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(b) Stagnant mobile phase: the solvent which is near the surface of
the support or in it pores and which does not travel freely through
the column (it is significant in liquid chromatography).
p =
e =
(C) Total porosity (e): the total fraction of the column volume
occupied by solvent.
tot =
For most porous supports, tot ~ 0.8 (I.e., 80% of the average
column is occupied by mobile phase and 20% by the solid
support.
4. Flow in packed beds, as in open tubes, can be characterized by
Question:
25 cm x(R4.1
mm ID liquid chromatography column is packed
ReynoldsAnumber
e), where dp is now set equal to the diameter of
with
sphericalin
particles
that bed.
are 10Inmicrons
in diameter
and pore
the particles
the packed
order words,
for a packed
bedsize
the
range
of 8width
nm ~of
10each
nm. flow
The channel
mobile phase
is water,
at 1 mL/min.
average
is assumed
to flowing
be the same
as the
Two
aqueous
samplesparticles.
are injected separately onto the column. This
width
of one support
first contains acetone, which eluted at 2.57 min. The second contrains a
R = u dp/
large polymer, which elutes ate 1.16 min.
Neither of these solutes
3) the bed.
interacts
with the
spherical
particles
Where:
= solvent
density
(g/cmin
u = solvent
velocity
What are the values
of thelinear
intra-,
inter, (cm/sec)
and total porosities of this
of the particles
in velocity
a packed-bed
system(cm)
dp = diameter
system? What would
you predict
the linear
of water
to be in this
= solvent viscosity (poise)
system?
Open tube
Packed bed