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2008,
,
www.rjptonline.org
ISSN 0974-3618
RESEARCH ARTICLE
ABSTRACT
A simple, reproducible and efficient reverse phase high performance liquid chromatography (RP-HPLC) method has been
developed for estimation of Levetiracetam in its tablet dosage form. Separation was done by using mobile phase consists
of buffer solution (pH 2.8) and acetonitrile in the ratio of 90:10. Chromatography separations were carried out on
prontosil C18 column (150X4.6mn; 5m) at a flow rate of 1.2 ml/min and UV detection at 215nm and the retention time
for levetiracetam is 4minutes. The linear dynamic response was found to be in the concentration of 45g-270g/ml. The
slope, intercept and correlation coefficient were found to be 0.9999 respectively. The percentage recovery of
Levetiracetam was found to be 99.08%. Proposed methods were found to be simple, accurate, precise and rapid and could
be used for routine analysis. This condition is applied only for tablet dosage form. The statistical parameters and recovery
studies were carried out and reported.
EXPERIMENTAL:
N
H3CH2C
H2NOC
Received on 29.07.2008
Accepted on 14.10.2008
Modified on 06.08.2008
RJPT All right reserved
Chromatographic conditions:
The mobile phase was pH 2.8 buffer solution and
acetonitrile in the ratio of 90:10(v/v). Mobile phase was
filtered through 0.45m membrane filter and sonicated
before use. The mobile phase was pumped from the solvent
reservoir in the ratio of 90:10(v/v) to the column at a flow
rate of 1.2ml/min. The run time was set at 8 minutes. The
column was maintained at ambient temperature and the
volume of each injection was 10l. Samples were injected
in to the column and the absorptivity of the mobile phase
was monitored at 215nm using a variable wavelength
detector.
Assay procedure:
Preparation of pH 2.8 Buffer solution
Potassiumdihydrogen phosphate (0.01mol, 1.36gm) and
Sodium-1- heptane sulphonate (0,61gm) in 1000ml of milli
395
performance
parameters
of
Average area
45-270g/ml
0.9999
12438
6474.3x
Spike
level
%
25
50
75
100
150
Amount
of
Levetirac
etam
187.5
375
562.5
750
1125
Recovery
from
drug solution
Recovery
formulation
Mean
amount
Mean
amount
189.5
378.6
567.01
755.6
1133.88
Mean
(%)
recove
ry
98.34
97.09
97.51
99.13
97.70
185.52
368.21
576.85
745.63
1120.57
from
Mean(%)
recovery
97.22
97.15
101.41
98.42
97.06
Peak area
1173908
1172850
396
ACKNOWLEDGEMENTS:
The authors thank Orchid Chemicals and
Pharmaceuticals Ltd; Chennai, India for providing a
gift sample of levetiracetam. The authors are also
thankful to Mr. V. Shanmugam, Chairman, Nandha
College of pharmacy, Erode-52, Tamilnadu, India for
providing facilities for the research work
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397