PCR Blank

DATE

EXPT #
primers (10M)

rxn vol.s = ___l

per RXN amts:
master mix = ___X:
rxn vol.s = ___l
per RXN amts:
master mix = ___X:

DNA H2O 10X[

] MgCl2

dNTPs

DNA H2O 10X[ ] MgCl2

16,5
2,5
1,5

dNTPs
1

1,2

Taq

DMSO (opt)

Taq
0,125

DMSO (opt)

1,2

>>>

>>>

rxn vol.s = ___l

per RXN amts:
master mix = ___X:

DNA H2O 10X[ ] MgCl2

16,5
2,5
1,5
>>>

dNTPs
1

1,2

Taq
0,125

DMSO (opt)

1,2

rxn vol.s = ___l

per RXN amts:

DNA H2O 10X[ ] MgCl2

16,5
2,5
1,5

dNTPs
1

1,2

Taq
0,125

DMSO (opt)

1,2

master mix = ___X:

>>>

strips/singles/plates

polymerase = _________________

hot start

Y/N

PCR machine:
PCR profile name:
___min @ 94C
___ cycles of:
___s @ 94C
___s @ ___C
___s @ 72C

___ cycles of:

___s @ 94C
___s @ ___C
___s @ 72C

final ext:
___min @ 72C

4C soak Y / N

PUNCHES =
tube tube #
1
2
3
4
5
6
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8
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10
11
12
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16
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23
24

ID

tube tube #
25
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28
29
30
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39
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41
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43
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45
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47
48

ID

tube

tube #
49
50
51
52
53
54
55
56
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61
62
63
64
65
66
67
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72

ID