ACE® Ultra-Inert Base Deactivated HPLC Columns

ACE
HPLC Columns
Product Catalog & Application Guide

5
4
4 5
1
4
5
6
Time (min)
10
4
5
Time (min)
4 5
1
2
3
4
5
6
Time (min)
10
4
5
6 7 8
Time (min)
10 11 12 13
Ultra-Inert Base Deactivated Columns

for Reversed Phase HPLC
ACE Ultra-Inert Base

Deactivated HPLC Columns
ACE C18
ACE CN
ACE C8-300
ACE C18-HL
ACE Phenyl
ACE C4-300
ACE C8
ACE AQ
ACE CN-300
Table of Contents
ACE C4
ACE C18-300
ACE Phenyl-300
analytical inc.
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Introduction and Product Overview
Rapid Analysis and LC/MS Columns
Wide Pore (300 ) Columns for Peptides and Proteins
12
Capillary and Nano Columns
18
Preparative HPLC Columns
19
Integral Guard Columns
20
Introduction to Applications
21
Index by Application Type
22
Index by Analyte
23
Applications
25
Part Numbers
57
ACE
Ultra-Inert Base Deactivated HPLC Columns
Figure 1
Excellent Peak Shape for Basic Compounds
Column:
ACE Stationary Phases Virtually Eliminate the Negative

Effects of Silanols on HPLC Separations
ACE columns are manufactured using ultra-pure silica that has extremely
low silanol activity. This ultra-pure silica is efficiently bonded and
exhaustively end-capped using proprietary technology. The result is a
silica based stationary phase that has virtually eliminated the negative
effects of silanols on chromatographic separations.
Excellent Peak Shape for Basic and Acidic Compounds
The ultra-inert characteristics of the ACE columns make them the ideal
choice for separating polar basic compounds. When compared to other
modern base deactivated columns, ACE columns consistently produce
measurably better peak shape and column efficiency when separating
troublesome basic compounds.
Selectivity
4
6
Time (min)
10
ACE HPLC Columns offer unparalleled performance and guaranteed

reproducibility, making ACE the finest columns available in todays
market. ACE columns are available in seven bonded phases (C18,
C18-HL, AQ, C8, C4, CN, Phenyl) each offering a unique, and in some
cases, dramatically different selectivity. ACE may be the only HPLC
columns you need to solve your separation problems.
ACE C18 4.6 x 250 mm, 5 m
1.0 mL/min
Figure 3 Asymmetry Comparison
Temperature: 22C
Sample:

1. Desipramine
3. Imipramine
2. Doxepin
4. Amitriptyline
ACE Columns provide excellent peak shape and efficiency

for basic compounds over a broad mobile phase pH range,
and without having to add amine modifiers.
Figure 2 Peak Tailing Interaction
Lower Tailing
1.03
ACE C18
1.52
Luna C18(2)
1.61
HyPURITY Elite C18
Flow Rate:
1.78
Discovery C18
SilicaSilica - + - + + +
SiO +Na
XH+ XH
SiO Na
Particle
Particle
SilicaSilica - +- + + +
SiO +XH
Na + Na
SiO XH
Particle
Particle
Sample:
2.07
Symmetry C18
2.0 mL/min
Temperature: 24C
1.93
Inertsil ODS2
4.6 x 150 mm, 5 m
Mobile Phase: 80% Methanol

20% 0.05 M

Phosphate buffer, pH7
1.26
Inertsil ODS3
Conditions
Columns:
1.12
Xterra RP-18
Amitriptyline
3.05
Eclipse XDB C18
3.07
StableBond C18
Higher Tailing
1
X = Basic
Compound
X = Basic
Compound
Acidic silanols on the surface of silica stationary phase

supports can form ion-exchange sites that interact with
basic compounds. This ion-exchange interaction will often
contribute to peak retention and cause peak tailing. ACE
stationary phases virtually eliminate these interactions.
ACE Ultra-Inert Base Deactivated HPLC Columns
1.25
1.5
1.75
As
2.25
2.5
2.75
MAC-MOD recommends starting most method development projects with C8,

knowing that if more retention, and hence more resolution, is needed your next
choice is C18. Starting with C8 offers the benefit of shorter analysis times and/or
lower organic solvent use. The elution order for most compounds will be the same
on the aliphatic (C18, C8, C4) phases. If a different elution order is desired for
compound verification or to resolve matrix components, changing to a phenyl or
CN phase is far simpler than trying to change selectivity by mobile phase changes.
In many cases, the CN and Phenyl phases will offer a significant difference in
selectivity from the aliphatic phases.
3.25
The above data was obtained from the National Institute of Standards and Technology
(NIST), Certificate of Analysis for Standard Reference Material 870 - Column Performance
Test Mixture for Liquid Chromatography at the NIST Internet site http://ois.nist.gov/
srmcatalog/certificate/870.pdf in September 2002. The NIST test mixture, which is
designed to characterize general aspects of HPLC was revised in December 2002.
Figure 4
Alternate Selectivity Offered with
ACE C18, C8, C4, CN and Phenyl Phases
ACE C18
6 7 8
Time (min)
5
2 3
4
5
6
Time (min)
10
4 5
ACE C4
2
Capillary, Rapid Analysis, LC/MS and Preparative Columns
Specifications for ACE Phases
100
90
100
100
100
100
100
300
300
300
300
300
With todays increased pace of drug discovery, fast and efficient methods are the
rule. Short, narrow-bore columns are replacing the conventional 4.6 x 250 mm
versions. ACE HPLC columns are available in both 3 m and 5 m particle sizes.
Although 5 m particles are sufficient for most applications, greater efficiency can
be obtained by using smaller particles. This increased efficiency is an advantage in
shorter (< 150 mm) column lengths. Because of the excellent flow characteristics
of ACE silica, you will not experience the high back pressures often encountered
with other columns.
C18
C18-HL
C8
C4
CN
Phenyl
AQ
C18-300
C8-300
C4-300
CN-300
Phenyl-300
10 11 12 13
Need Even More Resolution? Choose 3 m ACE Columns
Phase
For highly polar compounds requiring high aqueous mobile phases, ACE AQ
is the column of choice. This C18 bonded phase with unique integral polar
functionality gives excellent retention, performance and reproducibility, even when
used with 100% aqueous mobile phases.
Pore
Size ()
ACE C8
Choose ACE AQ Columns for Polar Retention
All ACE bonded phases are available in 3 m, 5 m, and 10 m particle sizes. So

regardless of your application, you can scale up or scale down and be assured of
the same selectivity. If fast, high resolution preparative chromatography is needed,
ACE materials are available in up to 50.8 mm diameter columns!
Mobile Phase: 80% MeOH

20% 0.025M KH2PO4, pH 6.0
Flow Rate:
As a general rule, retention varies inversely with chain length of the bonded phase.
Retention: C18-HL > C18 > C8, AQ > Phenyl > C4 CN
Performance excellent peak shape for basic and acidic compounds

Selectivity available in six bonded phases
Guaranteed reproducibility complete column/batch validation
In spite of the recent advances in column
technology, peak tailing and poor column-tocolumn reproducibility continue to be problems
for some samples. Even a small amount of
silanol interaction, interaction that is difficult
to mask even with modern base deactivation
bonding techniques, can cause unacceptable
peak tailing. And subtle changes in silanol
activity, changes that column manufacturers
routinely miss, can cause significant changes in
retention and selectivity, especially when
separating polar basic compounds.
Choosing the Bonded Phase Thats Best for Your Application
Surface
Area (m2/g)
Carbon
Load (%)
Encapped
USP
Classification
3, 5, 10
3, 5, 10, 15
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
300
400
300
300
300
300
300
100
100
100
100
100
15.5
20.0
9.0
5.5
5.5
9.5
14.0
9.0
5.0
2.6
2.6
5.3
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
L1
L1
L7
L26
L10
L11
L1
L1
L7
L26
L10
L11
3
Time (min)
5
4
ACE CN
4
5
Time (min)
10
4 5
ACE Phenyl
1
2
3
Particle
Size (m)
Column:
4
5
6
Time (min)
ACE 4.6 x 250 mm, 5 m
Mobile Phase: 20% 0.025M KH2PO4, pH 6.0

80% MeOH
Flow Rate:
1.0 mL/min
Temperature: 22C
Sample:

1. Norephedrine 4. Imipramine
2. Nortriptyline 5. Amitriptyline
3. Toluene
Amitriptyline is commonly used to demonstrate

silanol activity on HPLC columns. The ACE
columns provide measurably better peak shape
and column efficiency compared to other
popular base deactivated columns.
Excellent Chemical Stability:

Acidic Conditions
At low pH, column deterioration is caused by

hydrolysis of the bonded phase, with a decrease
in retention observed. The nature of the silica
surface and bonding density are critical
parameters. Conventional bonded phases are
more susceptible to hydrolysis due to the lower
purity silica and a low surface coverage.
However, ACE phases combine an ultra high
purity silica with dense bonding techniques
to effectively prevent ligand cleavage under
such conditions.
The high stability of ACE C18, C8, C4, CN
and Phenyl phases is demonstrated in Figure 5.
After three months usage at pH 1.8, no
retention loss is observed with any ACE phase.
Even cyano bonded phases (regarded to be
most vulnerable to hydrolysis) show excellent
resistance, as also shown by Figure 6.
Figure 5 Acid Stability, pH 1.8

Conditions for Figures 5 & 6
k (phenanthrene)
22
20
Exceptional Reproducibility
Subtle changes in silanol activity are one of the

primary causes of column-to-column selectivity
changes. Base deactivated columns generally
have better reproducibility than other column
types due to fewer interactions between silanols
and polar compounds. ACE columns, by
virtually eliminating silanol interactions,
provide an outstanding level of column-tocolumn reproducibility for polar compounds.
1.0 mL/min
Temperature: 22C
Sample:

ACE Phenyl
ACE C4
1. Uracil
2. Dimethyl phthalate
3. Toluene
4. Biphenyl
5. Phenanthrene
Acidic Exposure Conditions:
Mobile Phase: 50% CH3CN

50% 0.1% TFA in H2O,

pH 1.8
ACE CN
0
0
15
30
45
Time (days)
60
75
90
Flow Rate:
1.0 mL/min
Temperature: 22C
Silica Manufacture
A series of stringent quality assurance tests are

performed in the manufacturing process. Each
batch of packing material and each packed
column are extensively tested to ensure excellent
chromatographic performance and dependable
column-to-column reproducibility.
Ultra pure reagents and strict control of the manufacturing process result
in a high purity silica with uniform surface characteristics.
Advanced bonding techniques are then employed, resulting in a range of
highly base deactivated phases that combine superb reproducibility with
excellent stability.
Figure 8a Extensive Testing Yields Fully Validated HPLC Columns - Batch Validation
Batch Validation
Every batch is extensively tested
for selectivity and surface activity with
a range of acidic, basic and neutral
molecules.
ACE HPLC columns have the most
stringent batch validation
specification of any reversed
phase material.
Figure 6 ACE CN Stability at pH 1.8

5
Day 1
Day 90
4
2
4
2
3
4
Time (min)
3
Time (min)
Figure 7 ACE C18 Stability at pH 11.0

Day 1 - N0.5 (tol) = 113,300 pl/m
Day 90 - N0.5 (tol) = 112,000 pl/m
Column:
4 5 6 7
Time (min)
9 10
ACE C18 4.6 x 150 mm
1.0 mL/min
Temperature: 22C
Sample:

Flow Rate:
Flow Rate:
10

50% H2O
ACE
4.6 x 150 mm

50% H2O
ACE C8
Excellent Chemical Stability:

Basic Conditions
At high pH, column degradation is caused by

dissolution of the silica support, resulting in
a decrease of column efficiency. Conventional
phases are more susceptible to dissolution due
to low surface coverage. Again, the ultra high
purity ACE silica coupled with unique dense
bonding technology effectively inhibits silica
dissolution (See Figure 7).
Column:

ACE C18
Fully Validated Columns
1. Uracil
3. Toluene
4. Biphenyl
5. Phenanthrene
4 5 6 7
Time (min)
9 10
Basic Exposure Conditions:


50% 0.025M pyrrolidine

in H2O, pH 11.0
Flow Rate:
1.0 mL/min
Temperature: 22C
Figure 8b Extensive Testing Yields Fully Validated HPLC Columns - Batch Validation
Figure 8c Extensive Testing Yields Fully Validated HPLC Columns - Column Validation
Column Validation
All columns are tested with a
multicomponent mixture to ensure
that excellent performance and
peak shape are obtained.
ACE
By eliminating silanol interference, mobile phase additives that can

contaminate mass spectrometers or reduce sensitivity can be avoided. For
example, TFA is often used to improve peak shape and resolution when
separating proteins and peptides by reversed phase HPLC. But, TFA in
the mobile phase will suppress electrospray ionization of analytes and
reduce sensitivity. The extremely inert character of ACE phases permits
the use of mobile phases without damaging amounts of additives.
Rapid Analysis and LC/MS Columns
20 mm, 30 mm and 50 mm column lengths

1.0, 2.1, 3.0 and 4.6 mm column diameters
Configured for High Sample Throughput
Specially Manufactured for High Flow Applications
Ultra-Inert Stationary Phases for Improved Efficiency and Sensitivity
Highly Stable Columns for Robust, Reliable LC/MS Methods
Figure 10 Independent Column Comparison of HPLC Columns
Configured for High Sample Throughput
To reduce analysis time and increase sample throughput, ACE rapid

analysis columns are short in length and packed with high efficiency
stationary phases.The shorter column length provides the rapid analysis
and the high efficiency 3 micron particles provide the resolution.
Leading 5 m C18 Column Brands

Basic Molecule Testing
Peak Asymmetry Investigation
This bar graph, generated from data produced by the National Institute of
Standards & Technology, compares columns according to peak asymmetry. The
lower the peak asymmetry, the lower the interference from silanols. The ACE C18
showed the least silanol interference of any of the C18 columns tested.
Figure 9 Fast Separations for High Sample Throughput

Ultra Inert Silica - Improved Efficiency and Peak Shape
0.0
The Right Reversed Phase HPLC

Column to Use for High Throughput
LC/MS Applications
The pressure to get new drugs to market faster

requires analytical chemistry tools that can
keep up with the frenetic pace of potential lead
compound generation during the drug discovery process. By increasing sample throughput,
not only does the cost per analysis decrease but,
more importantly, critical information can be
quickly provided to researchers to screen the
large number of compounds generated from
synthesis or natural product sources.
Combining mass spectrometry with HPLC has
given researchers a powerful analytical tool
because of the ability of HPLC to separate
complex mixtures and the sensitivity and
selectivity of mass spectrometry. The fact that
LC/MS is also well suited for high sample
throughput makes it the analytical tool of
choice for drug discovery.
8
0.5
Column:
1.0
1.5
Time (min)
2.0
2.5
3.0
ACE C18, 30 x 3.0 mm, 3 m
Mobile Phase: Fast Gradient, 5%B to 100%B, 3 minutes

A: 20mM Phosphate Buffer, pH 2.7

B: 65% Methanol, 35% 20mM Phosphate Buffer, pH 2.7
Flow Rate:
ACE Stationary Phases

Virtually Eliminate the
Negative Effects of Silanols
on HPLC Separations
ACE C18
1.03
ACE C18
1.03
1.12
XTerra RP18
1.12
XTerra RP18
Inertsil ODS3
1.26
Inertsil ODS3
1.26
Luna C18(2)
1.52
Luna C18(2)
1.52
1.61
HyPURITY Elite C18
1.61
HyPURITY Elite C18
1.78
Discovery C18
1.78
Discovery C18
Spherisorb ODS1
1.88
Spherisorb ODS1
1.88
1.93
Inertsil ODS2
1.93
Inertsil ODS2
2.07
Symmetry C18
2.07
Symmetry C18
2.21
Hypersil BDS C18
2.21
Hypersil BDS C18
3.05
Zorbax Eclipse XDB C18
3.05
Zorbax Eclipse XDB C18
3.07
Zorbax SB C18
3.07
Zorbax SB C18
7.85
Hypersil ODS
7.85
Hypersil ODS
8.92
Spherisorb ODS2
8.92
Spherisorb ODS2
11.0
Zorbax ODS
11.0
Zorbax ODS
1
2
3
4
5
6
7
8
9
10 11 12
1
2
3
4
5
6
7
8
9
10 11 12
As (Amitriptyline)
As (Amitriptyline)
ACE ultra inert base deactivated HPLC

columns virtually eliminate the negative effects
of silanols in HPLC separations. This
unequalled performance is now available in 20,
30 and 50 mm length columns, with diameters
from 1.0 to 4.6 mm ID. The ACE columns
are suitable for high throughput and LC/MS
applications, and are the ideal choice for high
volume screening assays used for drug analysis
and combinatorial libraries where robust,
reproducible columns are essential.
Apart from being the most inert HPLC
columns on the market, they are also the most
efficient, and are manufactured and validated
to the same exacting standards as all ACE
columns. Increased efficiency is an important
benefit given the short columns typically used
in LC/MS and rapid analysis applications.
2.0 ml/min
Reproduced with permission of AstraZeneca, Charnwood, UK
Lower Tailing
Lower Tailing
Higher Tailing
Higher Tailing
An ACE rapid analysis column is used with a fast gradient and at high flow rate to achieve
a separation of 10 components in a mixture in less than 3 minutes.
Ultra-Inert Stationary Phases for Improved Efficiency

and Sensitivity
Unwanted interaction between solutes and acidic silanols on the surface

of silica-based stationary phases is the major cause of poor column
efficiency and poor sensitivity when analyzing polar compounds by
HPLC.Stationary phases used in ACE HPLC columns are made
using ultra-inert silica as the stationary phase support. This silica is then
densely covered by the bonded phase so completely that silanol
interference is essentially eliminated. The result is a stationary phase that
provides superior peak shape, making it particularly well suited for high
throughput, high sensitivity LC/MS applications.
Column:
150 x 4.6 mm, 5m
Mobile Phase: 80:20 MeOH/5mM potassium phosphate buffer (pH 7.0)

Flow:
2.0 ml/min
Temperature: 24C
Data obtained from the National Institute of Standards and Technology (NIST), USA
The above data was obtained from the National Institute of Standards and Technology
(NIST), Certificate of Analysis for Standard Reference Material 870 - Column Performance
Test Mixture for Liquid Chromatography at the NIST Internet site http:/ois.nist.gov/srm
catalog/certificates/870.pdf in September 2002. The NIST test mixture, which is designed
to characterize general aspects of HPLC was revised in December 2002.
150 x 2.1 mm
0.2ml/min
1
2
50 x 2.1 mm
5
6
Time (min)
10
0.2ml/min
1
3
30 x 2.1 mm
1
5
6
Time (min)
10
0.2ml/min
Column ID
Relative Sensitivity
4.6 mm
1.0
3.0 mm
2.3
2.1 mm
4.8
1.0 mm
21
5
6
Time (min)
10
Figure 13 Effect of Tailing on Sensitivity
0.2ml/min
Leading C8 Column
ACE C8 Column
Figure 15 ACE HPLC Columns are Compatible with a Wide Range of

MS Friendly Buffers
1 2
pH 2
0.1% TFA
0.0
20 x 2.1 mm
5
6
Time (min)
10
2.0
Time (min)
2.5
3.0
3.5
4.0
0.0
0.5
1.0
1.5
2.0
Time (min)
2.5
3.0
3.5
4.0
3.0
3.5
4.0
3.5
4.0
pH 7
10mM NH4HCO3
0.0
0.5
1.0
1.5
0.8ml/min
3
1
pH 10
10mM NH4HCO3
Column:
1.4
0.1
5
6
Time (min)
0.2
0.3
Time (min)
0.4
1.6
1.8
2.0
2.2
1.4
1.6
1.8
2.0
2.2
0.0
0.5
1.0
1.5
60
2.5
3.0
10
ACE 3 C18
2. Toluene
1000
2000
3000
4000
0.5
1.0
1.5
3. Biphenyl
4. Phenanthrene
2.0
This figure compares the LC/MS signal intensity on two different brands of HPLC columns.
Because ACE stationary phases are more inert, with less silanol interference, the
chromatographic peak produced by the ACE C8 column has less tailing and, therefore,
provides greater sensitivity. The highly inert characteristics of the ACE HPLC columns give
them inherently higher sensitivity than other columns.
2.0
Time (min)
2.5
3.0
3.5
4.0
5000
Column: 50 x 3.0 mm
2.0
0.0
40
No. of Gradient Cycles
ACE HPLC columns are available in 20mm and 30mm lengths

enabling rapid, reproducible high throughput screening at elevated
flow rates.
10
2.0
Time (min)
pH 10.5
10mM NH4OH
0.5
Mobile Phase: 80:20 MeOH/H2O

Sample:

2.5
Leading Competitor 3m C8
0.0
2.0
Time (min)
ACE 3 C8
80
4
3
1
2
1.5
100
% Efficiency
1.0
1 2
Figure 14 Columns Specially Manufactured for

Long Life Under High Throughput Conditions
0
0.5
pH 3
10mM NH4COOH
ACE HPLC columns, by virtue of their high

bonding density and ultra-inert characteristics,
can be used with mobile phase conditions that
are optimal for LC/MS applications. Stability
at both low and high pH and compatibility
with volatile buffers makes these the right
columns for demanding situations such as
standard methods and open access LC/MS.
The proprietary packing technology used to
specially manufacture these columns for high
throughput applications produces extremely
durable HPLC columns that are the key to
robust LC/MS methods.
To maximize sample throughput, very high

mobile phase linear velocity (flow rate) is often
used, sometimes 3 to 4 times higher than would
be used for typical HPLC applications. This
puts extra strain on the column packing bed
and causes early failure due to voids or channels
that develop. ACE stationary phases are
mechanically very strong and able to withstand
aggressive packing techniques. This has
permitted the development of proprietary
packing technology that is designed specifically
to produce columns robust enough for high
throughput applications.
Highly Stable Columns for Robust,
Reliable Methods
As the column internal diameter decreases, the volume

of the chromatographic peaks decreases, concentrating
the sample and increasing sensitivity. If the same amount
of sample is injected, a column with an ID of 2.1 mm will
give about 5 fold the sensitivity of a column with an ID
of 4.6 mm.
20 x 2.1 mm
1
Figure 12
The Effect of Column ID on Sensitivity
3 4
ACE HPLC columns, by virtue of their inert characteristics, give

inherently higher sensitivity because of the sharp, narrow peaks produced
by their stationary phases.When there is a limited amount of sample
for analysis, which is often the case in drug discovery, sensitivity can be
increased by choosing columns with smaller internal diameters. As the
column internal diameter decreases, the volume of the chromatographic
peaks also decreases, concentrating the sample and increasing sensitivity.
Columns with 2.1 mm and 1.0 mm internal diameters are particularly
attractive for situations where the amount of sample is limited but
sensitivity is important.
Specially Manufactured for High Flow

Applications
Increased retention of basic molecules
Increased Sensitivity
Figure 11
Using ACE HPLC Columns for Fast Separations
Gradient: Fast gradient cycling

5% to 95% Acetonitrile in 2.25 minutes
Flow Rate: 1.25 ml/min
In this lifetime test under high flow and fast gradient
conditions, the specially manufactured ACE HPLC
column showed no loss in performance after over 5,000
gradient cycles. A typical fast analysis column failed to
provide acceptable performance after less than a 1,000
gradient cycles.
Column:
ACE C18, 50 x 2.1 mm, 3 m
Mobile Phase: 80% MeOH, 20% buffer

Flow Rate:
0.20 ml/min
Temperature:
22C
Sample:

1) Nortriptyline
2) Amitriptyline
Wavelength:
215 nm
ACE HPLC columns can be used over a broad pH range, allowing for simplified method
development on a single column. And, ACE columns are compatible with volatile mobile
phase buffers recommended for LC/MS applications.
11
ACE
300 Columns for Peptides and Proteins
300 ultra high purity silica

Ultimate protein and peptide application column
C18, C8, C4, CN and Phenyl chemistries
Excellent peak shape and reproducibility have
established ACE HPLC columns as the finest
available. This quality is now available for protein
chemists desiring the utmost in performance
and reproducibility for the separation of
peptides, proteins and other high molecular
weight biomolecules.
ACE 300 columns are available in an
extensive range of dimensions and particle sizes
for use in micro-scale separations, LC/MS
analyses and high speed preparative analyses up
to process scale.
Figure 16 ACE 300 Columns for Peptide and Protein Analyses

Proteins
2
Compounds
1. Ribonuclease A
2. Cytochrome C
3. Holo-transferrin
4. Apomyoglobin
10
12
14
16
18
20
22
24
26
28
Chromatographers prefer inert stationary

phases for the reversed-phase HPLC of ionic
compounds because they minimize the
negative effect of silanols on the separation.
Column:
ACE 5 C18-300, 250 x 4.6mm
Flow Rate:
1.0ml/min
Temperature: Ambient
Mobile Phase: A. 0.1% TFA in H2O

B. 0.1% TFA in MeCN 5% to 70% B in 30 mins
Detection:
This results in improved peak shape and

reproducibility when separating compounds
that contain polar functional groups, especially
amines.
exhibiting low silanol activity are most likely to give consistent results
column-to-column and batch-to-batch. In addition, polar and basic
compounds will have better peak shapes and hence greater sensitivity on
columns with low silanol activity. Since most biomolecules are polar and
many are basic, columns with low silanol activity are desirable. In Test III,
N values for tricyclic antidepressants measure metal content in addition
to active silanol activity. Amitriptyline, chromatographed at neutral pH,
is a standard test for measuring silica quality.
Conclusion
The overall ranking of the 300 columns shown in Figure 17 reflects

their performance based on how well they are packed and also the
silanol and metal activity of the stationary phase. Chromatographers
with experience in HPLC of basic pharmaceuticals know that columns
giving good results on these tests will perform best for their samples. The
benefits obtained from ultra-inert stationary phases are also important
in wide-pore columns designed for the analysis of biomolecules.
Figure 17 300 C18 Columns Ranked by Average Efficiency

20,000
15,000
10,000
5,000
0
ACE
C18-300
Jupiter
C18
Zorbax Symmetry Nucleosil Vydac

Vydac
300SB-C18 300 C18 300 C18 Everest C18 218MS
Column:
ACE 5 C18-300, 250 x 4.6mm
Flow Rate:
1.0ml/min
Vydac
218TP

Detection:
UV, 280nm
10
12
14
16
18
Time (min)
Column:
ACE 5 C18-300, 250 x 4.6mm
Flow Rate:
1.0ml/min

Detection:
In order to demonstrate the benefits of ultrainert phases in biomolecule analysis, several

commercially available 300 pore size reversedphase columns were tested using three different
samples: neutral molecules to measure efficiency,
pyridine/phenol to measure silanol activity and
antidepressants to measure both silanol activity
and metal content. These are the same test
procedures typically used to evaluate standard
pore size columns (eg 100) used for the
analysis of small molecules in the chemical
and pharmaceutical industries. Columns were
ranked by efficiency, N, measured at 10%
peak height. In addition to measuring overall
efficiency, this value also takes into consideration peak tailing usually caused by silanol
interactions. The table below summarizes the
performance of various columns as determined
by each test along with an overall ranking based
on a combination of all three tests.
Column efficiency, as measured in Test I, is a

reflection of how well a column is packed, as
well as particle size and particle size distribution. Although many columns performed
similarly in this test, those with lower plate
counts reflect poorer physical characteristics
of the silica particle. In Test II, efficiencies for
pyridine are a good measure of active silanols
on the silica surface. Active silanols account for
most peak tailing and adsorptive losses of
proteins. Since silanol activity is very hard to
control in silica manufacture, columns
Compounds
1. Gly-Tyr
2. Oxytocin
3. Angiotensin II
4. Neurotensin
Neutral molecule test for packing integrity

2 basic molecule tests for silica inertness
Peak efficiency and asymmetry comparison
Results
UV, 280nm
Peptides
A new generation of ultra-inert stationary

phases, with extremely low silanol activity, has
made it possible to achieve even better peak
shape and reproducibility when separating these
types of compounds.
12
30
Time (min)
ACE 300 Columns for Peptide and

Protein Analyses
Scientists working with small molecules have

been rapidly adopting this new technology and
wide-pore (300) ultra-inert phases makes the
benefits of this technology available to those
wanting to separate peptides and proteins by
reversed-phase HPLC.
3 m, 5 m and 10 m particle sizes

Unmatched reproducibility
Exceptional chemical stability
Comparison of Leading 300 5 m

C18 Columns
UV, 220nm
20
Efficiency Measurements (N) For Leading 300 (5 m, C18, 250 x 4.6mm) HPLC Columns
ACE C18-300
Jupiter C18
Zorbax 300SB-C18
Symmetry 300 C18
Nucleosil 300 C18
Vydac Everest C18
Vydac 218MS
Vydac 218TP
Test I
Test II
Test III
Average
23,400
19,700
18,900
17,500
20,300
20,000
14,600
14,200
14,400
12,400
14,400
9,000
6,700
5,900
1,300
1,700
14,000
12,400
6,600
6,700
400
800
1,400
800
17,300
14,800
13,300
11,000
9,100
8,900
5,800
5,600
Test I: neutral molecule - toluene

80:20 MeOH/H2O, 1.0ml/min
Test II: basic molecule 1 pyridine

60:40 MeOH/H2O, 1.0ml/min
Test III: basic molecule 2 - amitriptyline

80:20 MeOH/25mM KH2PO4 (pH 6.0), 1.0ml/min
13
The Benefits of Ultra-Inert Stationary Phases for the Reversed-Phase

HPLC of Biomolecules
Increased Sensitivity
Optimizing Selectivity
TFA or trifluoroacetic acid is used as a mobile phase additive for reversed-phase separations of
peptides and proteins. This additive is typically used to improve both the peak shape and
resolution of complex mixtures of peptides and proteins. As shown in Figure 18 , the use of 0.1%
TFA in the mobile phase enables a column packed with an active stationary phase to give peak
widths comparable to those obtained from a new generation column made from ultra-inert
stationary phase. However, as the TFA concentration is lowered to 0.01% and finally 0.005%, peak
widths on the ultra-inert phase stay the same, but degrade on the active stationary phase. The
ability to analyze peptides and proteins using very low levels of TFA is beneficial for high
sensitivity detection by mass spectrometry. TFA complexes with polypeptides and can enhance
selectivity. However, this same complexation lowers sensitivity in the mass spectrometer.
The ability of TFA and other mobile phase additives to complex with
peptides and proteins can be used to adjust selectivity and improve
resolution. As shown in Figure 19, lowering TFA concentration from
0.1% to 0.01% enabled the resolution of angiotensin II and III. In
the case of the ultra-inert ACE column, peak shape and sensitivity
remained constant with this change, as resolution improved
dramatically. In the case of the Vydac column, packed with a more
active stationary phase, peak shape was severely degraded.
Figure 18 Sensitivity and Peak Shape as a Function of TFA Concentration
Figure 19 Selectivity as a Function of TFA Concentration
ACE 5 C18-300
Ultra-inert silica
50
1
3
40
20
20
150
15
10
10
50
10
12
14
Minutes
16
18
20
22
24
10
12
14
16
18
20
22
10
10
0
4
10
12
14
Minutes
16
18
20
22
20
15
24
mV
mV
40
30
30
mV
25
4
10
12
14
16
18
20
22
24
15
10
5
10
12
14
2
3
16
18
20
22
24
10
250 x 4.6mm, 5m C18 300
Mobile Phase: A: TFA in H2O

B: TFA in MeCN
(% TFA as specified above)
10% to 55% B in 37.5 mins.
Flow Rate:
15
10
11
12
13
14
15
45
2
15
1,2
40
35
30
40
25
20
15
10
5
0
10
11
12
13
14
15
250 x 4.6mm, 5m C18 300
20
25
10
12
14
16
Minutes
Flow Rate:
1.0ml/min
Detection:
UV 215nm
Compounds: 1. Angiotensin II

2. Angiotensin III

3. Angiotensin I
Resolution has increased by lowering the TFA concentration. Columns made from lower quality silica show decreased performance.
30
35
Minutes
Minutes
Column:
14
Minutes
Mobile Phase: A: TFA in H2O

B: 80% TFA in MeCN

20% TFA in H2O

(% TFA as specified above)

25% to 40% B in 15 mins.
0
8
13
50
Column:
20
10
12
Minutes
25
15
11
40
35
10
10
45
35
Minutes
20
20
50
1
30
3
50
45
50
25
15
60
30
20
70
35
25
80
40
30
1,2
Minutes
0.01% TFA
45
35
220
200
180
160
140
120
100
80
60
40
20
0
Minutes
40
24
50
45
100
15
50
mV
25
mV
mV
mV
25
mV
200
3
30
1,2
250
35
30
0.005% TFA
300
40
35
0.01% TFA
0.1% TFA
45
mV
45
Vydac 218TP
Lower purity active silica
mV
50
0.1% TFA
ACE 5 C18-300
Ultra-inert silica
Vydac 218TP
Detection:
UV 200nm
Compounds:

1. Gly-Tyr
2. Oxytocin
3. Angiotensin II
4. Neurotensin
1.5ml/min
Columns based on lower quality silica (chromatograms on right) show a dramatic loss in performance as TFA concentration is lowered. Columns from
ultra-inert silica such as ACE maintain performance when TFA concentration is decreased.
14
15
Comparison of Leading Wide Pore Columns

Increased pH Range
Most biomolecules are charged. Peptides and proteins have numerous charges. From experience
with small molecules, it is known that mobile phase pH can be a powerful tool for changing
retention and thus optimizing the resolution of charged compounds. The same is true for peptides.
Again using angiotensin II and III as an example, Figure 20 shows no resolution of these two
peptides at pH 2 on either the ACE ultra-inert column or a column packed with a more active
stationary phase. By increasing the pH to 7, both columns now give good resolution. However,
whereas the ACE ultra-inert column maintained good peak shape, the more active column showed
poorer peak shape and a loss in performance. This phenomenon is observed in most reversed-phase
applications with polar compounds. At high pH, silanol interactions are more prevalent and hence
peak tailing becomes more apparent on active stationary phases.
ACE 5 C18-300
Ultra-inert silica
1,2
150
mV
mV
200
3
100
50
0
0
10
11
12
13
14
220
200
180
160
140
120
100
80
60
40
20
0
120
mV
mV
1 2
80
60
40
20
0
0
10
11
12
13
14
15
ACE 5 C18-300
N
(pyridine)
250 x 4.6mm, 5m C18 300
Mobile Phase: pH 2 A: 0.1% TFA in H2O

B: 0.1% TFA in MeCN

pH 7 A: 10mM NH4OAc in H2O

B: MeCN

25% to 40% B in 15 mins.
ACE 5 C18-300
3
= 53,600pl/m
(amitriptyline)
= 56,000pl/m
5
2
1
3
1
1
120
110
100
90
80
70
60
50
40
30
20
10
0
10
11
12
13
14
15
1
Time (min)
1
2
3
1
0
1
10
11
12
13
14
12
14
N(pyridine) = 7,200pl/m
15
10
Time (min)
Time (min)
15
10
N(amitriptyline) = 1,600pl/m
6
Time (min)
Vydac 218TP
Vydac 218TP
Minutes
Column:
15
Figure 22 Inertness Test 2

4
Minutes
140
100
ACE 300 columns have been repeatedly shown to be the most inert columns available.
1,2
Minutes
pH 7
Since most biomolecules are polar, and many are basic, these inertness tests can be used to
accurately predict the best column for the analysis of biomolecules, where an ultra-inert column
with low silanol activity is highly desirable.
Vydac 218TP
300
250
Significant differences in efficiency, peak shape and selectivity are seen with these 300 C18
bonded phases when analyzing basic molecules. These variations are caused by undesirable
secondary silanol interactions, which can also result in poor column reproducibility.
Figure 21 Inertness Test 1
Figure 20 Effect of Mobile Phase pH on Resolution
pH 2
Leading 300 5m, C18 column brands - 250 x 4.6mm i.d.

2 Basic silica inertness tests
Peak efficiency and asymmetry comparison
20
Minutes
Flow Rate:
1.0ml/min
Detection:
UV 215nm
Adjusting the pH of the mobile phase is a powerful tool for increasing selectivity. Only columns based on ultra-pure silica will maintain performance at
higher pH values.
Vydac Everest C18
Compounds: 1. Angiotensin II

2. Angiotensin III

3. Angiotensin I
(pyridine)
= 31,200pl/m
Vydac Everest C18
N(amitriptyline) = 3,600pl/m
5
2 3
1
0.5
1.5
3
2.5
Time (min)
3.5
4.5
12
10
Time (min)
14
16
18
20
Mobile Phase: 65:35 MeOH/25mM KH2PO4 (pH 6.0)
Flow Rate:
1.0 ml/min
Flow Rate:
1.0 ml/min
Sample:
1) Uracil 2) Pyridine 3) Phenol
Sample:

1) Norephedrine 2) Nortriptyline
3) Toluene 4) Imipramine 5) Amitriptyline
- Independently tested at The School of Pharmacy, University of Sunderland, UK
16
17
ACE
ACE
Capillary and Nano Columns
Ultra-Inert High Efficiency Columns
In addition to the extensive range of analytical (1.0-4.6mm ID) through

to preparative (21.2-30mm ID) columns, ACE columns are available
in capillary (500 m and 300 m) and nano (100 m and 75 m)
dimensions. ACE capillary and nano columns are available with all ACE
bonded phase chemistries in both 100 and 300 pore sizes. The same
features that make ACE ultra-inert base deactivated columns the choice
of method development chemists also make them the ideal choice for
capillary and nano HPLC applications.
Capillary and nano HPLC is gaining acceptance for applications where

limited sample amounts lead to problems in detection sensitivity. This is
relevant in the areas of pharmacokinetics, trace analysis and in particular
the expanding fields of bioanalytical and proteomic analysis. ACE
capillary and nano columns are ideal for use with detectors requiring very
low flow rates, such as electrospray LC/MS.
ACE capillary and nano HPLC columns offer high sensitivity due to
their low dispersion characteristics. The table below shows the
theoretical sensitivity increase of each ID column compared with a
4.6mm ID analytical column and 1mm ID microbore column. This
increase in sensitivity can be important for accurate quantitation of
sample limited applications.
For maximum performance, columns should be used with fully optimized

HPLC systems (eg. minimize system dead volume using short lengths of
small ID connection tubing).
Sensitivity Increase
Typical Flow
Rate (l/min)
Theoretical Sensitivity
Increase1
1000
40
10
3
0.5
0.3
1
21
85
235
2100
3760
For same sample mass
18
Now Achieve Reproducible High Performance

Preparative Separations
Figure 23 Reproducible Scale-up With ACE C18 Columns
Chromatographers with experience in preparative HPLC know what is

important, resolution and loadability. The two go hand in hand; the
greater the resolution, the higher the sample load, the faster you obtain
pure compound. The ability to optimize resolution at the preparative scale
means starting with high performance separations at the analytical scale.
The same features that make ACE Ultra-Inert Base Deactivated
analytical columns the choice of method development chemists also make
them the ideal choice for scale-up and process methods.
ACE 3 C18 LC/MS, 150 x 1.0 mm, 0.05 mL/min
ACE 5 C18 Analytical, 150 x 4.6 mm, 1.0 mL/min
ACE Preparative Column features:
Improved Mass Limit of Detection
4.6
1.0
0.5
0.3
0.1
0.075
Preparative HPLC Columns
Loadability - high surface area and carbon Rugged - reliable, long-term performance
load for maximum sample capacity
Guaranteed reproducibility - complete
Selectivity - available in 7 bonded phases
column/batch validation just like the ACE
to optimize resolution and maximize sample analytical columns
capacity. C18, C18-HL, C8, C4, CN, Phenyl, AQ
Capillary (500 m and 300 m) and nano (100 m and 75 m) dimensions

Wide range of bonded phases available
100 and 300 pore sizes
High efficiency, long lifetime and guaranteed reproducibility
LC/MS and LC/MS/MS applications
Column ID
(mm)
Ultra high purity base deactivated silica

5, 10 and 15 m particle sizes available
Columns are fully validated
Exceptional reproducibility
Excellent efficiencies
Reliable, long-term performance
90, 100 and 300 pore sizes
ACE 10 C18 Preparative, 150 x 21.2 mm, 21.2 mL/min
Choose the Bonded Phase Best for Your Sample
ACE preparative columns are available in 7 bonded phase selectivities

including AQ and C18-HL (Hi-Load), making it possible to optimize
your preparative resolution and in doing so, increase loadability. See
Specifications Table on page 3.
Mobile phase: 35:65 Acetonitrile/1% TFA in H2O
Temperature: 22C
Wavelength: 254nm
Sample:

1) Uracil
4) Benzoic acid
2) 4-Hydroxybenzoic acid 5) 2-Hydroxybenzoic acid
3) Acetylsalicylic acid
Get High Purity Product Fast
ACE Preparative columns are available in a variety of column dimensions

and particle sizes. For maximum loadability, choose 30 mm ID columns.
Use a 50 mm length column with a 5 m particle size to maximize
the speed of your separation. To maximize resolution, choose a
250 mm column with 5 m particle size. See the table below for
loading specifications.
Column Sample Capacity (Loadability)
Column
Size
ID
(mm)
Relative Flow
Rate (ml/min)
Weight of
Phase (g)
Analytical
Semi-Prep
Prep
Prep
4.6
10.0
21.2
30.0
1.0
4.7
21
42
3.0
14
64
130
Maximum Sample Per Injection

a < 1.1
a > 1.5
4 mg
20 mg
90 mg
180 mg
60 mg
280 mg
1300 mg
2600 mg
19
ACE
ACE
Integral Guard Columns
Guard column is incorporated into the analytical columns inlet end-fitting

Ultra low dead volume design provides protection without degrading performance
Easy to change cartridge design
ACE analytical columns are manufactured with state-of-theart column
fittings that incorporate the guard column as an integral part of the
analytical column. To install a guard column on an ACE analytical
column, simply replace the standard column inlet end-fitting with the
end-fitting designed as a guard column holder. Then, insert a guard
cartridge packed with the desired stationary phase into the guard
column holder (See photograph). Dont worry about disturbing the
packing bed when installing the guard column holder. It is well protected
by a PEEK cap, even with the column end-fitting removed.
The integral guard column system is available for columns with internal
diameters of 2.1, 3.0, and 4.6 mm. For preparative columns
(> 10 mm), the more typical stand-alone style of guard columns are
available packed with the appropriate stationary phase. See page 60 for
part numbers on preparative guard columns.
As a general rule, retention increases with chain length of

the bonded phase, that is:

Retention (R):
If for some reason you prefer to use a stand-alone style of guard column
to protect your analytical column, use part number H0001. All 1.0 mm
ID columns use a H0001 stand-alone style guard cartridge holder.
Connecting tubing and fittings, or a column coupler, will be required to
use this guard column.
Using a guard column to protect your analytical column can substantially

increase column lifetime and improve the quality of your chromatography.
But, to be effective the guard column must be replaced often enough
to prevent contaminants from saturating the guard column and bleeding
Part Number
For analytical columns 1.0 and 2.1 mm ID

For analytical columns 3.0 and 4.6 mm ID
20
Application Guide
Choosing the Bonded Phase Thats Best for Your Application
through to the analytical column. The best way

to determine the optimum time to replace a
guard column for a specific set of sample and
mobile phase conditions is through experience.
However, it is helpful to have some quantitative
measure to help make the replacement decision.
By monitoring plate number (N), pressure (P),
and resolution (Rs), the performance of the
guard and analytical column can be closely
watched to determine when a guard column
should be replaced. We suggest replacing the
guard column when any one of these parameters changes by more than 10%.
Integral Guard Holder
H0004
H0005
more retentive

less retentive
C18-HL
C18
C8, AQ
Phenyl
C4, CN
We recommend starting most method development projects

with C18 or C8, knowing that if more retention and hence
more resolution is needed, your next choice is C18-HL
(Hi-Load). Starting with C8 offers the benefit of shorter
analysis times and/or lower organic solvent use. The elution
order for most compounds will be the same on the aliphatic
(C18, C8, C4) phases. If a different elution order is required
for compound verification or to resolve matrix components,
changing to a phenyl or CN phase may be far simpler than
trying to change selectivity by mobile phase or temperature
changes. In many cases, the ACE CN and ACE Phenyl
phases will offer a significant difference in selectivity from
the aliphatic phases.
ACE AQ is particularly recommended for applications
requiring high aqueous content mobile phases. Improved
resistance to retention loss caused by pore dewetting is
seen compared to standard C18 phases.
Need even more resolution?
Choose 3 micron ACE columns
With todays increased pace of drug discovery, fast and
efficient methods are the rule. Short, narrow-bore columns
are replacing the conventional 250 x 4.6mm versions. ACE

HPLC columns are available in 3m, 5m and 10m
particle sizes. Although 5m particles are sufficient for most
applications, greater efficiency can be obtained by using
smaller particles. This increased efficiency enables the use of
shorter (<150mm) column lengths, resulting in decreased
analysis times. Due to the excellent flow characteristics of
ACE silica, you will not experience the high back pressures
often encountered with other columns.
Narrow-bore, Rapid analysis, LC/MS and Preparative
Columns
ACE bonded phases are available in a range of particle
sizes, so regardless of your application, you can scale up or
scale down and be assured of the same selectivity. For fast,
high resolution preparative chromatography, a range of
pre-packed ACE preparative and combinatorial chemistry
columns are available.
Analysis of Biomolecules
Choose wide pore 300 ACE columns
When molecular weight > 5000 it is generally recommended
to use wide pore materials in preference to small pore (ie.
100) materials, although molecular shape and structure
can affect this boundary.
The same ultra pure silica as used with the ACE 100
range is available in a 300 pore size for the analysis of
proteins, peptides and other biomolecules. A range of
bonded phases and particles sizes are available to enable
complete assay optimization.
Application Summary
Phase
Description
Application
C18
Optimized for maximum efficiency, superior peak shape and

resolution. Utilizes the same ultra high purity silica as all
ACE phases.
A C18 phase for most HPLC applications. Available in a range of particle

sizes, from LC/MS and microbore applications through to preparative scale
separations.
C8
Increased bonding density compared to ACE C18. Similarly optimized

for maximum efficiency, superior peak shape and resolution.
Recommended starting point for method development. Also suited to high

aqueous conditions and for rapid analysis applications.
C4
Combines lower hydrophobicity with excellent chromatographic

performance. Improved hydrolytic stability compared to conventional
C4 phases.
Use for rapid analysis optimization, when less retention than C8

or C18 is required. Also suitable for analysis of small proteins.
CN
Suitable for use in both normal- and reversed-phase modes. Greatly

improved performance, stability and reproducibility compared to
conventional CN phases.
Use to increase retention of polar compounds. Ideal for

gradients and rapid screening applications due to fast equilibration
capabilities.
Ph
Hydrophobicity between C4 and C8 phases, with increased

polar selectivity. Improved performance, stability and reproducibility
compared to conventional phenyl phases.
Offers alternative selectivity for aromatic, amine or polar compounds.
AQ
A unique C18 bonded phase with integral polar functionality. Resistant

to phase collapse even with 100% aqueous mobile phase.
Recommended for applications where 100% aqueous mobile phases are

required. Ideal for fast gradients due to rapid re-equilibration properties.
C18-HL
High surface area, high carbon load phase, leading to increased

retention and loading compared to ACE C18.
Suitable for LC/MS due to increased retention characteristics. Availability of

particle sizes up to 15m ensures easy scale-up for preparative and process
scale applications.
300
The same ultra inert, ultra pure silica as the ACE 100 columns is now
available in a wide pore 300 format. A range of bonded phases and
particle sizes are available.
Analysis of proteins, peptides and other biomolecules. The ultra high purity
300 silica provides improved peak shapes, especially at very low TFA
concentrations which in turn provides increased sensitivity.
ACE Application Guide
21
Application Index
Acacia farnesiana - ethanol extract from seed cover
Additives and intense sweeteners
Amino acids and biogenic amines in wine and beer
Amino acids derivatized with dabsyl chloride
Angiotensins
Annatto
Antihistamines and expectorants
Antihistamines and expectorants - bonded phase effects
Antihistamines and expectorants - mobile phase effects
Antihistamines and expectorants - rapid analysis
Artemisinin
Artificial food colors
Avenacins
Benzo(a)pyrene-7,8-quinone derived deoxynucleotide
DNA adducts
Beta blockers
Brompheniramine maleate
Catechins
Catecholamines
Catecholamines from plasma
Catecholamines from urine
Cefquinome
Clausena excavate - semi-preparative HPLC of
methanolic leaf extract
Clopidogrel
Cold medicine components
Cyclosporin mixture
Didanosine
Doxepin (cis and trans isomers)
Entacapone
Epanolol
Epinastine
Flavone and dibucaine - effect of temperature
Flavonoids
Flurbiprofen and related substances
Galanthamine
Garlic analysis 1
Garlic analysis 2
Herbicide - benfluralin
Herbicide - trifluralin
Hippuric acid
Ibuprofen and related impurities
Illegal dyes in spices
Insulins
Isoflavones
Local anesthetics
Malachite green
Maleic and fumaric acids
Nitroanilines
Nitrofuran metabolites
Ochratoxin A
Organic acids
Organotin compounds
OTC gastric drugs
Oxymetazoline in nasal spray formulation
22
Analyte Index
25
25
26
25
27
27
27
27
28
28
28
28
29
29
29
29
30
30
30
31
31
31
31
32
32
32
32
33
33
33
34
34
34
34
35
35
35
35
37
36
36
37
37
37
38
38
38
39
40
40
41
40
41
Paclitaxel
Paroxetine and desfluoro analogue
Peptide test mix
Peptides - selectivity changes with bonded phase and
mobile phase
Peptides - varying mobile phase
Peptides - varying pH
Pesticides in water
Phenolic acids
Phenols in purple coneflower (echinacea purpurea)
Pilocarpine
Polyamines
Polyethylene glycol 1000
Preservatives
Prostaglandins
Protein test mix
Proton pump inhibitors (PPIs)
PPI - Lansoprazole degradation studies
PPI - Lansoprazole degradation studies - bonded phase effects
Selectivity test mix
Selectivity test mix - high throughput analysis
Sotalol
Statins - atorvastatin
Statins - fluvastatin
Statins - pravastatin
Statins - simvastatin
Statin mixture
Substituted naphthalenes
Telithromycin
Terfenadine and fexofenadine in rat plasma
Test mix - Dolan 1
Test mix - Dolan 2
Test mix - Engelhardt
Test mix - Euerby
Test mix - Mutton
Testosterone
Tetracyclines
Tocopherols
Tricylic antidepressants
Tryptic digest of BSA
Vitamins - fat soluble
Vitamins - water soluble (isocratic)
Vitamins - water soluble (gradient)
Vitamins - water soluble (phase selectivity)
Vitamins - water soluble (rapid analysis)
Water soluble artificial colors
Whey proteins from whole milk
41
41
44
42
43
43
44
44
45
45
45
45
46
46
46
46
47
47
48
48
48
48
49
49
49
49
50
50
50
50
51
51
51
51
52
52
52
53
52
56
53
54
55
55
56
56
Acesulfame K
25
Acetaminophen
32
Acetophenone
50
4-Acetyl-2-fluorobiphenyl
34
n-Acetylprocainamide
51
Acetylsalicylic acid (aspirin)
32, 40
Adrenaline (epinephrine)
30, 31
Agmatine
26
Ajoene
35
Alachlor
44
Alpha-Alanine
26
Beta-Alanine
26
Allicin
35
Alliin
35
Allura red
56
Amaranth
28, 56
L-2-Aminoadipic acid
26
p-Aminobenzoic acid
54, 55
Amitriptyline
51, 53
Ammonium ion
25, 26
Angiotensin I
27
Angiotensin II
27, 42, 43, 44
Angiotensin III
27
Aniline
51
Anisole
50
Apomyoglobin
46
Arginine
25, 26
Artemisinin
28
Ascorbic acid (vitamin C)
32, 40, 53, 54
Asparagine
26
Aspartame
25
Aspartic acid
26
Aspirin (acetylsalicylic acid)
32, 40
Atorvastatin
48, 49
Atrazine
44
Avenacin A-1
29
Avenacin A-2
29
Avenacin B-1
29
Avenacin B-2
29
Benfluralin
35
Benzalkonium chloride
41
Benzoic acid
40, 46
Benzonitrile
51
Benzophenone
36
Benzyl alcohol
51
Benzylamine
51
d-Biotin (vitamin H)
54, 55
9-cis-Bixin
27
9-trans-Bixin
27
Brompheniramine maleate
29
BSA (tryptic digest)
52
Butter yellow
36
n-Butylbenzene
50
4-Butylbenzoic acid
48, 51
Cadaverine
26, 45
Caffeic acid
Caffeine
Caftaric acid
Carmoisine
Cefquinome
cis-Chalcone
trans-Chalcone
4-Chlorocinnamic acid
Chlorogenic acid
Chlorpheniramine maleate
Chlortetracycline
Cichoric acid
Cimetidine
Clopidogrel
Cocaine
p-Coumaric acid
Cyanazine
Cyanocobalamin (vitamin B12)
Cyclamate
Cyclosporin A
Cyclosporin B
Cyclosporin C
Cyclosporin D
Cyclosporin E
Cyclosporin G
Cyclosporin L
Cyclosporin U
Cysteine
Cytochrome C
Daidzein
Daidzin
Demeclocycline
Deoxyalliin
Desethylatrazine
Desfluoroparoxetine
Desisopropylatrazine
Dextromethorphan
Dibucaine
Dibutyltin
Didanosine
Diethylacetamide
Dihydrocyclosporin A
2,3-Dihydroxynaphthalene
3,4-Dihydroxynorephedrine
N,N-Dimethylaniline
1,4-Dimethylnaphthalene
Dimethyl phthalate
Diphenyl ether
L-DOPA
Dopamine
cis-Doxepin
trans-Doxepin
Doxycycline
Entacapone
Epanolol
44
25, 32
45
56
31
51
51
51
45
27, 28
52
45
40
31
37
44
44
44, 45
25
32
32
32
32
32
32
32
32
26
46
37
37
52
35
44
41
44
27, 28
34
41
32
50
32
51
30, 31
48, 51
50
48
51
30
30, 31
32
32
52
33
33
(+)-Epicatechin
Epigallocatechin
Epigallocatechin gallate
Epinastine
Erythrosine
Ethenzamide
Ethylbenzene
Ethyl benzoate
1-Ethylnaphthalene
2-Ethylnaphthalene
Ethyl paraben
Famotidine
Ferulic acid
Fexofenadine
Flavone
Flurbiprofen
Fluvastatin
Folic acid (vitamin M)
Fumaric acid
Furaltadone
Furazolidone
GABA
Galanthamine
Genistein
Genistin
Glutamic acid
Glutamine
Glycine
Gly-Tyr
Guaiacol
Guaifenesin
Hippuric acid
Histamine
Histidine
Holo-transferrin
p-Hydroxybenzoic acid
Hydroxyproline
Ibuprofen
Imipramine
Insulin (bovine)
Insulin (human)
Insulin (porcine)
Isoamylamine
Isocyclosporin A
Isoleucine
Isopilocarpine
Kaempferol
Alpha-Lactalbumin
Beta-Lactoglobulin A
Beta-Lactoglobulin B
Lansoprazole
Leucine
Leucine enkephalin
Leucomalachite green
Lignocaine
30
30
30
33
56
32
50, 51
51
50
50
46
40
44
50
34
34
49
54, 55
38
39
39
26
34
37
37
26
26
26
42, 43, 44
27, 28
27, 28
37
26
26
46
46
26
36
53
37
37
37
26
32
25, 26
45
34
56
56
56
46, 47
25, 26
42, 43
38
37
23
Analyte Index (continued)

Lysine
25, 26
Malachite green
38
Maleic acid
27, 28, 29, 38, 40
Metanil yellow
36
Methionine
25, 26
Methionine enkephalin
42, 43
2-Methylhippuric acid
37
2-Methylnaphthalene
50
Methyl paraben
46
Metoprolol
29
Neohesperidin dihydrochalcone
25
Neurotensin
44
Niacinamide (vitamin B3)
53, 54
Nicotine
51
Nicotinic acid
53, 54
m-Nitroaniline
38
o-Nitroaniline
38
p-Nitroaniline
38
4-Nitrobenzoic acid
51
Nitrofurazone
39
Noradrenaline (norepinephrine)
30, 31
Norephedrine
27, 53
Norbixin
27
Norleucine
25
Nortriptyline
53
Ochratoxin A
40
Omeprazole
46
Orange II
36
Ornithine
26
Oxymetazoline
41
Oxprenolol
29
Oxytetracycline
52
Oxytocin
44
Paclitaxel (taxol)
41
Pantothenic acid (vitamin B5)
54, 55
Para red
36
Paroxetine
41
Pendimethalin
44
Phenol
48, 51
Phenylalanine.
26
Phenylethylamine
26
Phthalic acid
46
Pilocarpine
45
Pindolol
29
Polyethylene glycol
45
Ponceau 4R
28, 56
Pravastatin
49
Procainamide
51
Procaine
37
Proline
25, 26
Prometryn
44
Propazine
44
Propranolol
29
Propyl paraben
46
46
Prostaglandin F2a
24
Acacia Farnesiana Ethanol Extract from Seed Cover

8-iso-Prostaglandin F2a
Putrescine
Pyridine
Pyridoxal
Pyridoxamine
Pyridoxine
Quercetin
Quercitrin
Quinine sulphate
Ranitidine
Red 2G
Rhodamine B
Riboflavin (vitamin B2)
Ribonuclease A
Rutin
Saccharin
Salbutamol
Salicylamide
Salicylic acid
Sebuthylazine
Serine
Sesamol
Simazine
Simvastatin
Sorbic acid
Sotalol
Spermidine
Sucralose
Sudan I
Sudan II
Sudan III
Sudan IV
Sudan black
Sudan orange G
Sudan red B
Sudan red 7B
Sudan red G
Sunset yellow
Tartrazine
Taxol (paclitaxel)
Telithromycin
Terbutaline
Terbuthylazine
Terbutryn
Terfenadine
Testosterone
Tetracycline
Theobromine
Theophylline
Thiamine (vitamin B1)
Thioctic acid
Thiourea
Threonine
Alpha-Tocopherol
Beta-Tocopherol
46
26, 45
48, 51
53, 54
53, 54
53, 54, 55
34
34
25
40
56
36
54, 55
46
34
25
51
27, 28
40
44
26
41
44
49
46
48
26
25
36
36
36
36
36
36
36
36
36
28, 56
28
41
50
51
44
44
50
52
52
25
25
53, 54
54
51
25, 26
52
52
Gamma-Tocopherol
52
Delta-Tocopherol
52
Toluene
48, 51, 53
Toluidine
51
Toluidine red
36
Tributyltin
41
Trifluralin
35
Tryptamine
26
Tryptophan
25, 26
Tyramine
26
Tyrosine
26, 30
Uracil
48
Valine
25, 26
Val-Tyr-Val
42, 43
Vitamin A
56
Vitamin A acetate
56
Vitamin B1 (thiamine)
53, 54
Vitamin B2 (riboflavin)
54, 55
Vitamin B3 (niacinamide)
53, 54
Vitamin B5 (pantothenic acid)
54, 55
Vitamin B12 (cyanocobalamin)
54, 55
Vitamin C (ascorbic acid)
32, 40, 53, 54
Vitamin D2
56
Vitamin D3
56
Vitamin E
56
Vitamin E acetate
56
Vitamin H (d-biotin)
54, 55
Vitamin K1
56
Vitamin K3
56
Vitamin M (folic acid)
54, 55
VMA (vanillylmandelic acid)
30
Additives and Intense Sweeteners

Conditions
Analytical method development for subsequent scale-up to

preparative HPLC
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
A: MeOH
B: H2O
2.0 ml/min
T(mins)
0
2.5
%A
15
15
%B
85
85
Ambient
UV, 230 nm
Column:
ACE 5 C18, 250 x 4.0mm
Part Number:
ACE-121-2504
Compounds
1. Acesulfame K
2. Theobromine
3. Theophylline
4. Cyclamate
5. Saccharin
6. Caffeine
7. Sucralose
8. Quinine sulphate
9. Aspartame
10. Neohesperidin
dihydrochalcone
Mobile Phase: A: H2O

B: MeCN

C: 1% TFA
60
50
50
62.5
50
50
70
15
85
Gradient:

T(mins)
0
25
30
35
Flow Rate:
1.0ml/min
%A
88
50
30
88
%B
2
40
60
2
%C
10
10
10
10
Temperature: 30C
Detection:
ELSD
Time - Minutes
Time - Minutes
Reproduced with kind permission of School of Pharmacy, University of Sunderland, UK
Reproduced with kind permission of Durham County Council, UK
Amino Acids Derivatized with Dabsyl Chloride

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Injection Volume:
ACE 3 C18, 150 x 3.0mm

ACE-111-1503
A. 10mM KH2PO4 buffer (pH 6.55)
B: 70:30 MeCN/2-Propanol
0.5ml/min
T(mins)
%A
%B
0
90
10
3
82
18
17
82
18
27
78
22
35
78
22
50
65
35
57
65
35
70
35
65
70.1
35
65
89
90
10
90
90
10
50C
UV, 436nm (PDA detector)
20l
Compounds
1. L-Arginine
5. L-Methionine
9. L-Leucine
2. L-Threonine
6. L-Isoleucine
10. Ammonium ion
3. L-Proline
7. L-Tryptophan
11. L-Lysine
4. L-Valine
8. L-Norleucine
11
4
6
7
1
9
8
10
Retention Time (min)
Reproduced with kind permission of Department of Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
25
Amino Acids and Biogenic Amines in Wine and Beer
Angiotensins
Annatto
Conditions
Compounds
Sample Preparation:

Derivatisation with diethyl

ethoxymethylmalonate
Column:
ACE 5 C18-HL, 250 x 4.6mm
Part Number:
ACE-321-2546
Mobile Phase:

A. 25mM acetate buffer (pH 5.8)

B: 80:20 MeCN/MeOH
Flow Rate:
0.8ml/min
Gradient:

T(mins)
0
20
30.5
33.5
65
73
78
82
85
Temperature:
16C
Detection:
DAD, 280, 269 and 300nm
Injection Volume:
20l
%A
55
40
83
83
60
28
18
0
0
1. Aspartic acid
2. Glutamic acid
I. S. ( L-2-Aminoadipic acid)
3. Asparagine
4. Serine
5. Hydroxyproline
6. Glutamine
7. Histidine
8. Glycine
9. Threonine
10. beta-Alanine
11. Arginine
12. alpha-Alanine
13. GABA
14. Proline
15. Histamine
16. Tyrosine
17. Ammonium ion
%B
45
60
17
17
40
72
82
100
100
18. Agmatine
19. Valine
20. Methionine
21. Cysteine
22. Isoleucine
23. Tryptophan
24. Leucine
25. Phenylalanine
26. Ornithine
27. Lysine
28. Spermidine
29. Tyramine
30. Putrescine
31. Tryptamine
32. Cadaverine
33. Phenylethylamine
34. Isoamylamine
Compounds
Column:
ACE 5 C18-300, 250 x 4.6mm
Part Number:
ACE-221-2546
1. Angiotensin II
2. Angiotensin III
3. Angiotensin I

B. 80:20 MeCN/0.1% TFA in H2O
Flow Rate:
1.0ml/min
Gradient:

T (mins)
0
15
%A
75
60
%B
25
40
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Detection:
UV, 215nm
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
70:30 MeCN/0.16%
CH3CO2H in H2O
1.2ml/min
Ambient
UV/VIS, 478nm
Compounds
1. Norbixin
2. 9-trans-Bixin
3. 9-cis-Bixin
Conditions
Reproduced with kind permission of Lincolne Sutton & Wood, Norwich, UK
Antihistamines and Expectorants - Bonded Phase Effects

Conditions
Antihistamines and Expectorants

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
50:50 MeOH/50mM
KH2PO4 (pH 3.0)
1.0ml/min
22C
UV, 220nm
Compounds
1. Maleic acid
2. Norephedrine
3. Salicylamide
4. Guaifenesin
5. Guaiacol
6. Chlorpheniramine
maleate
7. Dextromethorphan
Column:
ACE 5 C18 and ACE 5 Phenyl
Column Dimensions:
150 x 4.6mm
Part Numbers:
ACE-121-1546 and ACE-125-1546
Mobile Phase:
45:55 MeCN/20mM KH2PO4 (pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 220nm
Injection Volume:
0.4l
Compounds:

1. Maleic acid
2. Salicylamide
3. Guaifenesin
4. Guaiacol
5. Chlorpheniramine maleate
6. Dextromethorphan
ACE 5 C18
ACE 5 Phenyl
7
6
Time - Minutes
34
Reproduced with kind permission of Instituto de la Vid y el Vino de Castilla-La Mancha, Spain
26
27
Antihistamines and Expectorants - Mobile Phase Effects

Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number: ACE-121-1546
Mobile Phase: (a) 45:55 MeOH/50mM
KH2PO4 (pH 3.0)

(b) 28:72 MeCN/50mM
KH2PO4 (pH 3.0)
Flow Rate:
1.0ml/min
Detection:
UV, 220nm

Column:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Injection Volume:
Compounds
1. Maleic acid
2. Salicylamide
3. Guaifenesin
4. Guaiacol
5. Chlorpheniramine
maleate
6. Dextromethorphan
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Benzo(a)pyrene-7,8-quinone Derived Deoxynucleotide

DNA Adducts
ACE 5 C18, 250 x 4.6mm
ACE-121-2546
70:30 MeOH/H2O
1.0ml/min
Ambient
UV, 225nm
Partially purified extract
from oat root
(a) ACE 5 C18, 150 x4.6 mm

2
1
3
BPQ-dG #3 and BPQ-dG #4

(retention times: 25.3 and 26.1 mins)
Compounds
1. BPQ-dG#1
2. BPQ-dG#2
3. BPQ-dG#3
4. BPQ-dG#4
BPQ-dG #1 and BPQ-dG #2

(retention times: 14.7 and 15.8 mins)
Conditions
Column:
ACE 3 C18, 150 x 4.6mm
Part Number:
ACE-111-1546
Mobile Phase:
A. 0.1% HCO2H

B: MeCN
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A
%B

0
75
25

8
45
55
Temperature:
35C
Detection:
UV, 285nm
Injection Volume: 5l
5. Chlorpheniramine maleate
6. Dextromethorphan
Compounds
1. Avenacin A-2
2. Avenacin B-2
3. Avenacin A-1
4. Avenacin B-1
(b) Acetonitrile based mobile phase
(b) ACE 3 C18, 50 x4.6 mm
4
5
3 4
Avenacins
Conditions (b)
ACE 3 C18, 50 x 4.6mm
39:61 MeOH/20mM
KH2PO4 (pH 3.0)
2.0ml/min
Ambient
UV, 220nm
0.2l
Conditions (a)
ACE 5 C18, 150 x 4.6mm
45:55 MeOH/20mM
KH2PO4 (pH 3.0)
1.0ml/min
Ambient
UV, 220nm
0.4l
Compounds
1. Maleic acid
3. Guaifenesin
2. Salicylamide 4. Guaiacol
(a) Methanol based mobile phase
Antihistamines and Expectorants - Rapid Analysis
Time - Minutes
10
15
Artificial Food Colors
Artemisinin
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Gradient:

Temperature:
Detection:
Injection Volume:
25
30
Reproduced with kind permission of Institute of Cancer Research, Sutton, Surrey, UK
Brompheniramine Maleate
Beta Blockers
Conditions
Compounds
Column:
ACE 3 C18, 100 x 4.6mm
1. Tartrazine
2. Amaranth
3. Sunset Yellow
Mobile Phase: A. 3.1mM TBAB1 and 5mM
4. Ponceau 4R

KH2PO4 in H2O

B: 5mM KH2PO4 in MeOH
Flow Rate:
0.8ml/min
Gradient:
T(mins) %A
%B
Curve

0
55
45

12
40
60
6

25
55
45
1
Detection:
UV/VIS, 480nm
1
Tetrabutylammonium bromide
Compounds
1. Artemisinin
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
A: H2O B: MeOH
1.0ml/min
T(mins) %A
%B
0
50
50
25
0
100
35
0
100
20C
ELSD
20l
Reproduced with kind permission of Sainsbury Laboratory, John Innes Centre, UK
20
Time Minutes
Conditions
Compounds
1. Pindolol
2. Metoprolol
3. Oxprenolol
4. Propranolol
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Part Number:
ACE-121-2546
Mobile Phase:

65:35 MeOH/50mM
1-methylpiperidine
(pH 11.0)
Flow Rate:
1.0ml/min
Flow Rate:
Temperature:
Ambient
Temperature:
Ambient
Detection:
UV, 215nm
Detection:
UV, 265nm
Column:
ACE 5 CN, 150 x 4.6mm
Part Number:
ACE-124-1546
Mobile Phase:

5:95 MeOH/H2O
containing 20mM HCO2NH4,
pH 3.0 with HCO2H
Compounds
1. Maleic acid
2. Brompheniramine
maleate
1.0ml/min
Injection Volume: 20l (0.2mg/ml solution)
Brompheniramine
2
1
2
Reproduced with kind permission of Neem Biotech, Cardiff, UK
28
Time - Minutes
29
Catechins
Catecholamines
Conditions
Columns:
ACE 5 C18, ACE 5 Phenyl, ACE 5 CN
Column Dimensions: 150 x 4.6mm
Mobile Phase:
25:75 MeOH/0.1% HCO2H
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 280nm
Compounds
1. Epigallocatechin
2. (+)-Epicatechin
3. Epigallocatechin
gallate
ACE 5 C18
Catecholamines from Urine
Conditions
Column:
ACE 5 AQ, 150 x 4.6mm
Part number:
ACE-126-1546
Mobile Phase:
(a) 50mM KH2PO4, (pH 3.0)

(b) 50mM KH2PO4, (pH 2.0)

(c) 0.1% TFA

(d) 0.1% HCO2H
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 210nm
Compounds
1. Noradrenaline
2. Adrenaline
3. L-DOPA
4. Dopamine
5. L-Tyrosine
6. VMA
(vanillylmandelic acid)
Cefquinome
Conditions
Sample Preparation: Ion pair extraction using diphenyl-borate-ethanolamine.
Derivatisation using diphenyl-ethylenediamine as fluorescent probe
Column:
ACE 5 C18, 150 x 4.6mm
Compounds
1. N
oradrenaline
(norepinephrine)
Mobile Phase: 50:35:15 50mM CH3CO2Na

buffer (pH 7.0)/MeCN/MeOH 2. 3
,4-Dihydroxynorephedrine
(I.S.)
Flow Rate:
0.9ml/min
3. Adrenaline (epinephrine)
4. Dopamine
Detection:
Fluorescence - ex 350nm

- em 480nm
Noradrenaline
Adrenaline
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Compounds
1. Cefquinome
2. Excipient
ACE 5 C18, 150 x 2.1mm

ACE-121-1502
A: 2mM HCO2H in H2O
B: 2mM HCO2H in MeCN
0.2ml/min
Time %A
%B
0
95
5
1
95
5
10
5
95
30
5
95
25C
ESI-MS (+)
Cefquinome
(a) 50mM KH2PO4, (pH 3.0)
ACE 5 Phenyl
Dopamine
(b) 50mM KH2PO4, (pH 2.0)
ACE 5 CN
Time - Minutes
Reproduced with kind permission of MAS University Hospital, Malmo, Sweden
Clausena Excavate - Semi-preparative HPLC of Methanolic

Leaf Extract
6
5
Catecholamines from Plasma
(c) 0.1% TFA
Conditions
Sample Preparation: Ion pair extraction using diphenyl-borate-ethanolamine.
Derivatisation using diphenyl-ethylenediamine as fluorescent probe
Column:
ACE 5 C18, 150 x 4.6mm
Compounds
1. Noradrenaline
(norepinephrine)
Mobile Phase: 50:35:15 50mM CH3CO2Na

buffer (pH 7.0)/MeCN/MeOH 2. 3,4-Dihydroxynorephedrine
(I.S.)
Flow Rate:
0.9ml/min
3. Adrenaline (epinephrine)
10
15
25
30
While it is possible to scale up from analytical (4.6mm ID) to preparative

(21.2mm ID) dimensions, in this case it was convenient to carry out both
the method development and the actual isolation of fractions on the semipreparative column to yield enough material for testing and identification.
Conditions
Column:
ACE 5 C18, 250 x 7.75mm
Part Number:
ACE-121-2508
Mobile Phase:
20:80 0.5% TFA in MeOH/0.5% TFA in H2O
Flow Rate:
4.5ml/min
Temperature:
Ambient
Detection:
UV, 245nm
Reproduced with kind permission of Department of Soil Science, University of Reading, UK
Clopidogrel
Analysis of clopidogrel and photodegradation products after 3.5 hours
exposure to UV light
Conditions
Compounds
Column:
ACE 5 C18, 150 x 4.6mm
1. Clopidogrel
Part Number:
ACE-121-1546
Mobile Phase:
75:25 MeOH/aqueous TEA

(pH 5.3 with H3PO4)
Flow Rate:
1.2ml/min
Temperature:
25C
Detection:
UV, 220nm
Clopidogrel
Detection:
Fluorescence - ex 350nm

- em 480nm
20
Time - Minutes
Noradrenaline
(d) 0.1% HCO2H
Adrenaline
Time - Minutes
Time - Minutes
Reproduced with kind permission of MAS University Hospital, Malmo, Sweden
30
Time - Minutes
Reproduced with kind permission of Brazilian Pharmacopoeia
31
Cold Medicine Components
Cyclosporin Mixture
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Mobile Phase: 50:50 MeOH/50mM KH2PO4

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature: 22C
Detection:
UV, 220nm
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Compounds
1. Vitamin C
2. Acetaminophen
3. Caffeine
4. Aspirin
5. Ethenzamide
Entacapone
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Injection Volume:
Analysis of entacapone standard in methanol solution after exposure to

direct UV radiation (254nm).
Conditions
Compounds
Column:
ACE 5 C18, 250 x 4.6mm
1. Degradation product
Part Number:
ACE-121-2546
2. Entacapone
Mobile Phase:
35:65 MeCN/aqueous

H3PO4 (pH 3.0)
Flow Rate:
2.0ml/min
Temperature:
25C
Detection:
UV, 305nm
Compounds
1. Isocyclosporin A
2. Cyclosporin C
3. Cyclosporin B
4. Cyclosporin L
5. Cyclosporin U
6. Cyclosporin A
7. Dihydrocyclosporin A
8. Cyclosporin G
9. Cyclosporin D
10.Cyclosporin E
ACE 5 C18, 250 x 3.0mm

ACE-121-2503
46:51:3:0.1
H2O/MeCN/MTBE/H3PO4
0.8ml/min
80C
UV, 210nm
Epanolol
ACE 5 CN, 150 x 4.6mm
ACE-124-1546
15:85 MeOH/H2O containing
20mM HCO2NH4, pH 3.0
with HCO2H
1.0ml/min
Ambient
UV, 254nm
20l (0.2mg/ml solution)
Entacapone
1
2
2
60
1
3
7 8
10
60
50
50
40
40
30
30
mAU
20
mAU
Compounds
1. Epanolol
20
Reproduced with kind permission of Camurus AB, Lund, Sweden
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Compounds
1. Didanosine
Time - Minutes
Time - Minutes
Epinastine
Compounds
1. cis-Doxepin
2. trans-Doxepin
ACE 5 SIL, 250 x 4.6mm

ACE-127-2546
95:5:0.3 Hexane/ethanol/TEA
1.0ml/min
Ambient
UV, 254nm
Development and validation of HPLC and UV derivate spectrophotometric

methods for determination of epinastine hydrochloride in coated tablets
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:
60:40 0.3% aqueous TEA (pH 4.0 with H3PO4)/MeOH
Flow Rate:
1.0ml/min
Temperature:
25C
Detection:
UV, 254nm
Compounds
1. Epinastine
Fig. 1 Epinastine Hydrochloride reference substance (A) before and (B) after 18
hours in a UV254nm chamber
Fig. 2 Magnified version of Fig. 1
Time - Minutes
Reproduced with kind permission of Aptuit Ltd, Edinburgh, UK
32
Doxepin (cis and trans isomers)

ACE 5 C18-HL, 250 x 4.6mm
ACE-321-2546
20:80 MeOH/50mM
CH3COONH4, pH 8.0
1.5ml/min
Ambient
UV, 254nm
10
Time - Minutes
Didanosine
10
Time - Minutes
33
Flavone and Dibucaine - Effect of Temperature

Conditions
Column:
ACE 3 C18, 30 x 4.6mm
Mobile Phase: A: 6.5mM CH3CO2NH4

B: MeCN

C: MeOH
Flow Rate:
2.0ml/min
Gradient:
Time %A
%B %C

0
80
10
10

5.2
0
50
50

5.6
0
0
100
Temperature: 40C and 60C
Detection:
DAD, 200-450nm
Flavonoids
Garlic Analysis 1
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Compounds
1. Flavone
2. Dibucaine
Flavone
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Part Number:
ACE-121-2546
Mobile Phase:
50:50 MeOH/H2O
Flow Rate:
1.0ml/min
Temperature:
30C
Detection:
UV, 210nm
Compounds
1. Rutin
2. Quercetin
3. Quercitrin
4. Kaempferol
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
40:60 MeCN/0.1% HCO2H
1.0ml/min
Ambient
UV, 254nm
1l
Garlic Analysis 2
Compounds
1. Allicin
2. Alliin
3. Deoxyalliin
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Injection Volume:
Dibucaine
60C
2, 3
1, 2
40C
Compounds
1. Allicin
2. Ajoene
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
A: H2O
B: MeCN
1.0ml/min
T(mins) %A
%B
0
60
40
20
0
100
25
0
100
30C
UV, 254nm
20l
Time - Minutes
Reproduced with kind permission of Johnson & Johnson Pharmaceutical Research & Early
Development, a division of Janssen-Cilag, S.A., Toledo, Spain
Herbicide - Trifluralin
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Flow Rate:
1.0ml/min
Detection:
UV, 254nm
Compounds
1. Galanthamine
Herbicide - Benfluralin
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Part Number:
ACE-121-2546
Mobile Phase:
8:92 MeCN/0.1% TFA
Flow Rate:
1.0ml/min
Temperature:
20C
Detection:
UV, 210nm
Compounds
1. 2-(2-Fluoro-4-biphenyl)-2hydroxypropionic acid
2. cis-2-(2-Fluoro-4-biphenyl)2-hydroxypropionic acid
3. 2-Fluoro-4-biphenyl-4carboxylic acid
4. Flurbiprofen
5. 4-Acetyl-2-fluorobiphenyl
Time
- Minutes
Galanthamine
Flurbiprofen and Related Substances

Conditions
Column:
ACE 3 C18, 50 x 4.6mm
Part Number:
ACE-111-0546
Mobile Phase:
34:64:0.5 MeCN/H2O/TFA
Flow Rate:
2.0ml/min
Temperature:
28C
Detection:
UV, 254nm
Injection Volume: 20l (1mg/ml solution)
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Compounds
1. Benfluralin
Compounds
1. Trifluralin
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
85:15 MeOH/H2O
1.0ml/min
Ambient
UV, 254nm
Flurbiprofen
Time - Minutes
34
Time - Minutes
35
Ibuprofen and Related Impurities

Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Hippuric Acid
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Compounds
1. 2-(4-Methylphenyl)propanoic acid (Impurity D)
2. 2-(4-Isobutylphenyl)propanamide (Impurity C)
3. Benzophenone (Internal Standard)
4. 2-(3-Isobutylphenyl)propanoic acid (Impurity A)
5. Ibuprofen
6. 2-(4-Butylphenyl)propanoic acid (Impurity B)
7. 1-(4-Isobutylphenyl)ethanone (Impurity E)
ACE 5 C18, 150 x 3.0mm

ACE-121-1503
36:64 0.1% TFA in MeCN/0.1% TFA in H2O
1.5ml/min
40C
UV, 214nm
Insulins
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Compounds
1. Hippuric acid
2. 2-Methylhippuric acid
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
85:15 10mM KH2PO4
(pH 3.5)/MeCN
1.0ml/min
Ambient
UV, 254nm
Compounds
1. Bovine insulin
2. Human insulin
3. Porcine insulin
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
A. 29:71 MeCN/H2O + 0.1% TFA
B. 32:68 MeCN/H2O + 0.1% TFA
1.0ml/min
T(mins)
%A
%B
0
90
10
16
10
90
Ambient
UV, 215nm
Ibuprofen
Impurity A
Impurity B
Impurity C
Impurity D
Impurity E
Reproduced with kind permission of Boots Healthcare International, Nottingham, UK
Illegal Dyes in Spices

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:

Injection Volume:
1
Local Anesthetics
Isoflavones
Compounds
1. Rhodamine B
2. Orange II
3. Metanil Yellow
4. Butter Yellow
5. Para Red
6. Sudan Orange G
7. Toluidine Red
8. Sudan I
ACE 3 C18, 100 x 4.6mm

ACE-111-1046
A: H2O
B: MeOH
C: 0.06M TBAB1 and 0.5M KH2PO4 in H2O, pH 2.55
1.0ml/min
T(mins) %A
%B
%C
Curve
0
45
50
5
45
3
92
5
6
65
3
92
5
11
66
45
50
5
1
75
45
50
5
1
Ambient
UV/VIS, 420nm, 520nm
and 600nm
10l
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:
35:65 MeCN/0.1% HCO2H
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
9. Sudan Red G
10. Sudan II
11. Sudan Black
12. Sudan III
13. Sudan Red 7B
14. Sudan Red B
15. Sudan IV
Conditions
Column:
ACE 5 AQ, 250 x 4.6mm
Mobile Phase: 21:79:0.1 MeCN/H2O/2.5M H2SO4
Flow Rate:
1.5 ml/min
Detection:
UV
Compounds
1. Daidzin
2. Genistin
3. Daidzein
4. Genistein
Compounds
1. Procaine
2. Lignocaine
3. Cocaine
7,8
1
5
10
11
1
3
13
14
15
12
9
Time - Minutes
Time - Minutes
36
Reproduced with kind permission of Forensic Science Laboratories, Lothian and Borders
Police, UK
37
Malachite Green
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Maleic and Fumaric Acids

Compounds
1. Malachite green
2. Leucomalachite green
ACE 5 C18, 150 x 3.0mm

ACE-121-1503
20:80 MeCN/10mM
oxalic acid (pH 2.9)
0.4ml/min
Ambient
UV/VIS, 618nm
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Nitrofuran metabolites by LC-MS/MS

Compounds
1. Fumaric acid
2. Maleic acid
ACE 5 AQ, 250 x 4.6mm

ACE-126-2546
50mM KH2PO4(pH 7.0)
1.0ml/min
Ambient
UV, 210nm
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Injection Volume:
ACE 3 C18, 50 x 2.1mm

ACE-111-0502
50:50 0.5mM CH3COONH4 / MeOH
0.2ml/min
Ambient
ESI (+) MS/MS
20l
Nitrofuran
Metabolite
Derivative
Parent Ion (m/z)
Furazolidone
3-amino-2-oxazolidinone (AOZ)
NBAOZ
236
236
134
Furaltadone
5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ)
NBAMOZ
335
335
291
Nitrofurazone
1-aminohydantoin (AHD)
NBAHD
249
249
134
Extracts were assayed by LC-MS/MS using electrospray ionization in the

positive ion mode. Figure 1 shows the MRM transitions used for quantitation
of each antibiotic. Figure 2 shows representative chromatograms for different
concentration calibration standards of NBAHD. Good limits of quantitation
and linearity of detector response (see Figure 3 ) were obtained.
Malachite green
Nitrofuran veterinary antibiotics are banned in many countries due to

potential carcinogenic risks to human health. These nitrofurans are rapidly
metabolized in tissue. As a result, the metabolites are used as markers for
detection of the parent drug in animal food products. The metabolites are
derivatized with 2-nitrobenzaldehyde to form nitrophenyl derivatives, prior
to LC-MS analysis.
MRM Transition
Figure 1. MRM Chromatograms
Figure 3. Standard calibration curves
335
236
Time - Minutes
134
291
NBAMOZ
NBAOZ
Reproduced with kind permission of Central Science Laboratory, York, UK
249
Nitroanilines
Figure 2. Typical MRM Chromatograms for NBAHD
B. ACE 5 Phenyl
Intensity
Intensity
2 3
A. ACE 5 C18
1 2
NBAHD
Compounds
1. p-Nitroaniline
2. m-Nitroaniline
3. o-Nitroaniline
Intensity
Conditions
Columns:
A. ACE 5 C18, B. ACE 5 Phenyl,

C. ACE 5 CN
Mobile Phase:

Columns A & B: 50:50 MeCN/50mM K2HPO4 (pH 3.15)

Column C:
90:10 Heptane/EtOAc
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
134
C. ACE 5 CN
3
3
Time - Minutes
Time - Minutes
Time - Minutes
Reproduced with kind permission of Agricultural Ministry, Brazil
38
39
Ochratoxin A
Organotin Compounds
OTC Gastric Drugs
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Mobile Phase: 51:47:2 MeCN/H2O/CH3CO2H
Flow Rate: 1.0ml/min
Detection:
Fluorescence - lex 333nm

lem 443nm
Compounds
1. Ochratoxin A
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Detection:
Compounds
1. Famotidine
2. Cimetidine
3. Ranitidine
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
18:82 MeCN/10mM NH4HCO3
(pH as specified)
1.0ml/min
Ambient
UV, 254nm
Famotidine
Oxymetazoline in Nasal Spray Formulation

Compounds
1. Dibutyltin
2. Tributyltin
ACE 3 C18, 150 x 2.1mm

ACE-111-1502
65:23:12:0.05
MeCN/H2O/CH3CO2H/TEA
0.2ml/min
ICP-MS
pH 7.0
pH 8.0
Time - Minutes
Reproduced with kind permission of R-Biopharm Rhone Ltd, Glasgow, UK
Benzalkonium chloride
Oxymetazoline
Compounds
1. Oxymetazoline
2. Benzalkonium chloride
Ranitidine
Cimetidine
Ochratoxin A
Conditions
Column:
ACE 5 CN, 150 x 4.6mm
Mobile Phase: 50:50 MeCN/ aqueous

Na2HPO4, pH 7.0
Flow Rate:
1.5ml/min
Temperature: 30C
Detection:
UV, 214nm
Reproduced with kind permission of LGC Ltd., Middlesex, UK
Reproduced with kind permission of Thornton & Ross Ltd, Huddersfield, UK
Organic Acids
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
30:70 MeOH/50mM
KH2PO4 (pH 5.7)
1.0ml/min
22C
UV, 220nm
Compounds
1. Ascorbic acid
2. Maleic acid
3. Acetylsalicylic acid
4. Benzoic acid
5. Salicylic acid
Paclitaxel
pH 8.5
Paroxetine and Desfluoro Analogue
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Compounds
1. Paclitaxel (taxol)
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
55:45 MeCN/ H2O
1.0ml/min
40C
UV, 227nm
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Injection Volume:
Sesamol
40
Paroxetine
pH 9.0
Desfluoroparoxetine
Compounds
1. Sesamol
2. Desfluoroparoxetine
3. Paroxetine
ACE 5 CN, 150 x 4.6mm

ACE-124-1546
40:60 MeOH/H2O
containing 20mM HCO2NH4,
pH 3.0
2.0ml/min
Ambient
UV, 295nm
Reproduced with kind permission of Biointeractions Ltd, Reading, UK
41
Peptides - Selectivity changes with bonded phase and mobile phase
Peptides - Varying Mobile Phase
Compounds
1. Gly-Tyr
2. Val-Tyr-Val
3. Methionine enkephalin
4. Angiotensin II
5. Leucine enkephalin
0.1% TFA
0.1% HCO2H
ACE 5 C18-300
ACE 5 C18-300
45
2
3
34
34
ACE 5 Phenyl-300
ACE 5 Phenyl-300
5
4
4 3
3,4
pH 4.4
0.02% CH3CO2H
4 5
ACE 5 CN-300
2
4
3
pH 6.5
0.02% 20mM KH2PO4, pH2.0
ACE 5 CN-300
3 4
2
3 45
42
0.02% HCO2H
34
4,5
2
ACE 5 C4-300
ACE 5 C4-300
45
pH 2.0
ACE 5 C8-300
ACE 5 C8-300
3,4
0.02% TFA
Compounds
1. Gly-Tyr
2. Val-Tyr-Val
4. Angiotensin II
Conditions
Column:
ACE 5 C18-300
Part Number:
ACE-221-2546
Mobile Phase:
A: 20mM KH2PO4,
pH as indicated

B: MeCN
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A %B

0
90
10

25
60
40
Temperature:
Ambient
Detection:
UV, 220nm
Compounds
1. Gly-Tyr
2. Val-Tyr-Val
4. Angiotensin II
Conditions
Column:
ACE 5 C18-300
Part Number:
ACE-221-2546
Mobile Phase:
A: Buffer as indicated

B: MeCN
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A
%B

0
90
10

25
60
40
Temperature:
Ambient
Detection:
UV, 220nm
Conditions
Columns:
ACE 5 C18-300, ACE 5 C8-300, ACE 5 C4-300,

ACE 5 Phenyl-300, ACE 5 CN-300
Mobile Phase:
A: 0.1% TFA or 0.1% HCO2H B: MeCN
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A
%B

0
90
10

25
60
40
Temperature:
Ambient
Detection:
UV, 220nm
Peptides - Varying pH
43
Peptide Test Mix

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Phenolic acids
Compounds
1. Gly-Tyr
2. Oxytocin
3. Angiotensin II
4. Neurotensin
ACE 5 C18-300, 250 x 4.6mm

ACE-221-2546
A. 0.1% TFA in H2O
B. 0.1% TFA in MeCN
1.0ml/min
T(mins)
%A
%B
0
90
10
25
60
40
Ambient
UV, 220nm
Phenols in Purple Coneflower (Echinacea Purpurea)
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:
20:80 MeCN/0.1% HCO2H
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Caffeic acid
p-Coumaric acid
Compounds
1. Caftaric acid
2. Chlorogenic acid
3. Cichoric acid
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Part Number:
ACE-121-2546
Mobile Phase:
A: 0.1% H3PO4 in H2O

B: MeCN
Flow Rate:
1.5ml/min
Gradient:
T(mins) %A
%B

0
90
10

13
78
22

14
60
40
Temperature:
35C
Detection:
UV, 330nm
Compounds
1. Caffeic acid
2. p-Coumaric acid
3. Ferulic acid
Ferulic acid
Pilocarpine
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Detection:
Compounds
1. Isopilocarpine
2. Pilocarpine
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
15:85 MeCN/2mM
tetrabutylammonium
dihydrogen phosphate
1.0ml/min
UV, 254nm
3
2
4
Time - Minutes
Reproduced with kind permission of Bionorica Extracts SL, Spain
Reproduced with kind permission of Controlled Therapeutics, Scotland, UK
Polyamines
Pesticides in Water
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:

Injection Volume:
Sample:

Compounds
1. Desisopropylatrazine
2. Desethylatrazine
3. Simazine
4. Cyanazine
5. Atrazine
6. Internal standard
7. Sebuthylazine
ACE 3 C18, 150 x 2.1mm

ACE-111-1502
A: 0.1M CH3COONH4
B: MeCN
0.3ml/min
T(mins) %A
%B
0
90
10
40
20
80
47
10
90
49
90
10
40C
UV, 220nm
(pendimethalin at 245nm)
25l
0.05g/l standards in
10:90 MeCN/H2O
Polyethylene Glycol 1000

Compounds
1. Putrescine
2. Cadaverine
(as OPA derivatives)
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Mobile Phase: 90:10 MeOH/TRIS buffer

(pH 7.0)
Flow Rate:
1.2ml/min
Detection:
Fluorescence - lex 340nm

lex 450nm
8. Propazine
9. Terbuthylazine
10. Prometryn
11. Terbutryn
12. Alachlor
13. Pendimethalin
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Detection:
ACE 3 C8, 150 x 4.6mm

ACE-112-1546
A. 0.1% HCO2H in H2O
B. MeOH
1.0ml/min
T(mins)
0
45
%A
50
15
%B
50
85
APCI (negative ion)
50
50
50
60
50
50
2
6
3
4
10
11
12
13
Reproduced with kind permission of Amt der Tiroler Landesregierung, Chemisch-technische Umweltschutzanstalt, Innsbruck, Austria
44
Time - Minutes
Reproduced with kind permission of Centre for Chemical Sciences, Royal Holloway
University of London, UK
Time - Minutes
Reproduced with kind permission of Wickham Laboratories, UK
45
Preservatives
Prostaglandins
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Compounds
1. Phthalic acid
2. p-Hydroxybenzoic acid
3. Benzoic acid
4. Sorbic acid
5. Methyl paraben
6. Ethyl paraben
7. Propyl paraben
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
40:60 MeCN/50mM
KH2PO4 (pH 4.4)
1.0ml/min
Ambient
UV, 230nm
PPI - Lansoprazole Degradation Studies
Conditions
Compounds
Column:
ACE 3 C18, 50 x 2.1mm
1. 8-iso-Prostaglandin F2a
2. Prostaglandin F2a
Part Number:
ACE-111-0502
Mobile Phase:
A: H2O B: MeOH C: MeCN
Flow Rate:
0.2ml/min
Gradient:
T(mins) %A %B
%C

0
70
20
10

9
10
60
30

10
0.1 66.6 33.3
Temperature:
40C
Detection:
ESI (-) MS/MS
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
33:67 MeCN/10mM HCO2NH4 (pH 3.0)
1.0ml/min
Ambient
UV, 254nm
Non-degraded Lansoprazole
PPI-Lansoprazole Degradation Studies-Bonded Phase Effects

Conditions
Column:

Column Dimensions:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

ACE 5 C18, ACE 5 C8, ACE 5 C4, ACE 5 CN,

ACE 5 Phenyl, ACE 5 AQ, ACE 5 C18-HL
150 x 4.6mm
1.0ml/min
Ambient
UV, 254nm
Degraded Lansoprazole
(24 hours with 0.4M NaOH)
ACE 5 C18
Lansoprazole
3
8-iso-Prostaglandin F2a
Lansoprazole
Prostaglandin F2a
MRM 353.3 >193
ACE 5 C8
Degraded Lansoprazole after 24 hours with 0.4M NaOH
Lansoprazole
Lansoprazole
ACE 5 C4
Time
- Minutes
Reproduced with kind permission of Department of Internal Medicine,

Fukuoka University, Japan
Protein Test Mix
Lansoprazole
Proton Pump Inhibitors (PPIs)

Compounds
1. Ribonuclease A
2. Cytochrome C
3. Holo-transferrin
4. Apomyoglobin
Conditions
Column:
ACE 5 C18-300, 250 x 4.6mm

B. 0.1% TFA in MeCN
Flow Rate:
1.0ml/min
Gradient:
T(mins)
%A
%B

0
95
5

30
30
70
Detection:
UV, 280nm
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
ACE 5 C18, 150 x 4.6mm

ACE-121-1546
1.0ml/min
Ambient
UV, 254nm
Compounds
1. Omeprazole
2. Lansoprazole
ACE 5 CN
Lansoprazole
Degraded Lansoprazole after 24 hours with 0.4M HCI
Lansoprazole
ACE 5 Phenyl
Lansoprazole
Omeprazole
Lansoprazole
ACE 5 AQ
Degraded Lansoprazole after 24 hours with 3% H2O2
Lansoprazole
Lansoprazole
ACE 5 C18-HL
Lansoprazole
46
Time - Minutes
47
Selectivity Test Mix

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Selectivity Test Mix - High Throughput Analysis
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
60:40 MeCN/50mM
KH2PO4 (pH 3.2)
1.0ml/min
Ambient
UV, 254nm
Compounds
1. Uracil
2. Pyridine
3. Phenol
5. N,N-Dimethylaniline
6. 4-Butylbenzoic acid
Conditions (a)
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
2. Phenol
Statins - Pravastatin
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:
53:47 MeCN/10mM HCO2NH4

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Conditions (b)
ACE 3 C18, 20 x 4.6mm
ACE-111-0246
56.5:43.5 MeCN/10mM
HCO2NH4 (pH 3.2)
2.0ml/min
23C
UV, 254nm
ACE 3 C18, 150 x 4.6mm

ACE-111-1546
60:40 MeCN/10mM
HCO2NH4 (pH 3.2)
1.0ml/min
23C
UV, 254nm
Compounds
1. Pyridine
4. N,N-Dimethylaniline
Statins - Fluvastatin
Compounds
1. Fluvastatin
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Compounds
1. Pravastatin
6. Toluene
(b) ACE 3 C18, 20 x 4.6mm
(a) ACE 3 C18, 150 x 4.6mm
2
1
Time
- Minutes
Time
- Minutes
Time - Minutes
Sotalol
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Injection Volume:
Statins - Atorvastatin
ACE 5 CN, 150 x 4.6mm
ACE-124-1546
15:85 MeOH/H2O containing
20mM HCO2NH4,
pH 3.0 with HCO2H
1.0ml/min
Ambient
UV, 254nm
Statins - Simvastatin
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Compounds
1. Sotalol
Compounds
1. Atorvastatin
Statin Mixture
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Conditions
Column:
ACE 5 C18, 150 x 4.6mm
Part Number:
ACE-121-1546
Mobile Phase:

(pH 3.0)
Flow Rate:
1.0ml/min
Temperature:
Ambient
Detection:
UV, 254nm
Compounds
1. Simvastatin
Compounds
1. Impurity
2. Pravastatin
3. Atorvastatin
4. Simvastatin
1
1
Time - Minutes
Time
- Minutes
Time
- Minutes
48
49
Substituted Naphthalenes
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Telithromycin
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
51:49 MeCN/H2O
1.5ml/min
18C
UV, 225nm
Compounds
1. 2-Methylnaphthalene
2. 1-Ethylnaphthalene
3. 1,4-Dimethylnaphthalene
4. 2-Ethylnaphthalene
5. n-Butylbenzene
1-Ethylnaphthalene
2-Methylnaphthalene
Test Mix - Dolan 2

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Analysis of telithromycin after 1 hour exposure to 3% H2O2

Conditions
Compounds
Column:
ACE 5 C18, 250 x 4.6mm
1. Telithromycin
2. Degradant 1
Part Number:
ACE-121-2546
3. Degradant 2
Mobile Phase:
55:45 MeOH/0.05M

phosphate buffer (pH 4.0)
Flow Rate:
1.0ml/min
Temperature:
50C
Detection:
UV, 265nm
Test Mix - Engelhardt

Compounds
1. Amitriptyline
2. Benzonitrile
4. cis-Chalcone
5. trans-Chalcone
ACE 3 C18, 150 x 4.6mm

ACE-111-1546
50:50 MeCN/60mM KH2PO4
(pH 2.8)
1.0ml/min
Ambient
UV, 215nm
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Compounds
1. Thiourea
2. Aniline
3. Phenol
4. Toluidine (o,m,p combined)
5. Dimethylaniline
6. Ethyl benzoate
7. Toluene
8. Ethylbenzene
ACE 3 C18, 150 x 4.6mm

ACE-111-1546
55:45 MeOH/H2O
1.0ml/min
Ambient
UV, 254nm
2-Ethylnaphthalene
1,4-Dimethylnaphthalene
Telithromycin
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
10
11
12
13
14
Terfenadine
Compounds
1. Diethylacetamide
2. Anisole
3. Acetophenone
4. Ethylbenzene
Compounds
1. Nicotine
2. Benzylamine
3. Procainamide
4. Terbutaline
5. Salbutamol
6. Phenol
7. Benzyl alcohol
Compounds
1. Pyridine
2. Benzylamine
3. n-Acetylprocainamide
4. Benzyl alcohol
5. Phenol
6. 4-Nitrobenzoic acid
7. 2,3-Dihydroxynaphthalene
8. 4-Chlorocinnamic acid
9. Diphenyl ether
Conditions
Column:
ACE 3 C18, 150 x 4.6mm
Mobile Phase: A: 0.1% H3PO4 in H2O

B: 0.1% H3PO4 in MeCN
Gradient:
T(mins) %A
%B

0
95
5

35
0
100
Detection:
UV, 215nm
436.3
Test Mix - Mutton
Conditions
Column:
ACE 3 C18, 150 x 4.6mm
Mobile Phase: 5:95 MeOH/20mM KH2PO4

(pH 2.7)
Flow Rate:
1.0ml/min
Detection:
UV, 210nm
tR=2.09 mins
m/z 472.3
15
466.3
Test Mix - Euerby
ACE 3 C18, 150 x 4.6mm

ACE-111-1546
50:50 MeCN/60mM KH2PO4
(pH 2.8)
1.0ml/min
Ambient
UV, 215nm
m/z 502.3
Test Mix - Dolan 1
Compounds
Conditions
1. Fexofenadine
Column:
ACE 5 AQ, 50 x 3.0mm
2. Terfenadine
Part Number:
ACE-126-0503
Mobile Phase:
A: 0.1% HCO2H

B: MeOH
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A
%B

0
90
10

1.5
10
90

2.0
10
90

3.0
90
10
Temperature:
Ambient
Detection:
Turbo IonSpray MS/MS (+ve)
466.3 for fexofenadine

m/z 502.3
436.3 for terfenadine

m/z 472.3
tR=2.00 mins
Terfenadine and Fexofenadine in Rat Plasma
Time - Minutes
Reproduced with kind permission of Department of Environmental Chemistry,

University of Glasgow, UK
Fexofenadine
5
1
4
2
9
5
Reproduced with kind permission of Aptuit, Edinburgh, UK
50
51
Testosterone
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Tetracyclines
Compounds
1. Testosterone
ACE 5 C18, 150 x 0.075mm

ACE-121-1500075
A: 10:90 0.1% HCO2H in
MeCN/0.1% HCO2H in H2O
B: 90:10 0.1% HCO2H in
MeCN/0.1% HCO2H in H2O
1.0l/min
Time
%A
%B
0
60
40
5
60
40
30
5
95
Ambient
ESI (+) MS/MS
Tricyclic Antidepressants Bonded Phase Effects
Conditions
Column:
ACE 5 C18, 150 x 3.0mm
Part Number:
ACE-121-1503
Mobile Phase:
20:80 MeCN/10mM

oxalic acid (pH 2.9)
Flow Rate:
0.5ml/min
Temperature:
Ambient
Detection:
UV/VIS, 350nm
Compounds
1. Oxytetracycline
2. Tetracycline
3. Demeclocycline
4. Chlortetracycline
5. Doxycycline
Conditions
Columns:

Column Dimensions:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Compounds
1. Norephedrine
2. Nortriptyline
3. Toluene
4. Imipramine
5. Amitriptyline
ACE 5 C18, ACE 5 C8, ACE 5 C4,

ACE 5 CN and ACE 5 Phenyl
250 x 4.6mm
80:20 MeOH/25mM
KH2PO4 (pH 6.0)
1.0ml/min
22C
UV, 215nm
Compounds
1. Pyridoxamine
2. Thiamine (Vitamin B1)
3. Ascorbic acid (Vitamin C)
4. Nicotinic acid
5. Pyridoxal
6. Impurity
7. Pyridoxine
Conditions
Column:
ACE 5 C18, 250 x 4.6mm
Mobile Phase: 3:97 MeOH/50mM

KH2PO4 (pH 3.0)
Flow Rate:
1.0ml/min
Detection:
UV, 205nm
ACE 5 C18
Time - Minutes
Vitamins Water Soluble (Isocratic)
5
6
ACE 5 C8
4
3
Time - Minutes
Reproduced with kind permission of University of London, UK
Time - Minutes
Reproduced with kind permission of Central Science Laboratory, York, UK
Tocopherols
Tryptic Digest of BSA
Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Compounds
1. gamma-Tocopherol
2. alpha-Tocopherol
3. beta-Tocopherol
4. beta-Tocopherol
5. delta-Tocopherol
ACE 5 SIL, 250 x 4.6mm

ACE-127-2546
98:2 Hexane/IPA
1.0ml/min
Ambient
UV/VIS, 450nm
1l
ACE 5 C4
4
5
Conditions
Column:
ACE 5 C18-300, 150 x 4.6mm
Mobile Phase: A. 1% TFA in H2O

B. 50:50 1% TFA in MeCN/H2O
Gradient:
T(mins) 0
5 25 45

%A
96 96 80 80

%B
4
4 20 20
Detection:
UV, 214nm
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
75
60
40
95
35
65
115
30
70
120
96
4
Vitamins Water Soluble (Isocratic)
Time - Minutes
ACE 5 C8, 250 x 4.6mm

ACE-122-2546
3:97 MeOH/50mM
KH2PO4 (pH 2.5)
1.0ml/min
Ambient
UV, 205nm
Compounds
1. Pyridoxamine
4. Niacinamide (Vitamin B3)
5. Pyridoxal
6. Nicotinic acid
7. Pyridoxine
ACE 5 CN
5
4
4 5
Time - Minutes
ACE 5 Phenyl
3
4
3
2
Time - Minutes
Reproduced with kind permission of Department of Food Biosciences,

University of Reading, UK
52
Time - Minutes
53
Vitamins Water Soluble (Gradient)

ACE 5 C18, 250 x 4.6mm
ACE-121-2546
A. 50mM KH2PO4 (pH 3.0)
B. MeOH
1.0ml/min
T(mins) %A
%B
0
97
3
5
97
3
45
55
45
50
20
80
Ambient
UV, 205nm
Conditions
Columns:

Column Dimensions:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Compounds
1. Pyridoxamine
4. Nicotinic acid
5. Pyridoxal
6. Impurity
7. Pyridoxine
8. p-Aminobenzoic acid
9. Pantothenic acid (Vitamin B5)
10. Folic acid (Vitamin M)
11. Cyanocobalamin (Vitamin B12)
12. d-Biotin (Vitamin H)
13. Riboflavin (Vitamin B2)
Vitamins - Water Soluble (Rapid Analysis)
Compounds
1. Pyridoxine
3. Pantothenic acid
4. Cyanocobalamin
5. Folic acid
6. d-Biotin
7. Riboflavin
ACE 5 C18, ACE 5 C8,

ACE 5 C4, ACE 5 CN,
ACE 5 Phenyl, ACE 5 AQ,
ACE 5 C18-HL
150 x 4.6mm
A: 20mM KH2PO4 (pH 3.0)
B: MeOH
1.5ml/min
T(mins) %A
%B
0
95
5
20
5
95
Ambient
UV, 205nm
Conditions (a)
Column:
ACE 5 C18, 250 x 4.6mm
Mobile Phase: A: 20mM KH2PO4 (pH 2.9)

B: MeOH
Flow Rate:
1.0ml/min
Gradient:
T(mins) %A
%B

0
97
3

5
97
3

45
55
45

50
20
80
Detection:
UV, 205nm
ACE 5 C18
5 6
6 7
7
2
8
10,11
13
12
ACE 5 C8
3
9
1
2,3
5
4
Compounds
1. Pyridoxine
3. Pantothenic acid
4. Cyanocobalamin
5. Folic acid
6. d-Biotin
7. Riboflavin
Rapid Analysis Conditions (b)

Column:
ACE 3 Phenyl, 20 x 4.6mm
Mobile Phase: A: 20mM KH2PO4 (pH 3.0)

B: MeOH
Flow Rate:
1.5ml/min
Gradient:
T(mins) %A
%B

0
81
19

3
32
68
Detection:
UV, 205nm
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
Vitamins - Water Soluble (Phase Selectivity)
ACE 5 CN
2
7
6,7
13
ACE 3 Phenyl, 20 x 4.6mm
ACE 5 C18-HL
10
5
4
2,3
ACE 5 AQ
11
3
14
4,5
12
ACE 5 Phenyl
Compounds
1. Pyridoxamine
4. Niacinamide (Vitamin B3)
5. Nicotinic acid
6. Pyridoxal
7. Pyridoxine
9. Pantothenic acid (Vitamin B5)
10. Folic acid (Vitamin M)
11. Cyanocobalamin (Vitamin B12)
12. Riboflavin (Vitamin B2)
13. d-Biotin (Vitamin H)
14. Thioctic acid
19.5
20
80
ACE 5 C8, 250 x 4.6mm

ACE-122-2546
A. 50mM KH2PO4 (pH 2.5)
B. MeOH
1.0ml/min
T(mins)
0
3
16.5
%A
100 100
55
%B
0
0
45
Ambient
UV, 205nm
ACE 5 C18, 250 x 4.6mm
Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Temperature:
Detection:
5,6
ACE 5 C4
Vitamins Water Soluble (Gradient)
Time - Minutes
54
55
Vitamins Fat Soluble

Conditions
Column:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Water Soluble Artificial Colors
ACE 5 C18, 250 x 4.6mm

ACE-121-2546
MeOH
1.5ml/min
30C
UV, 280nm
Compounds
1. Vitamin K3
2. Vitamin A
3. Vitamin A acetate
4. Vitamin D2
5. Vitamin D3
6. Vitamin E
7. Vitamin E acetate
8. Vitamin K1
Conditions
Column:
ACE 3 C18, 100 x 4.6mm
Part Number:
ACE-111-1046
Mobile Phase:
A. 3mM TBAB1 and 5mM

KH2PO4 in H2O

B: 5mM TBAB in MeOH
Flow Rate:
0.8ml/min
Gradient:
T(mins) %A
%B

0
55
45

20
30
70

30
55
45

40
55
45
Temperature:
Ambient
Detection:
UV/VIS, 420nm, 520nm and 600nm
1
4
1
7
5
Compounds
1. Amaranth
2. Sunset Yellow
3. Allura Red
4. Red 2G
5. Ponceau 4R
6. Carmoisine
7. Erythrosine
8
3
1
2
Whey Proteins from Whole Milk

Conditions
Column:
Part Number:
Mobile Phase:

Flow Rate:
Gradient:

Compounds
1. alpha-Lactalbumin
2. beta-Lactoglobulin B
3. beta-Lactoglobulin A
ACE 3 C4-300, 150 x 2.1mm

ACE-213-1502
A: 0.5% HCO2H in H2O
B: 0.5% HCO2H in MeCN
0.4ml/min
T(mins) 0
16 17 20
%A
65 57 20 20
%B
35 43 80 80
21
65
35
31
65
35
Temperature:
40C
Detection:
ESI-MS (+ve)
Time - Minutes
Reproduced with kind permission of University of Vienna, Austria
56
ACE
Part Numbers
100 Ultra-Inert Base Deactivated Analytical HPLC Columns

Dimensions
Particle
(mm)
Size (m)
ACE C18
ACE C8
ACE C4
ACE CN
ACE Phenyl
4.6 x 250
5
ACE-121-2546
ACE-122-2546
ACE-123-2546
ACE-124-2546
ACE-125-2546
4.6 x 150
5
ACE-121-1546
ACE-122-1546
ACE-123-1546
ACE-124-1546
ACE-125-1546
4.6 x 150
3
ACE-111-1546
ACE-112-1546
ACE-113-1546
ACE-114-1546
ACE-115-1546
4.6 x 100
5
ACE-121-1046
ACE-122-1046
ACE-123-1046
ACE-124-1046
ACE-125-1046
4.6 x 100
3
ACE-111-1046
ACE-112-1046
ACE-113-1046
ACE-114-1046
ACE-115-1046
4.6 x 75
5
ACE-121-7546
ACE-122-7546
ACE-123-7546
ACE-124-7546
ACE-125-7546
4.6 x 75
3
ACE-111-7546
ACE-112-7546
ACE-113-7546
ACE 114-7546
ACE-115-7546
4.6 x 50
5
ACE-121-0546
ACE-122-0546
ACE-123-0546
ACE-124-0546
ACE-125-0546
4.6 x 50
3
ACE-111-0546
ACE-112-0546
ACE-113-0546
ACE-114-0546
ACE-115-0546
4.6 x 30
5
ACE-121-0346
ACE-122-0346
ACE-123-0346
ACE-124-0346
ACE-125-0346
4.6 x 30
3
ACE-111-0346
ACE-112-0346
ACE-113-0346
ACE-114-0346
ACE-115-0346
4.6 x 20
5
ACE-121-0246
ACE-122-0246
ACE-123-0246
ACE-124-0246
ACE-125-0246
4.6 x 20
3
ACE-111-0246
ACE-112-0246
ACE-113-0246
ACE-114-0246
ACE-115-0246
3.0 x 250
5
ACE-121-2503
ACE-122-2503
ACE-123-2503
ACE-124-2503
ACE-125-2503
3.0 x 150
5
ACE-121-1503
ACE-122-1503
ACE-123-1503
ACE-124-1503
ACE-125-1503
3.0 x 150
3
ACE-111-1503
ACE-112-1503
ACE-113-1503
ACE-114-1503
ACE-115-1503
3.0 x 100
5
ACE-121-1003
ACE-122-1003
ACE-123-1003
ACE-124-1003
ACE-125-1003
3.0 x 100
3
ACE-111-1003
ACE-112-1003
ACE-113-1003
ACE-114-1003
ACE-115-1003
3.0 x 75
5
ACE-121-7503
ACE-122-7503
ACE-123-7503
ACE-124-7503
ACE-125-7503
3.0 x 75
3
ACE-111-7503
ACE-112-7503
ACE-113-7503
ACE-114-7503
ACE-115-7503
3.0 x 50
5
ACE-121-0503
ACE-122-0503
ACE-123-0503
ACE-124-0503
ACE-125-0503
3.0 x 50
3
ACE-111-0503
ACE-112-0503
ACE-113-0503
ACE-114-0503
ACE-115-0503
3.0 x 30
5
ACE-121-0303
ACE-122-0303
ACE-123-0303
ACE-124-0303
ACE-125-0303
3.0 x 30
3
ACE-111-0303
ACE-112-0303
ACE-113-0303
ACE-114-0303
ACE-115-0303
3.0 x 20
5
ACE-121-0203
ACE-122-0203
ACE-123-0203
ACE-124-0203
ACE-125-0203
3.0 x 20
3
ACE-111-0203
ACE-112-0203
ACE-113-0203
ACE-114-0203
ACE-115-0203
2.1 x 250
5
ACE-121-2502
ACE-122-2502
ACE-123-2502
ACE-124-2502
ACE-125-2502
2.1 x 150
5
ACE-121-1502
ACE-122-1502
ACE-123-1502
ACE-124-1502
ACE-125-1502
2.1 x 150
3
ACE-111-1502
ACE-112-1502
ACE-113-1502
ACE-114-1502
ACE-115-1502
2.1 x 100
5
ACE-121-1002
ACE-122-1002
ACE-123-1002
ACE-124-1002
ACE-125-1002
2.1 x 100
3
ACE-111-1002
ACE-112-1002
ACE-113-1002
ACE-114-1002
ACE-115-1002
2.1 x 75
5
ACE-121-7502
ACE-122-7502
ACE-123-7502
ACE-124-7502
ACE-125-7502
2.1 x 75
3
ACE-111-7502
ACE-112-7502
ACE-113-7502
ACE-114-7502
ACE-115-7502
2.1 x 50
5
ACE-121-0502
ACE-122-0502
ACE-123-0502
ACE-124-0502
ACE-125-0502
2.1 x 50
3
ACE-111-0502
ACE-112-0502
ACE-113-0502
ACE-114-0502
ACE-115-0502
2.1 x 30
5
ACE-121-0302
ACE-122-0302
ACE-123-0302
ACE-124-0302
ACE-125-0302
2.1 x 30
3
ACE-111-0302
ACE-112-0302
ACE-113-0302
ACE-114-0302
ACE-115-0302
2.1 x 20
5
ACE-121-0202
ACE-122-0202
ACE-123-0202
ACE-124-0202
ACE-125-0202
2.1 x 20
3
ACE-111-0202
ACE-112-0202
ACE-113-0202
ACE-114-0202
ACE-115-0202
1.0 x 250
5
ACE-121-2501
ACE-122-2501
ACE-123-2501
ACE-124-2501
ACE-125-2501
1.0 x 150
5
ACE-121-1501
ACE-122-1501
ACE-123-1501
ACE-124-1501
ACE-125-1501
1.0 x 150
3
ACE-111-1501
ACE-112-1501
ACE-113-1501
ACE-114-1501
ACE-115-1501
1.0 x 100
5
ACE-121-1001
ACE-122-1001
ACE-123-1001
ACE-124-1001
ACE-125-1001
1.0 x 100
3
ACE-111-1001
ACE-112-1001
ACE-113-1001
ACE-114-1001
ACE-115-1001
1.0 x 75
5
ACE-121-7501
ACE-122-7501
ACE-123-7501
ACE-124-7501
ACE-125-7501
1.0 x 75
3
ACE-111-7501
ACE-112-7501
ACE-113-7501
ACE-114-7501
ACE-115-7501
1.0 x 50
5
ACE-121-0501
ACE-122-0501
ACE-123-0501
ACE-124-0501
ACE-125-0501
1.0 x 50
3
ACE-111-0501
ACE-112-0501
ACE-113-0501
ACE-114-0501
ACE-115-0501

ACE guard column cartridges for analytical columns 3.0 and 4.6 mm ID. Five guard cartridges per pack. Holder (H0005) required.
Dimensions
Particle
(mm)
Size (m)
ACE C18
ACE C8
ACE C4
ACE CN
ACE Phenyl
3.0 x
10
3
ACE-111-0103GD
ACE-112-0103GD
ACE-113-0103GD
ACE-114-0103GD
ACE-115-0103GD
3.0 x
10
5
ACE-121-0103GD
ACE-122-0103GD
ACE-123-0103GD
ACE-124-0103GD
ACE-125-0103GD
Integral guard holder for above H0005
H0005
H0005
H0005
H0005

ACE guard column cartridges for narrow bore columns 2.1 mm ID. Five guard cartridges per pack. Holder (H0004) required.
Dimensions
Particle
(mm)
Size (m)
ACE C18
ACE C8
ACE C4
ACE CN
ACE Phenyl
2.1 x
10
3
ACE-111-0102GD
ACE-112-0102GD
ACE-113-0102GD
ACE-114-0102GD
ACE-115-0102GD
2.1 x
10
5
ACE-121-0102GD
ACE-122-0102GD
ACE-123-0102GD
ACE-124-0102GD
ACE-125-0102GD
Integral guard holder for above H0004
H0004
H0004
H0004
H0004

Dimensions
Particle
(mm)
Size (m)
ACE C18
ACE C8
ACE C4
ACE CN
ACE Phenyl
1.0 x
10
3
ACE-111-0101GD
ACE-112-0101GD
ACE-113-0101GD
ACE-114-0101GD
ACE-115-0101GD
1.0 x
10
5
ACE-121-0101GD
ACE-122-0101GD
ACE-123-0101GD
ACE-124-0101GD
ACE-125-0101GD
Stand-alone guard holder for above H0001
H0001
H0001
H0001
H0001
ACE AQ
ACE-126-2546
ACE-126-1546
ACE-116-1546
ACE-126-1046
ACE-116-1046
ACE-126-7546
ACE-116-7546
ACE-126-0546
ACE-116-0546
ACE-126-0346
ACE-116-0346
ACE-126-0246
ACE-116-0246
ACE-126-2503
ACE-126-1503
ACE-116-1503
ACE-126-1003
ACE-116-1003
ACE-126-7503
ACE-116-7503
ACE-126-0503
ACE-116-0503
ACE-126-0303
ACE-116-0303
ACE-126-0203
ACE-116-0203
ACE-126-2502
ACE-126-1502
ACE-116-1502
ACE-126-1002
ACE-116-1002
ACE-126-7502
ACE-116-7502
ACE-126-0502
ACE-116-0502
ACE-126-0302
ACE-116-0302
ACE-126-0202
ACE-116-0202
ACE-126-2501
ACE-126-1501
ACE-116-1501
ACE-126-1001
ACE-116-1001
ACE-126-7501
ACE-116-7501
ACE-126-0501
ACE-116-0501
ACE AQ
ACE-116-0103GD
ACE-126-0103GD
H0005
ACE AQ
ACE-116-0102GD
ACE-126-0102GD
H0004
ACE AQ
ACE-116-0101GD
ACE-126-0101GD
H0001
Note: A stand-alone guard cartridge holder is available for all the guard cartridges listed above. The part number for this guard cartridge holder is H0001. Connecting tubing and fittings, or a column
coupler (part number C0001), is required to use this stand-alone guard cartridge holder.
ACE Part Numbers
57
ACE
Part Numbers
ACE
Part Numbers
300 Ultra-Inert Base Deactivated Analytical HPLC Columns for Peptides/Proteins
100 Ultra-Inert Base Deactivated Capillary and Nano HPLC Columns
Dimensions
(mm)
4.6 x 250
4.6 x 150
4.6 x 150
4.6 x 100
4.6 x 100
4.6 x 75
4.6 x 75
4.6 x 50
4.6 x 50
4.6 x 30
4.6 x 30
4.6 x 20
4.6 x 20
3.0 x 250
3.0 x 150
3.0 x 150
3.0 x 100
3.0 x 100
3.0 x 75
3.0 x 75
3.0 x 50
3.0 x 50
3.0 x 30
3.0 x 30
3.0 x 20
3.0 x 20
2.1 x 250
2.1 x 150
2.1 x 150
2.1 x 100
2.1 x 100
2.1 x 75
2.1 x 75
2.1 x 50
2.1 x 50
2.1 x 30
2.1 x 30
2.1 x 20
2.1 x 20
1.0 x 250
1.0 x 150
1.0 x 150
1.0 x 100
1.0 x 100
1.0 x 75
1.0 x 75
1.0 x 50
1.0 x 50
Dimensions

(mm)
0.075 x 250
0.075 x 150
0.075 x 150
0.075 x 100
0.075 x 100
0.10 x 250
0.10 x 150
0.10 x 150
0.10 x 100
0.10 x 100
0.30 x 250
0.30 x 150
0.30 x 150
0.30 x 100
0.30 x 100
0.30 x 50
0.30 x 50
0.30 x 30
0.30 x 30
0.50 x 250
0.50 x 150
0.50 x 150
0.50 x 100
0.50 x 100
0.50 x 50
0.50 x 50
0.50 x 30
0.50 x 30
Particle
Size (m)
5
5
3
5
3
5
3
5
3
5
3
5
3
5
5
3
5
3
5
3
5
3
5
3
5
3
5
5
3
5
3
5
3
5
3
5
3
5
3
5
5
3
5
3
5
3
5
3
ACE C18-300
ACE-221-2546
ACE-221-1546
ACE-211-1546
ACE-221-1046
ACE-211-1046
ACE-221-7546
ACE-211-7546
ACE-221-0546
ACE-211-0546
ACE-221-0346
ACE-211-0346
ACE-221-0246
ACE-211-0246
ACE-221-2503
ACE-221-1503
ACE-211-1503
ACE-221-1003
ACE-211-1003
ACE-221-7503
ACE-211-7503
ACE-221-0503
ACE-211-0503
ACE-221-0303
ACE-211-0303
ACE-221-0203
ACE-211-0203
ACE-221-2502
ACE-221-1502
ACE-211-1502
ACE-221-1002
ACE-211-1002
ACE-221-7502
ACE-211-7502
ACE-221-0502
ACE-211-0502
ACE-221-0302
ACE-211-0302
ACE-221-0202
ACE-211-0202
ACE-221-2501
ACE-221-1501
ACE-211-1501
ACE-221-1001
ACE-211-1001
ACE-221-7501
ACE-211-7501
ACE-221-0501
ACE-211-0501
ACE C8-300
ACE-222-2546
ACE-222-1546
ACE-212-1546
ACE-222-1046
ACE-212-1046
ACE-222-7546
ACE-212-7546
ACE-222-0546
ACE-212-0546
ACE-222-0346
ACE-212-0346
ACE-222-0246
ACE-212-0246
ACE-222-2503
ACE-222-1503
ACE-212-1503
ACE-222-1003
ACE-212-1003
ACE-222-7503
ACE-212-7503
ACE-222-0503
ACE-212-0503
ACE-222-0303
ACE-212-0303
ACE-222-0203
ACE-212-0203
ACE-222-2502
ACE-222-1502
ACE-212-1502
ACE-222-1002
ACE-212-1002
ACE-222-7502
ACE-212-7502
ACE-222-0502
ACE-212-0502
ACE-222-0302
ACE-212-0302
ACE-222-0202
ACE-212-0202
ACE-222-2501
ACE-222-1501
ACE-212-1501
ACE-222-1001
ACE-212-1001
ACE-222-7501
ACE-212-7501
ACE-222-0501
ACE-212-0501
ACE C4-300
ACE-223-2546
ACE-223-1546
ACE-213-1546
ACE-223-1046
ACE-213-1046
ACE-223-7546
ACE-213-7546
ACE-223-0546
ACE-213-0546
ACE-223-0346
ACE-213-0346
ACE-223-0246
ACE-213-0246
ACE-223-2503
ACE-223-1503
ACE-213-1503
ACE-223-1003
ACE-213-1003
ACE-223-7503
ACE-213-7503
ACE-223-0503
ACE-213-0503
ACE-223-0303
ACE-213-0303
ACE-223-0203
ACE-213-0203
ACE-223-2502
ACE-223-1502
ACE-213-1502
ACE-223-1002
ACE-213-1002
ACE-223-7502
ACE-213-7502
ACE-223-0502
ACE-213-0502
ACE-223-0302
ACE-213-0302
ACE-223-0202
ACE-213-0202
ACE-223-2501
ACE-223-1501
ACE-213-1501
ACE-223-1001
ACE-213-1001
ACE-223-7501
ACE-213-7501
ACE-223-0501
ACE-213-0501
ACE CN-300
ACE-224-2546
ACE-224-1546
ACE-214-1546
ACE-224-1046
ACE-214-1046
ACE-224-7546
ACE 214-7546
ACE-224-0546
ACE-214-0546
ACE-224-0346
ACE-214-0346
ACE-224-0246
ACE-214-0246
ACE-224-2503
ACE-224-1503
ACE-214-1503
ACE-224-1003
ACE-214-1003
ACE-224-7503
ACE-214-7503
ACE-224-0503
ACE-214-0503
ACE-224-0303
ACE-214-0303
ACE-224-0203
ACE-214-0203
ACE-224-2502
ACE-224-1502
ACE-214-1502
ACE-224-1002
ACE-214-1002
ACE-224-7502
ACE-214-7502
ACE-224-0502
ACE-214-0502
ACE-224-0302
ACE-214-0302
ACE-224-0202
ACE-214-0202
ACE-224-2501
ACE-224-1501
ACE-214-1501
ACE-224-1001
ACE-214-1001
ACE-224-7501
ACE-214-7501
ACE-224-0501
ACE-214-0501
ACE Phenyl-300
ACE-225-2546
ACE-225-1546
ACE-215-1546
ACE-225-1046
ACE-215-1046
ACE-225-7546
ACE-215-7546
ACE-225-0546
ACE-215-0546
ACE-225-0346
ACE-215-0346
ACE-225-0246
ACE-215-0246
ACE-225-2503
ACE-225-1503
ACE-215-1503
ACE-225-1003
ACE-215-1003
ACE-225-7503
ACE-215-7503
ACE-225-0503
ACE-215-0503
ACE-225-0303
ACE-215-0303
ACE-225-0203
ACE-215-0203
ACE-225-2502
ACE-225-1502
ACE-215-1502
ACE-225-1002
ACE-215-1002
ACE-225-7502
ACE-215-7502
ACE-225-0502
ACE-215-0502
ACE-225-0302
ACE-215-0302
ACE-225-0202
ACE-215-0202
ACE-225-2501
ACE-225-1501
ACE-215-1501
ACE-225-1001
ACE-215-1001
ACE-225-7501
ACE-215-7501
ACE-225-0501
ACE-215-0501
ACE guard column cartridges for analytical columns 3.0 and 4.6 mm ID. Five guard cartridges per pack. Holder (H0005) required.
Dimensions
Particle
(mm)
Size (m)
ACE C18-300
ACE C8-300
ACE C4-300
ACE CN-300
3.0 x
10
3
ACE-211-0103GD
ACE-212-0103GD
ACE-213-0103GD
ACE-214-0103GD
3.0 x
10
5
ACE-221-0103GD
ACE-222-0103GD
ACE-223-0103GD
ACE-224-0103GD
Integral g
uard holder for above
H0005
H0005
H0005
H0005
ACE Phenyl-300
ACE-215-0103GD
ACE-225-0103GD
H0005
Dimensions
Particle
(mm)
Size (m)
ACE C18-300
ACE C8-300
ACE C4-300
ACE CN-300
2.1 x
10
3
ACE-211-0102GD
ACE-212-0102GD
ACE-213-0102GD
ACE-214-0102GD
2.1 x
10
5
ACE-221-0102GD
ACE-222-0102GD
ACE-223-0102GD
ACE-224-0102GD
Integral g
uard holder for above
H0004
H0004
H0004
H0004
ACE Phenyl-300
ACE-215-0102GD
ACE-225-0102GD
H0004
Dimensions
Particle
(mm)
Size (m)
ACE C18-300
ACE C8-300
ACE C4-300
ACE CN-300
1.0 x
10
3
ACE-211-0101GD
ACE-212-0101GD
ACE-213-0101GD
ACE-214-0101GD
1.0 x
10
5
ACE-221-0101GD
ACE-222-0101GD
ACE-223-0101GD
ACE-224-0101GD
Stand-alone guard holder for above H0001
H0001
H0001
H0001
ACE Phenyl-300
ACE-215-0101GD
ACE-225-0101GD
H0001
Particle
Size (m)
5
5
3
5
3
5
5
3
5
3
5
5
3
5
3
5
3
5
3
5
5
3
5
3
5
3
5
3
ACE C18
ACE-121-2500075
ACE-121-1500075
ACE-111-1500075
ACE-121-1000075
ACE-111-1000075
ACE-121-25001
ACE-121-15001
ACE-111-15001
ACE-121-10001
ACE-111-10001
ACE-121-25003
ACE-121-15003
ACE-111-15003
ACE-121-10003
ACE-111-10003
ACE-121-05003
ACE-111-05003
ACE-121-03003
ACE-111-03003
ACE-121-25005
ACE-121-15005
ACE-111-15005
ACE-121-10005
ACE-111-10005
ACE-121-05005
ACE-111-05005
ACE-121-03005
ACE-111-03005
ACE C8
ACE-122-2500075
ACE-122-1500075
ACE-112-1500075
ACE-122-1000075
ACE-112-1000075
ACE-122-25001
ACE-122-15001
ACE-112-15001
ACE-122-10001
ACE-112-10001
ACE-122-25003
ACE-122-15003
ACE-112-15003
ACE-122-10003
ACE-112-10003
ACE-122-05003
ACE-112-05003
ACE-122-03003
ACE-112-03003
ACE-122-25005
ACE-122-15005
ACE-112-15005
ACE-122-10005
ACE-112-10005
ACE-122-05005
ACE-112-05005
ACE-122-03005
ACE-112-03005
ACE C4
ACE-123-2500075
ACE-123-1500075
ACE-113-1500075
ACE-123-1000075
ACE-113-1000075
ACE-123-25001
ACE-123-15001
ACE-113-15001
ACE-123-10001
ACE-113-10001
ACE-123-25003
ACE-123-15003
ACE-113-15003
ACE-123-10003
ACE-113-10003
ACE-123-05003
ACE-113-05003
ACE-123-03003
ACE-113-03003
ACE-123-25005
ACE-123-15005
ACE-113-15005
ACE-123-10005
ACE-113-10005
ACE-123-05005
ACE-113-05005
ACE-123-03005
ACE-113-03005
ACE CN
ACE-124-2500075
ACE-124-1500075
ACE-114-1500075
ACE-124-1000075
ACE-114-1000075
ACE-124-25001
ACE-124-15001
ACE-114-15001
ACE-124-10001
ACE-114-10001
ACE-124-25003
ACE-124-15003
ACE-114-15003
ACE-124-10003
ACE-114-10003
ACE-124-05003
ACE-114-05003
ACE-124-03003
ACE-114-03003
ACE-124-25005
ACE-124-15005
ACE-114-15005
ACE-124-10005
ACE-114-10005
ACE-124-05005
ACE-114-05005
ACE-124-03005
ACE-114-03005
ACE Phenyl
ACE-125-2500075
ACE-125-1500075
ACE-115-1500075
ACE-125-1000075
ACE-115-1000075
ACE-125-25001
ACE-125-15001
ACE-115-15001
ACE-125-10001
ACE-115-10001
ACE-125-25003
ACE-125-15003
ACE-115-15003
ACE-125-10003
ACE-115-10003
ACE-125-05003
ACE-115-05003
ACE-125-03003
ACE-115-03003
ACE-125-25005
ACE-125-15005
ACE-115-15005
ACE-125-10005
ACE-115-10005
ACE-125-05005
ACE-115-05005
ACE-125-03005
ACE-115-03005
ACE AQ
ACE-126-2500075
ACE-126-1500075
ACE-116-1500075
ACE-126-1000075
ACE-116-1000075
ACE-126-25001
ACE-126-15001
ACE-116-15001
ACE-126-10001
ACE-116-10001
ACE-126-25003
ACE-126-15003
ACE-116-15003
ACE-126-10003
ACE-116-10003
ACE-126-05003
ACE-116-05003
ACE-126-03003
ACE-116-03003
ACE-126-25005
ACE-126-15005
ACE-116-15005
ACE-126-10005
ACE-116-10005
ACE-126-05005
ACE-116-05005
ACE-126-03005
ACE-116-03005
300 Ultra-Inert Base Deactivated Capillary and Nano HPLC Columns

for Peptides/Proteins
Dimensions

(mm)
0.075 x 250
0.075 x 150
0.075 x 150
0.075 x 100
0.075 x 100
0.10 x 250
0.10 x 150
0.10 x 150
0.10 x 100
0.10 x 100
0.30 x 250
0.30 x 150
0.30 x 150
0.30 x 100
0.30 x 100
0.30 x 50
0.30 x 50
0.30 x 30
0.30 x 30
0.50 x 250
0.50 x 150
0.50 x 150
0.50 x 100
0.50 x 100
0.50 x 50
0.50 x 50
0.50 x 30
0.50 x 30
Particle
Size (m)
5
5
3
5
3
5
5
3
5
3
5
5
3
5
3
5
3
5
3
5
5
3
5
3
5
3
5
3
ACE C18-300
ACE-221-2500075
ACE-221-1500075
ACE-211-1500075
ACE-221-1000075
ACE-211-1000075
ACE-221-25001
ACE-221-15001
ACE-211-15001
ACE-221-10001
ACE-211-10001
ACE-221-25003
ACE-221-15003
ACE-211-15003
ACE-221-10003
ACE-211-10003
ACE-221-05003
ACE-211-05003
ACE-221-03003
ACE-211-03003
ACE-221-25005
ACE-221-15005
ACE-211-15005
ACE-221-10005
ACE-211-10005
ACE-221-05005
ACE-211-05005
ACE-221-03005
ACE-211-03005
ACE C8-300
ACE-222-2500075
ACE-222-1500075
ACE-212-1500075
ACE-222-1000075
ACE-212-1000075
ACE-222-25001
ACE-222-15001
ACE-212-15001
ACE-222-10001
ACE-212-10001
ACE-222-25003
ACE-222-15003
ACE-212-15003
ACE-222-10003
ACE-212-10003
ACE-222-05003
ACE-212-05003
ACE-222-03003
ACE-212-03003
ACE-222-25005
ACE-222-15005
ACE-212-15005
ACE-222-10005
ACE-212-10005
ACE-222-05005
ACE-212-05005
ACE-222-03005
ACE-212-03005
ACE C4-300
ACE-223-2500075
ACE-223-1500075
ACE-213-1500075
ACE-223-1000075
ACE-213-1000075
ACE-223-25001
ACE-223-15001
ACE-213-15001
ACE-223-10001
ACE-213-10001
ACE-223-25003
ACE-223-15003
ACE-213-15003
ACE-223-10003
ACE-213-10003
ACE-223-05003
ACE-213-05003
ACE-223-03003
ACE-213-03003
ACE-223-25005
ACE-223-15005
ACE-213-15005
ACE-223-10005
ACE-213-10005
ACE-223-05005
ACE-213-05005
ACE-223-03005
ACE-213-03005
ACE CN-300
ACE-224-2500075
ACE-224-1500075
ACE-214-1500075
ACE-224-1000075
ACE-214-1000075
ACE-224-25001
ACE-224-15001
ACE-214-15001
ACE-224-10001
ACE-214-10001
ACE-224-25003
ACE-224-15003
ACE-214-15003
ACE-224-10003
ACE-214-10003
ACE-224-05003
ACE-214-05003
ACE-224-03003
ACE-214-03003
ACE-224-25005
ACE-224-15005
ACE-214-15005
ACE-224-10005
ACE-214-10005
ACE-224-05005
ACE-214-05005
ACE-224-03005
ACE-214-03005
ACE Phenyl-300
ACE-225-2500075
ACE-225-1500075
ACE-215-1500075
ACE-225-1000075
ACE-215-1000075
ACE-225-25001
ACE-225-15001
ACE-215-15001
ACE-225-10001
ACE-215-10001
ACE-225-25003
ACE-225-15003
ACE-215-15003
ACE-225-10003
ACE-215-10003
ACE-225-05003
ACE-215-05003
ACE-225-03003
ACE-215-03003
ACE-225-25005
ACE-225-15005
ACE-215-15005
ACE-225-10005
ACE-215-10005
ACE-225-05005
ACE-215-05005
ACE-225-03005
ACE-215-03005
Note: A stand-alone guard cartridge holder is available for all the guard cartridges listed above. The part number for this guard cartridge holder is H0001. Connecting tubing and fittings, or a column
coupler (part number C0001), is required to use this stand-alone guard cartridge holder.
58
ACE Part Numbers
ACE Part Numbers
59
ACE
Notes
Part Numbers
100 Preparative Ultra-Inert Base Deactivated HPLC Columns

Dimensions
(mm)
10.0 x 50
10.0 x 75
10.0 x 100
10.0 x 150
10.0 x 250
21.2 x 50
21.2 x 50
21.2 x 75
21.2 x 75
21.2 x 100
21.2 x 100
21.2 x 150
21.2 x 150
21.2 x 250
21.2 x 250
30.0 x 50
30.0 x 100
30.0 x 150
30.0 x 250
Particle
Size (m)
5
5
5
5
5
5
10
5
10
5
10
5
10
5
10
10
10
10
10
ACE C18-HL
ACE-321-0510
ACE-321-7510
ACE-321-1010
ACE-321-1510
ACE-321-2510
ACE-321-0520
ACE-331-0520
ACE-321-7520
ACE-331-7520
ACE-321-1020
ACE-331-1020
ACE-321-1520
ACE-331-1520
ACE-321-2520
ACE-331-2520
ACE-331-0530
ACE-331-1030
ACE-331-1530
ACE-331-2530
ACE C18
ACE-121-0510
ACE-121-7510
ACE-121-1010
ACE-121-1510
ACE-121-2510
ACE-121-0520
ACE-131-0520
ACE-121-7520
ACE-131-7520
ACE-121-1020
ACE-131-1020
ACE-121-1520
ACE-131-1520
ACE-121-2520
ACE-131-2520
ACE-131-0530
ACE-131-1030
ACE-131-1530
ACE-131-2530
ACE C8
ACE-122-0510
ACE-122-7510
ACE-122-1010
ACE-122-1510
ACE-122-2510
ACE-122-0520
ACE-132-0520
ACE-122-7520
ACE-132-7520
ACE-122-1020
ACE-132-1020
ACE-122-1520
ACE-132-1520
ACE-122-2520
ACE-132-2520
ACE-132-0530
ACE-132-1030
ACE-132-1530
ACE-132-2530
ACE C4
ACE-123-0510
ACE-123-7510
ACE-123-1010
ACE-123-1510
ACE-123-2510
ACE-123-0520
ACE-133-0520
ACE-123-7520
ACE-133-7520
ACE-123-1020
ACE-133-1020
ACE-123-1520
ACE-133-1520
ACE-123-2520
ACE-133-2520
ACE-133-0530
ACE-133-1030
ACE-133-1530
ACE-133-2530
ACE CN
ACE-124-0510
ACE-124-7510
ACE-124-1010
ACE-124-1510
ACE-124-2510
ACE-124-0520
ACE-134-0520
ACE-124-7520
ACE-134-7520
ACE-124-1020
ACE-134-1020
ACE-124-1520
ACE-134-1520
ACE-124-2520
ACE-134-2520
ACE-134-0530
ACE-134-1030
ACE-134-1530
ACE-134-2530
ACE Phenyl
ACE-125-0510
ACE-125-7510
ACE-125-1010
ACE-125-1510
ACE-125-2510
ACE-125-0520
ACE-135-0520
ACE-125-7520
ACE-135-7520
ACE-125-1020
ACE-135-1020
ACE-125-1520
ACE-135-1520
ACE-125-2520
ACE-135-2520
ACE-135-0530
ACE-135-1030
ACE-135-1530
ACE-135-2530
ACE AQ
ACE-126-0510
ACE-126-7510
ACE-126-1010
ACE-126-1510
ACE-126-2510
ACE-126-0520
ACE-136-0520
ACE-126-7520
ACE-136-7520
ACE-126-1020
ACE-136-1020
ACE-126-1520
ACE-136-1520
ACE-126-2520
ACE-136-2520
ACE-136-0530
ACE-136-1030
ACE-136-1530
ACE-136-2530
ACE guard column cartridges for preparative columns 10.0 or 21.2 mm ID. Three guard cartridges per pack. Holder (H0002) required.
Dimensions
Particle
(mm)
Size (m)
ACE C18-HL
ACE C18
ACE C8
ACE C4
ACE CN
ACE Phenyl
10 x
10
5
ACE-321-0110GD ACE-121-0110GD
ACE-122-0110GD ACE-123-0110GD ACE-124-0110GD ACE-125-0110GD
10 x
10
10
ACE-331-0110GD ACE-131-0110GD
ACE-132-0110GD ACE-133-0110GD ACE-134-0110GD ACE-135-0110GD
Guard holder for above
H0002
H0002
H0002
H0002
H0002
H0002
ACE AQ
ACE-126-0110GD
ACE-136-0110GD
H0002
ACE guard column cartridges for preparative columns 30.0 mm ID. A single guard cartridge. Holder (H0006) required.
Dimensions
Particle
(mm)
Size (m)
ACE C18-HL
ACE C18
ACE C8
ACE C4
ACE CN
20 x 20
5
ACE-321-0220GD ACE-121-0220GD ACE-122-0220GD ACE-123-0220GD ACE-124-0220GD
20 x 20
10
ACE-331-0220GD ACE-131-0220GD ACE-132-0220GD ACE-133-0220GD ACE-134-0220GD
H0006
H0006
H0006
H0006
H0006
ACE AQ
ACE-126-0220GD
ACE-136-0220GD
H0006
ACE Phenyl
ACE-125-0220GD
ACE-135-0220GD
H0006
300 Preparative Ultra-Inert Base Deactivated HPLC Columns for Peptides/Proteins

Dimensions
(mm)
10.0 x 50
10.0 x 75
10.0 x 100
10.0 x 150
10.0 x 250
21.2 x 50
21.2 x 50
21.2 x 75
21.2 x 75
21.2 x 100
21.2 x 100
21.2 x 150
21.2 x 150
21.2 x 250
21.2 x 250
Particle
Size (m)
5
5
5
5
5
5
10
5
10
5
10
5
10
5
10
ACE C18-300
ACE-221-0510
ACE-221-7510
ACE-221-1010
ACE-221-1510
ACE-221-2510
ACE-221-0520
ACE-231-0520
ACE-221-7520
ACE-231-7520
ACE-221-1020
ACE-231-1020
ACE-221-1520
ACE-231-1520
ACE-221-2520
ACE-231-2520
ACE C8-300
ACE-222-0510
ACE-222-7510
ACE-222-1010
ACE-222-1510
ACE-222-2510
ACE-222-0520
ACE-232-0520
ACE-222-7520
ACE-232-7520
ACE-222-1020
ACE-232-1020
ACE-222-1520
ACE-232-1520
ACE-222-2520
ACE-232-2520
ACE C4-300
ACE-223-0510
ACE-223-7510
ACE-223-1010
ACE-223-1510
ACE-223-2510
ACE-223-0520
ACE-233-0520
ACE-223-7520
ACE-233-7520
ACE-223-1020
ACE-233-1020
ACE-223-1520
ACE-233-1520
ACE-223-2520
ACE-233-2520
ACE CN-300
ACE-224-0510
ACE-224-7510
ACE-224-1010
ACE-224-1510
ACE-224-2510
ACE-224-0520
ACE-234-0520
ACE-224-7520
ACE-234-7520
ACE-224-1020
ACE-234-1020
ACE-224-1520
ACE-234-1520
ACE-224-2520
ACE-234-2520
CE guard column cartridges for preparative columns 10.0 or 21.2 mm ID. Three guard cartridges per pack. Holder (H0002) required.
A
Dimensions
Particle
(mm)
Size (m)
ACE C18-300
ACE C8-300
ACE C4-300
ACE CN-300
10 x
10
5
ACE-221-0110GD
ACE-222-0110GD
ACE-223-0110GD
ACE-224-0110GD
10 x
10
10
ACE-231-0110GD
ACE-232-0110GD
ACE-233-0110GD
ACE-234-0110GD
H0002
H0002
H0002
H0002
Free HPLC Technical Guides and Slide Chart
ACE Phenyl-300
ACE-225-0510
ACE-225-7510
ACE-225-1010
ACE-225-1510
ACE-225-2510
ACE-225-0520
ACE-235-0520
ACE-225-7520
ACE-235-7520
ACE-225-1020
ACE-235-1020
ACE-225-1520
ACE-235-1520
ACE-225-2520
ACE-235-2520
ACE Phenyl-300
ACE-225-0110GD
ACE-235-0110GD
H0002
To receive your free copies of these guides or the HPLC Columns slide chart,
call MAC-MOD Analytical at 1.800.441.7508 or visit www.mac-mod.com.
ACE is a registered trademark of Advanced Chromatography Technologies (ACT).
60
ACE Part Numbers
analytical inc.
MAC-MOD Analytical
103 Commons Court
P.O. Box 587
Chadds Ford, PA 19317
Phone: 1.800.441.7508
Fax: 1.610.358.5993
E-mail: info@mac-mod.com
Web Site: www.mac-mod.com
JANUARY 2008 LC492
HPLC Columns

ACE® Ultra-Inert Base Deactivated HPLC Columns

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ACE

Product Catalog & Application Guide

Ultra-Inert Base Deactivated Columns

ACE Ultra-Inert Base

For Technical Assistance

Introduction and Product Overview

Rapid Analysis and LC/MS Columns

Wide Pore (300 ) Columns for Peptides and Proteins

Capillary and Nano Columns

Preparative HPLC Columns

Integral Guard Columns

Index by Application Type

Ultra-Inert Base Deactivated HPLC Columns

ACE Stationary Phases Virtually Eliminate the Negative

ACE HPLC Columns offer unparalleled performance and guaranteed

ACE C18 4.6 x 250 mm, 5 m

Figure 3 Asymmetry Comparison

ACE Columns provide excellent peak shape and efficiency

Figure 2 Peak Tailing Interaction

HyPURITY Elite C18

4.6 x 150 mm, 5 m

Mobile Phase: 80% Methanol

Eclipse XDB C18

Acidic silanols on the surface of silica stationary phase

ACE Ultra-Inert Base Deactivated HPLC Columns

MAC-MOD recommends starting most method development projects with C8,

Capillary, Rapid Analysis, LC/MS and Preparative Columns

Specifications for ACE Phases

Need Even More Resolution? Choose 3 m ACE Columns

Choose ACE AQ Columns for Polar Retention

All ACE bonded phases are available in 3 m, 5 m, and 10 m particle sizes. So

Mobile Phase: 80% MeOH

Performance excellent peak shape for basic and acidic compounds

Choosing the Bonded Phase Thats Best for Your Application

ACE 4.6 x 250 mm, 5 m

Mobile Phase: 20% 0.025M KH2PO4, pH 6.0

Amitriptyline is commonly used to demonstrate

ACE Ultra-Inert Base Deactivated HPLC Columns

Excellent Chemical Stability:

At low pH, column deterioration is caused by

Figure 5 Acid Stability, pH 1.8

Subtle changes in silanol activity are one of the

Acidic Exposure Conditions:

Mobile Phase: 50% CH3CN

A series of stringent quality assurance tests are

Figure 6 ACE CN Stability at pH 1.8

Figure 7 ACE C18 Stability at pH 11.0

Day 90 - N0.5 (tol) = 112,000 pl/m

ACE C18 4.6 x 150 mm

ACE Ultra-Inert Base Deactivated HPLC Columns

Mobile Phase: 50% CH3CN

Mobile Phase: 50% CH3CN

Excellent Chemical Stability:

At high pH, column degradation is caused by

Fully Validated Columns

Basic Exposure Conditions:

ACE Ultra-Inert Base Deactivated HPLC Columns

ACE Ultra-Inert Base Deactivated HPLC Columns

ACE Ultra-Inert Base Deactivated HPLC Columns

By eliminating silanol interference, mobile phase additives that can

Rapid Analysis and LC/MS Columns

20 mm, 30 mm and 50 mm column lengths

Figure 10 Independent Column Comparison of HPLC Columns

Configured for High Sample Throughput