
Centramate system,
2-gauge includes
PN FS001K10 and FS005K10
1/pkg
5K, tan
OS005C70
OS005C72
10K, blue
OS010C70
OS010C72
FS011K10
Centramate system,
3-gauge includes
1/pkg
30K, red
OS030C70
OS030C72
50K, green
OS050C70
N/A
70K, brown
OS070C70
N/A
100K, clear
OS100C70
OS100C72
300K, orange
OS300C70
OS300C72
Centramate PE Cassette Hardware Systems

Part Number
Description
Packaging
FS012K10
Centramate PE system,
2-gauge includes
1/pkg
FS013K10
Centramate PE system,
3-gauge includes
1/pkg
Device packages include (1) device in

the MWCO of your choice, (2) storage
caps for feed/retentate, filtrate outlet cap,
(2) tubing clamps, and 0.6 m (24 in.)
of 4.8 mm (3/16 in.) tubing.
Centramate Cassettes, Omega Membrane

MWCO
Fine
Screen
Medium
Screen
Suspended
Screen
1K
OS001C10
OS001C12
OS001C11
3K
OS003C10
OS003C12
OS003C11
5K
OS005C10
OS005C12
OS005C11
10K
OS010C10
OS010C12
OS010C11
30K
OS030C10
OS030C12
OS030C11
50K
OS050C10
OS050C12
OS050C11
70K
OS070C10
OS070C12
OS070C11
100K
OS100C10
OS100C12
OS100C11
300K
N/A
OS300C12
OS300C11
500K
N/A
OS500C12
OS500C11
1000K
N/A
OS990C12
OS990C11
0.16 m
N/A
OS994C12
OS994C11
Ultrasette Device Accessory Products

Part Number
Description
Packaging
FS002X70
Accessory kit consists of

1.8 m (6 ft.) of PharMed*
#24 feed/retentate tubing,
0.6 m (24 in.) of 4.8 mm
(3/16 in.) Tygon filtrate
tubing, (8) stainless steel
tubing clamps, (1) screw
clamp, and (1) barbed fitting
1/pkg
Gauge fitting package

consists of (1) 0 - 4.1 bar
(0 - 60 psi) 3.2 mm (1/8 in.)
NPT pressure gauge,
(2) 6.4 mm (1/4 in.) O.D.
polypropylene barbed tube
to 3.2 mm (1/8 in.) NPT
connectors, (1) 3.2 mm
(1/8 in.) threaded
polypropylene tee,
(1) screw clamp,
(2) stainless steel
tubing clamps
1/pkg
FS005X70
Centramate Cassettes, Supor Membrane

Pore Size
Medium
Screen
Suspended
Screen
0.03 m
PSM03C12
PSM03C11
0.1 m
PSM10C12
PSM10C11
0.2 m
PSM20C12
PSM20C11
0.45 m
PSM45C12
PSM45C11
0.65 m
PSM65C12
PSM65C11
0.8 m
PSM80C12
PSM80C11
Centramate Cassettes, Alpha Membrane
34
MWCO
Fine
Screen
Medium
Screen
Suspended
Screen
10K
AS010C10
AS010C12
AS010C11
FS001X70
Mounting bracket,
1/pkg
holds Ultrasette device
securely during operation;
suction cups on the bottom
of the bracket allow for
placement on smooth surfaces
Ultralab Systems with 230 V Pump

Part Number
Description
Packaging
FS016X75
2 L Ultralab system
container, Masterflex L/S
sold separately
1/pkg
FS017X70
5 L Ultralab system
container Masterflex L/S
sold separately
1/pkg
Ultrareservoir Containers
Part Number
Description
FS005X75
2 L container includes
1/pkg
0 - 4.2 bar pressure gauge,
4.0 mm fittings, 6.4 mm fittings,
and 3-way valve; suitable for
use with Ultrasette device
Packaging
FS006X70
5 L container includes
1/pkg
use with Ultrasette device;
fittings supplied are 6.4 mm
FS007X75
500 mL container includes

1/pkg
use with LV Centramate system
Biodyne B Membrane, 0.45 m
Prefiltration and
clarification
Acrodisc PF Syringe Filters,
Supor Membrane
Part Number
Description
Packaging
4187
0.8/0.2 m, 25 mm, sterile
50/pkg
4658
50/pkg
Acrodisc PSF GxF Syringe Filter, Glass Fiber

Part Number
Description
Packaging
AP-4523
GXF/Glass, 25 mm,
non-sterile
50/pkg,
200/cs
Serum Acrodisc Syringe Filter,

Supor Membrane
Part Number
Description
Packaging
60202
82 mm discs
50/pkg
60203
85 mm discs
50/pkg
60204
132 mm discs
50/pkg
60205
137 mm discs
50/pkg
60201
7 x 8.5 cm sheets
10/pkg
60200
20 x 20 cm sheets
10/pkg
60209
20 cm x 1 m roll
1/pkg
60208
20 cm x 3 m roll
1/pkg
60207
30 cm x 3 m roll
1/pkg
Biodyne C Membrane, 0.45 m

Part Number
Description
Packaging
60316
82 mm discs
50/pkg
60317
85 mm discs
50/pkg
Part Number
Description
Packaging
60318
132 mm discs
50/pkg
4525
GF/0.2 m, 37 mm, sterile
20/pkg
60319
137 mm discs
50/pkg
60315
7 x 8.5 cm sheets
10/pkg
Acrodisc Syringe Filter, Glass Fiber
60314
20 x 20 cm sheets
10/pkg
Part Number
Description
Packaging
Part Number
Description
Packaging
60251
29 cm x 3 m roll
1/pkg
66594
7 x 8.5 cm sheets
10/pkg
4524
1 m (nominal), 37 mm,
non-sterile
15/pkg,
60/cs
Biodyne Plus Membrane, 0.45 m
VacuCap 60 PF Device,
Supor Membrane
Part Number
Description
Packaging
60402
82 mm discs
50/pkg
60403
85 mm discs
50/pkg
BioTrace PVDF Transfer Membrane
66542
20 x 20 cm sheets
10/pkg
66547
20 cm x 1 m roll
1/pkg
66543
30 cm x 3 m roll
1/pkg
UltraBind Affinity Membrane
Part Number
Description
Packaging
60404
132 mm discs
50/pkg
Part Number
Description
Packaging
4638
10/pkg
60405
137 mm discs
50/pkg
66544
20 x 20 cm sheets
10/pkg
60401
7 x 8.5 cm sheets
10/pkg
66545
30 cm x 3 m roll
1/pkg
60400
20 x 20 cm sheets
10/pkg
60406
30 cm x 3 m roll
1/pkg
VacuCap 90 PF Device,
Supor Membrane
Part Number
Description
Packaging
4628
10/pkg
Protein detection
products
BioTrace NT Nitrocellulose Transfer

Membrane
Part Number
Description
Packaging
NBCH3RI
0.45 m, 30 cm x 3 m roll
1/pkg
NNCH3RI
1.2 m, 30 cm x 3 m roll
1/pkg
Part Number
Description
Packaging
66487
82 mm discs
50/pkg
66595
85 mm discs
50/pkg
Part Number
Description
Packaging
5110
Vivid Gene Array slides

with modified Nylon
10/pkg
5111
Vivid Gene Array slides

with modified Nylon
20/pkg
66518
132 mm discs
50/pkg
66488
137 mm discs
50/pkg
Packaging
66593
7 x 8.5 cm sheets
10/pkg
Biodyne A Membrane
Part Number
Description
60113
0.2 m, 30 cm x 3 m roll
1/pkg
66489
20 x 20 cm sheets
10/pkg
60102
0.45 m, 82 mm discs
50/pkg
66485
30 cm x 3 m roll
1/pkg
60103
0.45 m, 85 mm discs
50/pkg
60104
0.45 m, 132 mm discs
50/pkg
Part Number
Description
Packaging
60105
0.45 m, 137 mm discs
50/pkg
Part Number
Description
Packaging
5022
BioTrace NT membrane, white
10/pkg
60101
0.45 m, 7 x 8.5 cm sheets
10/pkg
PVM020C-160
7 x 8.4 cm sheets
10/pkg
5025
BioTrace NT membrane, black
10/pkg
10/pkg
PVM020C-195
8.5 x 9 cm sheets
20/pkg
FluoroTrans Membrane
AcroWell 96 Filter Plates,

BioTrace NT Membrane, 350 L
60100
0.45 m, 20 x 20 cm sheets
60120
0.45 m, 20 cm x 3 m roll
1/pkg
PVM020C1015
10 x 15 cm sheets
10/pkg
60106
0.45 m, 30 cm x 3 m roll
1/pkg
PVM020C-196
13 x 14 cm sheets
10/pkg
AcroWell 96 Filter Plates,

BioTrace PVDF Membrane, 350 L
60108
1.2 m, 30 cm x 3 m roll
1/pkg
PVM020C2020
20 x 20 cm sheets
10/pkg
Part Number
Description
PVM020C-099
26 cm x 3.3 m roll
1/pkg
5023
BioTrace PVDF membrane, natural 10/pkg
5026
BioTrace PVDF membrane, black
10/pkg
5027
BioTrace PVDF membrane, white
10/pkg
FluoroTrans W Membrane
Part Number
Description
Packaging
BSP0158
7 x 9 cm sheets
10/pkg
BSP0157
10 x 15 cm sheets
10/pkg
BSP0159
20 x 20 cm sheets
10/pkg
BSP0161
26 cm x 3.3 m roll
1/pkg
Packaging
www.pall.com
35
On-demand Support & In-demand Rewards

Proteomics Scientific and
Educational Resource Center
www.pall.com/proteomics
Palls online resource center offers a complete library of
technical resources, including technical protocols and
product information, for all aspects of proteomic research.
Visit us today to see how Pall Life Sciences can help
streamline your proteomics workflow.
Pall Preferred Program

www.pall.com/preferred
More than you expect. Just what you deserve. Join our
online community for access to members-only benefits
and promotions. Membership is free, registration is quick
and easy, and the rewards are extraordinary!
Pall LIfe Sciences

600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520 toll free in USA
734.665.0651 phone
734.913.6114 fax
Australia Lane Cove, NSW

Tel: 02 9424-3000
1800 635-082 (in Australia)
Fax: 02 9428-5610
Austria Wien
Tel: 00 1 49 192 0
Fax: 00 1 49 192 400
Canada Ontario
Tel: 905-542-0330
800-263-5910 (in Canada)
Fax: 905-542-0331
Canada Qubec
Tel: 514-332-7255
800-435-6268 (in Canada)
Fax: 514-332-0996
800-808-6268 (in Canada)
China P. R., Beijing
Tel: 86-10-8458 4010
Fax: 86-10-8458 4001
France St. Germain-en-Laye
Tel: 01 30 61 39 92
Fax: 01 30 61 58 01
Lab-FR@pall.com
Germany Dreieich
Tel: 06103-307 333
Fax: 06103-307 399
Lab-DE@pall.com
India Mumbai
Tel: 91 (0) 22 55995555
Fax: 91(0) 22 55995556
Italy Milano
Tel: 02 47 79 61
Fax: 02-47796 394
or 02-4122 985
Japan Tokyo
Tel: 03-6901-5800
Fax: 03-5322-2134
Korea Seoul
Tel: 82-2-560-7871
Fax: 82-2-560-7822
Malaysia Selangor
Tel: +60 3 5569 4892
Fax: +60 3 5569 4896
New Zealand Hamilton
Tel: +64 7 957 9510
Fax: +64 7 957 9513
Poland Warszawa
Tel: 22 510 2100
Fax: 22 510 2101
Russia Moscow
Tel: 5 01 787 76 14
Fax: 5 01 787 76 15
Singapore
Tel: (65) 389-6500
Fax: (65) 389-6501
South Africa
Johannesburg
Tel: +27-11-2662300
Fax: +27-11-3253243
Spain Madrid
Tel: 91-657-9876
Fax: 91-657-9836
Sweden Lund
Tel: (0)46 158400
Fax: (0)46 320781
Switzerland Basel
Tel: 061-638 39 00
Fax: 061-638 39 40
Taiwan Taipei
Tel: 886 2 2545 5991
Fax: 886 2 2545 5990
Thailand Bangkok
Tel: 66 2937 1055
Fax: 66 2937 1066
United Kingdom
Farlington
Tel: 02392 302600
Fax: 02392 302601
Lab-UK@europe.pall.com
Visit us on the Web at www.pall.com/lab

E-mail us at Lab@pall.com
, Acrodisc, AcroPrep, AcroWell, Alpha, Biodyne, Bio-Inert, BioSepra,
2006 Pall Corporation. Pall,
BioTrace, Centramate, Enchant, Fluorodyne, FluoroTrans, HyperCel, HyperD, HyperZ, Immunodyne,
Jumbosep, Leukosorb, LoProdyne, Macrosep, Microsep, Minimate, Mustang, Nanosep, Omega, Pallflex,
Repel, Spherodex, Supor, Trisacryl, Tuffryn, UltraBind, Ultralab, Ultrareservoir, Ultrasette, Ultrogel, VacuCap,
Versapor, and Vivid are trademarks of Pall Corporation. indicates a registered trademark in the USA.
is a service mark of Pall Corporation. *DELFIA is a registered trademark
of PerkinElmer, Inc. Triton is a registered trademark of Rohm & Haas Company. Coomassie is a registered
trademark of Imperial Chemicals Industries, Ltd. PharMed is a registered trademark of Norton Company.
L/S and Masterflex are registered trademarks of Cole Parmer Instrument Company. Cibacron is a registered
trademark of CIBA-Geigy Ltd.
7/06, PDF, GN05.1256 V2
PN 33411

05.1256 Accelerate Protein Sample Prep

Caricato da

Informazioni sul documento

Copyright

Formati disponibili

Condividi questo documento

Condividi o incorpora il documento

Opzioni di condivisione

Hai trovato utile questo documento?

Questo contenuto è inappropriato?

Copyright:

Formati disponibili

05.1256 Accelerate Protein Sample Prep

Caricato da

Copyright:

Formati disponibili

www.pall.

Accelerate Protein Sample

The results of my work can change peoples lives.

When we need support, Pall has the

product development opportunities that we use to benefit

Whether you are processing a single sample or detecting

solve complex customer problems, test products, explore

construction to allow precise scale-up of processing

With over 50 years of experience, Pall knows how to

Palls high throughput products meet industry guidelines

a range of applications. Our goal is to provide you with the

Addressing the Challenges of

Sample complexity reduction is an important first step to facilitate access to

Ceramic HyperD Ion Exchange Chromatography Resins

Trisacryl, HyperD, HyperCel Affinity Chromatography Resins

SDR HyperD Solvent-Detergent Removal Resins

MEP HyperCel Hydrophobic Charge Induction

fractionation technologies prior to 2D-Gel or LC-MS/MS separation. This can

Mustang Ion Exchange Membrane Devices

be achieved with a combination of specific affinity ligand-based depletion and

Omega Ultrafiltration Membrane for Size Exclusion

ion exchange chromatography-based fractionation technologies. Pall provides

MULTI-WELL FILTER PLATES (Pages 14 - 17)

separation technologies and devices to address these challenging needs in

AcroPrep Plates with Hydrophilic Filtration Membranes

both low and high throughput applications.

AcroPrep Plates with Ion Exchange Membranes

Sample preparation...its the last thing I want to worry

ARRAYS (Pages 13, 29)

In combination with appropriate chromatography resin.

Finding the Perfect Fit

Versatile separation technologies

Reliable device configurations

your application performance. Our quality control procedures

While the heart of Pall is media development and

result in the production of media with exceptional lot-to-lot

manufacturing, the design and manufacture of devices

reproducibility and uniformity to give you the consistent,

is the ultimate expression of Pall technologies. This is

accurate results you require.

where it all comes together high performance media,

Acrylic Copolymer Proprietary

Acrylic Copolymer Repel Treated

BioSepra Size Exclusion

Fractionation, purification, desalting

BioSepra Q Ion Exchange

Protein concentration, protein fractionation,

BioSepra S Ion Exchange

BioSepra DEAE Ion

BioSepra CM Ion Exchange

Filtration of protein solutions, low protein binding,

Filtration of low concentration proteins,

Time-resolved fluorescence, bead-based assays,

Filtration of low concentration proteins,

Prefilters, DNA extraction

Proven performance in documented procedures

Vent filters, chemical and molecular synthesis

Sample preparation, PCR cleanup, sequencing

BioSepra Affinity Resins

Abundant protein removal, lgG purification,

BioSepra Hydrophobic Charge

Cellulose Polymer 4-Mercaptoethyl-pyridine

Purification of poly and monoclonal antibodies of

Removes endotoxin from buffers, water, neutral