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Materials:
Procedure:
Trial I
Use fifty pennies placed tails-side up. Have your partner time you for ten
seconds as you try to turn as many as you can from tails-side up to headsside up. At the end of the first ten-second time period, record the number of
pennies you turned in the Data Table and then DO NOT put them back in the
pile of 50. You will continue turning the remaining pennies in ten second
intervals. Each time, make sure to record your results in the table and DO
NOT return the pennies to the pile. You are finished when you have turned
all 50 pennies from tails-side up to heads-side up.
Trial II
Reset all of the pennies by turning all of them tails side up. Use the tape to
bind the four fingers on your turning hand together. This represents partial
denaturation of the enzyme. Repeat the procedure from Trial I by turning the
pennies one by one in ten second intervals. You may need to add more tensecond periods if you still have pennies to turn. Make sure you record your
data in the Trial II column of your data table after each 10 second period.
Trial III
Reset all of the pennies by turning all of them tails side up. During this trial,
you will have the help of a partner, representing the role of a coenzyme. Your
partner will pick them up the pennies and give them to you and you will be
responsible for turning them head side up. This last process by the helper
may extend beyond the 10 second interval. This means that if your helper
has a penny in their hand when time expires, you may turn it over and count
it as completed within the time period. As before, six time periods will be
used and data recorded into the data table.
Trial IV
In this last trial you will retrieve pennies as you did in Trial I, but you will be
handicapped by taping of a tennis ball or some other object to the palm of
the hand to be used. Obviously, this object may interfere with your ability to
pick up the pennies. The ball or object represents an inhibitor which is
competing with the place on your hand where you pick up the pennies. Try it
for six time periods and see what happens. Record your data after each time
period.
Data Table
Time Periods
(seconds)
Trial I
(# of pennies)
Trial II
(# of pennies)
Trial III
(# of pennies)
Trial IV
(# of pennies)
0-10
10-20
20-30
30-40
40-50
50-60
60-70
70-80
Analysis:
Make a graph to show the number of pennies turned over per time period for
each trial (I-IV). Use a different color for each trial and be sure to label each
axis and series appropriately.
1. In this activity, what was the enzyme represented by? the substrate?
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