Curva de calibrado-ferroso

Fe mg.L-1
Abs (510 nm)
0.8
100
0.69
90
0.619
0.6
80
0.563
70
0.497
0.4
Abs (510 nm)
60
0.429
50
0.394
0.2

f(x) = 0.00689x + 0.
R = 0.996019148

40
0.29
0
30
0.204
0
20
40
20
0.147
in fe
10
0.08
0
0
Curva de calibrado - hierro total
Fe mg.L-1
Abs (510 nm)
0.8
100
0.67
90
0.601
0.6
f(x) = 0.006695454
80
0.531
R = 0.9974257685
70
0.461
0.4
60
0.394Abs (510 nm)
50
0.321
0.2
40
0.23
0
30
0.196
0
20
40
20
0.136
10
0.067
hierro
0
0

f(x) = 0.00689x + 0.0112272727

R = 0.996019148

20

40

60

80

100

120

100

120

in ferroso (mg/L)

f(x) = 0.0066954545x - 0.0068636364

R = 0.9974257685

20

40

60

80

hierro total (mg/L)

Fe+3
0
0
0
0
0
0
0
0

Con sales
Sol. A
tubo
1
2
3
4
5
6
7

Eh (mV)
382
391
414
465
521
563
578

Fe+2
987
977.3
888.3
493.5
98.7
9.87
0

Fe+3
18.15
17.97
16.335
9.075
1.815
0.18
0

mg/L
Sol. A
Sol. B
Total
m
E
Sol. Problema
Fe+3/Fe+2 Mta

Fe+2
987
11.07
998.07
54.844
472.93

Fe+3
18.15
861.56
879.71

452
-0.38162789
0.682749064
600
f(x) = 54.8443173898x + 472.9295938298
R = 0.9970133073

550

500

450

Eh

400

350

300
-2

-1.5

-1

-0.5

log (Fe+3

Sol. B
Fe+2
0
0.1107
1.107
5.535
9.9963
10.96
11.07

mg/L

Fe+3
0
8.616
86.156
430.78
775.404
852.94
861.56

Fe+2
987
977.4107
889.407
499.035
108.6963
20.83
11.07

Relacin
Fe+3
18.15
26.586
102.491
439.855
777.219
853.12
861.56

Fe+3/Fe+2
0.0183890578
0.0272004389
0.1152352073
0.8814111235
7.1503721838
40.9563130101
77.8283649503

600

550

500

450

400

350

300
0

0.5

log (Fe+3/Fe+2)

1.5

2.5

Relacin
Log(Fe+3/Fe+2)
-1.7354405233
-1.5654240887
-0.9384148127
-0.054821473
0.8543286478
1.6123208535
1.8911379067

Tiempo (h)
1
2
3
4
5
6
7
8

Eh
300
340
380
420
460
500
540
580

100
90
80
70

Fe+3/Fe+2

60
50
40
30
20
10
0
0

Tiempo (h
2.5

Log(Fe+3/Fe+2)
-3.1531252279
-2.4237838232
-1.6944424185
-0.9651010138
-0.2357596091
0.4935817956
1.2229232004
1.9522646051

Fe+3/Fe+2
0.0007028696
0.0037689136
0.0202095937
0.108367483
0.5810859716
3.1158876914
16.7079512857
89.5910455738

Tiempo (h)

Concentracin
0
0.06
0.12
0.18
0.24
0.3

Abs.
0
0.106
0.307
0.533
0.68
0.823

m
b

2.8871
-0.0249

Factor de dilucin

0.9
f(x) = 2.8871428571x - 0.0249047619
R = 0.9913906085

0.8

0.7

0.6

0.5
Abs.
0.4

0.3

0.2

0.1

0
0

0.05

0.1

0.15
Pr

.0249047619

0.1

0.15
0.2
Proteinas (g/L)

0.25

0.3

0.35

0.35

abs = a ( Fe mg/L) + b

Tiempo (h)
R1
0
2.5
5
7.5
10
12.5
15
17.5
20
22.5
25
27.5
30
32.5

Ferroso

a
b

0.0069 Fet
0.0112

Ferroso
R2
0.442
0.371
0.251
0.323

0.442
0.471
0.451
0.423

Hierro total
R2
R1
0.492
0.392
0.482
0.38
0.482
0.33
0.48
0.453

0.234
0.25
0.201
0.121
0.11
0.108
0.108
0.08
0.07

0.334
0.25
0.17
0.121
0.101
0.108
0.09
0.08
0.001

0.389
0.31
0.38
0.312
0.29
0.48
0.501
0.41
0.399

R1

0.434
0.523
0.46
0.537
0.481
0.314
0.326
0.405
0.404

Eh
344
372
381
382
387
396
407
428
447
482
537
603
610
620

a
b

0.0067
-0.007

Eh
R2

Dilucion:

130

cel/ml pH
344
372
381
382
387
396
407
428
447
482
537
603
610
620

###
###
###
###
###
###
###
###
###
###
###
###
###

1.55
1.42
1.43
1.55
1.57
1.59
1.68
1.73
1.78
1.81
1.8
1.89
1.88
1.78

120000000
100000000
80000000
60000000
40000000
20000000
0

0.90755705
5
0.18151141

10

15

20

15

20

25

30

35

Tiempo (h)
R1
0
3
17
19
21
23
37
39.5
42
44.5
47
49.5
52
54.5
57
59.5
62
64.5
67
69.5
dilucion

Ferroso
R2
0.738
0.727
0.532
0.643
0.741
0.711
0.412
0.548
0.319
0.472
0.395
0.35
0.263
0.263
0.182
0.045
0.073
0.018
0.05
333.3

0.698
0.7
0.71
0.651
0.472
0.538
0.581
0.404
0.578
0.346
0.419
0.457

Hierro total
R2
0.756
0.754
0.676
0.682
0.663
0.698
0.648
0.766
0.676
0.741
0.698
0.421

0.729
0.779
0.66
0.739
0.725
0.748
0.704
0.559
0.594
0.647
0.412
0.845

340
346
366
366
374
378
385
410
418
424
429
428

0.454
0.259
0.182
0.145
0.073
0.018
0.01

0.748
0.791
0.659
0.527
0.518
0.706
0.605

0.62
0.584
0.659
0.727
0.718
0.506
0.605

445
448
490
493
522
599
610

R1

Eh
R1

Eh
R2

cel/ml pH
340
350
369
372
377
380
384
413
413
420
425
436

###
###
###
###
###
###
###

459
467
469
524
563
588
610

###
###
###
###
###
###
###

###
###
###

1.68
1.53
1.82
1.88
1.86
1.95
1.99
1.68
1.57
1.61
1.61
1.79
1.53
1.63
1.51
1.54
1.47
1.49
1.49
1.5

0
Tiempo
0
3
17
19
21
23
37
39.5
42
44.5
47
49.5
52
54.5
57
70
100
130
160
170

1
1
3.13862317
3.55003021
3.11813605
3.97651668
4.11213154
3.68534666
4.53057463
4.84926781
4.80961142
4.65309893
4.83608346
3.88570689
4.98765467
5.5966906
5.48049212
5.87863933
5.48909371
5.10403265
5.16376282
6.5343599

2
4.21321386
1.5231303
4.11813605
5.97651668
2.11213154
5.68534666
3.53057463
5.84926781
6.80961142
3.65309893
5.83608346
5.88570689
2.98765467
7.5966906
5.88049212
6.78639332
7.1
6.10403265
4.16376282
5.5343599

Tiempo
0
3
17
19
21
23
37
39.5
42
44.5
47
49.5
52
54.5
57
70
100
130
160
170

1
3.13862317
4.55003021
4.11813605
4.97651668
6.11213154
6.68534666
10.5305746
10.8492678
10.8096114
10.6530989
10.8360835
12.8857069
12.9876547
14.5966906
14.4804921
14.8786393
18.4890937
22.1040326
30.1637628
29.5343599

3
2
3.87654321
5.76432118
5.32132345
5.81231268
7.90876541
7.87654467
11.3435679
13.8565789
12.6789081
11.8765437
11.3456789
13.5678909
13.4567891
13.876579
15.6789048
13.6789088
17.7890489
23.6789004
30.6789016
31.9087655

0
3
9
11
17
21
26
31
49
51
60
75
120
125

1
3.13862317
4.55003021
4.91813605
4.47651668
6.01213154
8.18534666
8.53057463
11.8492678
14.8857069
15.9876547
19.5966906
21.4804921
30.8786393
31.98765

2
2.45653211
3.5675435
5.76549181
5.89047652
7.98765012
7.54321853
7.90530575
12.7890849
13.5431219
16.7890988
20.7890597
22.2431311
31.7654568
32.5678977

0
2
6
10
15
29
21
29
41
58
66
90
100
106

8
1
3.13862317
4.55003021
4.91813605
4.47651668
6.01213154
8.18534666
8.53057463
8.84926781
13.8857069
17.9876547
20.5966906
27.4804921
30.8786393
31.98765

2
4.90876139
5.90767568
5.89091814
5.89047652
7.01213154
6.87618535
8.41232131
8.73432145
12.4123886
15.4512377
22.4213597
26.2131235
31.5238786
32.5432988

0
2
6
9
14
17
27
23
37
51
74
88
100
105

1
3.13862317
4.55003021
4.91813605
4.47651668
6.01213154
8.18534666
8.53057463
8.84926781
13.8857069
17.9876547
25.5966906
28.4804921
34
34.2345679

2
4.87678139
3.6789055
5.78913605
5.78951668
5.89012132
9.43231219
9.63432531
8.93243249
15.5432189
16.1212399
21.1259669
27.4214214
32.3542343
31.5432223