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pr
ob
abi
lity
Intracellular
15 mM NaCl
140 mM KCl
1.5 mM MgATP
10 mM pH buffer
(pH adjusted to 7.4)
-10
-100 mV
-100
0.5
0
0
-10
1F. (10 pts) Though satisfied with the success of your initial experiments, you tried to repeat
them again as any good
engineers had
-100scientist
mV would. However, this time around, your fellow
-100
poor quality control resulting in 20% of your Na channels (i.e. 100/500) being defective. These
defective channels lacked an inactivation gate (h) but their activation gate (m) was normal. Plot
the whole cell currents in response to the voltage waveform shown below. Assume your
recording solutions are the same as before.
IK
INa+IK
INa
Stable fixed point
Unstable fixed point
IK
INa+IK
INa
2E. (4 pts) Assuming 20% of your Na channels continue to be defective (lack h gate) as a
consequence of the manufacturing process, how would you redesign your system to allow it to
spike reliably.
One way would be to increase the number of K channels (in this case to 70 channels). Now
again, the K current is large enough to overwhelm the non-inactivating Na current resulting in a
single stable fixed point at -90 mV as shown below.
You could also decrease the number of Na channels to about 350, then again the system will
evolve to have only a single stable fixed point at -90 mV.