ABX Pentra 60 C Plus Analyzer Service Manual PDF

Pentra 60C+
Technical Manual
P/n: RAA021B
ABX DIAGNOSTICS
B.P. 7290
Rue du caduce
Parc Euromdecine
34184 MONTPELLIER Cedex 04 - FRANCE
Pentra 60 C+
Notice of Liability
The information in this manual is distributed on an as is basis, without warranty. While
every precaution has been taken in the preparation of the manual, ABX DIAGNOSTICS will not
assume any liability to any persons or entities with respect to loss or damage, caused or
alleged to be caused directly or indirectly by the instructions contained in this manual or by
the computer software and hardware products described herein.
Trademarks
Microsoft and Windows are registered trademarks of Microsoft corporation.
Other product names mentioned within this publication may be trademarks or registered
trademarks of other companies.
Graphics
All graphics including screens and printouts, photographs are for illustrations purposes
only and are not contractual.
Copyright 2004 by ABX DIAGNOSTICS

All rights reserved. No part of this book may be reproduced or transmitted in any form or
by any means, electronic, mechanical, photocopying, recording, or otherwise, without the
prior written permission of ABX DIAGNOSTICS.
II
Introduction
REVISIONS
INDEX
A
B
TECHNICAL NOTE SOFTWARE REVISION

RAH801A
RAN038A
V1.61
V2.2.9
SECTION
ALL
ALL
DATE
14/04/2001
15/02/2004
This document applies to the latest higher software version.

When a subsequent software version changes the information in this document, a new issue will be released.
III
Pentra 60 C+
1. WARNINGS AND PRECAUTIONS
NOTE
NOTE: Emphasizes the important information especially helpful to the operator before, during
or after a specific operational function.
IMPORTANT
IMPORTANT: Emphasizes an operating procedure that must be followed to avoid erroneous
results.
CAUTION
CAUTION: Emphasizes an operating procedure that must be followed to avoid possible
damage to the instrument or erroneous test results.
WARNING
WARNING: Flags a procedure that if not followed properly, can prove to be extremely
hazardous to either the operator or the environment or both.
1.1. Warnings
User manual must be enterely read and personnel trained by ABX DIAGNOSTICS before
attempting to operate instrument. The user always operates with full knowledge and
appreciation of instrument warnings, alarms and flags.
Always refer to labeling and ABX DIAGNOSTICS instructions in order to avoid to compromise
system integrity.
The ABX PENTRA 60 C+ responds to the Standards and directives named in the Declaration
of Conformity added at the beginning of this manual.
The reagents and accessoiries stipulated by ABX DIAGNOSTICS have been validated in
accordance with the European Directive for in-vitro medical devices (98/79/CE).
The use of any other reagents and accessoiries may place at risk the performance of the
instrument, engaging the Users reponsability. In this case, ABX DIAGNOSTICS takes no
responsability for the device nor for the results rendered.
Disposal gloves, eyes protection and lab coat must be worn by the operator. Local or
national regulations must be applied in all the operations.
Portable/mobile should not be used in proximity
All peripheral devices should be IEC compatible.
IV
Introduction
1.2. Limited garantee

The duration of guarantee is stipulated in the Sales conditions associated with the purchase
of this instrument. To validate the guarantee, ensure the following is adhered to:
1 - The system is operated under the instructions of this manual.
2 - Only software or hardware specified by ABX DIAGNOSTICS is installed on the instrument.
This software must be the original copyrighted version.
3 - Services and repairs are provided by an ABX DIAGNOSTICS authorized technician, using
only ABX DIAGNOSTICS approved spare parts.
4 - The electrical supply of the laboratory follows the national regulations.
5 - Specimens are collected and stored in normal conditions.
6 - Reagents used are those specified in this user manual.
7 - Proper tools are used when maintenance or troubleshooting operations are performed.
CAUTION
If this instrument has been supplied to you by anyone other than ABX
Diagnostics or an authorised representative, ABX Diagnostics cannot
guarantee this product in terms of specification, latest revision and latest
documentation. Further information may be obtained from your
authorised representative.
1.3. Safety precautions, electronic and moving parts

The following parts must not be handled or checked by the user:
- Electrical power supply,
- Electronic components.
Operator injury may occur from an electric shock. Electronic components can shock and
injure the user. Do not tamper with the instrument and do not remove any components
(covers, doors, panels and so on) unless otherwise instructed within this document.
Danger ! The battery may explode if it is not replaced correctly ! Replace only with the
same or equivalent type recommanded by the manufacturer. Dispose of used batteries
according to the manufacturers instructions.
Moving parts:
It is strictly forbidden to disable sensors as it may cause operator injuries. Protection covers
must not be opened during instrument operations.
Pentra 60 C+
1.4. Biological risks

Consider all Specimens, Reagents, Calibrators, Controls, etc that contain human blood
or serum as potentially infectious! Use established, good laboratory working practices when
handling specimens. Wear protective gear, Gloves, Lab coats, Safety glasses and/or Face
shields, and follow other bio-safety practices as specified in OSHA Blood borne Pathogens
Rule (29 CFR part 1910. 1030) or equivalent bio-safety procedures.
ABX DIAGNOSTICS uses disinfectant product for instrument decontamination and highly
recommends it to decontaminate your instrument.
1.5. Instrument cleaning

Instrument external cleaning
The external surfaces of the instrument must be decontaminated considering the biological
environment.
WARNING
Never spill liquid on the instrument.
Never use Disinfectant product* that contains alcohol.
Computer screen: Use a soft clot, slightly wet with disinfectant product*. Wipe gently the
screen and dry to remove any trace of moisture.
All contaminated surfaces (covers, counting assembly area...): Slightly wet a sponge with
disinfectant product* and wipe the dirty surfaces.
Stainless steel parts: Slightly wet a sponge with disinfectant product* and wipe the dirty
surfaces. Dry with a soft cloth.
*Products having the following microbiological properties:
Bactericidal
Fungicidal
Active on Aspergillus fumigatus
Active on Mycobacterium tuberculosis (B.K)
Antiviral (VIH, HBV and rotavirus)
Product Example validated by ABX DIAGNOSTiCS:
ANIOS detergent disinfectant ; WIPANIOS ; ref: 1316.424
NOTE
Please also refer to the W.H.O (World Health Organization) guidelines:
Laboratory Biosafety Manual, 2nd edition, for further information.
VI
Introduction
Instrument internal cleaning

Concentrated cleaning: Counting chambers and hydraulics parts are decontaminated by
using the Concentrated cleaning function as described in 2.1.2. Concentrated cleaning,
page 5-11.
Sampling probe
Sampling probe must be decontaminated as follows:
1- Prepare a solution of Sodium Hypochlorite to 100ml/l.
2- Fill a 5ml tube with this solution.
3- Run 5 analysis on bleach.
NOTE
Please also refer to the W.H.O (World Health Organization) guidelines:
Laboratory Biosafety Manual, 2nd edition, for further information.
VII
Pentra 60 C+
1.6. Graphics and symbols

Switch off position
Switch on position
Alternating current
Manufacturer
In Vitro Diagnostics medical device
This product conforms to the EEC Standards and Directives named in the
declaration of conformity
Caution, consult accompanying

documents
Biological risk
Reagent
Up
Fragile, handle with care
Keep dry
Do not stack
Temperature limitation
Batch code
Catalogue number
Use by
Consult Instructions for use
Calibrator
Control
Content
VIII
Introduction
2. WORKING CONDITIONS
2.1. Environment
The operation of the ABX PENTRA 60 C+ should be restricted to indoor location use only !
Operation of the instrument at altitudes of over 3000 Meters (9800 feet) is not recommended.
The instrument is designed for safety from voltages surges according to INSTALLATION
CATEGORY II and POLLUTION DEGREE 2(IEC EN 61010-1).
Please contact your local ABX DIAGNOSTICS representative for information regarding operation
locations, when it does not comply with the recommended specifications.
2.2. Location
The ABX PENTRA 60 C+ should be placed on a clean and levelled table or workbench. Please
note that the ABX PENTRA 60 C+, printer and reagents weigh approximately 40 kilograms (88
lbs).
Avoid exposure to sunlight.
Place your instrument where it is not exposed to water or vapor.
Place your instrument where it is free from vibration or shock.
Place your instrument where an independent power receptacle can be used.
Use a receptacle different from the one used by a device that easily generate noise such as
a centrifuge, etc...
Provide a space of at least 20 cm (8 inches) at the back of the instrument for arranging the
power cable and tubings.
The Power switch and Input voltage supply connection should always be accessible !
When positioning the system for operational use, leave the required amount of space for
easy accessibility to these items.
2.3. Grounding
Proper grounding is required when installing the system. Check the wall outlet ground
(Earth) for proper grounding to the facilities electrical ground. If you are unsure of the outlet
grounding, contact your facilities engineer to verify the proper outlet ground !
2.4. Humidity and temperature conditions

ABX PENTRA 60 C+ must operate between temperatures of 16 to 34C (61 to 93F). Maximum relative humidity should be 80% for temperatures up to 31C (88F) and decreasing
linearly to 50% relative humidity at 40C (104F). If the system is kept at a temperature of
10C (50F) or less, it must be allowed to sit at room temperature for 1 hour before it can be
used for operation.
IX
Pentra 60 C+
2.5. Electromagnetic environment check

The ABX PENTRA 60 C+ has been designed to produce less than the accepted level of
electromagnetic interference in order to operate in conformity with its destination, allowing
the correct operation of other instruments also in conformity with their destination.
In case of suspected electromagnetic noise, check that the instrument has not been placed
in the proximity of electromagnetic fields or short wave emissions, i. e. (Radar, X-rays, Scanners, Cell phones, etc...).
2.6. Environment protection

Disposal Used accessories and consumables must be collected by a laboratory specialized
in elimination and recycling of this kind of material according to the local legislation.
Disposal ABX PENTRA 60 C+ instrument:
It should be disposed of, in accordance with local legislation, and should be treated as being
contaminated with blood. The appropriate biological precautions should be taken.
2.7. Transport and storage conditions

Storage temperature: -20C +50C
Prior to the shipping of an instrument by transporter, whatever

the destination, an external decontamination of the instrument
must be carried out.
Introduction
INTRODUCTION
REVISIONS ........................................................................................................ III
1. WARNINGS AND PRECAUTIONS .................................................................. IV
1.1. Warnings ................................................................................................ IV
1.2. Limited garantee .................................................................................... V
1.3. Safety precautions, electronic and moving parts ................................... V
1.4. Biological risks ....................................................................................... VI
1.5. Instrument cleaning ............................................................................... VI
1.6. Graphics and symbols ......................................................................... VIII
2. WORKING CONDITIONS .............................................................................. IX
2.2. Location ................................................................................................ IX
2.3. Grounding ............................................................................................. IX
2.1. Environment .......................................................................................... IX
2.4. Humidity and temperature conditions ................................................... IX
2.5. Electromagnetic environment check ..................................................... X
2.6. Environment protection ......................................................................... X
2.7. Transport and storage conditions .......................................................... X
SPECIFICATIONS ............................................................................................. XII
HYDRAULIC & PNEUMATIC PRINCIPLES ........................................................XIII
ELECTRIC & ELECTRONIC PRINCIPLES ......................................................... XIV
ANALYSIS CYCLE TECHNOLOGY ................................................................... XV
SOFTWARE VERSIONS ................................................................................... XVI
OUTPUT FORMAT ......................................................................................... XVII
ALARMS & ERROR LIST ............................................................................... XVIII
MAINTENANCE .............................................................................................. XIX
EXPLODED VIEWS ..............................................................
XX
SPARE PART LIST .......................................................................................... XXI
XI
Pentra 60 C+
SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS .................................................................... 1-2
1.1. Parameters .......................................................................................... 1-2
1.1.1. CBC Mode .................................................................................................. 1-2
1.1.2. CBC + 5DIFF Mode ..................................................................................... 1-3
1.1.3. Units ..................................................................................................

1.2. Throughput Analyses ..........................................................................
1.3. Tube Identification ...............................................................................
1.4. Reagents .............................................................................................
1.5. Workstation Computer ........................................................................
1.6. Measurements and computation ........................................................
1-4
1-5
1-5
1-5
1-5
1-5
2. PHYSICAL SPECIFICATIONS ......................................................................

2.1. Power requirements ............................................................................
2.2. Operating temperature and humidity ..................................................
2.3. Dimensions and Weight ......................................................................
2.4. Minimum specimen volume ................................................................
2.5. Dilution ratios ......................................................................................
2.6. Hgb measurement .............................................................................
2.7. Counting aperture diameters ..............................................................
2.8. Reagent consumption (ml) .................................................................
1-6
1-6
1-6
1-6
1-6
1-7
1-7
1-7
1-8
3. SUMMARY OF PERFORMANCE DATA ....................................................... 1-9

3.1. Precision (Reproducibility)* .................................................................. 1-9
3.2. Precision (Repeatability) .................................................................... 1-10
Precision claims* ............................................................................................... 1-10
3.2. Linearity* ............................................................................................. 1-11

3.3. Carryover* .......................................................................................... 1-11
3.4. Normal ranges* ................................................................................. 1-12
3.5. Accuracy* .......................................................................................... 1-13
3.6. Leukocyte differential count ............................................................... 1-13
3.7. Sample stability study ........................................................................ 1-13
3.8. Flags giving an analysis default .......................................................... 1-14
4. REAGENTS SPECIFICATIONS .................................................................... 1-15
4.1. Reagent specifications ....................................................................... 1-15
4.2. Waste handling precautions .............................................................. 1-15
5. LIMITATIONS ............................................................................................. 1-16
5.1. Maintenance ...................................................................................... 1-16
5.2. Blood specimens ............................................................................... 1-16
5.3. Known interfering substances ........................................................... 1-17
XII
Introduction
HYDRAULIC & PNEUMATIC PRINCIPLES
1. HYDROPNEUMATIC CONNECTIONS .......................................................... 2-2
2. FUNCTION OF VALVES .............................................................................. 2-5
3. VALVES LOCATIONS .................................................................................. 2-6
4. PNEUMATIC DIAGRAM .............................................................................. 2-7
XIII
Pentra 60 C+
ELECTRIC & ELECTRONIC PRINCIPLES
1. MAIN BOARD TEST POINTS ....................................................................... 3-2
2. MAIN BOARD POTENTIOMETERS .............................................................. 3-4
3. PENTRA 60 C+ BOARDS ............................................................................ 3-5
3.1 Main board XAA 425 C ......................................................................... 3-5
3.2. Optical Preampli board XAA 423 B ..................................................... 3-6
3.3. Motor relay board XAA 427 B ............................................................. 3-7
3.4. Carriage board XAA 428 C ................................................................. 3-8
3.5. LED board XAA 429 A ......................................................................... 3-9
4. PENTRA 60 C+ CONNECTORS ................................................................ 3-10
4.1. Chamber Heating Connector XBA 386 A .......................................... 3-10
4.2. Reagent Heating Coil Connector XBA 387 A ..................................... 3-11
4.3. Horizontal Carriage Motor XBA 391 A ............................................... 3-12
4.4. Upper Fan XBA 393 A ....................................................................... 3-13
4.5. IR sensor XBA 394 AS ...................................................................... 3-14
4.6. IR sensor XBA 395 AS ...................................................................... 3-15
4.7. IR sensor XBA 396 AS ....................................................................... 3-16
4.8. IR sensor XBA 397 AS ....................................................................... 3-17
4.9. RBC / WBC coaxe XBA 398 B .......................................................... 3-18
4.10. LMNE flowcell coaxe XBA 399 AS ................................................... 3-19
4.11. Barcode reader cable XBA 402 AS .................................................. 3-20
4.12. Diluent level sensor XDA 605 AS ..................................................... 3-21
5. PENTRA 60 C+ SYNOPTIC ....................................................................... 3-22
XIV
Introduction
ANALYSIS CYCLE TECHNOLOGY
1. ANALYSIS CYCLE DESCRIPTION ................................................................ 2-2
2. MEASURING PRINCIPLES .......................................................................... 2-4
2.1. Multi Distribution Sampling System (MDSS) ......................................... 2-4
2.2. RBC / Plt detection principles ............................................................. 2-5
2.3. Hgb measurement ............................................................................. 2-6
2.4. Hct measurement ............................................................................... 2-7
2.5. RDW calculation .................................................................................. 2-7
2.6. MCV, MCH, MCHC calculation ............................................................ 2-7
2.7. MPV Measurement ............................................................................. 2-8
2.8. Pct calculation .................................................................................... 2-8
2.9. PDW calculation .................................................................................. 2-8
2.10. WBC and differential count ................................................................ 2-9
2.10.1. General principles .................................................................................... 2-9
2.10.2. BASO / WBC Count ................................................................................ 2-9
2.10.3. LMNE matrix .......................................................................................... 2-10
XV
Pentra 60 C+
SOFTWARE VERSIONS
1. SERVICE SOFTWARE OVERVIEW ............................................................... 5-2
2. SOFTWARE RELEASES .............................................................................. 5-3
2.1. Software releases table ....................................................................... 5-3
2.2. Software modifications ....................................................................... 5-3
XVI
Introduction
OUTPUT FORMAT
Refer to document ABX Diagnostics OUTPUT FORMAT RAA024
XVII
7-1
Pentra 60 C+
ALARMS & ERROR LIST
1. TRANSMISSION .......................................................................................... 7-2
2. DATABASE ................................................................................................. 7-2
3. WORKLIST ................................................................................................. 7-2
4. CALIBRATION ............................................................................................ 7-3
5. TECHNICAL OPERATIONS ......................................................................... 7-3
6. PRINTER .................................................................................................... 7-3
XVIII
Introduction
MAINTENANCE
1. MAINTENANCE .......................................................................................... 8-2
1.1. Introduction .......................................................................................... 8-2
1.2. Daily customer maintenance ............................................................... 8-2
1.3. Weekly customer maintenance ........................................................... 8-2
1.4. Maintenance table ............................................................................... 8-3
2. MAINTENANCE KITS .................................................................................. 8-4
2.1. 6 month maintenance kit ..................................................................... 8-4
2.2. Yearly maintenance kit ........................................................................ 8-4
2.3. Pistons maintenance kit ...................................................................... 8-5
2.4. Fitting kit ............................................................................................. 8-5
2.5. Screws kit ........................................................................................... 8-6
3. PROCEDURES ............................................................................................ 8-7
3.1. Required tools and product ................................................................. 8-7
3.2. Procedure chart table ......................................................................... 8-8
3.3. Procedure chart table in maintenance order ...................................... 8-9
XIX
Pentra 60 C+
EXPLODED VIEWS
XX
Introduction
SPARE PART LIST
SPARE PART LIST ......................................................................................... 11-2
XXI
Pentra 60 C+
SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS ................................................. 1-2
1.1. Parameters ....................................................................... 1-2
1.1.1. CBC Mode ................................................................ 1-2
1.1.2. CBC + 5DIFF Mode ................................................... 1-3
1.1.3. Units .............................................................................. 1-4
1.2. Throughput Analyses ....................................................... 1-5
1.3. Tube Identification ............................................................ 1-5
1.4. Reagents ......................................................................... 1-5
1.5. Workstation Computer ..................................................... 1-5
1.6. Measurements and computation ..................................... 1-5
2. PHYSICAL SPECIFICATIONS ................................................... 1-6
2.1. Power requirements ........................................................ 1-6
2.2. Operating temperature and humidity .............................. 1-6
2.3. Dimensions and Weight .................................................. 1-6
2.4. Minimum specimen volume ............................................ 1-6
2.5. Dilution ratios .................................................................. 1-7
2.6. Hgb measurement .......................................................... 1-7
2.7. Counting aperture diameters ........................................... 1-7
2.8. Reagent consumption (ml) .............................................. 1-8
3. SUMMARY OF PERFORMANCE DATA .................................... 1-9
3.1. Precision (Reproducibility)* .............................................. 1-9
3.2. Precision (Repeatability) ................................................. 1-10
Precision claims* .............................................................. 1-10
3.2. Linearity* ........................................................................ 1-11
3.3. Carryover* ...................................................................... 1-11
3.4. Normal ranges* .............................................................. 1-12
3.5. Accuracy* ...................................................................... 1-13
3.6. Leukocyte differential count ........................................... 1-13
3.7. Sample stability study ..................................................... 1-13
3.8. Flags giving an analysis default ...................................... 1-14
4. REAGENTS SPECIFICATIONS ................................................ 1-15
4.1. Reagent specifications .................................................... 1-15
4.2. Waste handling precautions ........................................... 1-15
5. LIMITATIONS .......................................................................... 1-16
5.1. Maintenance ................................................................... 1-16
5.2. Blood specimens ........................................................... 1-16
5.3. Known interfering substances ........................................ 1-17
Pentra 60 C+
1. TECHNICAL SPECIFICATIONS
The ABX PENTRA 60 C+ is a fully automated hematology analyzer used for in vitro diagnostic
testing of whole blood specimens. A control station (or workstation) is directly connected to
the instrument.
The ABX PENTRA 60 C+ is able to operate either in CBC mode (Cell Blood Count:12 parameters)
or in CBC + 5DIFF mode (5 population Differential count: 26 parameters).
1.1. Parameters
1.1.1. CBC Mode
WBC
White Blood Cell
RBC
Hgb
Hct
MCV
MCH
MCHC
RDW
Red Blood Cell

Hemoglobin concentration
Hematocrit
Mean Corpuscular Volume
Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin Concentration
Red Distribution Width
Plt
PDW *
MPV
Pct *
Platelets
Platelet Distribution Width
Mean Platelet Volume
Plateletcrit
* PDW and PCT have not been established as indications for this product,in the United States. The use of PCT and PDW should be restricted to research and
Investigational measurements only.
1-2
Specifications
1.1.2. CBC + 5DIFF Mode

WBC
LYM
MON
NEU
EOS
BAS
LIC *
ALY *
White Blood Cell

Lymphocytes % and #
Monocytes % and #
Neutrophils % and #
Eosinophils % and #
Basophils % and #
Large Immature Cell % and #
Atypical Lymphocyte % and #
RBC
Hgb
Hct
MCV
MCH
MCHC
RDW
Red Blood Cell

Hemoglobin concentration
Hematocrit
Mean Corpuscular Volume
Mean Corpuscular Hemoglobin
Mean Corpuscular Hemoglobin Concentration
Red Distribution Width
Plt
PDW *
MPV
Pct *
Platelets
Platelet Distribution Width
Mean Platelet Volume
Plateletcrit
* PCT, PDW, ALY and LIC have not been established as indications for this product,in
the United States. The use of PCT, PDW, ALY and LIC should be restricted to research
and Investigational measurements only.
1-3
Pentra 60 C+
1.1.3. Units
UNITS
STD
SI
mmol/l
JAPAN
STD
SI
mmol/l
JAPAN
STD
SI
mmol/l
JAPAN
STD
SI
mmol/l
JAPAN
WBC
103/mm3
109/l
109/l
102/mm3
RBC
106/mm3
1012/l
1012/l
104/mm3
HGB
g/dl
g/l
mmol/l
g/dl
HCT
%
l/l
l/l
%
PLT
103/mm3
109/l
109/l
104/mm3
MCV
m3
fl
fl
m3
MCH
pg
pg
fmol
pg
LYC
%
%
%
%
MCHC
g/dl
g/l
mmol/l
g/dl
LYC
#
#
#
#
RDW
%
%
%
%
MON
%
%
%
%
MPV
m3
fl
fl
m3
MON
#
#
#
#
PCT
%
10-2/l
10-2/l
%
NEU
%
%
%
%
PDW
%
%
%
%
NEU
#
#
#
#
EOS
%
%
%
%
EOS
#
#
#
#
BAS
%
%
%
%
BAS
#
#
#
#
ALY
%
%
%
%
ALY
#
#
#
#
LIC
%
%
%
%
LIC
#
#
#
#
To select the set of units refers to Chapter 4. Instrument configuration
1-4
Specifications
1.2. Throughput Analyses

CBC Mode (CBC): .............. 60/h
CBC + 5DIFF Mode (DIF): .. 60/h
1.3. Tube Identification

By means of the ABX WORKSTATION: Barcode labels or keyboard.
1.4. Reagents
ABX DILUENT (20 litres)
ABX CLEANER (1 litre, Integrated),
ABX EOSINOFIX (1litre, Integrated),
ABX BASOLYSE II (1 litre, Integrated),
ABX ALPHALYSE or BIOLYSE (0.4 litre, Integrated)
1.5. Workstation Computer

Processor frequency: .........
Memory capacity: ..............
Hard drive: ..........................
Floppy + CD-ROM
Keyboard: ...........................
Mouse: ...............................
RS232 port: ........................
Screen: ...............................
Operating System: .............
* Pentium II 350 Mhz (min.)

* 128 Mb (min.)
* 4 Gb (min.)
* 105 keys (Qwerty default or Option)
* Standard PS/2 compatible
* x2 (Analyser/Local network)
* 15 monitor (800x600 min.)
* Microsoft Windows NT 4.0 Service pack 4 GB or US
1.6. Measurements and computation

Impedance for WBC, Plt, RBC, BASO.
Photometry for Hgb.
Impedance and light scattering for LYM, MON, NEU, EOS, ALY and LIC.
Computation from stored data that was directly measured for Hct, MCV, MCH, MCHC,
RDW, MPV, Pct, PDW.
1-5
Pentra 60 C+
2. PHYSICAL SPECIFICATIONS
2.1. Power requirements

Power supplies: ................. * from 100Vac to 240Vac (+10%)
.................................. * 50 Hz to 60 Hz
Power Consumption: ......... * Analyzer and computer: 400 VA
.................................. * Printer (Depends on printer see Printers manual)
.................................. * EPSON EPL5900: 900 VA
2.2. Operating temperature and humidity

16 - 34C (61 - 93F) room temperature
Maximum relative humidity 80% for temperatures up to 31C (88F) decreasing linearly to
50% relative humidity at 40C (104F).
2.3. Dimensions and Weight

Analyzer dimensions: .........
..................................
..................................
Analyzer weight: .................
* Height: approximately 516 mm (20.3 in.)

* Width: approximately 444 mm (17.5 in.)
* Depth: approximately 481 mm (19 in.)
* approximately 35Kgs (77lbs)
Computer dimensions: ....... * See computers manual

Computer weight: .............. * See computers manual
2.4. Minimum specimen volume

CBC Mode (CBC): .............. * 30l
CBC + 5DIFF Mode (DIF): .. * 53l
1-6
Specifications
2.5. Dilution ratios

WBC/BASO ........................
LMNE .................................
RBC/Plt ..............................
Hgb ..................................
* 1/200
* 1/80
* 1/10 000
* 1/250
2.6. Hgb measurement

Hgb chamber, LED 555 nm.
Modified Drabkin method (cyanmethemoglobin)
Light source ....................... * Electroluminescent diode
Wavelength ........................ * 550nm + 10nm
2.7. Counting aperture diameters

WBC/BASO ........................ * 80 m
LMNE ................................. * 60 m
RBC/Plt .............................. * 50 m
1-7
Pentra 60 C+
2.8. Reagent consumption (ml)

CYCLE
REAGENT
DILUENT
CBC (CBC)
22.6
5DIFF (DIF)
28.5
Startup*
65.4
Shutdown
27
Diluent Prime
44.9
Cleaner Prime
X
Eosinofix Prime
X
Basolyse II Prime
X
Alphalyse Prime
2.1
All Reagents Prime 49
Autoclean
13.4
Autocontrol
25.4
BASOLYSE II
CLEANER
EOSINOFIX
ALPHALYSE
APPROXIMATE
DURATION
2.1
2.1
2.1
X
X
X
X
23.6
X
24
1
X
0.9
0.9
3.7
14
X
24.8
X
1.1
X
25.1
1
1.4
X
1
1
X
X
X
23.6
X
X
24
1
X
0.4
0.4
1.4
1
X
X
X
X
8.4
8.2
0.3
1
60
60
450
300
310
120
110
220
130
6
135
150
* for one background count only (maxi = 3)
1-8
Specifications
3. SUMMARY OF PERFORMANCE DATA
3.1. Precision (Reproducibility)*
The ABX PENTRA 60 C+ was initially calibrated with Minocal Calibrator (Lot No. CX322, Expiry
Date: 05-Aug-2002).
Three levels of ABX MINOTROL material (Lot No: JX108) were run in duplicate once daily
for a prolonged period (26th July 2002 to 30 August 2002) on all parameters.
The results were used to quantify within run precision, and Total Precision in accordance
with the NCCLS EP 5-A Guidelines.
PARAMETER
WBC
RBC
HGB
HCT
PLT
PARAMETER
WBC
RBC
HGB
HCT
PLT
ABX MINOTROL
CONTROL
WITHIN RUN
SD
SD OF RUN
SD OF
MEANS
MEANS
TOTAL
PRECISION (SD)
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
0.001
0.008
0.031
0.000
0.001
0.002
0.001
0.002
0.005
0.021
0.064
0.104
6.271
40.229
154.146
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
0.001
0.013
0.045
0.000
0.001
0.003
0.001
0.007
0.012
0.025
0.122
0.181
20.646
72.103
381.388
ABX MINOTROL
CONTROL
WITHIN RUN
CV%
CV% OF RUN
CV% OF DAILY
MEANS
MEANS
TOTAL
PRECISION (CV%)
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
JX108 Low
JX108 Normal
JX108 High
1.8
0.9
0.9
0.8
0.6
0.7
0.4
0.3
0.4
0.9
0.7
0.7
3.1
2.6
2.5
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
1.93
1.12
1.05
0.86
0.79
0.88
0.57
0.59
0.60
0.99
0.97
0.89
5.69
3.46
4.01
*Source: 510K submission K030144

1-9
DAILY
Pentra 60 C+
Precision claims*
PARAMETER
% CV
NOMINAL VALUES
WBC
RBC
HGB
HCT
PLT
< 2.0%
< 2.0%
< 1.0%
< 2.0%
< 5.0%
10.0 x 103/mL
4.67 x 106/mL
13.6 g/dl
36.0 %
243 x 103/mL
3.2. Precision (Repeatability)

Based on 10 consecutive samples of the same fresh whole blood sample without alarm:
PARAMETERS
WBC
RBC
Plt
Hgb
Hct
%CV
<2%
<2%
<5%
<1%
<2%
1-10
TEST
LEVEL
at 10x103/L
at 5x106/L
at 300x103/L
at 15 g/dL
at 45%
Specifications
3.2. Linearity*
Linearity range: The Manufacturers tested linearity zone of the instrument using linearity
kits and/or human blood.
Linearity limits: Maximum and minimum values within instrument returns no dilution alarm.
Visible range: Range values given by the instrument. These values (above linearity limits)
are given as an indication. They are given associated with a D flag. This Visible range is
outside Manufacturers range.
Linearity kits
Linearity was tested using available Low Range and Full Range Linearity Test kits. The
Test kits were analyzed and data was computed according to the Manufacturers instructions.
Human Blood
Linearity was also obtained on human blood, using a minimum of 5 dilution points. The
re-sults of this study are as followed:
PARAMETER
WBC (103/L)
RBC (106/L)
HGB (g/dL)
HCT (%)
PLT (103/L)
LINEARITY
LINEARITY
VISIBLE
RANGE
LIMITS
RANGE
DIFFERENCE
0.40 130.80
0.23 9.76
0.00 31.06
1.80 88.90
3.30 2007
0 120
0 8.00
0 24
0 67
0 1900
120 150
8.00 18.00
24 30
67 80
1900 2800
0.3 7%
0.07 2%
0.3 2%
2.0 3%
10 10%
0 2800
2800 3200
10 10%
(Hgb>2g/dL,RBC>0.5x106/L)
PLT (103/L)
7.00 - 2895
6
(Hgb<2g/dL,RBC<0.5x10 /L)
3.3. Carryover*
The ABX PENTRA 60 C+ carry-over effects were evaluated by assaying a sample with high
cell concentrations three consecutive times (i1-3), followed immediately by testing a diluted
sample consecutively 3 times (j1-3).
(j1-j3)
Carryover =
X 100
(i3-j3)
Carry-over % is then:
Mean low levels

Mean high levels
Carry over %
WBC
RBC
HGB
PLT
1.06
58.81
-0.260
1.58
6.37
0.000
5.28
22.03
-0.179
31.33
1106.67
-0.186
This is the method as described in Guidelines for the Evaluation of blood cell analyzers
including those used for differential leukocyte and reticulocyte counting and cell marker
applications. ISLH, 14 January, 1994.
1-11
Pentra 60 C+
Carry-over Conclusion:
Results provided are extremely satisfactory. In order to provide for eventual possibilities
within the laboratory environment the following claims shall be made :
Claims
WBC
RBC
HGB
PLT
< 2.0%
< 2.0%
< 2.0%
< 2.0%
3.4. Normal ranges*

PARAMETERS
WBC (103/L)
RBC (106/L)
HGB (g/dL)
HCT (%)
MCV (m3)
MCH (pg)
MCHC (g/dL)
RDW (%)
PLT (103/L)
MPV (m3)
PCT (%)
PDW (%)
NEU (%)
LYM (%)
MON (%)
EOS (%)
BAS (%)
MALE
FEMALE
4 - 10
4.50 - 6.50
13.0 - 17.0
40.0 - 54.0
80 - 100
27.0 - 32.0
32.0 - 36.0
11.0 - 16.0
150 - 500
6 - 11
0.15 - 0.50
11 - 18
50 - 80
25 - 50
2 - 10
0-5
0-2
4 - 10
3.80 - 5.80
11.5 - 16.0
37.0 - 47.0
80 - 100
27.0 - 32.0
32.0 - 36.0
11.0 - 16.0
150 - 500
6 - 11
0.15 - 0.50
11 - 18
50 - 80
25 - 50
2 - 10
0-5
0-2
IMPORTANT
Expected values will vary with sample population and/or geographical
location. It is highly recommended that each Laboratory establish its
own Normal ranges based upon the local population!
*Bibliography:
AIDE MEMOIRE DHEMATOLOGIE
Prof : C.SULTAN / M. GOUAULT- HELMANN / M. IMBERT
Service Central dHmatologie de lHopital Henri Mondor
Facult de mdecine de Crteil (Paris XII)
1-12
Specifications
3.5. Accuracy*
The data shows good correlation between results achieved on the ABX PENTRA 60 C+
versus the reference system, which can be resumed as follows:
PARAMETER
R(COMPARISON OF
WBC
PLT
RBC
HGB
HCT
LYM
NEU
MON
EOS
BAS
0.997
0.998
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
MEANS)
3.6. Leukocyte differential count

Not available at the time of publication.
3.7. Sample stability study

Not available at the time of publication.
1-13
ACCURACY CLAIMS
>0.95
>0.95
N/A
N/A
N/A
N/A
N/A
N/A
N/A
N/A
Pentra 60 C+
3.8. Flags giving an analysis default
Results displayed
PARAMETER
WBC(x103/mm3)
RBC(x106 /mm3)
PLT(x103/mm3)
HGB>2g/dL
HGB<2g/dL
HGB(g/dL)
HCT(%)
LINEARITY
VISIBLE
>VISIBLE
LIMITS
RANGE
RANGE
result
result
result
result
result
result
result+ D
result+ D
result+ D
result+ D
result+ D
result+ D
DIL
DIL
DIL
DIL
DIL
DIL
Results transmitted and printed out

PARAMETER
WBC(x103/mm3)
RBC(x106 /mm3)
PLT(x103/mm3)
HGB>2g/dL
HGB<2g/dL
HGB(g/dL)
HCT(%)
LINEARITY
VISIBLE
>VISIBLE
LIMITS
RANGE
RANGE
result
result
result
result
result
result
result+D
result+D
result+D
result+D
result+ D
result+ D
--.-- + D
--.-- + D
--.-- + D
--.-- + D
--.-- + D
--.-- + D
NOTE
Use the instrument diluent to dilute the sample if a ---- D flag occurs
on WBC or Hct.
1-14
Specifications
4. REAGENTS SPECIFICATIONS
4.1. Reagent specifications

In order for the instrument to operate correctly, high-quality reagents must be used. ABX
DIAGNOSTICS provides all the necessary reagents.
NOTE
The ABX Diagnostics reagents specified for this instrument may have
followed one or both of the approval methods below:
1 - have been registered by the A.F.S.S.A.P.S. Agence Franaise de
Scurit Sanitaire des Produits de Sant according to the procedure
relative to laboratory reagents used for biological analyses.
2 - or approved in accordance with the European Directive 98/79/CE
(Annex III) for in-vitro medical devices.
Please refer to the packaging of each reagent concerned to establish
the approval method.
Refer to Chapter Annex of this manual for all reagent speifications.
4.2. Waste handling precautions
WARNING
When disposing of waste, protective clothing must be worn (lab coat,
gloves, eye protection, etc). Follow your local and /or national
guidelines for biohazard waste disposal.
If required, waste can be neutralized before being discarded. Follow your laboratorys
protocol when neutralizing and disposing of waste.
Dispose of the waste container according to the local or national regulatory requirements.
1-15
Pentra 60 C+
5. LIMITATIONS
WARNING
Whilst every effort is taken by ABX Diagnostics to investigate and
indicate all known interferences, it is by no means possible to guarantee
that all interferences have been identified. At all times, results should
be validated and communicated only once all information relating to
the patient has been assessed and taken into account.
5.1. Maintenance
In Chapter 5. Maintenance & Troubleshooting, specific maintenance procedures are listed.
The maintenance procedures identified are mandatory for proper use and operation of the
ABX PENTRA 60 C+. Failure to execute any of these recommended procedures may result in
poor reliability of the system.
IMPORTANT
Failure to execute any of these recommended procedures may result
in poor reliability of the system.
5.2. Blood specimens

Verification of any abnormal test result (including flagged results or results outside of the
normal range) should be performed using reference methods or other standard laboratory
procedures for conclusive verification of the results. The sections below list known limitations of automated blood cell counters which use the principles of impedance and light
absorbance as principles of measurement.
1-16
Specifications
5.3. Known interfering substances

White Blood Cells (Leukocytes):
WBC results that exceed the linearity limits of the system will require dilution of the blood
sample (Leukemia sample followed by a leukopenia).
Re-assaying the diluted sample will help to obtain the correct assay value.
Unlysed Red Cells - In some rare instances, the erythrocytes in the blood sample may not
be completely lysed. These non-lysed red blood cells may be detected on the WBC histogram
with an L1 alarm or as an elevated baseline on the side (leading edge) of the lymphocytes
population. Non-lysed erythrocytes will cause a falsely elevated WBC count.
Multiple myeloma - The precipitation of proteins in multiple myeloma patients may give
high WBC counts.
Leukemia - A very low WBC count may result from this disease because of possible increased
fragility of the leukocytes leading to destruction of some of these cells during counting.
These white cell fragments will also interfere with the white cell differential parameters.
These white cell fragments will also interfere with the white cell partial differential parameters:
LYM% + #, MON% + #, GRAN% + #. A suspiciously low WBC count may also be seen in
patients with lymphocytic leukemias due to the presence of abnormally small lymphocytes
which may not be counted by the instrument.
Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of the
leukocytes which may cause low WBC counts.
Cryoglobulins - Increased levels of cryoglobulin that may be associated with myeloma,
carcinoma, leukemia, macroglobulinemia, lymphoproliferative disorders, metastic tumors,
autoimmune disorders, infections, aneurism, pregnancy, thromboembolic phenomena,
diabetes, etc, which can increase the WBC, RBC or PLT counts and the HGB concentration.
The specimen must be warmed up to 37C (99F) in a bain marie for 30 minutes and analyzed
again immediately after (analyzer or manual method).
Macrothrombocytes - in excessive numbers may affect and increase Leukocyte numeration.
RBC: Red Blood Cells (Erythrocytes)

The red blood cell dilution contains all the formed elements in the blood: erythrocytes,
leukocytes and platelets. During erythrocytes counting (red blood cells), platelets are not
counted as their size falls below the minimum threshold.
Agglutinated erythrocytes - May cause a low incorrect RBC count. Blood samples
containing the agglutinated red blood cells may be suspected by elevated MCH and MCHC
values and shown by examination of the stained blood film.
Cold agglutinins - IgM immunoglobulins which are high in cold agglutinin disease may
cause lower RBC and PLT counts and increase MCV.
1-17
Pentra 60 C+
Hgb (Hemoglobin):
Turbidity of the blood sample - Any number of physiological and/or therapeutic factors
may produce high incorrect HGB results. To obtain accurate hemoglobin results when
increased turbidity of the blood sample occurs, determine the cause of the turbidity and
follow the appropriate method below:
High WBC: An extremely high WBC will cause excessive light scatter. In these cases use
reference (manual) methods.The diluted sample should be centrifuged, and the supernatant
fluid measured with a spectrophotometer.
High lipid concentration: A high concentration of lipids in the blood sample will give the
plasma a milky appearance. This condition can occur with hyperlipidemia, hyperproteinemia
(as in gammapathies) and hyperbilirubinemia. Accurate hemoglobin determinations can be
achieved by using reference (manual) methods and a plasma blank.
Increased turbidity may also be seen in cases where the red blood cells are resistant to
lysing. This condition will cause an incorrect high HGB result, but may be detected by
observing the abnormal MCH, MCHC values, and the increased baseline on the leading
edge of the WBC histogram. Erroneous hemoglobin results will cause the results of the
MCH and MCHC to be incorrect as well.
Fetal bloods - The mixing of fetal and maternal bloods may produce a high inaccurate HGB
value.
Hct (Hematocrit)
Red blood cells agglutination - May produce an inaccurate HCT and MCV values. Red
blood cell agglutination may be detected by observing abnormal MCH and MCHC values,
as well as by examination of the stained blood film In such cases, manual methods may be
required to obtain an accurate HCT value.
MCV (Mean Corpuscular Volume)

Red blood cell agglutination - May produce an inaccurate MCV value. Red blood cell
agglutination may be detected by observing abnormal MCH and MCHC values, as well as
by examination of the stained blood film. In such cases, manual methods may be required to
obtain an accurate MCV value.
Excessive numbers of large platelets and/or the presence of an excessively high WBC
count may interfere with the accurate determination of the MCV value. In such cases, careful
examination of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin)

The MCH is determined according to HGB value and the RBC count. The limitations listed
for the HGB and RBC will have an effect on the MCH and may cause inaccurate values.
MCHC (Mean Corpuscular Hemoglobin Concentration)

The MCHC is determined according to the HGB and HCT values. The limitations listed for
the HGB and HCT will have an effect on the MCHC and may cause inaccurate values.
1-18
Specifications
RDW (Red blood cell Distribution Width)

The red blood cell distribution width is determined according to the RBC count.
Nutritional deficiency or blood transfusion - May cause high RDW results due to iron
and/or cobalamin and /or folate deficiency.
Plt (Platelets)
Very small erythrocytes (microcytes), erythrocyte fragments (schizocytes) and WBC fragments may interfere with the proper counting of platelets and cause elevated PLT counts.
Agglutinated erythrocytes - May trap platelets, causing an erroneously low platelet count.
The presence of agglutinated erythrocytes may be detected by observation of abnormal
MCH and MCHC values and by careful examination of the stained blood film.
Giant platelets in excessive numbers - may cause a low inaccurate platelet count as
these large platelets may exceed the upper threshold for the platelet parameter and are not
counted.
Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of
these cells which may cause low PLT counts. Reference (manual) methods may be necessary
to obtain an accurate platelet count.
Hemolysis - Hemolysed specimens contain red cell stroma which may increase platelet
counts.
A.C.D. blood - Blood anticoagulated with acid-citrate-dextrose may contain clumped platelet
which could decrease the platelet count.
Elevated triglycerides and/or cholesterol: may interfere with correct platelet counting.
Platelet agglutination - Clumped platelets may cause a decreased platelet count and/or a
high WBC count. The specimen should be recollected in sodium citrate anticoagulant to
ensure the anticoagulated character depending on agglutination and reanalyzed only for the
platelet count. The final PLT result must be corrected for the sodium citrate dilution effect.
However, these platelet clumps do trigger flags L1, LL and LL1.
MPV (Mean Platelet Volume)

Giant platelets that exceed the upper threshold of the Platelet parameter may not be counted
as platelets. Consequently, these larger platelets will not be included in the instruments
calculation of Mean Platelet Volume.
Very small erythrocytes (microcytes), erythrocytic fragments (Schizocytes) and white blood
cell fragments may interfere with the proper counting and sizing of Platelets.
Agglutinated erythrocytes - May trap Platelets, causing an incorrect MPV result. The
presence of agglutinated erythrocytes may be detected by observation of abnormal MCH
and MCHC values and by careful examination of the stained blood film.
Chemotherapy - May also affect the sizing of PLTs.
IMPORTANT
Blood samples collected in EDTA will not maintain a stable Mean
Platelet Volume. Platelets collected in EDTA swell depending on the
time post-collection and storage temperature.
1-19
Pentra 60 C+
LYM# (Lymphocyte count absolute value), LYM% (Lymphocyte percentage)
The Lymphocyte count is derived from the WBC count. The presence of erythroblasts, certain parasites and erythrocytes that are resistant to lysis may interfere with an accurate LYM
count. Limitations listed for the WBC count pertain to the LYM # and % counts as well.
MON# (mononuclear cell count absolute), MON% (Mononuclear percentage)

The mononuclear cell count absolute is derived from the WBC count. The presence of large
lymphocytes, atypical lymphocytes, blasts and an excessive number of basophils may
interfere with an accurate monocyte count. Limitations listed for the WBC count pertain to
the MON # and % counts as well.
NEU# (neutrophil count absolute), NEU% (Neutrophil percentage)

The neutrophils cell count is derived from the WBC cell count. The excessive presence of
eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may
interfere with an accurate neutrophils count.
EOS# (Eosinophil cell count absolute), EOS% (Eosinophil percentage)

The eosinophil cell count is derived from the WBC cell count. The presence of abnormal
granules (degranulated areas, toxic granules...) may interfere with the eosinophil counting.
BAS# (Basophil cell count absolute), BAS% (Basophil percentage)

The Basophil cell count is derived from the WBC cell count.
1-20
Pentra 60 C+
HYDRAULIC&PNEUMATIC PRINCIPLES
1. HYDROPNEUMATIC CONNECTIONS ..................................... 2-2
2. FUNCTION OF VALVES .......................................................... 2-5
3. VALVES LOCATIONS .............................................................. 2-6
4. PNEUMATIC DIAGRAM .......................................................... 2-7
Pentra 60 C+
1. HYDROPNEUMATIC CONNECTIONS
Read this table as follow: LV3_2S means connection to port 2 of valve 3 with a Sleeving.
FROM
ABX CLEANER
LENGTH
DIAM.
TO
Abx Cleaner Bottle

LV7_3
LV7_2
2.05
2.05
1.52
400
120
230
LV7_1
Diluter Syringe_2
LV12_3
LV12_1
LV12_2
1.52
1.52
350
280
Baso/WBC chamber_1
T3_3
Abx Eosinofix Bottle

LV8_3
2.05
2.05
400
120
LV8_1
Diluter Syringe_3
LV8_2
Reagent Heater_4S
1.52
1.52
500
120
Reagent Heater_3S
LMNE chamber_3
Abx Basolyse II Bottle

LV11_3
LV11_2
2.05
2.05
1.52
400
120
500
LV11_1
Diluter Syringe_5
Reagent Heater_10S
Reagent Heater_9S
Reagent Heater_12S
1.52
1.52
60
120
Reagent Heater_11S
BASO/WBC chamber_2
Abx Alphalyse Bottle

LV6_3
1.52
1.52
350
130
LV6_1
Diluter Syringe_1
LV6_2
1.52
480
DIL1/HGB chamber_2
1.02
205
Sampling Syringe_1
LMNE chamber_4
M1_1 photocell
LV4_2
1.30
1.30
1.02
20
320
130
M1_2 photocell
LV4_1
T2_2
T2_3
Injector Syringe_3
LV5_2
1.02
1.02
2.05
240
95
420
Injector Syringe_5
LV5_1
T6_2
T2_1S
LMNE flowcell_Output
1.85
0.19
1.52
11
4
20
LMNE flowcell_6
LMNE flowcell_6
E1_1 Anode fitting
E1_2 Anode fitting

Isolator_2
E2_2 Ground fitting
1.52
1.52
1.52
70
80
335
Isolator_1
E2_1 Ground fitting
LV24_2
LV24_1
T4_1
Reagent Heater_6S
1.52
1.52
1.52
120
160
120
T4_2
Reagent Heater_5S
LMNE chamber_2
ABX EOSINOFIX
ABX BASOLYSE II
ABX ALPHALYSE
SAMPLING
Probe_1
LMNE COUNTING
2-2
Hydraulic &
Pneumatic Principles
FROM
DILUENT
LENGTH
DIAM.
TO
Diluent Input
LV25_1S
2.54
2.54
2.05
170
140
65
LV25_2S
Diluent Tanker_4S
Diluent Tanker_1
Diluent Tanker_1
LV3_3S
LV3_2S
2.05
2.05
1.52
350
120
50
LV3_1
Injector Syringe_2
LV2_3S
LV2_1S
LV1_1S
Isolator_2S
1.52
1.52
1.52
50
200
100
LV1_3S
Isolator_1S
T1_1
T1_2
T1_2
T1_3
T1_3
1.14
XBA403
1.14
XBA403
35
LMNE flowcell_4
LMNE flowcell_4
LMNE flowcell_2
LMNE flowcell_2
LV1_2
LV2_2
Injector Syringe_1
1.52
1.02
1.02
250
175
205
T4_3
Injector Syringe_4
LMNE flowcell_5
LMNE flowcell_7
Diluent Tanker_3
1.02
2.05
2.05
10
65
480
(Cap)
Diluent Tanker_3
LV9_1
LV9_3
LV9_2
LV10_1
2.05
1.52
1.52
120
50
15
Diluter Syringe_4
LV10_3
T3_2
T3_1
LV18_1
LV19_1
1.52
1.52
1.52
640
50
190
LV18_3
LV19_3
T12_1
T12_3
T12_2
Isolator_3
1.52
1.30
1.52
75
15
215
Probe Rinsing Body_1

Isolator_1
LV32_1
LV32_2
LV19_2
LV18_2
1.52
1.52
1.02
780
300
115
T5_2
Probe Rinsing Body_2
Sampling Syringe_2
LV10_2
LV21_1
LV22_1
1.52
1.52
1.52
380
50
200
LV21_3
LV22_3
Reagent Heater_7S
Reagent Heater_8S
LV22_2
Reagent Heater_2S
1.52
1.52
1.52
120
260
120
RBC chamber_1
Reagent Heater_1S
DIL1/HGB chamber_1
LV21_2
1.52
220
Baso/WBC chamber_4
35
2-3
Pentra 60 C+
FROM
LENGTH
WBC/RBC COUNTING
DIAM.
TO
BASO/WBC chamber_3
1.52
200
LV23_3
LV23_1
RBC chamber_2
LV14_1
1.52
1.52
1.52
220
430
70
RBC chamber_3
LV14_2
Counting Syringe_2
LV23_2
1.52
310
Counting Syringe_3
Rinse chamber_3
Filter_2
LV27_1
2.05
2.05
2.05
30
30
30
Filter_1
LV27_2
T7_3
DIL1/HGB chamber_3
LV28_1
T7_1
2.05
2.05
2.05
70
20
20
LV28_2
T7_2
T8_3
LMNE chamber_5
LV29_1
T8_1
RBC chamber_4
2.05
2.05
2.05
2.05
70
20
20
60
LV29_2
T8_2
T9_3
LV30_2
LV30_1
T9_1
BASO/WBC chamber_5
2.05
2.05
2.05
20
20
55
T9_2
T10_2
LV31_2
LV31_1
T10_3
T6_1
2.05
2.05
2.05
20
30
20
T10_1
T6_3
T5_3
T5_1
M2_2 photocell
Isolator_2
2.05
2.05
2.05
50
15
280
M2_1 photocell
Isolator_1
LV26_1
LV26_3S
LV26_2S
Counting Syringe_5
2.54
2.54
2.05
150
130
125
Draining Syringe_2S
T11_3
LV16_1
LV16_2S
T11_1
2.54
2.54
340
20
T11_2
Waste Output
Atmosphere
LV13_1
2.05
1.52
140
70
LV13_2
Counting Syringe_1
Atmosphere
LV15_3
LV15_2
2.05
1.52
1.52
100
400
70
LV15_1
Diluent Tanker_2
Counting Syringe_4
Atmosphere
LV17_1
Rinse_2 chamber (Atmo.)
2.05
1.30
2.05
70
185
300
LV17_2
Isolator_2
LV20_2
LV20_1
1.52
90
Draining Syringe_1
DRAININGS
AIR
2-4
Hydraulic &
2. FUNCTION OF VALVES
VALVE #
VALVE SUBJECT
VALVE FUNCTIONS
1
2
3
DIL LMNE
DIL LMNE
DIL LMNE
Directing optical chamber / LMNE chamber

Directing Internal / External sleeving
Control Entry / Exit syringe
4
5
6
LMNE
LMNE
LYSE
Transfer control LMNE tray /Injection syringe

Transfer control LMNE tray /Injection syringe
7
8
9
CLEANER
EOSINOFIX
DIL

10
11
12
DIL
BASOLYSE II
CLEANER
Directing Circuit chamber / Needle circuit

Directing WBC/BASO chamber / Needle circuit
13
14
15
16
COUNTING SYRINGE
COUNTING SYRINGE
TANK
COUNTING SYRINGE
Set in the atmosphere

RBC/PLT count
Draining
17
18
19
NEEDLE
NEEDLE
NEEDLE
Flushing out of rinsing unit

Commutation Diluent interior/exterior Needle
Probe cleaning
20
21
22
DRAINING SYRINGE
DIL
DIL

Directing head for WBC count / Valve 22
Directing diluent chamber dil.1
23
24
25
COUNTING SYRINGE
LMNE
DILUENT TANK
WBC/RBC selection count

Exit from the optical chamber
Entry of the diluent
26
27
28
FLUSHING SYRINGE
CHAMBER GROUP
CHAMBER GROUP
Flushing of the syringe

Drain rinse chamber
Drain first dilution chamber
29
30
31
CHAMBER GROUP
CHAMBER GROUP
CHAMBER GROUP
Drain LMNE chamber

Drain RBC/PLT chamber
Drain WBC/BASO chamber
32
NEEDLE
Probe cleaning
2-5
Pentra 60 C+
3. VALVES LOCATIONS
Valve blocks are located close to the elements concerned. There are 5 different blocks:
Valves 1 to 11:
On the left side,
Horizontal block,
On the top of the reagent and injection syringes.
Valves 12 to 16:
On the left side,
Vertical block,
Beside the counting syringe.
Valves 17 to 19 & 32:

On the right side,
Horizontal block,
On ampling carriage.
Valves 20 to 26:
On the right side,
Vertical block,
Beside the draining syringe.
Valves 27 to 31:
On the right side,
Horizontal block,
On top of the blending and count chambers.
2-6
Hydraulic &
4. PNEUMATIC DIAGRAM
See Pneumatic diagram on next page.
2-7
Pentra 60 C+
ELECTRIC&ELECTRONIC PRINCIPLES
1. MAIN BOARD TEST POINTS ................................................... 3-2
2. MAIN BOARD POTENTIOMETERS ......................................... 3-4
3. PENTRA 60 C+ BOARDS ........................................................
3.1 Main board XAA 425 C ....................................................
3.2. Optical Preampli board XAA 423 B .................................
3.3. Motor relay board XAA 427 B .........................................
3.4. Carriage board XAA 428 C .............................................
3.5. LED board XAA 429 A ....................................................
3-5
3-5
3-6
3-7
3-8
3-9
4. PENTRA 60 C+ CONNECTORS ............................................. 3-10

4.1. Chamber Heating Connector XBA 386 A ....................... 3-10
4.2. Reagent Heating Coil Connector XBA 387 A .................. 3-11
4.3. Horizontal Carriage Motor XBA 391 A ........................... 3-12
4.4. Upper Fan XBA 393 A ................................................... 3-13
4.5. IR sensor XBA 394 AS ................................................... 3-14
4.6. IR sensor XBA 395 AS ................................................... 3-15
4.7. IR sensor XBA 396 AS ................................................... 3-16
4.8. IR sensor XBA 397 AS ................................................... 3-17
4.9. RBC / WBC coaxe XBA 398 B ...................................... 3-18
4.10. LMNE flowcell coaxe XBA 399 AS ............................... 3-19
4.11. Barcode reader cable XBA 402 AS .............................. 3-20
4.12. Diluent level sensor XDA 605 AS .................................. 3-21
5. PENTRA 60 C+ SYNOPTIC ................................................... 3-22
Pentra 60 C+
1. MAIN BOARD TEST POINTS
TEST POINT
DESIGNATION
POTEN-
TARGET
TOLERANCE
REMARK
TIOMETER
TP1 HB Blank adjustment

TP2 PLT Threshold adjustment
TP3 LMNE CIS Threshold adjustment
R248
R159
R160
4.7V
300mV
650mV
+/- 0.05V
+/- 5mV
+/- 5mV
TP4 LMNE OD Threshold adjustment

TP5 Draining syringe motor
TP6 Counting syringe motor
R161
R149
R150
350mV
4.8V
4.8V
+/- 5mV
+/- 0.05V
+/- 0.05V
TP7 Dilutor syringe motor

TP8 Injection syringe motor
TP9
UNUSED
R151
R152
4V
3V
+/- 0.05V
+/- 0.05V
TP10 Horizontal carriage motor

TP11 Sample syringe motor
TP12 Probe carriage motor
R154
R155
R156
3V
2V
4.5V
+/- 0.05V
+/- 0.05V
+/- 0.05V
TP13 RBC Threshold adjustment

TP14 BASO Threshold adjustment
TP15 BASO Comparator
R158
R157
300mV
300mV
+/- 5mV
+/- 5mV
TP19 LMNE OD Comparator

TP20 RBC line height adjsutment
TP21 BASO gain adjustment
R133
R134
XX V
XX V
+/- 50mV
+/- 50mV
Factory Adjusted
Factory Adjusted
TP22 PLT line height adjustment

TP23 LMNE Resistive gain adjustment
TP24 LMNE Optical gain adjustment
R135
R136
R148
XX V
XX V
XX V
+/- 50mV
+/- 50mV
+/- 50mV
Factory Adjusted
Factory Adjusted
Factory Adjusted
TP25 RBC line width adjustment

TP26 RBC line reject adjustment
TP27 RBC line pulse adjustment
R142
10s
+/- 0.5s
Factory Adjusted
TP28 BASO line width adjustment

TP29 BASO line reject adjustment
TP30 BASO line pulse adjustment
R139
10s
+/- 0.5s
Factory Adjusted
TP31 PLT line width adjustment

TP32 PLT line reject adjustment
TP33 PLT line pulse adjustment
R144
10s
+/- 0.5s
Factory Adjusted
TP34 LMNE Resistive line width adjustment

TP35 LMNE Resistive line reject adjustment
TP36 LMNE Resistive line pulse adjustment
R145
15s
+/- 0.5s
Factory Adjusted
TP37 LMNE Optical line width adjustment

TP38 LMNE Optical line reject adjustment
TP39 LMNE Optical line pulse adjustment
R147
5s
+/- 0.5s
Factory Adjusted
TP16 RBC Comparator

TP17 PLT Comparator
TP18 LMNE CIS Comparator
3-2
Electric &
Electronic Principles
TEST POINT
DESIGNATION
POTEN-
TARGET
TP40 5V Power supply

5V
12V
24V
TP43 -12V Power supply

TP44
GROUND
TP45
GROUND
-12V
TP46
GROUND
TP47
GROUND
TP48 LMNE Draining sensor
TOLERANCE
REMARK
TIOMETER
R286
<1V
With water
R287
R11
<1V
6V
With water
R409
50s
+/- 2s
Factory Adjusted
R410
250s
+/- 5s
Factory Adjusted
TP49
UNUSED
TP50 Reagent heating system CTN voltage
TP51 Thermostated room heating sytem CTN voltage
TP52 Draining sensor
TP53 LMNE Lamp
TP54
GROUND
TP55
TP56
TP57
GROUND
GROUND
GROUND
TP58
GROUND
TP59
GROUND
TP60 LMNE Resistive line
reject2 adjustment
TP61 LMNE Resistive line
reject2 adjustment
3-3
Pentra 60 C+
2. MAIN BOARD POTENTIOMETERS
POTENTIOMETER
DESIGNATION
TEST
POINT
TARGET
TOLERANCE
REMARK
R133 RBC line height adjsutment
TP20
F.A.
R134 BASO gain adjustment

R135 PLT line height adjustment
R136 LMNE Resistive gain adjustment
TP21
TP22
TP23
F.A.
F.A.
F.A.
R139 BASO line reject adjustment

R142 RBC line reject adjustment
R144 PLT line reject adjustment
TP29
TP26
TP32
F.A.
F.A.
F.A.
R145 LMNE Resistive line reject adjustment

R147 LMNE Optical line reject adjustment
R148 LMNE Optical gain adjustment
TP35
TP38
TP24
F.A.
F.A.
F.A.
R149
R150
R151
R152
TP5
TP6
TP7
TP8
4.8V
4.8V
4V
3V
+/- 0.05V
+/- 0.05V
+/- 0.05V
+/- 0.05V
R154 Horizontal carriage motor

R155 Sample syringe motor
R156 Needle carriage motor
TP10
TP11
TP12
3V
2V
4.5V
+/- 0.05V
+/- 0.05V
+/- 0.05V
R157 BASO Threshold adjustment

R158 RBC Threshold adjustment
R159 PLT Threshold adjustment
TP14
TP13
TP2
300mV
300mV
300mV
+/- 5mV
+/- 5mV
+/- 5mV
R160 LMNE CIS Threshold adjustment

R161 LMNE OD Threshold adjustment
R248 HB Gain adjustment
TP3
TP4
TP1
650mV
350mV
4.7mV
+/- 5mV
+/- 5mV
+/- 0.1mV
Draining syringe motor

Counting syringe motor
Dilutor syringe motor
Injection syringe motor
3-4
Electric &
3. PENTRA 60 C+ BOARDS
3.1 Main board XAA 425 C
3-5
Pentra 60 C+
3.2. Optical Preampli board XAA 423 B
OPTICAL PRE-AMPLI BOARD:

Perform an absorbance measurement
This is an Intensity / Tension convertissor
A photodiode measures absorbance of light through the cell, the optical pre-ampli board
returns an intensity proportionnal to the light signal (Light signal is proportionnal to the cell
size)
3-6
Electric &
3.3. Motor relay board XAA 427 B
MOTOR RELAY BOARD:

Interconnection board where all the different motors and end of run sensor are connected
This board is connected to the main board with a flat cable
3-7
Pentra 60 C+
3.4. Carriage board XAA 428 C
CARRIAGE BOARD:
Interconnection board where all the carriage electrical functions are connected
This board is connected to the main board with a flat cable
3-8
Electric &
3.5. LED board XAA 429 A
LED BOARD:
This board supports LEDs on the front panel. They give instrument state:
Red: instrument is busy
Green: instrument is ready to sample
3-9
Pentra 60 C+
4. PENTRA 60 C+ CONNECTORS
4.1. Chamber Heating Connector XBA 386 A
3-10
Electric &
4.2. Reagent Heating Coil Connector XBA 387 A
3-11
Pentra 60 C+
4.3. Horizontal Carriage Motor XBA 391 A
3-12
Electric &
4.4. Upper Fan XBA 393 A
3-13
Pentra 60 C+
4.5. IR sensor XBA 394 AS
3-14
Electric &
3-15
Pentra 60 C+
3-16
Electric &
3-17
Pentra 60 C+
4.9. RBC / WBC coaxe XBA 398 B
3-18
Electric &
4.10. LMNE flowcell coaxe XBA 399 AS
3-19
Pentra 60 C+
4.11. Barcode reader cable XBA 402 AS
3-20
Electric &
4.12. Diluent level sensor XDA 605 AS
3-21
Pentra 60 C+
5. PENTRA 60 C+ SYNOPTIC
3-22
jhfh
Pentra 60 C+
ANALYSIS CYCLE TECHNOLOGY
1. ANALYSIS CYCLE DESCRIPTION ............................................ 2-2
2. MEASURING PRINCIPLES ...................................................... 2-4
2.1. Multi Distribution Sampling System (MDSS) .................... 2-4
2.2. RBC / Plt detection principles ......................................... 2-5
2.3. Hgb measurement ......................................................... 2-6
2.4. Hct measurement .......................................................... 2-7
2.5. RDW calculation ............................................................. 2-7
2.6. MCV, MCH, MCHC calculation ....................................... 2-7
2.7. MPV Measurement ........................................................ 2-8
2.8. Pct calculation ................................................................ 2-8
2.9. PDW calculation ............................................................. 2-8
2.10. WBC and differential count ........................................... 2-9
2.10.1. General principles .................................................. 2-9
2.10.2. BASO / WBC Count .............................................. 2-9
2.10.3. LMNE matrix ........................................................ 2-10
Pentra 60 C+
1. ANALYSIS CYCLE DESCRIPTION
Aspiration of blood sample from the tube trough the cap (53l)
Translation of sampling carriage over Rinse chamber.
Cleaning of the needles:
Internal cleaning: 1ml inside piercing needle
External cleaning: 2ml outside piercing needle
Internal cleaning: 1ml inside piercing needle and reject of 3l of blood
(not used).
Translation of sampling carriage DIL1 chamber (first dilution), descent of

the needle.
Positioning the needle point into a tangential flux of ABX diluent (1.65ml)
and synchronised distribution of 10l of blood.
Translation of sampling carriage over WBC/BASO count chamber.

Positioning the needle point into a tangential flux of 2ml of ABX BASOLYSE
II and synchronised distribution of 10l of blood.
Dilution
Blood Volume
BASOLYSE II Volume
Dilution Rate
10 l
2000 l
1/200
Translation of sampling carriage over LMNE mixing chamber.

Positioning the needle point into a tangential flux of 1ml of ABX Eosinofix
and synchronised distribution of 25l of blood.
Dilution
Blood Volume
Eosinofix Volume
Diluent Volume
Final Dilution Rate
4-2
25 l
1000 l
1000 l
1/80
Analysis Cycle
Technology
Translation of sampling carriage over Rinse chamber
Rinsing of the needles:
Internal rinsing: 0.8ml inside sampling needle
External rinsing: 0.5ml outside sampling needle
Internal rinsing: 0.5ml inside sampling needle
External rinsing: 0.3ml outside sampling needle
WBC and LMNE counting:

1ml of diluent into LMNE chamber
Dilution transfer trough LMNE flowcell and counting during 12 seconds
WBC\BASO counting
Translation of sampling carriage over DIL1 chamber

Sample of the 42.5l of the first dilution.
Translation of sampling carriage over Rinse chamber.

Rinsing of the exterior of sampling needle with 0.4ml of diluent
Translation of sampling carriage over RBC and PLT count chamber.

Distribution of the 42.5l of first dilution into a flux of 2.20ml of diluent.
Distribution of 0.3ml of diluent from the interior of the needle.
Dilution
Initial Blood Volume
Reagent Volume (RBC chamber)
Final Dilution Rate
10 l
2500 l
1/10000
Hgb:
Distribution of 0.36ml of ABX ALPHALYSE into DIL1 chamber while bubbling.
WBC\BASO chamber rinsing:
Draining and filling with 1ml of ABX CLEANER and 1.5ml of diluent
RBC\Plt counting and Hgb measurement
Chambers cleaning and Hgb blank
Draining and filling of DIL1 chamber with 2.7ml of diluent
Measurement of Hgb blank
Flowcell rinsing (about 1.6ml of diluent)
Draining and filling of RBC chamber with 2.5ml of diluent
4-3
Pentra 60 C+
2. MEASURING PRINCIPLES
2.1. Multi Distribution Sampling System (MDSS)

+ ABX PENTRA 60 C+ analysis
cycle needs
3 blood specimens distributed
as follows:
one specimen for the first
RBC/Plt dilution and the Hgb
measurement.
Diluent
Air bubble
another specimen for the

BASO/WBC count.
Not used
the last specimen for the

LMNE matrix.
LMNE Dilution
These 3 specimens are

provided by means
of a multidistribution
principle:
53 l of blood are aspirated
inside the needle (sufficient
volume for all dilutions) then
divided up and distributed to
the chambers with reagents.
WBC\BASO Dilution
RBC\PLT\HGB first dilution
Not used
Extremities of the specimen

are not delivered to the dilutions.
SPECIMENS INSIDE THE NEEDLE
+ Specimen distribution in
the chambers is carried out in a
tangential flow of reagent
which allows perfect mixing of
the dilution and avoids any
viscosity problems (this multi
distribution in a reagent flow is
an ABX DIAGNOSTICS patent).
Needle
Reagent
input
Tangential Flow Mix

Mixing chamber
BLOOD DISTRIBUTION IN A TANGENTIAL FLOW
4-4
Analysis Cycle
Technology
2.2. RBC / Plt detection principles
+ Measurement of
impedance variation
generated by the passage of
cells through a calibrated
micro aperture.
The specimen is diluted in an
electrolytic diluent (current
conductor) and pulled through
the calibrated micro-aperture.
Two electrodes are placed on
either side of the aperture.
Electric current passes through
the electrodes continuously.
Solution to be ananlyzed
Vacuum = Constant
Volts
Analyzing
electronic
circuit
Electrodes
When the cell passes through

the aperture, electric resistance
between the two electrodes
increases proportionately with
the cell volume.
The generated impulses have
a very low voltage, which the
amplification circuit increases,
so that the electronic system
can analyze them and eliminate
the background noise.
Time
Pulse
TECHNICAL
CHARACTERISTICS OF THE
RED BLOOD CELL
AND
PLATELET
COUNTS
Initial blood volume
10 l
Method
Impedance
Vol. ABX DILUENT

Final dilution rate**
Temperature of reaction
2500 l
1/10000
35C
Aperture diameter
Count vacuum
Count period
50 m
200 mb
2 X 5 seconds
**: TWO SUCCESSIVE DILUTIONS ARE CARRIED

PRIMARY DILUTION FOR RBC AND PLT:
Blood (l):
10
ABX DILUENT (L):
1700
SECONDARY DILUTION RBC
Dilution (l): 42.5

ABX DILUENT(L):
FINAL
DILUTION:
AND
2500
OUT
dilution:
PLT (FROM
1/170
THE PRIMARY DILUTION):
dilution:
1/170 X 1/58.8 =1/10000
4-5
1/58.8
Pentra 60 C+
Results
RBC
Number of cells counted per volume unit x Calibration coefficient.
Histograms
RBC: Distribution curves on 256 counting channels from 30fl to 300fl.

Plt: Distribution curves on 256 channels from 2fl to a mobile threshold. This threshold
moves according to the microcyte population present in the analysis area.
2.3. Hgb measurement

The hemoglobin released by the lysis of the red blood cells combines with the
potassium cyanide to form the chromogenous cyanmethemoglobin compound.
This compound is then measured through the optical part of the first dilution
chamber using a spectrophotometric technique at a wavelength of 550 nm.
TECHNICAL
CHARACTERISTICS FOR THE MEASUREMENT OF THE
Volume of blood
Volume ABX DILUENT
10 l
1700 l
Volume ABX ALPHALYSE

Complement ABX DILUENT
Final dilution rate
400 l
400 l
1/250
Temperature of reaction
35C
Method
Wavelength
HEMOGLOBIN:
Photometry
550 nm
Result
Final Hgb result represents: Absorbance value obtained x coefficient of calibration.
4-6
Analysis Cycle
Technology
2.4. Hct measurement

The height of the impulse generated by the passage of a cell through the microaperture is directly proportional to the volume of the analyzed RBC.
The hematocrit is measured as a function of the numeric integration of the MCV.
2.5. RDW calculation

The study of the RBC distribution detects erythrocyte anomalies linked to
anisocytosis.
A Red Cell Distribution Width (RDW) will enable you to follow the evolution of the
width of the curve in relation to the cell number and average volume.
K SD
RDW=
MCV
With:
K = system constant
SD = Determined standard deviation according to statistical studies on cell distribution.
MCV = Mean Corpuscular Volume of erythrocytes
2.6. MCV, MCH, MCHC calculation

MCV (Mean Cell Volume) is calculated directly from the RBC histogram.
MCH (Mean Cell Hemoglobin) is calculated from the Hgb value and the RBC
number.
The mean hemoglobin weight in each RBC is given by the formula:
Hgb
MCH (pg) =
x 10
RBC
MCHC (Mean Corpuscular Hemoglobin Contained) is calculated according to

the Hgb and Hct values. Mean Hgb concentration in the total volume of RBC is
given by the formula:
Hgb
MCHC (g/dL) =
x 100
Hct
4-7
Pentra 60 C+
2.7. MPV Measurement

The MPV (Mean Platelet Volume) is directly derived from the analysis of the
platelet distribution curve.
2.8. Pct calculation

Thrombocrit is calculated according to the formula:
Plt (103/l) x MPV (m3)
Pct% =
10 000
2.9. PDW calculation

PDW (Platelet Distribution Width) is
calculated from the Plt histogram.
The PDW is represented by the width
of the curve between
15% of the number of platelets
starting from 2 fl (S1),
and 15% of the number of platelets
beginning with the variable
top threshold (S2).
S2
S1
PDW
4-8
Analysis Cycle
Technology
2.10. WBC and differential count
2.10.1. General principles
The WBC count is carried out twice by two different sensors:
In the BASO count chamber at the same time as the BASOS count,
In the optical chamber during the acquisition of the LMNE matrix.
The reference count is the one obtained in the WBC and BASO count chamber.
2.10.2. BASO / WBC Count

Detection principle is the same as for RBC.
Differentiation betwen BASOs and other leukocytes is obtained by means of the
BASOLYSE II specific lysing action.
All the WBCs are counted between the electrical threshold <0> threshold <BA3>.
The basophils are located from threshold <BA2> to threshold <BA3>.
BA1
BA2
BA3
WBC
BASO
TECHNICAL CHARACTERISTICS
OF THE
WBC/BASO
COUNT
Blood volume
BASOLYSE II volume
Dilution rate
10 l
2000 l
1/200
Method
Ruby diameter
Depression of count
Impedance
80 m
200 mb
Reaction temperature
35 C
Duration of the count
2 X 6 seconds
Results
WBC: Number of cells per volume x coefficient of calibration.

BASO: Number of cells per volume x coefficient of calibration in percentage
regarding the total number of leukocytes (BASO + WBC nuclei)
4-9
Pentra 60 C+
2.10.3. LMNE matrix
The WBC and Differential count

are based on 3 essential
principles:
2) SECOND FOCUSED FLOW FOR OPTICAL

DETECTION
1- The double
hydrodynamic sleeving
DHSS (ABX DIAGNOSTICS
patent)
2- The volume
measurement
impedance changes.
3- The measurement of
transmitted light
with 0 angle, which permits a
response according to the
internal structure of each
element and its absorbance by
means of incident light diffusion.
25l of whole blood is
delivered to the LMNE
chamber in a flow of
EOSINOFIX. This reagent lyses
the RBC, stabilizes the WBC in
their native forms and stains
the eosinophil nuclei with a
specific coloration.
The solution is then stabilized
with diluent and transferred to
the measuring chamber. Each
cell is measured both in
absorbance (cytochemistry)
and resistivity (volume).
1) PRIMARY FOCUSED FLOW FOR IMPEDANCE

MEASUREMENT
TECHNICAL
CHARACTERISTICS OF THE
WBC
COUNT DURING THE ACQUISITION OF
THE MATRIX
Blood volume
25 l
Method
Impedance
with hydrofocus
Eosinofix volume
Diluent volume
Final dilution rate
1000 l
1000 l
1/80
Ruby diameter
Flow diameter
Injection duration
60 m
42 m
12 seconds
Reaction temperature
Incubation duration
35 C
12 s.
Volume injected
72 l
Results
From these measurements, a matrix is drawn up with volumes on the X-axis and
optical transmission on the Y-axis.
The study of the matrix image permits the clear differentiation of 4 out of 5 leukocyte
populations. As a matter of fact, the basophil population is very small compared
to the other 5 in a small blood sample.
4-10
Analysis Cycle
Technology
ABSORBANCE
NEUTROPHILS
EOSINOPHILS
LMNE
LIC
VOLUME
LYMPHOCYTES
ALY
4-11
MONOCYTES
Pentra 60 C+
MONOCYTES: The monocytes, being cells with large kidney shaped nuclei and a large non-granular
cytoplasm, will neither be scattered nor absorb a large amount of light. They will therefore be positioned
in the lower part of the optical axis but clearly to the right of the volume axis. Certain large monocytes can
be found on the right side of the matrix in the lower LIC (Large Immature Cells) zone. The immature
granulocytic cells are detected by their larger volumes and by the presence of granules which increase
the intensity of the scattered light. Therefore, cells such as metamyelocytes will be found clearly to the
right of the neutrophils and nearly at the same level. Myelocytes and promyelocytes will be found in
saturation position on the far right of the matrix. These last three populations will be counted as LIC (Large
Immature Cells) and their given results are included in the neutrophil value. The blast cells will be found
generally to the right of the monocytes, and, as such, will increase the LIC count. Small blasts will be
found between the normal lymphocytes and monocytes. Platelets and debris from erythrocyte lysis
represent the background noise population located in the lower left area of the matrix. Most of the population partition thresholds are fixed and give the limits of the morphological normality of leukocytes. Changes in the morphology of a population will be expressed on the matrix by a shifting of the corresponding
population.
LYMPHOCYTES: The lymphocytes being small with regular shape, are positioned in the lower part of
both the optical axis and volume axis. Normal lymphocyte populations are generally observed with a
good volume homogeneity. Large lymphocytes are detected in the ALY (Atypical Lymphocytes) zone,
where reactive lymphoid forms, stimulated lymphocytes and plasmocytes are also to be found. The far
left side of the lymphocyte zone should normally be empty, but when small lymphocytes are present,
population may exist in this area. The presence of platelet aggregates is detected by a distribution pattern
that moves from the origin of the matrix (background zone) into the lymphocyte zone. The NRBCs with
their cytoplasmic membranes lysed like the erythrocytes, will have their nuclei situated to the far left side
of the lymphocyte zone.
EOSINOPHILS: With reagent action on cytoplasmic membranes, the leukocytes keep their native size
and only eosinophils are colored for optical separation. Eosinophils will be situated in the upper part of the
optical Y-axis due to their strong absorbance qualities and their size, which is nearly equivalent to large
neutrophils.
NEUTROPHILS: The neutrophils, with their cytoplasmic granules and their generally segmented nuclei,
will scatter light depending on their morphological complexity. A hypersegmented neutrophil will give an
increased optical response with respect to a young neutrophil population which will be in the upper
position of the optical axis depending on the presence of segmentation and/or granules.
4-12
Pentra 60 C+
SOFTWARE VERSIONS
1. SERVICE SOFTWARE OVERVIEW ........................................... 5-2
2. SOFTWARE RELEASES .......................................................... 5-3
2.1. Software releases table .................................................. 5-3
2.2. Software modifications ................................................... 5-3
Pentra 60 C+
1. SERVICE SOFTWARE OVERVIEW
MECHANICAL SYSTEMS
+ Initialization
+ Check motors
+ Check valves
+ Sampling needle adjustment
+ Maintenance carriage position
+ Park syringe position
HYDRAULIC SYSTEMS
+ Drain chamber
+ Rinse
+ Cleaning cycle
+ Prime cycles
TECHNICIAN ADJUSTMENT
+ Sampling needle adjustment

+ Draining sensor adjustment
+ Transfer sensor adjustment
+ Vacuum adjustment
+ Cycles
+ Heating coil adjustment
+ Thermostatic compartment adjustment
+ Bublbing
+ Carriage dilution positions
TECHNICIAN MEASUREMENT
+ Gain adjustment
+ LMNE calibration
+ Pulse adjustment
BURN IN CYCLE
+ Burn in parameters
RS232 TO P60
+ Connection test & command

+ Service mode
+ Configure Analyzer Main board
5-2
Software Versions
2. SOFTWARE RELEASES
2.1. Software releases table

TECHNICAL NOTE
RAH801A
RAN015A
SOFTWARE REVISION
DATE
V1.61
V2.2.9
14/04/2001
15/02/2004
2.2. Software modifications

Between software released, the software or techchnical evolutions may be described in
Technical notes.
5-3
Pentra 60 C+
OUTPUT FORMAT
Refer to document ABX Diagnostics OUTPUT FORMAT RAA024
Pentra 60 C+
ALARMS & ERROR LIST
1. TRANSMISSION ...................................................................... 7-2
2. DATABASE ............................................................................. 7-2
3. WORKLIST ............................................................................. 7-2
4. CALIBRATION ........................................................................ 7-3
5. TECHNICAL OPERATIONS ..................................................... 7-3
6. PRINTER ................................................................................ 7-3
Pentra 60 C+
1. TRANSMISSION
DISPLAYED
MESSAGE
CAUSES
ACTION
Defect on transmission
operations
Check RS232 setup

Menu\Service\RS232 TO P60
Time out
Disconnect/Reset/Connect
from menu RS232 TO P60
No ENQ Character Received On RS232

No ACK Character Received On RS232
Write Error On RS232
Internal Error On RS232
Time Out Reached
Too Many Characters Received On RS232
Connection To Analyser Failed
Communication to S.I.L. : Spooler full, result lost !

Result transmission capacity exceeded, only the first XX will be sent
Connection Failure - - - - - -
2. DATABASE
DISPLAYED
MESSAGE
Similar Reference Found In The Database

Result Not Recorded, File Full
Result Not Recorded, Write Error In Database
Insufficient free disk space !
There Is No Valid Backup
Abnormal Backup Termination !
Abnormal Restore Termination !
File XX Not Found In YY Directory
3. WORKLIST
DISPLAYED
MESSAGE
Sample Id Already Exists In Worklist !

Rejected Result Not Recorded
Invalid Date
Invalid Gender Entry
Patient Name Field Cannot Be Empty !
Sample Id Field Cannot Be Empty !
Patient Information Cannot Be Modified, Result Already Exist !
7-2
Alarms &
Error list
4. CALIBRATION
DISPLAYED
MESSAGE
Minimum Of 3 Results Required For Calibration

Calibration Failed : Confirm To Force Calibration
Calibration Failed : Coef. Out Of Range
Minimum Of 5 Results Required To Calibrate
5. TECHNICAL OPERATIONS
DISPLAYED
MESSAGE
Values Out Of Range : [XX]

YY Value Out Of Range [XX - ZZ]
YY Value Cannot Be Less Than XX Value
YY Value Cannot Be Less Than 1
YY Value Must Be Positive
Access denied
Invalid Value(s) On XX Pathological Limits
Thresholds not saved. - - - - Acceptance conditions violated:
6. PRINTER
DISPLAYED
MESSAGE
Are windows messages
7-3
Pentra 60 C+
MAINTENANCE
1. MAINTENANCE ......................................................................
1.1. Introduction .....................................................................
1.2. Daily customer maintenance ..........................................
1.3. Weekly customer maintenance ......................................
1.4. Maintenance table ..........................................................
8-2
8-2
8-2
8-2
8-3
2. MAINTENANCE KITS .............................................................

2.1. 6 month maintenance kit ................................................
2.2. Yearly maintenance kit ...................................................
2.3. Pistons maintenance kit .................................................
2.4. Fitting kit .........................................................................
2.5. Screws kit ......................................................................
8-4
8-4
8-4
8-5
8-5
8-6
3. PROCEDURES .......................................................................
3.1. Required tools and product ............................................
3.2. Procedure chart table ....................................................
3.3. Procedure chart table in maintenance order ..................
8-7
8-7
8-8
8-9
Pentra 60 C+
1. MAINTENANCE
1.1. Introduction
WARNING
Customer maintenance has to be carried out according to the recommended
frequency chart table and after having attended an ABX approved customer training course.
The system warranty may be affected if damage occurs after a non trained technician
intervenes or if replaced spare parts and consumables do not come from an ABX
approved origin.
1.2. Daily customer maintenance

No special adjustments or maintenance has to be done on your equipment if the
recommended startup and shutdown procedures are explicitly respected.
See the ABX PENTRA 60 C+ User Manual for the daily rinsing and cleaning of the system.
1.3. Weekly customer maintenance

An overall check for cleanliness of the system is recommended every week.
All traces of blood or reagent have to be wiped off as soon as possible using a piece of cloth
and distilled water.
CAUTION
Never use solvent or abrasive cleaning material to clean the system.
8-2
Maintenance
1.4. Maintenance table
The ABX PENTRA 60 C+ maintenance is scheduled on 3 different maintenance kits. The
maintenance schedule depends on the number of cycles per day, follow the chart table to
know the kit part number to use:
CYCLES PER DAY -
<30
MAINTENANCE EACH - 1Y
Sampling probe Oring
2Y
X
30 to 120
6M 1Y
X
>120
18M
2Y
6M
1Y
PART NUMBER
KIT TO USE
XEA710BS
Reagent syringe Oring
Draining and Counting syringes

Oring
Sampling syringe
5DIFF syringe
Draining chamber
Procedure:
RAS307
XEA486BS
Optical bench lamp
LMNE flowcell coaxial
Diluent reservoir joint
Counting heads
Sampling probe replacement
Procedure:
RAS308
X
XEA630CS
Reagent pistons replacement
Counting and Draining syringe

pistons replacement
8-3
Procedure:
RAS309
Pentra 60 C+
2. MAINTENANCE KITS
2.1. 6 month maintenance kit

KIT XEA710BS
P/N
DESIGNATION
FAA013A
FAA055A
FAA057A
FAA065A
XDA621A
XDA622A
XEA019A
Oring 1.80x1.20 Nitrile (Rinsing block)

Oring 1.07x1.27 Fluorocarbon (Rinsing block)
Oring 5x1 Nitrile (Rinsing block)
Oring 6.30x2.40 Silicon (Reagent syringe)
Oring 30.80x3.60 + Washer (Vacuum/Waste pump)
Oring 15.54x2.62 + Washer (Reagent syringe)
Silicon grease
QTY
1
1
1
1
2
4
1
2.2. Yearly maintenance kit

KIT XEA486BS
P/N
DESIGNATION
DAJ007A
FAA040A
FAA046A
FAA064A
FAA066A
FAA067A
GBG275A
JAJ007A
XEA286AS
XEA663A
JAJ007A
9.5V 20W lamp

Oring 12.10x2.70 Silicon
Oring 2.75x1.60 Viton
Oring 1.42x1.52 Fluorocarbon
Flat EPO joint 0.5
Polyethylene ribbon
Waste chamber Joint + Washer
Coaxial kit LMNE flowcell
Polyethylene tape
8-4
QTY
1
1
2
2
4
4
4
1m
1
1
1m
Maintenance
2.3. Pistons maintenance kit

KIT XEA630CS
P/N
DESIGNATION
GBC030A
GBC031A
GBG169A
GBG260A
GBG274A
GBG288A
KAA009A
Reagent syringe piston

Sampling carriage percutor
31mm piston spacer
31mm piston
Sampling carriage
CHC M3 screw
QTY
4
1
1
2
2
1
6
2.4. Fitting kit

The XEA311 kit contains the most common pneumatic and hydraulic parts.
KIT XEA311
P/N
DESIGNATION
EAA005A
EAA006A
EAA009A
EAB002A
EAB003A
EAB005A
EAB006A
EAB009A
EAB010A
EAA013A
EAA014A
EAB026A
EAB021A
EAB035A
EAB014A
Straight connector 1.6mm 1/8

Straight connector
Bent connector I=1.5 10/32
L Connector
Connector 4mm H19.5
T Connector 1.6mm T10-6
T Connector 2.3mm
Straight connector 1.6mm
Straight connector 2.3mm
Bent connector 1/8
Straight connector 1/8
Y Connector 2.5mm
Y Connector 3mm
T Connector 2.3mm T220-6
Straight connector Y 1.5mm/1.5mm
8-5
QTY
10
10
10
10
20
30
30
30
30
10
10
3
3
10
10
Pentra 60 C+
2.5. Screws kit
The XEA293 kit contains the most common screws used in ABX PENTRA 60 C+:
KIT XEA293 AS
P/N
DESIGNATION
KAA002A
KAA003A
KAA005A
KAA006A
KAA009A
KAA011A
KAA015A
KAA016A
KAA017A
KAA021A
KAA028A
KAB002A
KAB003A
KAB004A
KAB005A
KAC002A
KAE003A
KAH001A
KAH002A
KAH003A
KAH024A
KAJ001A
KAJ002A
KAJ003A
KAJ007A
KAJ008A
KAJ009A
KAJ010A
KAM002A
KAM003A
KAM004A
KAM005A
KAM006A
KAM010A
KAM011A
KAM013A
KAM017A
KAB016A
CHC M3x6 Screw

CHC M3x8 Screw
CHC M3x12 Screw
CHC M3x14 Screw
CHC M3x20 Screw
CHC M3x30 Screw
CHC M4x8 Screw
CHC M4x12 Screw
CHC M4x16 Screw
CHC M5x10 Screw
CHC M4x20 Screw
FHC M3x6 Screw
FHC M3x8 Screw
FHC M3x10 Screw
FHC M3x12 Screw
TC M3x5 Screw
BHC M4x12 Screw
HU M3 Nut
HU M4 Nut
HU M5 Nut
Nut 1/4-28 (US Std)
M Washer Diam.3
M Washer Diam.4
M Washer Diam.5
AZ Washer Diam.3
AZ Washer Diam.4
AZ Washer Diam.5
W Grower Washer Diam.5
Plastic Crosspiece M3x6 Lg.8
Plastic Crosspiece M3.5x6 Lg.6
Plastic Crosspiece M3.5x6 Lg.5.7
Brass Crosspiece M3.5x6 Lg.3
FX M3x8 Screw
8-6
QTY
20
20
20
20
20
20
20
20
20
20
20
20
5
20
20
20
20
20
20
20
10
20
20
20
20
20
20
20
10
10
10
10
10
10
10
10
10
5
Maintenance
3. PROCEDURES
NOTE
Maintenance and adjustments required for ABX PENTRA 60 C+ are
divided into procedures according to concerned assemblies. This
should make any update easier as all interventions can be done with
the corresponding procedure on its own.
Each procedure has to be read entirely before starting intervention:
RED Procedure concerns an Adjustment
BLEU Procedure concerns Maintenance
GREEN Procedure concerns one Dismantling
WARNING
Procedures must be performed on a clean instrument.
If the instrument is suspected not to be perfectly clean, perform a
Concentrated cleaning. Disposable gloves should be worn.
3.1. Required tools and product

TOOLS / PRODUCTS
DESIGNATION
HEXAGONAL KEYS
EMPTY SAMPLE TUBES
DYNAMOMETRIC SCREW DRIVER A302
SILICONE GREASE
GREASE FOR MECHANICAL ASSEMBLIES
SOFT TISSUE
CLAMPS
LIQUID SOAP
SCALPEL
DISTILLED WATER
CUTTING PLIERS
MICROPIPETTE TIP
PAIR OF SCISORS
FLAT PIECE OF STIFF PLASTIC
VOLTMETER
SYRINGE 5ML
FLAT SCREWDRIVER
LATEX RBC
BARFLEX
FELT PEN
THERMOMETER
TORX KEYS
8-7
P/N
MAG019A
LAM004A
MAG020A
XEA381AS
MAG013
LAD002AS
Pentra 60 C+
3.2. Procedure chart table
P/N
PROCEDURE
RAS290
PROBE ADJUSTMENT
RAS297
RAS298
RAS299
RAS300
RAS303
RAS305
RAS306
RAS307
RAS308
RAS309
RAS310
RAS311
RAS312
RAS313
RAS314
RAS315
RAS316
RAS317
RAS318
RAS319
RAS320
CONCERNS
Probe Home position Check and Adjustment - Probe position Check

and Adjustment
CHANGEMENT OF KEYBOARD TYPE
INSTALLATION
Unpacking - Working conditions - General description - Printer &
instrument connection - Reagent connections - Priming & Startup
CHECK UP AFTER
Instrument check up: Repeatability - Calibration
INTERVENTION
TUBE HOLDER ADJUSTMENT
LMNE BALANCE ADJUSTMENT
TEMPERATURE ADJUSTMENT Reagent heating system temperature Check - Reagent heating system
temperature Adjustment - Thermostatedcompartment temperature
Check - Thermostated compartment temperature Adjustment
FRONT PANEL DISMANTLING Front panel Dismantling - Front panel Mounting
6 MONTH MAINTENANCE
Sampling Probe: O'ring Replacement - Reagents syringe: O'rings
Replacement - Vacuum/Waste pump: O'rings Replacement
YEARLY MAINTENANCE
Sampling syringe: O'ring Replacement - 5DIFF syringe: O'ring
Replacement - Draining chamber: O'ring Replacement - Optical Bench:
Lamp & LMNE flowcell coaxial Replacment - Diluent reservoir: Joint
and washer Replacement - Counting Heads: RBC/WBC Coaxial, O'ring
and Aperture joint Replacement
EVERY 2 YEARS
Sampling probe: Probe & Guide Replacement - Reagents syringe:
MAINTENANCE KIT
Pistons Replacement - Vacuum/Waste pump: Piston Replacement
DECONTAMINATION & RINSE Instrument Decontamination & Rinse
LMNE FLOWCELL
Optical Bench Check and Adjustment
ADJUSTMENT
CHAMBERS ADJUSTMENT
Alignment Check and Adjustment of chambers assembly
MAIN BOARD ADJUSTMENT
HGB Blank Adjustment - RBC/PLT Gain Adjustment - WBC/BASO Gain
Adjustment - Drain sensor Adjustment - LMNE sensor Adjustment Motor Current Adjustment - Thresholds Adjustment
VACUUM ADJUSTMENT
Draining syringe vacuum Check - Counting syringe vacuum Check Counting syringe vacuum Adjustment
BUBBLING ADJUSTMENT
Bubbling Adjustment Menu presentation
STEP BY STEP ANALYSIS CYCLE
OPTICAL BENCH DISMANTLING Optical bench Dismantling - Optical bench Replacement - Emission
& REPLACEMENT
gun location Control - Optical bench lamp alignement Control
BARCODE READER
Instrument configuration for Barcode reader
CONFIGURATION
POWER SUPPLY REPLACEMENT
HEATER ASSY REPLACEMENT
8-8
Maintenance
3.3. Procedure chart table in maintenance order
P/N
PROCEDURE
RAS298
INSTALLATION
RAS307
RAS308
RAS309
RAS310
RAS312
RAS290
RAS313
RAS305
RAS314
RAS315
RAS311
RAS303
RAS300
RAS319
RAS299
RAS317
RAS306
RAS318
RAS320
RAS301
RAS297
RAS316
CONCERNS
Unpacking - Working conditions - General description - Printer &

instrument connection - Reagent connections - Priming & Startup
6 MONTH MAINTENANCE
Sampling Probe: O'ring Replacement - Reagents syringe: O'rings
Replacement - Vacuum/Waste pump: O'rings Replacement
YEARLY MAINTENANCE
Sampling syringe: O'ring Replacement - 5DIFF syringe: O'ring
Replacement - Draining chamber: O'ring Replacement - Optical Bench:
Lamp & LMNE flowcell coaxial Replacment - Diluent reservoir: Joint
and washer Replacement - Counting Heads: RBC/WBC Coaxial, O'ring
and Aperture joint Replacement
EVERY 2 YEARS
Sampling probe: Probe & Guide Replacement - Reagents syringe:
MAINTENANCE KIT
Pistons Replacement - Vacuum/Waste pump: Piston Replacement
DECONTAMINATION & RINSE Instrument Decontamination & Rinse
CHAMBERS ADJUSTMENT
Alignment Check and Adjustment of chambers assembly
PROBE ADJUSTMENT
Probe Home position Check and Adjustment - Probe position Check
and Adjustment
MAIN BOARD ADJUSTMENT
HGB Blank Adjustment - RBC/PLT Gain Adjustment - WBC/BASO Gain
Adjustment - Drain sensor Adjustment - LMNE sensor Adjustment Motor Current Adjustment - Thresholds Adjustment
TEMPERATURE ADJUSTMENT Reagent heating system temperature Check - Reagent heating system
temperature Adjustment - Thermostatedcompartment temperature
Check - Thermostated compartment temperature Adjustment
VACUUM ADJUSTMENT
Draining syringe vacuum Check - Counting syringe vacuum Check Counting syringe vacuum Adjustment
BUBBLING ADJUSTMENT
Bubbling Adjustment Menu presentation
LMNE FLOWCELL
Optical Bench Check and Adjustment
ADJUSTMENT
LMNE BALANCE ADJUSTMENT
TUBE HOLDER ADJUSTMENT
POWER SUPPLY REPLACEMENT
CHECK UP AFTER
Instrument check up: Repeatability - Calibration
INTERVENTION
OPTICAL BENCH DISMANTLING Optical bench Dismantling - Optical bench Replacement - Emission
& REPLACEMENT
gun location Control - Optical bench lamp alignement Control
FRONT PANEL DISMANTLING Front panel Dismantling - Front panel Mounting
BARCODE READER
Instrument configuration for Barcode reader
CONFIGURATION
HEATER ASSY REPLACEMENT
WORKSTATION SOFTWARE
UPGRADE
CHANGEMENT OF KEYBOARD TYPE
STEP BY STEP ANALYSIS CYCLE
8-9
Probe adjustment
Procedure
CONCERNS
Check and adjsutment of the probe
None
RAS290B
REQUIRED PRODUCTS
None
INTERVENTION TIME
15 min.
15/02/04
FREQUENCY
On request
SPECIFIC KIT OR CONSUMABLES

None
Pentra 60C+
REQUIRED TOOLS
Probe adjustment
Probe adjustment
Introduction
Needle home check & adjustment
Carriage home adjustment
All carriage positions check & adjustment
WARNING
Pentra 60C+
Disposal gloves and white coat must be worn by the operator.

Local or national regulations must be applied in all the operations.
Page 2/8
RAS290B
Probe adjustment
Introduction
IMPORTANT
Check that the chamber assy is parallel to the carriage motion
before checking or making an adjustment of the Probe position.
Follow CHAMBERS ADJUSTMENT procedure of this manual.
Most of the probe adjustments can be accessed by the menu Service\Technician

Adjustment\Carriage dilution positions as explained below (Diag.1):
Check (2): Checks the

deepness of the probe in
front of LMNE-3 inlet
Check (1): Allows to

check probe cleaning
position
Carriage Home: Allows adjustment of

the carriage sampling position
Needle position: Probe deepness

adjustment (Up/Down)
All Carriage pos.: Probe Left/Right

adjustment
Diag.1
Probe adjustment
Needle Home: Adjustment to get the

best probe extremity cleaning
Check cycle: Allows to check that probe

is aligned with each distribution inlets
IMPORTANT
Check Probe position before any adjustment, running Check
functions.
Page 3/8
Probe adjustment
Needle home check & adjustment
IMPORTANT
When the probe is in NEEDLE HOME position it is also in cleaning
position. Check and adjust the NEEDLE HOME position to achieve
the best cleaning of the probe extrimity
1. Needle Home Check

Open the right access door.
Run Service\Technician Adjustment\Carriage dilution positions\Check (1) (Diag.1).
Pentra 60C+
Check that the probe is about 1mm out from the percutor (piercing needle) (Diag.2).
Diag.2
Press ESC to continue.
2. Needle Home Adjustment

Run Service\Technician Adjustment\Carriage dilution positions\Check (1) (Diag.1).
Check that the probe is about 1mm out from the percutor (Diag.2).
If Probe is too low (gap>1mm) increase Needle Home number of step.
If Probe is too high (gap<1mm) decrease Needle Home number of step.
Restart the Workstation.
Run the Check (1) cycle again to control the gap.
Page 4/8
RAS290B
Carriage home adjustment
IMPORTANT
The CARRIAGE HOME position is a factory made adjustment and
must not be changed. It represent a gap of 1.4mm between the tooth
wheel and the front of the carriage (as shown on Diag.3)
This carriage position is also the sampling position, if you need to
readjust the sampling position move the tube holder, but do not
change the CARRIAGE HOME value !
Probe adjustment
1.4mm
Diag.3
NOTE
Workstation may have to be restarted after a HOME adjustment.
Page 5/8
Probe adjustment
All carriage positions check & adjustment
IMPORTANT
To check the right position of the probe you must see inlet as in
Diagram 4 and not as in diagram 5 (where circles are not concentric).
Diag.4
Diag.5
1. All Carriage Positions Check
Pentra 60C+
Open the right access door

Run Service\Technician Adjustment\Carriage dilution positions\Check cycle (Diag.1)
The sampling needle moves over a chamber
Check in front of the inlet the right location of the probe: Disconnect the tube connected to the inlet,
if the probe is well adjusted you will see it as in the following diagram (Diag.6):
Diag.6
Press ENTER key or click
end of the cycle
button, to move to the next chamber and continue until the
When the cycle is ended check that no tube is disconnected
2. All Carriage Positions Adjustment
IMPORTANT
Probe Up/Down adjustment is the same for all the chambers and
cannot be modified from one chamber to another (adjustment by the
NEEDLE POSITION value).
Probe Left/Right position in the chambers can be adjusted
separately for each chamber (except the Rinse chamber).
Open the right access door

Before any adjustment check the probe position in each chamber with the Service\Technician
Adjustment\Carriage dilution positions\Check cycle option (Diag.1)
Page 6/8
RAS290B
3. Left/Right adjustment:
Adjust probe position for all the
chambers separately in the software cycle
order (Diag.7&8)
If you want to modify only one of the position, remind to increase or decrease the next motion with
the same number of steps, not to loose the other adjustments.
OS
P
GE
IA
RR
SO
/B
GB
(2)
L
(3)
(1
L1
)DI
CA
IA
RR
GE
S
PO
R
(4)
BC
R
CA
RIA
CA
MN
O
EP
A
EC
RR
S.
OS
2
4
IA
P
GE
Probe adjustment
Diag.7
Diag.8
Page 7/8
Probe adjustment
The right location for the probe is when you see it through the inlet as in the following diagram
(Diag.9):
Diag.9
Pentra 60C+
When the cycle is ended check that no tube is diconnected
4. Up/Down adjustment:
If the Probe is too high increase Needle position value:
Diag.10
If the Probe is too low decrease Needle position value:
Diag.11
Page 8/8
Changement of keyboard type

Procedure
CONCERNS
Changement of keyboard type.
None
RAS297B
REQUIRED PRODUCTS
None
INTERVENTION TIME
15 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Installation
WARNING
Start the Workstation, press ALT+CTRL+DEL to logon.
In Logon information window open an Administrator session
User name: administrator
Password: xxxxx
IMPORTANT
Do not give the administrators password to the customer neither change it.
In case of password modification the system may have to be entirely re-installed
and all datas will be then lost.
Pentra 60C+
Open Start menu in Taskbar and select Run option (Diag.1):
Diag.1
Diag.2
In Run window enter regedt32 (Diag.2).

Select HKEY_USERS on Local Machine(1)window, click over HKEY_USERS(2) directory in this
window (Diag.3):
1
2
Diag.3
Page 2/8
RAS297B
Open Registry menu in Toolbar and select Load Hive option (Diag.4):
Select C:\winnt\profiles\abx directory (Diag.5):
IMPORTANT
Diag.5
There are two files named ntuser.dat select the one with upper case initial.
Select Ntuser.dat(1) file and click Open(2) button (Diag.6):
1
2
Diag.4
Diag.6
Enter abx(1) key name and click OK(2) button (Diag.7):
1
2
Diag.7
Page 3/8
Pentra 60C+
A new profile called abx has been created (Diag.8):
Diag.8
Open .DEFAULT(1)\Control Panel(2)\International(3) directorty and double click on Locale :
REG_SZ : 00000409(4) in the right side of the window, the String Editor window is opened (Diag.9).
Enter keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.9).
NOTE
The new keyboard is the french standard type, code: 0000040c
1
2
3
5
Diag.9
Page 4/8
RAS297B
Open .DEFAULT(1)\Keyboard Layout(2)\Preload(3) directorty and double click on 1 : REG_SZ :
00000409(4) in the right side of the window, the String Editor window is opened (Diag.10).
Enter same keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.10).
NOTE
1
5
2
3
Diag.10
Open abx(1)\Control Panel(2)\International(3) directorty and double click on Locale : REG_SZ :

1
2
4
3
5
6
The new keyboard is the french standard type, code: 0000040c
Diag.11
Page 5/8

Open abx(1)\Keyboard Layout(2)\Preload(3) directorty and double click on 1 : REG_SZ :
4
1
5
2
Pentra 60C+
Diag.12
Close the window session and turn off the computer.
Replace the keyboard, connector is shown on Diag.13:
Diag.13
Restart the computer and start a session under user name abx.
Check that the keyboard operates normally in the workstation application.
NOTE
As the barcode reader is connected on the keyboard, make sure that the
barcode reader works properly after keyboard replacement.
Page 6/8
RAS297B
SPECIAL CASE: Administrator profile
Start the Workstation, press ALT+CTRL+DEL to logon.
In Logon information window open an Administrator session
User name: administrator
Password: xxxxx
NOTE
Open the Control panel window: Start menu Settings\Control panel (Diag.14):
Diag.14
Run the keyboard program manager (Diag.15):
In the Logon information window the keyboard type is still a QWERTY.

To logon with an AZERTY keyboard as an administrator, enter password as if the
keyboard was a QWERTY.
Diag.15
Page 7/8
Pentra 60C+
Enter Input locales tab and select the new keyboard in the list, click Set as Default (Diag.16)
and Apply (Diag.17):
Page 8/8
Diag.16
Diag.17
Click OK to close the Keyboard window.

Restart the Workstation and check the correct operation of the keyboard.
Installation
Procedure
CONCERNS
Installation of the ABX PENTRA 60 C+ and ABX WORKSTATION.
None
RAS298B
REQUIRED PRODUCTS
None
INTERVENTION TIME
10 min
15/02/04
FREQUENCY
On request

Installation kit: XEA602C
Pentra 60C+
REQUIRED TOOLS
Installation
Installation
Instrument installation
Workstation installation
Start Up
Instrument first start up
Check up after intervention
WARNING

Pentra 60C+
INSTALLATION KIT XEA602C

P/N
DESIGNATION
Page 2/6
DAJ007A
DBH001A
DBH002A
DBK003A
EAB021A
EAB033A
EAB035A
EAC010A
EAC019A
EAE005A
EAE006A
EAE007A
EAE008A
EAE011A
EAE028A
EAE034A
EBB057AS
FAA013A
FAA055A
FAA057A
FBH016A
FBL001A
GAK302A
GBG138A
GBG245A
GBG145A
GBG155A
GBG169A
MAB001A
MAB002A
MAB018A
MAB090A
XDA483B
XDA621A
XEA018A
XEA019A
XDA692A
XDA693A
LAMPE 20W 9.5V

TYRAP Lg=92
TYRAP Lg=140
FLAT CABLE ADHESIVE SUPPORT
RACCORD Y =3mm
RACCORD T =1.6mm T410-6
RACCORD T =2.3mm T220-6
LUER FEM. I=3mm
LUER MAL. I=3mm
TYGON TUBE 1.02mm (0.040)
CRISTAL TUBE 3x6
CRISTAL TUBE 4x6
FILTER
ORING 1.8x1.2
ORING 1.07x1.27
ORING 5x1
PENTRA 60 COVER
RUBBER STOPPER (2 HOLES)
STOPPER
1/4 TURN DOOR KEY
REAGENT STRAW STOPPER =28
REAGENT STRAW STOPPER =20
CAPSULE =25
PERCUTOR
ALLEN BENT KEY 2mm
ALLEN BENT KEY 2.5mm
ALLEN BENT KEY 3mm
TORX BENT KEY T10
3v LIQUID VALVE (WITHOUT SELF)
ORING 30.8x3.8 + WASHER
DILUENT STRAW
GREASE
DILUENT INPUT TUBING
WASTE OUTPUT TUBING
QTY
1
3
3
1
1
1
1
2
1
2 ml
2 ml
2 ml
2 ml
2 ml
2 ml
2 ml
1
1
1
1
1
2
3
1
3
1
1
1
1
1
1
1
1
1
1
1
1
1
RAS298B
Installation:
Instrument installation
Unpack the Instrument.
Open the pneumatic door on the right side of the instrument.
Remove the 2 plastic parts blocking the carriage for transportation (Diag.1):
1. Reagent connections
Use Luer connectors, straws and stoppers delivered with the Installation kit to connect Reagents.
Bottles & Containers locations (Diag.2):
1 - ABX ALPHALYSE or ABX LYSEBIO
2 - ABX BASOLYSE II
3 - ABX EOSINOFIX
4 - ABX CLEANER
5 - ABX DILUENT (see connector location on Diag.5).
6 - WASTE CONTAINER (see connector location on Diag.5).
Installation
Diag.1
IMPORTANT
Risk of erroneous results if diluent container
is installed further than 80cm (31.5 in.)
bellow the instrument.
DILUENT INPUT TUBING:

cristal 3x6 / 2 meters (80 in.) maximum.
WASTE OUTPUT TUBING:
cristal 4x6 / 2 meters (80 in.) maximum.
2. Diluent & Waste containers connection
5
Remove the diluent straw.

Install the straw deep in the container.
Diag.2
Page 3/6
Installation
3. Bottle connection
Open the reagent front door.
Open the reagent bottles and replace the cap by the bottle stoppper and reagent straw assembly,
tighten to ensure an adequate seal..
Pentra 60C+
Put the reagent botlles in the compartment and close the door.
Diag.4
Diag.3
4. Electrical connections
Check that the power supply module voltage of the instrument corresponds to the nominal voltage
supply of the laboratory and the country.
Connect the power supply cable (Diag.5).
Connect the RS 232 link to the Worstation (Diag.5).
IMPORTANT
Power requirements:
Analyzer and Computer: 400VA
Printer (EPSON EPL5700): 700VA
+ Workstation connector
+ Main supply cable
+ Diluent input
Cristal tube 3 x 6,
length: 2 meters maximum
+ Waste output
Cristal tube 4 x 6,
length: 2 meters maximum
Diag.5
Page 4/6
RAS298B
Workstation installation
1.Computer installation
Install the workstation computer next to the ABX PENTRA 60 C+.
Check that the power supply module voltage of the computer corresponds to the nominal voltage
supply of the laboratory and the country.
Connect the power supply cable (Diag.6).
Connect the RS 232 link to the ABX PENTRA 60 C+ (Diag.6).
Connect all different peripherals: keyboard, mouse, printer, screen (Diag.6).
+ Printer connector
+ RS Output to LIS
connection
+ Mouse port
Connect standard mouse
to this port.
From 100Vac to 240Vac.
+ Keyboard/
Barcode
connector
Supports standard 105
keys keyboards and
barcode reader.
Diag.6
+ Analyzer connector
+ Screen connector
Connect to ABX PENTRA 60 C+.
SVGA output .
Installation
+ Main supply
Connect the barcode between keyboard and computer:
+ To Computer keyboards input
+ To Keyboard
NOTE
This diagram is given for information, connector locations from one
PC to another may be different.
Page 5/6
Installation
2. Printer installation
The default printer is an OKI 4200 (Printer model may change, refer to latest Technical notes for
informations about new models).
Unpack the printer.
Check that the power supply module voltage of the printer corresponds to the nominal voltage supply
of the laboratory and the country.
Connect the power suppply cable.
Connect the parallel cable.
Pentra 60C+
Feed the printer with paper.
Start Up
Instrument first start up
1. Start Up & Priming
Check that all is correctly connected.
Turn on workstation computer by pressing ON/OFF switch of the computer and screen.
After computer boot press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session.
Start the Analyzer.
Enter system password and wait until workstation starts.
Analyzer indicator turns green if connection is succesfull and reagent levels are updated on the screen.
Prime all the reagents: Menu\Hydraulic Systems\Prime Cycles\ All reagents.
2. Check vacuum value

As it depends on altitude the instrument is installed check the vacuum value. Follow procedure RAS314
and eventually adjust vacuum value.
3. Check up after intervention

Follow procedure RAS299 to check instrument accuracy and eventually re-calibrate it.
Page 6/6

Procedure
CONCERNS
Check up and control of the instrument accuracy
None
RAS299B
REQUIRED PRODUCTS
Fresh and normal blood samples
Calibration blood samples
INTERVENTION TIME
45 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Preliminary
Repeatability
Calibration
WARNING
Pentra 60C+
Page 2/5
RAS299B
Preliminary
WARNING
This procedure must be performed on a clean instrument.
WARNING
After computer boot press ALT+CTRL+DEL to start a session.
Start the Analyzer.
Run a startup, click
button.
Repeatability
The measurement of repeatability is based on the set of 10 results obtained from the consecutive
analyses of the same blood sample.
CBC or DIFF type analyses can be invoked (combination is not supported) with a limit of 30 results per
test. Beyond the 30th result, data generated from a new analysis will be disregarded.
To remain undisturbed the CV calculation, the potential results containing defaults generated directly
from the analyses channels are rejected. In that case, a dialogue box informs the user:
Run several samples in DIFF mode from the Calibration&QC\Repeatability tab and check that
obtained coefficient of variation are within the following limits:
PARAMETERS
WBC
RBC
Hgb
Hct
Plt
%CV
TEST LEVEL
<2%
<2%
<1%
<1%
<5%
at 10x103/L
at 5x106/L
at 15 g/dL
at 45%
at 300x103/L
If the instrument is suspected to not be perfectly clean, perform a

Concentrated cleaning.
Page 3/5

Calibration
In Calibration & Qc(1)\Calibrators(2) Tab, change Calibrator Lot number(3) according to the
Calibrator you are going to use:
Pentra 60C+
2
Diag.1
Prepare the calibrator according to the specific instructions detailed in the calibrator package
insert (temperature, mixing, etc...).
Open the vial and locate it in the tube holder.
In Calibration & Qc(1)\Calibrators(2) Tab close the tube holder.
When the tube holder opens, remove and recap the vial.
IMPORTANT
Risk of erroneous results if the calibrator is not continously mixed between
each analysis. Continue mixing the calibrator between each analysis.
When the analysis cycle ends, the first result is displayed on the result chart table.
Run another calibrator sample when Green LED turns on.
IMPORTANT
In order to obtain the best possible calibration,
it is recommended to run at least 5 calibrator samplings.
The calibration of the ABX PENTRA 60 C+ can be performed on 3 to 11 analyses.

The auto-calculation module performs statistics on these results in order to obtain the best calibration
coefficients.
Page 4/5
RAS299B
Select results to be involved in the statiscal calculation by clicking checked box in Sel(1) column.
To discard a result from the statiscal calculation click again in checked box to remove the mark.
The instrument calculates the statistical calibration factors for each parameter.
Diag.2
If the Coefficient of variation is within the limits setup as described Chapter 4. Instrument configuration
and the percentage difference between the target and the mean value is less than 20, instrument will
allow auto-calibration: Click on
Confirm the changement by clicking
Diag.3
(2) to update new calibration coefficients.

in the following dialog box:
Page 5/5
Tube holder adjustment

Procedure
CONCERNS
XDA654 Tube holder assy replacement
Tube holder mechanical adjustments
Tube holder software adjustment
Tube holder door adjustment
Compatible tube list
Allen keys
Flat screw driver
RAS300B
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

XDA654 Tube holder assy replacement
Tube holder mechanical adjustments
Tube holder parallelism check & adjustment
Tube holder position adjustment (Sampling position)
Centering of the tube retainer plate
Tube holder opening speed adjustment
Introduction
Adjustment
Tube holders door adjustment
Pentra 60C+
Page 2/14
WARNING
WARNING
The probe is a very fragile part, take care during this procedure not
to bend or damage it.
If the teflon part of the needle comes into contact with the tube
holder, the needle will be damaged.
RAS300B
XDA 654 Tube holder assy replacement
Remove the front panel (follow the procedure RAS306 Front panel dismantling).
Unscrew the lower screws from the tube holders support (Diag.1):
Open and remove the tube holder, then unscrew the 2 top screws from the tube holders support
(Diag.2):
Diag.2
Remove the plastic collar (Diag.3) and disconnect all the wires from tube holder:
Diag.3
Diag.1
Unscrew the 3 screws from the top of tube holders support (Diag.4):
Diag.4
Page 3/14

Connect the new tube holder assy as shown below (Diag.5):
SAMPLING POSITION DETECTION SWITCHES
SW3
SW4
SW5
SW6
E10
E12
E14
E16
Pentra 60C+
START CYCLE SWITCH
Page 4/14
ELECTRO-MAGNET COMMAND
E7
Diag.5
E8
SW7
E18
Install the new tube holder assy and tube retainer plate (Diag.1,2&4). Locate the screws but do not
tighten them before you adjust tube holder position.
Replace the plastic collar (Diag.3).
RAS300B
Tube holder mechanical checks & adjustments
Tube holder parallelism check & adjustment
Check tube holder parallelism with a key: Put the key across the tube holder and compare the gap up
and down between the tube holder and the key (Diag.6&7):
Diag.7
NOTE
The gap must be nearly equal all along the tube
holder.
If necessary adjust tube holder parallelism by
untightening the lockers screws (Diag.8) and
adjusting locker.
Diag.8
Once parallelism is adjusted, push backwards the tube holder (closed position) and check presence
of a 1 or 2 mm. play (Diag.9):
IMPORTANT
Diag.6
Without this play the tube holder will

not open correctly.
Diag.9
Page 5/14

If necessary untight the 4 screws (Diag.10-1&11-1) and adjust behind tube holder the tube holder
stopper (Diag.12-7) to get the right adjustment.
Pentra 60C+
Diag.10
Diag.11
Diag.12
Page 6/14
RAS300B
Tube holders position adjustment (Sampling position)
Switch on workstation computer by pressing ON/OFF switch off the computer and screen.
After computer boot, press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session.
Start the Analyzer.
Enter system password and wait for workstation start.
Run an Initialization from Menu:\Mechanical systems\Initialization\Run to put the carriage in
sampling position.
Switch off the Pentra 60C+, remove the tube holder, then untight all the tube holders support screws
(Diag.13,14&17):
Diag.14
Put the tube holder and close the door (beware to the probe).
Push down the probe into one hole, adjust left/right position of the tube holder to center the probe in
the hole by moving the whole tube holder assy (Diag.15):
Diag.13
Diag.15
Lift the probe and open tube holder.

Tight gently the 4 screws (do not tight too much the screws).
Page 7/14

Adjust forward/backward position of the tube holder: Unscrew the tube holders axis to center the
probe in one of the tube holders hole (Diag.15&16):
Diag.16
Pentra 60C+
Tight the axis.
Centering of the tube retainer plate

Untight the 3 screws from the top of the tube holders support (Diag.17):
Diag.17
Adjust the tube retainer plate to be centered with probe (Diag.18):
Diag.18
Tight gently the 3 screws from the top of the tube holders support (Diag.17).
Page 8/14
RAS300B
Tube holders opening speed adjustment
NOTE
This adjustment must done instrument fitted with door and tube
holder.
Switch off the instrument
Close the door and open it, as shown on diagram 16, to control the opening speed:
If necessary untight the screw (Diag.20-1) and adjust the spin to get an acceptable speed:
Faster
IMPORTANT
If opening speed is too fast the tube
risks to be ejected.
Once the adjustment is done, make
1
Diag.20
sure that the screw (Diag.20-1) is well

tightened.
Diag.19
Page 9/14

Introduction
From menu System\Technician Adjustment(1)\Sampling needle adjustment(2) (Diag.21), the
Adjustment function returns the number of steps between the Needle in home position and the top of
the holder:
Pentra 60C+
Diag.21
Adjustment: Returns the number of steps

between the Needle in home position and the
top of the holder.
Control: Check the number of steps between

the Needle in home position and the top of the
holder.
This value is stored in the Tube holder position field in menu System\Mechanical
Systems(1)\Sampling needle adjustment(2) (Diag.22).
1
2
Diag.22
Page 10/14
RAS300B
Adjustment
Switch on workstation computer by pressing ON/OFF switch off the computer and screen.
Start the Analyzer.
Remove the tube holders door (Diag.29).
Close the tube holder.
Enter menu System\Technician Adjustment(1)\Sampling needle adjustment(2) and click
Control(3) button (Diag.23), the needle moves down to the level of a tube position.
.
Diag.23
CAUTION
If the teflon part of the needle comes into contact with the tube holder, the needle
will be damaged.
Instrument running be carefull during intervention.
Gently press and turn the tube holder (Diag.24), check that the needle nearly touch the surface of the
tube holder without any contact. Turn the tube holder to its original position.
If the adjustment is correct, click on OK, the needle moves up.
If not: Click on OK, the needle moves up, modify the number of steps in the menu
System\Mechanical Systems(1)\Sampling needle adjustment(2) (Diag.22) Tube holder position
field. Increase number of steps if the needle is above the tube holder, decrease number of step if the
needle is too low inside the tube holder.
Click on enter then escape to validate the value.
Return in the menu sampling needle adjustment and rerun the control cycle (Diag.23).
Diag.24
Page 11/14

Tube holders door adjustment
After a complete tube holder assy adjustment the door may have to be adjusted.
Install the front panel (follow the procedure RAS306 Front panel dismantling).
Start the instrument.
CAUTION
Instrument running be carefull during intervention.
Pentra 60C+
Remove the tube holder and close the door smoothly, do not lock it, control where the door jams.
Remove the door (Diag.25):
Diag.25
Enter Menu\Mechanical systems\Sampling needle adjustment, click Id holder position button

and maintain the tube holder for few seconds closed, to lock it in this position.
Release the 2 screws (Diag.26-1) and adjust the door (Diag.26-2) according to the previous control:
1
Diag.26
Gently tight one of the screws, open the tube holder and fit the door.
Control again the door position, repeat until you get an acceptable adjustment of the door.
Page 12/14
RAS300B
From menu System\Mechanical Systems(1)\Sampling needle adjustment(2) you can setup
parameters for each sampling position (Diag.27):
Level: Sampling level (from the bottom
of each tube holders hole, in mm.).
1
2
Depth: Hole depth in mm. Standard

values depending on the tube holder.
Do not modify.
Id holder position: Returns in Position Id. field the tube holder position after
you close it.
Tube holder position: Number of steps

between the needle home position and the
top of the tube holder (about 400 steps).
Sampling positions are as follows (Diag.28):
Diag.27
3
Diag.28
See compatible tube list on next page.
Page 13/14
POSITION 1
P/N
ADDITIVE
CONDITIONS
rubber with groove

Hmogard
Hmogard
Hmogard
Hmogard
UltraSeal
rubber with groove
rubber with groove
rubber strongly not advisable
Hmogard
Hmogard
Hmogard
Hmogard
Hmogard
TYPE
OF CAP
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
with cap
PIERCIENG
*without cap
OBSERVATIONS
*Because of the cap thickness and the lack of space between the
holder and the top of the tube, the tube holder may not open correctly.
*The tube accepts a small sticker (not supplied by the manufacturer)

with cap
unlostable
unlostable
Man
Man
VOL. VACUUM STICKERS
MODEL
3ml
3ml
MAN.
K3-EDTA
5ml
Man+BC
5ml 2ml Man+BC
5ml 3ml Man+BC
5ml 3ml Man+BC
5ml 4,5ml Man+BC
5ml 3ml
Man
5ml 5ml
Man
5ml 3ml
Man
5ml 4ml Man+BC
5ml 2ml Man+BC
5ml 3ml Man+BC
5ml 4ml Man+BC
5ml 4,5ml Man+BC
5ml 4,5ml Man+BC
6385
VT-030STK K3-EDTA
*out of format without cap

** Microgard
with thread
0,5ml
0,5ml

**Cap fitted with an adaptator (other probe adjustment)
without cap
0,5ml
0,5ml
5ml 2,2ml
3ml
Becton D Vacutainer
368452 K3-EDTA
Becton D Vacutainer
367651 K3-EDTA
Becton D Vacutainer
367856 K3-EDTA
Becton D Vacutainer
367652 K3-EDTA
Becton D Vacutainer
367654 K3-EDTA
Terumo Venoject II VP-053SDK K3-EDTA
Terumo
Venoject VT-050STK K3-EDTA
Terumo
Venoject VT-053STK K3-EDTA
CML
ABX 3004002 TH5C0C K3-EDTA
Greiner
Vacuette
454087 K3-EDTA
Greiner
Vacuette
454086 K3-EDTA
Greiner
Vacuette
454036 K3-EDTA
Greiner
Vacuette
454223 K3-EDTA
LDM Paris
EDTAKE
POSITION 2
Venoject
Becton D Vacutainer
Terumo
POSITION 3
Comar R&D SystemsTX2B 18533 IF

POSITION 4
365973
365975
Sarstedt
901091
KABE
ABX 30010010777008RED
POSITION 5
Becton D Microtainer
POSITION 6
GBG 214 AS Standard tube holder positions: Position 1, position 2, position 3, position 4
GBG 215 AS Optional tube holder positions: Position 1, position 3, position 5, position 6
Page 14/14
Becton D Microtainer
Man: Manufacturer
BC: Barcode
Pentra 60C+
LMNE Balance adjustment

Procedure
CONCERNS
LMNE Balance calibration and forced calibration.
None
RAS303B
REQUIRED PRODUCTS
Fresh and normal blood samples (5 different samples at least)
INTERVENTION TIME
15 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Introduction
WARNING
WARNING
Pentra 60C+

Start the Analyzer.
Run a startup, click
button.
Calibration
Enter menu Technician Measurement(1)\LMNE Calibration(2) (Diag.1).
1
2
Diag.1
Page 2/4
RAS303B
Prepare a minimum of 5 different human blood samples without WBC alarm, insert one of the tube
and close the tube holder.
When the analysis cycle ends, the first result is displayed on the result chart table.
When the Green LED turns on, run another specimen, and so on until you get a minimum of 5
different results.
NOTE
Select results to be involved in the statistical calculation by clicking the checked box in Sel(1)
column.
To discard a result from the statistical calculation click the checked box again to remove the mark.
The auto-calculation module performs statistics on these results in order to obtain the best calibration
coefficient.
NOTE
The variation between the old and the new coefficient must be within
20% or the calibration would fail.
Click
(2) button to apply new calibration coefficient (Diag.2):
At least 5 results are necessary to calibrate the LMNE Balance.
Diag.2
Page 3/4

Confirm new coefficient by clicking OK(1) in the following box (Diag.3):
Pentra 60C+
Diag.3
New LMNE Balance coefficient is applied to the instrument.
Forced calibration
NOTE
LMNE Balance coefficient can be modified dircetly in the software
(Forced calibration). Enter System\Restricted tab and modify
LMNE Coef. value (1).
Diag.4
Page 4/4
Temperature adjustment
Procedure
CONCERNS
Heating coil and Thermostatic compartment temperature
check & Adjustment.
Thermometer
RAS305B
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS
Heating coil
Heating coil temperature check
Heating coil temperature adjustment
Thermostatic compartment
Thermostatic compartment temperature check
Thermostatic compartment temperature adjustment
WARNING
Pentra 60C+

Page 2/8
RAS305B
Heating coil
Heating coil temperature check
Concerns the control of the Heating coil temperature (reagent heating system).
Enter Menu: Service\Technician adjustment(1)\Heating coil adjustment(2) (Diag.1)
1
2
+ Reference CTN value
+ Run Cycle Number
Capture the CTN constant in this field.
Number of cycles to check the

temperature adjustment.
+ Consigne temperature
+ Run
Allows the modification of the

temperature instructions.
Starts the temperature adjustment check

cycle (The number of cycles to run depends
on the Run Cycle Number).
WARNING
This control must be made with the right side door closed and under
stabilised thermic conditions (5min at least after startup)
Diag.1
Page 3/8
Open the right side of the instrument and plunge the thermometer probe deeply in the LMNE chambers
inlet 1 (Diag.2):
IMPORTANT
Pentra 60C+
Make sure you have plunged

the thermometer probe deeply
Diag.2
into the liquid.
Close the right side door.

Enter in Run Cycle Number(1) (Diag.3) 5 cycles to run.
Diag.3
Click Run(2) button (Diag.3) and check that temperature is aroud 35C +/-1C every time you ear a
bip.
NOTE
Check is validated if you get during control at least 10 times the right
temperature. If an adjustment is required, when heating block has
been replaced for example, follow the procedure.
Page 4/8
RAS305B
Heating coil temperature adjustment
Check and eventualy change the CTN value.
heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.4
NOTE
The Heating coil CTN value is located on the CTN wire, close to the
main board, connected on J31 (Diag.4). Be sure to read the right
value, the sticker may be up or down.
Make a Heating coil temperature check (previous page of this procedure).
If you dont get the right temperature, adjust it by the mean of the Consigne temperature (Diag.1).
NOTE
Consigne temperature must be a rounded value 34, 35, 36...
When Consigne temperature increases, reagent preheating
temperature increases.
It may be necessary to restart the entire system after a Consigne
temperature changement.
Make a Heating coil temperature check as described at the begining of the procedure.
If required change the Consigne temperature value and check temperature again, until you get an
acceptable temperature.
+ A LED near the connector shows the
Page 5/8
Thermostatic copartment
Thermostatic compartment temperature check
Concerns the control of the Thermostatic compartment temperature.
Enter Menu: Service\Technician adjustment(1)\Thermostatic compartment adjustment(2) (Diag.5).
Pentra 60C+
Diag.5
+ Reference CTN value
+ Run Cycle Number
Capture the CTN constant in this field.
Number of cycles to check the

temperature adjustment.
+ Consigne temperature
+ Run
Allows the modification of the

temperature instructions.
Starts the temperature adjustment check

cycle (The number of cycles to run depends
on the Run Cycle Number).
WARNING
This control must be made with the right side door closed and under
stabilised thermic conditions (5min at least after startup).
Page 6/8
RAS305B
Open the right side of the instrument and put the thermometer probe in the CTN sensor (Diag.6)

Enter 5 cycles to run in Run Cycle Number(1) (Diag.7).
Diag.6
Diag.7
Click Run(2) button (Diag.7) and check that temperature is aroud 35C +/-1C every time you ear a
bip.
NOTE
Check is validated if you get during control at least 10 times the right
temperature. If an adjustment is required, when heating block has
been replaced for example, follow the procedure.
Page 7/8
Thermostatic compartment temperature adjustment
Check and eventualy change the CTN value, thermostated compartment CTN value is located on the
CTN wire, close to the main board, connected on J33 (Diag.8).
Pentra 60C+
A LED near the connector shows the

heating command:
- when lit off, heating is off
- when blinking, heating is on
- when lit on, heating is maximum
Diag.8
NOTE
The Thermostatic compartment CTN value is located on the CTN
wire, close to the main board, connected on J33 (Diag.8)
Be sure to read the right value, the sticker may be up or down.
Make a Thermostated compartment temperature check (previous page of this procedure).
If you dont get the right temperature, adjust it by the mean of the Consigne temperature (Diag.7).
NOTE
Consigne temperature must be a rounded value 34, 35, 36...
When Consigne temperature increases, Thermostatic
compartment temperature increases.
It may be necessary to restart the entire system after a Consigne
temperature changement.
Make a Thermostated compartment temperature check as described at the begining of the procedure.
If required change the Consigne temperature value and check temperature again, until you get an
acceptable temperature.
Page 8/8
Front panel dismantling

Procedure
CONCERNS
Hexagonal key size 3

Flat screw driver
Torx screwdriver N10
RAS306B
REQUIRED PRODUCTS
None
INTERVENTION TIME
10 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Front panel
Dismantling
Mounting
WARNING
Pentra 60C+

Page 2/4
RAS306B
Front panel
Dismantling
Switch off the instrument.
Disconnect power supply cable.
Open the reagent door and remove the 4 bottles (disconnect tubes from the stoppers).
Open the pneumatic access door (Right side of the instrument).
Open the left side panel and the main board door (lock it).
A flat cable is connected at the back of the main board, becarefull to

not disconnect it, nor damage it, when you open the door.
Remove the cover.
Make sure the probe is lift and push the carriage back.
Unscrew the K30x16 torx screw (several turns) (Diag.1&2).
Unscrew the 2 CHC M4x6 screws (Diag.1-1):
Diag.1
WARNING
Diag.2
Page 3/4

Unscrew the 4 CHC M4x10 screws in the reagent compartment (Diag.3):
Pentra 60C+
Diag.3
Lift and pull a little the front panel, to free space in order to disconnect the sampling LEDs command
in the internal side of the panel (Diag.4):
Diag.4
Mounting
Reassemble following Dismantling procedure in reverse order.
WARNING
Beware to center the front panel and the tube holders door as
precisly as possible. Becarefull not to clamp one of the reagents tube
behind the reagent compartment.
Start the Analyzer.
Run an Autocontrol cycle to check that all is operating normally and especially the tube holders door.
Page 4/4
6 month maintenance
Procedure
CONCERNS
Hexagonal keys
Dynamometric screw driver A302, A301, A300
Cutting pliers
Flat screw driver
Philips screw driver
Torx keys
RAS307B
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY
See Maintenance table

6 month spare parts maintenance kit: XEA710BS
Pentra 60C+
REQUIRED TOOLS
6 month maintenance
6 month maintenance
Rinsing block
Reagent syringe
Draining & counting syringes
Instrument cleaning
WARNING
Pentra 60C+

KIT XEA710BS
P/N
DESIGNATION
FAA013A
FAA055A
FAA057A
FAA065A
XDA621A
XDA622A
XEA019A
QTY
Oring 1.80x1.20 Nitrile (Rinsing block)

Oring 1.07x1.27 Fluorocarbon (Rinsing block)
Oring 5x1 Nitrile (Rinsing block)
Oring 6.30x2.40 Silicon (Reagent syringe)
Oring 30.80x3.60 + Washer (Vacuum/Waste pump)
Oring 15.54x2.62 + Washer (Reagent syringe)
Silicon grease
1
1
1
1
2
4
1
WARNING
If the instrument is suspected not to be perfectly clean, perform a
Sampling probe must be in perfect condition in order to get correct
MDSS functions.
Page 2/9
RAS307B
6 month maintenance
Rinsing block: Oring replacement (1x FAA013A, 1x FAA055A & 1x FAA057A)
Switch off the instrument and disconnect power supply cable.
Open the right side door.
Move the carriage to access to the rinsing block.
Remove the 2 rinsing block screws (Diag.1).
Lift the locker (Diag.2) to free the probe and remove the rinsing block.
Diag.2
Replace all the Orings from the rinsing block (use a small amount of silicon grease to lubricate FAA057A
Oring):
FAA055A
FAA013A
FAA057A
6 month maintenance
Diag.1
Diag.3
Reassemble in reverse order.
IMPORTANT
Do not tight too much the rinsing block screws.
Page 3/9
6 month maintenance
Reagent syringe: Oring replacement (1x FAA065A & 4x XDA622A)
Drain manually all the syringes before starting operations.
Remove the left side panel and open the main board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when you
open the door not to disconnect nor to damage it.
Disconnect the following tubes (Diag.4):
Pentra 60C+
Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3.
1
Diag.4
Unscrew the 2 fixation screws and remove carefully the reagents syringe (1).
Push several times the pistons above the waste container to drain the syringe.
WARNING
A washer is adapted to each Oring joint thickness, keep the Oring and the washer together.
Lyse piston Oring (small one) had no washer.
On a piece of absorbant paper open the reagents syringe (9x CHC M3x12, 2x FX M3x12).
Replace all Orings and washers by new one from the 6 month maintenance kit (use one drop of silicon
grease in between 2 fingers to lubricate the Orings) (Diag.5):
TIGHTENING TORQUES
9x CHC M3x12:
400mN.m (56.8 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
Diag.5
Reassemble in reverse order (Use a dynamometric screw driver to tighten the syringe).
Page 4/9
RAS307B
1- Oring replacement on Draining Syringe (1x XDA621A)
Remove the back side panel (6x CHC M4x6) (Diag.6).
Disconnect electrical connectors behind the instrument (Diag.7).
Diag.7
6 month maintenance
Diag.6
Diag.8
Cut the tyraps (Diag.8).

Disconnect grounding wire from the frame (Diag.8).
Put a piece of aborbant paper under the syringe and disconnect both tube.
Page 5/9
6 month maintenance
Diag.10
Pentra 60C+
Diag.9
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.9).
Remove the syringe.
Drain the syringe pushing the piston nut up and down several times.
According to the type of syringe fitted, unscrew the 4x CHC M4x16 screws (Diag.10) or unscrew the
syringe body and open it (Diag.11).
Diag.11
Diag.12
Replace the Oring (Diag.13) by the new one from the 6 month maintenance kit (use one drop of silicon
grease in between 2 fingers to lubricate Oring and piston).
Reassemble in reverse order according to diagram 12 (Molded syringe body Diag.11 has to be rotated
until the mark on the body is located between the two marks of the motor Diag.14).
Anti-extrusion
ring
Oring
Oring thickness
wedge
Diag.13
Diag.14
Page 6/9
RAS307B
2- Oring replacement on Counting Syringe (1xXDA 621 A)
Remove the left side panel and open the mian board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when

you open the door not to disconnect or damage it.
Remove the back side panel.
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block).
Diag.15
Diag.16

Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe
(Diag.17).
6 month maintenance
Disconnect the electrical connectors behind the instrument (Diag.15).
Diag.18
Diag.17
Page 7/9
6 month maintenance
Remove the syringe.
According to the type of syringe fitted (Diag.20&21), unscrew the 4x CHC M4x16 screws (Diag.18) or
unscrew the syringe body and open it.
Pentra 60C+
Diag.19
Replace the old Oring joint and washer by new one from the 6 month maintenance kit (use one drop
of silicon grease in between 2 fingers to lubricate the Oring) (Diag.19).
Diag.21
Diag.20
Reassemble in reverse order according to diagram 22 (Molded syringe body Diag.20 has to be rotated
until the mark on the body is located between the two marks of the motor Diag.23).
Anti-extrusion
ring
Oring
Oring thickness
wedge
Diag.22
Diag.23
Page 8/9
RAS307B
Instrument cleaning
Dilute the 12cl bleach to 1 part of bleach for 4 of deionize water (1/5).
Instrument environment must be cleaned.
No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins,
have to be used. For small or sensitive assemblies use accurate drier papers.
All assemblies suspected to have been in contact with biohazardous material must be disinfected with
diluted bleach (the stainless steel must be bleached below 30Celsius).
Blood stains or salt marks must be cleaned with spray detergent first.
Clean the filter under the rinse chamber (Diag.24) from the pieces of cap rubber:
Diag.24
Reinstall all the assemblies and setup the instrument back to its initial configuration.
6 month maintenance
Concerned assemblies:
All the thermostated compartment
Outer surfaces of the instrument (perpex, covers, reagent locations....)
Waste connector plug
Liquid valve push button
Assemblies close to the needle
Tube holder assembly
Overflow trays
Page 9/9
Yearly maintenance
Procedure
CONCERNS
Hexagonal keys
Cutting pliers
Flat screw driver
Torx keys
RAS308B
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY

Yearly spare parts maintenance kit: XEA486BS
Pentra 60C+
REQUIRED TOOLS
Yearly maintenance
Yearly maintenance
Sampling needle
Sampling syringe
Reagent syringe
WARNING
Pentra 60C+
KIT XEA486BS
P/N
DESIGNATION
Page 2/13
DAJ007A
FAA040A
FAA046A
FAA064A
FAA066A
FAA067A
GBG275A
JAJ007A
XEA286AS
XEA663A
9.5V 20W lamp

Oring 2.75x1.60 Viton
Flat EPO joint 0.5
Polyethylene tape
Waste chamber Joint + Washer
Coaxial kit LMNE flowcell
QTY
1
1
2
2
4
4
4
1m
1
1
WARNING
RAS308B
Yearly maintenance
Sampling needle: Needle XDA655DS
Pull carriage to access to the probe.
TORQUE
Diag.2
Rinsing block screws:

120mN.m (17 Ozf.in)
Yearly maintenance
Diag.1
Replace the needle and tight the rinsing block.
Page 3/13
Yearly maintenance
Sampling syringe: Oring replacement (2x FAA064A)
Pentra 60C+
Switch on the instrument

Run Menu: Maintenance\Technician\Others\Maintenance carriage position
Open the pneumatic access door (Right side of the instrument)
Disconnect the diluent tube between valve 18 and sampling syringe (Diag.3) The diluent is drained
through the sampling syringe
Unscrew both fixation screws of the Sampling syringe on the carriage (Diag.4)
Diag.3
Diag.4
Disconnect from the syringe output tube (Diag.5)

Unscrew the 3 FX M3x12 screws from the bottom of the syringe (Diag.6)
Diag.5
Diag.6
Remove the bottom plate (3x FX M3x12), the piston axle and the 2 Oring joints
Replace both Oring joints by new one in the yearly maintenance kit (use one drop of silicon grease
between 2 fingers for both Oring)
Reassemble following previous steps backward
When startup and prime are done check that there is no leak
TORQUE
3x FX M3x12:
400mN.m (56.8 Ozf.in)
Page 4/13
RAS308B
5DIFF syringe: Oring replacement (1x FAA040A; 4x FAA067A)
Open the left side panel and the mother board door (lock it).
WARNING
A flat cable is connected at the back of the mother board, becareful

when you open the door that you dont disconnect nor damage it.
Disconnect the tubes (Diag.7):
Valve 2 inlet 2,
Valve 3 inlet 3,
Valve 5 inlet 1
Unscrew both fixation screws of the 5DIFF syringe (Diag.8)
Disconnect gently from the syringe the 2 left tubes.
Diag.8
Remove the bottom plate (2x FX M3x12, 4x CHC M3x12), the piston axles and the Oring joints (Diag.9):
Diag.9
Yearly maintenance
Diag.7
Diag.10
TORQUES
Replace all Oring joints by new one in the yearly maintenance kit (use
one drop of silicon grease between 2 fingers for each Oring (Diag.10).
Reassemble following previous steps backward.
When startup is done check that there is no leak.
4x CHC M3x12:
400mN.m (56.8 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
Page 5/13
Yearly maintenance
Optical bench: Lamp replacement (1x DAJ007A)
Pentra 60C+

Open the cover.
Disconnect the lamp supply (Diag.12).
Unscrew lamp fixation screws (few turns) (Diag.11).
Diag.11
Diag.12
Turn the lamp and remove it (Diag.12).
Change the lamp.
Put back the fixation system and block the screws.
Reconnect the lamp supply.
WARNING
Do not touch lamp bulbe with the fingers, use a piece of cloth or paper
to manipulate the lamp.
Page 6/13
RAS308B
LMNE Flowcell: 1- Coaxial replacement (1x XEA663A)
Open the cover
Open the left side panel and the mother board door (block it)
WARNING
when you open the door that you dont disconnect nor damage it.
Diag.13
Disconnect the T connector (few diluent drops will leak) (Diag.14&15)
Yearly maintenance
Open the optical bench cover (4 screws Diag.13)
Diag.14
Diag.15
Page 7/13
Yearly maintenance
To access ground fitting under the flowcell, unscrew the 2 fixation srews (Diag.16)
Pentra 60C+
Diag.16
Turn slowly the flowcell (take care to the washers)

Unscrew the ground screw using a small philips screwdriver (Diag.17)
Diag.17
Put the new LMNE coaxial instead

Reassemble following previous steps backward (except optical bench cover)
Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of the air bubbles stuck
to the inner optical surfaces
Check that there is no leak and that flowcell contains no or just a very few air bubbles
Put back the optical bench cover
Page 8/13
RAS308B
2- LMNE Flowcell adjustment check
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3)
(Diag.18).
Mix RBC/PLT Latex thoroughly, close tube holder to start sampling.
When optical bench is well adjusted:
1
2
Diag.18
Check that the displayed values are within the range.

Target values:
PARAMETER
LMNE LAMP
TRANSFER TIME
RESISTIVE CHANNEL
ABSORBANCE CHANNEL
TARGET VALUE
6.00
200
50
180
RANGE
5.50 to 6.50
150 to 250
45 to 55
180 10
Yearly maintenance
If you are out of range perform a RAS311 LMNE Flowcell adjustment procedure.
Page 9/13
Yearly maintenance
Diluent reservoir: Joint & Washer replacement (1x XEA286AS)
Pentra 60C+
Switch on the instrument.

Run Menu: System\Hydraulics Systems\Drain chambers\Diluent reservoir.
When cycle is finished disconnect the drain tube under the reservoir.
Unscrew the 2 fixation screws under diluent reservoir (few turns to release diluent reservoir support)
(Diag.19):
Diag.19
Lift reservoir, take care that no tube is diconnected from reservoir.

Unscrew the 4 reservoir screws on top.
Replace joint and washer by new one in the yearly maintenance kit.
Locate new washer and joint against the reservoir top, then the diluent reservoir body (Diag.20):
NOTE
Locate reservoir body to have
inferior support turned back
and tubes in the upper part in
front.
Diag.20
Page 10/13
Screw the 4 reservoir screws on top.

Put back diluent reservoir in its location and block screws.
Connect drain tube under diluent reservoir.
TORQUE
4x CHC M3x20:
120mN.m (17 Ozf.in)
RAS308B
Draining chamber: Oring replacement (1x FAA066A)
Run Menu: Service\Hydraulics Systems\Drain chambers\All.
Turn gently the chambers bottom between 2 fingers (Diag.21):
Replace the Oring joint (Diag.22):
Diag.22
Repeat with the other chambers (Diag.23):
Yearly maintenance
Diag.21
Diag.23
Page 11/13
Yearly maintenance
Counting Heads: 1- RBC/WBC Coaxial cable Oring replacement (2x FAA046A)
Pentra 60C+

Run Menu: Systems\Hydraulics Systems\Drain Chambers\All.
Put a piece of white paper or cloth under the Counting chamber assembly.
Record the tube positions before dismantling the Counting chamber assembly.
Disconnect the chamber tubes (not the wastes).
Dismantle the Counting chamber assembly, 3 screws (Diag.24):
Diag.24
Dismantle the electrode loosening the 2 fixation screws (Diag.25):
Diag.25
Diag.26
Use a previously cut micropipette tip to replace the electrode Oring joint as shown in
diagram 26.
Reassemble electrode.
Repeat with the other electrode (Diag.27).
Page 12/13
RAS308B
2- Counting head aperture joint replacement (4x GBG275A)
Carefully dismantle the counting head (Diag.27) and plunge the aperture in distilled water.
Replace both EPO joints by new one (Diag.28).
Clean the chamber and the counting head with liquid soap, do not introduce any sharp instrument
inside so as to avoid damaging the inside of the chamber and the aperture.
TORQUE
2x CHC M3x12:
120mN.m (17 Ozf.in)
Diag.28
CAUTION
Do not manipulate the aperture using hard instruments. Clean the
aperture with a piece of soft paper or preferably, in between 2 fingers.
Rinse thoroughly with distilled water.
Dry the exterior of the chamber with a soft paper.
CAUTION
Do not apply too much pressure on the electrode fixation screws, as it
can break the aperture (tightening torque = 120mN.m / 17 Ozf.in).
Yearly maintenance
Diag.27
It is recommended to reconnect the tubes on the counting head before

reassembling the "electrode/chamber/counting head" assy in order
to avoid applying constraint on the chamber.
Repeat operations for the other counting head.

Reassemble electrode.
Repeat with the other electrode (Diag.27).
Page 13/13
Every 2 years maintenance

Procedure
CONCERNS
Hexagonal keys
Cutting pliers
Flat screw driver
Torx keys
RAS309B
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY

Every 2 years spare parts maintenance kit: XEA630CS
Pentra 60C+
REQUIRED TOOLS

Sampling carriage
Reagents syringe
Pentra 60C+
KIT XEA630CS
P/N
DESIGNATION
GBC030A
GBC031A
GBG169A
GBG260A
GBG274A
GBG288A
KAA009A

Sampling carriage percutor
31mm piston spacer
31mm piston
Oring tightener
CHC M3 screw
QTY
4
1
1
2
2
1
6
WARNING
WARNING
Page 2/8
RAS309B
Sampling carriage: Percutor, Oring tightener replacement (1x GBG169A, 1x GBG288A)
Pull carriage to access to the probe.
Diag.1
Diag.2
Replace the probe, the percutor and the rinsing block part (Diag.3).
GBG170A
GBG169A
Diag.3
IMPORTANT
Use new Orings when performing 6 Month maintenance at the same time.
TORQUE
Rinsing block screws:

120mN.m (17 Ozf.in)
Page 3/8

Reagents syringe: Pistons (1x GBC031A & 4x GBC030A)
Remove the left side panel and open the main board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when you
open the door not to disconnect nor damage it.
Disconnect the following tubes (Diag.4):
Pentra 60C+
Valve 6 inlet 3,
Valve 7 inlet 3,
Valve 8 inlet 3,
Valve 9 inlet 3,
Valve 11 inlet 3.
1
Diag.4
Unscrew the 2 fixation screws and remove carefully the reagents syringe (1).
Push several times the pistons above the waste container to drain the syringe.
On a piece of absorbant paper open the reagents syringe (9x CHC M3x12, 2x FX M3x12).
Replace the pistons.
IMPORTANT
Replace at the same time the Orings and washers from the 6 month
maintenance kit and according to the RAS307 procedure.
TIGHTENING TORQUES
9x CHC M3x12:
400mN.m (56.8 Ozf.in)
2x FX M3x12:
400mN.m (56.8 Ozf.in)
Diag.5
Reassemble in reverse order (Use a dynamometric screw driver to tighten the syringe).
Page 4/8
RAS309B
1- Draining Syringes Piston replacement ((1x GBG274A + 1x GBG260A) or 1x GBG052A)
Remove the back side panel (6x CHC M4x6) (Diag.6).
Disconnect electrical connectors behind the instrument (Diag.7).
Diag.7
Diag.6
Diag.8
Cut the tyraps (Diag.8).

Put a piece of aborbant paper under the syringe and disconnect both tube.
Page 5/8
Diag.10
Pentra 60C+
Diag.9
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.9).
Remove the syringe.
According to the type of syringe fitted, unscrew the 4x CHC M4x16 screws (Diag.10) or unscrew the
syringe body and open it (Diag.11).
Diag.11
Diag.12
Replace the piston according to the syringe model:

- Diag.11 replace piston without crosspiece GBG260A (new molded syringe)
- Diag.12 replace piston with crosspiece GBG260A (old machined syringe)
IMPORTANT
Use new Orings when performing 6 Month maintenance at the same time.
Reassemble in reverse order (Molded syringe body Diag.11 has to be rotated until the mark on the body
is located between the two marks of the motor Diag.13).
Diag.13
Page 6/8
RAS309B
2- Counting Syringes Piston replacement ((1x GBG274A + 1x GBG260A) or 1x
GBG052A)
Proceed the same maner as for Draining syringe except for the following points:
Disconnect the electrical connectors behind the instrument (Diag.14).
Diag.15

Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe
(Diag.16):
Diag.16
Diag.14
Page 7/8

Pentra 60C+
Page 8/8
Decontamination & Rinse

Procedure
CONCERNS
Instrument decontamination before maintenance operation in the
following cases:
- Instrument removed from a biohazardous area,
- Maintenance intervention on contaminated assemblies.
and instrument rinse
Hexagonal keys
Clamps
Flat screw driver
Torx keys
RAS310B
REQUIRED PRODUCTS
Fungicidal, bactericidal, virus killing detergent spray, non
corrosive for metals, Non plastic altering.
Bleach solution 12Cl / Deionize water / Protection gloves /
Absorbant paper / Distilled water
INTERVENTION TIME
1h30min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Decontamination
Preparation
Manual decontamination
Analysis circuit decontamination
Drain & Rinse
WARNING
Pentra 60C+

Page 2/6
RAS310B
Decontamination
Preparation
Switch on the instrument
Open the pneumatic access door (Right side of the instrument)
Run Menu: System\Hydraulics Systems(1)\Cleaning Cycles(2)\Concentrated cleaning(3) (Diag.1):
Diag.1
Confirm by clicking Ok, a rinse cycle is started.
Wait until the instrument displays the following screen (Diag.2):
Diag.2
Pour 3ml of Minoclair in each chamber (Diag.3) and click Ok to continue (Diag.2).
Diag.3
When cycle is finished, switch off the instrument and remove the supplying cable
Open the instrument cover
Spray the bactericidal cleaner on all biohazardous areas and wait for 10 minutes (assemblies in contact with the biohazardous material such as instrument cover, tube holder, keyboard, start key, assemblies
close to the SN...
Page 3/6

Manual decontamination
Dilute the 12cl bleach to 1 part of bleach for 4 of deionize water (1/5).
Instrument environment must be cleaned and decontaminated.
No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins,
have to be used. For small or sensitive assemblies use accurate drier papers.
All assemblies suspected to have been in contact with biohazardous material must be disinfected with
diluted bleach (the stainless steel must be bleached below 30Celsius).
Blood stains or salt marks must be cleaned with spray detergent first.
Pentra 60C+
Concerned assemblies:
Outer surfaces of the instrument (perpex, covers, reagent locations....)
Waste connector plug
Liquid valve push button
Assemblies close to the needle
Tube holder assembly
Overflow trays
Page 4/6
Reinstall all the assemblies and setup the instrument back to its initial configuration.
RAS310B
Analysis circuit decontamination
Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts of deionize water (1/
10).
Prepair 1 bottle containing 1/2 litre of distilled water.
Replace the reagent bottles by the diluted bleach bottle.
Run 6 All reagents prime cycles Menu: Systems\Hydraulic Systems(1)\Prime cycles(2)\All
Reagents(3) (Diag.4).
2
3
Diag.4
Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5)
Run 15 burn-in cycles(2) Menu: Systems\Burn in cycle(1)\Run burn in(3) (Diag.5). leave the instrument operating until it stops
1
2
3
Diag.5
Page 5/6

Drain & Rinse
Remove the 3 reagent straws from the bottle containing the diluted bleach.
Wrap the straws in absorbant paper.
Reagents(3) (Diag.4): the bleach is drained.
Replace the diluted bleach by the distilled water bottle and re-plunge the straws in distilled water
Reagents(3) (Diag.4) (Rinse).
Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbant paper).
Reagents(3) (Diag.4): the distilled water is drained.
Run a STAND BY cycle.
Pentra 60C+
Re-install the reagent bottles and the straws.
Page 6/6

Close the instrument cover.
WARNING
When rinse is finished check that LMNE chamber is empty.
LMNE Flowcell adjustment

Procedure
CONCERNS
Instrument adjustment:
LMNE Flowcell
Hexagonal keys
Flat screw driver
Torx keys
RAS311B
REQUIRED PRODUCTS
Latex RBC\Plt
Fresh blood sample
Calibrator
INTERVENTION TIME
1h
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

LMNE Flowcell
Preparation
LMNE Flowcell adjustment check
LMNE Flowcell position adjustment
WARNING
Pentra 60C+

Page 2/6
RAS311B
LMNE Flowcell
Preparation
This adjustment procedure must be carried out when LMNE flowcell is moved or replaced, or when
LMNE tube is changed. No adjustment is required when LMNE Lamp is replaced.
WARNING
Never dismantle Emission gun or even unlock it. The adjustment is
factory made and cannot be performed in the field.
Open the cover.
Open the left side panel and the main board door (block it).
WARNING
when you open the door that you dont disconnect nor damage it
Diag.1
Run Menu: Systems\Hydraulics systems\Rinse\Rinse cytometer to get rid of air bubbles stuck to
the inner optical surfaces.
Check that the flowcell contains no or just a very few air bubbles.
Menu: Maintenance\Technician\Measurement\LMNE Adjustment allows the adjustment of the

entire optical bench parameters. A 1:400th dilution of the RBC/PLT Latex is prepared (5l of Latex, 2ml
of diluent) in the LMNE chamber. This dilution is then transfered toward the optical chamber and injected
The measurments are continuously displayed every 700ms during a total of 27 seconds.
Page 3/6

LMNE Flowcell adjustment check
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3) (Diag.2).
When optical bench is well adjusted:
1
2
Pentra 60C+
Diag.2
Check that the displayed values are within the range.

Target values:
PARAMETER
LMNE LAMP
TRANSFER TIME
RESISTIVE CHANNEL
ABSORBANCE CHANNEL
TARGET VALUE
6.00
200
50
180
If you are out of range or to get a better adjustment follow the procedure.
Page 4/6
RANGE
5.50 to 6.50
150 to 250
45 to 55
180 10
RAS311B
LMNE Flowcell position adjustment
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3) (Diag.2).
Adjust LMNE lamp voltage around 6V: Adjust by R11 potentiometer located on optical bench board
(Diag.3).
NOTE
with a voltmeter.
Diag.3
Adjust Resistive channel to 50: Adjustment is made by R136 potentiometer located on mother board
(Diag.6 on next page).
Adjust Absorbance channel to the maximum (and above 170): Adjustment is made by lateral or axial
reception gun movement, use toothed wheel (Diag.4) and lateral screw (Diag.5).
Diag.4
Test points on Optical bench board, near R11 potentiometer, can be used to check 6V
Diag.5
NOTE
If you have difficulties to adjust Absorbance, you can increase gain with the R148
potentiometer (Diag.6 on next page). Once you get the highest absorbance adjustment,
decrease slowly gain (R148) to get a correct optical gain adjustment with normal fresh
blood.
Page 5/6

Check that Transfer time is in limits of range (around 200): Transfer time is not adjustable and must be
between 150 and 250.
NOTE
Transfer time bargraph is used to ensure the correct Right/Left location of the light
rectangle in the flowcell. When the optimal value is reached the transfer time is around
200 because of the constant height between guns alignment and flowcell.
This height is guaranteed by the washers under the flowcell, always put them back
even when the flowcell is replaced by a new one.
Pentra 60C+
R148
R136
Diag.7
Put back the Optical Bench cover
NOTE
After intervention check, with normal fresh blood, that you have a correct matrix.
Page 6/6
Chambers adjustment
Procedure
CONCERNS
Chambers assembly adjustment
Hexagonal keys
Dynamometric screw river A300, A301, A302
Flat screw driver
Torx keys
RAS312B
REQUIRED PRODUCTS
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS
Chambers adjustment
Chambers adjustment
Alignment check
Alignment adjustment
WARNING
Pentra 60C+

Page 2/4
NOTE
It is mandatory to perform the Probe adjustment procedure after this
adjustment.
WARNING
Needle extrimity is teflon coated and is a very fragile part.
Beware not touch any part with the needle extremity during
adjustments.
RAS312B
Chambers adjustment
Alignment check
This adjustment procedure must be carried out when chamber assembly is moved or replaced.
Open the cover and the right access door.
Locate probe as shown in diagrams 1 & 2:
Diag.2
Push the carriage backward (without lifting the probe), check that there is the same height between
probe and first and last tops of chamber (Diag.3 & 4):
Chambers adjustment
Diag.1
Diag.3
Diag.4
If you need to get a better adjustment follow this procedure.
Page 3/4
Chambers adjustment
Alignment adjustment
Free the chamber support by loosening the 5 screws (Diag.5)
WARNING
Pentra 60C+
Diag.5
Do not touch the chamber with the needle

extremity or the needle will be damaged.
Locate probe as shown in diagrams 6 & 7:
Diag.6
Diag.7
Tighten gently the left upper screw.
Push the carriage backward (without lifting the probe), check that there is the same height between
probe and last top of chamber (Diag.8 & 9)
Diag.8
If necessary adjust position moving up and down chamber
Perform an alignment check as described at the begenning of this procedure
When adjustment is correct, tighten screws and perform a Probe Adjustment procedure
Page 4/4
Diag.9
Main board adjustment

Procedure
CONCERNS
Hexagonal keys
Small Flat screw driver
Voltmeter
RAS313B
REQUIRED PRODUCTS
INTERVENTION TIME
30min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS
Pentra 60C+

Hgb blank adjustment
RBC\Plt gain adjustment
WBC\Baso gain adjustment
Drain sensor adjustment
Transfer sensor adjustment
Motor current adjustment
Threshold adjustment
Page 2/9
WARNING
RAS313B
Hgb blank adjustment
Open the cover and the left access door to main board.
Run Menu: Service\Technician Measurment\Gain adjustment\Hgb Blank Adjustment (Diag.1).
The utility carries out a rinsing cycle of the WBC chamber and continuously displays the voltage used
by the converter. The adjustment cycle lasts for 20 seconds.
The adjustment is carried out with the R248 potentiometer (Diag.2).
The final adjustment voltage is approximately 4.7 Volts +/- 0.05V.
Once adjustment is done, check correct voltage between TP1 and ground.
Diag.1
Diag.2
Page 3/9

RBC\Plt gain adjustment
Open the cover and the left access door to main board
A special cycle allows the dilution of the RBC/Plt LATEX to a 1/220th (10 l of LATEX with 2.2 ml of
diluent). A special count programme carries out the calculations of the mean volume in the predefined
zones and displays them every 700 ms. The duration of the measurement cycle is 21 seconds
The adjustments are made using R133 potentiometer for the RBC and R135 for the Plt (Diag.4)
Run Menu: Service\Technician Measurment\Gain adjustment\RBC/Plt Gain (Diag.3)
Two graphs allow a visual check of the adjustments (Diag.3)
Target Values:
RBC=78 / Plt=112
IMPORTANT
Pentra 60C+
Mix RBC/PLT Latex thoroughly before sampling.
Diag.3
Diag.4
Page 4/9
RAS313B
WBC\Baso gain adjustment
A special cycle allows the dilution of the RBC/Plt LATEX to a 1/733rd (3L of LATEX with 2.2 ml of
diluent). A special count programe carries out the calculations of the mean volume in the predefined
zones and displays them every 700 ms. The duration of the measurement cycle is 21 seconds
The adjustments are made with the aid of a R134 potentiometer (Diag.6)
Run Menu: Service\Technician Measurment\Gain adjustment\WBC/BASO Gain (Diag.5)
A graph allows a visual assessment of the adjustments (Diag.5)
Target Value:
WBC/Baso=102
IMPORTANT
Diag.5
Mix RBC/PLT Latex thoroughly before sampling.
Diag.6
Page 5/9

Drain sensor adjustment
Pentra 60C+
Open the cover and the left access door to main board.
Run Menu: Service\Technician Adjustment\Draining sensor adjustment\Run (Diag.7).
The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test point TP52,
R287 potentiometer on the mother card Diag.8).
The utility then fills the sensor with the diluent. check that the voltage on TP52 is less than 1 Volt.
Diag.7
Diag.8
Page 6/9
RAS313B
Transfer sensor adjustment
Run Menu: Service\Technician Adjustment\Transfer sensor adjustment\Run (Diag.9)
The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test point TP48,
R286 potentiometer on the mother card Diag.10)
The utility then fills the sensor with the diluent. check that the voltage on TP48 is less than 1 Volt.
Diag.9
Diag.10
Page 7/9

Motor current adjustment
Check and adjust, when necessary, all following voltages:
MOTORS
Pentra 60C+
Draining syringe
Counting syringe
Dilutor syringe
Optical Bench
Injector syringe
Horizontal carriage
Sample syringe
Probe carriage
VOLTAGE
TP5
TP6
TP7
TP8
4.8 V + 0.05 V
4.8 V + 0.05 V
4 V + 0.05 V
3 V + 0.05 V
POTENTIOMETER
R149
R150
R151
R152
TP10
TP11
TP12
3 V + 0.05 V
2 V + 0.05 V
4.5 V + 0.05 V
R154
R155
R156
Potentiometers and Test points are located on Main board as shown on the next diagram:
Draining syringe
Counting syringe
Reagents syringe
Optical Bench
Injector syringe
Horizontal carriage
Sample syringe
Probe carriage
Up/Down
Page 8/9
TEST POINTS
RAS313B
Threshold adjustment
Check and adjust, when necessary, all following voltages:
THRESHOLDS
BASO
RBC
PLT
LMNE CIS
LMNE OD
TEST POINTS
VOLTAGE
TP14
TP13
TP2
TP3
TP4
300 mV + 5
300 mV + 5
300 mV + 5
650 mV + 5
350 mV + 5
POTENTIOMETER
R157
R158
R159
R160
R161
Potentiometers and Test points are located on Main board as shown on the next diagram:
Diag.18
PLT
LMNE OD
LMNE CIS
RBC
BASO
Page 9/9
Vacuum adjustment
Procedure
CONCERNS
Concerns counting and draining syringe vacuum control and
adjustment.
Barflex
RAS314B
REQUIRED PRODUCTS
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS
Vacuum adjustment
Vacuum adjustment
Draining syringe vacuum check
Counting syringe vacuum check & adjustment
WARNING
Pentra 60C+

Page 2/5
RAS314B
Vacuum adjustment
Draining syringe vacuum check (No adjustment available)
Disconnect the tube on the draining syringe and replace it by the Barflex tube (Diag.1):
Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Draining syringe(3) (Diag.2):
1
2
3
Vacuum adjustment
Diag.1
Diag.2
Diag.3
Check vacuum on the Barflex, vacuum must be around 260mb and must be stable. Press Ok
(Diag.3).
Disconnect the Barflex and connect back the tube on the draining syringe.
Page 3/5
Vacuum adjustment
Counting syringe vacuum check
WARNING
Open the left side panel and the mother board door (lock it)

when you open the door that you dont disconnect or damage it
Pentra 60C+
Disconnect the lowest tube on the

counting syringe and replace it by the
Barflex tube (Diag.4):
Diag.4
Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Counting syringe(3)
(Diag.5):
1
2
3
Diag.5
Check vacuum on the Barflex while the following screen is displayed (Diag.6), vacuum must be at
220mb.
Press Ok (Diag.6).
Disconnect the Barflex and connect back the tube on the counting syringe.
Diag.6
Page 4/5
RAS314B
Counting syringe vacuum adjustment
Open the left side panel and the mother board door (lock it)
WARNING
when you open the door that you dont disconnect or damage it
Disconnect the lowest tube on the
counting syringe and replace it by the
Barflex tube (Diag.7):
Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Vacuum value(3) (Diag.8):
1
3
Vacuum adjustment
Diag.7
Diag.8
Check vacuum on the Barflex while the following screen is displayed, vacuum must be around 220mb.
Press Ok (Diag.6).
Change the step value. If vacuum is too low increase step value.
Repeat vacuum check and step value adjustment until you get the correct vacuum.
Disconnect the Barflex and connect back the tube on the counting syringe.
Page 5/5
Bubbling adjustment
Procedure
CONCERNS
Bubbling adjustment.
None
RAS315B
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS
Bubbling adjustment
Bubbling adjustment
Bubbling adjustment
WARNING
Pentra 60C+

Page 2/3
RAS315B
Bubbling adjustment
Bubbling adjustment
Bubbling is factory adjusted and normally does not required further adjustment.
If an adjustment is necessary follow this procedure.
Enter Menu:Service\Technician Adjustment(1)\Bubbling(2) (Diag.1):
1
2
Diag.1
Enter new step value for each bubbling(3) (Diag.1) and click Accept(4) (Diag.1).
NOTE
When number of steps rises, bubbling is increasing.
BUBBLING
FIRST DILUTION
LMNE
WBC/BASO
RBC/Plt
Hgb
LOW
LIMIT
100
100
100
100
300
NORMAL
300
300
300
300
400
HIGH
LIMIT
Bubbling adjustment
400
400
400
400
500
Page 3/3
Step by step analysis cycle

Procedure
CONCERNS
Instrument control:
Analysis cycle
None
RAS316B
REQUIRED PRODUCTS
Fresh blood sample
INTERVENTION TIME
15 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Dismantling
Mounting
WARNING
Pentra 60C+

Page 2/6
RAS316B
Step by step analysis cycle introduction
A-Introduction
The main cycle consists of four principal phases:
1 - Preparation Of The Sample Phase: Dilutions

2 - Count And Measurement Of The WBC Group
3 - RBC/PLT Group Count
4 - Filling Of Tank
5 - Rinsing Of The RBC/PLT Count Chamber
B-Start cycle condition

Run a startup cycle
Needle in the sampling position and green LED lit (Diag.1)
All chambers (execpt Rinse chamber) are filled with diluent (Diag.2)
Reagents syringe & LMNE syringe down (Diag.3)
Draining & Counting syringe down (Diag.3&4)
Diag.2
Diag.1
A special cycle is required when no other cycle is started during RBC/PLT count or during the filling up
of the tank:
Draining
Syringe
Counting
Syringe
Diag.3
LMNE
Syringe
Reagents
Syringe
Diag.4
Page 3/6

C-Step by step analysis cycle
1 - Preparation Of The Sample Phase: Dilutions
1
First series of dilutions:
Press sampling bar, all the chambers are drained (Draining syringe).
Blood sample of 53 l (Sampling syringe).
Transfer of the needle toward the rinsing chamber (Sampling needle motor, Carriage motor).
Distribution of 3 l of blood during the rinsing of the needle (Sampling syringe, Reagents syringe).
Transfer of the needle from rinse chamber to the first dilution chamber (Sampling needle motor,
Carriage motor).
Distribution of 10 l of blood into a flux of 1.7 ml of diluent (Sampling syringe, Reagents syringe).
Pentra 60C+
Transfer of the needle from the first dilution chamber to the WBC and BASO count chamber (Sampling
needle motor, Carriage motor).
Distribution of 10 l of blood into a flux of 2 ml of BASOLYSE ll (Sampling syringe, Reagents syringe).
Transfer of the needle from the WBC and BASO count chamber to the LMNE mixing chamber
(Sampling needle motor, Carriage motor).
Distribution of 25 l of blood into a flux of 1 ml of EOSINOFIX (Sampling syringe, Reagents syringe).
Transfer of the needle from the LMNE mixing chamber to the rinse chamber (Sampling needle
motor, Carriage motor).
Distribution of the 5 l of residual blood during the double rinsing (interior and exterior) of the needle
(Sampling syringe, Reagents syringe).
REALISATION OF THE LMNE DILUTION

The dilution in the LMNE chamber is completed by 1 ml of diluent after 12 seconds of
incubation:
Positioning of the needle opposite the distribution nipple for the EOSINOFIX.
Control of valve 8 then set the syringe group for a dose of 1 ml.
Simultaneously: Setting of the distribution syringe to distribute 25 l of blood.
Stop valve 8.
Incubation for 12 seconds.
Control of valves 1 and 3 and setting of the LMNE injection syringe to distribute 1 ml of
the stop diluent.
Stop valves 1 and 3.
Page 4/6
RAS316B
Second dilution for the RBC and the Plt:
Transfer of the needle from the rinse chamber to the first dilution chamber (Sampling needle
motor, Carriage motor).
Sample of 42.5 l of the first dilution (Sampling syringe).
Rinsing of needle exterior (in the first dilution chamber) with 0.4 ml of diluent (Reagents syringe).
Distribution of the 42.5 l of first dilution into a flux of 2 ml of diluent (Sampling needle motor,
Carriage motor).
Distribution of 0.5 ml of diluent from the interior of the needle (Sampling needle motor, Carriage
motor).
NOTE
In the case of this RBC/Plt dilution, it is the entire blood sample that is
distributed (and not a portion as in the preceding dilution). The distribution in a flux is kept in order to benefit from the homogenisation
aspect of the procedure.
In order to attain the hemoglobin measurement we add 0.4 ml of lysing
agent into the first dilution chamber immediately following the blood
sample for the RBC/Plt dilution.
2 - Count And Measurement Of The WBC Group

LMNE: Loading of the vacuum injection circuit of the optical bench (Draining syringe, control of
valves 4 and 5).
Start flux in the optical chamber (approximately 2 seconds).
Injection, acquisition of the matrix and WBC count for 12 seconds.
Simultaneously: WBC and BASO counts: Rinsing of the counting head (Diluter pushed into position, control of valves 9, 10, 21, 23 and 13).
Start the vacuum and first part of count (duration of 6 seconds, control of valve 23).
Rinsing of counting head and adjustment of the vacuum count.
Transfer of the needle from the first dilution chamber to the RBC and Plt count chamber (Sampling
needle motor, Carriage motor).
Second part of count (duration of 6 seconds).

After this first series of measurements:
- Measurement of the hemoglobin.
- Rinsing of the WBC/BASOS count chamber with a mixture of 1.06 ml of ABX Cleaner and 1.44
ml of ABX Diluent.
- Rinsing of the WBC/BASOS counting head.
Page 5/6

3 - RBC/Plt Group Count
Rinsing of the RBC/Plt counting head. Start the vacuum for the RBC/Plt counting head.
First series of counts (duration of 5 seconds, control valve 14).
Rinsing of the RBC/Plt counting head and adjustment of the vacuum.
Second series of counts (duration of 5 seconds).
NOTE
P.R.F. Process (Permanent Rinse Flow):
The WBC/BASOS and the RBC/Plt counting heads are in series during
the RBC/Plt count. The count flux from the WBC chamber circulates
Pentra 60C+
in the RBC/Plt count head and limits the possibility of the circulation
of the RBC behind the count orifice.
Autocleaning of the WBC ruby:
The P.R.F. allows as well an auto cleaning of the WBC/BASOS inasmuch
as a UNICLEAN/DIL (cleaning solution) mixture is drawn through the
WBC/BASO ruby during the entire duration of the RBC and Plt counts.
4 - Filling Of Tank
At the end of the RBC/Plt count, the RBC count vacuum is finished and used for the filling up of the
diluent tank (control valves 15 and 25).
In order to improve the filling speed, the syringe is initialised for a few seconds then drawn again to
complete the filling.
A float located in the tank controls the level of liquid.
5 - Rinsing Of The RBC/PLT Count Chamber

For reasons of maximising the mechanical resources, the rinsing of the RBC/Plt chamber is normally
programmed for the beginning of the succeeding cycle (which can be started during the RBC and Plt
count).
If no other cycle is started during RBC/Plt count or during the filling up of the tank, a special rinsing
sequence is initiated. The sample signal light changes to red and the instrument is jammed for a few
seconds.
This sequence involves draining of the RBC chamber followed by a refilling with the diluent.
Page 6/6
Optical bench Dismantling & Replacement

Procedure
CONCERNS
Instrument control:
Dismantling, Replacement and Control of Optical Bench
Hexagonal keys
Flat screw driver
Torx keys
RAS317B
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
REQUIRED TOOLS

Optical bench
Dismantling & Replacement
Control
WARNING
Pentra 60C+
Page 2/6
RAS317B

Open the left side panel and the mother board door (lock it).
WARNING
A flat cable is connected at the back of the main board, be carefull
to not disconnect it, or damage it, when you open the door.
Open the cover.
Disconnect tubes from LMNE syringe:
- Inlet 1
- Inlet 5
Disconnect tubes from valves:
- 1 Inlet 1
- 4 Inlet 2
Disconnect tube from ground fitting (Diag.1)
Diag.1
Disconnect following connections (Diag.2):

- Lamp supply.
- LMNE CIS & LMNE OD coaxes.
- Optical pre-ampli board supply.
- Optical bench grounding wire.
Diag.2
Optical bench
Page 3/6

Remove gently the Optical bench.
Untighten the silent blocks (5mm allen key) (Diag.3):
Pentra 60C+
Diag.3
Page 4/6
Locate new Optical bench.

Tighten the silent blocks (5mm allen key) (Diag.3).
NOTE
Do not tighten silent blocks too hard, check that optical bench Cannot
be lifted.
Connect all tubing and wiring as previously disconnected.
RAS317B
Open Optical bench cover (Diag.4):
Diag.4
Check that gap between emission gun and flowcell is around 3mm (Diag.5):
WARNING
Becareful, if you use a 3mm allen key to control gap, that you dont
damage flowcell or emission gun
Diag.5
WARNING
This adjustment is factory made and must not be changed
Control
A-Emission gun location control
Page 5/6

B-Optical bench lamp alignement control
Open Optical bench cover (Diag.6):
Pentra 60C+
Diag.6
To control lamp put a piece of white paper between reception gun and flowcell (Diag.7):
Diag.7
Check that projection of lamp filament is vertical (Diag.8):
Diag.9
Diag.8
WARNING
A small screw (1.2mm allen key) allows adjutment of lamp (Diag.9).
This adjustment is factory made and must not be changed.
Page 6/6
Barcode reader configuration

Procedure
CONCERNS
REQUIRED TOOLS
None
RAS318B
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min.
21/11/00
FREQUENCY
On request

None
Pentra 6 0 C+
Barcode Reader test and configuration

Barcode reader test
Barcode reader setup (DATALOGIC DLC6165-M1 P/n: XBA453A)
WARNING
Pentra 60C+

Page 2/4
RAS318B
Barcode reader test
Check that barcode reader is working properly by means of the reading test (Diag.1):
INTERLEAVED 2 OF 5 (no check digit control)
1224488
reading 12244881
without C.D.
121314151617
reading 121314151617
CODE 39 (no check digit control)

with C.D.
reading 12345ABCDE
without C.D.
12345ABCDE
reading 12345ABCDEW
CODE 128 (control without check digit transmission)
CODABAR (no egality control between start/stop character)
with C.D.
37859
reading 37859
Diag.1
123456/$
reading 123456/$
Page 3/4

Barcode reader setup (DATALOGIC DLC6165-M1 P/n: XBA453A)
An audible beep occurs after each reading. Read all the labels from top to bottom and from left to right
When the last label is read, check once more on the test labels (Diag.1).
WARNING
For code I2 of 5, to avoid a bad interpretation, it is mandatory to hold
the reader in order to read the entire code.
If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as follows:
Pentra 60C+
$+$* Restore system default configuration
$+ Enter configuration environment
CP507 WEDGE for IBM AT - ALT mode
AZ0 Disable all family codes
AB11AB*0ll6 Code39, no C.D. , 1 to 16 char
AC110416 ITF, no C.D. , 4 to 16 char
AD111AD*0316 Codabar, no start/stop, 3 to 16 char
AI11 Code128, C.D. control without transmission
EA10 no Terminator
ED3 4 good reads before accepting code
$- Exit and save configuration
Page 4/4
Power supply replacement

Procedure
CONCERNS
Instrument dismantling:
Power supply
Hexagonal keys
Flat screw driver
RAS319B
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min.
15/02/04
FREQUENCY
On request

Power supply: DBN 004 A
Pentra 60C+
REQUIRED TOOLS

WARNING
Pentra 60C+

Page 2/5
RAS319B
Disconnect power supply cable
Open the left side panel
Open the cover
Disconnect , on the mother board, the printer RS flat cable in J2 (Diag.1):
With a flat screwdriver disconnect, on the mother board, the supply connector in J37 (Diag.2):
Diag.2
Open the left side panel and the main board door (block it).
Disconnect the Optical bench lamp supply cable (Diag.3):
WARNING
A flat cable is connected at the back of the main board, be carefull to

not disconnect it, or damage it, when you open the door.
Diag.1
Diag.3
Page 3/5

Unscrew the 2 CHC M3x6 back screws of the power supply (Diag.4):
Diag.4
Pentra 60C+
Unscrew the 2 CHC M3x6 front screws of the power supply (Diag.5):
Diag.5
Remove the power supply.
WARNING
Becareful to the lamp power supply cable when you remove the power
supply.
Reassemble following previous steps backward.
Page 4/5
RAS319B
Check following voltages (Diag.6):
TEST
TP40
TP41
TP42
TP43
POINT
DESIGNATION
+5V Power supply

+12V Power supply
+24V Power supply
-12V Power supply
TARGET
+5V
+12V
+24V
-12V
Diag.6
Page 5/5
Heater assy replacement

Procedure
CONCERNS
Instrument dismantling:
Replacement of the heater assy
Hexagonal keys
Flat screw driver
Torx keys
RAS320B
REQUIRED PRODUCTS
None
INTERVENTION TIME
1h.
15/02/04
FREQUENCY
On request

Heating coil: XDA625BS
Pentra 60C+
REQUIRED TOOLS

WARNING
Pentra 60C+
Page 2/8
RAS320B
To access Heater assy you need to remove the Front panel, follow RAS 306 Front panel dismantling
of this manual.
Pull the Reagents syringe and the LMNE syringe to free space behind (no need to disconnect tubes).
Unscrew several turns the 4 silent block screws of each syring (Diag.1):
Unscrew the 2 CHC M3x6 screws shown on next diagram (Diag.2)
Diag.1
Diag.2
Disconnect following tubes on the Chambers assy:

- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
Page 3/8

The heater assy is free, let it lay on the frame and by the front aperture disconnect following tubes:
- From valve 22_2 (rep.1 on heater)
- From T4 (rep.5 on heater)
Pentra 60C+
Diag.3
The heating system is now as shown on diagram 4:
Diag.4
Page 4/8
RAS320B
Disconnect heating system wiring on the mother board plugged in J31: (Diag.5):
Remove the cable through the instrument.

Remove the heater assy and drain it over absorbant paper:
Diag.5
Diag.6
Page 5/8
Pentra 60C+
Take new Heater assy and cut tubes as follow:

mark:
Length:
Destination:
Exit 1
1.52x260
Valve 22_2
Exit 2
1.52x120
DIL1 chamber inlet 1
Exit 3
1.52x500
Valve 8_2
Exit 4
1.52x120
LMNE chamber inlet 3
Exit 5
1.52x160
side of T4 (2.3)
Side of T4
1.52x250
Valve 1_2
Center of T4
1.52x120
Valve 24_1
Exit 6
1.52x120
LMNE chamber inlet 2
Exit 7
1.52x200
Valve 22_1
Exit 8
1.52x120
RBC chamber inlet 1
Exit 9
1.52x60
Exit 11 of heating coil
Exit 10
1.52x500
Valve 11_2
Exit 11
1.52x60
Exit 9 of heating coil
Exit 12
1.52x120
BASO chamber inlet 2
Page 6/8
Note:
Already at good length
Cut 40mm.
Cut 50mm.
Cut 40mm.
Cut 50mm.
Cut 40mm.
Cut 40mm.
Cut 75mm.
Cut 40mm.
Diag.7
Put new heater on the instrument frame and connect following tubes:
FROM
1
3
5
7
10
TO
Valve 22 Inlet 2
Valve 8 Inlet 2
T4
Valve 22 Inlet 1
Valve 11 Inlet 2
FROM
2
4
6
8
9
11
12
TO
Diluent chamber: DIL 1

LMNE chamber: LMNE 3
LMNE chamber: LMNE 2
RBC chamber: RBC 1
rep.11 of the heater
rep.9 of the heater
BASO chamber: BASO 2
RAS320B
Dismantle chamber assy to get the tubes through the chambers support (Diag.8):
Lift the heater assy, make the 5 tubes go through the chambers support steel sheet (Diag.9):
Diag.9
Screw back the 2 heater assy screws (Diag.10):
Diag.8
Diag.10
Page 7/8

Put back the chamber assy and connect the following tubes on chambers:
- BASO 2
- RBC 1
- LMNE 2 & 3
- DIL 1
Pentra 60C+
Connect heating system wiring on the mother board in J33: (Diag.11):
Diag.11
Push back the Reagents syringe and the LMNE syringe and screw silent blocks (check that no tube is
disconnected).
Put back the Front panel, follow RAS 306 Front panel dismantling of this manual.
Check chambers position, follow RAS 312 Chambers Adjustment of this manual.
When startup and prime are done check that there is no leak.
Page 8/8
Pentra 60 C+
TRAINING SLIDES
Pentra 60 C+
EXPLODED VIEWS
Pentra 60 C+
SPARE PART LIST
SPARE PART LIST ...................................................................... 11-2
Pentra 60 C+
SPARE PART LIST
REF
DESIGNATION
CAE006A
CAE010A
SWITCH, MICROSWITCH XC5-81-82

SWITCH, MICROSWITCH XC5-81
CAX005A
CBK043A
CBK044A
PHOTOMETER, HGB PHOTOCELL

PCB, QWERTY KEYBOARD P80/P60C+
PCB, MOUSE P80/P60C+/P400
CBK045A
CCC006A
DAC024A
PCB, AZERTY KEYBOARD P80/P60C+

PCB, PC MONITOR P60C+
CABLE, PRINTER MIC/P60/P80
DAC025C
DAC029B
DAD075A
CABLE, L=1.5M FOR SIDE PC P60C+

CABLE, L=2.9M FOR SIDE PC
CABLE, LIQUID VALVE L=350 10F14
DAD078A
DAD112A
DAD113A
CABLE, RS232 OUTPUT

CABLE, CARRIAGE BOARD P60C+/P80
CABLE, MOTOR RELAY
DAJ007A
DAL008A
DAM009A
OPTICAL, LAMP FOR BENCH P60/P80

MOTOR, STEPPER MICROS/P60
VALVE, SOLENOID PIERC. BLOCK
DBE014A
DBN004B
DZZ018A
CABLE, BUSHING D=3, 2 BLACK

PCB, POWER SUPPLY BLOCK P60
CABLE, FERRITE FOR COAXIALS
PACK OF 20
EAC008A
EAC008A
EAC010A
FITTING, ANTI ROTATION WASHER

FITTING, ANTI ROTATION WASHER
FITTING, LUER FEMALE I=3MM
PACK OF 5
PACK OF 5
PACK OF 5
EAC010A
EAC019A
EAE005AS
FITTING, LUER FEMALE I=3MM

FITTING, LUER MALE I=3MM
TUBING, TYGON 1, 016(0, 040) L=2M
PACK OF 5
PACK OF 5
EAE006AS
EAE007AS
EAE009AS
TUBING, TYGON 1, 295(0, 051) L=2M

TUBING, TYGON 1, 52(0, 060) L=2M
TUBING, TYGON 2, 29(0, 090) L=2M
EAE011AS
EAE025AS
EAE026AS
TUBING, CRYSTAL 3X6 L=10M

TUBING, SILICON 1, 5X3, 5 L=10M
TUBING, SILICON 2X4 L=10M
EAE028AS
EAE033AS
EAE034AS
TUBING, CRYSTAL 4X6 L=2M

TUBING, TYGON 1, 143(0, 045) L=2M
TUBING, TYGON 2.54(0, 100) L=2M
EAE051AS
EBB057AS
FAA013A
TUBING, SILICONE 4, 5X8 L=2M

FILTER, RUBBER CAP DEBRIS 13MM
ORING, NEEDLE RINSE BLOC C+/80
PACK OF 10
FAA017A
FAA040A
FAA055A
ORING, TANK MIG+WASTE MIC

ORING, 5DIFF SYRINGE D=12, 1
ORING, SAMPL. NEEDLE CT/C+/P80
PACK OF 10
PACK OF 10
PACK OF 10
FAA057A
ORING, PIERC NEEDLE P120/C+/80
PACK OF 10
11-2
SPECIFIC PACKAGING
Spare part list
REF
DESIGNATION
FAA064A
FAA065A
ORING, SAMPL. SYRINGE P60/P80

ORING, REAGENT SYRINGE D=6, 3
PACK OF 10
PACK OF 10
FAA066A
FAA067A
FAK001A
ORING, DRAINING CHAMBER P60/80

ORING, 5DIFF SYRINGE D=2, 4
CHAMBER, APERTURE 50
PACK OF 10
PACK OF 10
FAK003A
FAL009A
FAL010A
CHAMBER, APERTURE 80
SILENT BLOC, FOR SUB ASSEMBLIES
SILENT BLOC, OPTIC BENCH P60/80
FAM004A
FBH016A
FBL001A
COVER, PLASTIC FOOT FOR CHASSIS

COVER, DUST COVER PENTRA60
REAGENT, REAGENT CAP 2 HOLES
FBR011A
FBR012A
FZZ024A
BELT, NEEDLE L=364 P60/P80

BELT, CARRIAGE L=544 P60
DOOR, PUSH PUSH P60
GAK302A
GAL094A
GBC015A
REAGENT, BOTTLE STOPPER D=40

CHAMBER, WAST.P120/DIL TANK P60
CLIP, MIX CH. HOLDER MIC/P120
GBC030A
GBC031A
GBG003A
SYRINGE, REAG PISTON P60/SPS/80

SYRINGE, LYSE PISTON MIC/P60/80
CHAMBER, DRAINING 1/4 TURN P60
GBG007A
GBG013A
GBG022A
CHAMBER, DRAINING CHICANE P60

CUP, OVERFLOW T ROOM
CHAMBER, T ROOM COVER P60
GBG033A
GBG034A
GBG035A
SYRINGE, REAG BLOC BODY P60/P80

SYRINGE, 5DIFF BLOCK BOTTOM P60
SYRINGE, REAGENT HOLDER PLATE
GBG037A
GBG038A
GBG040A
SYRINGE, 5DIFF BLOC BODY P60/80

SYRINGE, 5DIFF BOTTOM PLATE
SYRINGE, 5DIFF PISTON P60/P80
GBG042A
GBG044A
GBG045A
SYRINGE, CROSSPIECE P60/SPS/P80

SYRINGE, SAMPL.SYR. BODY P60/80
SYRINGE, SAMPLING BOTTOM PLATE
GBG048A
GBG082A
GBG093A
SYRINGE, SAMPLING CROSSPIECE

NEEDLE, BLOCK SHAFT
MOTOR, PULLEY P60
GBG130A
GBG138A
GBG145A
COVER, TOP COVER P60

DOOR, KEY FOR T ROOM P60
REAGENT, STRAW D=20 P60/P80
GBG147A
GBG155A
GBG157A
CUP, OVERFLOW REAGENTS SYR. P60

REAGENT, BOTTLE STOPPER D=25
CHAMBER, COUNTING HEAD P60/P80
11-3
SPECIFIC PACKAGING
PACK OF 12
PACK OF 12
PACK OF 8
PACK OF 10
PACK OF 3
PACK OF 12
PACK OF 10
PACK OF 4
PACK OF 4
Pentra 60 C+
REF
DESIGNATION
GBG158A
GBG169A
CARRIAGE, HORIZ. STOP BAR P60

NEEDLE, PIERCING P60C+/P80
GBG176AS
GBG181A
GBG188A
CARRIAGE, NEEDLE STOP P60C+/P80

CARRIAGE, SAMPLING NEEDLE STOP
SAMPLING, SAMPLE TUBE STOP C+
GBG199A
GBG202A
GBG214AS
COVER, SAMPLING HOLDER DOOR C+

SAMPLING, DOOR LOCK P60C+/ACTCP
SAMPLING, STD TUBE HOLDER P60C+
GBG215AS
GBG218A
GBG219A
SAMPLING, OPT TUBE HOLDER P60C+

CARRIAGE, VERTICAL STOP BAR C+
ORING, ANTI EXTRUSION RING
GBG245A
GBG272A
GBG273A
REAGENT, STRAW D=28 P60/P80/ACT

SYRINGE, WASTE PUMP BODY P60/80
SYRINGE, VACCUM PUMPBODY P60/80
GBG275A
GBG292A
GBG296A
ORING, APERTURE D=0, 5 P60/P80

NEEDLE, PIERC. RINSE BLOC C+/80
NEEDLE, GUIDE P60C+/P80
GBG297A
HAE026B
HAE027B
NEEDLE, RINSING BLOCK P60C+/P80

STICKER, VALVES JOUCO (1-10)
HAE028B
HAE029B
HAW002A

STICKER, FOR REAGENT DOOR P60
HAW003A
HAX0017
LAD002BS
STICKER, REAGENTS NAMES P60

STICKER, FRONT PANEL P60C+
TOOL, LATEX RBC/PLT (2 x 2ML)
MAB090A
NAA068A
RAA021B
TOOL, TORX KEY T10

STICKER, FOR TUBING (169 ITEMS)
MANUAL, TECHNICAL P60C+
RAB092D
RAB110B
RAB111B
MANUAL, USERS PENTRA60C+ GB

MANUAL, USERS P60C+ I
MANUAL, USERS PENTRA60C+ SP
RAB112B
RAB119B
RAB187A
MANUAL, USERS PENTRA60C+ D

MANUAL, USERS PENTRA60C+ P
MANUAL, USERS PENTRA60C+ DN
RAB188A
RAB189A
RAC053C
MANUAL, USERS PENTRA60C+ SW

MANUAL, USERS PENTRA60C+ G
MANUAL, USERS PENTRA60C+ FR
XAA423BS
XAA427BS
XAA429AS
PCB, PREAMP+MECA ASSY PENTRA60

PCB, LINK BOARD PENTRA60
PCB, LED BOARD FOR COVER P60/80
XAA472DS
PCB, MAIN BOARD P60 OT/C+ V2, 01

11-4
SPECIFIC PACKAGING
PACK OF 3
PACK OF 12
PACK OF 10
PACK OF 10
PACK OF 10
PACK OF 10
Spare part list
REF
DESIGNATION
XAA504A
XAA505A
PRINTER, LEXMARK E210 220V

PRINTER, LEXMARK E210 110V
XAA507A
XAA508A
XAA524A
PRINTER, EPL5900 220V P60C+/P80

PRINTER, EPL5900 110V P60C+/P80
PRINTER, EPL6100 220V
XAA525A
XBA144A
XBA193A
PRINTER, EPL6100 110V

CABLE, REAGENT GROUND FITTING
CABLE, COAX BASO-O.D LMNE-LMNE
XBA389A
XBA390A
XBA391A
PHOTOMETER, HB CPTE P60/P80

MOTOR, FOR SYRINGE BLOCKS P60
MOTOR, CARRIAGE PENTRA60
XBA392A
XBA393A
XBA394A
MOTOR, SAMPLING NEEDLE PENTRA60

FAN, MAIN FAN 24V P60/P80/P400
SENSOR, CARRIAGE MOVE PENTRA60
XBA395A
XBA396A
XBA398B
SENSOR, DRAINING DETECTION P60

SENSOR, SAMP NEEDLE MOVE P60/80
CABLE, COAX RBC/WBC P60/C+/P80
XBA399C
XBA403A
XBA404A
FITTING, GROUND FLOWCELL P60/80

TUBING, SHIELD REPARTITOR P60
CABLE, SAMPLING BLOCK P60C+
XBA411A
XBA421B
XBA453A
MOTOR, PIERCING MECHA P60C+/P80

CABLE, LED PCB/MAIN BOARD P60C+
PCB, BARCODE EXTERNAL P60C+/P80
XBA490A
XBA547A
XBA548A
CABLE, LMNE FLOWCEL CATHOD WIRE

MOTOR, FOR SAMPLING SYRINGES
MOTOR, FOR 5DIFF SYRINGE
XCA166A
XCA167A
XCA191A
CHAMBER, ISOLATOR (LONG)

CHAMBER, ISOLATOR (SMALL)
CHAMBER, ISOLATOR VENT. P80/C+
XDA481B
XDA483B
XDA555AS
VALVE, LIQ. 2WAYS/NC W/O COIL

VALVE, LIQ. 3WAYS W/O COIL
TOOL, FLOWCELL ADJ. KNOB
XDA591AS
XDA592AS
XDA593AS
SYRINGE, 5DIFF ASSY P60/P80

SYRINGE, REAGENT ASSY P60/P80
SYRINGE, SAMPLING ASSY P60/P80
XDA595AS
XDA600A
XDA601BS
MOTOR, BLOCK FOR REAG. SYR. P60

OPTICAL, BENCH PENTRA60
CHAMBER, LMNE FLOWCELL P60/P80
XDA602C
XDA605A
XDA609B
CHAMBER, 4 CHAMBERS BLOC P60/80

CHAMBER, DIL. TANK ASSY P60/P80
SAMPLING, MECHANISM ASSY P60C+
XDA610C
CHAMBER, WBC/BASO CPTE P60/P80

11-5
SPECIFIC PACKAGING
Pentra 60 C+
REF
DESIGNATION
XDA611C
XDA612CS
VALVE, LIQ 11 VALVE ASSY (1-11)

XDA613CS
XDA614CS
XDA615CS

XDA616AS
XDA617AS
XDA621A
NEEDLE, 190L SYR. P60/SPS/P80

NEEDLE, 100L SAMPL SYR. P60/80
ORING, VAC/WASTE PUMP + WASHER
XDA622A
XDA623AS
XDA624AS
ORING, REAGENT SYR. + WASHER

REAGENT, BLOCK EQUIPED P60/P80
DOOR, FOR T ROOM EQUIPPED P60
XDA625AS
XDA625BS
XDA626AS
HEATER, BLOCK COMPLETE P60/P80

HEATER, BLOCK COMPLETE P60/P80
CHAMBER, DIL.TANK COVER P60/P80
XDA629AS
XDA644AS
XDA654A
COVER, REAGENT DOOR EQUIPED P60

COVER, LEFT SIDE EQUIPED P60
SAMPLING, PIERCING BLOCK P60C+
XDA655BS
XDA655DS
XDA657A
NEEDLE, SAMPL. NEEDLE P60C+/P80

NEEDLE, TEFLON SAMPL. NEEDLE P60C+/P80
CHAMBER, 5 CHAMBERS BLOCK P60
XDA657C
XDA692A
XDA693A
CHAMBER, 5 CHAMBERS BLOC P60/80

TUBING, DILUENT ASPIRATION P60
TUBING, WASTE P60
XDA725A
XDA725B
XDA756CS
SAMPLING, MECHANISM ASSY P80/C+

SAMPLING, MECHANISM ASSY P80/C+ NEW CAME
XDA757AS
XDA758AS
XDA759AS
CARRIAGE, NEEDLE CARRIAGE P60C+

VALVE, SOLENOID PIERC. BLOC C+
COVER, FRONT COVER EQUIPED C+
XDA812A
XDA824AS
XDA826AS
KIT, T ROOM FAN + HARNESS P60

MOTOR, BLOCK SAMPL. SYR.P60/P80
MOTOR, BLOCK 5DIFF SYR. P60/P80
XDA827AS
XDA828AS
XDA832AS
SYRINGE, WASTE CPTE P60/C+/P80

SYRINGE, VACUUM CPTE P60/C+/P80
SYRINGE, 5DIFF BLOCK+MOTOR ASSY
XDA834AS
XDA930A
XEA018A
SYRINGE, SAMPL BLOCK+MOTOR ASSY

CARRIAGE, PIERCING ASSY
REAGENT, STRAW DILUENT L=360
XEA286AS
XEA381A
XEA410AS
KIT, ORING+WASHER P60/P80/P120

GREASE, AIR+LIQ. SYRINGE MICROS
KIT, TYGON TUBINGS
XEA486BS
KIT, MAINTENANCE 1 YEAR P60/P80

11-6
SPECIFIC PACKAGING
PACK OF 12
PACK OF 10
Spare part list
REF
DESIGNATION
XEA486CS
XEA487AS
KIT, MAINTENANCE 1 YEAR P60/P80

KIT, T ROOM FAN+HARNESS P60
XEA488AS
XEA602C
XEA616AS
KIT, HEATER HARNESS P60/P80

KIT, INSTALLATION P60C+
KIT, FLOWCELL INJECTOR TUBE P60
XEA629AS
XEA630CS
XEA663A
KIT, MAINT. P60 C+ 6 MONTHS

KIT, PISTONS + NEEDLE P60C+
CABLE, COAX LMNE FLOWCEL P60/80
XEA665AS
XEA701AS
XEA708AS
KIT, V1.61 PC P60C+

KIT, V1.7 PC P60 C+
KIT, SOFT SETUP PC FOR P60C+
XEA710BS
XEA726BS
XEA727AS
KIT, MAINT.6 MONTHS P60/P80

KIT, V2.21PC + V2, 11 SYST P60C+
KIT, PC MEMORY EXTENSION P60C+
XEA747AS
XEA770AS
KIT, PISTON + CROSS PIECE

KIT, V2.29PC + V2, 16 SYST P60C+
11-7
SPECIFIC PACKAGING

ABX Pentra 60 C Plus Analyzer Service Manual PDF

Caricato da

Informazioni sul documento

Titolo originale

Copyright

Formati disponibili

Condividi questo documento

Condividi o incorpora il documento

Opzioni di condivisione

Hai trovato utile questo documento?

Questo contenuto è inappropriato?

Copyright:

Formati disponibili

ABX Pentra 60 C Plus Analyzer Service Manual PDF

Caricato da

Copyright:

Formati disponibili

Pentra 60C+

Copyright 2004 by ABX DIAGNOSTICS

TECHNICAL NOTE SOFTWARE REVISION

This document applies to the latest higher software version.

1.2. Limited garantee

1.3. Safety precautions, electronic and moving parts

1.4. Biological risks

1.5. Instrument cleaning

Instrument internal cleaning

1.6. Graphics and symbols

In Vitro Diagnostics medical device

Caution, consult accompanying

Fragile, handle with care

Consult Instructions for use

2.4. Humidity and temperature conditions

2.5. Electromagnetic environment check

2.6. Environment protection

2.7. Transport and storage conditions

Prior to the shipping of an instrument by transporter, whatever

SPARE PART LIST .......................................................................................... XXI

1.1.3. Units ..................................................................................................

2. PHYSICAL SPECIFICATIONS ......................................................................

3. SUMMARY OF PERFORMANCE DATA ....................................................... 1-9

3.2. Linearity* ............................................................................................. 1-11

White Blood Cell

Red Blood Cell

1.1.2. CBC + 5DIFF Mode

White Blood Cell

Red Blood Cell

To select the set of units refers to Chapter 4. Instrument configuration

1.2. Throughput Analyses

1.3. Tube Identification

1.5. Workstation Computer

* Pentium II 350 Mhz (min.)

1.6. Measurements and computation

2.1. Power requirements

2.2. Operating temperature and humidity

2.3. Dimensions and Weight

* Height: approximately 516 mm (20.3 in.)

Computer dimensions: ....... * See computers manual

2.4. Minimum specimen volume

2.5. Dilution ratios

2.6. Hgb measurement

2.7. Counting aperture diameters

2.8. Reagent consumption (ml)

* for one background count only (maxi = 3)

*Source: 510K submission K030144

*Source: 510K submission K030144

3.2. Precision (Repeatability)

*Source: 510K submission K030144

Mean low levels

*Source: 510K submission K030144

3.4. Normal ranges*

*Source: 510K submission K030144

3.6. Leukocyte differential count

3.7. Sample stability study

3.8. Flags giving an analysis default

Results transmitted and printed out

4.1. Reagent specifications

4.2. Waste handling precautions

5.2. Blood specimens

5.3. Known interfering substances

RBC: Red Blood Cells (Erythrocytes)