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Biochemistry: Experiment 1

Experiment 1: Dilution And Molarity


Dilution problems:
Molarity (M) = mol / L

i.

How would you prepare:

a. 10 mL of a 1:10 dilution of a 1 M NaCl solution and what would be the final


concentration of NaCl?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 1 M
V1 = x

M2 = 1:10 x 1 M

M2 = 1:10 x 1 M
V2 = 10 mL
M 2V 2
M1

V1 =

= 0. 1 M

(0. 1 M )(10 mL)


1M

= 1mL

Hence, 1mL of the stock solution must be added up with 9 mL of solvent.


b. 80 mL of a 1:20 dilution of a 1 M NaCl solution?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 1 M
V1 = x

M2 = 1:20 x 1 M
= 0. 05 M
mL

M2 = 1:20 x 1 M
V2 = 80 mL

V1 =

M 2V 2
M1
=

(0. 05 M )(80 mL)


1M

=4

Biochemistry: Experiment 1

Hence, 4 mL of the stock solution must be added up with 76 mL of solvent.


c. 50 mL of a 1:25 dilution of a 1 M NaCl solution?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 1 M
V1 = x

M2 = 1:25 x 1 M

M2 = 1:25 x 1 M
V2 = 50 mL

V1 =

M 2V 2
M1

= 0.04 M

(0. 04 M )(50 mL )
1M

=2

mL
Hence, 2 mL of the stock solution must be added up with 48 mL of solvent.
ii.

How would you prepare exactly 6 mL of a 1/20 dilution (assume the concentration of
your starting solution is 1)?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 1 M
V1 = x

M2 = 1:20 x 1 M
= 0.05 M

M2 = 1:20 x 1 M
V2 = 6 mL

V1 =

M 2V 2
M1
=

(0. 05 M )(6 mL)


1M

= 0.3

mL
Hence, 0.3 mL of the stock solution must be taken up and added up with 5.7 mL of
solvent.

Biochemistry: Experiment 1

iii.

You are provided with an antibody solution (Ab) that has a concentration of 600
microgram (g) / micriolitre (L). For lab, it is necessary to make the following
dilutions:
a. 10 L of 600 g/L Ab + 190 L of buffer to make a 1:20 dilution at _30__ g/L
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 600 g/L
V1 = 10 L

M2 =
=

M2 = x
V2 = 200 L

M 1V 1
V2

(600 g/ L)(10 uL)


200 L

= 30 g/L

V2 = 10 L + 190 L (stock solution + solvent)


b. 20 L of 1:20 Ab + 40 L of buffer to make 1:60 dilution at ___10___g/L
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1
= 1:20 x 600g/L
V1 = 20 uL

M2
= 1:60 dilution at
______g/L
V2 = 60 L

M1 = 1:20 x 600g/ L
= 30 g/L
g/L

M2 =
=

M 1V 1
V2
(30 g/ L)(20 L)
60 L

= 10

Biochemistry: Experiment 1
OR
M2 = 1:60 x 600g/ L = 10 g/L
V2 = 20 L + 40 L (stock solution + solvent)

c. 5 L of 1:60 Ab + 5 L of buffer to make a _

1
_ dilution at _ 5 g/L
120

Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1
= 1:60 x 600g/L

M2
=x

V1 = 5 L

V2 = 10 L

M1 = 1:60 x 600g/ L

M2 =

= 10 g/L

M 1V 1
V2
(1 0 g/ L)(5 L)
10 L

g/L
Dilution factor (M2)
=

5
600

1
120

V2 = 20 L + 40 L (stock solution + solvent)

d. 10 L of 1:60 Ab + 90 L of buffer to make a _

1
____ dilution at _1_g/L
600

Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.

=5

Biochemistry: Experiment 1
M1
= 1:60 x 600g/L

M2
=x

V1 = 10 L

V2 = 100 L

M1 = 1:60 x 600g/ L

M 1V 1
V2

M2 =

= 10 g/L

(1 0 g/ L)(10uL)
1 00 L

=1

g/L
Dilution factor (M2) =

1
600

V2 = 10 L + 90 L (stock solution + solvent)


e. 10 uL of 1:60 Ab + 40 L of buffer to make a _

1
300

_ dilution at _2_g/L

Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1
= 1:60 x 600g/L

M2
=x

V1 = 10L

V2 = 50 L

M1 = 1:60 x 600g/ L

M 1V 1
V2

M2 =

= 10 g/L

(1 0 g/ L)(10uL)
50 L

=2

g/L
Dilution factor (M2)
=

2
600

1
300

V2 = 10 L + 40 L (stock solution + solvent)


f. 10 uL of 1:60 Ab + 10 uL of buffer to make a _5 g/L_ dilution at _
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.

1
__g/L
120

Biochemistry: Experiment 1
M1 = 1:60 x600g/L

M2 = x

V1 = 10L

V2 = 20 L

M1 = 1:60 x 600g/ L

M2 =

= 10 g/L

M 1V 1
V2
(1 0 g/ L)(10uL)
20 L

=5

g/ L
Dilution factor (M2)
=

5
600

1
120

V2 = 10 L + 10 L (stock solution + solvent)

iv.

How much 2.0 M NaCl solution would you need to make 250 mL of 0.15 M NaCl
solution?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 0.15 M
V1 = 250 mL

V2 =

=
v.

M2 = 2.0 M
V2 = x

M 1V 1
M2

(0.15 M )(250 mL)


2.0 M

= 18.75 mL

What would be the concentration of a solution made by diluting 45.0 mL of 4.2 M


KOH to 250 mL?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 4.2 M
V1 = 45.0 mL

M2 = x
V2 = 250 mL

Biochemistry: Experiment 1

M2 =
=

vi.

M 1V 1
V2

(4.2 M )( 45.0 mL)


250 mL

= 0.756 mL

What would be the concentration of a solution made by adding 45.0 mL of 4.2 M


KOH to 250 mL?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 4.2 M
V1 = 45.0 mL

M2 =

vii.

M 1V 1
V2

M2 = x
V2 = 45.0 + 250 mL

(4.2 M )(45.0 mL)


295 mL

= 0.641 mL

How much 0.20 M glucose solution can be made from 50.0 mL of 0.50 M glucose
solution?

Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 0.50 M
V1 = 50.0 mL

V2 =
=
viii.

M2 = 0.20 M
V2 = x

M 1V 1
M2

(0.50 M )(50.0 mL)


0.20 M

= 125mL

What is the molarity of a solution that has 4.5 mol of solute dissolved in 300 mL of
solution?

Solution:
n = MV
Then, the given information is been put into the equation.

Biochemistry: Experiment 1
n = 4.5 mol
V = 0.3 L

M=
=

M= x

n
V
4.5 mol
0.3 L

= 15 M

ix.

What is the molarity of a solution of NaOH that has 0.491 g dissolved in 400 mL of
solution?
Solution:

n=

mass
molar mass

Then, the given information is been put into the equation.


n=x
Mass = 0.491 g

n=
M=

0.491 g
39.99

V = 400 mL
Molar mass (NaOH)
= 39.99

= 0.0123 mol

n
V
=

0.0123mol
400 mL

= 3.075 x 10 -5

Biochemistry: Experiment 1

x.

What is the molarity of a solution prepared by diluting 10.00ml of a 4.281 M solution


to 50.00 mL?

Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 4.281 M

M2 = x

V1

V2 = 50.00 mL

= 10.00ml

M2 =
=

M 1V 1
V2

(4.281 M )(10.00 ml)


50.00 mL

= 0.856mL