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ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Section Number: 07.01
Topic: Microbial Nutrition
2. Microorganisms require small quantities of ______ for enzyme function and maintenance
of protein structure:
A. electrolytes
B. macronutrients
C. water
D. growth factors
E. trace elements
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Section Number: 07.01
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.05 Compare and contrast the processes of diffusion and osmosis.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.01 List the essential nutrients of a bacterial cell.
Section Number: 07.01
Topic: Microbial Nutrition
6. An organic nutrient that cannot be synthesized by the organism and must be provided is
called a/n
A. element.
B. macronutrient.
C. compound.
D. growth factor.
E. trace element.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.01 List the essential nutrients of a bacterial cell.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.01 List the essential nutrients of a bacterial cell.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.01 List the essential nutrients of a bacterial cell.
Section Number: 07.01
Topic: Microbial Nutrition
11. An organism that uses CO2 for its carbon needs and sunlight for its energy needs would be
called a
A. heterotroph.
B. photoautotroph.
C. chemoheterotroph.
D. saprobe.
E. halotroph.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 05.01 Microorganisms are ubiquitous and live in diverse and dynamic ecosystems.
ASM Topic: Module 03 Metabolic Pathways
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
13. Organisms that feed on dead organisms for nutrients are called
A. saprobes.
B. parasites.
C. autotrophs.
D. lithoautotrophs.
E. phototrophs.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 06.01 Microbes are essential for life as we know it and the processes that support life (e.g., in biogeochemical cycles and
plant and/or animal microflora).
ASM Topic: Module 03 Metabolic Pathways
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.04 Define saprobe and parasite, and provide microbial examples of each.
Section Number: 07.01
Topic: Microbial Ecology
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 06.01 Microbes are essential for life as we know it and the processes that support life (e.g., in biogeochemical cycles and
plant and/or animal microflora).
ASM Topic: Module 03 Metabolic Pathways
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.01
Topic: Microbial Nutrition
16. Microorganisms that live in severe habitats, such as very hot, acidic, or salty
environments, are called
A. thermophiles.
B. halophiles.
C. psychrophiles.
D. extremophiles.
E. barophiles.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 03 Metabolic Pathways
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.01
Topic: Environmental Influences
Topic: Microbial Nutrition
17. Organisms called _____ live on or in the body of a host, and cause some degree of harm.
A. mesophiles
B. thermophiles
C. commensals
D. pathogens
E. halophiles
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in beneficial, neutral or
detrimental ways.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.04 Define saprobe and parasite, and provide microbial examples of each.
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
20. The movement of molecules from an area of high concentration to an area of lower
concentration is called
A. facilitated diffusion.
B. diffusion.
C. active transport.
D. osmosis.
E. endocytosis.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
22. The movement of substances from lower to higher concentration across a semipermeable
membrane that must have a specific protein carrier and cell expenditure of energy is called
A. facilitated diffusion.
B. diffusion.
C. active transport.
D. osmosis.
E. endocytosis.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
23. The movement of substances from higher to lower concentration across a semipermeable
membrane that must have a specific protein carrier but no energy expenditure is called
A. facilitated diffusion.
B. diffusion.
C. active transport.
D. osmosis.
E. endocytosis.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
24. The process by which a cell uses ATP to enclose a substance in its membrane, by forming
a vacuole and engulfing it, is called:
A. facilitated diffusion.
B. diffusion.
C. active transport.
D. osmosis.
E. endocytosis.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
25. Bacteria living in a freshwater stream that are moved to salty seawater would
A. be in a hypotonic solution.
B. gain water.
C. be in an isotonic solution.
D. become dehydrated due to the loss of water.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 3. Apply
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.01
Topic: Cellular Transport
26. Which of the following microorganisms would find hypotonic conditions most
detrimental?
A. bacteria
B. protozoa
C. fungi
D. algae
E. cyanobacteria
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 3. Apply
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
Blooms Level: 1. Remember
Blooms Level: 6. Create
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 02.04 While microscopic eukaryotes (for example, fungi, protozoa, and algae) carry out some of the same processes as
bacteria, many of the cellular properties are fundamentally different.
ASM Topic: Module 02 Structure and Function
Blooms Level: 1. Remember
Learning Outcome: 07.05 Compare and contrast the processes of diffusion and osmosis.
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 02.03 Bacteria and Archaea have specialized structures (e.g., flagella, endospores, and pili) that often confer critical
capabilities.
ASM Objective: 02.04 While microscopic eukaryotes (for example, fungi, protozoa, and algae) carry out some of the same processes as
bacteria, many of the cellular properties are fundamentally different.
ASM Topic: Module 02 Structure and Function
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
31. When whole cells or large molecules in solution are engulfed by a cell, this endocytosis is
specifically termed
A. pinocytosis.
B. phagocytosis.
C. facilitated transport.
D. facilitated diffusion.
E. exocytosis.
ASM Objective: 03.04 The growth of microorganisms can be controlled by physical, chemical, mechanical, or biological means.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
32. Mediated transport of polar molecules and ions across the plasma membrane utilizes a/n
_____ carrier that will bind to the substance, effecting a conformational change that allows
movement across the membrane.
A. protein
B. lipid
C. porin
D. enzyme
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 02 Structure and Function
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Cellular Transport
33. In _____ conditions, the cell wall will help prevent the cell from bursting.
A. hypertonic
B. hypotonic
C. isotonic
D. mesotonic
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.01
Topic: Cellular Transport
34. Cultures of a bacterial species were incubated on the shelf of a refrigerator (5oC), out on a
lab bench top (22oC), on the shelf of a 37 C incubator, and on the shelf of a 50 C incubator.
After incubation, there was no growth at 37 C and 50 C, slight growth in the refrigerator, and
abundant growth on the lab bench top. What term could be used for this species?
A. halophile
B. mesophile
C. anaerobe
D. psychrotroph
E. capnophile
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
35. Cultures of a bacterial species were incubated in a standard incubator, in an anaerobic jar,
and in a candle jar. After incubation, there was moderate growth of cultures in the candle and
anaerobic jars, but heavy growth of the culture in the incubator. This species is a/n
A. aerobe.
B. anaerobe.
C. facultative anaerobe.
D. microaerophile.
E. capnophile.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
36. A microorganism that has an optimum growth temperature of 37 C, but can survive short
exposure to high temperatures is called a/n
A. extremophile.
B. thermophile.
C. psychrophile.
D. facultative psychrophile.
E. thermoduric microbe.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
37. An organism that grows slowly in the cold but has an optimum growth temperature of 32
C is called a/n
A. extremophile.
B. thermophile.
C. psychrophile.
D. facultative psychrophile.
E. thermoduric.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 2. Understand
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
40. All of the following could find a location in or on body tissues suitable for growth except
A. psychrophiles.
B. anaerobes.
C. facultative anaerobes.
D. mesophiles.
E. capnophiles.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
41. A microorganism that does not have catalase or superoxide dismutase would find it
difficult to live in an environment with
A. carbon dioxide.
B. oxygen.
C. high salt.
D. temperatures above 37 C.
E. high acidity.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 2. Understand
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
42. A microaerophile
A. grows best in an anaerobic jar.
B. grows with or without oxygen.
C. needs normal atmospheric levels of oxygen.
D. requires a small amount of oxygen but won't grow at normal atmospheric levels of oxygen.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
43. The toxic superoxide ion is converted to harmless oxygen by two enzymes,
A. catalase and hydrogen peroxidases.
B. superoxide dismutase and hydrogen peroxidase.
C. superoxide dismutase and catalase.
D. catalase and oxidase.
E. superoxide dismutase and oxidase.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
44. An organism that can use gaseous oxygen in metabolism and has the enzymes to process
toxic oxygen products is a/n
A. aerobe.
B. obligate aerobe.
C. facultative anaerobe.
D. microaerophile.
E. obligate anaerobe.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
45. An organism that can exist in both oxygen and oxygen-free environments is a/n
A. aerobe.
B. obligate aerobe.
C. facultative anaerobe.
D. microaerophile.
E. obligate anaerobe.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Blooms Level: 1. Remember
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Microbial Growth
ASM Objective: 05.01 Microorganisms are ubiquitous and live in diverse and dynamic ecosystems.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
51. E. coli bacteria that normally live in the human large intestines and produce vitamin K
that the body uses would be best termed a _____ relationship.
A. parasitic
B. saprobic
C. antagonistic
D. mutualistic
ASM Objective: 05.01 Microorganisms are ubiquitous and live in diverse and dynamic ecosystems.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 2. Understand
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
53. When microbes live independently but cooperate and share nutrients, it is called
A. symbiosis.
B. satellitism.
C. antibiosis.
D. mutualism.
E. synergism.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
54. When microbes are in a close nutritional relationship, and one benefits but the other is not
harmed, it is called
A. symbiosis.
B. satellitism.
C. commensalism.
D. mutualism.
E. synergism.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
55. A change in one partner, in a close relationship, that leads to a change in the other partner
is
A. symbiosis.
B. coevolution.
C. antibiosis.
D. mutualism.
E. synergism.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
56. The time interval from parent cell to two new daughter cells is called
A. binary fission.
B. growth curve.
C. generation time.
D. death phase.
E. culture time.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.14 Define doubling time, and describe how it leads to exponential growth.
Section Number: 07.03
Topic: Microbial Growth
57. The phase of the bacterial growth curve in which the rate of multiplication equals the rate
of cell death is the
A. lag phase.
B. log phase.
C. stationary phase.
D. death phase.
E. telophase.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.15 Compare and contrast the four phases of growth in a bacterial growth curve.
Section Number: 07.03
Topic: Microbial Growth
58. The phase of the bacterial growth curve in which newly inoculated cells are adjusting to
their new environment, metabolizing but not growing at an exponential rate, is the
A. lag phase.
B. log phase.
C. stationary phase.
D. death phase.
E. prophase.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.15 Compare and contrast the four phases of growth in a bacterial growth curve.
Section Number: 07.03
Topic: Microbial Growth
59. The phase of the bacterial growth curve that shows the maximum rate of cell division is
the
A. lag phase.
B. log phase.
C. stationary phase.
D. death phase.
E. prophase.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.15 Compare and contrast the four phases of growth in a bacterial growth curve.
Section Number: 07.03
Topic: Microbial Growth
60. In the viable plate count method, a measured sample of a culture is evenly spread across
an agar surface and incubated. Each _____ represents one _____ from the sample.
A. cell; cell
B. cell; colony
C. colony; cell
D. species; colony
E. generation; cell
ASM Objective: 08.04 Estimate the number of microorganisms in a sample (using, for example, direct count, viable plate count, and
spectrophotometric methods).
ASM Topic: Module 08 Microbiology Skills
Blooms Level: 1. Remember
Learning Outcome: 07.16 Identify one quantitative and one qualitative method used for analyzing bacterial growth.
Section Number: 07.03
Topic: Microbial Growth
61. When it is important to count the number of cells, determine cell size, and differentiate
between dead and live cells, a/n ___ is used.
A. coulter counter
B. flow cytometer
C. SEM
D. methylene dye indicator
E. turbidity counter
ASM Objective: 08.04 Estimate the number of microorganisms in a sample (using, for example, direct count, viable plate count, and
spectrophotometric methods).
ASM Topic: Module 08 Microbiology Skills
Blooms Level: 2. Understand
Learning Outcome: 07.16 Identify one quantitative and one qualitative method used for analyzing bacterial growth.
Section Number: 07.03
Topic: Microbial Growth
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.02 Differentiate between macronutrients and micronutrients.
Section Number: 07.01
Topic: Microbial Nutrition
63. Most microorganisms on earth can only live and survive in habitats that are similar to
human body conditions.
FALSE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.01 List the essential nutrients of a bacterial cell.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 03 Metabolic Pathways
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.04 Define saprobe and parasite, and provide microbial examples of each.
Section Number: 07.01
Topic: Microbial Nutrition
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.04 Define saprobe and parasite, and provide microbial examples of each.
Section Number: 07.01
Topic: Microbial Nutrition
69. Saprobes do not need a carbon source for growth and metabolism.
FALSE
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.04 Define saprobe and parasite, and provide microbial examples of each.
Section Number: 07.01
Topic: Microbial Nutrition
70. Lithoautotrophs use inorganic nutrients for carbon and energy sources.
TRUE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
71. Facilitated diffusion and active transport require a carrier protein to mediate the
movement across the plasma membrane.
TRUE
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 1. Remember
Learning Outcome: 07.07 Name two types of passive transport and three types of active transport.
Section Number: 07.01
Topic: Microbial Nutrition
72. In a commensal relationship, the commensal benefits but the cohabitant is neither harmed
nor benefited.
TRUE
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 1. Remember
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 2. Understand
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
74. The majority of microbes live and grow in habitats between pH 7 and 9.
FALSE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.14 Define doubling time, and describe how it leads to exponential growth.
Section Number: 07.03
Topic: Microbial Growth
76. The time it takes for a freshly inoculated agar culture to develop visible colonies is
primarily governed by the generation time of that species.
TRUE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 2. Understand
Learning Outcome: 07.14 Define doubling time, and describe how it leads to exponential growth.
Section Number: 07.03
Topic: Microbial Growth
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.13 Summarize the steps of cell division used by most bacteria; describe another method used by fewer bacterial
species.
Section Number: 07.03
Topic: Microbial Growth
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.15 Compare and contrast the four phases of growth in a bacterial growth curve.
Section Number: 07.03
Topic: Microbial Growth
79. The degree of turbidity in a broth culture correlates to the amount of cell growth.
TRUE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.16 Identify one quantitative and one qualitative method used for analyzing bacterial growth.
Section Number: 07.03
Topic: Microbial Growth
80. The direct cell count, using a cytometer, can calculate viable cell numbers from a sample
without the use of dyes.
FALSE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 1. Remember
Learning Outcome: 07.16 Identify one quantitative and one qualitative method used for analyzing bacterial growth.
Section Number: 07.03
Topic: Microbial Growth
81. A Coulter counter can count viable cells as well as determine the size.
FALSE
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
82. Bacterial biofilms stimulate the immune system differently than planktonic bacteria.
TRUE
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 2. Understand
Learning Outcome: 07.12 Discuss characteristics of biofilms that differentiate them from planktonic bacteria and their infections.
Section Number: 07.02
Topic: Microbial Ecology
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 2. Understand
Learning Outcome: 07.12 Discuss characteristics of biofilms that differentiate them from planktonic bacteria and their infections.
Section Number: 07.02
Topic: Microbial Ecology
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.03 List and define four different terms that describe an organism's sources of carbon and energy.
Section Number: 07.01
Topic: Microbial Nutrition
85. Which of the following environmental conditions would be inappropriate for isolation of
extremophilic microorganisms?
A. mud, with 0% oxygen content
B. a yogurt container, pH 4.7
C. a compost pile with decaying vegatables and grass, temperature of 55 degree celsius
D. seawater, NaCl content of 3.5%
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.01
Topic: Microbial Growth
86. You want to experiment with microbial growth in various nutrients, so you inoculate a
loopful of E. coli into a tube of honey. Which statement best describes what you expect to
happen and why?
A. The bacterium grows incredibly well in the honey because fructose is such a great nutrient
for bacteria.
B. The bacteria do not grow because they have been dehydrated by the honey.
C. The bacteria will not growth because the excessive sugar is directly toxic to the bacteria.
D. The bacteria do not growth because there is only sugar in the honey, without other
nutrients present they cannot survive.
ASM Objective: 03.04 The growth of microorganisms can be controlled by physical, chemical, mechanical, or biological means.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 4. Analyze
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.02
Topic: Microbial Growth
87. Which organism below has a problem in maintaining cellular electrolyte and fluid balance
in its environment?
A. a protozoan in a fresh-water lake
B. a bacterium in a fresh-water lake
C. a bacterium in Great Salt Lake
D. all of the above have problems in maintaining electrolyte and fluid balance within the cell
ASM Objective: 05.01 Microorganisms are ubiquitous and live in diverse and dynamic ecosystems.
ASM Objective: 06.02 Microorganisms provide essential models that give us fundamental knowledge about life processes.
ASM Topic: Module 05 Systems
ASM Topic: Module 06 Impact of Microorganisms
Blooms Level: 3. Apply
Learning Outcome: 07.06 Identify the effects of isotonic, hypotonic, and hypertonic conditions on a cell.
Section Number: 07.01
Topic: Environmental Influences
Topic: Microbial Ecology
88. What statement can one make about microbial growth within food in the refrigerator?
A. There is no growth of microorganisms in food in the refrigerator: the cold temperature
kills or inactivates microbial cells.
B. The absence of light in this storage condition greatly reduces the growth of most microbial
pathogens.
C. Most food does not have enough oxygen content for most microorganisms to thrive.
D. The growth of the microorganisms in the food depends on their optimal growth
temperatures: some grow slow and others grow faster.
ASM Objective: 03.04 The growth of microorganisms can be controlled by physical, chemical, mechanical, or biological means.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
89. The milk that you bought from a grocery store a month ago got pushed to the back of the
refrigerator hidden by other objects. Finding it a month later, unopened, you would like to
now use it. Which statement is true?
A. The milk will still be fresh since it is a sterilized product.
B. Only thermoduric bacteria will be thriving in the milk.
C. The milk will still be fresh since the lactose sugar prevents the growth of any
microorganisms.
D. The milk will be spoiled, making you realize you should be purchased raw milk for it
would still be fresh since it contains no microorganisms.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Objective: 03.04 The growth of microorganisms can be controlled by physical, chemical, mechanical, or biological means.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Section Number: 07.02
Topic: Environmental Influences
90. A bacterium is inoculated into a tube of nutrient broth and incubated. After incubation, it
appears turbid throughout, top to bottom, with the most amount of turbidty at the very top.
This bacterium would be called a:
A. aerotolerant
B. microaerophile
C. obligate aerobe
D. facultative anaerobe
E. psychrotroph
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 4. Analyze
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
91. You inoculate the bacterium into 3 tubes of nutrient broth medium containing salt--0.5%
NaCl, 5% NaCl, and 15% NaCl. After incubation, you notice that the organism is growing
very well in the 15%, and somewhat in the 5%. There is no growth in the third tube. This
organism would be called:
A. haloduric
B. osmophobic
C. halophilic
D. barophilic
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.02
Topic: Environmental Influences
92. You have inoculated your Clostridium species into a tube of media. After incubation, you
find that it is not growing and you then realize that this organism is an obligate anaerobe.
What is the best explanation for this lack of growth?
A. Clostridium does not have enzymes to break down hydrogen peroxide.
B. There is not enough oxygen for use when hydrogen peroxide is present.
C. There is not enough water in the environment for Clostridium to grow.
D. Oxygen in the medium destroys the enzymes needed by Clostridium to undergo aerobic
respiration.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.09 Summarize three ways in which microorganisms function in the presence of oxygen.
Section Number: 07.02
Topic: Environmental Influences
93. After using the jelly on a sandwich, you close the jar and inadvertently place it on a room
temperature shelf instead of in the refrigerator. When looking for the jelly a couple of weeks
later and finding it on the shelf, you notice growth of a microorganism on the top of the jelly.
What type of organism is likely to grow in this environment?
A. a bacterium
B. a protozoan
C. a fungus
D. a virus
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 4. Analyze
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Section Number: 07.02
Topic: Environmental Influences
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in beneficial, neutral or
detrimental ways.
ASM Topic: Module 05 Systems
Blooms Level: 2. Understand
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
95. Which of the following statements is the best explanation of why communities of
microorganisms in biofilms are synergistic.
A. The various microbial species in the biofilm interact with each other.
B. One microbial species may produce a metabolic end product that can be used as a
substrate by another species.
C. The different microbial species can all grow together.
D. The various microbial species can change the environment to their growth advantage,
allowing all to grow better.
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 4. Analyze
Learning Outcome: 07.12 Discuss characteristics of biofilms that differentiate them from planktonic bacteria and their infections.
Section Number: 07.02
Topic: Microbial Ecology
96. The termite requires the protozoan Trichonympha in its gut to provide the enzyme
cellulase for the termite's use of cellulose in wood products. The protozoan uses the end
products of the cellulose breakdown. This would be a _____ relationship.
A. commensualistic
B. mutualistic
C. parasitic
D. antagonistic
ASM Objective: 05.03 Microorganisms and their environment interact with and modify each other.
ASM Topic: Module 05 Systems
Blooms Level: 3. Apply
Learning Outcome: 07.11 List and describe the five major types of microbial association.
Section Number: 07.02
Topic: Microbial Ecology
97. A loopful of bacteria containing 1000 bacterial cells is inoculated into a nutrient broth and
incubated. The culture was in lag phase for 10 minutes and then went into exponential log
phase growth for 5 hours. The generation time for the bacterium is 15 minutes. Which
equation set up is the correct one to determine how many cells at the end of the 5 hours of log
phase growth.
A. 1000 X 220
B. 1000 X 40
C. 1000 X 24
D. 1000 X 5 X 2 X 15
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 3. Apply
Learning Outcome: 07.14 Define doubling time, and describe how it leads to exponential growth.
Section Number: 07.03
Topic: Microbial Growth
98. You have a tube of E. coli in nutrient broth that has been growing in a 37 degree C
incubator. This culture is then inoculated into 3 tubes of media:
Tube B is lactose broth, warmed to room temperature, inoculated, and then placed at
37oC.
Tube C is nutrient broth taken right out of the refrigerator, inoculated, and then placed at
37oC.
Tube D is nutrient broth, warmed to room temperature, inoculated, and then placed at
37oC.
The original tube of E. coli (called tube A) is placed back into the 37oC for incubation. In the
next one-half hour, which culture will grow the fastest and why?
A. Tube A will grow fastest because it is the original culture and has already been growing.
B. Tube B will grow fastest because lactose is a good nutrient for all bacterial species.
C. Tube C will grow fastest because the medium has been stored cold, and is fresher than the
other media used.
D. Tube D will grow fastest because the nutrients and the temperature are optimal for a fast
lag phase.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Blooms Level: 4. Analyze
Learning Outcome: 07.08 List and define five terms used to express a microbe's optimal growth temperature.
Learning Outcome: 07.10 Identify three other physical factors that microbes must contend with in the environment.
Learning Outcome: 07.15 Compare and contrast the four phases of growth in a bacterial growth curve.
Section Number: 07.03
Topic: Environmental Influences
Topic: Microbial Growth