Documenti di Didattica
Documenti di Professioni
Documenti di Cultura
ISSN
0975-4393 (Print)
2349-2988 (Online)
www.anvpublication.org
REVIEW ARTICLE
ABSTRACT:
A herd management is crucial for better reproduction in dairy farm animals. The major herd infertility problems are
arising from manage mental factors. Various factors affecting the reproductive process are environment, endocrine,
nutrition, heredity and infectious which lead to conditions like anoestrus, infertility, early embryonic death, abortion,
mummification, still birth and endocrine logical imbalance or reproductive abnormalities which results into poor
reproductive efficiency. Heat detection in herd made successful breeding program following correct time performing
artificial insemination. A reproductive management of herd leads to higher conception rates in animals with
optimizing the calving intervals and culling rates in herd.
KEY WORDS: Bovine, Herd Management, Heat detection, Pregnancy diagnosis, Reproduction
INTRODUCTION:
Factors Affecting Reproduction:
Factors affecting reproductive process are environment,
endocrine, heredity and infectious which lead to
conditions like anoestrus, infertility, fetal death,
abortion, mummification, still birth and neonatal death5.
Any endocrinological imbalance or reproductive
abnormalities which results in to poor reproductive
efficiency may lead to the higher embryonic mortality.
Repeated embryonic mortality which become prolong
the inter-calving interval. Thats why its a major
limitation to exploit production potential6.
Record keeping:
The first step in establishing any form of effective
management is setting up and maintaining detailed
records for individual animals. Record keeping is one
of the most important parts of reproductive
management of the dairy herd. Accurate, simple and
complete records about the entire reproductive life of
the individual animal are required. It is important to
develop a system for collecting permanent records of
each adult cow in the herd. This facilitates extracting
data to calculate the above measures. Permanent
records should include the following information like
Cow identification number, which should also be on an
Received on 23.03.2016
Modified on 05.04.2016
Accepted on 15.04.2016 A&V Publications All right reserved
Research J. Science and Tech. 2016; 8(2):99-106
DOI: 10.5958/2349-2988.2016.00013.9
99
ear tag (preferably two tags, in case one falls off), Birth
date, Ancestry, such as dam and sire number and breed
type, may be grand dam and grand sires number and
breed type and cows breed type, Vaccination dates,
Important disease problems during rearing, Lactation
number, Dry off date of previous lactation, Calving
date, Each insemination date (this is generally the
important data that is not routinely recorded), the date
when confirmed pregnant by rectal palpation, the
estimated age of the fetus, Peak milk yield or estimated
full lactation yield and any diagnosed health problem.
Delayed puberty and sexual maturity:
Age at puberty and first conception will influence
lifetime productivity of cattle, reflected in the number
of calves7. Though puberty may be genetically
controlled yet nutrition, season and proper health
management are the critical factors governing puberty
in heifers8. The major reasons for delayed puberty and
sexual maturity are the Low plane of nutrition or
malnutrition, heavy parasitic infestations and poor care
and management at neonatal stage and onwards.
Nutrition:
Nutrition directly affects production and reproduction
in dairy animals Milk is from the mouth of the cow.
Severe negative energy balance at early post partum
and its associated metabolic alterations have shown
major cause of reduced fertility9. Nutrient either in
deficient amount or in higher amount has been shown
to be capable of altering reproduction10.
Table.1 Effect of Negative Energy Balance (Martinez et al.11)
Functions
Negative energy balance
1. Reduced GnRH, LH, glucose, insulin
Metabolic/
2. Mobilization of tissue reserves
Endrocrine
3. Reduction of Body Condition Score
(BCS)
4. Metabolic disorders
1. Low or absent estrogens
Ovarian changes
2. Delayed or absence LH peak
1. Poor estrous signs
Clinical changes
2. Poor oocyte quality
3. Early Embryonic Death (EED)
4. Short estrous cycle
5. Impaired endometrial function
1. Low pregnancy /calving rates
Breeding
2. Extended Calving Interval
consequences
3. Repeat Breeder
Heat-mount detectors:
A heat mount detector such as the KaMaR can be used.
This consists of a soft, translucent plastic dome
attached to a rectangle of canvas in which there is
placed a soft plastic vial of red dye, which is fixed with
adhesive just cranial to the base of the tail. When a cow
is mounted and the vial subjected to sufficient pressure,
i.e. at standing estrus, the vial is compressed, the dye
escapes and the dome becomes red. False-positives can
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Spinnbarkeit:
The ability of cervical mucus to be drawn in to thread
or to stretch to form threads when drawn apart. Cows
that had spinnbarkeit value of 11 cm or below did not
conceive.
Use of milk progesterone assays:
The return to estrus in non-pregnant cows can be
anticipated by the measurement of progesterone
concentrations in sequential milk samples.
Cow activity changes:
When the cow is in estrus during that time activity of
cow is change like Bellowing, reduced feed intake,
restlessness and nervous, frequent micturation, etc.
Use of closed circuit television:
Television cameras, recorders and monitors are now
much cheaper and more reliable than before. During the
night, provided that there is adequate lighting and good
animal identification, a continuous video recording can
be made of the loafing areas of the yard where cows are
housed. The herds man can then rapidly scan the
recording in the morning and identify cows that are in
estrus.
Use of dogs:
Dogs can be trained to detect odors associated with
estrus in cows. The sources of the odors are widespread
throughout the genital tract and also appear in milk and
urine.
Timing of AI:
The best time to breed the cow is in the middle or end
of standing heat. With good heat detection, follow these
guidelines when breeding your animals. A cow first
observed in standing heat in the morning should be bred
the afternoon of the same day. A cow first observed in
standing heat in the afternoon or evening should be
bred the following morning. First insemination is an
extremely important control point in reproductive
management. There are significant effects of
insemination site and inseminator on conception rate.
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Conception rate:
The conception rate (CR) is the percentage of
inseminations that are successful and result in
pregnancy. Herds can have high conception rates but
poor 100 day-in-calf and high 200 day-not-in-calf rates.
100 day-in-calf rate, this calculates the percentage of
the cows in the herd that become pregnant by 100 days
after calving. It also describes how many cows will
calve within 12 to 13 months of their previous calving.
200 day-not-in-calf rate, this calculates the percentage
of cows not pregnant by 200 days after calving. Many
cows not calved more than 15 to 16 months after their
previous calving.
Early Embryonic Mortality (EED):
Embryonic mortality is classified as Very Early
Embryonic Mortality (day 0-7), Early Embryonic
Mortality (day 7-24), Late Embryonic Mortality and
early Fetal mortality (days 24-285)19. Mortality is more
common during early than the late embryonic period,
i.e., from day 8th to 16th at the hatching of blastocyst
and initiation of elongation and commencement of
implantation20. About 80 per cent of this loss occurs
before days 16 to 17; nearly 10-15 per cent between
days 17 to 42 and 5 per cent after 42 days.
Genetic factors:
Genetic abnormalities account for approximately 10 per
cent of the embryonic losses and generally result in
pregnancy failure within the first two weeks.
Expression of lethal genes can cause death of the
embryo within the first 5 days of pregnancy. Another
genotypic factor contributing to embryonic death is an
abnormal chromosome number in some or all of the
embryonic cells that results in abnormal growth of the
embryo, and usually death within the first trimester of
gestation.
Endocrine factors:
Failure of release of LH results in delayed ovulation.
Since there is delay, the sperm cells have become
poorly viable as they do in the cow within 24 to 48
hours and because of the aged conditions of the sperm
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Genital infections:
Infection of the embryonic environment can be caused
by specific and non-specific uterine pathogens. Specific
uterine infections are caused by a number of viruses,
bacteria and protozoa that enter the uterus by the
haematogenous route or via the vagina. Non-specific
pathogens are mainly bacteria that enter the uterus by
ascending infection. Uterine pathogens may cause EM
by changing the uterine environment (endometritis) or
by a direct cytolytic effect on the embryo.
Uterine environment:
After fertilization, embryos cleave at different rates,
sometimes causing the maturity of the embryo to differ
from that of the uterus. The uterine environment may be
toxic to these embryos that are out of phase, resulting in
the death of the embryo. Maternal recognition occurs
around days 15 to 17 of pregnancy.
Effect of palpation:
Generally, rectal palpation does not affect the
survivability of embryos if palpated gently. Palpation
between days 34 and 41 of pregnancy using the fetal
membrane slip technique did not affect embryo or fetal
viability.
Other possible factors:
Age: Heifers are generally considered to have higher
pregnancy rates, and this increase seems to be
associated with less embryonic mortality than cows.
Older cows nearing the end of their reproductive life
will also have an increase in embryonic mortality.
Among dairy cattle, both early and late embryonic
losses increased among cows with increasing age.
Breed: Little difference in embryonic mortality across
breeds has been shown. Cattle are line-bred or inbred
have been noted to have an increased rate of embryo
mortality.
Early pregnancy diagnosis:
Identification of early pregnancy factor (3 days after
insemination):
Identification of early pregnancy factor/early
conception factor early pregnancy factor (EPF) is an
immunosuppressive glycoprotein associated with
pregnancy. Commercially available test kits are
available which use the dip-stick principle and can
detect early conception factor (ECF) in serum and milk
from as early as 3 days after artificial insemination,
although more accurate results are obtained if samples
are taken later at 7 to 8 days. USG (12-20 Days): There
are different types of machines available. The most
commonly used machines today are B-mode real-time,
meaning that they produce an acoustic image in real
time. They usually range from 3.5 to 7.5 MHz with
greater MHz you see more detail but have less depth
penetration that they produce an acoustic image in real
time.
Milk
ejaculation
test
(18-22
days
after
insemination):
This test is performed generally 3 hrs prior to the
evening milking in dairy cows (18 to 22 days after
insemination). Place the teat cannula in the left fore-teat
and leave it for milk flow from teat cistern. When the
milk flow ceases, a small dose (2.5 mg or 0.5 ml) of
Dinoprost (LutaIyse) is administered intravenously
through ear vein. If the corpus luteum of pregnancy is
present, alveolar milk starts to flow about one minute
later. Principle behind use of PGF2 in non-luteolytic
dose induces the release of oxytocin from the corpus
luteum which causes let-down of milk in the lactating
and pregnant cows.
Assay of pregnancy specific protein B (24 days of
gestation):
This protein has been identified in the maternal serum
of cows from 24 days of gestation the concentration is
measured by radio-immunoassay It is secreted by the
binucleate cells of the trophoblastic ectoderm and thus
its presence can be used to confirm pregnancy. At
present, it can only be measured by radioimmunoassay
(RIA) but, with the development of suitable enzymelinked immunosorbent assay (ELISA) methods also
detected.
Progesterone concentration in plasma and milk (21
days):
Since the CL persists as a result of the pregnancy, if a
blood sample is taken at about 21 days after the
previous estrus, progesterone levels remain elevated. If
the cow is not pregnant and is close to or at estrus then
the progesterone levels will be low although this is a
perfectly valid and reliable laboratory method, it has the
one disadvantage that it requires the collection of a
blood sample. Progesterone crossed the mammary
gland and appeared in milk and progesterone is very
soluble in milk fat there were higher concentrations per
unit volume in milk than in the blood or plasma.
Calving Interval:
Calving Interval can be divided in to voluntary waiting
period, interval from end of to voluntary waiting period,
interval to 1st AI (service period) and interval from 1st
AI to conception and gestation period .
103
Lameness:
Lameness is associated with increased number of
services per conception and consequently lower
conception rates to first service26. Cows that were lame
within 30 days post calving were also 2.63 times more
likely to develop ovarian cysts. The histamine and
endotoxins released during the decline of ruminal pH in
animals suffering rumen acidosis act indirectly to
destroy the microvasculature of the corium causing
laminitis. These substances can also potentiate their
affects at the neuroen docrine and ovarian level and
compromise the LH surge system Secondly, stress
induced hormones may alter the GnRH and/or LH surge
system. Finally, the degree of NEB may be greater in
lame cows and hence affect the somatotropic axis.
Lameness could reduce estrus intensity by reducing
progesterone concentrations before estrus without
affecting estradiol or cortisol milk profiles27.
Heat stress:
Among all environmental stressors, the temperature and
relative humidity are the major factors, which affect the
reproductive performance of dairy cows. Incidence of
Lowest Conception rate, longer calving to conception
and calving intervals are more during summer months.
Percentage of abortion and retained placenta were
highest for cows calving during summer. Fertility of
dairy animals is markedly declined during summer
season28. Heat stress can act in more than one way to
reduce fertility in lactating dairy cows. Heat Stress can
reduce dry matter intake to indirectly inhibit GnRH and
LH secretion from the hypothalamo-pituitary system.
However, it is not clear if heat stress can also directly
inuence the hypothalamo-pituitary system to reduce
GnRH and LH secretion. Heat stress can directly
compromise the uterine environment to cause embryo
loss and infertility.
Culling rate:
Cow replacement is a major cost of dairy production29.
Parity and stage of lactation affect the economic losses
of a culled cow30. Failure to conceive is a major reason
for cows are culled31. The risk of culling is increased
with parity. Hazard ratios of cows leaving the herd were
1, 1.51, 2.14, 2.68, 3.11, and 3.46 for parities 1 to 6,
respectively cows having single male calves had a 5 to
7 per cent greater hazard of culling than cows having
single female calves cows having twins had a 23 to 46
per cent greater hazard of culling.
104
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Buffalo
18 -24 months
24-30 months
35-40 months
Less than 50 days
Average 75 days
95-100 days
13-14 months
50% or greater
Less than 1.7
Greater than 70%
Less than 10 %
Less than 10 %
Less than 10 %
Less than 8 % of the herd or less than 25
% of the animals those were culled.
105
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